Cla003274 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla003274
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Protease inhibitor/seed storage/lipid transfer protein family protein (AHRD V1 **-- D7MKH4_ARALL); contains Interpro domain(s) IPR003612 Plant lipid transfer protein/seed storage/trypsin-alpha amylase inhibitor
Location	Chr9 : 32947858 .. 32948151 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCTCCGTCCTCGTCCTCGTCCTTCTTTTGGACAACGGCGTAACCGCCTATCCACTCTGTAAGATCGACACCACTGAGCTCGGGGTTTGCCGCCCAGCCGTGGCCCCGCCAGAGCATGGCCAGCCTCTACCACTCCCCACGGACGCTTGTTGTTCGGTGGTCCGCCGTGCTGATCTTAAGTGTCTTTGCAATCTCAAATCTGCCCTCCCCTCCATGGGGATTAACCCTGCCAATGCCTTGGCTTTGCCAGCCAAGTGTGGCACCTCATCCCCGCCCGAGTGCCATCTCTAA

mRNA sequence

ATGTTCTCCGTCCTCGTCCTCGTCCTTCTTTTGGACAACGGCGTAACCGCCTATCCACTCTGTAAGATCGACACCACTGAGCTCGGGGTTTGCCGCCCAGCCGTGGCCCCGCCAGAGCATGGCCAGCCTCTACCACTCCCCACGGACGCTTGTTGTTCGGTGGTCCGCCGTGCTGATCTTAAGTGTCTTTGCAATCTCAAATCTGCCCTCCCCTCCATGGGGATTAACCCTGCCAATGCCTTGGCTTTGCCAGCCAAGTGTGGCACCTCATCCCCGCCCGAGTGCCATCTCTAA

Coding sequence (CDS)

ATGTTCTCCGTCCTCGTCCTCGTCCTTCTTTTGGACAACGGCGTAACCGCCTATCCACTCTGTAAGATCGACACCACTGAGCTCGGGGTTTGCCGCCCAGCCGTGGCCCCGCCAGAGCATGGCCAGCCTCTACCACTCCCCACGGACGCTTGTTGTTCGGTGGTCCGCCGTGCTGATCTTAAGTGTCTTTGCAATCTCAAATCTGCCCTCCCCTCCATGGGGATTAACCCTGCCAATGCCTTGGCTTTGCCAGCCAAGTGTGGCACCTCATCCCCGCCCGAGTGCCATCTCTAA

Protein sequence

MFSVLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCLCNLKSALPSMGINPANALALPAKCGTSSPPECHL

Homology

[Close]

BLAST of Cla003274 vs. Swiss-Prot
Match: DIRL1_ARATH (Putative lipid-transfer protein DIR1 OS=Arabidopsis thaliana GN=DIR1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.9e-10
Identity = 34/97 (35.05%), Postives = 52/97 (53.61%), Query Frame = 1


Query: 1   MFSVLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADL 60
           M  ++++ +L+D  V A  LC +   EL  C+PAV+      P    +  CC+ ++ AD 
Sbjct: 11  MAMIVIMAMLVDTSV-AIDLCGMSQDELNECKPAVSKENPTSP----SQPCCTALQHADF 70

Query: 61  KCLCNLKSA--LPSMGINPANALALPAKCGTSSPPEC 96
            CLC  K++  L S G++P  A ALP +CG ++ P C
Sbjct: 71  ACLCGYKNSPWLGSFGVDPELASALPKQCGLANAPTC 102

[Close]

BLAST of Cla003274 vs. TrEMBL
Match: A0A0A0LAS6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G760500 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 8.9e-35
Identity = 70/91 (76.92%), Postives = 79/91 (86.81%), Query Frame = 1


Query: 6   VLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCLCN 65
           +LVLLL   VTAYPLCKIDT++L +CR AV PP+HGQPLPLPT+ CCSVVR ADLKCLCN
Sbjct: 12  LLVLLLPAAVTAYPLCKIDTSDLKLCRSAVTPPDHGQPLPLPTEDCCSVVRHADLKCLCN 71

Query: 66  LKSALPSMGINPANALALPAKCGTSSPPECH 97
           LKS LPSMGI+ ANALALP+KC  +SPPECH
Sbjct: 72  LKSVLPSMGIDTANALALPSKCNVASPPECH 102

[Close]

BLAST of Cla003274 vs. TrEMBL
Match: A0A0A0LBB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G759990 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.4e-24
Identity = 62/92 (67.39%), Postives = 70/92 (76.09%), Query Frame = 1


Query: 4   VLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCL 63
           +L LV++L    TA  LCK+DT EL VCRPAVAP  HG+PL  PT+ CCSV+R ADLKCL
Sbjct: 16  LLFLVMVLAE-TTATLLCKVDTKELCVCRPAVAPL-HGKPLQPPTERCCSVLRSADLKCL 75

Query: 64  CNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           CNLKS LPS GIN   ALALP+KCG  SPPEC
Sbjct: 76  CNLKSILPSAGINATIALALPSKCGIPSPPEC 105

[Close]

BLAST of Cla003274 vs. TrEMBL
Match: K7MTB4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_18G191800 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.1e-19
Identity = 48/92 (52.17%), Postives = 62/92 (67.39%), Query Frame = 1


Query: 4   VLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCL 63
           V  L++ L  G  A  +C ID+++L +CR AV     GQ  P P + CC+V+R+A+L+CL
Sbjct: 14  VAALLIALLGGAQAVAICNIDSSQLNLCRAAVT----GQNPPPPDEKCCAVIRQANLRCL 73

Query: 64  CNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           C+ KS LPS GINP NALALP KCG  SPP C
Sbjct: 74  CSYKSILPSFGINPKNALALPGKCGLQSPPNC 101

[Close]

BLAST of Cla003274 vs. TrEMBL
Match: A0A0B2Q3M5_GLYSO (Putative lipid-transfer protein DIR1 OS=Glycine soja GN=glysoja_037560 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.1e-19
Identity = 48/92 (52.17%), Postives = 62/92 (67.39%), Query Frame = 1


Query: 4   VLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCL 63
           V  L++ L  G  A  +C ID+++L +CR AV     GQ  P P + CC+V+R+A+L+CL
Sbjct: 14  VAALLIALLGGAQAVAICNIDSSQLNLCRAAVT----GQNPPPPDEKCCAVIRQANLRCL 73

Query: 64  CNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           C+ KS LPS GINP NALALP KCG  SPP C
Sbjct: 74  CSYKSILPSFGINPKNALALPGKCGLQSPPNC 101

[Close]

BLAST of Cla003274 vs. TrEMBL
Match: C6T4Z3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.1e-19
Identity = 48/92 (52.17%), Postives = 62/92 (67.39%), Query Frame = 1


Query: 4   VLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCL 63
           V  L++ L  G  A  +C ID+++L +CR AV     GQ  P P + CC+V+R+A+L+CL
Sbjct: 14  VAALLIALLGGAQAVAICNIDSSQLSLCRAAVT----GQNPPPPDEKCCAVIRQANLRCL 73

Query: 64  CNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           C+ KS LPS GINP NALALP KCG  SPP C
Sbjct: 74  CSYKSILPSFGINPKNALALPGKCGLQSPPNC 101

[Close]

BLAST of Cla003274 vs. NCBI nr
Match: gi|449465073|ref|XP_004150253.1| (PREDICTED: putative lipid-transfer protein DIR1 [Cucumis sativus])

HSP 1 Score: 154.1 bits (388), Expect = 1.3e-34
Identity = 70/91 (76.92%), Postives = 79/91 (86.81%), Query Frame = 1


Query: 6   VLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCLCN 65
           +LVLLL   VTAYPLCKIDT++L +CR AV PP+HGQPLPLPT+ CCSVVR ADLKCLCN
Sbjct: 12  LLVLLLPAAVTAYPLCKIDTSDLKLCRSAVTPPDHGQPLPLPTEDCCSVVRHADLKCLCN 71

Query: 66  LKSALPSMGINPANALALPAKCGTSSPPECH 97
           LKS LPSMGI+ ANALALP+KC  +SPPECH
Sbjct: 72  LKSVLPSMGIDTANALALPSKCNVASPPECH 102

[Close]

BLAST of Cla003274 vs. NCBI nr
Match: gi|659093746|ref|XP_008447695.1| (PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo])

HSP 1 Score: 152.9 bits (385), Expect = 2.8e-34
Identity = 71/92 (77.17%), Postives = 80/92 (86.96%), Query Frame = 1


Query: 4   VLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCL 63
           +L+LVLLL   VT+ PLC+IDTT+L VCRPAV PP+HG+ LPLPTDACCSVVRRADLKCL
Sbjct: 10  LLLLVLLLAGAVTSSPLCEIDTTDLEVCRPAVTPPDHGKTLPLPTDACCSVVRRADLKCL 69

Query: 64  CNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           C+LK ALPS GIN ANALALP+KCG S PPEC
Sbjct: 70  CSLKYALPSFGINTANALALPSKCGVSPPPEC 101

[Close]

BLAST of Cla003274 vs. NCBI nr
Match: gi|700203945|gb|KGN59078.1| (hypothetical protein Csa_3G759990 [Cucumis sativus])

HSP 1 Score: 120.2 bits (300), Expect = 2.0e-24
Identity = 62/92 (67.39%), Postives = 70/92 (76.09%), Query Frame = 1


Query: 4   VLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCL 63
           +L LV++L    TA  LCK+DT EL VCRPAVAP  HG+PL  PT+ CCSV+R ADLKCL
Sbjct: 16  LLFLVMVLAE-TTATLLCKVDTKELCVCRPAVAPL-HGKPLQPPTERCCSVLRSADLKCL 75

Query: 64  CNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           CNLKS LPS GIN   ALALP+KCG  SPPEC
Sbjct: 76  CNLKSILPSAGINATIALALPSKCGIPSPPEC 105

[Close]

BLAST of Cla003274 vs. NCBI nr
Match: gi|659093748|ref|XP_008447696.1| (PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo])

HSP 1 Score: 119.0 bits (297), Expect = 4.6e-24
Identity = 59/95 (62.11%), Postives = 71/95 (74.74%), Query Frame = 1


Query: 1   MFSVLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADL 60
           +F  L+ ++++  G +A  LCK+DT +L VCRPAVAP  HG+PL  PT  CCSVVR ADL
Sbjct: 12  LFLRLLFLVMVLAGTSATLLCKVDTKKLCVCRPAVAPL-HGKPLQPPTKRCCSVVRSADL 71

Query: 61  KCLCNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           KCLCNLKS LPS GIN   ALALP+KCG  +PPEC
Sbjct: 72  KCLCNLKSILPSAGINATIALALPSKCGIRTPPEC 105

[Close]

BLAST of Cla003274 vs. NCBI nr
Match: gi|351727813|ref|NP_001235382.1| (uncharacterized protein LOC100527644 precursor [Glycine max])

HSP 1 Score: 102.4 bits (254), Expect = 4.4e-19
Identity = 48/92 (52.17%), Postives = 62/92 (67.39%), Query Frame = 1


Query: 4   VLVLVLLLDNGVTAYPLCKIDTTELGVCRPAVAPPEHGQPLPLPTDACCSVVRRADLKCL 63
           V  L++ L  G  A  +C ID+++L +CR AV     GQ  P P + CC+V+R+A+L+CL
Sbjct: 14  VAALLIALLGGAQAVAICNIDSSQLSLCRAAVT----GQNPPPPDEKCCAVIRQANLRCL 73

Query: 64  CNLKSALPSMGINPANALALPAKCGTSSPPEC 96
           C+ KS LPS GINP NALALP KCG  SPP C
Sbjct: 74  CSYKSILPSFGINPKNALALPGKCGLQSPPNC 101

The following BLAST results are available for this feature:

BLAST of Cla003274 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DIRL1_ARATH	2.9e-10	35.05	Putative lipid-transfer protein DIR1 OS=Arabidopsis thaliana GN=DIR1 PE=1 SV=1	[more]

BLAST of Cla003274 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAS6_CUCSA	8.9e-35	76.92	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G760500 PE=4 SV=1	[more]
A0A0A0LBB0_CUCSA	1.4e-24	67.39	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G759990 PE=4 SV=1	[more]
K7MTB4_SOYBN	3.1e-19	52.17	Uncharacterized protein OS=Glycine max GN=GLYMA_18G191800 PE=4 SV=1	[more]
A0A0B2Q3M5_GLYSO	3.1e-19	52.17	Putative lipid-transfer protein DIR1 OS=Glycine soja GN=glysoja_037560 PE=4 SV=1	[more]
C6T4Z3_SOYBN	3.1e-19	52.17	Putative uncharacterized protein OS=Glycine max PE=2 SV=1	[more]

BLAST of Cla003274 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449465073\|ref\|XP_004150253.1\|	1.3e-34	76.92	PREDICTED: putative lipid-transfer protein DIR1 [Cucumis sativus]	[more]
gi\|659093746\|ref\|XP_008447695.1\|	2.8e-34	77.17	PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo]	[more]
gi\|700203945\|gb\|KGN59078.1\|	2.0e-24	67.39	hypothetical protein Csa_3G759990 [Cucumis sativus]	[more]
gi\|659093748\|ref\|XP_008447696.1\|	4.6e-24	62.11	PREDICTED: putative lipid-transfer protein DIR1 [Cucumis melo]	[more]
gi\|351727813\|ref\|NP_001235382.1\|	4.4e-19	52.17	uncharacterized protein LOC100527644 precursor [Glycine max]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR016140	Bifunc_inhib/LTP/seed_store

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU39021	watermelon unigene v2 vs TrEMBL	transcribed_cluster
WMU41012	watermelon EST collection version 2.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla003274	Cla003274.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU39021	WMU39021	transcribed_cluster
WMU41012	WMU41012	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 46..95 score: 3.
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 21..95 score: 0.
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	unknown	SSF47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 21..95 score: 2.23
None	No IPR available	GENE3D	G3DSA:1.10.110.10		coord: 21..94 score: 5.
None	No IPR available	PANTHER	PTHR33122	FAMILY NOT NAMED	coord: 4..95 score: 5.2
None	No IPR available	PANTHER	PTHR33122:SF4	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN-LIKE PROTEIN	coord: 4..95 score: 5.2

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla003274	Cucurbita maxima (Rimu)	cmawmB009
Cla003274	Cucurbita maxima (Rimu)	cmawmB410
Cla003274	Cucurbita moschata (Rifu)	cmowmB001
Cla003274	Cucurbita moschata (Rifu)	cmowmB404
Cla003274	Wild cucumber (PI 183967)	cpiwmB186
Cla003274	Melon (DHL92) v3.5.1	mewmB212
Cla003274	Watermelon (Charleston Gray)	wcgwmB008
Cla003274	Cucumber (Chinese Long) v2	cuwmB182
Cla003274	Cucumber (Chinese Long) v2	cuwmB563
Cla003274	Cucurbita pepo (Zucchini)	cpewmB531
Cla003274	Cucurbita pepo (Zucchini)	cpewmB753
Cla003274	Bottle gourd (USVL1VR-Ls)	lsiwmB177
Cla003274	Cucumber (Gy14) v2	cgybwmB531
Cla003274	Melon (DHL92) v3.6.1	medwmB197
Cla003274	Silver-seed gourd	carwmB0886
Cla003274	Cucumber (Chinese Long) v3	cucwmB594
Cla003274	Watermelon (97103) v2	wmwmbB042
Cla003274	Wax gourd	wgowmB581
Cla003274	Cucumber (Gy14) v1	cgywmB516