Cla004545 (gene) Watermelon (97103) v1

BLAST of Cla004545 vs. Swiss-Prot
Match: MSL2_ARATH (Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana GN=MSL2 PE=2 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 9.1e-190
Identity = 360/592 (60.81%), Postives = 422/592 (71.28%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           NDN WKKS TY++MT Y+QPLLLW GA  ICRALDPVVLPTE+S++VK R+LNFVRSLST
Sbjct: 142 NDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVLPTEASKIVKDRLLNFVRSLST 201

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLAFAYCLSS+IQQ QK FSE++  SD RNMGFQFA KA+YSAVWVAA+SLFMELLGFST
Sbjct: 202 VLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKALYSAVWVAAVSLFMELLGFST 261

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPFV+NEWIQTKIEGYEVSGTVEHVG
Sbjct: 262 QKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVLNEWIQTKIEGYEVSGTVEHVG 321

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTIIRGEDREA+HIPNHKFTVNVVRNL+QKTHWRIKTHLAISHLDVNKINNIVADMR
Sbjct: 322 WWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAISHLDVNKINNIVADMR 381

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVKTSH EEYL VKEAI+LDLLRV
Sbjct: 382 KVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHEEYLGVKEAILLDLLRV 441

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           I HHRARLATPIRT++KM++++D+E+ PF +S++G  G T  R +++IEP YK+ GED+ 
Sbjct: 442 ISHHRARLATPIRTIRKMYTETDVENTPFGESMYG--GVTSRRPLMLIEPAYKINGEDKS 501

Query: 404 QSHSRTSRTTGEQNGKPVAR--SSGDSKAAKETMSSDRKTEVKTGGDADTKMHPKVSMST 463
           +S          +  KP A   + G +  +KET S D K  VK G               
Sbjct: 502 KSQ--------NRAAKPTAEQENKGSNPKSKETSSPDLKANVKVG--------------- 561

Query: 464 SEDKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSPKQSENSLGSNKQNFK 523
                            +  + T+ +P+    K       +  +PK +E S G+ K    
Sbjct: 562 ----------------ESPVSDTNKVPEETVAKPVIKAVSKPPTPKDTETS-GTEKPK-- 621

Query: 524 PSLPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGVEENIILGVALDGSKR 583
                       K+ GGT  +  +      ++T  S  S ++  +EENI+LGVAL+GSKR
Sbjct: 622 -----------AKRSGGTIKSTKT------DETDSSTSSASRSTLEENIVLGVALEGSKR 670

Query: 584 TLPIEDEV---PTASGAKDLAACRNGNSANGATTADKNMKRQSPSSPTTSSS 631
           TLPIE+E+   P  + AK+L   R  +  NG   ADK  K  S S P + +S
Sbjct: 682 TLPIEEEIHSPPMETDAKELTGARR-SGGNGPLVADKEQK-DSQSQPNSGAS 670

BLAST of Cla004545 vs. Swiss-Prot
Match: MSL3_ARATH (Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana GN=MSL3 PE=1 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 2.1e-170
Identity = 320/552 (57.97%), Postives = 397/552 (71.92%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           ND N +KS T  I+  YLQPLLLW+GA L+CR LDP+VLP+ + Q +KQR+L F RS+ST
Sbjct: 144 NDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSIST 203

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLAF+ CLSS++QQ QKFF E+   +D RNMGF FA KAVY+A WVAA SLFMELLGFST
Sbjct: 204 VLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFST 263

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATRPFV+NEWIQTKI GYEVSGTVE VG
Sbjct: 264 QKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVEQVG 323

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTIIRG+DREAVHIPNH+F+VN+VRNL+QKTHWRIKTHLAISHLDV+KINNIVADMR
Sbjct: 324 WWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHLDVSKINNIVADMR 383

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVL+KNPQ+EQQ++HRRVFLE++DPENQAL ILISCFVKTS FEEYLCVKEA++LDLL V
Sbjct: 384 KVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEAVLLDLLTV 443

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           IRHH ARLATPIRT+Q+M +++++++  FSD +F    A +NRR ++IEP YK+  +D  
Sbjct: 444 IRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQ--AAMNRRYMLIEPSYKINSDDNS 503

Query: 404 QSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDRKTEVKTGGDADTKMHPKVSMSTSE 463
           +S    S + G+++  P  +S    +   +   S+ K E +  G        K +   + 
Sbjct: 504 KS---PSPSPGQKSPSPGQKS---EERDLQEEPSETKAETENNGSVPVSNAKKENQKAAL 563

Query: 464 DKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSPKQSENSLGSNKQNFKPS 523
             +SN      +    +STSTSD P A+ ++     S+ D   K  ++ +  ++   + +
Sbjct: 564 GSNSN------TGTKGSSTSTSDQPVAQKSEEKKKESVGD-PHKAEKDEVSDDEATIEQT 623

Query: 524 LPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGVEENIILGVALDGSKRTL 583
           L      +   K G   +   S+ R  G   T S        +EEN++LGVALDGSKRTL
Sbjct: 624 L------KSKAKQGSEKNNGESKARDGGGSGTSSL-------LEENLVLGVALDGSKRTL 666

Query: 584 PIEDEVPTASGA 596
           PI DE   ASGA
Sbjct: 684 PI-DEEHKASGA 666

BLAST of Cla004545 vs. Swiss-Prot
Match: YNAI_ECOLI (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) GN=ynaI PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.3e-23
Identity = 77/292 (26.37%), Postives = 146/292 (50.00%), Query Frame = 1

Query: 71  TLICRALDPV---VLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKF-FSEST 130
           T+I  A+  +   V+     + +   V++F+ +    +     L  +I Q +K    +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 131 ESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 190
           + + AR M      + +   + V  + L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIMS-----RIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 191 NFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFT 250
           NF S +M++  RPF + +WI++     E  GTV  +G W  T I   D   +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWIRSPDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFS 230

Query: 251 VNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 310
              V N  + T+ RI T + + + D  K+  IV  +R++L  +P ++Q++     F +  
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFA 290

Query: 311 DPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTM 359
           D    +L I++ CF KT+ + E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 291 D---SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of Cla004545 vs. Swiss-Prot
Match: YNAI_ECO57 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 GN=ynaI PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.3e-23
Identity = 77/292 (26.37%), Postives = 146/292 (50.00%), Query Frame = 1

Query: 71  TLICRALDPV---VLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKF-FSEST 130
           T+I  A+  +   V+     + +   V++F+ +    +     L  +I Q +K    +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 131 ESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 190
           + + AR M      + +   + V  + L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIMS-----RIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 191 NFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFT 250
           NF S +M++  RPF + +WI++     E  GTV  +G W  T I   D   +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWIRSPDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFS 230

Query: 251 VNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 310
              V N  + T+ RI T + + + D  K+  IV  +R++L  +P ++Q++     F +  
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFA 290

Query: 311 DPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTM 359
           D    +L I++ CF KT+ + E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 291 D---SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of Cla004545 vs. Swiss-Prot
Match: Y415_HELPJ (Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 / ATCC 700824) GN=jhp_0969 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.6e-13
Identity = 60/246 (24.39%), Postives = 117/246 (47.56%), Query Frame = 1

Query: 141 KAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF 200
           K VY  +++ AL   ++ LGF+    + + G+G + + LA +++  NF +SV++     F
Sbjct: 367 KVVYFLIFIVALLGVLKQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSF 426

Query: 201 VVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTVNVVRNLS-QKTHW 260
              +WI       EV GTV  +G    T IR  D   + +PN +     +RN S +K   
Sbjct: 427 SQGDWIVCG----EVEGTVVEMG-LRRTTIRAFDNALLSVPNSELAGKPIRNWSRRKVGR 486

Query: 261 RIKTHLAISH-LDVNKINNIVADMRKVLAKNPQV--------EQQRLHRRVFLENV---- 320
           RIK  + +++    + +   V D++++L  +P++        +    +R +F +++    
Sbjct: 487 RIKMEIGLTYSSSQSALQLCVKDIKEMLENHPKIANGADSALQNASDYRYMFKKDIVSID 546

Query: 321 --------------DPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLA 359
                            + ++ IL+ CF KT  +EE+L VKE ++L ++ ++  H    A
Sbjct: 547 DFLGYKNNLFVFLDQFADSSINILVYCFSKTVVWEEWLEVKEDVMLKIMGIVEKHHLSFA 606

BLAST of Cla004545 vs. TrEMBL
Match: A0A0A0L5I6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G114440 PE=4 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 3.8e-304
Identity = 537/589 (91.17%), Postives = 554/589 (94.06%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           ND+NWKKSRTYN+MTL+LQPLLLWTGATLICRALDP+VL TESSQVVKQRVLNFVRSLST
Sbjct: 142 NDSNWKKSRTYNVMTLFLQPLLLWTGATLICRALDPIVLRTESSQVVKQRVLNFVRSLST 201

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLA AYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFST
Sbjct: 202 VLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAAISLFMELLGFST 261

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG
Sbjct: 262 QKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 321

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR
Sbjct: 322 WWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 381

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV
Sbjct: 382 KVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 441

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           IRHH ARLATPIRTMQKMHSDSD+E+VPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK
Sbjct: 442 IRHHGARLATPIRTMQKMHSDSDLENVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 501

Query: 404 QSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDRKTEVKTGGDADTKMHPKVSMSTSE 463
           QSHSRTSRTTGEQNGKP+ARSSGDSKAAKETMSSDRK EVKTGGD DTK H K SMS SE
Sbjct: 502 QSHSRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKIEVKTGGDGDTKKHSKASMSASE 561

Query: 464 DKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSPKQSENSLGSNKQNFKPS 523
           DKSSNELK+KPSS+SAASTS +   +AK TK DADNS+ED  PKQS++SLGSN QNFKPS
Sbjct: 562 DKSSNELKYKPSSKSAASTSDTPTSNAKPTKSDADNSLEDSRPKQSKDSLGSNMQNFKPS 621

Query: 524 LPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGVEENIILGVALDGSKRTL 583
           LP VS PEDVKKPGGTTSAAASQPRIEGEQTTVSNPS  KPGVEENIILGVALDGSKRTL
Sbjct: 622 LPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSTKKPGVEENIILGVALDGSKRTL 681

Query: 584 PIEDEVP-TASGAKDLAACRNGNSANGATTADKNMKRQSPSSPTTSSSE 632
           PIED VP TASGAKDLAA       NGATT DKN KRQSPSSPTTSSSE
Sbjct: 682 PIEDNVPTTASGAKDLAA------FNGATTTDKNTKRQSPSSPTTSSSE 724

BLAST of Cla004545 vs. TrEMBL
Match: A0A0A0L5I6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G114440 PE=4 SV=1)

HSP 1 Score: 33.1 bits (74), Expect = 1.5e+03
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = 1

Query: 23 QFTSQSYNIRLLNVASPSSLL 44
          +FTSQ Y IRLLNV+S SSLL
Sbjct: 26 KFTSQRYGIRLLNVSSSSSLL 46

HSP 2 Score: 729.2 bits (1881), Expect = 4.4e-207
Identity = 388/606 (64.03%), Postives = 472/606 (77.89%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           NDN+WKKS TY+I+  Y+QPLLLW  A LICRALDPV+LPTE+ QVVKQR+LNFVR+LST
Sbjct: 201 NDNSWKKSNTYHIVMSYVQPLLLWMAAILICRALDPVILPTEAGQVVKQRLLNFVRALST 260

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLAFAYCLSS+IQQAQKFF E+ ++SD RNMGFQFA KAVYSAVW+AA SLFMELLGFST
Sbjct: 261 VLAFAYCLSSVIQQAQKFFMETNDTSDTRNMGFQFAGKAVYSAVWIAAFSLFMELLGFST 320

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QKW+TAGGLGTVLLTLAGREIFTNFLSS MIHATRPFVVNEWIQTKIEGYEVSGTVEHVG
Sbjct: 321 QKWVTAGGLGTVLLTLAGREIFTNFLSSAMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 380

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTI+RGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMR
Sbjct: 381 WWSPTIVRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVHKINNIVADMR 440

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVLAKNPQVEQQRLHRRVFLEN++PENQALL+L+SCFVKTSHFEEYLCVKEAI+LDLLRV
Sbjct: 441 KVLAKNPQVEQQRLHRRVFLENINPENQALLVLVSCFVKTSHFEEYLCVKEAILLDLLRV 500

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           I HHRARLATPIRT+QK++SD+D+E+VPF+DSI+  RG   NR +L+IEP YK+ GED+ 
Sbjct: 501 ISHHRARLATPIRTVQKIYSDADLENVPFADSIYS-RGGVSNRPLLLIEPLYKINGEDK- 560

Query: 404 QSHSRTSRTTGEQNGKPVARSSGDSK-------------AAKETMSSDRKTEVKTGGDAD 463
            + +R++R  GE++GK  AR S D+K               KET  SD K + KTGG  +
Sbjct: 561 -TKNRSARPNGERDGKTTARPSSDNKIDAKVGVPPALDSKTKETPPSDNKGDAKTGGTTN 620

Query: 464 TKMHPKVSMSTSEDKSSNELKHKPSSRSAASTSTSDMPD---AKTTKLDADNSMEDCSPK 523
           +     V++ST++ K S+++  K ++++ +  + +D      A+ + LD        S K
Sbjct: 621 SDA-KTVAVSTADPKISDKVVAKSATKTESKVTEADSVSDNGARVSLLDTSTKKSPTS-K 680

Query: 524 QSENSLGSNKQNFKPSLPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGVE 583
           Q +N+   N++N   S    S     K  G +T   A+Q ++E E+T V    ++KP +E
Sbjct: 681 QPKNASLGNQKNTNNSTSSTSEIGAEKHAGFST---AAQVKLETERTAVPKQPMSKPVLE 740

Query: 584 ENIILGVALDGSKRTLPIED---EVPTASGAKDLAACRNGNSANGATTADK-NMKRQSPS 630
           ENI+LGVAL+GSKRTLPIE+     P  +   +LAA R+G    G+ T+DK N + + PS
Sbjct: 741 ENIVLGVALEGSKRTLPIEEGTISPPAHAEVTELAARRSG---QGSPTSDKDNKEGRIPS 795

BLAST of Cla004545 vs. TrEMBL
Match: A0A067KD71_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18699 PE=4 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 1.3e-206
Identity = 394/614 (64.17%), Postives = 463/614 (75.41%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           NDNNWKKSRTY +MT Y+QPLLLWT ATLICR LDPVVLPTE+SQVVKQR+L+FVRSLST
Sbjct: 144 NDNNWKKSRTYYVMTSYIQPLLLWTSATLICRVLDPVVLPTEASQVVKQRLLHFVRSLST 203

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLAFAYCLSS+IQQAQKFF ES E SD RNMGFQFA KAVYSAVWVAA+SLFMELLGFST
Sbjct: 204 VLAFAYCLSSVIQQAQKFFMESNEPSDTRNMGFQFAGKAVYSAVWVAAVSLFMELLGFST 263

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QKWLTAGGLGTVLLTLAGREIFTNFLSS MIHATRPFVVNEWIQTKIEGYEVSGTVEHVG
Sbjct: 264 QKWLTAGGLGTVLLTLAGREIFTNFLSSAMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 323

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTI+RGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLD +KINNIVADMR
Sbjct: 324 WWSPTIVRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDAHKINNIVADMR 383

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVLAKNPQVEQQRLHRRVFL+NV+PENQALLIL+SCFVKTSH EEYLCVKEAI+LDLLRV
Sbjct: 384 KVLAKNPQVEQQRLHRRVFLDNVNPENQALLILVSCFVKTSHHEEYLCVKEAILLDLLRV 443

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           I HHRARLATPIRT+QK++SD+D++++PF+DSI+   G   NR +L+IEP YK+ GED+ 
Sbjct: 444 ISHHRARLATPIRTVQKIYSDADLDNIPFADSIYNCGGVASNRPLLLIEPSYKINGEDKT 503

Query: 404 QSHSRTSRTTGEQNGKPVARSSGDSKA--------AKETMSSDRKTEVKTG------GDA 463
           +S +R  R  G+Q  K ++RS+ +SK         AKE    D K + K G         
Sbjct: 504 KSQTRPGRGAGDQENKGMSRSTSESKGNITKSDAKAKEAPKCDTKADAKNGETPNFYAKG 563

Query: 464 DTKM-HPKVSMSTSEDKSSNELKHKPSSRSAAST----STSDMPDAKTTKLDADNSMEDC 523
           +TK   P VS     DK + +   K S+ + A+T    +   + DA   K  +DN  +  
Sbjct: 564 NTKTGTPSVSDPKVGDKMAVKSSSKTSNSAEATTFESKAAGSVSDATQNKNVSDNKQKSV 623

Query: 524 SPKQSENSLGSNKQNFKPSLPVVSFPEDVKKPGGTTSAAA----SQPRIEGEQTTVSNPS 583
           +P       G+ +QN +   P VS  E      GT  A      SQ +   E+ +V+ PS
Sbjct: 624 NP-------GNIRQNSQFDNPKVSLSE-----AGTDKARGLQEPSQFKQGAERQSVAQPS 683

Query: 584 VTKPGVEENIILGVALDGSKRTLPIEDEVPTASG---AKDLAACRNGNSANGATTADKNM 631
            ++P +EENI+LGVAL+GSKRTLPIED++ + SG    K++A  R   +  G  T +K+ 
Sbjct: 684 -SRPALEENIVLGVALEGSKRTLPIEDDLDSHSGLGEVKEMAGAR--RNGTGTPTGEKDG 742

BLAST of Cla004545 vs. TrEMBL
Match: B9T613_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1054840 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 1.1e-205
Identity = 387/611 (63.34%), Postives = 464/611 (75.94%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           ND+NWKKS TY +MT Y+QPLLLWTGATLICR LDP++LPTE+SQVVKQR+LNFVRSLST
Sbjct: 48  NDSNWKKSGTYYVMTSYVQPLLLWTGATLICRVLDPLILPTEASQVVKQRLLNFVRSLST 107

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLAFAYCLSS+IQQ QKF  ES E SD RNMGFQFA KAVYSAVWVAA+SLFMELLGFST
Sbjct: 108 VLAFAYCLSSVIQQVQKFLIESNEPSDTRNMGFQFAGKAVYSAVWVAAVSLFMELLGFST 167

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QKWLTAGGLGTVLLTLAGREIFTNFLSS MIHATRPFVVNEWIQTKIEGYEVSGTVEHVG
Sbjct: 168 QKWLTAGGLGTVLLTLAGREIFTNFLSSAMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 227

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDV+KI+NIVADMR
Sbjct: 228 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVHKIHNIVADMR 287

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVLAKNPQVEQQRLHRRVFLEN++PENQALLILISCFVKTSH EEYLCVKEAI+LDLLRV
Sbjct: 288 KVLAKNPQVEQQRLHRRVFLENINPENQALLILISCFVKTSHHEEYLCVKEAILLDLLRV 347

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           I HHRARLATPIRT+QK++SD+D+E++PF+DSI+   G+  NR +L+IEP Y++ G+D+ 
Sbjct: 348 ISHHRARLATPIRTVQKIYSDADLENIPFADSIYNRGGSASNRPLLLIEPSYRINGDDKA 407

Query: 404 QSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDR-----KTEVKTG--GDADTKMHPK 463
           +S +R  R  G+Q  K  +RS+ D+KA     S  +     K++ K G   ++D K H K
Sbjct: 408 KSQTRQGRGAGDQENKAASRSTPDTKAGVGPKSDSKTKEAPKSDAKVGETPNSDAKEHIK 467

Query: 464 -VSMSTSEDKSSNELKHKPSSRSAASTST---SDMPDAKTTKLDADNSMEDCSPKQSENS 523
             + S S+ K  +++  K S  S   TS    S  P++K     +DN +++     S+ S
Sbjct: 468 GATTSMSDPKVGDKMSVKSSPNSVPKTSNLAESSSPESKAAASVSDNVIQNKKIPDSKQS 527

Query: 524 L----GSNKQNFKPSLPVVSFPEDVKKPGGTTSAA----ASQPRIEGEQTTVSNPSVTKP 583
                G+ +QN +   P VS  +      GT  A+    + Q +   E+ +    S ++P
Sbjct: 528 KSANPGNGRQNTQLDNPSVSLSD-----AGTEKASGLQESPQSKQGAERKSNGQSSTSRP 587

Query: 584 GVEENIILGVALDGSKRTLPIEDEV---PTASGAKDLAAC-RNGNSANGATTADKNMKRQ 632
            +EENI+LGVAL+GSKRTLPIE+++   PT    K++AA  RNGN++    T  K+   Q
Sbjct: 588 SLEENIVLGVALEGSKRTLPIEEDMTPHPTQGEVKEMAAAGRNGNASPTGETDGKD--GQ 647

BLAST of Cla004545 vs. TrEMBL
Match: A0A061DHM1_THECC (MSCS-like 2 OS=Theobroma cacao GN=TCM_000986 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.4e-205
Identity = 394/607 (64.91%), Postives = 466/607 (76.77%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           +DN+WKKSRT  I T Y QPLLLWTGA LICR LDP+VLP+E+SQVVKQR+LNFVRSLST
Sbjct: 144 SDNSWKKSRTLFITTSYFQPLLLWTGAILICRTLDPLVLPSEASQVVKQRLLNFVRSLST 203

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSD-ARNMGFQFAWKAVYSAVWVAALSLFMELLGFS 163
           VLAFAYCLSSMIQQ QKFF E+ E++D  RNMGFQFA KA+YSAVW+AA+SLFMELLGFS
Sbjct: 204 VLAFAYCLSSMIQQLQKFFMETNETTDDTRNMGFQFAGKAIYSAVWIAAVSLFMELLGFS 263

Query: 164 TQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHV 223
           TQKWLTAGGLGTVLLTLAGREIFTNFLSS MIHATRPFVVNEWIQTKIEGYEVSGTVEHV
Sbjct: 264 TQKWLTAGGLGTVLLTLAGREIFTNFLSSAMIHATRPFVVNEWIQTKIEGYEVSGTVEHV 323

Query: 224 GWWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADM 283
           GWWSPTI+RGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADM
Sbjct: 324 GWWSPTIVRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADM 383

Query: 284 RKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLR 343
           RKVLAKNPQVEQQRLHRRVFLENV+PENQALLIL+SCFVKTSH EEYLCVKEAI+LDLLR
Sbjct: 384 RKVLAKNPQVEQQRLHRRVFLENVNPENQALLILVSCFVKTSHLEEYLCVKEAILLDLLR 443

Query: 344 VIRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDR 403
           VI HHRARLATPIRT+QK++SD+D+E++PF+DS++ H G   NR +L+IEP YK+ GEDR
Sbjct: 444 VISHHRARLATPIRTLQKIYSDADLENIPFADSVYSHGGVPSNRPLLLIEPSYKINGEDR 503

Query: 404 KQSHSRTSRTTGEQNGKPVARSSGDSKA------------AKETMSSDRKTEVKTGG--D 463
            +   R+SR  GEQ+ K  AR   D+KA            AK   S + K + K G   +
Sbjct: 504 IKG--RSSRPAGEQDSKTTARPRADTKADKAGATQKPDSKAKGAPSIEPKADAKIGETPN 563

Query: 464 ADTKMHPKVSM-STSEDKSSNELKHKPSSRSAASTSTSDM----PDAKTTKLDADNSMED 523
           +DTK   KV+  STS+ K+ +++  K  S+S    S++ +    PD K     +DN  ++
Sbjct: 564 SDTKEDLKVAFASTSDLKTDDKVAMKSPSKSVPKKSSNAIETYSPDQKVLDSISDNLPQN 623

Query: 524 CSPKQSENSLGSNKQNFKPSLPVVSFPE-DVKKPGGTTSAAASQPRIEGEQTTVSNPSVT 583
                 +  +   +Q+ K   P  S P+  V K GG      S+   EGE+  V+ P + 
Sbjct: 624 KMVTDKQQKIA--RQSSKLDNPSGSSPDAGVDKAGGLREPLQSKQ--EGEKLPVTQPPIA 683

Query: 584 KPGVEENIILGVALDGSKRTLPIEDEV-PTASGAKDLA-ACRNGNSANGATTADK--NMK 626
           +P +EENI+LGVAL+GSKRTLPIE+ + P+ + AK++A A RNG   +G+T  DK     
Sbjct: 684 RPVLEENIVLGVALEGSKRTLPIEEGMTPSPADAKEIASASRNG---SGSTAEDKKDGQV 741

BLAST of Cla004545 vs. NCBI nr
Match: gi|778676514|ref|XP_011650599.1| (PREDICTED: mechanosensitive ion channel protein 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 1051.6 bits (2718), Expect = 5.5e-304
Identity = 537/589 (91.17%), Postives = 554/589 (94.06%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           ND+NWKKSRTYN+MTL+LQPLLLWTGATLICRALDP+VL TESSQVVKQRVLNFVRSLST
Sbjct: 142 NDSNWKKSRTYNVMTLFLQPLLLWTGATLICRALDPIVLRTESSQVVKQRVLNFVRSLST 201

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLA AYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFST
Sbjct: 202 VLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAAISLFMELLGFST 261

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG
Sbjct: 262 QKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 321

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR
Sbjct: 322 WWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 381

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV
Sbjct: 382 KVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 441

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           IRHH ARLATPIRTMQKMHSDSD+E+VPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK
Sbjct: 442 IRHHGARLATPIRTMQKMHSDSDLENVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 501

Query: 404 QSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDRKTEVKTGGDADTKMHPKVSMSTSE 463
           QSHSRTSRTTGEQNGKP+ARSSGDSKAAKETMSSDRK EVKTGGD DTK H K SMS SE
Sbjct: 502 QSHSRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKIEVKTGGDGDTKKHSKASMSASE 561

Query: 464 DKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSPKQSENSLGSNKQNFKPS 523
           DKSSNELK+KPSS+SAASTS +   +AK TK DADNS+ED  PKQS++SLGSN QNFKPS
Sbjct: 562 DKSSNELKYKPSSKSAASTSDTPTSNAKPTKSDADNSLEDSRPKQSKDSLGSNMQNFKPS 621

Query: 524 LPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGVEENIILGVALDGSKRTL 583
           LP VS PEDVKKPGGTTSAAASQPRIEGEQTTVSNPS  KPGVEENIILGVALDGSKRTL
Sbjct: 622 LPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSTKKPGVEENIILGVALDGSKRTL 681

Query: 584 PIEDEVP-TASGAKDLAACRNGNSANGATTADKNMKRQSPSSPTTSSSE 632
           PIED VP TASGAKDLAA       NGATT DKN KRQSPSSPTTSSSE
Sbjct: 682 PIEDNVPTTASGAKDLAA------FNGATTTDKNTKRQSPSSPTTSSSE 724

BLAST of Cla004545 vs. NCBI nr
Match: gi|778676514|ref|XP_011650599.1| (PREDICTED: mechanosensitive ion channel protein 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 33.1 bits (74), Expect = 2.1e+03
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = 1

Query: 23 QFTSQSYNIRLLNVASPSSLL 44
          +FTSQ Y IRLLNV+S SSLL
Sbjct: 26 KFTSQRYGIRLLNVSSSSSLL 46

HSP 2 Score: 1051.2 bits (2717), Expect = 7.2e-304
Identity = 555/657 (84.47%), Postives = 569/657 (86.61%), Query Frame = 1

Query: 23  QFTSQSYNIRLLNVASPSSLL------------------------------NDNNWKKSR 82
           +FTSQSY IRLLNVAS SSLL                              ND+NWKKSR
Sbjct: 26  KFTSQSYGIRLLNVASSSSLLIQCIQVPVIIDDKPTTGSSWDEGFYSDIDENDSNWKKSR 85

Query: 83  TYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLS 142
           TYN+MTLYLQPLLLWTGATLICRALDPVVL TESSQVVKQRVLNFVRSLSTVLA AYCLS
Sbjct: 86  TYNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLSTVLASAYCLS 145

Query: 143 SMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGL 202
           SMIQQAQKFFSE+TESSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQK LTAGGL
Sbjct: 146 SMIQQAQKFFSENTESSDARNMGFQFAWKAVYSAVWVAAISLFMELLGFSTQKLLTAGGL 205

Query: 203 GTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVE------------ 262
           GTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVE            
Sbjct: 206 GTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEVCMFLLNMYHKL 265

Query: 263 -----HVGWWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKI 322
                HVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDVNKI
Sbjct: 266 LMSKKHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHLAISHLDVNKI 325

Query: 323 NNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEA 382
           NNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVKTSHFEEYLCVKEA
Sbjct: 326 NNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVKTSHFEEYLCVKEA 385

Query: 383 IILDLLRVIRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPY 442
           IILDLLRVIRHH ARLATPIRTMQKMHSDSD+ESVPFSDSIFGHRGATLNRRMLMIEPPY
Sbjct: 386 IILDLLRVIRHHGARLATPIRTMQKMHSDSDLESVPFSDSIFGHRGATLNRRMLMIEPPY 445

Query: 443 KVYGEDRKQSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDRKTEVKTGGDADTKMHP 502
           KV+GEDRKQSHSRTSRTTGEQNGKP+ARSSGDSKAAKETMSSDRKTEVKTGGDADTK H 
Sbjct: 446 KVHGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTGGDADTKKHS 505

Query: 503 KVSMSTSEDKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSPKQSENSLGS 562
           KVSMS SEDKSSNELKHKPSS+SAASTS     DAK TK DADNS+ED SPKQSE+S GS
Sbjct: 506 KVSMSVSEDKSSNELKHKPSSKSAASTSDMPTSDAKPTKSDADNSLEDSSPKQSEDSFGS 565

Query: 563 NKQNFKPSLPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGVEENIILGVA 622
           N QNFKPSLP VS PEDVKKPGGTTSAAASQ RI GEQTTV NPS  KPGVEENIILGVA
Sbjct: 566 NMQNFKPSLPAVSSPEDVKKPGGTTSAAASQQRIVGEQTTVPNPSTKKPGVEENIILGVA 625

Query: 623 LDGSKRTLPIEDEVPT-ASGAKDLAACRNGNSANGATTADKNMKRQSPSSPTTSSSE 632
           LDG KRTLPIED +PT ASGAKDLA        NGAT ADKN K QSPSSPTTSSSE
Sbjct: 626 LDGPKRTLPIEDNLPTAASGAKDLAV------FNGATMADKNTKCQSPSSPTTSSSE 676

BLAST of Cla004545 vs. NCBI nr
Match: gi|659102518|ref|XP_008452175.1| (PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 [Cucumis melo])

HSP 1 Score: 1046.2 bits (2704), Expect = 2.3e-302
Identity = 537/589 (91.17%), Postives = 550/589 (93.38%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           ND+NWKKSRTYN+MTLYLQPLLLWTGATLICRALDPVVL TESSQVVKQRVLNFVRSLST
Sbjct: 142 NDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLST 201

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLA AYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFST
Sbjct: 202 VLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKAVYSAVWVAAISLFMELLGFST 261

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 223
           QK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG
Sbjct: 262 QKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVG 321

Query: 224 WWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 283
           WWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR
Sbjct: 322 WWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMR 381

Query: 284 KVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 343
           KVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV
Sbjct: 382 KVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRV 441

Query: 344 IRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNRRMLMIEPPYKVYGEDRK 403
           IRHH ARLATPIRTMQKMHSDSD+ESVPFSDSIFGHRGATLNRRMLMIEPPYKV+GEDRK
Sbjct: 442 IRHHGARLATPIRTMQKMHSDSDLESVPFSDSIFGHRGATLNRRMLMIEPPYKVHGEDRK 501

Query: 404 QSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDRKTEVKTGGDADTKMHPKVSMSTSE 463
           QSHSRTSRTTGEQNGKP+ARSSGDSKAAKETMSSDRKTEVKTGGDADTK H KVSMS SE
Sbjct: 502 QSHSRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTGGDADTKKHSKVSMSVSE 561

Query: 464 DKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSPKQSENSLGSNKQNFKPS 523
           DKSSNELKHKPSS+SAASTS     DAK TK DADNS+ED SPKQSE+S GSN QNFKPS
Sbjct: 562 DKSSNELKHKPSSKSAASTSDMPTSDAKPTKSDADNSLEDSSPKQSEDSFGSNMQNFKPS 621

Query: 524 LPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGVEENIILGVALDGSKRTL 583
           LP VS PEDVKKPGGTTSAAASQ RI GEQTTV NPS  KPGVEENIILGVALDG KRTL
Sbjct: 622 LPAVSSPEDVKKPGGTTSAAASQQRIVGEQTTVPNPSTKKPGVEENIILGVALDGPKRTL 681

Query: 584 PIEDEVPT-ASGAKDLAACRNGNSANGATTADKNMKRQSPSSPTTSSSE 632
           PIED +PT ASGAKDLA        NGAT ADKN K QSPSSPTTSSSE
Sbjct: 682 PIEDNLPTAASGAKDLAV------FNGATMADKNTKCQSPSSPTTSSSE 724

BLAST of Cla004545 vs. NCBI nr
Match: gi|659102518|ref|XP_008452175.1| (PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 [Cucumis melo])

HSP 1 Score: 36.2 bits (82), Expect = 2.5e+02
Identity = 18/21 (85.71%), Postives = 19/21 (90.48%), Query Frame = 1

Query: 23 QFTSQSYNIRLLNVASPSSLL 44
          +FTSQSY IRLLNVAS SSLL
Sbjct: 26 KFTSQSYGIRLLNVASSSSLL 46

HSP 2 Score: 1035.4 bits (2676), Expect = 4.1e-299
Identity = 537/606 (88.61%), Postives = 550/606 (90.76%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           ND+NWKKSRTYN+MTLYLQPLLLWTGATLICRALDPVVL TESSQVVKQRVLNFVRSLST
Sbjct: 62  NDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLST 121

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLA AYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFST
Sbjct: 122 VLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKAVYSAVWVAAISLFMELLGFST 181

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVE--- 223
           QK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVE   
Sbjct: 182 QKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEVCM 241

Query: 224 --------------HVGWWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLA 283
                         HVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLA
Sbjct: 242 FLLNMYHKLLMSKKHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHLA 301

Query: 284 ISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHF 343
           ISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVKTSHF
Sbjct: 302 ISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVKTSHF 361

Query: 344 EEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNR 403
           EEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSD+ESVPFSDSIFGHRGATLNR
Sbjct: 362 EEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSDLESVPFSDSIFGHRGATLNR 421

Query: 404 RMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDRKTEVKTG 463
           RMLMIEPPYKV+GEDRKQSHSRTSRTTGEQNGKP+ARSSGDSKAAKETMSSDRKTEVKTG
Sbjct: 422 RMLMIEPPYKVHGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTG 481

Query: 464 GDADTKMHPKVSMSTSEDKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSP 523
           GDADTK H KVSMS SEDKSSNELKHKPSS+SAASTS     DAK TK DADNS+ED SP
Sbjct: 482 GDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTSDMPTSDAKPTKSDADNSLEDSSP 541

Query: 524 KQSENSLGSNKQNFKPSLPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGV 583
           KQSE+S GSN QNFKPSLP VS PEDVKKPGGTTSAAASQ RI GEQTTV NPS  KPGV
Sbjct: 542 KQSEDSFGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAASQQRIVGEQTTVPNPSTKKPGV 601

Query: 584 EENIILGVALDGSKRTLPIEDEVPT-ASGAKDLAACRNGNSANGATTADKNMKRQSPSSP 632
           EENIILGVALDG KRTLPIED +PT ASGAKDLA        NGAT ADKN K QSPSSP
Sbjct: 602 EENIILGVALDGPKRTLPIEDNLPTAASGAKDLAV------FNGATMADKNTKCQSPSSP 661

BLAST of Cla004545 vs. NCBI nr
Match: gi|659102506|ref|XP_008452168.1| (PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 1035.4 bits (2676), Expect = 4.1e-299
Identity = 537/606 (88.61%), Postives = 550/606 (90.76%), Query Frame = 1

Query: 44  NDNNWKKSRTYNIMTLYLQPLLLWTGATLICRALDPVVLPTESSQVVKQRVLNFVRSLST 103
           ND+NWKKSRTYN+MTLYLQPLLLWTGATLICRALDPVVL TESSQVVKQRVLNFVRSLST
Sbjct: 142 NDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLST 201

Query: 104 VLAFAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFST 163
           VLA AYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFST
Sbjct: 202 VLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKAVYSAVWVAAISLFMELLGFST 261

Query: 164 QKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVE--- 223
           QK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVE   
Sbjct: 262 QKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEVCM 321

Query: 224 --------------HVGWWSPTIIRGEDREAVHIPNHKFTVNVVRNLSQKTHWRIKTHLA 283
                         HVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLA
Sbjct: 322 FLLNMYHKLLMSKKHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHLA 381

Query: 284 ISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHF 343
           ISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVKTSHF
Sbjct: 382 ISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVKTSHF 441

Query: 344 EEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDMESVPFSDSIFGHRGATLNR 403
           EEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSD+ESVPFSDSIFGHRGATLNR
Sbjct: 442 EEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSDLESVPFSDSIFGHRGATLNR 501

Query: 404 RMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPVARSSGDSKAAKETMSSDRKTEVKTG 463
           RMLMIEPPYKV+GEDRKQSHSRTSRTTGEQNGKP+ARSSGDSKAAKETMSSDRKTEVKTG
Sbjct: 502 RMLMIEPPYKVHGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTG 561

Query: 464 GDADTKMHPKVSMSTSEDKSSNELKHKPSSRSAASTSTSDMPDAKTTKLDADNSMEDCSP 523
           GDADTK H KVSMS SEDKSSNELKHKPSS+SAASTS     DAK TK DADNS+ED SP
Sbjct: 562 GDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTSDMPTSDAKPTKSDADNSLEDSSP 621

Query: 524 KQSENSLGSNKQNFKPSLPVVSFPEDVKKPGGTTSAAASQPRIEGEQTTVSNPSVTKPGV 583
           KQSE+S GSN QNFKPSLP VS PEDVKKPGGTTSAAASQ RI GEQTTV NPS  KPGV
Sbjct: 622 KQSEDSFGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAASQQRIVGEQTTVPNPSTKKPGV 681

Query: 584 EENIILGVALDGSKRTLPIEDEVPT-ASGAKDLAACRNGNSANGATTADKNMKRQSPSSP 632
           EENIILGVALDG KRTLPIED +PT ASGAKDLA        NGAT ADKN K QSPSSP
Sbjct: 682 EENIILGVALDGPKRTLPIEDNLPTAASGAKDLAV------FNGATMADKNTKCQSPSSP 741