Cla005140 (gene) Watermelon (97103) v1

NameCla005140
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionPentatricopeptide repeat protein 65 (AHRD V1 ***- F5CAD8_FUNHY); contains Interpro domain(s) IPR002885 Pentatricopeptide repeat
LocationChr3 : 28779089 .. 28780774 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTGTTCATGGTGATCAAATTCATTCTTTGGTTTGGAAACATGGGTACCATGCTGAGGTATTTGTTGCCGGTGCATTAGTTGACATGTATGCCAAGTGTTGTGATATGTGCATGGCCGAGAAGGTGTTTGAGGAAATGCCCGTGAGAAACCTTGTGTCTTGGAACTCTATGATTGTGGGGTTCTTGCAGAACAAGCTTTATGATCAGGCCATTCTCTTTTTTAAGAGGCTTCTAGTGAAGAGTCTAACTGCTCTTGATGAGGTAAGCTTTTCCAGTGCCTTGAGTGCTTGTGCCAATGTTGATAACTTGGAGTTTGGGAAACAAGTTCATGGAGTTGCTCTGAAGCTTGGTGTCAGGAATTTGGTCTATATAAATAACTCACTGATTGACATGTATAGTAAATGTGGCTTATTTGACGACGTTGTGAAGTTGTTTTCAAACATTGAAGTTAGAGATGTTGTTACGTGGAACATAATGATCATGGCATGTGTTTATAATGACAATTATGAAGATGCCTGCAATAGTTTTTGGATGATGAGGAGGGAAGGTTTAATACCTGATGAAGCATCATACTCCTCTGTTCTTCATTCTTGTGCAAATCTTGCAGCACTATATCAGGGAACACTGATCCACAATCAGATCATACGATCTGGGTTTGTCAAGAATTTGTGTGTTGCAAGTTCTTTGATTACAATGTATGCAAAATGCGGCAGCTTGGTAGATGCTTTTCAGATATTTGAAGAGACTCAGGACCGTAATGTGGTTTGTTGGACAGCTATAATTACAGCTTGTCAACAACACGGCCATGCTAACCGGGTTGTTGAGTTATTCGAGCAAATGTTGAGAGAGGGGATTAAACCCGACTATATTACTTTTGTTTCTGTTCTCTCTGCTTGCAGCCACACTGGTCGGGTGCAAGAAGGATTCTTCTACTTTAATTCAATGATTAAAGTGCATGGTATTTACCCTGGATATGAACATTATGCATGTATAGTTGACTTGCTTGGTCGTGCTGGGCAGTTGGATAGAGCTAAGAGGTTTATAGAATTGATGCCTATCAAACCAGATGCCTCAGTATGGGGTGCTCTGCTTAGTGCTTGCAGGAATCATAGCAACCTTGAAATGGGTAAGGAAGTGGCGCTGGAACTTTTCGATTTGGAACCAGATAATCCTGGAAATTATGTGCTGCTTCGTAACATCTTGACACGTAATGGGATGTTAAATGAGGCTGATGAGGTTAGAAGAAAGATGGAAACCATGGGGGTGAGGAAGGAACCAGGATGTAGCTGGATAGACATAAAGAATTCAACATTTGTCTTCACGGTGAATGATAAGTCACATGAGAAAACGCAGGAGATTTATGAAATGTTGGAAAAGCTACGGGAGTTAGTAAAGAAGAAGGGGTATGTGGCTGAAACTGAATTTGCAATAAACATTGCAGAAGCATACAAAGAGCAAAGTTTGTGGTACCATAGTGAGAAACTAGCTCTTGCATTTGGGCTGCTGACCCTTCCTGCTGGGGCTCCAATTAGGATAAAAAAGAATCTAAGAACTTGTGGAGATTGTCATACCGTGATGAAGTTTGCTTCAGAAATTTTTGCCCGAGAGATTATAGTAAGAGATATAAGCAGATTCCACCACTTCACGAATGGCATTTGTTCCTGCAGAGATTACTGGTGA

mRNA sequence

ATGGCTTCTGTTCATGGTGATCAAATTCATTCTTTGGTTTGGAAACATGGGTACCATGCTGAGGTATTTGTTGCCGGTGCATTAGTTGACATGTATGCCAAGTGTTGTGATATGTGCATGGCCGAGAAGGTGTTTGAGGAAATGCCCGTGAGAAACCTTGTGTCTTGGAACTCTATGATTGTGGGGTTCTTGCAGAACAAGCTTTATGATCAGGCCATTCTCTTTTTTAAGAGGCTTCTAGTGAAGAGTCTAACTGCTCTTGATGAGGTAAGCTTTTCCAGTGCCTTGAGTGCTTGTGCCAATGTTGATAACTTGGAGTTTGGGAAACAAGTTCATGGAGTTGCTCTGAAGCTTGGTGTCAGGAATTTGGTCTATATAAATAACTCACTGATTGACATGTATAGTAAATGTGGCTTATTTGACGACGTTGTGAAGTTGTTTTCAAACATTGAAGTTAGAGATGTTGTTACGTGGAACATAATGATCATGGCATGTGTTTATAATGACAATTATGAAGATGCCTGCAATAGTTTTTGGATGATGAGGAGGGAAGGTTTAATACCTGATGAAGCATCATACTCCTCTGTTCTTCATTCTTGTGCAAATCTTGCAGCACTATATCAGGGAACACTGATCCACAATCAGATCATACGATCTGGGTTTGTCAAGAATTTGTGTGTTGCAAGTTCTTTGATTACAATGTATGCAAAATGCGGCAGCTTGGTAGATGCTTTTCAGATATTTGAAGAGACTCAGGACCGTAATGTGGTTTGTTGGACAGCTATAATTACAGCTTGTCAACAACACGGCCATGCTAACCGGGTTGTTGAGTTATTCGAGCAAATGTTGAGAGAGGGGATTAAACCCGACTATATTACTTTTGTTTCTGTTCTCTCTGCTTGCAGCCACACTGGTCGGGTGCAAGAAGGATTCTTCTACTTTAATTCAATGATTAAAGTGCATGGTATTTACCCTGGATATGAACATTATGCATGTATAGTTGACTTGCTTGGTCGTGCTGGGCAGTTGGATAGAGCTAAGAGGTTTATAGAATTGATGCCTATCAAACCAGATGCCTCAGTATGGGGTGCTCTGCTTAGTGCTTGCAGGAATCATAGCAACCTTGAAATGGGTAAGGAAGTGGCGCTGGAACTTTTCGATTTGGAACCAGATAATCCTGGAAATTATGTGCTGCTTCGTAACATCTTGACACGTAATGGGATGTTAAATGAGGCTGATGAGGTTAGAAGAAAGATGGAAACCATGGGGGTGAGGAAGGAACCAGGATGTAGCTGGATAGACATAAAGAATTCAACATTTGTCTTCACGGTGAATGATAAGTCACATGAGAAAACGCAGGAGATTTATGAAATGTTGGAAAAGCTACGGGAGTTAGTAAAGAAGAAGGGGTATGTGGCTGAAACTGAATTTGCAATAAACATTGCAGAAGCATACAAAGAGCAAAGTTTGTGGTACCATAGTGAGAAACTAGCTCTTGCATTTGGGCTGCTGACCCTTCCTGCTGGGGCTCCAATTAGGATAAAAAAGAATCTAAGAACTTGTGGAGATTGTCATACCGTGATGAAGTTTGCTTCAGAAATTTTTGCCCGAGAGATTATAGTAAGAGATATAAGCAGATTCCACCACTTCACGAATGGCATTTGTTCCTGCAGAGATTACTGGTGA

Coding sequence (CDS)

ATGGCTTCTGTTCATGGTGATCAAATTCATTCTTTGGTTTGGAAACATGGGTACCATGCTGAGGTATTTGTTGCCGGTGCATTAGTTGACATGTATGCCAAGTGTTGTGATATGTGCATGGCCGAGAAGGTGTTTGAGGAAATGCCCGTGAGAAACCTTGTGTCTTGGAACTCTATGATTGTGGGGTTCTTGCAGAACAAGCTTTATGATCAGGCCATTCTCTTTTTTAAGAGGCTTCTAGTGAAGAGTCTAACTGCTCTTGATGAGGTAAGCTTTTCCAGTGCCTTGAGTGCTTGTGCCAATGTTGATAACTTGGAGTTTGGGAAACAAGTTCATGGAGTTGCTCTGAAGCTTGGTGTCAGGAATTTGGTCTATATAAATAACTCACTGATTGACATGTATAGTAAATGTGGCTTATTTGACGACGTTGTGAAGTTGTTTTCAAACATTGAAGTTAGAGATGTTGTTACGTGGAACATAATGATCATGGCATGTGTTTATAATGACAATTATGAAGATGCCTGCAATAGTTTTTGGATGATGAGGAGGGAAGGTTTAATACCTGATGAAGCATCATACTCCTCTGTTCTTCATTCTTGTGCAAATCTTGCAGCACTATATCAGGGAACACTGATCCACAATCAGATCATACGATCTGGGTTTGTCAAGAATTTGTGTGTTGCAAGTTCTTTGATTACAATGTATGCAAAATGCGGCAGCTTGGTAGATGCTTTTCAGATATTTGAAGAGACTCAGGACCGTAATGTGGTTTGTTGGACAGCTATAATTACAGCTTGTCAACAACACGGCCATGCTAACCGGGTTGTTGAGTTATTCGAGCAAATGTTGAGAGAGGGGATTAAACCCGACTATATTACTTTTGTTTCTGTTCTCTCTGCTTGCAGCCACACTGGTCGGGTGCAAGAAGGATTCTTCTACTTTAATTCAATGATTAAAGTGCATGGTATTTACCCTGGATATGAACATTATGCATGTATAGTTGACTTGCTTGGTCGTGCTGGGCAGTTGGATAGAGCTAAGAGGTTTATAGAATTGATGCCTATCAAACCAGATGCCTCAGTATGGGGTGCTCTGCTTAGTGCTTGCAGGAATCATAGCAACCTTGAAATGGGTAAGGAAGTGGCGCTGGAACTTTTCGATTTGGAACCAGATAATCCTGGAAATTATGTGCTGCTTCGTAACATCTTGACACGTAATGGGATGTTAAATGAGGCTGATGAGGTTAGAAGAAAGATGGAAACCATGGGGGTGAGGAAGGAACCAGGATGTAGCTGGATAGACATAAAGAATTCAACATTTGTCTTCACGGTGAATGATAAGTCACATGAGAAAACGCAGGAGATTTATGAAATGTTGGAAAAGCTACGGGAGTTAGTAAAGAAGAAGGGGTATGTGGCTGAAACTGAATTTGCAATAAACATTGCAGAAGCATACAAAGAGCAAAGTTTGTGGTACCATAGTGAGAAACTAGCTCTTGCATTTGGGCTGCTGACCCTTCCTGCTGGGGCTCCAATTAGGATAAAAAAGAATCTAAGAACTTGTGGAGATTGTCATACCGTGATGAAGTTTGCTTCAGAAATTTTTGCCCGAGAGATTATAGTAAGAGATATAAGCAGATTCCACCACTTCACGAATGGCATTTGTTCCTGCAGAGATTACTGGTGA

Protein sequence

MASVHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMGVRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAEAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRDISRFHHFTNGICSCRDYW
BLAST of Cla005140 vs. Swiss-Prot
Match: PP252_ARATH (Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidopsis thaliana GN=PCMP-H87 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.2e-131
Identity = 239/559 (42.75%), Postives = 352/559 (62.97%), Query Frame = 1

Query: 4   VHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGF 63
           + G  +H+ + +  +  ++ +   L++MYAKC  +  A KVFE+MP R+ V+W ++I G+
Sbjct: 77  IQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLEEARKVFEKMPQRDFVTWTTLISGY 136

Query: 64  LQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNL 123
            Q+     A+LFF ++L    +  +E + SS + A A       G Q+HG  +K G  + 
Sbjct: 137 SQHDRPCDALLFFNQMLRFGYSP-NEFTLSSVIKAAAAERRGCCGHQLHGFCVKCGFDSN 196

Query: 124 VYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRR 183
           V++ ++L+D+Y++ GL DD   +F  +E R+ V+WN +I         E A   F  M R
Sbjct: 197 VHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLR 256

Query: 184 EGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVD 243
           +G  P   SY+S+  +C++   L QG  +H  +I+SG        ++L+ MYAK GS+ D
Sbjct: 257 DGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHD 316

Query: 244 AFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSH 303
           A +IF+    R+VV W +++TA  QHG     V  FE+M R GI+P+ I+F+SVL+ACSH
Sbjct: 317 ARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGIRPNEISFLSVLTACSH 376

Query: 304 TGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWG 363
           +G + EG+ Y+  M K  GI P   HY  +VDLLGRAG L+RA RFIE MPI+P A++W 
Sbjct: 377 SGLLDEGWHYYELM-KKDGIVPEAWHYVTVVDLLGRAGDLNRALRFIEEMPIEPTAAIWK 436

Query: 364 ALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMG 423
           ALL+ACR H N E+G   A  +F+L+PD+PG +V+L NI    G  N+A  VR+KM+  G
Sbjct: 437 ALLNACRMHKNTELGAYAAEHVFELDPDDPGPHVILYNIYASGGRWNDAARVRKKMKESG 496

Query: 424 VRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAI-NIA 483
           V+KEP CSW++I+N+  +F  ND+ H + +EI    E++   +K+ GYV +T   I ++ 
Sbjct: 497 VKKEPACSWVEIENAIHMFVANDERHPQREEIARKWEEVLAKIKELGYVPDTSHVIVHVD 556

Query: 484 EAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVR 543
           +  +E +L YHSEK+ALAF LL  P G+ I IKKN+R CGDCHT +K AS++  REIIVR
Sbjct: 557 QQEREVNLQYHSEKIALAFALLNTPPGSTIHIKKNIRVCGDCHTAIKLASKVVGREIIVR 616

Query: 544 DISRFHHFTNGICSCRDYW 562
           D +RFHHF +G CSC+DYW
Sbjct: 617 DTNRFHHFKDGNCSCKDYW 633

BLAST of Cla005140 vs. Swiss-Prot
Match: PP168_ARATH (Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana GN=PCMP-H41 PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 7.9e-129
Identity = 255/622 (41.00%), Postives = 359/622 (57.72%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPV--------------- 65
           G ++HS + K G    V V+ +L++MYAKC D  MA+ VF+ M V               
Sbjct: 165 GKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQ 224

Query: 66  ----------------RNLVSWNSMIVGFLQNKLYDQAILFFKRLLVKSLTALDEVSFSS 125
                           R++V+WNSMI GF Q     +A+  F ++L  SL + D  + +S
Sbjct: 225 VGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLAS 284

Query: 126 ALSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKCG---------------- 185
            LSACAN++ L  GKQ+H   +  G      + N+LI MYS+CG                
Sbjct: 285 VLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKD 344

Query: 186 --------LFDDVVKL---------FSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMM 245
                   L D  +KL         F +++ RDVV W  MI+    + +Y +A N F  M
Sbjct: 345 LKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSM 404

Query: 246 RREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSL 305
              G  P+  + +++L   ++LA+L  G  IH   ++SG + ++ V+++LITMYAK G++
Sbjct: 405 VGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNI 464

Query: 306 VDAFQIFEETQ-DRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA 365
             A + F+  + +R+ V WT++I A  QHGHA   +ELFE ML EG++PD+IT+V V SA
Sbjct: 465 TSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSA 524

Query: 366 CSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDAS 425
           C+H G V +G  YF+ M  V  I P   HYAC+VDL GRAG L  A+ FIE MPI+PD  
Sbjct: 525 CTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVV 584

Query: 426 VWGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKME 485
            WG+LLSACR H N+++GK  A  L  LEP+N G Y  L N+ +  G   EA ++R+ M+
Sbjct: 585 TWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSMK 644

Query: 486 TMGVRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAI- 545
              V+KE G SWI++K+   VF V D +H +  EIY  ++K+ + +KK GYV +T   + 
Sbjct: 645 DGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVLH 704

Query: 546 NIAEAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREI 562
           ++ E  KEQ L +HSEKLA+AFGL++ P    +RI KNLR C DCHT +KF S++  REI
Sbjct: 705 DLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCNDCHTAIKFISKLVGREI 764


HSP 2 Score: 99.4 bits (246), Expect = 1.4e-19
Identity = 92/375 (24.53%), Postives = 152/375 (40.53%), Query Frame = 1

Query: 90  VSFSSALSACANV-------DNLEFGKQ-VHGVALKLGVRNLVYINNSLIDMYSKCG--- 149
           +S S+ L  C N+        N  F  Q VH   +K G+   VY+ N+L+++YSK G   
Sbjct: 7   LSLSTLLELCTNLLQKSVNKSNGRFTAQLVHCRVIKSGLMFSVYLMNNLMNVYSKTGYAL 66

Query: 150 ----LFDDV------------------------VKLFSNIEVRDVVTWNIMIMACVYNDN 209
               LFD++                         + F  +  RD V+W  MI+       
Sbjct: 67  HARKLFDEMPLRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQ 126

Query: 210 YEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASS 269
           Y  A      M +EG+ P + + ++VL S A    +  G  +H+ I++ G   N+ V++S
Sbjct: 127 YHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNS 186

Query: 270 LITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPD 329
           L+ MYAKCG  + A  +F+    R++  W A+I    Q G  +  +  FEQM       D
Sbjct: 187 LLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAER----D 246

Query: 330 YITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFI 389
            +T+ S++S  +  G        F+ M++               D L             
Sbjct: 247 IVTWNSMISGFNQRGYDLRALDIFSKMLR---------------DSL------------- 306

Query: 390 ELMPIKPDASVWGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLN 426
               + PD     ++LSAC N   L +GK++   +     D  G  ++L  +++      
Sbjct: 307 ----LSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISG--IVLNALISMYSRCG 343


HSP 3 Score: 84.3 bits (207), Expect = 4.5e-15
Identity = 86/369 (23.31%), Postives = 161/369 (43.63%), Query Frame = 1

Query: 23  FVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQNKLYDQAILFFKRLLVK 82
           F    ++  Y+K  DM    + F+++P R+ VSW +MIVG+     Y +AI     ++ +
Sbjct: 81  FSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKE 140

Query: 83  SLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKCGLFDD 142
            +    + + ++ L++ A    +E GK+VH   +KLG+R  V ++NSL++MY+KCG    
Sbjct: 141 GIEPT-QFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMM 200

Query: 143 VVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREGLIPDEASYSSVLHSCAN 202
              +F  + VRD+ +WN MI   +     + A   F  M    ++   +  S       +
Sbjct: 201 AKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYD 260

Query: 203 LAALYQGTLIHNQIIRSGFVK-NLCVASSLITMYAKCGSLVDAFQIFEETQ----DRNVV 262
           L AL     I ++++R   +  +    +S+++  A    L    QI         D + +
Sbjct: 261 LRALD----IFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGI 320

Query: 263 CWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTGRVQEGFFYFNSM 322
              A+I+   + G       L EQ   + +K +   F ++L      G + +    F S+
Sbjct: 321 VLNALISMYSRCGGVETARRLIEQRGTKDLKIE--GFTALLDGYIKLGDMNQAKNIFVSL 380

Query: 323 -----IKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNH 382
                +    +  GYE +       G  G+     R +     +P++    A+LS   + 
Sbjct: 381 KDRDVVAWTAMIVGYEQH-------GSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSL 435


HSP 4 Score: 48.9 bits (115), Expect = 2.1e-04
Identity = 30/112 (26.79%), Postives = 54/112 (48.21%), Query Frame = 1

Query: 9   IHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQNKL 68
           +H  V K G    V++   L+++Y+K      A K+F+EMP+R   SWN+++  + +   
Sbjct: 36  VHCRVIKSGLMFSVYLMNNLMNVYSKTGYALHARKLFDEMPLRTAFSWNTVLSAYSKRGD 95

Query: 69  YDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGV 121
            D    FF +L  +     D VS+++ +    N+       +V G  +K G+
Sbjct: 96  MDSTCEFFDQLPQR-----DSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGI 142

BLAST of Cla005140 vs. Swiss-Prot
Match: PP251_ARATH (Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis thaliana GN=PCMP-H32 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 9.7e-127
Identity = 230/593 (38.79%), Postives = 351/593 (59.19%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKC-----------------------------C 65
           G+ +H  + + G   +++   AL++MYAK                               
Sbjct: 124 GESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKISVGNVFDEMPQRTSNSGDEDVKA 183

Query: 66  DMCM-------AEKVFEEMPVRNLVSWNSMIVGFLQNKLYDQAILFFKRLLVKSLTALDE 125
           + C+         +VFE MP +++VS+N++I G+ Q+ +Y+ A+   + +    L   D 
Sbjct: 184 ETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKP-DS 243

Query: 126 VSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKCGLFDDVVKLFSN 185
            + SS L   +   ++  GK++HG  ++ G+ + VYI +SL+DMY+K    +D  ++FS 
Sbjct: 244 FTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSR 303

Query: 186 IEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQG 245
           +  RD ++WN ++   V N  Y +A   F  M    + P   ++SSV+ +CA+LA L+ G
Sbjct: 304 LYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLG 363

Query: 246 TLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQH 305
             +H  ++R GF  N+ +AS+L+ MY+KCG++  A +IF+     + V WTAII     H
Sbjct: 364 KQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALH 423

Query: 306 GHANRVVELFEQMLREGIKPDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEH 365
           GH +  V LFE+M R+G+KP+ + FV+VL+ACSH G V E + YFNSM KV+G+    EH
Sbjct: 424 GHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQELEH 483

Query: 366 YACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNHSNLEMGKEVALELFDLE 425
           YA + DLLGRAG+L+ A  FI  M ++P  SVW  LLS+C  H NLE+ ++VA ++F ++
Sbjct: 484 YAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVHKNLELAEKVAEKIFTVD 543

Query: 426 PDNPGNYVLLRNILTRNGMLNEADEVRRKMETMGVRKEPGCSWIDIKNSTFVFTVNDKSH 485
            +N G YVL+ N+   NG   E  ++R +M   G+RK+P CSWI++KN T  F   D+SH
Sbjct: 544 SENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEMKNKTHGFVSGDRSH 603

Query: 486 EKTQEIYEMLEKLRELVKKKGYVAETEFAI-NIAEAYKEQSLWYHSEKLALAFGLLTLPA 545
               +I E L+ + E ++K+GYVA+T   + ++ E +K + L+ HSE+LA+AFG++    
Sbjct: 604 PSMDKINEFLKAVMEQMEKEGYVADTSGVLHDVDEEHKRELLFGHSERLAVAFGIINTEP 663

Query: 546 GAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRDISRFHHFTNGICSCRDYW 562
           G  IR+ KN+R C DCH  +KF S+I  REIIVRD SRFHHF  G CSC DYW
Sbjct: 664 GTTIRVTKNIRICTDCHVAIKFISKITEREIIVRDNSRFHHFNRGNCSCGDYW 715


HSP 2 Score: 134.8 bits (338), Expect = 2.9e-30
Identity = 101/399 (25.31%), Postives = 180/399 (45.11%), Query Frame = 1

Query: 25  AGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQNKLYDQAILFFKRLLVKSL 84
           A  ++ +Y     +  A  +F+ +    +++W S+I  F    L+ +A+  F  +     
Sbjct: 42  ASIVISIYTNLKLLHEALLLFKTLKSPPVLAWKSVIRCFTDQSLFSKALASFVEMRASGR 101

Query: 85  TALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKC------- 144
              D   F S L +C  + +L FG+ VHG  ++LG+   +Y  N+L++MY+K        
Sbjct: 102 CP-DHNVFPSVLKSCTMMMDLRFGESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKI 161

Query: 145 ---GLFDDVVKLFSN-----------------------IEV---RDVVTWNIMIMACVYN 204
               +FD++ +  SN                        EV   +DVV++N +I     +
Sbjct: 162 SVGNVFDEMPQRTSNSGDEDVKAETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQS 221

Query: 205 DNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVA 264
             YEDA      M    L PD  + SSVL   +    + +G  IH  +IR G   ++ + 
Sbjct: 222 GMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIG 281

Query: 265 SSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIK 324
           SSL+ MYAK   + D+ ++F     R+ + W +++    Q+G  N  + LF QM+   +K
Sbjct: 282 SSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVK 341

Query: 325 PDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKR 384
           P  + F SV+ AC+H   +  G      +++  G        + +VD+  + G +  A++
Sbjct: 342 PGAVAFSSVIPACAHLATLHLGKQLHGYVLR-GGFGSNIFIASALVDMYSKCGNIKAARK 401

Query: 385 FIELMPIKPDASVWGALLSACRNHSNLEMGKEVALELFD 388
             + M +  + S W A++     H +   G E A+ LF+
Sbjct: 402 IFDRMNVLDEVS-WTAIIMGHALHGH---GHE-AVSLFE 433

BLAST of Cla005140 vs. Swiss-Prot
Match: PP307_ARATH (Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN=PCMP-H42 PE=2 SV=2)

HSP 1 Score: 454.5 bits (1168), Expect = 1.7e-126
Identity = 228/557 (40.93%), Postives = 335/557 (60.14%), Query Frame = 1

Query: 6    GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
            G+QIHS + K  +    +V   L+DMYAK   +  A  +      +++VSW +MI G+ Q
Sbjct: 509  GEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQ 568

Query: 66   NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVY 125
                D+A+  F+++L + + + DEV  ++A+SACA +  L+ G+Q+H  A   G  + + 
Sbjct: 569  YNFDDKALTTFRQMLDRGIRS-DEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLP 628

Query: 126  INNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREG 185
              N+L+ +YS+CG  ++    F   E  D + WN ++     + N E+A   F  M REG
Sbjct: 629  FQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREG 688

Query: 186  LIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAF 245
            +  +  ++ S + + +  A + QG  +H  I ++G+     V ++LI+MYAKCGS+ DA 
Sbjct: 689  IDNNNFTFGSAVKAASETANMKQGKQVHAVITKTGYDSETEVCNALISMYAKCGSISDAE 748

Query: 246  QIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTG 305
            + F E   +N V W AII A  +HG  +  ++ F+QM+   ++P+++T V VLSACSH G
Sbjct: 749  KQFLEVSTKNEVSWNAIINAYSKHGFGSEALDSFDQMIHSNVRPNHVTLVGVLSACSHIG 808

Query: 306  RVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGAL 365
             V +G  YF SM   +G+ P  EHY C+VD+L RAG L RAK FI+ MPIKPDA VW  L
Sbjct: 809  LVDKGIAYFESMNSEYGLSPKPEHYVCVVDMLTRAGLLSRAKEFIQEMPIKPDALVWRTL 868

Query: 366  LSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMGVR 425
            LSAC  H N+E+G+  A  L +LEP++   YVLL N+   +   +  D  R+KM+  GV+
Sbjct: 869  LSACVVHKNMEIGEFAAHHLLELEPEDSATYVLLSNLYAVSKKWDARDLTRQKMKEKGVK 928

Query: 426  KEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAIN-IAEA 485
            KEPG SWI++KNS   F V D++H    EI+E  + L +   + GYV +    +N +   
Sbjct: 929  KEPGQSWIEVKNSIHSFYVGDQNHPLADEIHEYFQDLTKRASEIGYVQDCFSLLNELQHE 988

Query: 486  YKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRDI 545
             K+  ++ HSEKLA++FGLL+LPA  PI + KNLR C DCH  +KF S++  REIIVRD 
Sbjct: 989  QKDPIIFIHSEKLAISFGLLSLPATVPINVMKNLRVCNDCHAWIKFVSKVSNREIIVRDA 1048

Query: 546  SRFHHFTNGICSCRDYW 562
             RFHHF  G CSC+DYW
Sbjct: 1049 YRFHHFEGGACSCKDYW 1064


HSP 2 Score: 187.6 bits (475), Expect = 3.8e-46
Identity = 120/420 (28.57%), Postives = 214/420 (50.95%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
           G Q+H+   K G+ +   + GAL+++YAKC D+  A   F E  V N+V WN M+V +  
Sbjct: 408 GQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGL 467

Query: 66  NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVY 125
                 +   F+++ ++ +   ++ ++ S L  C  + +LE G+Q+H   +K   +   Y
Sbjct: 468 LDDLRNSFRIFRQMQIEEIVP-NQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAY 527

Query: 126 INNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREG 185
           + + LIDMY+K G  D    +      +DVV+W  MI      +  + A  +F  M   G
Sbjct: 528 VCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRG 587

Query: 186 LIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAF 245
           +  DE   ++ + +CA L AL +G  IH Q   SGF  +L   ++L+T+Y++CG + +++
Sbjct: 588 IRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESY 647

Query: 246 QIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTG 305
             FE+T+  + + W A+++  QQ G+    + +F +M REGI  +  TF S + A S T 
Sbjct: 648 LAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETA 707

Query: 306 RVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQL-DRAKRFIELMPIKPDASVWGA 365
            +++G    +++I   G     E    ++ +  + G + D  K+F+E +  K + S W A
Sbjct: 708 NMKQG-KQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLE-VSTKNEVS-WNA 767

Query: 366 LLSACRNHSNLEMGKEVALELFD--LEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETM 423
           +++A   H     G E AL+ FD  +  +   N+V L  +L+    +   D+     E+M
Sbjct: 768 IINAYSKHG---FGSE-ALDSFDQMIHSNVRPNHVTLVGVLSACSHIGLVDKGIAYFESM 819


HSP 3 Score: 187.2 bits (474), Expect = 4.9e-46
Identity = 118/381 (30.97%), Postives = 193/381 (50.66%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
           G+Q+H LV K G+ ++ +V  ALV +Y    ++  AE +F  M  R+ V++N++I G  Q
Sbjct: 307 GEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQ 366

Query: 66  NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVY 125
               ++A+  FKR+ +  L   D  + +S + AC+    L  G+Q+H    KLG  +   
Sbjct: 367 CGYGEKAMELFKRMHLDGLEP-DSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNK 426

Query: 126 INNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREG 185
           I  +L+++Y+KC   +  +  F   EV +VV WN+M++A    D+  ++   F  M+ E 
Sbjct: 427 IEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEE 486

Query: 186 LIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAF 245
           ++P++ +Y S+L +C  L  L  G  IH+QII++ F  N  V S LI MYAK G L  A+
Sbjct: 487 IVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAW 546

Query: 246 QIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTG 305
            I      ++VV WT +I    Q+   ++ +  F QML  GI+ D +   + +SAC+   
Sbjct: 547 DILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQ 606

Query: 306 RVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGAL 365
            ++EG    ++   V G          +V L  R G+++ +    E      D   W AL
Sbjct: 607 ALKEG-QQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFE-QTEAGDNIAWNAL 666

Query: 366 LSACRNHSNLEMGKEVALELF 387
           +S  +   N     E AL +F
Sbjct: 667 VSGFQQSGN----NEEALRVF 680


HSP 4 Score: 172.6 bits (436), Expect = 1.3e-41
Identity = 102/340 (30.00%), Postives = 171/340 (50.29%), Query Frame = 1

Query: 7   DQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQN 66
           +QIH+ +   G      V   L+D+Y++   + +A +VF+ + +++  SW +MI G  +N
Sbjct: 207 EQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKN 266

Query: 67  KLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVYI 126
           +   +AI  F  + V  +      +FSS LSAC  +++LE G+Q+HG+ LKLG  +  Y+
Sbjct: 267 ECEAEAIRLFCDMYVLGIMPTP-YAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYV 326

Query: 127 NNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREGL 186
            N+L+ +Y   G       +FSN+  RD VT+N +I         E A   F  M  +GL
Sbjct: 327 CNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGL 386

Query: 187 IPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQ 246
            PD  + +S++ +C+    L++G  +H    + GF  N  +  +L+ +YAKC  +  A  
Sbjct: 387 EPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALD 446

Query: 247 IFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTGR 306
            F ET+  NVV W  ++ A            +F QM  E I P+  T+ S+L  C   G 
Sbjct: 447 YFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGD 506

Query: 307 VQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRA 347
           ++ G    + +IK +     Y   + ++D+  + G+LD A
Sbjct: 507 LELGEQIHSQIIKTNFQLNAYV-CSVLIDMYAKLGKLDTA 544


HSP 5 Score: 157.5 bits (397), Expect = 4.2e-37
Identity = 92/306 (30.07%), Postives = 157/306 (51.31%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
           G ++HS + K G  +   ++  L D Y    D+  A KVF+EMP R + +WN MI     
Sbjct: 104 GRKLHSQILKLGLDSNGCLSEKLFDFYLFKGDLYGAFKVFDEMPERTIFTWNKMIKELAS 163

Query: 66  NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVD-NLEFGKQVHGVALKLGVRNLV 125
             L  +    F R++ +++T  +E +FS  L AC       +  +Q+H   L  G+R+  
Sbjct: 164 RNLIGEVFGLFVRMVSENVTP-NEGTFSGVLEACRGGSVAFDVVEQIHARILYQGLRDST 223

Query: 126 YINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRRE 185
            + N LID+YS+ G  D   ++F  + ++D  +W  MI     N+   +A   F  M   
Sbjct: 224 VVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVL 283

Query: 186 GLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDA 245
           G++P   ++SSVL +C  + +L  G  +H  +++ GF  +  V ++L+++Y   G+L+ A
Sbjct: 284 GIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISA 343

Query: 246 FQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHT 305
             IF     R+ V +  +I    Q G+  + +ELF++M  +G++PD  T  S++ ACS  
Sbjct: 344 EHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSAD 403

Query: 306 GRVQEG 311
           G +  G
Sbjct: 404 GTLFRG 408

BLAST of Cla005140 vs. Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-126
Identity = 235/556 (42.27%), Postives = 338/556 (60.79%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
           G +IH    + G+ + V ++ ALVDMYAKC  +  A ++F+ M  RN+VSWNSMI  ++Q
Sbjct: 255 GKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQ 314

Query: 66  NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVY 125
           N+   +A+L F+++L + +   D VS   AL ACA++ +LE G+ +H ++++LG+   V 
Sbjct: 315 NENPKEAMLIFQKMLDEGVKPTD-VSVMGALHACADLGDLERGRFIHKLSVELGLDRNVS 374

Query: 126 INNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREG 185
           + NSLI MY KC   D    +F  ++ R +V+WN MI+    N    DA N F  MR   
Sbjct: 375 VVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRT 434

Query: 186 LIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAF 245
           + PD  +Y SV+ + A L+  +    IH  ++RS   KN+ V ++L+ MYAKCG+++ A 
Sbjct: 435 VKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIAR 494

Query: 246 QIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTG 305
            IF+   +R+V  W A+I     HG     +ELFE+M +  IKP+ +TF+SV+SACSH+G
Sbjct: 495 LIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSG 554

Query: 306 RVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGAL 365
            V+ G   F  M + + I    +HY  +VDLLGRAG+L+ A  FI  MP+KP  +V+GA+
Sbjct: 555 LVEAGLKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAM 614

Query: 366 LSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMGVR 425
           L AC+ H N+   ++ A  LF+L PD+ G +VLL NI     M  +  +VR  M   G+R
Sbjct: 615 LGACQIHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLR 674

Query: 426 KEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAEAY 485
           K PGCS ++IKN    F     +H  +++IY  LEKL   +K+ GYV +T   + +    
Sbjct: 675 KTPGCSMVEIKNEVHSFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLVLGVENDV 734

Query: 486 KEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRDIS 545
           KEQ L  HSEKLA++FGLL   AG  I ++KNLR C DCH   K+ S +  REI+VRD+ 
Sbjct: 735 KEQLLSTHSEKLAISFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGREIVVRDMQ 794

Query: 546 RFHHFTNGICSCRDYW 562
           RFHHF NG CSC DYW
Sbjct: 795 RFHHFKNGACSCGDYW 809


HSP 2 Score: 213.0 bits (541), Expect = 8.4e-54
Identity = 125/382 (32.72%), Postives = 203/382 (53.14%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
           G +IH L+ K G+  ++F    L +MYAKC  +  A KVF+ MP R+LVSWN+++ G+ Q
Sbjct: 154 GKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQ 213

Query: 66  NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVY 125
           N +   A+   K +  ++L     ++  S L A + +  +  GK++HG A++ G  +LV 
Sbjct: 214 NGMARMALEMVKSMCEENLKP-SFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVN 273

Query: 126 INNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREG 185
           I+ +L+DMY+KCG  +   +LF  +  R+VV+WN MI A V N+N ++A   F  M  EG
Sbjct: 274 ISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEG 333

Query: 186 LIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAF 245
           + P + S    LH+CA+L  L +G  IH   +  G  +N+ V +SLI+MY KC  +  A 
Sbjct: 334 VKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAA 393

Query: 246 QIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTG 305
            +F + Q R +V W A+I    Q+G     +  F QM    +KPD  T+VSV++A +   
Sbjct: 394 SMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELS 453

Query: 306 RVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGAL 365
            +     + + ++    +         +VD+  + G +  A+   ++M  +   + W A+
Sbjct: 454 -ITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMS-ERHVTTWNAM 513

Query: 366 LSACRNHSNLEMGKEVALELFD 388
           +     H     GK  ALELF+
Sbjct: 514 IDGYGTHG---FGK-AALELFE 528


HSP 3 Score: 159.8 bits (403), Expect = 8.4e-38
Identity = 95/305 (31.15%), Postives = 162/305 (53.11%), Query Frame = 1

Query: 8   QIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQNK 67
           QI  LV+K+G + E F    LV ++ +   +  A +VFE +  +  V +++M+ GF +  
Sbjct: 55  QILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVS 114

Query: 68  LYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVYIN 127
             D+A+ FF R+    +  +   +F+  L  C +   L  GK++HG+ +K G    ++  
Sbjct: 115 DLDKALQFFVRMRYDDVEPV-VYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAM 174

Query: 128 NSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREGLI 187
             L +MY+KC   ++  K+F  +  RD+V+WN ++     N     A      M  E L 
Sbjct: 175 TGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLK 234

Query: 188 PDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQI 247
           P   +  SVL + + L  +  G  IH   +RSGF   + ++++L+ MYAKCGSL  A Q+
Sbjct: 235 PSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQL 294

Query: 248 FEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTGRV 307
           F+   +RNVV W ++I A  Q+ +    + +F++ML EG+KP  ++ +  L AC+  G +
Sbjct: 295 FDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDL 354

Query: 308 QEGFF 313
           + G F
Sbjct: 355 ERGRF 358


HSP 4 Score: 109.4 bits (272), Expect = 1.3e-22
Identity = 69/283 (24.38%), Postives = 135/283 (47.70%), Query Frame = 1

Query: 96  LSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDV 155
           L  C+++  L   +Q+  +  K G+    +    L+ ++ + G  D+  ++F  I+ +  
Sbjct: 44  LERCSSLKEL---RQILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLN 103

Query: 156 VTWNIMIMACVYNDNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQ 215
           V ++ M+       + + A   F  MR + + P   +++ +L  C + A L  G  IH  
Sbjct: 104 VLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGL 163

Query: 216 IIRSGFVKNLCVASSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRV 275
           +++SGF  +L   + L  MYAKC  + +A ++F+   +R++V W  I+    Q+G A   
Sbjct: 164 LVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMA 223

Query: 276 VELFEQMLREGIKPDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEHY----A 335
           +E+ + M  E +KP +IT VSVL A S    +  G       I  + +  G++       
Sbjct: 224 LEMVKSMCEENLKPSFITIVSVLPAVSALRLISVG-----KEIHGYAMRSGFDSLVNIST 283

Query: 336 CIVDLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNHSN 375
            +VD+  + G L+ A++  + M ++ +   W +++ A   + N
Sbjct: 284 ALVDMYAKCGSLETARQLFDGM-LERNVVSWNSMIDAYVQNEN 317

BLAST of Cla005140 vs. TrEMBL
Match: A0A0A0LVV0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G560780 PE=4 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 6.3e-266
Identity = 455/501 (90.82%), Postives = 475/501 (94.81%), Query Frame = 1

Query: 62  GFL-QNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGV 121
           GFL +NKLYDQAI FFK LL+++LTALDEVSFSS  SACAN  NLEFGKQVHGVALKLGV
Sbjct: 160 GFLAENKLYDQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNLEFGKQVHGVALKLGV 219

Query: 122 RNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWM 181
            NLVYINNSL DMY KCGLF+DV KLFSN   RDVVTWNIMIMA VYN NYEDACNSFWM
Sbjct: 220 WNLVYINNSLSDMYGKCGLFNDVAKLFSNTGARDVVTWNIMIMAYVYNHNYEDACNSFWM 279

Query: 182 MRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGS 241
           MRR+G IPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNL VASSLITMYAKCGS
Sbjct: 280 MRRKGSIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLRVASSLITMYAKCGS 339

Query: 242 LVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA 301
           LVDAFQIFEET+DRNVVCWTAII ACQQHGHAN VVELFEQMLREGIKPDYITFVSVLSA
Sbjct: 340 LVDAFQIFEETEDRNVVCWTAIIAACQQHGHANWVVELFEQMLREGIKPDYITFVSVLSA 399

Query: 302 CSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDAS 361
           CSHTGRV+EGFFYFNSMIKVHGIYPG+EHYACIVDLL RAG+LDRAKRFIELMPIKPDAS
Sbjct: 400 CSHTGRVEEGFFYFNSMIKVHGIYPGHEHYACIVDLLSRAGELDRAKRFIELMPIKPDAS 459

Query: 362 VWGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKME 421
           VWGALLSACRNHSNL MGKEVAL+LFDLEPDNPGNYVLL NILTRNGMLNEADEVRRKME
Sbjct: 460 VWGALLSACRNHSNLIMGKEVALKLFDLEPDNPGNYVLLCNILTRNGMLNEADEVRRKME 519

Query: 422 TMGVRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAIN 481
           ++GVRKEPGCSWIDIKNST+VFTV+DKSHEKT+EIYEMLEKL+ELVKKKGYVAETEFAIN
Sbjct: 520 SIGVRKEPGCSWIDIKNSTYVFTVHDKSHEKTKEIYEMLEKLKELVKKKGYVAETEFAIN 579

Query: 482 IAEAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII 541
            AE YKEQSLWYHSEK+ALAFGLL+LPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII
Sbjct: 580 TAEEYKEQSLWYHSEKIALAFGLLSLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII 639

Query: 542 VRDISRFHHFTNGICSCRDYW 562
           VRDI+RFHHFTNGICSC DYW
Sbjct: 640 VRDINRFHHFTNGICSCGDYW 660

BLAST of Cla005140 vs. TrEMBL
Match: A0A0A0LVV0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G560780 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 8.5e-13
Identity = 80/329 (24.32%), Postives = 131/329 (39.82%), Query Frame = 1

Query: 2   ASVHGDQIHSLVWKHGYHAE-------VFVAGALVDMYAKCCDMCMAEKVFEEMP----- 61
           ASVHG+Q+HSLVWKHG+ AE       +F    L+       D      VF         
Sbjct: 145 ASVHGEQMHSLVWKHGFLAENKLYDQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNL 204

Query: 62  ---------VRNLVSWNSMIVGFLQNKLYDQAILFFK-RLLVKSLTALDEVSFSSAL--- 121
                       L  WN + +    + +Y +  LF     L  +  A D V+++  +   
Sbjct: 205 EFGKQVHGVALKLGVWNLVYINNSLSDMYGKCGLFNDVAKLFSNTGARDVVTWNIMIMAY 264

Query: 122 -------SACANV----------DNLEFGKQVHGVALKLGVRNLVYINN----------- 181
                   AC +           D   +   +H  A    +     I+N           
Sbjct: 265 VYNHNYEDACNSFWMMRRKGSIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNL 324

Query: 182 ----SLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRRE 241
               SLI MY+KCG   D  ++F   E R+VV W  +I AC  + +       F  M RE
Sbjct: 325 RVASSLITMYAKCGSLVDAFQIFEETEDRNVVCWTAIIAACQQHGHANWVVELFEQMLRE 384

Query: 242 GLIPDEASYSSVLHSCANLAALYQGTLIHNQIIR-SGFVKNLCVASSLITMYAKCGSLVD 272
           G+ PD  ++ SVL +C++   + +G    N +I+  G        + ++ + ++ G L  
Sbjct: 385 GIKPDYITFVSVLSACSHTGRVEEGFFYFNSMIKVHGIYPGHEHYACIVDLLSRAGELDR 444


HSP 2 Score: 80.9 bits (198), Expect = 5.5e-12
Identity = 73/287 (25.44%), Postives = 127/287 (44.25%), Query Frame = 1

Query: 210 TLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQIFEETQD--RNVVCWTAIITACQ 269
           T IH+Q+I +  +    + ++L+ +YAKCGS+     +F    D  +NVV WT++IT   
Sbjct: 47  TQIHSQLITTALLSLPFLFNNLLNLYAKCGSVDQTLLLFSSAPDDSKNVVSWTSLITQLT 106

Query: 270 QHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGY 329
           +     + +  F  M R G+ P++ TF +VLSAC+ T     G    +S++  HG     
Sbjct: 107 RFKRPFKALTFFNHMRRSGVYPNHYTFSAVLSACTDTTASVHG-EQMHSLVWKHGFLAEN 166

Query: 330 EHYACIVDLLGRAGQLDRAKRFIELMPIKP----DASVWGALLSACRNHSNLEMGKE--- 389
           + Y             D+A  F + + ++     D   + ++ SAC N  NLE GK+   
Sbjct: 167 KLY-------------DQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNLEFGKQVHG 226

Query: 390 VALELFDLEPDNPGNYVLLRNILT-RNGMLNEADEVRRKMETMGVRKEPGCSWIDIKNST 449
           VAL+L         N V + N L+   G     ++V +     G R     +W    N  
Sbjct: 227 VALKL------GVWNLVYINNSLSDMYGKCGLFNDVAKLFSNTGARDV--VTW----NIM 286

Query: 450 FVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAEAYK 487
            +  V + ++E     + M+ +   +  +  Y +      N+A  Y+
Sbjct: 287 IMAYVYNHNYEDACNSFWMMRRKGSIPDEASYSSVLHSCANLAALYQ 307


HSP 3 Score: 65.9 bits (159), Expect = 1.8e-07
Identity = 62/284 (21.83%), Postives = 118/284 (41.55%), Query Frame = 1

Query: 110 QVHGVALKLGVRNLVYINNSLIDMYSKCGLFDDVVKLFSNI--EVRDVVTWNIMIMACVY 169
           Q+H   +   + +L ++ N+L+++Y+KCG  D  + LFS+   + ++VV+W  +I     
Sbjct: 48  QIHSQLITTALLSLPFLFNNLLNLYAKCGSVDQTLLLFSSAPDDSKNVVSWTSLITQLTR 107

Query: 170 NDNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCV 229
                 A   F  MRR G+ P+  ++S+VL +C +  A   G  +H+ + + GF+    +
Sbjct: 108 FKRPFKALTFFNHMRRSGVYPNHYTFSAVLSACTDTTASVHGEQMHSLVWKHGFLAENKL 167

Query: 230 ASSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGI 289
               I  +           + E     + V ++++ +AC   G+     ++    L+ G+
Sbjct: 168 YDQAIFFFKTL--------LLENLTALDEVSFSSVFSACANAGNLEFGKQVHGVALKLGV 227

Query: 290 -KPDYIT------------FVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIV 349
               YI             F  V    S+TG        +N MI  +     YE      
Sbjct: 228 WNLVYINNSLSDMYGKCGLFNDVAKLFSNTG--ARDVVTWNIMIMAYVYNHNYEDACNSF 287

Query: 350 DLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNHSNLEMG 379
            ++ R G +             PD + + ++L +C N + L  G
Sbjct: 288 WMMRRKGSI-------------PDEASYSSVLHSCANLAALYQG 308


HSP 4 Score: 800.0 bits (2065), Expect = 1.8e-228
Identity = 377/558 (67.56%), Postives = 455/558 (81.54%), Query Frame = 1

Query: 4   VHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGF 63
           +HG Q+H L+ KHGY  +VFV  AL DMY KC +M +AEKVF  +P RNLVSWNSMIVG 
Sbjct: 151 LHGQQMHCLISKHGYDTDVFVGSALADMYTKCHNMGLAEKVFVALPERNLVSWNSMIVGC 210

Query: 64  LQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNL 123
           L N L+D+A+L F+ ++ +   + D+VSFSS LSA AN+  LEFGKQ+HG+ +K G+  L
Sbjct: 211 LLNSLHDKALLLFREVIREDFVSPDQVSFSSVLSASANMGALEFGKQIHGMIVKHGLLAL 270

Query: 124 VYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRR 183
            Y+ NSL+DMY KC LFD+   LF+ +  RDV+TWN+M M CVYN+N+E+ACN FW+MRR
Sbjct: 271 SYVKNSLMDMYFKCSLFDEGALLFNTVGARDVITWNVMSMGCVYNENFEEACNYFWVMRR 330

Query: 184 EGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVD 243
            G+ PDEAS S+ LH+ A+LAAL QGTLIHNQII++GF KN C+ASSLITMYAKCGSL D
Sbjct: 331 AGISPDEASCSTALHASAHLAALGQGTLIHNQIIKTGFSKNTCIASSLITMYAKCGSLDD 390

Query: 244 AFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSH 303
           A ++FEE ++RNVVCWTA+I ACQQHG+ N+V++LFE+ML +G+KPDYITFV VLSACSH
Sbjct: 391 ARRVFEEIKNRNVVCWTAMIAACQQHGNGNQVIDLFEKMLADGLKPDYITFVCVLSACSH 450

Query: 304 TGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWG 363
           TGRV+EG+ YFNSM KVHGI PG+EHYAC+VDLLGRAGQLD AK+FI  M IKPD+SVWG
Sbjct: 451 TGRVEEGYAYFNSMEKVHGISPGHEHYACMVDLLGRAGQLDEAKKFIAQMRIKPDSSVWG 510

Query: 364 ALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMG 423
           ALL  C N+ NLE+G EVA  LF+LEP+NPGNYVLL N+    G L EAD+VRR M    
Sbjct: 511 ALLGGCANYGNLELGIEVAGRLFELEPNNPGNYVLLSNMYAHKGKLREADQVRRLMGINR 570

Query: 424 VRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAE 483
           VRKEPGCSWID+KN TFVFTV+DKSH +  EIYEML+K+ ELVK KGYV + ++A+N A+
Sbjct: 571 VRKEPGCSWIDVKNKTFVFTVHDKSHSRMDEIYEMLKKVEELVKDKGYVPQKQYAVNSAD 630

Query: 484 AYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRD 543
             KEQSLWYHSEK+A AFGLL LPAGAPIRIKKNLRTCGDCH VMKFASE+F REII+RD
Sbjct: 631 EDKEQSLWYHSEKIAFAFGLLALPAGAPIRIKKNLRTCGDCHMVMKFASEVFKREIILRD 690

Query: 544 ISRFHHFTNGICSCRDYW 562
           I+RFHHF NG+CSC DYW
Sbjct: 691 INRFHHFRNGLCSCSDYW 708

BLAST of Cla005140 vs. TrEMBL
Match: A0A061F539_THECC (Tetratricopeptide repeat superfamily protein OS=Theobroma cacao GN=TCM_026848 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.8e-21
Identity = 73/287 (25.44%), Postives = 138/287 (48.08%), Query Frame = 1

Query: 93  SSALSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKCGLFDDVVKLFSNIE- 152
           ++ L+  A   +L    Q+H   +     ++ ++ N+L+ +Y+K G     + LFS    
Sbjct: 35  NNLLNTTARTKSLRHAAQIHSQFVTNSFLSVPFLFNNLLSLYAKSGHISHSLLLFSTAHR 94

Query: 153 -VRDVVTWNIMIMACVYNDNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGT 212
             + VV+W  +I      +   +A   F  MR  G+ P+  ++S+VL +CA+   L  G 
Sbjct: 95  VPKGVVSWTTLISHLSRFNTPFEALTLFNHMRSNGVYPNHYTFSAVLPACASTTILLHGQ 154

Query: 213 LIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHG 272
            +H  I + G+  ++ V S+L  MY KC ++  A ++F    +RN+V W ++I  C  + 
Sbjct: 155 QMHCLISKHGYDTDVFVGSALADMYTKCHNMGLAEKVFVALPERNLVSWNSMIVGCLLNS 214

Query: 273 HANRVVELFEQMLREG-IKPDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEH 332
             ++ + LF +++RE  + PD ++F SVLSA ++ G ++ G    + MI  HG+      
Sbjct: 215 LHDKALLLFREVIREDFVSPDQVSFSSVLSASANMGALEFG-KQIHGMIVKHGLLALSYV 274

Query: 333 YACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNHSNLE 377
              ++D+  +    D        +  + D   W  +   C  + N E
Sbjct: 275 KNSLMDMYFKCSLFDEGALLFNTVGAR-DVITWNVMSMGCVYNENFE 319


HSP 2 Score: 787.3 bits (2032), Expect = 1.2e-224
Identity = 378/556 (67.99%), Postives = 451/556 (81.12%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
           G QIH+L++KH +H + FVA AL+ MYA C  M +AE VF++MP RNLVSWNS+IVGF++
Sbjct: 149 GQQIHALLYKHAFHTDTFVATALLHMYATCGSMSLAENVFDQMPHRNLVSWNSLIVGFVK 208

Query: 66  NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVY 125
           NK+Y +AI  F+ +L  SL   D+VSFSS LSACA +  L FGKQVHG  +K G+  LVY
Sbjct: 209 NKMYGRAIGCFREVL--SLDP-DQVSFSSVLSACAGLVELGFGKQVHGSVVKHGLVGLVY 268

Query: 126 INNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREG 185
           + NSL+DMY KCG F+D  KLF     RDVVTWN+MIM CV++ N+E AC  F  M REG
Sbjct: 269 VKNSLVDMYCKCGFFEDATKLFCGGGDRDVVTWNVMIMGCVHSQNFEQACTFFQAMIREG 328

Query: 186 LIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAF 245
           + PDEASYSS+LH+ A+++AL QGTLIH  ++++G +KN CV+SSL+TMY KCGSL DA+
Sbjct: 329 VEPDEASYSSLLHASASISALAQGTLIHCHVLKTGHMKNACVSSSLVTMYGKCGSLFDAY 388

Query: 246 QIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTG 305
           ++F ET+D NVVCWTA+IT C QHG AN  +ELFE+ML+EGI P+YITFVSVLSACSHTG
Sbjct: 389 RVFGETKDCNVVCWTAMITVCHQHGCANEAIELFEEMLKEGIVPEYITFVSVLSACSHTG 448

Query: 306 RVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGAL 365
           +V +GF YFNSM  VH I PG EHYAC+VDLLGR G+L+ A RFIELMPI+PD+SVWGA 
Sbjct: 449 KVDDGFKYFNSMSDVHNIEPGLEHYACMVDLLGRVGRLEEAWRFIELMPIEPDSSVWGAF 508

Query: 366 LSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMGVR 425
           L AC  H N+EMG+EVA  LF+LEPDNPGNYV+L NI TR+GML EADEVRR M   GVR
Sbjct: 509 LGACGKHGNVEMGREVAERLFELEPDNPGNYVVLANIYTRHGMLEEADEVRRLMGINGVR 568

Query: 426 KEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAEAY 485
           KE GCSWID+KN TFVF VND+SH +TQEIYEML+KL+EL+KK+GYVAET+FA N  E  
Sbjct: 569 KETGCSWIDVKNRTFVFNVNDRSHSRTQEIYEMLQKLKELIKKRGYVAETQFATNSVEGS 628

Query: 486 KEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRDIS 545
           +EQSLW HSEKLALAFGLL LP G+P+RIKKNLRTCGDCHTVMKFASEIF REIIVRDI+
Sbjct: 629 EEQSLWCHSEKLALAFGLLVLPPGSPVRIKKNLRTCGDCHTVMKFASEIFQREIIVRDIN 688

Query: 546 RFHHFTNGICSCRDYW 562
           RFH FTNG CSCRDYW
Sbjct: 689 RFHRFTNGSCSCRDYW 701

BLAST of Cla005140 vs. TrEMBL
Match: V7D0V7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G221600g PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.8e-24
Identity = 83/283 (29.33%), Postives = 139/283 (49.12%), Query Frame = 1

Query: 96  LSACANVDNLEFGKQVHGVALKLGVRNLVYINNSLIDMYSKCGLFDDVVKLFSNIE--VR 155
           L+  A + NL+   Q+H   +     +L  IN SLI +Y+KCG     V LF        
Sbjct: 35  LNKAAKLKNLKHATQIHSQIVTTNRTSLGNIN-SLIVVYAKCGSIKHAVLLFGTTPRAST 94

Query: 156 DVVTWNIMIMACVYNDNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGTLIH 215
            VVTW  +I    + +    A +SF +MR  G+ P++ ++S++L +CA+   L QG  IH
Sbjct: 95  SVVTWTTLITQLSHFNKPFQALSSFNLMRTTGIYPNQFTFSAILPACAHATLLSQGQQIH 154

Query: 216 NQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHGHAN 275
             + +  F  +  VA++L+ MYA CGS+  A  +F++   RN+V W ++I    ++    
Sbjct: 155 ALLYKHAFHTDTFVATALLHMYATCGSMSLAENVFDQMPHRNLVSWNSLIVGFVKNKMYG 214

Query: 276 RVVELFEQMLREGIKPDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACI 335
           R +  F ++L   + PD ++F SVLSAC+    +  G     S++K HG+         +
Sbjct: 215 RAIGCFREVL--SLDPDQVSFSSVLSACAGLVELGFGKQVHGSVVK-HGLVGLVYVKNSL 274

Query: 336 VDLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNHSNLE 377
           VD+  + G  + A +         D   W  ++  C +  N E
Sbjct: 275 VDMYCKCGFFEDATKLF-CGGGDRDVVTWNVMIMGCVHSQNFE 312


HSP 2 Score: 785.4 bits (2027), Expect = 4.6e-224
Identity = 374/557 (67.15%), Postives = 446/557 (80.07%), Query Frame = 1

Query: 5   HGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFL 64
           HG QIH+L+ KHG+  + F+A ALVDMYAKC  M +A+ VF++MP RNLVSWNS+IVGFL
Sbjct: 139 HGQQIHALLLKHGFQTDTFIATALVDMYAKCGSMSLAQNVFDQMPHRNLVSWNSIIVGFL 198

Query: 65  QNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLV 124
           +NKLY  AI  FK  + K+    D+V+FSS LSACA +  L  GKQVHG  +K G+  LV
Sbjct: 199 KNKLYGCAIGVFKEFIQKTSLFPDQVTFSSVLSACAGLVALGLGKQVHGSVVKRGLVGLV 258

Query: 125 YINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRRE 184
           Y+ NSL+DMY KCG+F D  KLF +   RDVVTWN++IM CV++ N+E ACN F  M RE
Sbjct: 259 YVKNSLVDMYCKCGVFQDATKLFCDGGDRDVVTWNVIIMGCVHSQNFEQACNFFKDMIRE 318

Query: 185 GLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDA 244
           G+ PDE SYSS+L + A++A L QGTLIH+++ ++G +KN CV SSL+TMY KCGSL DA
Sbjct: 319 GVEPDETSYSSLLQASASIAVLAQGTLIHSRVFKTGHLKNACVLSSLVTMYGKCGSLFDA 378

Query: 245 FQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHT 304
           +++F E +DRNVVCWTA+I  C QHG  N  +ELFE+MLREG+ P+YITFVSVLSACSHT
Sbjct: 379 YRVFGEVKDRNVVCWTAMIAVCHQHGCGNEAIELFEEMLREGVVPEYITFVSVLSACSHT 438

Query: 305 GRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGA 364
           G+V +GF YFNSM  VH I PG EHYAC+ DLLGR G+L+ A RFIE MPI+PD+SVWGA
Sbjct: 439 GKVDDGFKYFNSMANVHHIEPGLEHYACMADLLGRVGRLEEAWRFIESMPIEPDSSVWGA 498

Query: 365 LLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMGV 424
           LL AC  H N+EMG+EVA +LF LEPDNPGNYVLL NI TR+GML EADEVRR M   GV
Sbjct: 499 LLGACGKHGNVEMGREVAEKLFKLEPDNPGNYVLLSNIYTRHGMLEEADEVRRLMGINGV 558

Query: 425 RKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAEA 484
           RKE GCSWID+KN TFVF VND+SH +TQEIYEML+KL+EL+K++GYVAET+FA N  E 
Sbjct: 559 RKETGCSWIDVKNRTFVFNVNDRSHSRTQEIYEMLQKLKELIKRRGYVAETQFATNSVEG 618

Query: 485 YKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRDI 544
            +EQSLW HSEKLALAFGLL LP G+P+RIKKNLRTCGDCHTVMKFASEIF REIIVRDI
Sbjct: 619 SEEQSLWCHSEKLALAFGLLVLPPGSPVRIKKNLRTCGDCHTVMKFASEIFEREIIVRDI 678

Query: 545 SRFHHFTNGICSCRDYW 562
           +RFH FTNG CSCRDYW
Sbjct: 679 NRFHRFTNGSCSCRDYW 695

BLAST of Cla005140 vs. TrEMBL
Match: A0A151TX87_CAJCA (Putative pentatricopeptide repeat-containing protein At3g23330 family OS=Cajanus cajan GN=KK1_010952 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 3.6e-43
Identity = 119/390 (30.51%), Postives = 199/390 (51.03%), Query Frame = 1

Query: 5   HGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMP--VRNLVSWNSMIVG 64
           H  QIHS +      A V    +L+ +YAKC  +     +F   P    N+V+W ++I  
Sbjct: 36  HATQIHSQIVTTN-DASVSNINSLLLVYAKCGSIDHTLLLFATTPHASSNVVTWTTLISQ 95

Query: 65  FLQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRN 124
              +    +A+  F R+   +L   ++ +FS+ L ACA+   L  G+Q+H + LK G + 
Sbjct: 96  LSHSNNPSRALACFNRMRSTTLIYPNQFTFSAILPACAHAPLLSHGQQIHALLLKHGFQT 155

Query: 125 LVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSF-WMM 184
             +I  +L+DMY+KCG       +F  +  R++V+WN +I+  + N  Y  A   F   +
Sbjct: 156 DTFIATALVDMYAKCGSMSLAQNVFDQMPHRNLVSWNSIIVGFLKNKLYGCAIGVFKEFI 215

Query: 185 RREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSL 244
           ++  L PD+ ++SSVL +CA L AL  G  +H  +++ G V  + V +SL+ MY KCG  
Sbjct: 216 QKTSLFPDQVTFSSVLSACAGLVALGLGKQVHGSVVKRGLVGLVYVKNSLVDMYCKCGVF 275

Query: 245 VDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSAC 304
            DA ++F +  DR+VV W  II  C    +  +    F+ M+REG++PD  ++ S+L A 
Sbjct: 276 QDATKLFCDGGDRDVVTWNVIIMGCVHSQNFEQACNFFKDMIREGVEPDETSYSSLLQAS 335

Query: 305 SHTGRVQEGFFYFNSMIKVHGIYPGYEHYACI----VDLLGRAGQLDRAKRFIELMPIKP 364
           +    + +G    + + K      G+   AC+    V + G+ G L  A R    +  + 
Sbjct: 336 ASIAVLAQGTLIHSRVFKT-----GHLKNACVLSSLVTMYGKCGSLFDAYRVFGEVKDR- 395

Query: 365 DASVWGALLSACRNHSNLEMGKEVALELFD 388
           +   W A+++ C  H     G E A+ELF+
Sbjct: 396 NVVCWTAMIAVCHQHG---CGNE-AIELFE 414


HSP 2 Score: 785.4 bits (2027), Expect = 4.6e-224
Identity = 378/556 (67.99%), Postives = 449/556 (80.76%), Query Frame = 1

Query: 6   GDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGFLQ 65
           G QIH+L+ KH +H + FVA AL+ MYA C  M +AE VF++MP RNLVSWNSMIVGFL+
Sbjct: 146 GQQIHALLLKHAFHTDTFVATALLHMYASCGSMSLAENVFDQMPHRNLVSWNSMIVGFLK 205

Query: 66  NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNLVY 125
           NK+Y +AI FF+ +L  SL   D+VSFSS LSACA +  L FGKQVHG  +K G+  LVY
Sbjct: 206 NKMYGRAIGFFREVL--SLEP-DQVSFSSVLSACAGLVELGFGKQVHGSIVKRGLVGLVY 265

Query: 126 INNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRREG 185
           + NSL+DMY KCGLF+D  KLF     RDVVTWN+MI  CV++ N+E AC  F  M REG
Sbjct: 266 VKNSLVDMYCKCGLFEDATKLFCGGGERDVVTWNVMITGCVHSQNFEQACTFFQAMIREG 325

Query: 186 LIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAF 245
           + PDEASYSS+LH+ A++AAL QG+LIH  ++++G +KN CV+SSL+TMY KCGSL DA+
Sbjct: 326 VEPDEASYSSLLHASASIAALAQGSLIHCHVLKTGHMKNACVSSSLVTMYGKCGSLFDAY 385

Query: 246 QIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTG 305
            +F ET+D NVVCWTA+IT C QHG AN  +ELFE+ML+EGI P+YITFVSVLSACSHTG
Sbjct: 386 GVFGETKDCNVVCWTAMITVCHQHGCANEAIELFEEMLKEGIVPEYITFVSVLSACSHTG 445

Query: 306 RVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWGAL 365
           +V +GF YFNSM  VH I PG EHYAC+VDLLGR G+L+ A RFIELMPI+PD++VWGA 
Sbjct: 446 KVDDGFKYFNSMANVHNIEPGLEHYACMVDLLGRVGRLEEAWRFIELMPIEPDSTVWGAF 505

Query: 366 LSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMGVR 425
           L AC  H N+EMG+EVA  LF LEPDNPGNY LL NI TR+GML+EADEVRR M   GVR
Sbjct: 506 LGACGKHGNVEMGREVAERLFKLEPDNPGNYALLANIYTRHGMLDEADEVRRLMGVSGVR 565

Query: 426 KEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAEAY 485
           KE GCSWID+KN TFVF VND+SH +T+EIYEML+KL+EL+K++GYVAET+FA N  E  
Sbjct: 566 KETGCSWIDVKNRTFVFNVNDRSHSRTREIYEMLQKLKELMKRRGYVAETQFATNSVEGS 625

Query: 486 KEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRDIS 545
           +EQSLW HSEKLALAFGLL LP G+P+RIKKNLRTCGDCHTVMKFASEIF REIIVRDI+
Sbjct: 626 EEQSLWCHSEKLALAFGLLVLPPGSPVRIKKNLRTCGDCHTVMKFASEIFQREIIVRDIN 685

Query: 546 RFHHFTNGICSCRDYW 562
           RFH FTNG CSCRDYW
Sbjct: 686 RFHRFTNGSCSCRDYW 698

BLAST of Cla005140 vs. NCBI nr
Match: gi|659099256|ref|XP_008450508.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-like [Cucumis melo])

HSP 1 Score: 1043.1 bits (2696), Expect = 1.7e-301
Identity = 504/561 (89.84%), Postives = 534/561 (95.19%), Query Frame = 1

Query: 1   MASVHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMI 60
           M SVHG+Q+HSLVWKHG+HAEVFV  ALVDMYAKCCDM MAEKVFEEMPVRNLVSWNSMI
Sbjct: 144 MVSVHGEQMHSLVWKHGFHAEVFVVSALVDMYAKCCDMRMAEKVFEEMPVRNLVSWNSMI 203

Query: 61  VGFLQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGV 120
           VGFLQNKLYDQAI FFK LL+++LTALDEVSFSS  SACAN  NL FGKQVHGVALKLGV
Sbjct: 204 VGFLQNKLYDQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNLAFGKQVHGVALKLGV 263

Query: 121 RNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWM 180
           RNLVYINNSLIDMYSKCGLF++V KLFSNI  RDVVTWNIMIMACV+N NYEDACNSFWM
Sbjct: 264 RNLVYINNSLIDMYSKCGLFNEVAKLFSNIGARDVVTWNIMIMACVHNHNYEDACNSFWM 323

Query: 181 MRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGS 240
           MR++G IPDEASYSSVLHSCANLA LYQGTLIHNQIIRSGFVKNLCVASSLI MYAKCGS
Sbjct: 324 MRKKGSIPDEASYSSVLHSCANLATLYQGTLIHNQIIRSGFVKNLCVASSLIKMYAKCGS 383

Query: 241 LVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA 300
           LVDAFQIFEE +DRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA
Sbjct: 384 LVDAFQIFEEIEDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA 443

Query: 301 CSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDAS 360
           CSHTGRV+EGFFYFNSMIK+HGI+PG+EHYACIVDLL RAG+L+RAKRFIELMPIKPDAS
Sbjct: 444 CSHTGRVEEGFFYFNSMIKLHGIFPGHEHYACIVDLLSRAGELNRAKRFIELMPIKPDAS 503

Query: 361 VWGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKME 420
           VWGALLSACRNHSNL MGKEVAL+LF+LEPDNPGNYVLL NILTRNGMLNEAD+VRRKME
Sbjct: 504 VWGALLSACRNHSNLVMGKEVALKLFELEPDNPGNYVLLCNILTRNGMLNEADKVRRKME 563

Query: 421 TMGVRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAIN 480
            +GVRKEPGCSWID+KNST+VFTV+DKSHEKT+EIYEMLE L+ELVKKKGYVAETEFAIN
Sbjct: 564 RIGVRKEPGCSWIDVKNSTYVFTVHDKSHEKTKEIYEMLENLKELVKKKGYVAETEFAIN 623

Query: 481 IAEAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII 540
            AE YKEQSLWYH+EKLALAFGLL+LPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII
Sbjct: 624 TAEEYKEQSLWYHTEKLALAFGLLSLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII 683

Query: 541 VRDISRFHHFTNGICSCRDYW 562
           VRDI+RFHHFTNGICSC DYW
Sbjct: 684 VRDINRFHHFTNGICSCGDYW 704

BLAST of Cla005140 vs. NCBI nr
Match: gi|659099256|ref|XP_008450508.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-like [Cucumis melo])

HSP 1 Score: 164.9 bits (416), Expect = 4.2e-37
Identity = 109/388 (28.09%), Postives = 194/388 (50.00%), Query Frame = 1

Query: 3   SVHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMP--VRNLVSWNSMI 62
           S H  QIHS +      +  F+   L+++YAKC  +     +F   P   +++VSW S+I
Sbjct: 43  SKHATQIHSQLITTALLSLPFLFNNLLNLYAKCGCVDQTLLLFSSAPDVSKDVVSWTSLI 102

Query: 63  VGFLQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGV 122
               ++K   +A+ FF ++ +  +   +  + S+ LSAC +      G+Q+H +  K G 
Sbjct: 103 TQLTRSKRPFKALTFFNQMRLSGVYP-NHYTLSAVLSACTDTMVSVHGEQMHSLVWKHGF 162

Query: 123 RNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWM 182
              V++ ++L+DMY+KC       K+F  + VR++V+WN MI+  + N  Y+ A   F  
Sbjct: 163 HAEVFVVSALVDMYAKCCDMRMAEKVFEEMPVRNLVSWNSMIVGFLQNKLYDQAIFFFKT 222

Query: 183 MRREGLIP-DEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCG 242
           +  E L   DE S+SSV  +CAN   L  G  +H   ++ G    + + +SLI MY+KCG
Sbjct: 223 LLLENLTALDEVSFSSVFSACANAGNLAFGKQVHGVALKLGVRNLVYINNSLIDMYSKCG 282

Query: 243 SLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLS 302
              +  ++F     R+VV W  +I AC  + +       F  M ++G  PD  ++ SVL 
Sbjct: 283 LFNEVAKLFSNIGARDVVTWNIMIMACVHNHNYEDACNSFWMMRKKGSIPDEASYSSVLH 342

Query: 303 ACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDA 362
           +C++   + +G    N +I+  G        + ++ +  + G L  A +  E +  + + 
Sbjct: 343 SCANLATLYQGTLIHNQIIR-SGFVKNLCVASSLIKMYAKCGSLVDAFQIFEEIEDR-NV 402

Query: 363 SVWGALLSACRNHSNLEMGKEVALELFD 388
             W A+++AC+ H +        +ELF+
Sbjct: 403 VCWTAIITACQQHGHANR----VVELFE 423


HSP 2 Score: 1039.3 bits (2686), Expect = 2.5e-300
Identity = 508/560 (90.71%), Postives = 531/560 (94.82%), Query Frame = 1

Query: 2   ASVHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIV 61
           ASVHG+Q+HSLVWKHG+ AEVFV  ALVDMYAKCCDM MAEKVFEEMPVRNLVSWN+MIV
Sbjct: 145 ASVHGEQMHSLVWKHGFLAEVFVVSALVDMYAKCCDMLMAEKVFEEMPVRNLVSWNTMIV 204

Query: 62  GFLQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVR 121
           GFLQNKLYDQAI FFK LL+++LTALDEVSFSS  SACAN  NLEFGKQVHGVALKLGV 
Sbjct: 205 GFLQNKLYDQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNLEFGKQVHGVALKLGVW 264

Query: 122 NLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMM 181
           NLVYINNSL DMY KCGLF+DV KLFSN   RDVVTWNIMIMA VYN NYEDACNSFWMM
Sbjct: 265 NLVYINNSLSDMYGKCGLFNDVAKLFSNTGARDVVTWNIMIMAYVYNHNYEDACNSFWMM 324

Query: 182 RREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSL 241
           RR+G IPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNL VASSLITMYAKCGSL
Sbjct: 325 RRKGSIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLRVASSLITMYAKCGSL 384

Query: 242 VDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSAC 301
           VDAFQIFEET+DRNVVCWTAII ACQQHGHAN VVELFEQMLREGIKPDYITFVSVLSAC
Sbjct: 385 VDAFQIFEETEDRNVVCWTAIIAACQQHGHANWVVELFEQMLREGIKPDYITFVSVLSAC 444

Query: 302 SHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASV 361
           SHTGRV+EGFFYFNSMIKVHGIYPG+EHYACIVDLL RAG+LDRAKRFIELMPIKPDASV
Sbjct: 445 SHTGRVEEGFFYFNSMIKVHGIYPGHEHYACIVDLLSRAGELDRAKRFIELMPIKPDASV 504

Query: 362 WGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMET 421
           WGALLSACRNHSNL MGKEVAL+LFDLEPDNPGNYVLL NILTRNGMLNEADEVRRKME+
Sbjct: 505 WGALLSACRNHSNLIMGKEVALKLFDLEPDNPGNYVLLCNILTRNGMLNEADEVRRKMES 564

Query: 422 MGVRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINI 481
           +GVRKEPGCSWIDIKNST+VFTV+DKSHEKT+EIYEMLEKL+ELVKKKGYVAETEFAIN 
Sbjct: 565 IGVRKEPGCSWIDIKNSTYVFTVHDKSHEKTKEIYEMLEKLKELVKKKGYVAETEFAINT 624

Query: 482 AEAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIV 541
           AE YKEQSLWYHSEK+ALAFGLL+LPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIV
Sbjct: 625 AEEYKEQSLWYHSEKIALAFGLLSLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIV 684

Query: 542 RDISRFHHFTNGICSCRDYW 562
           RDI+RFHHFTNGICSC DYW
Sbjct: 685 RDINRFHHFTNGICSCGDYW 704

BLAST of Cla005140 vs. NCBI nr
Match: gi|778662180|ref|XP_011659482.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 166.0 bits (419), Expect = 1.9e-37
Identity = 113/388 (29.12%), Postives = 190/388 (48.97%), Query Frame = 1

Query: 3   SVHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMP--VRNLVSWNSMI 62
           S H  QIHS +      +  F+   L+++YAKC  +     +F   P   +N+VSW S+I
Sbjct: 43  SKHATQIHSQLITTALLSLPFLFNNLLNLYAKCGSVDQTLLLFSSAPDDSKNVVSWTSLI 102

Query: 63  VGFLQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGV 122
               + K   +A+ FF  +  +S    +  +FS+ LSAC +      G+Q+H +  K G 
Sbjct: 103 TQLTRFKRPFKALTFFNHMR-RSGVYPNHYTFSAVLSACTDTTASVHGEQMHSLVWKHGF 162

Query: 123 RNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWM 182
              V++ ++L+DMY+KC       K+F  + VR++V+WN MI+  + N  Y+ A   F  
Sbjct: 163 LAEVFVVSALVDMYAKCCDMLMAEKVFEEMPVRNLVSWNTMIVGFLQNKLYDQAIFFFKT 222

Query: 183 MRREGLIP-DEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCG 242
           +  E L   DE S+SSV  +CAN   L  G  +H   ++ G    + + +SL  MY KCG
Sbjct: 223 LLLENLTALDEVSFSSVFSACANAGNLEFGKQVHGVALKLGVWNLVYINNSLSDMYGKCG 282

Query: 243 SLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLS 302
              D  ++F  T  R+VV W  +I A   + +       F  M R+G  PD  ++ SVL 
Sbjct: 283 LFNDVAKLFSNTGARDVVTWNIMIMAYVYNHNYEDACNSFWMMRRKGSIPDEASYSSVLH 342

Query: 303 ACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDA 362
           +C++   + +G    N +I+  G        + ++ +  + G L  A +  E    + + 
Sbjct: 343 SCANLAALYQGTLIHNQIIR-SGFVKNLRVASSLITMYAKCGSLVDAFQIFEETEDR-NV 402

Query: 363 SVWGALLSACRNHSNLEMGKEVALELFD 388
             W A+++AC+ H +        +ELF+
Sbjct: 403 VCWTAIIAACQQHGHANW----VVELFE 423


HSP 2 Score: 924.5 bits (2388), Expect = 9.0e-266
Identity = 455/501 (90.82%), Postives = 475/501 (94.81%), Query Frame = 1

Query: 62  GFL-QNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGV 121
           GFL +NKLYDQAI FFK LL+++LTALDEVSFSS  SACAN  NLEFGKQVHGVALKLGV
Sbjct: 160 GFLAENKLYDQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNLEFGKQVHGVALKLGV 219

Query: 122 RNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWM 181
            NLVYINNSL DMY KCGLF+DV KLFSN   RDVVTWNIMIMA VYN NYEDACNSFWM
Sbjct: 220 WNLVYINNSLSDMYGKCGLFNDVAKLFSNTGARDVVTWNIMIMAYVYNHNYEDACNSFWM 279

Query: 182 MRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGS 241
           MRR+G IPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNL VASSLITMYAKCGS
Sbjct: 280 MRRKGSIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLRVASSLITMYAKCGS 339

Query: 242 LVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA 301
           LVDAFQIFEET+DRNVVCWTAII ACQQHGHAN VVELFEQMLREGIKPDYITFVSVLSA
Sbjct: 340 LVDAFQIFEETEDRNVVCWTAIIAACQQHGHANWVVELFEQMLREGIKPDYITFVSVLSA 399

Query: 302 CSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDAS 361
           CSHTGRV+EGFFYFNSMIKVHGIYPG+EHYACIVDLL RAG+LDRAKRFIELMPIKPDAS
Sbjct: 400 CSHTGRVEEGFFYFNSMIKVHGIYPGHEHYACIVDLLSRAGELDRAKRFIELMPIKPDAS 459

Query: 362 VWGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKME 421
           VWGALLSACRNHSNL MGKEVAL+LFDLEPDNPGNYVLL NILTRNGMLNEADEVRRKME
Sbjct: 460 VWGALLSACRNHSNLIMGKEVALKLFDLEPDNPGNYVLLCNILTRNGMLNEADEVRRKME 519

Query: 422 TMGVRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAIN 481
           ++GVRKEPGCSWIDIKNST+VFTV+DKSHEKT+EIYEMLEKL+ELVKKKGYVAETEFAIN
Sbjct: 520 SIGVRKEPGCSWIDIKNSTYVFTVHDKSHEKTKEIYEMLEKLKELVKKKGYVAETEFAIN 579

Query: 482 IAEAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII 541
            AE YKEQSLWYHSEK+ALAFGLL+LPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII
Sbjct: 580 TAEEYKEQSLWYHSEKIALAFGLLSLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII 639

Query: 542 VRDISRFHHFTNGICSCRDYW 562
           VRDI+RFHHFTNGICSC DYW
Sbjct: 640 VRDINRFHHFTNGICSCGDYW 660

BLAST of Cla005140 vs. NCBI nr
Match: gi|700210899|gb|KGN65995.1| (hypothetical protein Csa_1G560780 [Cucumis sativus])

HSP 1 Score: 83.6 bits (205), Expect = 1.2e-12
Identity = 80/329 (24.32%), Postives = 131/329 (39.82%), Query Frame = 1

Query: 2   ASVHGDQIHSLVWKHGYHAE-------VFVAGALVDMYAKCCDMCMAEKVFEEMP----- 61
           ASVHG+Q+HSLVWKHG+ AE       +F    L+       D      VF         
Sbjct: 145 ASVHGEQMHSLVWKHGFLAENKLYDQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNL 204

Query: 62  ---------VRNLVSWNSMIVGFLQNKLYDQAILFFK-RLLVKSLTALDEVSFSSAL--- 121
                       L  WN + +    + +Y +  LF     L  +  A D V+++  +   
Sbjct: 205 EFGKQVHGVALKLGVWNLVYINNSLSDMYGKCGLFNDVAKLFSNTGARDVVTWNIMIMAY 264

Query: 122 -------SACANV----------DNLEFGKQVHGVALKLGVRNLVYINN----------- 181
                   AC +           D   +   +H  A    +     I+N           
Sbjct: 265 VYNHNYEDACNSFWMMRRKGSIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNL 324

Query: 182 ----SLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRRE 241
               SLI MY+KCG   D  ++F   E R+VV W  +I AC  + +       F  M RE
Sbjct: 325 RVASSLITMYAKCGSLVDAFQIFEETEDRNVVCWTAIIAACQQHGHANWVVELFEQMLRE 384

Query: 242 GLIPDEASYSSVLHSCANLAALYQGTLIHNQIIR-SGFVKNLCVASSLITMYAKCGSLVD 272
           G+ PD  ++ SVL +C++   + +G    N +I+  G        + ++ + ++ G L  
Sbjct: 385 GIKPDYITFVSVLSACSHTGRVEEGFFYFNSMIKVHGIYPGHEHYACIVDLLSRAGELDR 444


HSP 2 Score: 80.9 bits (198), Expect = 8.0e-12
Identity = 73/287 (25.44%), Postives = 127/287 (44.25%), Query Frame = 1

Query: 210 TLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVDAFQIFEETQD--RNVVCWTAIITACQ 269
           T IH+Q+I +  +    + ++L+ +YAKCGS+     +F    D  +NVV WT++IT   
Sbjct: 47  TQIHSQLITTALLSLPFLFNNLLNLYAKCGSVDQTLLLFSSAPDDSKNVVSWTSLITQLT 106

Query: 270 QHGHANRVVELFEQMLREGIKPDYITFVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGY 329
           +     + +  F  M R G+ P++ TF +VLSAC+ T     G    +S++  HG     
Sbjct: 107 RFKRPFKALTFFNHMRRSGVYPNHYTFSAVLSACTDTTASVHG-EQMHSLVWKHGFLAEN 166

Query: 330 EHYACIVDLLGRAGQLDRAKRFIELMPIKP----DASVWGALLSACRNHSNLEMGKE--- 389
           + Y             D+A  F + + ++     D   + ++ SAC N  NLE GK+   
Sbjct: 167 KLY-------------DQAIFFFKTLLLENLTALDEVSFSSVFSACANAGNLEFGKQVHG 226

Query: 390 VALELFDLEPDNPGNYVLLRNILT-RNGMLNEADEVRRKMETMGVRKEPGCSWIDIKNST 449
           VAL+L         N V + N L+   G     ++V +     G R     +W    N  
Sbjct: 227 VALKL------GVWNLVYINNSLSDMYGKCGLFNDVAKLFSNTGARDV--VTW----NIM 286

Query: 450 FVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAEAYK 487
            +  V + ++E     + M+ +   +  +  Y +      N+A  Y+
Sbjct: 287 IMAYVYNHNYEDACNSFWMMRRKGSIPDEASYSSVLHSCANLAALYQ 307


HSP 3 Score: 65.9 bits (159), Expect = 2.6e-07
Identity = 62/284 (21.83%), Postives = 118/284 (41.55%), Query Frame = 1

Query: 110 QVHGVALKLGVRNLVYINNSLIDMYSKCGLFDDVVKLFSNI--EVRDVVTWNIMIMACVY 169
           Q+H   +   + +L ++ N+L+++Y+KCG  D  + LFS+   + ++VV+W  +I     
Sbjct: 48  QIHSQLITTALLSLPFLFNNLLNLYAKCGSVDQTLLLFSSAPDDSKNVVSWTSLITQLTR 107

Query: 170 NDNYEDACNSFWMMRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCV 229
                 A   F  MRR G+ P+  ++S+VL +C +  A   G  +H+ + + GF+    +
Sbjct: 108 FKRPFKALTFFNHMRRSGVYPNHYTFSAVLSACTDTTASVHGEQMHSLVWKHGFLAENKL 167

Query: 230 ASSLITMYAKCGSLVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGI 289
               I  +           + E     + V ++++ +AC   G+     ++    L+ G+
Sbjct: 168 YDQAIFFFKTL--------LLENLTALDEVSFSSVFSACANAGNLEFGKQVHGVALKLGV 227

Query: 290 -KPDYIT------------FVSVLSACSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIV 349
               YI             F  V    S+TG        +N MI  +     YE      
Sbjct: 228 WNLVYINNSLSDMYGKCGLFNDVAKLFSNTG--ARDVVTWNIMIMAYVYNHNYEDACNSF 287

Query: 350 DLLGRAGQLDRAKRFIELMPIKPDASVWGALLSACRNHSNLEMG 379
            ++ R G +             PD + + ++L +C N + L  G
Sbjct: 288 WMMRRKGSI-------------PDEASYSSVLHSCANLAALYQG 308


HSP 4 Score: 835.5 bits (2157), Expect = 5.5e-239
Identity = 395/558 (70.79%), Postives = 467/558 (83.69%), Query Frame = 1

Query: 4   VHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMIVGF 63
           VHG QIHSL+ KHG+  +VFV  A++DMYAKC +M  AEKVF+EMP RNLVSWN+MIVGF
Sbjct: 161 VHGQQIHSLIHKHGFGTDVFVGSAMIDMYAKCSNMDSAEKVFDEMPERNLVSWNAMIVGF 220

Query: 64  LQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVRNL 123
             NK++D+AI  FK +L     + D+VSFSS LSACANV +L+FG+QVHGV +K G+ +L
Sbjct: 221 SHNKIFDRAIDVFKEVLRDKSVSPDQVSFSSVLSACANVGSLDFGRQVHGVVVKHGLMHL 280

Query: 124 VYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWMMRR 183
            Y+ NSL+DMY+KCG F D ++LF +I  RDVVTWN+M M CV ND +E+ACN FW+MRR
Sbjct: 281 AYVKNSLVDMYNKCGCFQDSIQLFGSIPDRDVVTWNVMAMGCVQNDRFEEACNYFWVMRR 340

Query: 184 EGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGSLVD 243
           EG+ PDEAS+S+ LH+ ANLAAL QGTLIH+QII++G++KN CV SSLITMYAKCGSL+D
Sbjct: 341 EGISPDEASFSTALHASANLAALDQGTLIHDQIIKTGYMKNTCVGSSLITMYAKCGSLID 400

Query: 244 AFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSACSH 303
           A ++FEE +DRNVVCWTA+I ACQQHG    V+ELFE+ML EGIKPDYITFV VLSACSH
Sbjct: 401 AHRVFEEIEDRNVVCWTAMIAACQQHGCGGSVIELFEEMLAEGIKPDYITFVCVLSACSH 460

Query: 304 TGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDASVWG 363
           TGRV+EGF YF SM +VHG+ PG EHYAC+VD+LGR+G+LD AKRFIE MPI+PD SVWG
Sbjct: 461 TGRVEEGFGYFESMGRVHGMKPGPEHYACMVDMLGRSGRLDEAKRFIESMPIRPDPSVWG 520

Query: 364 ALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKMETMG 423
           ALL ACRN+  L+MGKEVA +LF++EP+NPGNYVLL NI T  G L EA E+RR M   G
Sbjct: 521 ALLGACRNYGYLDMGKEVAEKLFEMEPNNPGNYVLLSNIYTHYGKLEEAKEIRRLMGING 580

Query: 424 VRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAINIAE 483
            RKEPGCSWIDIKN+T VFTV+DKSH +T EIYEML K+ ELVKKKGYVAET+FA+N  E
Sbjct: 581 ARKEPGCSWIDIKNTTHVFTVHDKSHSRTDEIYEMLSKMIELVKKKGYVAETQFAVNDVE 640

Query: 484 AYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREIIVRD 543
           +YKEQSLWYHSEKLALAFGLL+LPAGAPIRIKKNLRTCGDCHTVMK ASEIF REI+VRD
Sbjct: 641 SYKEQSLWYHSEKLALAFGLLSLPAGAPIRIKKNLRTCGDCHTVMKLASEIFEREIVVRD 700

Query: 544 ISRFHHFTNGICSCRDYW 562
           I+RFH F +G CSC DYW
Sbjct: 701 INRFHRFVSGSCSCGDYW 718

BLAST of Cla005140 vs. NCBI nr
Match: gi|720076691|ref|XP_010240804.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At3g13770, mitochondrial [Nelumbo nucifera])

HSP 1 Score: 189.9 bits (481), Expect = 1.2e-44
Identity = 124/431 (28.77%), Postives = 219/431 (50.81%), Query Frame = 1

Query: 5   HGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEM--PVRNLVSWNSMIVG 64
           H  QIH+ +  + Y +  F+   L+++YA    +  +  VF  +    +N+V+W S+I  
Sbjct: 59  HATQIHAQIIINNYSSLTFLFNNLINLYAISGHINQSVLVFSNIHHDTKNIVTWTSLIAQ 118

Query: 65  FLQ-NKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGVR 124
               +K ++   LF +  +  S T  +  +FS+ LSACA+   +  G+Q+H +  K G  
Sbjct: 119 LSHAHKPFESLALFNQ--MGSSGTRPNHFTFSAILSACADTTIIVHGQQIHSLIHKHGFG 178

Query: 125 NLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSF-WM 184
             V++ +++IDMY+KC   D   K+F  +  R++V+WN MI+   +N  ++ A + F  +
Sbjct: 179 TDVFVGSAMIDMYAKCSNMDSAEKVFDEMPERNLVSWNAMIVGFSHNKIFDRAIDVFKEV 238

Query: 185 MRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGS 244
           +R + + PD+ S+SSVL +CAN+ +L  G  +H  +++ G +    V +SL+ MY KCG 
Sbjct: 239 LRDKSVSPDQVSFSSVLSACANVGSLDFGRQVHGVVVKHGLMHLAYVKNSLVDMYNKCGC 298

Query: 245 LVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA 304
             D+ Q+F    DR+VV W  +   C Q+         F  M REGI PD  +F + L A
Sbjct: 299 FQDSIQLFGSIPDRDVVTWNVMAMGCVQNDRFEEACNYFWVMRREGISPDEASFSTALHA 358

Query: 305 CSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACI----VDLLGRAGQLDRAKRFIELMPIK 364
            ++   + +G    + +IK      GY    C+    + +  + G L  A R  E +  +
Sbjct: 359 SANLAALDQGTLIHDQIIKT-----GYMKNTCVGSSLITMYAKCGSLIDAHRVFEEIEDR 418

Query: 365 PDASVWGALLSACRNH----SNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEA 424
            +   W A+++AC+ H    S +E+ +E+  E   ++PD    Y+    +L+        
Sbjct: 419 -NVVCWTAMIAACQQHGCGGSVIELFEEMLAE--GIKPD----YITFVCVLSACSHTGRV 475


HSP 2 Score: 814.7 bits (2103), Expect = 1.0e-232
Identity = 383/561 (68.27%), Postives = 462/561 (82.35%), Query Frame = 1

Query: 1   MASVHGDQIHSLVWKHGYHAEVFVAGALVDMYAKCCDMCMAEKVFEEMPVRNLVSWNSMI 60
           M   HG+Q+H L+WKHG+ A VFV  ALVDMYAK  +M +AE+VF+EMP RNLVSWNSMI
Sbjct: 152 MIVFHGEQLHCLIWKHGFDAHVFVCSALVDMYAKSSEMTLAERVFDEMPERNLVSWNSMI 211

Query: 61  VGFLQNKLYDQAILFFKRLLVKSLTALDEVSFSSALSACANVDNLEFGKQVHGVALKLGV 120
           VGFLQN+LYDQA+ FF+ +  +S  + D+VSFSS LSACAN+  LEFG+QVHG+  K G+
Sbjct: 212 VGFLQNELYDQAVEFFREVHRESEVSPDQVSFSSVLSACANMGALEFGRQVHGIGFKQGL 271

Query: 121 RNLVYINNSLIDMYSKCGLFDDVVKLFSNIEVRDVVTWNIMIMACVYNDNYEDACNSFWM 180
             L Y+ NSL+DMY KCG   D   LF  I  RDVV+WN+M++ C+ N  +EDACN FW+
Sbjct: 272 VTLTYVKNSLVDMYCKCGWLADAANLFWTIGERDVVSWNVMVIGCICNHKFEDACNYFWV 331

Query: 181 MRREGLIPDEASYSSVLHSCANLAALYQGTLIHNQIIRSGFVKNLCVASSLITMYAKCGS 240
           MRREG+ PDE SY SVL++ A+LAAL QGTLIH+QII+SGF K+ CV+SSLITMYAKCGS
Sbjct: 332 MRREGITPDEVSYCSVLNASASLAALDQGTLIHDQIIKSGFAKSACVSSSLITMYAKCGS 391

Query: 241 LVDAFQIFEETQDRNVVCWTAIITACQQHGHANRVVELFEQMLREGIKPDYITFVSVLSA 300
           L+DA+++F ET +RNVVCWTA++ A QQHG AN+V+ELFE+ML E +KP+Y+T+VS LSA
Sbjct: 392 LIDAYRMFNETDERNVVCWTAMLAAYQQHGCANQVIELFEKMLEEEMKPNYVTYVSALSA 451

Query: 301 CSHTGRVQEGFFYFNSMIKVHGIYPGYEHYACIVDLLGRAGQLDRAKRFIELMPIKPDAS 360
           CSHTGR+++GF YF+SM KVHGI PG EHYAC+VDLLGRAG+LD A+RFIE +P+KP+AS
Sbjct: 452 CSHTGRIEKGFKYFDSMTKVHGINPGREHYACMVDLLGRAGRLDEARRFIETIPVKPNAS 511

Query: 361 VWGALLSACRNHSNLEMGKEVALELFDLEPDNPGNYVLLRNILTRNGMLNEADEVRRKME 420
           +WGALL AC  + N++MG+EVA  LF+LEP+NPGNY+LL N+ TR G L EADEVRR M 
Sbjct: 512 IWGALLGACMKYDNIKMGREVAERLFELEPNNPGNYLLLSNMYTRKGRLEEADEVRRLMG 571

Query: 421 TMGVRKEPGCSWIDIKNSTFVFTVNDKSHEKTQEIYEMLEKLRELVKKKGYVAETEFAIN 480
             GVRKEPGCSWID+KN TFVFTV+D+S+ +  +IYEML KLREL+KKKGYVAET+FA N
Sbjct: 572 VNGVRKEPGCSWIDVKNRTFVFTVHDRSNSRNDKIYEMLRKLRELMKKKGYVAETQFANN 631

Query: 481 IAEAYKEQSLWYHSEKLALAFGLLTLPAGAPIRIKKNLRTCGDCHTVMKFASEIFAREII 540
                KEQ LWYHSEKLALAFGLLTLPA APIRIKKNLRTCGDCHT MKFASEIF REII
Sbjct: 632 SMVKSKEQDLWYHSEKLALAFGLLTLPAKAPIRIKKNLRTCGDCHTFMKFASEIFLREII 691

Query: 541 VRDISRFHHFTNGICSCRDYW 562
           VRD++RFH FTNG CSC DYW
Sbjct: 692 VRDVNRFHRFTNGFCSCGDYW 712

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP252_ARATH2.2e-13142.75Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidop... [more]
PP168_ARATH7.9e-12941.00Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana GN... [more]
PP251_ARATH9.7e-12738.79Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis th... [more]
PP307_ARATH1.7e-12640.93Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN... [more]
PPR32_ARATH2.2e-12642.27Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A0A0LVV0_CUCSA6.3e-26690.82Uncharacterized protein OS=Cucumis sativus GN=Csa_1G560780 PE=4 SV=1[more]
A0A0A0LVV0_CUCSA8.5e-1324.32Uncharacterized protein OS=Cucumis sativus GN=Csa_1G560780 PE=4 SV=1[more]
A0A061F539_THECC3.8e-2125.44Tetratricopeptide repeat superfamily protein OS=Theobroma cacao GN=TCM_026848 PE... [more]
V7D0V7_PHAVU2.8e-2429.33Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G221600g PE=4 SV=1[more]
A0A151TX87_CAJCA3.6e-4330.51Putative pentatricopeptide repeat-containing protein At3g23330 family OS=Cajanus... [more]
Match NameE-valueIdentityDescription
gi|659099256|ref|XP_008450508.1|1.7e-30189.84PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-... [more]
gi|659099256|ref|XP_008450508.1|4.2e-3728.09PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-... [more]
gi|778662180|ref|XP_011659482.1|1.9e-3729.12PREDICTED: pentatricopeptide repeat-containing protein At3g24000, mitochondrial-... [more]
gi|700210899|gb|KGN65995.1|1.2e-1224.32hypothetical protein Csa_1G560780 [Cucumis sativus][more]
gi|720076691|ref|XP_010240804.1|1.2e-4428.77PREDICTED: putative pentatricopeptide repeat-containing protein At3g13770, mitoc... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
biological_process GO:0009451 RNA modification
cellular_component GO:0005575 cellular_component
cellular_component GO:0005739 mitochondrion
molecular_function GO:0008568 microtubule-severing ATPase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla005140Cla005140.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 27..52
score: 1.2coord: 402..424
score: 0.31coord: 128..151
score: 0.0025coord: 54..81
score: 0.
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 255..302
score: 3.8E-10coord: 154..201
score: 7.0
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 156..190
score: 0.0017coord: 257..290
score: 3.5E-6coord: 292..325
score: 0.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 290..325
score: 8.221coord: 358..388
score: 5.514coord: 392..426
score: 8.122coord: 52..86
score: 8.868coord: 154..188
score: 10.753coord: 326..356
score: 6.314coord: 123..153
score: 6.807coord: 224..254
score: 7.366coord: 189..223
score: 7.037coord: 88..122
score: 6.686coord: 21..51
score: 6.401coord: 255..289
score: 1
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 244..417
score: 4.9E-7coord: 155..176
score: 4.
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 5..433
score: 7.2E