Cla003612 (gene) Watermelon (97103) v1

NameCla003612
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionMannose-6-phosphate isomerase (AHRD V1 **** B9SLX4_RICCO); contains Interpro domain(s) IPR016305 Mannose-6-phosphate isomerase
LocationChr1 : 5399405 .. 5402051 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGAGGAAGATGATAACAAATTGAAGAAGATTAGGAAGAAGAGACTGTCAGGCGCCGACAGGAGGAAGCGGCTCCTTCGCCTCAAATGTTGTGTTCAAAACTATGACTGGGGGGTTATTGGGTGTAATTCCCAAGTGGCGAGGCTGTTTTCGCTCAATTCAGGTTGCTGCAGTCTCGATCCGGGGGAATGTTACGCCGAATTTTGGATTGGGACGCATAAATCGGGCCCGTCTTTTGTTGGTTTTGGACGGGAAAATAATGCCGCCCCTGCGTTTGGATCCAAACCCCTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTTGGTGATAAAGTTGCGAGGAAATGGGGAGGGGATCTTCCCTTTCTTTTCAAGGTCCAATTTCTTTTCCATTAGATCCTTGGGAAATGTGTTTTGTCGGTGTGCTTGATTTCTTTCAAGATGTCACTATTGGACAGGAATTTTGGGTTTTAATTATGCTCTGCAATGTGTCGGTGTGTTAGTTAATATGGATGCTGAGAATTTACCTGTTGGATGACATTAATGCTTTATAAACGAAAATTATGTGCTGCAGCTCTTTGAATTGTATGTTCAATTAGTTCCTCGAACCTCAATTACCGTTCTTATTTTCCTTGATGTTAATGTTTTGAGCTCAGTTGGACTGCAACTTGATGATTTCTTATTGGGAGCATAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGCACGAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGATTTGAGGCCCTCTGTGGGTTCATCAGCTCCAAGGTACGACGACATGTAGTAGCAAAGTGTAGTTCTTCTGGGGTTTTTGTAAATTTTCTTGAACTAATTTTAAGCACAAATATCCTTGTTTTGCCTGTTGGTTAATTGAAAATATCTTATTGATTCTTACTCTGTTCGTATTATACACTAATTGGATTTAGAGAGATGAAAGAATCTCAAATCAAGATGTTGATTCAGCTTTTTGGTTAATCGTGCATGTATAAACTAACAATATAAGTAGCAGATAATGAATGAAGTTTCTTTATAAGTTCCTTTACTTTGCAAAACCCATGTCATTTGATTTTACTTATTTCATTATTTTCCAGGAGCTCAAGGCGGTGCTTAGTAGTGTTCCTGAGATAGTAGAATTGGTCCAATGTGCAGATGCAGAGAAGTTCTCACACGATAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTGGTCAGATCATGTCATCTAACAAGGGCATAGTTCGTGAGGCTATTAGTAAACTAAAAAGGCGTCTTAGTTTGGAGAAAAAGGTAACTGAAGTTTTATATCTCTACAAATTATCTTGTCAGCAGAATTTGGCATTTTTCATTGTTGACGTTGTTTTGTTTTAAATCTCAAGGCTTAAGGCTAACCATCATTACCAAGTTCTTTGATCGAAAGGATGTATTTCATAATAAAGCTTAAGTTGTTCTTTCTCACTTCATCAATCTCTGCAAAGTATTATTCATTTGCTTTCACATTGTTTTTTGCAACTTCTCATTGAATTGCATTCTTTTTTTCTTTTTCATCAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATACCCTGCTGATGTTGGTATACTAGCAGCATTTTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAACGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCTGGTCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGTAATCACAGAATGTATGCAGGAGCTGGTTTTATATAATGACGCAATTATAGTGTGTAATCTGATTATATGCTATTGGTTCTTGTTTTCCATGCCCTCTTTCTTGAAGTCCCCCTGCAGACTTGTATTTTCATATGAGTAGTTTATCTTTTCTTTCCCTTTTCTATTGCTTGCTAGCAGAAAGTAGTTAAAAATTGCCTTGGAAGTGCGATCTTGAACGACTTGATTCATATTGATCTACCTTTATTATCCATTGGGGATAGGGGTAGAAAGTAGAAAGACTAATGTGATTTTGAAGCACAATGTGAATTTCCACTCCAATCTGCCTTGTCCTACGCCTTGGAGTTTCACTGAGAAGATTGCTCATAATCAGTTCCTTCAACGTGCAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCCTTGAATGAATACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTCCCACAAGCTGCATCTGTGAGCTTCCCATCTGTCCCAGGACCATCTCTCTTTCTAGTGATGTCTGGAAAAGGGACAATTGTCACAGGCTATTCTGAAGAAAGTACATTTAAAGAGGGTGAAGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTATAACAGCTGCAGCTATAGAATTACATATGTACAGAGCTGGGGTCAACAACAGATTCTTTGGAGACTTGTGA

mRNA sequence

ATGGACGAGGAAGATGATAACAAATTGAAGAAGATTAGGAAGAAGAGACTGTCAGGCGCCGACAGGAGGAAGCGGCTCCTTCGCCTCAAATGTTGTGTTCAAAACTATGACTGGGGGGTTATTGGGTGTAATTCCCAAGTGGCGAGGCTGTTTTCGCTCAATTCAGGTTGCTGCAGTCTCGATCCGGGGGAATGTTACGCCGAATTTTGGATTGGGACGCATAAATCGGGCCCGTCTTTTGTTGGTTTTGGACGGGAAAATAATGCCGCCCCTGCGTTTGGATCCAAACCCCTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTTGGTGATAAAGTTGCGAGGAAATGGGGAGGGGATCTTCCCTTTCTTTTCAAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGCACGAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGATTTGAGGCCCTCTGTGGGTTCATCAGCTCCAAGGAGCTCAAGGCGGTGCTTAGTAGTGTTCCTGAGATAGTAGAATTGGTCCAATGTGCAGATGCAGAGAAGTTCTCACACGATAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTGGTCAGATCATGTCATCTAACAAGGGCATAGTTCGTGAGGCTATTAGTAAACTAAAAAGGCGTCTTAGTTTGGAGAAAAAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATACCCTGCTGATGTTGGTATACTAGCAGCATTTTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAACGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCTGGTCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCCTTGAATGAATACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTCCCACAAGCTGCATCTGTGAGCTTCCCATCTGTCCCAGGACCATCTCTCTTTCTAGTGATGTCTGGAAAAGGGACAATTGTCACAGGCTATTCTGAAGAAAGTACATTTAAAGAGGGTGAAGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTATAACAGCTGCAGCTATAGAATTACATATGTACAGAGCTGGGGTCAACAACAGATTCTTTGGAGACTTGTGA

Coding sequence (CDS)

ATGGACGAGGAAGATGATAACAAATTGAAGAAGATTAGGAAGAAGAGACTGTCAGGCGCCGACAGGAGGAAGCGGCTCCTTCGCCTCAAATGTTGTGTTCAAAACTATGACTGGGGGGTTATTGGGTGTAATTCCCAAGTGGCGAGGCTGTTTTCGCTCAATTCAGGTTGCTGCAGTCTCGATCCGGGGGAATGTTACGCCGAATTTTGGATTGGGACGCATAAATCGGGCCCGTCTTTTGTTGGTTTTGGACGGGAAAATAATGCCGCCCCTGCGTTTGGATCCAAACCCCTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTTGGTGATAAAGTTGCGAGGAAATGGGGAGGGGATCTTCCCTTTCTTTTCAAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGCACGAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGATTTGAGGCCCTCTGTGGGTTCATCAGCTCCAAGGAGCTCAAGGCGGTGCTTAGTAGTGTTCCTGAGATAGTAGAATTGGTCCAATGTGCAGATGCAGAGAAGTTCTCACACGATAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTGGTCAGATCATGTCATCTAACAAGGGCATAGTTCGTGAGGCTATTAGTAAACTAAAAAGGCGTCTTAGTTTGGAGAAAAAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATACCCTGCTGATGTTGGTATACTAGCAGCATTTTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAACGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCTGGTCTTACCTCTAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCCTTGAATGAATACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTCCCACAAGCTGCATCTGTGAGCTTCCCATCTGTCCCAGGACCATCTCTCTTTCTAGTGATGTCTGGAAAAGGGACAATTGTCACAGGCTATTCTGAAGAAAGTACATTTAAAGAGGGTGAAGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTATAACAGCTGCAGCTATAGAATTACATATGTACAGAGCTGGGGTCAACAACAGATTCTTTGGAGACTTGTGA

Protein sequence

MDEEDDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGECYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPAYMEVSITAAAIELHMYRAGVNNRFFGDL
BLAST of Cla003612 vs. Swiss-Prot
Match: MPI1_ARATH (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana GN=PMI1 PE=1 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 7.1e-128
Identity = 235/428 (54.91%), Postives = 313/428 (73.13%), Query Frame = 1

Query: 20  ADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGECYAEFWIGTHKSGPS 79
           ADRR RL RL+C V++YDWG IG +S V R+++ NS    +DP   YAE W+GTH+SGPS
Sbjct: 15  ADRR-RLRRLRCSVKDYDWGKIGSDSLVYRVYAANSDY-EIDPTRPYAELWMGTHESGPS 74

Query: 80  FVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSI 139
           ++          A GS  ++L+ WI+ +P + LG++V  KWG DLPFLFKVLS+ + LSI
Sbjct: 75  YL--------EDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLSI 134

Query: 140 QAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKAVLSSVPEIVE 199
           QAHPDK LA+ +H+A P++YKDDNHKPEMALA T FEALCGFI  +ELK+V+ ++PEI E
Sbjct: 135 QAHPDKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEE 194

Query: 200 LVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKRRLSLEKKKRQ 259
           LV   +A +    +E D +EKVK +  +IF  +MS++    ++ +SKLKRRL +E ++RQ
Sbjct: 195 LVGSEEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQ 254

Query: 260 LSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATS 319
           L+ KE+L+L+LE QYP D+G+++AFF NYV+L PGEALY+G NEPHAY+ GEC+E MATS
Sbjct: 255 LTDKERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATS 314

Query: 320 DNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQ 379
           DNVVRAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP 
Sbjct: 315 DNVVRAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPS 374

Query: 380 AASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPAYMEVSITAAAIELHMY 439
            AS  FPSVPGPSL LV+ G+G + T  S +     G+VLFVPA  E+ + +++ +L +Y
Sbjct: 375 GASTVFPSVPGPSLLLVLQGEGRMSTEASADG-ISMGDVLFVPADTEIHLRSSS-DLKLY 428

Query: 440 RAGVNNRF 448
           RAG+N+RF
Sbjct: 435 RAGINSRF 428

BLAST of Cla003612 vs. Swiss-Prot
Match: MPI2_ARATH (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana GN=PMI2 PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.5e-127
Identity = 230/428 (53.74%), Postives = 303/428 (70.79%), Query Frame = 1

Query: 24  KRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGECYAEFWIGTHKSGPSFVGF 83
           + + RL+C V+NY+WG +G  S VARL   N+G   +D    YAEFW+GTH+SGPS V F
Sbjct: 20  EEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQ-RVDSEIPYAEFWMGTHESGPSHVEF 79

Query: 84  GRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHP 143
           G  +  +       ++LK W+  +P  +LG KV  KWG DLPFLFKVLS+ KALSIQAHP
Sbjct: 80  GSGHGVSDKC---MVTLKSWVLDNPN-LLGSKVVDKWGCDLPFLFKVLSVTKALSIQAHP 139

Query: 144 DKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKAVLSSVPEIVELVQC 203
           +K LA  LH   P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV  
Sbjct: 140 NKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVGS 199

Query: 204 ADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKRRLSLEKKKRQLSAK 263
             A++  +  E D  E++K +   IF Q+MS++    ++ +S++K RL LE K R+LS K
Sbjct: 200 KAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSEK 259

Query: 264 EQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVV 323
           E+L+L LE QY  D+G+++AFF NYV+L PGEALY+  NEPHAYISG+C+ECMA SDNVV
Sbjct: 260 EKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNVV 319

Query: 324 RAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAASV 383
           RAGLT K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP+  S 
Sbjct: 320 RAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKST 379

Query: 384 SFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPAYMEVSITAAAIELHMYRAGV 443
            FP+VPGPS++LV+ GKG + TG S +     G+VLFVPA +E+ +T  +  + +YRAGV
Sbjct: 380 VFPAVPGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAGV 439

Query: 444 NNRFFGDL 452
           ++RFF  L
Sbjct: 440 SSRFFQTL 441

BLAST of Cla003612 vs. Swiss-Prot
Match: MPI_DICDI (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum GN=mpi PE=3 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 8.2e-76
Identity = 178/455 (39.12%), Postives = 254/455 (55.82%), Query Frame = 1

Query: 21  DRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGEC--YAEFWIGTHKSGP 80
           +    ++ LKC  QNY+WG  G NS VA+L    +  CS    E   YAE W+G H S P
Sbjct: 16  NNNNNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAP 75

Query: 81  SFVGFGRENNAAPAFGSKPLSLKDWI--------------SVDPGAVLGDKVARKWGGDL 140
           S V           + +K L L+++I              S    ++ G+ V +++G D 
Sbjct: 76  SKV----------EYKNKELKLREYIDTVQKEINEKSSTSSSSSSSIRGEIVEKRFGNDF 135

Query: 141 PFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISS 200
           PFLFKVLSI  ALSIQAHPD  LA+ L +  P+IYKD  HKPE+A+A T FEALC F   
Sbjct: 136 PFLFKVLSIRTALSIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPL 195

Query: 201 KELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAI 260
            E+++ + ++PE           K S  +     +  K+  +SI   ++ ++  ++   +
Sbjct: 196 SEIQSFIDTIPEF----------KNSLPNNLIKLDDCKEYLKSIVTSLLKADGLLISNNL 255

Query: 261 SKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEP 320
            +L  RL+ EK++ +    ++L+L+L  QYP DVG+  A+ LNY+ LKPGEAL++G  EP
Sbjct: 256 KELNNRLN-EKREEERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEP 315

Query: 321 HAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL-----QGVSLNEY 380
           HAYISG+C+ECMA SDNVVRAGLT K +DV  L  ML Y+ G P+++       +SL  Y
Sbjct: 316 HAYISGDCVECMAPSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLVVPQQRPDLSLTNY 375

Query: 381 TRKYAPPFDEFEVDRCILPQAA-SVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKE--- 440
            R Y PP DEF+V+   L     +++  S  GPS+ LV SG  +I    S +ST      
Sbjct: 376 -RCYQPPVDEFQVEYYHLDDCCKNINVYSSKGPSIVLVYSGNLSI-ENKSNQSTLNNLHT 435

Query: 441 GEVLFVPAYMEVSI--TAAAIELHMYRAGVNNRFF 449
           G +LFVPA  +     + ++I + +Y A V+NR F
Sbjct: 436 GSILFVPANTDYQFIQSDSSIPVSIYVASVSNRIF 447

BLAST of Cla003612 vs. Swiss-Prot
Match: MPI_CRYNJ (Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) GN=MAN1 PE=3 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 1.2e-74
Identity = 181/437 (41.42%), Postives = 238/437 (54.46%), Query Frame = 1

Query: 33  VQNYDWGVIGCNSQVARLFSLNSGCCSLDPGECYAEFWIGTHKSGPSFVGFGRENNAAPA 92
           + +YDWG  G  S  A+L + +    S+D  + YAE W+GTH + PS +           
Sbjct: 12  INSYDWGKKGSASLAAQLATTSIPDFSIDEDKAYAELWMGTHPNNPSRL----------- 71

Query: 93  FGSKPLSLKDWISVDPGAVLGDKVARKW----GGDLPFLFKVLSIEKALSIQAHPDKDLA 152
             S    L + +   P  ++G  V+ K+     G LPFLFKVLSI  ALSIQAHPDK LA
Sbjct: 72  --SDNTLLSEHLKSHP-ELIGSSVSSKFEDCKDGSLPFLFKVLSIGTALSIQAHPDKPLA 131

Query: 153 RSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKAVLSSVPEIVELVQCADAEK 212
           + L + +P +YKD NHKPEMA+ALT F A   F+    L   L +VPE+ E V  +  E 
Sbjct: 132 KKLFDEKPDVYKDPNHKPEMAIALTPFLAFLNFLPLSVLLLHLLTVPELQEFVDSSLTES 191

Query: 213 FSHD----------------SEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKRRLS 272
            +                  +E     + K + + IF  +MS++K +V EAISKL +R  
Sbjct: 192 LASSLGLPTSQPPDTSLFKPTESPATAEQKDILKQIFAALMSADKKLVEEAISKLIKRYQ 251

Query: 273 LEKK-KRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGE 332
            ++  K    +   L LRL  QYP DVG+L  F LN VELK GEA ++G NEPHAYI G+
Sbjct: 252 AKRDIKENEKSLVDLALRLNDQYPGDVGVLCVFLLNVVELKRGEAAFLGANEPHAYIEGD 311

Query: 333 CIECMATSDNVVRAGLTSKKRDVQALLSMLNYK--QGFPEILQGVSL---NEYTRKYAPP 392
            IECMATSDNVVRAGLT K RDV  L+SML Y+   G  ++LQ       ++ T+ Y PP
Sbjct: 312 IIECMATSDNVVRAGLTPKLRDVDTLVSMLTYEAAPGNKQLLQPTPFQKGDDTTKLYDPP 371

Query: 393 FDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPAYME 444
             EF V R  L +    S   V GPSL ++  G+G +  G ++ S F  G+V+FV A  E
Sbjct: 372 IAEFSVLRTELSKGMKTSHRPVEGPSLCVITEGEGVVRNG-NDRSEFVRGDVIFVGAGKE 431

BLAST of Cla003612 vs. Swiss-Prot
Match: MPI_ECHMU (Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis GN=PMIH PE=2 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 3.2e-72
Identity = 180/451 (39.91%), Postives = 245/451 (54.32%), Query Frame = 1

Query: 26  LLRLKCCVQNYDWGVIGCNSQVARL--FSLNSGCCSLDPGECYAEFWIGTHKSGPSFVGF 85
           +LRLKC  Q YDWG +G NS V +L   S N     +D    YAE W+GTH SG S +  
Sbjct: 1   MLRLKCASQRYDWGKVGVNSIVYKLQVASENPDISHVDLP--YAELWMGTHPSGSSCL-- 60

Query: 86  GRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHP 145
                    + +  ++L   I  +P A LG      +G  LPFLFKVLS+ KALSIQAHP
Sbjct: 61  ---------WDNPHVTLAAHIQENP-ASLGKPSLIYFGRRLPFLFKVLSVAKALSIQAHP 120

Query: 146 DKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKAVLSSVPEIVEL--- 205
           DK +A  LH  QP +YKD +HKPEMA+ALT FEAL GF    ++ A + + PE+ EL   
Sbjct: 121 DKKMAVRLHAEQPDLYKDGSHKPEMAIALTDFEALLGFRPLNQILAFIQAFPELAELTTL 180

Query: 206 ----------VQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKRR 265
                     VQ    ++   +  +   EKV+   +S+  +  + +K +    +S L   
Sbjct: 181 ELPSPEEKRDVQPPSIKQLYSNLMRSSPEKVESTIKSLLNRFTTGSKSVCDPPLSIL--- 240

Query: 266 LSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISG 325
             ++ ++  + A   L LRL   +P DVG L+ FFLNY+ LK GEA+++  N PHAY+SG
Sbjct: 241 -GVDLQEEDVQALVDLFLRLTQAFPGDVGCLSIFFLNYIRLKSGEAIFLKANTPHAYLSG 300

Query: 326 ECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT------RKYA 385
           +C+ECMA SDNVVRAGLT K +DV+ LL ML+Y      +  G +    T      + + 
Sbjct: 301 DCVECMANSDNVVRAGLTPKFKDVERLLEMLDYTPLTDSLRLGATQPIPTPEGISMKSFI 360

Query: 386 PPFDEFEVDRCIL-PQAASVSFPSVPGPSLFLVMSGKGTIV---TG--YSEESTFKEGEV 445
           PP  EF VD      ++   S PSVP  S+ L + G+GTI    TG   S+E+ F  G V
Sbjct: 361 PPVSEFAVDVIQFDAESRGFSLPSVPTASILLFLHGQGTITCPSTGEACSQETKFGPGFV 420

Query: 446 LFVPAYMEVSITAAAI---ELHMYRAGVNNR 447
            FVPA M V++ +       LH +RA VN +
Sbjct: 421 YFVPADMIVNLVSKEDRKGSLHAFRAYVNRK 433

BLAST of Cla003612 vs. TrEMBL
Match: A0A0A0KL16_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G167200 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 2.5e-241
Identity = 421/451 (93.35%), Postives = 434/451 (96.23%), Query Frame = 1

Query: 1   MDEEDDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSL 60
           MDEEDD KL+K RKKRL GADRRKRLLRLKCCVQNYDWGVIGCNSQVARLF LNSG C++
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPGECYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DPGECYAEFWIGTHKSGPSFV +GR+NNA  AFGSKPLSLKD ISVDP AVLGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSL+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIV 240
           FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIF QIMSSNKGIV
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAISKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REA+ KLKRRLSLEKKKRQLSAKEQLILRLE QYPADVGILAA+FLNYVELKPGEALYVG
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTI+TGYSEE+T +EGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           VPAYMEVSITA AIELHMYRAG+NNRFF DL
Sbjct: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of Cla003612 vs. TrEMBL
Match: A0A067JZM8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18357 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.0e-158
Identity = 290/448 (64.73%), Postives = 351/448 (78.35%), Query Frame = 1

Query: 4   EDDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPG 63
           +DD K K++ K+        K+L RLKC VQNYDWG+IG NSQVARLF+LNSG   + P 
Sbjct: 22  DDDPKEKQLLKQN-------KKLPRLKCSVQNYDWGIIGNNSQVARLFALNSGS-DICPN 81

Query: 64  ECYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGD 123
           + YAEFWIGTH+SGPSF+  G        F S  LSLK WI  +P  VLGD+V  KWGGD
Sbjct: 82  KPYAEFWIGTHESGPSFLDHGNAG-----FRSS-LSLKSWILDNPILVLGDRVFEKWGGD 141

Query: 124 LPFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFIS 183
           LPFL KVLS+EKALSIQAHPDK+LAR+LH +QP++YKD NHKPEMALALT FEALCGFIS
Sbjct: 142 LPFLLKVLSVEKALSIQAHPDKELARALHISQPNVYKDGNHKPEMALALTEFEALCGFIS 201

Query: 184 SKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREA 243
           +KELK VL +VPE+ ELV  ++AE+F + SEQD   KVK + E IF Q++SS++  + E 
Sbjct: 202 TKELKDVLRNVPELEELVGGSNAEQFLYFSEQDRDAKVKAILEYIFSQLLSSSREKIFEV 261

Query: 244 ISKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNE 303
           ISK+KRRL LE ++R+L+ KEQL+LRLE QYPAD G++AAF LNYV+L  GEALY+G NE
Sbjct: 262 ISKIKRRLHLEMQERELTDKEQLVLRLESQYPADTGVIAAFLLNYVKLNRGEALYLGANE 321

Query: 304 PHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKY 363
           PHAYI+GECIECMA SDNVVR GLTSK+RD+Q LL+ML Y+QGFPEILQGVSLN YT +Y
Sbjct: 322 PHAYIAGECIECMANSDNVVRVGLTSKRRDIQTLLAMLEYRQGFPEILQGVSLNPYTTRY 381

Query: 364 APPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPA 423
            PP DEFEVD  ++P+AASV FPSVPGPS+ L + G+GTI  G+S++    EGEVLFVPA
Sbjct: 382 LPPLDEFEVDLSVIPRAASVVFPSVPGPSVLLFIMGRGTIAAGFSKDGKAGEGEVLFVPA 441

Query: 424 YMEVSITAAAIELHMYRAGVNNRFFGDL 452
             E+ ITA + ELH+YRAGVN+RFF DL
Sbjct: 442 CTEIRITAESAELHLYRAGVNSRFFEDL 455

BLAST of Cla003612 vs. TrEMBL
Match: B9SLX4_RICCO (Mannose-6-phosphate isomerase OS=Ricinus communis GN=RCOM_1309320 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.1e-156
Identity = 280/423 (66.19%), Postives = 334/423 (78.96%), Query Frame = 1

Query: 28  RLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGECYAEFWIGTHKSGPSFVGFGREN 87
           RLKC V NYDWG++G NSQVARLFSLNSG   +DPG+ YAEFWIGTHKSGPSFV  G  N
Sbjct: 30  RLKCSVHNYDWGIVGKNSQVARLFSLNSGS-DIDPGKPYAEFWIGTHKSGPSFVDHGNGN 89

Query: 88  NAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHPDKDL 147
            +        LSLK WI  +P  VLGDKV +KWGGDLPFLFKV+S+ KALSIQAHPDK+L
Sbjct: 90  GS--------LSLKKWILENPYKVLGDKVMKKWGGDLPFLFKVISVGKALSIQAHPDKEL 149

Query: 148 ARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKAVLSSVPEIVELVQCADAE 207
           A++LH+AQPSIYKDDNHKPEMALALT FEALCGFIS KELK +L + PEIVELV  ++ +
Sbjct: 150 AKALHKAQPSIYKDDNHKPEMALALTEFEALCGFISVKELKNMLCTFPEIVELVGDSNVK 209

Query: 208 KFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKRRLSLEKKKRQLSAKEQLI 267
           +F H +EQD  +K K   +SIF QI+ S+   + E IS +KRRL LE ++R+L+ KE L+
Sbjct: 210 QFLHMNEQDRDDKGKTFLQSIFSQIVLSSSDEICELISNMKRRLHLEMQERELTDKEWLV 269

Query: 268 LRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVVRAGL 327
           LRLE QYPADVG++AAF LNYV+L PGEALY+  NEPHAY+ GECIECMA SDNVVRAGL
Sbjct: 270 LRLESQYPADVGVMAAFLLNYVKLSPGEALYLEANEPHAYVGGECIECMANSDNVVRAGL 329

Query: 328 TSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAASVSFPS 387
           T K+RDVQ LLSML Y+QGFPEIL+GV L+ +T +Y PP DEFEVD  ILP+AASV FPS
Sbjct: 330 TPKQRDVQTLLSMLKYRQGFPEILRGVPLSPFTTRYRPPLDEFEVDLSILPEAASVVFPS 389

Query: 388 VPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPAYMEVSITAAAIELHMYRAGVNNRF 447
           +PGPSLFL++ G+GTI  G+ +    KEGE  FVPAY E+ ITA + +LH+YRAGVN+RF
Sbjct: 390 IPGPSLFLIIMGRGTIDAGFCDGKIVKEGEAYFVPAYTEIRITAKSTKLHLYRAGVNSRF 443

Query: 448 FGD 451
             D
Sbjct: 450 LKD 443

BLAST of Cla003612 vs. TrEMBL
Match: A0A067GNE7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g014147mg PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 2.6e-153
Identity = 283/448 (63.17%), Postives = 349/448 (77.90%), Query Frame = 1

Query: 1   MDEEDDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSL 60
           M E ++ ++KK RK+ ++      + LRL+C V+NY+WG++G  S V+RLF LNSG   +
Sbjct: 1   MAENNNTEIKK-RKRMIN------KPLRLQCAVKNYNWGIVGSKSHVSRLFCLNSGT-HI 60

Query: 61  DPGECYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           +P E YAEFWIGTH+SG SF+  G             LSLK+WIS +P  VLGD+V  KW
Sbjct: 61  NPNEPYAEFWIGTHESGASFLDHGG------------LSLKEWISKNPD-VLGDRVLNKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+LH++QPS+Y D+NHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARTLHKSQPSLYSDENHKPEMALALTEFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIV 240
           FIS KEL+ VL +VPEIV+LV  ADAE+    +E    ++VK + ESIF QI+ S+K  +
Sbjct: 181 FISLKELRNVLCTVPEIVDLVGGADAEQCFPVNEFGRSQEVKAIVESIFSQILLSSKDEI 240

Query: 241 REAISKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
            E IS+LK RL+LEKKKRQL+ KE L+LRLEGQYP D G+LAAF LNYV+L  GEAL +G
Sbjct: 241 CEIISRLKWRLNLEKKKRQLTDKEMLVLRLEGQYPDDAGVLAAFLLNYVKLNRGEALCIG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
            NEPHAYI GECIECMATSDNVVRAGLTSK RD+Q L SMLN +QGFP+IL+G+SLN YT
Sbjct: 301 ANEPHAYIRGECIECMATSDNVVRAGLTSKHRDIQTLFSMLNRRQGFPQILKGISLNPYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLF 420
            +Y PPF+EFEVD C++PQAAS+ FPSV GPSLFL +SG GT+  G+S+E   +EGEVLF
Sbjct: 361 TRYLPPFEEFEVDCCVIPQAASLVFPSVAGPSLFLFISGNGTLSAGFSKEQIVEEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFF 449
           VPAYME +I + + ELH+YRAGVN+ FF
Sbjct: 421 VPAYMEFTIASQSKELHLYRAGVNSSFF 427

BLAST of Cla003612 vs. TrEMBL
Match: M5XC42_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005896mg PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 7.8e-150
Identity = 283/449 (63.03%), Postives = 337/449 (75.06%), Query Frame = 1

Query: 10  KKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGECYAEF 69
           K ++KKR     R +R +RL+C V+NYDWG++G +S+VARLF+LNSG   +DP + YAEF
Sbjct: 6   KNMKKKR----KRSRRPVRLRCSVKNYDWGIVGRHSKVARLFALNSGS-DIDPAKPYAEF 65

Query: 70  WIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFK 129
           WIGTH+SGPS+   G          S+P+SLK WI+ DP +VLGDKV  KWG DLPFLFK
Sbjct: 66  WIGTHESGPSYADSG----------SEPVSLKAWIAQDP-SVLGDKVVDKWGADLPFLFK 125

Query: 130 VLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKA 189
           VLS+ KALSIQAHPDK+LAR LH+  P++Y+DDNHKPEMALALT FEALCGFIS KELK 
Sbjct: 126 VLSVGKALSIQAHPDKELARVLHKLYPNVYRDDNHKPEMALALTEFEALCGFISVKELKD 185

Query: 190 VLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKR 249
           +LSSVPEI ELV   +AE+    +EQ    K K   ESIF ++M  +K  + E ISKLKR
Sbjct: 186 MLSSVPEIAELVGVGNAERILLVNEQHENGKSKTDLESIFSRVMLLSKDTISEMISKLKR 245

Query: 250 RLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYIS 309
           RL+LEKKKRQL+ KEQL LRLE QYPADVG +AAFFLNYV+L  GEA+ +GPNEPHAYIS
Sbjct: 246 RLNLEKKKRQLTVKEQLALRLESQYPADVGAIAAFFLNYVKLNRGEAISLGPNEPHAYIS 305

Query: 310 GECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDE 369
           GECIECMATSDNVVRAGLTSK  DVQ LLSML Y+QG PEILQGV LN YT +Y PPF+E
Sbjct: 306 GECIECMATSDNVVRAGLTSKPLDVQTLLSMLKYRQGSPEILQGVPLNPYTTRYLPPFEE 365

Query: 370 FEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFK-------EGEVLFVP 429
           FE+D C LPQ+A+V FPSV GPSLFL  +GKG    G  E+   K       EGEV FVP
Sbjct: 366 FEIDCCNLPQSAAVIFPSVVGPSLFLFTAGKGHFDAGLPEDDMVKEGDDIVEEGEVFFVP 425

Query: 430 AYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           A  ++ ITA + EL +YR GVN++ + DL
Sbjct: 426 ANTKIIITAKSTELQLYRVGVNSKIYRDL 438

BLAST of Cla003612 vs. NCBI nr
Match: gi|449451854|ref|XP_004143675.1| (PREDICTED: mannose-6-phosphate isomerase 1 [Cucumis sativus])

HSP 1 Score: 842.4 bits (2175), Expect = 3.6e-241
Identity = 421/451 (93.35%), Postives = 434/451 (96.23%), Query Frame = 1

Query: 1   MDEEDDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSL 60
           MDEEDD KL+K RKKRL GADRRKRLLRLKCCVQNYDWGVIGCNSQVARLF LNSG C++
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPGECYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DPGECYAEFWIGTHKSGPSFV +GR+NNA  AFGSKPLSLKD ISVDP AVLGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSL+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIV 240
           FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIF QIMSSNKGIV
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAISKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REA+ KLKRRLSLEKKKRQLSAKEQLILRLE QYPADVGILAA+FLNYVELKPGEALYVG
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTI+TGYSEE+T +EGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           VPAYMEVSITA AIELHMYRAG+NNRFF DL
Sbjct: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of Cla003612 vs. NCBI nr
Match: gi|659073250|ref|XP_008436962.1| (PREDICTED: mannose-6-phosphate isomerase 1-like [Cucumis melo])

HSP 1 Score: 642.5 bits (1656), Expect = 5.5e-181
Identity = 323/352 (91.76%), Postives = 333/352 (94.60%), Query Frame = 1

Query: 1   MDEEDDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSL 60
           MDEEDD K KK RKKRL GADRRKRLLRLKCCVQNYDWGVIGCNSQVARLF LNSG C++
Sbjct: 1   MDEEDDKKFKKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGRCNV 60

Query: 61  DPGECYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DPGECYAEFWIGTHKSGPS+V +GR+NNAA AFGSKPLSLKD IS+DP A+LGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSYVVYGRDNNAAAAFGSKPLSLKDCISLDPVALLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSL+EAQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIV 240
           FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIF QIMSSNKG+V
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGLV 240

Query: 241 REAISKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REA+ KLKRRLSLEKKKRQLSAKEQLILRLE QYPADVGILAAFFLNYVELKPGEALYVG
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAFFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQ 353
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQ     LQ
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQVITYCLQ 352

BLAST of Cla003612 vs. NCBI nr
Match: gi|1009133552|ref|XP_015883964.1| (PREDICTED: mannose-6-phosphate isomerase 2-like [Ziziphus jujuba])

HSP 1 Score: 581.6 bits (1498), Expect = 1.2e-162
Identity = 297/447 (66.44%), Postives = 353/447 (78.97%), Query Frame = 1

Query: 5   DDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGE 64
           DD++L  ++K+R S     K  LRL+C VQNYDWGV+G +S VA L +LNSG  ++D  +
Sbjct: 2   DDDELINVKKRRKSRISNNKTALRLRCSVQNYDWGVLGNHSHVATLSALNSGS-AIDSDK 61

Query: 65  CYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDL 124
            YAE WIGTH+SGPSFV     + +     S+ ++LKDWIS D  +VLGDKVA KWGGDL
Sbjct: 62  PYAEIWIGTHESGPSFVDCC--SLSGDELDSEMVTLKDWISEDL-SVLGDKVADKWGGDL 121

Query: 125 PFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISS 184
           PFLFKVL+I KA+SIQ HPDKD AR LH+ QPS+YKDDNHKPEMALALT FEALCGFIS 
Sbjct: 122 PFLFKVLAIGKAVSIQVHPDKDFARVLHKLQPSVYKDDNHKPEMALALTEFEALCGFISV 181

Query: 185 KELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAI 244
           KELK +L SVPEIVE V   +AE+  H  EQD   KVK++ ESIF ++M  +K  + E I
Sbjct: 182 KELKDILGSVPEIVEFVGITEAEQILHIIEQDENGKVKEVLESIFNKLMLLDKDTITEMI 241

Query: 245 SKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEP 304
            K+KRRL+ EKKKRQL+ KE ++LRLE QYPADVGI+AAFFLNYV+L  GEAL+VGPNEP
Sbjct: 242 YKIKRRLNTEKKKRQLTVKEHVVLRLESQYPADVGIIAAFFLNYVKLNGGEALFVGPNEP 301

Query: 305 HAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYA 364
           HAY+SGECIECMATSDNVVRAGLT K RDV+ LLSML Y+QGFPEIL+GV LN+YT+KY 
Sbjct: 302 HAYLSGECIECMATSDNVVRAGLTPKHRDVRTLLSMLKYRQGFPEILRGVPLNQYTKKYL 361

Query: 365 PPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPAY 424
           PP DEFEVDRC+LPQAAS  FPSVPGPSLFL +SGKG   TG SE++  + GE+LFVPA 
Sbjct: 362 PPCDEFEVDRCLLPQAASAVFPSVPGPSLFLFISGKGKFDTGSSEDAIVEGGEILFVPAS 421

Query: 425 MEVSITAAAIELHMYRAGVNNRFFGDL 452
           M+VSITA + ELH+YRAGVN+R F DL
Sbjct: 422 MKVSITAESTELHLYRAGVNSRLFRDL 444

BLAST of Cla003612 vs. NCBI nr
Match: gi|802681488|ref|XP_012082118.1| (PREDICTED: mannose-6-phosphate isomerase 1-like [Jatropha curcas])

HSP 1 Score: 567.0 bits (1460), Expect = 2.9e-158
Identity = 290/448 (64.73%), Postives = 351/448 (78.35%), Query Frame = 1

Query: 4   EDDNKLKKIRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPG 63
           +DD K K++ K+        K+L RLKC VQNYDWG+IG NSQVARLF+LNSG   + P 
Sbjct: 22  DDDPKEKQLLKQN-------KKLPRLKCSVQNYDWGIIGNNSQVARLFALNSGS-DICPN 81

Query: 64  ECYAEFWIGTHKSGPSFVGFGRENNAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGD 123
           + YAEFWIGTH+SGPSF+  G        F S  LSLK WI  +P  VLGD+V  KWGGD
Sbjct: 82  KPYAEFWIGTHESGPSFLDHGNAG-----FRSS-LSLKSWILDNPILVLGDRVFEKWGGD 141

Query: 124 LPFLFKVLSIEKALSIQAHPDKDLARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFIS 183
           LPFL KVLS+EKALSIQAHPDK+LAR+LH +QP++YKD NHKPEMALALT FEALCGFIS
Sbjct: 142 LPFLLKVLSVEKALSIQAHPDKELARALHISQPNVYKDGNHKPEMALALTEFEALCGFIS 201

Query: 184 SKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREA 243
           +KELK VL +VPE+ ELV  ++AE+F + SEQD   KVK + E IF Q++SS++  + E 
Sbjct: 202 TKELKDVLRNVPELEELVGGSNAEQFLYFSEQDRDAKVKAILEYIFSQLLSSSREKIFEV 261

Query: 244 ISKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNE 303
           ISK+KRRL LE ++R+L+ KEQL+LRLE QYPAD G++AAF LNYV+L  GEALY+G NE
Sbjct: 262 ISKIKRRLHLEMQERELTDKEQLVLRLESQYPADTGVIAAFLLNYVKLNRGEALYLGANE 321

Query: 304 PHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKY 363
           PHAYI+GECIECMA SDNVVR GLTSK+RD+Q LL+ML Y+QGFPEILQGVSLN YT +Y
Sbjct: 322 PHAYIAGECIECMANSDNVVRVGLTSKRRDIQTLLAMLEYRQGFPEILQGVSLNPYTTRY 381

Query: 364 APPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPA 423
            PP DEFEVD  ++P+AASV FPSVPGPS+ L + G+GTI  G+S++    EGEVLFVPA
Sbjct: 382 LPPLDEFEVDLSVIPRAASVVFPSVPGPSVLLFIMGRGTIAAGFSKDGKAGEGEVLFVPA 441

Query: 424 YMEVSITAAAIELHMYRAGVNNRFFGDL 452
             E+ ITA + ELH+YRAGVN+RFF DL
Sbjct: 442 CTEIRITAESAELHLYRAGVNSRFFEDL 455

BLAST of Cla003612 vs. NCBI nr
Match: gi|255572106|ref|XP_002526993.1| (PREDICTED: mannose-6-phosphate isomerase 1 [Ricinus communis])

HSP 1 Score: 561.2 bits (1445), Expect = 1.6e-156
Identity = 280/423 (66.19%), Postives = 334/423 (78.96%), Query Frame = 1

Query: 28  RLKCCVQNYDWGVIGCNSQVARLFSLNSGCCSLDPGECYAEFWIGTHKSGPSFVGFGREN 87
           RLKC V NYDWG++G NSQVARLFSLNSG   +DPG+ YAEFWIGTHKSGPSFV  G  N
Sbjct: 30  RLKCSVHNYDWGIVGKNSQVARLFSLNSGS-DIDPGKPYAEFWIGTHKSGPSFVDHGNGN 89

Query: 88  NAAPAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHPDKDL 147
            +        LSLK WI  +P  VLGDKV +KWGGDLPFLFKV+S+ KALSIQAHPDK+L
Sbjct: 90  GS--------LSLKKWILENPYKVLGDKVMKKWGGDLPFLFKVISVGKALSIQAHPDKEL 149

Query: 148 ARSLHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKAVLSSVPEIVELVQCADAE 207
           A++LH+AQPSIYKDDNHKPEMALALT FEALCGFIS KELK +L + PEIVELV  ++ +
Sbjct: 150 AKALHKAQPSIYKDDNHKPEMALALTEFEALCGFISVKELKNMLCTFPEIVELVGDSNVK 209

Query: 208 KFSHDSEQDGKEKVKQLFESIFGQIMSSNKGIVREAISKLKRRLSLEKKKRQLSAKEQLI 267
           +F H +EQD  +K K   +SIF QI+ S+   + E IS +KRRL LE ++R+L+ KE L+
Sbjct: 210 QFLHMNEQDRDDKGKTFLQSIFSQIVLSSSDEICELISNMKRRLHLEMQERELTDKEWLV 269

Query: 268 LRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVVRAGL 327
           LRLE QYPADVG++AAF LNYV+L PGEALY+  NEPHAY+ GECIECMA SDNVVRAGL
Sbjct: 270 LRLESQYPADVGVMAAFLLNYVKLSPGEALYLEANEPHAYVGGECIECMANSDNVVRAGL 329

Query: 328 TSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAASVSFPS 387
           T K+RDVQ LLSML Y+QGFPEIL+GV L+ +T +Y PP DEFEVD  ILP+AASV FPS
Sbjct: 330 TPKQRDVQTLLSMLKYRQGFPEILRGVPLSPFTTRYRPPLDEFEVDLSILPEAASVVFPS 389

Query: 388 VPGPSLFLVMSGKGTIVTGYSEESTFKEGEVLFVPAYMEVSITAAAIELHMYRAGVNNRF 447
           +PGPSLFL++ G+GTI  G+ +    KEGE  FVPAY E+ ITA + +LH+YRAGVN+RF
Sbjct: 390 IPGPSLFLIIMGRGTIDAGFCDGKIVKEGEAYFVPAYTEIRITAKSTKLHLYRAGVNSRF 443

Query: 448 FGD 451
             D
Sbjct: 450 LKD 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MPI1_ARATH7.1e-12854.91Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana GN=PMI1 PE=1 SV=1[more]
MPI2_ARATH3.5e-12753.74Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana GN=PMI2 PE=1 SV=1[more]
MPI_DICDI8.2e-7639.12Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum GN=mpi PE=3 S... [more]
MPI_CRYNJ1.2e-7441.42Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotyp... [more]
MPI_ECHMU3.2e-7239.91Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis GN=PMIH PE... [more]
Match NameE-valueIdentityDescription
A0A0A0KL16_CUCSA2.5e-24193.35Uncharacterized protein OS=Cucumis sativus GN=Csa_5G167200 PE=3 SV=1[more]
A0A067JZM8_JATCU2.0e-15864.73Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18357 PE=3 SV=1[more]
B9SLX4_RICCO1.1e-15666.19Mannose-6-phosphate isomerase OS=Ricinus communis GN=RCOM_1309320 PE=3 SV=1[more]
A0A067GNE7_CITSI2.6e-15363.17Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g014147mg PE=3 SV=1[more]
M5XC42_PRUPE7.8e-15063.03Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005896mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
gi|449451854|ref|XP_004143675.1|3.6e-24193.35PREDICTED: mannose-6-phosphate isomerase 1 [Cucumis sativus][more]
gi|659073250|ref|XP_008436962.1|5.5e-18191.76PREDICTED: mannose-6-phosphate isomerase 1-like [Cucumis melo][more]
gi|1009133552|ref|XP_015883964.1|1.2e-16266.44PREDICTED: mannose-6-phosphate isomerase 2-like [Ziziphus jujuba][more]
gi|802681488|ref|XP_012082118.1|2.9e-15864.73PREDICTED: mannose-6-phosphate isomerase 1-like [Jatropha curcas][more]
gi|255572106|ref|XP_002526993.1|1.6e-15666.19PREDICTED: mannose-6-phosphate isomerase 1 [Ricinus communis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001250Man6P_Isoase-1
IPR011051RmlC_Cupin_sf
IPR014710RmlC-like_jellyroll
IPR016305Mannose-6-P_Isomerase
IPR018050Pmannose_isomerase-type1_CS
Vocabulary: Molecular Function
TermDefinition
GO:0004476mannose-6-phosphate isomerase activity
GO:0008270zinc ion binding
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO:0009298GDP-mannose biosynthetic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006000 fructose metabolic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006013 mannose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016853 isomerase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
WMU16869watermelon unigene v2 vs TrEMBLtranscribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla003612Cla003612.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
WMU16869WMU16869transcribed_cluster


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 26..406
score: 5.4E
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMsTIGR00218TIGR00218coord: 26..432
score: 9.1
IPR011051RmlC-like cupin domainunknownSSF51182RmlC-like cupinscoord: 25..444
score: 2.13E
IPR014710RmlC-like jelly roll foldGENE3DG3DSA:2.60.120.10coord: 288..323
score: 2.0E-59coord: 30..173
score: 2.0E-59coord: 339..444
score: 1.2
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 159..182
score: 1.7E-54coord: 270..285
score: 1.7E-54coord: 65..80
score: 1.7E-54coord: 29..47
score: 1.7E-54coord: 286..305
score: 1.7E-54coord: 324..343
score: 1.7E-54coord: 305..324
score: 1.7
IPR016305Mannose-6-phosphate isomerasePIRPIRSF001480PMIcoord: 23..448
score: 8.8E
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 22..449
score: 2.9E
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 159..167
scor
NoneNo IPR availableunknownCoilCoilcoord: 253..273
scor
NoneNo IPR availableGENE3DG3DSA:1.10.441.10coord: 324..338
score: 2.3E-40coord: 174..281
score: 2.3
NoneNo IPR availablePANTHERPTHR10309:SF0MANNOSE-6-PHOSPHATE ISOMERASEcoord: 22..449
score: 2.9E