Cla004527 (gene) Watermelon (97103) v1

BLAST of Cla004527 vs. Swiss-Prot
Match: SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 2.5e-238
Identity = 419/755 (55.50%), Postives = 539/755 (71.39%), Query Frame = 1

Query: 5   SSAAVDQQTYIIHMDTTKMVTTNPEQWYKAMIDSV-NELSSLDDNEEEASNFAEILYVYK 64
           S+ +  +QTY+IH  TT           K ++ S+ N L + + N+++ S   EI Y+Y+
Sbjct: 35  SNVSSRKQTYVIHTVTTST---------KHIVTSLFNSLQTENINDDDFS-LPEIHYIYE 94

Query: 65  TALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLW 124
            A+SG       F+A L+  +L ++    GF++A P+ELL LHTT+S +FLGL+   GLW
Sbjct: 95  NAMSG-------FSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLW 154

Query: 125 NSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRLIGAS 184
           N ++++SD+IIGL+DTGI PEH+SF+D  + PVPS+W+G C  G  FS S CNK++IGAS
Sbjct: 155 NETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGAS 214

Query: 185 AFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATGMSFS 244
           AF KGYE+IVG++NET  FRS RD+ GHGTHTASTAAG+IV KAN+  Q  G+A+GM F+
Sbjct: 215 AFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFT 274

Query: 245 SRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQN 304
           SRI AYK CW LGCA+ D++AA+D A+ DGVDV+SLSLGG +  FY D IAIA FGA+Q 
Sbjct: 275 SRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQK 334

Query: 305 GVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYYGKNI 364
            +FVSCSAGNSGP++STV N APW+MTVAASYTDRTFP  V++GNR+   GSSLY GK++
Sbjct: 335 NIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKSL 394

Query: 365 NELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAGGAGMI 424
             LPL +N TAG+     FC   SL    V+GKIV+C RG++ RTAKGE+VK +GGA M+
Sbjct: 395 KNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGAAML 454

Query: 425 LINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYGSQAPRV 484
           L++T+ EGEEL ADPHVLPA +LG   GK +L+Y+A + + A AS+ F+GT YG+ AP V
Sbjct: 455 LVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA-ANATASVRFRGTAYGATAPMV 514

Query: 485 AAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCP 544
           AAFSSRGPS  GP++ KPD+ APG+NILA W P  SPS L SD RRV FNIISGTSM+CP
Sbjct: 515 AAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACP 574

Query: 545 HVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASG-GPADPFAFGSGH 604
           H+SG+AAL+KS H DWSPA IKSA+MTTA ++DN+   I D G A     A  FAFG+G+
Sbjct: 575 HISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGN 634

Query: 605 VNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGDLNYPS 664
           V+P +A DPGLVYD +  DY+NYLCSL Y S +I L S   +TC+S   +L PGDLNYPS
Sbjct: 635 VDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPS 694

Query: 665 FSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGEKLSY 724
           F+V +   A   T+  KRTVTNVG P  +Y V +  PKG+ V V+P+ L F    E+LSY
Sbjct: 695 FAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSY 754

Query: 725 QVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
            V++     + +S   SFG LVW+  KY VRSPIA
Sbjct: 755 TVTYDAEASRNSSSS-SFGVLVWICDKYNVRSPIA 770

BLAST of Cla004527 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 2.1e-197
Identity = 364/756 (48.15%), Postives = 484/756 (64.02%), Query Frame = 1

Query: 11  QQTYIIHMDTTKMVT--TNPEQWYKAMIDSVNELSSLDDNEEEASNFAEILYVYKTALSG 70
           ++TY+IHMD + M    TN  QWY + I+SV +  S    +EE  N   ILY Y+TA  G
Sbjct: 34  KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS----QEEEGNNNRILYTYQTAFHG 93

Query: 71  KIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHG--LWNSS 130
                   AA+L+ ++   L +  G +A  P    +LHTT SP FLGL+R     +W   
Sbjct: 94  -------LAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAER 153

Query: 131 NMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRLIGASAFI 190
               D+++G+LDTGIWPE  SF D G+ PVP+ W+G C+ G +F   NCN++++GA  F 
Sbjct: 154 VTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFY 213

Query: 191 KGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATGMSFSSRI 250
           +GYEA  G+++E   ++SPRD DGHGTHTA+T AG+ V  AN      G A GM+  +R+
Sbjct: 214 RGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARV 273

Query: 251 VAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVF 310
            AYKVCW  GC ++DIL+A+D AVADGV VLS+SLGGG  ++ RD+++IA FGA++ GVF
Sbjct: 274 AAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVF 333

Query: 311 VSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYYGKNI--- 370
           VSCSAGN GP   ++ N +PWI TV AS  DR FP TVK+G  + F+G SLY G+ +   
Sbjct: 334 VSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPK 393

Query: 371 -NELPLVY-NNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAGGAG 430
             + PLVY    A     T+FC  G+LD   V GKIV+C+RG   R  KG+ VK AGG G
Sbjct: 394 NKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIG 453

Query: 431 MILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYG-SQA 490
           M+L NT   GEEL AD H+LPA  +G K GK I  Y  +SK +A AS+   GT+ G   +
Sbjct: 454 MVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KATASLEILGTRIGIKPS 513

Query: 491 PRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSM 550
           P VAAFSSRGP+F+  +++KPD+ APGVNILAAW   ++PS L SD RRV FNI+SGTSM
Sbjct: 514 PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSM 573

Query: 551 SCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFAFGS 610
           SCPHVSG+AAL+KS H DWSPAAIKSALMTTAYV DN    ++D   A+  P+ P+  G+
Sbjct: 574 SCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAA--PSSPYDHGA 633

Query: 611 GHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGDLNY 670
           GH++P +A+DPGLVYDI PQ+Y  +LC+   + +Q+ + ++             PG+LNY
Sbjct: 634 GHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNY 693

Query: 671 PSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGEKL 730
           P+ S    +      +TL+RTVTNVG   S Y V ++  KG +V V+P+ L+F S  +KL
Sbjct: 694 PAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKL 753

Query: 731 SYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPI 757
           SY V+F     +       FG LVW S  + VRSP+
Sbjct: 754 SYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPV 772

BLAST of Cla004527 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 2.2e-194
Identity = 363/761 (47.70%), Postives = 489/761 (64.26%), Query Frame = 1

Query: 4   ISSAAVDQQTYIIHMDTTKMVTTNP--EQWYKAMIDSVNELSSLDDNEEEASNFAEILYV 63
           +SS++ DQ TYI+HM  ++M ++      WY + + S+             S+ AE+LY 
Sbjct: 22  VSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYT 81

Query: 64  YKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGL-QRDH 123
           Y+ A+ G       F+ +L+ ++  SL   PG ++  P    +LHTT +P FLGL +   
Sbjct: 82  YENAIHG-------FSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA 141

Query: 124 GLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRLI 183
            L+  +   SD+++G+LDTG+WPE  S+ D+G  P+PS WKG C+AG  F+ S CN++LI
Sbjct: 142 DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLI 201

Query: 184 GASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATGM 243
           GA  F +GYE+ +G ++E+   RSPRD DGHGTHT+STAAG++V  A+      G A GM
Sbjct: 202 GARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGM 261

Query: 244 SFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGA 303
           +  +R+  YKVCW  GC ++DILAA+D A+AD V+VLS+SLGGG   +YRD +AI AF A
Sbjct: 262 APRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAA 321

Query: 304 IQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYYG 363
           ++ G+ VSCSAGN+GPSSS++ N APWI TV A   DR FP    LGN + F G SL+ G
Sbjct: 322 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 381

Query: 364 KNINE--LPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAG 423
           + + +  LP +Y   A +    N C  G+L P KVKGKIV+C+RG N+R  KG+ VK AG
Sbjct: 382 EALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 441

Query: 424 GAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYG- 483
           G GMIL NT   GEEL AD H+LPATT+G KAG  I  Y+ +  +   ASI+  GT  G 
Sbjct: 442 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT-ASISILGTVVGV 501

Query: 484 SQAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISG 543
             +P VAAFSSRGP+ + P+++KPD+ APGVNILAAW     P+ L SD RRV FNIISG
Sbjct: 502 KPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 561

Query: 544 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFA 603
           TSMSCPHVSGLAALLKS H +WSPAAI+SALMTTAY +      + D+  A+G P+ PF 
Sbjct: 562 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFD 621

Query: 604 FGSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGD 663
            G+GHV+P  A++PGL+YD+  +DY+ +LC+L Y S QI  VSR  +TC   ++     D
Sbjct: 622 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS-YSVAD 681

Query: 664 LNYPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKI-NNPKGIAVIVKPEKLSFGSL 723
           LNYPSF+V +            RTVT+VG     Y+VK+ +   G+ + V+P  L+F   
Sbjct: 682 LNYPSFAVNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEA 741

Query: 724 GEKLSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
            EK SY V+F  +   + SG  SFGS+ W  GK+ V SP+A
Sbjct: 742 NEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVA 753

BLAST of Cla004527 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 3.8e-194
Identity = 377/770 (48.96%), Postives = 485/770 (62.99%), Query Frame = 1

Query: 5   SSAAVDQQTYIIHMDTTKMVTTNPEQ--WYKAMIDSVNELSSLDDNEEEASNFAEILYVY 64
           S+++ +  TYI+H+D     +  P    WY + + S+             S+   I++ Y
Sbjct: 19  SASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLT------------SSPPSIIHTY 78

Query: 65  KTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH-- 124
            T   G       F+A+L+++    L   P  ++  P ++  LHTT SP+FLGL+     
Sbjct: 79  DTVFHG-------FSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 138

Query: 125 GLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRLI 184
           GL   S+  SD++IG++DTG+WPE  SF D+GL PVP KWKG C A   F  S CN++L+
Sbjct: 139 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 198

Query: 185 GASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATGM 244
           GA  F  GYEA  G++NET  FRSPRDSDGHGTHTAS +AG  V  A+      GVA GM
Sbjct: 199 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 258

Query: 245 SFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGA 304
           +  +R+ AYKVCW  GC ++DILAA D+AVADGVDV+SLS+GG    +Y D IAI AFGA
Sbjct: 259 APKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGA 318

Query: 305 IQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYYG 364
           I  G+FVS SAGN GP + TV N APW+ TV A   DR FP  VKLGN ++  G S+Y G
Sbjct: 319 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 378

Query: 365 KNINE---LPLVYNNT--AGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVK 424
             ++     PLVY  +   GDG  ++ C  GSLDP+ VKGKIVLC+RG NSR  KGE V+
Sbjct: 379 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 438

Query: 425 LAGGAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIA-SSKSQAK----ASIA 484
             GG GMI+ N  F+GE L AD HVLPAT++GA  G  I  YI+ SSKS++     A+I 
Sbjct: 439 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 498

Query: 485 FQGTKYGSQ-APRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRR 544
           F+GT+ G + AP VA+FS+RGP+   P+++KPDV APG+NILAAWP  + PS + SD RR
Sbjct: 499 FKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 558

Query: 545 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHAS 604
             FNI+SGTSM+CPHVSGLAALLK+AH DWSPAAI+SAL+TTAY  DN    + D   ++
Sbjct: 559 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--EST 618

Query: 605 GGPADPFAFGSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSK 664
           G  +    +GSGHV+P KA DPGLVYDI   DYIN+LC+  Y  T I  ++R +  C   
Sbjct: 619 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 678

Query: 665 RTILQPGDLNYPSFS-VFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKP 724
           R     G+LNYPSFS VF +     ++    RTVTNVG   S Y +KI  P+G  V V+P
Sbjct: 679 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEP 738

Query: 725 EKLSFGSLGEKLSYQVSFVGLGGKEASG--EFSFGSLVWVSGKYAVRSPI 757
           EKLSF  +G+KLS+ V       K + G      G +VW  GK  V SP+
Sbjct: 739 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of Cla004527 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 2.4e-188
Identity = 360/754 (47.75%), Postives = 465/754 (61.67%), Query Frame = 1

Query: 11  QQTYII---HMDTTKMVTTNPEQWYKAMIDSVNELSSLDDNEEEASNFAEILYVYKTALS 70
           ++TYII   H D  +   T+ + WY + ++S + L                LY Y T+  
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHD-WYTSQLNSESSL----------------LYTYTTSFH 86

Query: 71  GKIFKCRCFAAKLSTKKLHSL-SKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWNSS 130
           G       F+A L + +  SL S     L    + L  LHTT +P+FLGL  + G+ +  
Sbjct: 87  G-------FSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLG 146

Query: 131 NMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRLIGASAFI 190
           + ++ +IIG+LDTG+WPE  SF D  +P +PSKWKG C++G  F    CNK+LIGA +F 
Sbjct: 147 SSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFS 206

Query: 191 KGYE-AIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATGMSFSSR 250
           KG++ A  G  +      SPRD DGHGTHT++TAAG+ V  A+F     G A GM+  +R
Sbjct: 207 KGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRAR 266

Query: 251 IVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGV 310
           +  YKVCW  GC  +DILAAMD A+ DGVDVLSLSLGGG+  +YRD IAI AF A++ GV
Sbjct: 267 VATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGV 326

Query: 311 FVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYYGKNINE 370
           FVSCSAGNSGP+ ++V N APW+MTV A   DR FP    LGN +   G SLY G  +  
Sbjct: 327 FVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGT 386

Query: 371 LPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAGGAGMILI 430
            PL      G+   +N C  GSLD S V+GKIV+C+RG N+R  KG  V+ AGG GMI+ 
Sbjct: 387 KPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMA 446

Query: 431 NTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYG-SQAPRVA 490
           NT   GEEL AD H+LPA  +G K G  + +Y+  S S+  A + F+GT      +P VA
Sbjct: 447 NTAASGEELVADSHLLPAIAVGKKTGDLLREYV-KSDSKPTALLVFKGTVLDVKPSPVVA 506

Query: 491 AFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPH 550
           AFSSRGP+ V P+++KPDV  PGVNILA W   + P+ L+ D RR  FNI+SGTSMSCPH
Sbjct: 507 AFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPH 566

Query: 551 VSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFAFGSGHVN 610
           +SGLA LLK+AH +WSP+AIKSALMTTAYV DN  + + D   A    ++P+A GSGHV+
Sbjct: 567 ISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDA--ADNSLSNPYAHGSGHVD 626

Query: 611 PEKASDPGLVYDIAPQDYINYLCSLKYNSTQI-ALVSRGKFTCSSKRTILQPGDLNYPSF 670
           P+KA  PGLVYDI+ ++YI +LCSL Y    I A+V R    CS K     PG LNYPSF
Sbjct: 627 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDPGQLNYPSF 686

Query: 671 SVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGEKLSYQ 730
           SV    K     +   R VTNVG   S Y V +N    + + VKP KLSF S+GEK  Y 
Sbjct: 687 SVLFGGKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 746

Query: 731 VSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
           V+FV   G   + +  FGS+ W + ++ VRSP+A
Sbjct: 747 VTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVA 748

BLAST of Cla004527 vs. TrEMBL
Match: B9IC48_POPTR (Subtilase family protein OS=Populus trichocarpa GN=POPTR_0014s07060g PE=3 SV=2)

HSP 1 Score: 989.9 bits (2558), Expect = 1.9e-285
Identity = 493/758 (65.04%), Postives = 592/758 (78.10%), Query Frame = 1

Query: 5   SSAAVDQQTYIIHMDTTKMVTT-----NPEQWYKAMIDSVNELSSLDDNEEEASNFAEIL 64
           S+A++D+QTYIIHMD  KM        N  QWY+++IDS+ + SS +  EE  + F ++L
Sbjct: 18  SAASIDKQTYIIHMDKNKMPALYDFLGNSRQWYESVIDSITQFSSQEHEEEHETGFPQLL 77

Query: 65  YVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRD 124
           Y Y+T  SG       FAAKLSTK++ +LS++ GFL+A P+ +L LHTTH+P+FLGLQ  
Sbjct: 78  YTYETVTSG-------FAAKLSTKQVEALSRVDGFLSAIPDGMLTLHTTHTPRFLGLQSG 137

Query: 125 HGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRL 184
            GLWN+ N+ASD+I+G+LDTGIWPEH+SFQD G+  VP KWKG C++G KFSPSNCNK+L
Sbjct: 138 KGLWNAQNLASDVIVGILDTGIWPEHVSFQDSGMSAVPLKWKGKCESGTKFSPSNCNKKL 197

Query: 185 IGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATG 244
           IGA AF KGYE+IVGR+NET  +RSPRDS GHGTHTA+TAAGN+V++A+F     G A G
Sbjct: 198 IGARAFFKGYESIVGRINETIDYRSPRDSQGHGTHTAATAAGNLVDEASFYGLANGSAAG 257

Query: 245 MSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFG 304
           M +++RI AYKVCW  GC N D+LAA+D AVADGVDVLSLSLGG A  FY D++AIA+FG
Sbjct: 258 MKYTARIAAYKVCWTSGCTNTDLLAAIDQAVADGVDVLSLSLGGSAKPFYSDSVAIASFG 317

Query: 305 AIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYY 364
           AIQ GVFVSCSAGNSGPS S+V N APWIMTVAASYTDR FPTTVKLGN Q FEG+SLY 
Sbjct: 318 AIQKGVFVSCSAGNSGPSISSVDNNAPWIMTVAASYTDRRFPTTVKLGNGQTFEGASLYT 377

Query: 365 GKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAGG 424
           GK   +LPLVY  TAG G+   +C  GSL    VKGK+V+C+RG N R  KGEQVKLAGG
Sbjct: 378 GKATAQLPLVYAGTAG-GEGAEYCIIGSLKKKLVKGKMVVCKRGMNGRAEKGEQVKLAGG 437

Query: 425 AGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYGSQ 484
            GM+LINT+  GEELFAD H LPAT+LGA AG A+ +Y+ S+K +A ASIAF+GT YG+ 
Sbjct: 438 TGMLLINTETGGEELFADAHFLPATSLGASAGIAVKEYMNSTK-RATASIAFKGTVYGNP 497

Query: 485 APRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTS 544
           AP +AAFSSRGPS VGPDVIKPDVTAPGVNILAAWPP+ SP+ L+SDKR VLFN+ISGTS
Sbjct: 498 APMLAAFSSRGPSSVGPDVIKPDVTAPGVNILAAWPPMTSPTLLKSDKRSVLFNVISGTS 557

Query: 545 MSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFAFG 604
           MSCPHVSGLAALLKS HK WSPAAIKSALMTTAYV+DN+ S I+D G ++   A PFAFG
Sbjct: 558 MSCPHVSGLAALLKSVHKTWSPAAIKSALMTTAYVTDNRGSPIADAGSSNSASATPFAFG 617

Query: 605 SGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGDLN 664
           SGHV+PE ASDPGL+YDI  +DY+NY CSL Y S+QIA VSR   TC   +  LQPGDLN
Sbjct: 618 SGHVDPESASDPGLIYDITIEDYLNYFCSLNYTSSQIAQVSRRNVTCPDNKA-LQPGDLN 677

Query: 665 YPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGEK 724
           YPSF+V  +  A+N  +  KRT+TNVG P S Y VK+  P G++VI++P+ LSF  LG+K
Sbjct: 678 YPSFAVNFEGNARNNRVKYKRTLTNVGTPWSTYAVKVEEPNGVSVILEPKSLSFEKLGQK 737

Query: 725 LSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
           LSY V+FV   GK   G  SFGSLVW+SGKY+VRSPIA
Sbjct: 738 LSYNVTFVSSRGKGREGSSSFGSLVWLSGKYSVRSPIA 765

BLAST of Cla004527 vs. TrEMBL
Match: K7KCA4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_03G019000 PE=3 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 2.6e-282
Identity = 493/762 (64.70%), Postives = 590/762 (77.43%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTTNPEQ-----WYKAMIDSVNELSSLDDNEEEASNF 60
           M+  S A +++QTYIIHMD TK+  T   Q     W+K++ID ++E SS  + EE     
Sbjct: 13  MVTNSVAVMNKQTYIIHMDKTKIKATVHSQDKTKPWFKSVIDFISEASSSSEEEEAP--- 72

Query: 61  AEILYVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120
            ++LYVY+T++ G       FAA+LS K+L  L++I GFL+A P+ELL LHTT+SP FLG
Sbjct: 73  -QLLYVYETSMFG-------FAAQLSNKQLEYLNQIDGFLSAIPDELLTLHTTYSPHFLG 132

Query: 121 LQRDHGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNC 180
           LQ   GLW++SN+ASD+IIG+LDTGIWPEHISFQD GL  VPS+WKG C+ G  FS S C
Sbjct: 133 LQNGKGLWSASNLASDVIIGVLDTGIWPEHISFQDTGLSKVPSRWKGACEVGTNFSSSCC 192

Query: 181 NKRLIGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMG 240
           NK+L+GA  F++GYE   GR+NET  +RS RD+ GHGTHTASTAAGN+V+ A+F     G
Sbjct: 193 NKKLVGARVFLQGYEKSAGRINETLDYRSARDAQGHGTHTASTAAGNMVSNASFFGLAGG 252

Query: 241 VATGMSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
            A+GM ++SRI AYKVCW LGCAN+DILAA+D AVADGVDVLSLSLGG A  +Y D+IAI
Sbjct: 253 SASGMRYTSRIAAYKVCWRLGCANSDILAAIDQAVADGVDVLSLSLGGIAKPYYNDSIAI 312

Query: 301 AAFGAIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGS 360
           A+FGA Q GVFVSCSAGNSGPSSST GN APWIMTVAASYTDR+FPT VKLGN +VF+GS
Sbjct: 313 ASFGATQKGVFVSCSAGNSGPSSSTAGNVAPWIMTVAASYTDRSFPTQVKLGNGKVFKGS 372

Query: 361 SLYYGKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVK 420
           SLY GK  ++LPLVY N++   +   +CT GSLDP  VKGKIV CERG NSRT KGE+VK
Sbjct: 373 SLYKGKKTSQLPLVYRNSSRAQRTAQYCTKGSLDPKLVKGKIVACERGINSRTGKGEEVK 432

Query: 421 LAGGAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTK 480
           +AGGAGMIL+N++ +GEELFADPHVLPAT+LG+ A K I  YI  S     ASI+F GT 
Sbjct: 433 MAGGAGMILLNSENQGEELFADPHVLPATSLGSSASKTIRSYIFHSAKAPTASISFLGTT 492

Query: 481 YGSQAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           YG  AP +AAFSSRGPS VGPDVIKPDVTAPGVNILAAWPP  SPS L+SDKR VLFNI+
Sbjct: 493 YGDTAPVMAAFSSRGPSSVGPDVIKPDVTAPGVNILAAWPPTTSPSMLKSDKRSVLFNIV 552

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADP 600
           SGTSMSCPHVSG+AAL+KS HKDWSPAAIKSALMTTA  S+NK + ISD G  +   ADP
Sbjct: 553 SGTSMSCPHVSGIAALIKSVHKDWSPAAIKSALMTTASTSNNKGAPISDNGSNNSAFADP 612

Query: 601 FAFGSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQP 660
           FAFGSGHVNPE+ASDPGLVYDI  +DY+NYLCSLKY S+QIA++S+G F C +K++ L  
Sbjct: 613 FAFGSGHVNPERASDPGLVYDITTKDYLNYLCSLKYTSSQIAILSKGNFKC-AKKSALHA 672

Query: 661 GDLNYPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGS 720
           G LNYPSF+V     A+N ++T KR VTNVG P S Y VK+  PKG++V V+P  + F  
Sbjct: 673 GGLNYPSFAVLFDTSARNASVTYKRVVTNVGNPSSSYAVKVEEPKGVSVTVEPRNIGFRK 732

Query: 721 LGEKLSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
           +G+KLSY+VSFV  G    +G  SFGSL WVSGKYAVRSPIA
Sbjct: 733 IGDKLSYKVSFVSYGRTAVAGSSSFGSLTWVSGKYAVRSPIA 762

BLAST of Cla004527 vs. TrEMBL
Match: A0A0L9VRL6_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan11g043200 PE=3 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 6.3e-281
Identity = 491/768 (63.93%), Postives = 592/768 (77.08%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTTNPEQ-----------WYKAMIDSVNELSSLDDNE 60
           M+  S A V++QTYI+HMD TKM  +   Q           W+K++ID ++E S     E
Sbjct: 13  MVTKSVAVVEKQTYIVHMDKTKMEASVHSQGLAKPWFKSKPWFKSVIDFISEASF---EE 72

Query: 61  EEASNFAEILYVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTH 120
           EE     ++LYVY+T+L G       FAA+LS K+L  L+++ GF+AA P+ELL LHTT+
Sbjct: 73  EEGGGEPQLLYVYETSLFG-------FAAQLSNKQLEYLNQVDGFVAALPDELLTLHTTY 132

Query: 121 SPQFLGLQRDHGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPK 180
           +P FLGLQ   GLW++SN+ASD+IIG+LDTGIWPEHISFQD GL  VPS+WKG C+AG  
Sbjct: 133 TPHFLGLQEGEGLWSASNLASDVIIGVLDTGIWPEHISFQDTGLSKVPSRWKGACEAGTN 192

Query: 181 FSPSNCNKRLIGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANF 240
           FS S+CNK+L+GA  F++GYE   GR+NET  +RS RD+ GHG+HTASTAAGN+V  A+F
Sbjct: 193 FSASSCNKKLVGARVFLQGYEKFAGRINETMDYRSARDAQGHGSHTASTAAGNMVKNASF 252

Query: 241 NNQGMGVATGMSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFY 300
                G A+GM ++SRI AYKVCW LGCAN+DILAA+D AVADGVDVLSLSLGG A  +Y
Sbjct: 253 FGLASGSASGMRYTSRIAAYKVCWRLGCANSDILAAIDKAVADGVDVLSLSLGGSARPYY 312

Query: 301 RDNIAIAAFGAIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNR 360
            D+IAIA+FGA QNGVFVSCSAGNSGP SSTVGN APWIMTVAASYTDR+FPT VKLGN 
Sbjct: 313 SDSIAIASFGATQNGVFVSCSAGNSGPFSSTVGNVAPWIMTVAASYTDRSFPTQVKLGNG 372

Query: 361 QVFEGSSLYYGKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTA 420
           ++F+GSSLY GK  N+LPLVY N++ + +   +CT GSLDP  VKGKIV CERG NSRTA
Sbjct: 373 KLFKGSSLYKGKKTNQLPLVYVNSSKEHRTAQYCTKGSLDPKFVKGKIVACERGINSRTA 432

Query: 421 KGEQVKLAGGAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASI 480
           KGE+VK+AGGAGMIL+N++ +GEELFAD HVLP T+LGA A K I  YI S+K+   ASI
Sbjct: 433 KGEEVKMAGGAGMILLNSENQGEELFADHHVLPGTSLGASASKIIRSYIHSAKAPT-ASI 492

Query: 481 AFQGTKYGSQAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRR 540
           +FQGT YG  AP +AAFSSRGPS VG DVIKPDVTAPGVNILAAWPPI SPS L+SDK  
Sbjct: 493 SFQGTAYGDPAPAMAAFSSRGPSAVGGDVIKPDVTAPGVNILAAWPPITSPSMLKSDKTS 552

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHAS 600
            +FNI+SGTSMSCPHVSG+AAL+KSAH  WSPAAIKSALMTTA VS+NK + I+D G  +
Sbjct: 553 AVFNIVSGTSMSCPHVSGIAALIKSAHNGWSPAAIKSALMTTASVSNNKGAAIADYGSKT 612

Query: 601 GGPADPFAFGSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSK 660
              ADPFAFGSGHVNPE+ASDPGLVYDI  +DY+NYLCSLKY  +QIAL+S+G FTC  K
Sbjct: 613 SALADPFAFGSGHVNPERASDPGLVYDITTKDYLNYLCSLKYTPSQIALLSKGNFTC-PK 672

Query: 661 RTILQPGDLNYPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPE 720
           ++ LQ GDLNYPSF+V    +A   ++T KR VTNVG P+  Y VK+  P G++V V+P 
Sbjct: 673 KSALQAGDLNYPSFAVLFGTRALKASVTFKRVVTNVGNPKISYAVKVEEPNGVSVRVEPR 732

Query: 721 KLSFGSLGEKLSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
            +SF   G+K+SY+VSFV  G    +G  SFGSL WVSGKYAVRSPIA
Sbjct: 733 NISFRKTGQKMSYKVSFVSNGNTTVTGNSSFGSLTWVSGKYAVRSPIA 768

BLAST of Cla004527 vs. TrEMBL
Match: A0A0S3SYR0_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.09G141200 PE=3 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 6.3e-281
Identity = 491/768 (63.93%), Postives = 592/768 (77.08%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTTNPEQ-----------WYKAMIDSVNELSSLDDNE 60
           M+  S A V++QTYI+HMD TKM  +   Q           W+K++ID ++E S     E
Sbjct: 13  MVTKSVAVVEKQTYIVHMDKTKMEASVHSQGLAKPWFKSKPWFKSVIDFISEASF---EE 72

Query: 61  EEASNFAEILYVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTH 120
           EE     ++LYVY+T+L G       FAA+LS K+L  L+++ GF+AA P+ELL LHTT+
Sbjct: 73  EEGGGEPQLLYVYETSLFG-------FAAQLSNKQLEYLNQVDGFVAALPDELLTLHTTY 132

Query: 121 SPQFLGLQRDHGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPK 180
           +P FLGLQ   GLW++SN+ASD+IIG+LDTGIWPEHISFQD GL  VPS+WKG C+AG  
Sbjct: 133 TPHFLGLQEGEGLWSASNLASDVIIGVLDTGIWPEHISFQDTGLSKVPSRWKGACEAGTN 192

Query: 181 FSPSNCNKRLIGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANF 240
           FS S+CNK+L+GA  F++GYE   GR+NET  +RS RD+ GHG+HTASTAAGN+V  A+F
Sbjct: 193 FSASSCNKKLVGARVFLQGYEKFAGRINETMDYRSARDAQGHGSHTASTAAGNMVKNASF 252

Query: 241 NNQGMGVATGMSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFY 300
                G A+GM ++SRI AYKVCW LGCAN+DILAA+D AVADGVDVLSLSLGG A  +Y
Sbjct: 253 FGLASGSASGMRYTSRIAAYKVCWRLGCANSDILAAIDKAVADGVDVLSLSLGGSARPYY 312

Query: 301 RDNIAIAAFGAIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNR 360
            D+IAIA+FGA QNGVFVSCSAGNSGP SSTVGN APWIMTVAASYTDR+FPT VKLGN 
Sbjct: 313 SDSIAIASFGATQNGVFVSCSAGNSGPFSSTVGNVAPWIMTVAASYTDRSFPTQVKLGNG 372

Query: 361 QVFEGSSLYYGKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTA 420
           ++F+GSSLY GK  N+LPLVY N++ + +   +CT GSLDP  VKGKIV CERG NSRTA
Sbjct: 373 KLFKGSSLYKGKKTNQLPLVYVNSSKEHRTAQYCTKGSLDPKFVKGKIVACERGINSRTA 432

Query: 421 KGEQVKLAGGAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASI 480
           KGE+VK+AGGAGMIL+N++ +GEELFAD HVLP T+LGA A K I  YI S+K+   ASI
Sbjct: 433 KGEEVKMAGGAGMILLNSENQGEELFADHHVLPGTSLGASASKIIRSYIHSAKAPT-ASI 492

Query: 481 AFQGTKYGSQAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRR 540
           +FQGT YG  AP +AAFSSRGPS VG DVIKPDVTAPGVNILAAWPPI SPS L+SDK  
Sbjct: 493 SFQGTAYGDPAPAMAAFSSRGPSAVGGDVIKPDVTAPGVNILAAWPPITSPSMLKSDKTS 552

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHAS 600
            +FNI+SGTSMSCPHVSG+AAL+KSAH  WSPAAIKSALMTTA VS+NK + I+D G  +
Sbjct: 553 AVFNIVSGTSMSCPHVSGIAALIKSAHNGWSPAAIKSALMTTASVSNNKGAAIADYGSKT 612

Query: 601 GGPADPFAFGSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSK 660
              ADPFAFGSGHVNPE+ASDPGLVYDI  +DY+NYLCSLKY  +QIAL+S+G FTC  K
Sbjct: 613 SALADPFAFGSGHVNPERASDPGLVYDITTKDYLNYLCSLKYTPSQIALLSKGNFTC-PK 672

Query: 661 RTILQPGDLNYPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPE 720
           ++ LQ GDLNYPSF+V    +A   ++T KR VTNVG P+  Y VK+  P G++V V+P 
Sbjct: 673 KSALQAGDLNYPSFAVLFGTRALKASVTFKRVVTNVGNPKISYAVKVEEPNGVSVRVEPR 732

Query: 721 KLSFGSLGEKLSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
            +SF   G+K+SY+VSFV  G    +G  SFGSL WVSGKYAVRSPIA
Sbjct: 733 NISFRKTGQKMSYKVSFVSNGNTTVTGNSSFGSLTWVSGKYAVRSPIA 768

BLAST of Cla004527 vs. TrEMBL
Match: V7AQQ3_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G088700g PE=3 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 9.1e-280
Identity = 490/762 (64.30%), Postives = 588/762 (77.17%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTT-----NPEQWYKAMIDSVNELSSLDDNEEEASNF 60
           M+  S A +++QTYI+HMD TK+  +     N + W+K++ID ++E S      EE    
Sbjct: 13  MVVKSVAVMEKQTYIVHMDKTKIEASVHSQDNIKPWFKSVIDFISEASL-----EEEERA 72

Query: 61  AEILYVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120
            ++LYVY+T+L G       FAA+LS K+L  L+++ GF+AA P+ELL LHTT+SP FLG
Sbjct: 73  PQLLYVYETSLFG-------FAAQLSNKQLEYLNQVDGFVAAIPDELLTLHTTYSPHFLG 132

Query: 121 LQRDHGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNC 180
           LQ   GLW++SN+ASD+IIG+LDTGIWPEH+SFQD GL  VPS+WKG C+AG  FS S+C
Sbjct: 133 LQEGKGLWSASNLASDVIIGVLDTGIWPEHVSFQDTGLSKVPSRWKGACEAGTNFSASSC 192

Query: 181 NKRLIGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMG 240
           NK+L+GA  F++GYE   GR+NET  +RS RD+ GHG+HTASTAAGN VN A+F     G
Sbjct: 193 NKKLVGARVFLQGYEKFAGRINETLDYRSARDAQGHGSHTASTAAGNTVNNASFFGLASG 252

Query: 241 VATGMSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
            ATGM ++SRI AYKVCW LGCAN+DILAA+D AVADGVDVLSLSLGG A  +Y D+IAI
Sbjct: 253 SATGMRYTSRIAAYKVCWRLGCANSDILAAIDKAVADGVDVLSLSLGGIAKPYYNDSIAI 312

Query: 301 AAFGAIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGS 360
           A+FGA Q GVFVSCSAGNSGPSSSTVGN APWIMTVAASYTDR+FPT VKLGN + F+GS
Sbjct: 313 ASFGATQKGVFVSCSAGNSGPSSSTVGNVAPWIMTVAASYTDRSFPTQVKLGNGKFFKGS 372

Query: 361 SLYYGKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVK 420
           SLY GK  N+LPLVY N++   +   +CT GSLDP  VKGKIV CERG NSRT KGE+VK
Sbjct: 373 SLYKGKQTNQLPLVYGNSSKAQRTAQYCTKGSLDPKFVKGKIVACERGINSRTGKGEEVK 432

Query: 421 LAGGAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTK 480
           +AGGAGMIL+N++ +GEELFADPHVLP T+LGA A K I  YI S K+   ASI+F GT 
Sbjct: 433 MAGGAGMILLNSENQGEELFADPHVLPGTSLGASASKIIRSYIHSEKAPT-ASISFLGTA 492

Query: 481 YGSQAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           YG  AP +AAFSSRGPS VG DVIKPDVTAPGVNILAAWPP  SPS L+SDKR  L+NI+
Sbjct: 493 YGDPAPVMAAFSSRGPSAVGGDVIKPDVTAPGVNILAAWPPTTSPSMLKSDKRSALYNIV 552

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADP 600
           SGTSMSCPHVSG+AAL+KS HK WSPAAIKSALMTTA +S+NK S I+D G  +   ADP
Sbjct: 553 SGTSMSCPHVSGIAALIKSVHKGWSPAAIKSALMTTASISNNKGSAIADYGSKNSAFADP 612

Query: 601 FAFGSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQP 660
           FAFGSGHVNPE+ASDPGLVYDI  +DY+NYLCSLKY S+QIAL+S+G F C +K++ L+ 
Sbjct: 613 FAFGSGHVNPERASDPGLVYDITTKDYLNYLCSLKYTSSQIALLSKGNFKC-AKKSALRA 672

Query: 661 GDLNYPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGS 720
           GDLNYPSF+V     A N ++  KR VTNVG P+  Y VK+  PKG++V V+P  +SF  
Sbjct: 673 GDLNYPSFAVLFGTSALNASVRYKRIVTNVGNPKISYAVKVEEPKGVSVRVEPTNISFRK 732

Query: 721 LGEKLSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
            GEKLSY+VSFV       SG  SFGS+ WVSGKYAVRSPIA
Sbjct: 733 TGEKLSYKVSFVSNENTTVSGSSSFGSITWVSGKYAVRSPIA 760

BLAST of Cla004527 vs. NCBI nr
Match: gi|659109540|ref|XP_008454762.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1335.1 bits (3454), Expect = 0.0e+00
Identity = 670/756 (88.62%), Postives = 705/756 (93.25%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTTNPEQWYKAMIDSVNELSSLDDNEEEASNFAEILY 60
           MLAISSA VDQQTYIIHMDTTKMVT NPEQWY  +IDSVNELSSLDDNEE ASN AEILY
Sbjct: 12  MLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDNEE-ASNAAEILY 71

Query: 61  VYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH 120
           VYKTALSG       FAAKL++KKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH
Sbjct: 72  VYKTALSG-------FAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH 131

Query: 121 GLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRLI 180
           GLWN SN+ASDIIIGLLDTGIWPEHISFQDKGL  VP KWKGICQ GP+FS SNCNK+LI
Sbjct: 132 GLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCNKKLI 191

Query: 181 GASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATGM 240
           GASA+IKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG+IV+ A+F NQGMGVA+GM
Sbjct: 192 GASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGVASGM 251

Query: 241 SFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGA 300
            F+SRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGG+ SFY+DNIAIAAFGA
Sbjct: 252 RFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGA 311

Query: 301 IQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYYG 360
           IQ GVFVSCSAGNSGPS STVGNAAPWIMTVAASYTDRTFPTTVKLGN QVFEGSSLYYG
Sbjct: 312 IQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYG 371

Query: 361 KNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAGGA 420
           K+INELPLVYNNTAGDGQETN C AGSLDPS VKGKIV+CERG+ SRT KGEQVKLAGG 
Sbjct: 372 KSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKLAGGT 431

Query: 421 GMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYGSQA 480
           GMILINTQFEGEELFADPHVLPATTLGA AGKAILDYIASSK+QAKASI F+GTKYGSQA
Sbjct: 432 GMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKYGSQA 491

Query: 481 PRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSM 540
           PRVAAFSSRGPS VGPDVIKPDVTAPGVNILAAWPPIVSPSEL SD RRV+FNIISGTSM
Sbjct: 492 PRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNIISGTSM 551

Query: 541 SCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFAFGS 600
           SCPHVSGLAALLKSAH DWSPAAIKSALMTTAYV+D+KMSLISDVG A+G PA PF FGS
Sbjct: 552 SCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPFTFGS 611

Query: 601 GHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGDLNY 660
           GHV+PEKASDPGL+YDI PQDYINYLCSLKYNS+QIALVSRG  TCSSKRT+++PGDLNY
Sbjct: 612 GHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPGDLNY 671

Query: 661 PSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGEKL 720
           PSFSVFMKKKAK V+ITLKRTVTNVGI RSDYTVKINNPKG+ VIVKPEKLSFGSLGE+L
Sbjct: 672 PSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSLGEQL 731

Query: 721 SYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPI 757
           SY+VSFV LGGKEA  +FSFGSLVW+SGKYAVRSPI
Sbjct: 732 SYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPI 759

BLAST of Cla004527 vs. NCBI nr
Match: gi|449445359|ref|XP_004140440.1| (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis sativus])

HSP 1 Score: 1330.9 bits (3443), Expect = 0.0e+00
Identity = 665/757 (87.85%), Postives = 702/757 (92.73%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTTNPEQWYKAMIDSVNELSSLDDNEEEASNFAEILY 60
           MLA+SSA VDQQTYIIHMD TKMVT  PEQWY  +IDSVN+LSSLDDNEEEASN AEILY
Sbjct: 13  MLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDNEEEASNAAEILY 72

Query: 61  VYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH 120
           VYKTALSG       FAAKL++KKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH
Sbjct: 73  VYKTALSG-------FAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDH 132

Query: 121 GLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRLI 180
           GLWNSSN+ASDIIIGLLDTG+WPEHISFQD+ L  VP KWKGICQ GP+FS SNCNK+LI
Sbjct: 133 GLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNCNKKLI 192

Query: 181 GASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATGM 240
           GAS +IKGYEAIVGRLNETG FRSPRDS+GHGTHTASTAAG+IVN A+F NQGMGVA+G+
Sbjct: 193 GASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMGVASGI 252

Query: 241 SFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGA 300
            F+SRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGG+ SFY+DNIAIAAFGA
Sbjct: 253 RFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGA 312

Query: 301 IQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYYG 360
           I+ GVFVSCSAGNSGPS STVGNAAPWIMTVAASYTDRTFPTTVKLGN QVFEGSSLYYG
Sbjct: 313 IEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYG 372

Query: 361 KNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAGGA 420
           K+INELPLVYNNTAGDGQETNFC AGSLDPS VKGKIV+CERG  SRT KGEQVKLAGGA
Sbjct: 373 KSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVKLAGGA 432

Query: 421 GMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYGSQA 480
           GMILINT+FEGEELFADPH+LPATTLGA AGKAILDY ASSK+QAKA I F+GTKYGSQA
Sbjct: 433 GMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTKYGSQA 492

Query: 481 PRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSM 540
           PRVAAFSSRGPS VGPDVIKPDVTAPGVNILAAWPPIVSPSELESD RRVLFNIISGTSM
Sbjct: 493 PRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNIISGTSM 552

Query: 541 SCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFAFGS 600
           SCPHVSGLAALLKSAH DWSPAAIKSALMTTAY++DNKMSLISDVG A+G PA PF FGS
Sbjct: 553 SCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATPFTFGS 612

Query: 601 GHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGDLNY 660
           GHV+PEKASDPGL+YDI PQDYINYLCSLKYNSTQIALVSRG FTCSSKRT+++PGDLNY
Sbjct: 613 GHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKPGDLNY 672

Query: 661 PSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGEKL 720
           PSFSVFMKKKAK V+ITLKRTVTNVGI RSDYTVKINNPKGI VIVKPEKLSFGSLGE+L
Sbjct: 673 PSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGSLGEQL 732

Query: 721 SYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
           SYQV FV LGGKEA   FSFGSLVW+SGKYAVRSPIA
Sbjct: 733 SYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIA 762

BLAST of Cla004527 vs. NCBI nr
Match: gi|659109542|ref|XP_008454764.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 621/759 (81.82%), Postives = 669/759 (88.14%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTTNPEQWYKAMIDSVNELSSLD--DNEEEASNFAEI 60
           ML  S  A+DQQ+YI+HMDTTKM TTNPEQWY A+I SVN+LSSLD  +NEE+A N AEI
Sbjct: 14  MLTTSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLDANNNEEQALNIAEI 73

Query: 61  LYVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQR 120
           LYVYKT +SG       F AKLS+K LH LSK+PGF+AA+PNELLQLHTTHSP+FLGLQR
Sbjct: 74  LYVYKTVISG-------FCAKLSSKNLHFLSKVPGFIAASPNELLQLHTTHSPKFLGLQR 133

Query: 121 DHGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKR 180
            HGLWNSSN+ASDIIIG+LDTGIWPEHISFQDK LPPVPSKWKGICQ GP FS SNCNK+
Sbjct: 134 GHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPSFSLSNCNKK 193

Query: 181 LIGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVAT 240
           LIGA  FI+ YEA VGRLN TGTFRS RDSDGHGTHTASTAAGN VN+A+F NQGMGVAT
Sbjct: 194 LIGARTFIQAYEAAVGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASFYNQGMGVAT 253

Query: 241 GMSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAF 300
           GM F+SRI AYKVCWP GCA+ADILAAMD AVADGVDVLS+SLGGG+   Y D IAIAAF
Sbjct: 254 GMRFTSRIAAYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAF 313

Query: 301 GAIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLY 360
           GAIQ GVFVSCSAGNSGP  STVGN APW+MTVAASYTDRTFPTTV+LGN  VFEGSSLY
Sbjct: 314 GAIQKGVFVSCSAGNSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMVFEGSSLY 373

Query: 361 YGKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAG 420
           +GKN+ E PLVYNNTAGDG+ETNFCT GSLDP+ VKGKI +CERG+NSRT KGEQVKLAG
Sbjct: 374 FGKNLKETPLVYNNTAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKGEQVKLAG 433

Query: 421 GAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYGS 480
           GAGMILINT  EGE+L AD HVLPAT++G  AGK+IL+YIASSK QAKASI F+GTKYGS
Sbjct: 434 GAGMILINTILEGEDLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASIMFKGTKYGS 493

Query: 481 QAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGT 540
           +APRVAAFSSRGPSF  P VIKPD+TAPGVNILAAWPPIVSPSELESDKRRVLFNIISGT
Sbjct: 494 RAPRVAAFSSRGPSFYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGT 553

Query: 541 SMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFAF 600
           SMSCPHVSGLAALLKS HKDWSPAAIKSALMTTAYV+DNK  LISDVG ASGGPADPFAF
Sbjct: 554 SMSCPHVSGLAALLKSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRASGGPADPFAF 613

Query: 601 GSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGDL 660
           GSGHV+PEKASDPGLVYDIAPQDYI YLCSLKYNSTQIALVSRGKFTCSSKRT  QPGDL
Sbjct: 614 GSGHVDPEKASDPGLVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSKRTFFQPGDL 673

Query: 661 NYPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGE 720
           NYPSFSVFM KK KNV  T KRTVTNVGIPRSDYTV+I NPKGI +IVKPEKLSF  LGE
Sbjct: 674 NYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLSFVKLGE 733

Query: 721 KLSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
           KLSY+VSFV LG +++  +FSFGSLVW SG Y VRSPIA
Sbjct: 734 KLSYKVSFVALGKRKSLDDFSFGSLVWHSGTYVVRSPIA 764

BLAST of Cla004527 vs. NCBI nr
Match: gi|778730654|ref|XP_004140477.2| (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis sativus])

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 615/760 (80.92%), Postives = 671/760 (88.29%), Query Frame = 1

Query: 1   MLAISSAAVDQQTYIIHMDTTKMVTTNPEQWYKAMIDSVNELSSL---DDNEEEASNFAE 60
           ML  SS A+DQQTYI+HMDTTKM T NPEQWY A+IDSVN+LSSL   ++++EEA N AE
Sbjct: 14  MLTTSSVAMDQQTYIVHMDTTKMDTPNPEQWYTAIIDSVNQLSSLYGDNNDDEEALNAAE 73

Query: 61  ILYVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQ 120
           ILYVYKT +SG       F+AKLS++ LHSLSK+PGF+AATPNELLQLHTTHSPQFLGLQ
Sbjct: 74  ILYVYKTVISG-------FSAKLSSRNLHSLSKVPGFVAATPNELLQLHTTHSPQFLGLQ 133

Query: 121 RDHGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNK 180
           R HGLWNSSN+ASDIIIG+LDTGIWPEHISFQDKGLPPVPSKWKGICQ GP FS SNCNK
Sbjct: 134 RGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGPNFSHSNCNK 193

Query: 181 RLIGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVA 240
           +LIGA  FI+ YEA VGRLN TG FRS RDS+GHGTHTASTAAGN +N+A+F NQGMGVA
Sbjct: 194 KLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRASFYNQGMGVA 253

Query: 241 TGMSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAA 300
           TGM F+SRI +YKVCWP GCA+ADILAAMD AVADGVDVLS+SLGGG+   Y D IAIAA
Sbjct: 254 TGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAA 313

Query: 301 FGAIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSL 360
           FGAIQ GVFVSCSAGNSGP  STV N APW+MTVAASYTDRTFPTTV+LGN +VFEGSS 
Sbjct: 314 FGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGNGKVFEGSSS 373

Query: 361 YYGKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLA 420
           Y+GKN+ E+PLVYNNTAGDGQETNFCTAGSLDP+ V+GKIV+CERG+NSRT KGEQVKLA
Sbjct: 374 YFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRTKKGEQVKLA 433

Query: 421 GGAGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYG 480
           GGAGMILINT  EGE+L AD HVLPAT++GA A K+IL+YIASSK QAKASI F+GTKYG
Sbjct: 434 GGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKASIIFKGTKYG 493

Query: 481 SQAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISG 540
           S+APRVAAFSSRGPSF  P VIKPD+TAPGVNILAAWPPIVSPSELESDKRRVLFNIISG
Sbjct: 494 SRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISG 553

Query: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFA 600
           TSMSCPHVSGLAAL+KS HKDWSPAAIKSALMTTAYV+DNK  LISDVG ASGGPAD FA
Sbjct: 554 TSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRASGGPADSFA 613

Query: 601 FGSGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGD 660
           FGSGHV+PEKAS PGL+YDIAPQDYI YLCSLKY STQI+LVSRGKFTCSSK T  QPGD
Sbjct: 614 FGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSSKNTFSQPGD 673

Query: 661 LNYPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLG 720
           LNYPSFSVFM KK KNV  T KRTVTNVGIPRSDYTV+INNPKGI +IVKPEKL+F  LG
Sbjct: 674 LNYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKPEKLNFVKLG 733

Query: 721 EKLSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
           EKLSY+VSF  LG +E+  EFSFGSLVW SG YAVRSPIA
Sbjct: 734 EKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIA 765

BLAST of Cla004527 vs. NCBI nr
Match: gi|743788982|ref|XP_011035007.1| (PREDICTED: subtilisin-like protease [Populus euphratica])

HSP 1 Score: 990.7 bits (2560), Expect = 1.6e-285
Identity = 492/758 (64.91%), Postives = 592/758 (78.10%), Query Frame = 1

Query: 5   SSAAVDQQTYIIHMDTTKMVTT-----NPEQWYKAMIDSVNELSSLDDNEEEASNFAEIL 64
           S+A++D+QTYIIHMD  KM        N  QWY+++IDS+ + SS +  EE+ + F ++L
Sbjct: 18  SAASIDKQTYIIHMDKNKMPALYDPLGNSRQWYESVIDSITQFSSQEQEEEQETGFPQLL 77

Query: 65  YVYKTALSGKIFKCRCFAAKLSTKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRD 124
           Y Y+T  SG       FAAKLSTK++ +LS++ GFL+A P+ +L LHTTH+PQFLGLQ  
Sbjct: 78  YTYETVTSG-------FAAKLSTKQVEALSRVDGFLSAIPDGMLTLHTTHTPQFLGLQSG 137

Query: 125 HGLWNSSNMASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGPKFSPSNCNKRL 184
            GLWN+ N+ASD+I+G+LDTGIWPEH+SFQD G+  VP KWKG C++G KFSPSNCNK+L
Sbjct: 138 KGLWNAQNLASDVIVGILDTGIWPEHVSFQDSGMSAVPLKWKGKCESGTKFSPSNCNKKL 197

Query: 185 IGASAFIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKANFNNQGMGVATG 244
           IGA AF KGYE+I GR+NET  +RSPRDS GHGTHTA+TAAGN+V+KA+F     G A G
Sbjct: 198 IGARAFFKGYESIAGRINETVDYRSPRDSQGHGTHTAATAAGNLVDKASFYGLANGSAAG 257

Query: 245 MSFSSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFG 304
           M +++RI AYKVCW  GC N D+LAAMD AVADGVDVLSLSLGG A  FY D++AIA+FG
Sbjct: 258 MKYTARIAAYKVCWTSGCTNTDLLAAMDQAVADGVDVLSLSLGGSAKPFYSDSVAIASFG 317

Query: 305 AIQNGVFVSCSAGNSGPSSSTVGNAAPWIMTVAASYTDRTFPTTVKLGNRQVFEGSSLYY 364
           AIQ GVFVSCSAGNSGPS S+V N APWIMTVAASYTDR FPTTVKLGN Q FEG+SLY 
Sbjct: 318 AIQKGVFVSCSAGNSGPSISSVDNNAPWIMTVAASYTDRRFPTTVKLGNGQSFEGASLYT 377

Query: 365 GKNINELPLVYNNTAGDGQETNFCTAGSLDPSKVKGKIVLCERGSNSRTAKGEQVKLAGG 424
           GK   +LPLV+  TAG G+   +C  GSL    VKGK+V+C+RG + R  KGEQVKLAGG
Sbjct: 378 GKATAQLPLVHAGTAG-GEGAEYCITGSLKRKLVKGKMVVCKRGMSGRAQKGEQVKLAGG 437

Query: 425 AGMILINTQFEGEELFADPHVLPATTLGAKAGKAILDYIASSKSQAKASIAFQGTKYGSQ 484
            GM+LINT+  GEELFAD HVLPAT+LGA AG A+  Y+ S+K +A ASIAF+GT YG+ 
Sbjct: 438 TGMLLINTETGGEELFADAHVLPATSLGASAGMAVKGYMNSTK-RATASIAFKGTVYGNP 497

Query: 485 APRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTS 544
           AP +AAFSSRGPS VGPDVIKPDVTAPG+NILAAWPP+ SP+ L+SDKR VLFN+ISGTS
Sbjct: 498 APMLAAFSSRGPSSVGPDVIKPDVTAPGMNILAAWPPMTSPTLLKSDKRSVLFNVISGTS 557

Query: 545 MSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVSDNKMSLISDVGHASGGPADPFAFG 604
           MSCPHVSGLAALLKS HK WSPAAIKSALMTTAY +DN+ S I+D G ++   A PFAFG
Sbjct: 558 MSCPHVSGLAALLKSVHKKWSPAAIKSALMTTAYATDNRGSPIADAGSSNSASATPFAFG 617

Query: 605 SGHVNPEKASDPGLVYDIAPQDYINYLCSLKYNSTQIALVSRGKFTCSSKRTILQPGDLN 664
           SGHV+PE+ASDPGL+YDI  +DY+NY CSL Y S+QIA VSR   TC   +  LQPGDLN
Sbjct: 618 SGHVDPERASDPGLIYDITIEDYLNYFCSLDYTSSQIAQVSRRNVTCPDNKA-LQPGDLN 677

Query: 665 YPSFSVFMKKKAKNVTITLKRTVTNVGIPRSDYTVKINNPKGIAVIVKPEKLSFGSLGEK 724
           YPSF+V  +  A+N  +  KRT+TNVG P S Y VK+  P G++VI++P+ LSF  LG+K
Sbjct: 678 YPSFAVNFEGNARNNRVKYKRTLTNVGTPSSTYAVKVEEPNGVSVILEPKSLSFEKLGQK 737

Query: 725 LSYQVSFVGLGGKEASGEFSFGSLVWVSGKYAVRSPIA 758
           LSY V+FV  GGK   G  S GSLVW+SGKY+VRSPIA
Sbjct: 738 LSYNVTFVSSGGKGREGSSSIGSLVWLSGKYSVRSPIA 765