Cla002528 (gene) Watermelon (97103) v1

BLAST of Cla002528 vs. Swiss-Prot
Match: FAB1B_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana GN=FAB1B PE=2 SV=1)

HSP 1 Score: 1672.5 bits (4330), Expect = 0.0e+00
Identity = 940/1838 (51.14%), Postives = 1206/1838 (65.61%), Query Frame = 1

Query: 9    SELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRRCGRVFCSK 68
            SE+VGL+KSW+PWRS+P  VSRDFWMPD+SCRVCYECD QFT INRRHHCR CGRVFC K
Sbjct: 12   SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69   CTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAISLSSS--ST 128
            CTAN IP   +D    R+D E+IRVCNYCF+QW +      DG    S+   LS+S   T
Sbjct: 72   CTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQG-----DGGPHVSNITELSTSPSET 131

Query: 129  SMDSTKSGYTCHSAISNIASTPCS---NGRQYY--DPYCSGAITDEQDNLRNAKTINRTT 188
            S+ S+K+  T +S+   + S P     N R ++  D    G  + E    +  K  +R +
Sbjct: 132  SLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRS 191

Query: 189  SLMTS-----SSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTDEGLNLE 248
            S + +     S       RSDDE D+Y  G Y++D E+ H  ++++YY  +  + G+ ++
Sbjct: 192  SFIATDVEDPSRFALNSIRSDDEYDEY--GAYQTDIETSHSPRANEYYGPMEYN-GMGID 251

Query: 249  -----------RENISTKGSPVNFDTQC----PRGNEESSEDSDENDKGSECDFPSYAMQ 308
                        +  S  GSP+    QC     R   E  +   E+D   EC+  S A  
Sbjct: 252  DVPCKHLGGETADQKSLSGSPLIH--QCLESLIREGSEQFQKKSEHDGRDECEASSPADI 311

Query: 309  NKDN--EPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDACAPGEWGYLHSSLS--SG 368
            + D   EP+DF+NNG+LW+PPEPE+EEDE+  AL  E+D++  A GEWGYL  S S  SG
Sbjct: 312  SDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSG 371

Query: 369  ECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHLSWEAA 428
            E   + +++E+++K  KNVV+GHFRAL+ QLL+ EN+ + DE G++ WL+IIT LSWEAA
Sbjct: 372  EYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAA 431

Query: 429  AVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFV 488
             +LKPD SKSGGMDPGGYVKVKC+A G R +S++VKG++CKKNV +RRM+  I K R  +
Sbjct: 432  NLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLI 491

Query: 489  LGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK 548
            LGG LEYQRV+N LSSFDTLLQQE DHLKMAVAKI A  PN+LLVEKSVSR AQEYLL+K
Sbjct: 492  LGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAK 551

Query: 549  NITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGK 608
            +I+LVLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ + EEH S  GQ GK
Sbjct: 552  DISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGS-TGQVGK 611

Query: 609  KSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGAS 668
            K  KTLM+FE CPKPL  TILLRGA+ DELKKVKHVVQY  FAAYHLALET FLADEGAS
Sbjct: 612  KVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS 671

Query: 669  VPELPLKSPITVALPHNPSNIDRSISAIPGFT------SPSPVKSPEPE-----PIMEIG 728
             PELPL SPITVALP   ++I+RSIS +PGFT      SP+ +   EP+     P+ E+ 
Sbjct: 672  -PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELL 731

Query: 729  QSGKDAVSSRDLSIISHNVVNLNSLEPTLRVKPSATSTSLSFLKQDVSTASRNYMPSSKF 788
             +  +    +D+  I +      S      + P     S  F + ++S    + +  S+ 
Sbjct: 732  STTTNLSIQKDIPPIPY-----GSGWQAREINP-----SFVFSRHNISLNLPDRVIESRN 791

Query: 789  ASEGKRS--LDSKEYSMGMKTIKEEAGEDDDTVSSSGKGEPSSRYTNTIQICRNSLDANT 848
            +    RS  +D+         + +E   +   +S  G    SS+   +I +      +  
Sbjct: 792  SDLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSEL 851

Query: 849  LSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLR 908
              +    ++ P+        T+S +E+FP SPSD Q+ILVSLS+R VWKGT+C+R+HL R
Sbjct: 852  TIAQQQNNEKPKE-------TQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFR 911

Query: 909  IKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLP 968
            IKYYGSFDKPLGRFLRD+LFDQ+YRC SC MPSEAHV+CYTH QGSLTIS KKL D+ LP
Sbjct: 912  IKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLP 971

Query: 969  GEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGC 1028
            GE EGKIWMWHRCL+CPR NGFPPAT RV+MSDAAWGLSFGKFLELSFSN+AAASRVA C
Sbjct: 972  GEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACC 1031

Query: 1029 GHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKEANEVHNRAK 1088
            GH LHRDCLRFYGFG  VACF YA I+V SV++PPS++ F++ N + I +E +EV  RA+
Sbjct: 1032 GHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAE 1091

Query: 1089 TLFAEVCKALPLIPKNESGTEQEDFGVNELDNYIQELEQIINMEKENFEVHFNEVLSGET 1148
             LF+EV  A+  I   E G  +           I ELE+++  EK  FE +  ++L  E 
Sbjct: 1092 LLFSEVLNAISQIA--EKGFRRR----------IGELEEVLQKEKAEFEENMQKILHREV 1151

Query: 1149 TVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDIPTLEEKELDSL 1208
              GQP VDI E+ ++H  +LF SY+W  RL  A+TL K   SD +  +    E+  L   
Sbjct: 1152 NEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKRE--ENEKPPLAKS 1211

Query: 1209 EELVELTLASGRDEFVGGYDNDSLPAESKREIDLGRGGNSGKISR-SKETDREKNI---- 1268
            + L E+   +G +  + G + +  P       D    G++G ++   KE D   ++    
Sbjct: 1212 QTLPEMN--AGTNSLLTGSEVNLNP-------DGDSTGDTGSLNNVQKEADTNSDLYQEK 1271

Query: 1269 DLGGEEDDLFLSPVNSVPLDVGIAIRRANSVGEYPIMTDLSDTLDAVWKG------VIHP 1328
            D GGE       P  S PL+  + +RR  S G+  +M +LS TLDA W G       I  
Sbjct: 1272 DDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPT 1331

Query: 1329 NSVIFEDSFSRTNSAILEPLGG---QSDMEKCTANGVETAHLLHSALVLTKDDSVETITS 1388
            N+ +     + +NS+   P+       D+ +   N  + A+ +  AL     ++ E   S
Sbjct: 1332 NNKVSLPPSTMSNSSTFPPISEGLMPIDLPE-QQNEFKVAYPVSPALPSKNYENSEDSVS 1391

Query: 1389 STSMPSSAYNTKIS---LLNIPKSD-ISEYGPAFISSYRELEKQSHLRLLMHVGSSDTVV 1448
              S+P   +   I+   LL+  K D   E+ P +ISS+RE E Q   RLL+ VG +D VV
Sbjct: 1392 WLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVV 1451

Query: 1449 PVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIKPCDEEGSD 1508
            PVYD+EPTS+IA++L+ PEY  Q S   + + +  +  ++P          +P D+   D
Sbjct: 1452 PVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIP----------RPVDDTIFD 1511

Query: 1509 AYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTITCYFAKRF 1568
              ++  + +E+ILS+  + S    D L +TK LH RV +  +   G VKYT+TCY+AKRF
Sbjct: 1512 PSRSNGSVDESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRF 1571

Query: 1569 EALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFA 1628
            EALR  C PSEL+Y+RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+FA
Sbjct: 1572 EALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFA 1631

Query: 1629 PAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFRHNVTRLYD 1688
            PAYF YLSE+I T  PTCLAK++G+YQV++K LK GKE K+DVL+MENLLF   V RLYD
Sbjct: 1632 PAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYD 1691

Query: 1689 LKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDV 1748
            LKGSSR+RYN D  G NKVLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDT+FLA  DV
Sbjct: 1692 LKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDV 1751

Query: 1749 MDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPTVISPHQYK 1783
            MDYSLLVG+DEEK ELV+GIIDF+RQYTWDKHLE+WVK +GILGGPK+ APTVISP QYK
Sbjct: 1752 MDYSLLVGVDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYK 1785

BLAST of Cla002528 vs. Swiss-Prot
Match: FAB1A_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana GN=FAB1A PE=2 SV=1)

HSP 1 Score: 1643.6 bits (4255), Expect = 0.0e+00
Identity = 922/1826 (50.49%), Postives = 1179/1826 (64.57%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P +S+  N+SRDFWMPD+SC VCYECD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFC+KC AN IP+PS +  +  ++ E+IRVCNYC+KQW +      +G    S   S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSGAITD-------EQDNLRN 180
             S S+ S+ ST    T +S+   I ST   + R   +P  S  ++        EQ N ++
Sbjct: 121  SSPSARSVAST----TSNSSNCTIDSTAGPSPRPKMNPRASRRVSSNMDSEKSEQQNAKS 180

Query: 181  AKTINRTTSLMTSSSSYYGYY-----RSD---DEDDDYYYGMYRSDSESRHISQSDDYYC 240
             ++ +    ++ SS +   ++     RSD   D+DDDY     +S       +Q +DYY 
Sbjct: 181  RRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDFAQS------YAQGNDYYG 240

Query: 241  AVNTDE-------------GLNLERENISTKGSPVNFDTQCPRGNEESSEDSDENDKGSE 300
            A+N DE             G+ +E  NIS  G P + D               EN     
Sbjct: 241  AINLDEVDHIYGSHEAHDVGVKIE-PNIS--GFPPDQDLDSLNTETIDKTRQQENGWNDV 300

Query: 301  CDFPSYAMQNKDNEPMDFQNNGILWLPPEPEDEEDEK-ALLFEDDDDACAPGEWGYLHSS 360
             +      ++ + E +DF+++G+LWLPPEPE+EEDE+ A+L +DD D    G+WGYL  S
Sbjct: 301  KEGSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPS 360

Query: 361  LSSGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHLS 420
             S  E     K         KNVVEGHFRALV QLLE +NLP+ +E  E+GWLDIIT LS
Sbjct: 361  NSFNEKDFHSKDKSS--GAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420

Query: 421  WEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKP 480
            WEAA +LKPDTSKSGGMDPGGYVKVKC+  G+RSES++VKG++CKKNVAHRRMT  I KP
Sbjct: 421  WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            R  +LGGALEYQR++N LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQEY
Sbjct: 481  RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540

Query: 541  LLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDG 600
            LL+K+I+LVLNIKRSLLERI+ CTGA I+P++D + S KLGYCD F VE FVE H S   
Sbjct: 541  LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS-PC 600

Query: 601  QGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            Q  KK  KTLMFF+GCPKPL CTILL+GA  DELKKVKHV+QY  FAAYHLALET FLAD
Sbjct: 601  QVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSGKDAV 720
            EGAS+ ELPL++PITVALP  PS ++RSIS IPGFT  S  KSP  E   E  ++  D  
Sbjct: 661  EGASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLT 720

Query: 721  SSRDLSIISHNVVNLNSLEPTLRVKPSATSTSLSFLKQDVSTASRNYMPSSKFASEG-KR 780
                 +  S        L+   R+ PS        L  ++ T       +     +G   
Sbjct: 721  G----NFTSSKTHFQGKLDGNDRIDPSER------LLHNLDTVYCKPPETITSKDDGLVP 780

Query: 781  SLDSKEYSMGMKTIKEEAGEDDDTVSSSGKGEPSSRYTNTIQICRNSLDANTLSSNDSTS 840
            +L+S++ S     ++E + + D     SG  E         Q+           +NDS  
Sbjct: 781  TLESRQLSF---HVEEPSVQKDQWSVLSGATE---------QVTDGGY------TNDSAV 840

Query: 841  QGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFD 900
             G ++ N      +SS+ DF  S SD Q+ILVSLSTRCVWKG++C+RAHLLRIKYYGSFD
Sbjct: 841  IGNQNFNRQ-EQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFD 900

Query: 901  KPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIW 960
            KPLGRFLRDNLFDQ+  C SC MP+EAH++CYTH QGSLTIS KKLP+  LPG+ EGKIW
Sbjct: 901  KPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKIW 960

Query: 961  MWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDC 1020
            MWHRCLKCPR NGFPPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDC
Sbjct: 961  MWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDC 1020

Query: 1021 LRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKEANEVHNRAKTLFAEVCK 1080
            LRFYGFGR VACF YA+IN+ +V +PP+ + F++ N E + KE+ EV  +A+ LF EV +
Sbjct: 1021 LRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQE 1080

Query: 1081 ALPLIPKNESGTEQEDFGVNELDNYIQELEQIINMEKENFEVHFNEVLSGETTVGQPTVD 1140
            AL  I     G   +    N++   ++EL  ++   K+ ++    ++L+     GQPT+D
Sbjct: 1081 ALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLN-VVKDGQPTID 1140

Query: 1141 IFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDIPTLEEKELDSLEELVELTL 1200
            I  INKL   ++F SY W + L  AA + + ++ ++  N  P +  + + SLE+L +  +
Sbjct: 1141 ILLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKV 1200

Query: 1201 ASGRDEFVGGYDNDSLPAESKREIDLGRG----GNSGKISRSKETDREKNIDLGGEEDDL 1260
             S          NDSL  ++  E  L +G      SGK +  ++   ++  D   E D  
Sbjct: 1201 KSIPTHV--AICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPS 1260

Query: 1261 FLSPVNSVPLDVGIAIRRANSVGEYPIMTDLSDTLDAVWKGVIHPNSVIFEDSFSRTNSA 1320
                 N V  +    ++ A++  ++   TDLSDTLDA W G             + + + 
Sbjct: 1261 EGGKDNFV--ESSQVVKPAHTESQFQ-ATDLSDTLDAAWIG-----------EQTTSENG 1320

Query: 1321 ILEPLGGQSDMEKCTANGVETAHL----LHSALVLTKDDSVETITSSTSMPSSAYNTKIS 1380
            I  P          + NG +   L      S L      + +  T+   +PS ++   ++
Sbjct: 1321 IFRP-----PSRAASTNGTQIPDLRLLGSESELNFKGGPTNDEHTTQVQLPSPSFYYSLN 1380

Query: 1381 ---LLNIPKSDISEYGPAFISSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAFSL 1440
                LN  K  ++E  P ++SSYRELE +S  RLL+ +G +D V+PVYD+EPTSIIA++L
Sbjct: 1381 KNYSLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYAL 1440

Query: 1441 LMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIKPCDEEGSDAYKTLRTNEETILSM 1500
               EY AQMS  +K  +  D+  S  +  SV + S+    +   D  ++L + +E +  +
Sbjct: 1441 TSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQL 1500

Query: 1501 PRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTITCYFAKRFEALRKKCCPSELDYV 1560
              +         L+ KDLH R+ F +E P G VKY++TCY+AK FEALR  CCPSE D++
Sbjct: 1501 LHSS--------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFI 1560

Query: 1561 RSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPAYFIYLSEAIRTGC 1620
            RSL RC++WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESFI+F PAYF YL+E+I T  
Sbjct: 1561 RSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKS 1620

Query: 1621 PTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNSDPCG 1680
            PT LAK++G+YQVSSKHLKGGKEFK+DVLVMENLLF+ N TRLYDLKGS+R+RYN D  G
Sbjct: 1621 PTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSG 1680

Query: 1681 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1740
             N VLLDQNL+E+MPTSPIF+GSKAKRLLERAVWNDTSFLASI VMDYSLLVG+DEE+ E
Sbjct: 1681 SNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNE 1740

Query: 1741 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPTVISPHQYKKRFRKAMTAYFLMVP 1786
            LV+GIIDFMRQYTWDKHLETWVK SG+LGGPK++ PTVISP QYKKRFRKAMTAYFLMVP
Sbjct: 1741 LVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFLMVP 1749

BLAST of Cla002528 vs. Swiss-Prot
Match: FAB1C_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana GN=FAB1C PE=2 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.5e-194
Identity = 366/865 (42.31%), Postives = 524/865 (60.58%), Query Frame = 1

Query: 281  QNKDNEPMDFQNNGILWLPPEPEDEEDEKA---LLFEDDDDACAPGEWGYLHSSLSSGEC 340
            ++K  +P+DF+NNG +W PP PEDE D+       ++D+DD        +  SS  S   
Sbjct: 248  EDKLQQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHI 307

Query: 341  PMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHLSWEAAAV 400
            P K K  E+  +  + VV  HFRALV +LL  E L   D+     WLDI+T L+W+AA  
Sbjct: 308  PTKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANF 367

Query: 401  LKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLG 460
            +KPDT   G MDPG YVK+KCVA G ++ESI+++GI+C KN+ H+RM      PR  +L 
Sbjct: 368  VKPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLA 427

Query: 461  GALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNI 520
            G+LEYQRV   L+SF+TLLQQE +H+K  +AKI++  PNVLLVEKS S +AQ+YLL K I
Sbjct: 428  GSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEI 487

Query: 521  TLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKS 580
            +LVLN+KRSLL+RIA CTGA + P++D +++ +LG+C+ FR E  +E+H++G+ Q  +K 
Sbjct: 488  SLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGN-QSNRKP 547

Query: 581  TKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVP 640
            ++TLM+FEGCP+ L CT++LRG+  +ELKKVKHV+QYA FAAYHL+LET FLADEGAS+P
Sbjct: 548  SRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLP 607

Query: 641  ELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSGKDAVSSRDLS 700
            ++ LK P  V        ID  IS I    + SP ++ + + ++E      +  +     
Sbjct: 608  KIRLKQPGMVRTASQRRIIDEGISLI----TQSPTET-DSQALLETAAHEDEHTAPMPEH 667

Query: 701  IISHNVVNLNSLEPTLRVKPSATSTSLSFLKQDVSTASRNYMPSSKFASEGKRSLDSKEY 760
             +  ++      +PT    PS+   +       ++    N + +  ++S     L     
Sbjct: 668  EVCESL--CEDFDPTQIFPPSSEVETEQ--SDTLNGDFANNLVTRSYSSNQLNDLHEPTL 727

Query: 761  SMGMKTIKEEAGEDDDTVSSSGKGEPSSRYTNTIQICRNSLDANTLSSNDSTSQGPESSN 820
             +  + I E   +       +G+GE  ++  N             L  ++S  +   SS 
Sbjct: 728  CLSSE-IPETPTQQPSGEEDNGRGEEENQLVN----------PQDLPQHESFYEDDVSS- 787

Query: 821  ANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFL 880
                       ++  +    Q+ILVS S+RCV K ++C+R+ LLRIK+YGSFDKPLGR+L
Sbjct: 788  -----------EYFSAADSHQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYL 847

Query: 881  RDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLK 940
            +D+LFD+   C SC    +AHV CY+H  G+LTI+ ++LP   LPGE +GKIWMWHRCL+
Sbjct: 848  KDDLFDKTSSCRSCKELVDAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLR 907

Query: 941  CPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFG 1000
            C   +G PPATRRV+MSDAAWGLSFGKFLELSFSN+A A+RVA CGH L RDCLRFYGFG
Sbjct: 908  CAHVDGVPPATRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFG 967

Query: 1001 RTVACFHYAAINVQSVHIPPSMIEFH-HNNLERINKEANEVHNRAKTLFAEVCKALPLIP 1060
              VA F Y+ IN+ +V +PPSM+EF+ H   E I  EA E+  + +T++ E+   L  + 
Sbjct: 968  NMVAFFRYSPINILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRME 1027

Query: 1061 KNESGTEQEDFGVNELDNYIQELEQIINMEKENFEVHFNEVLSGETTVGQPTVDIFEINK 1120
            +  S  E E     +L + I  L   +  EK+ ++     +      + Q ++DI E+N+
Sbjct: 1028 EKSSLLEPEQSEACDLHSRIIGLIDQLVKEKDEYDDALQPIFEENLQI-QGSLDILELNR 1078

Query: 1121 LHMHVLFLSYIWVQRLNYAATLRKK 1142
            L   ++  ++ W  +L    +  KK
Sbjct: 1088 LRRALMIGAHAWDHQLYLLNSQLKK 1078

HSP 2 Score: 448.7 bits (1153), Expect = 2.9e-124
Identity = 245/525 (46.67%), Postives = 333/525 (63.43%), Query Frame = 1

Query: 1255 TDLSDTLDAVWKGVIH----PNSVIFEDSFSRTNSAI---LEPLGGQS-----DMEKCTA 1314
            T LS+ +D+ W G         ++   + FS  NS++     P+  QS       ++   
Sbjct: 1137 TSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQSFDSAIRFQERIQ 1196

Query: 1315 NGVETAHLLHSAL--VLTKDDSVETITSSTSMPSSAYNTKISLLNIPKSD-ISEYGPAFI 1374
             G+  + L  S L       +    +    S     Y+  + L  + K D I    P +I
Sbjct: 1197 KGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPL-EVQKLDLIVGSAPTYI 1256

Query: 1375 SSYRELEKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASD 1434
            SS  ++   + + L+   G +D VVPVYD++P S++++++   EY   +    K + +S 
Sbjct: 1257 SSASQMADGARM-LIPQRGLNDIVVPVYDDDPASVVSYAINSKEYKEWIVN--KGLASSS 1316

Query: 1435 TARSLPIIGSVKMNSIKPCDEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHT 1494
            ++ +L         + +  +      +++L  + + I       S         T     
Sbjct: 1317 SSSNL---------NNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKSPHLTISFSD 1376

Query: 1495 RVCFVNETPSGPVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFF 1554
            R    +    G VK+++TCYFA +F+ LRK CCPSE+D+VRSLSRC+RW AQGGKSNV+F
Sbjct: 1377 RASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYF 1436

Query: 1555 AKTLDERFIIKQVTKIELESFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKG 1614
            AK+LDERFIIKQV K EL+SF  FAP YF YL E++ +G PTCLAK++G+YQVS KH KG
Sbjct: 1437 AKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKG 1496

Query: 1615 GKEFKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIF 1674
            GKE K+D++VMENL +   ++R+YDLKGS+RSRYN +  G +KVLLD NL+E++ T PIF
Sbjct: 1497 GKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLETLRTEPIF 1556

Query: 1675 LGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLET 1734
            LGSKAKR LERA+WNDT+FLAS+DVMDYSLLVG DEE++ELV+GIIDFMRQYTWDKHLET
Sbjct: 1557 LGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQYTWDKHLET 1616

Query: 1735 WVKNSGILGGPKDAAPTVISPHQYKKRFRKAMTAYFLMVPDQWPS 1765
            WVK SGILGGPK+A+PT++SP QYK+RFRKAMT YFL VP+ W S
Sbjct: 1617 WVKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPWTS 1648

BLAST of Cla002528 vs. Swiss-Prot
Match: FAB1D_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana GN=FAB1D PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 1.7e-108
Identity = 302/937 (32.23%), Postives = 447/937 (47.71%), Query Frame = 1

Query: 211  DSESRHISQSDDYYCAVNTDEGLNLERENISTKGSPVNFDTQCPRGNEESSEDSDENDKG 270
            D  S H SQ D     V   + + ++R    +K  P +        +E+S E+S EN   
Sbjct: 31   DECSSHSSQED-----VELTKEVKVDRLERKSKSMPSDILDIL---DEKSKENSVEN--- 90

Query: 271  SECDFPSYAMQNKDNEPMDFQNNGILWLPPEPEDEEDEKALLFEDDDDACAPGEWGYLHS 330
                F S    + D+ P+        W PPEPE+ EDE   +F DDDD C  G      S
Sbjct: 91   --VQFLSDREDDSDDVPV--------WEPPEPENPEDEVDGVFADDDDDCCDGSKWNKAS 150

Query: 331  SLS--SGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIIT 390
             L   S E   K K  E+ R+      +  F+ +V QL+++    I +E G   W +I+ 
Sbjct: 151  LLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSI-EESGY--WFEIVA 210

Query: 391  HLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGI 450
             L WEAA++LKP       +DP  Y+KVKC+A G   +S + KG++ KK+ A + M    
Sbjct: 211  RLCWEAASMLKPAIDGKS-VDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATKY 270

Query: 451  SKPRFFVLGGALEYQRVTNHLSSFDTLLQQE---LDHLKMAVAKIDAHHPNVLLVEKSVS 510
              PR  ++ G L +    +  SS  ++ Q     L ++K  V  I+A  P+V+LVEKSVS
Sbjct: 271  EHPRIMLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSVS 330

Query: 511  RHAQEYLLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEE 570
            R  Q+ +L K +TLV ++K   L+RI+ C G+ I+ +VD ++S KL +CD+FR+E  VEE
Sbjct: 331  RDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVEE 390

Query: 571  HDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALE 630
            H++  G+  KK TKTLMF EGCP  L CTILL+G   + LKKVK VVQY+   AYHL LE
Sbjct: 391  HNAA-GESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVVQYSFILAYHLMLE 450

Query: 631  TCFLADEGASVPELPLKSPITVALPHNPSNIDRSISAIPGFT-SPSPVKSPEPEPIMEIG 690
              FLAD       +  K   +  +             I  F+ SPSP +SP     + + 
Sbjct: 451  ASFLADRHTMFSTIFAKEATSCVVE------------IENFSPSPSPRESPSEAVDIPVS 510

Query: 691  QSGKDAVSSRDLSIISHNVVNLNSLEPTLRVKPSATSTSLSFLKQDVSTASRNYMPSSKF 750
             +G D                    E T+++   A    +   + D      ++ P +  
Sbjct: 511  -NGFD--------------------EQTIQINGEADGEKVGTWESDGDHVF-SHEPYNPV 570

Query: 751  ASEGKRSLDSK-EYSMGMKTIKEEAGEDDDTVSSSGKGEPSSRYTNTIQICRNSLDANTL 810
               G  SL ++    +G     E      DT  S+         T+ +   R S + +T 
Sbjct: 571  IFTGFSSLSARLSKYLGFVQNPESVPVSVDTDVST---------TSNLDSIRES-EEDTA 630

Query: 811  SSNDSTS------QGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDR 870
              N+         + P +S+++    KS  E+   S  + Q+ILV +S R   +G +CD+
Sbjct: 631  EKNEDKQPLLLDPELPVNSSSDDGDNKSQTENDIESTLESQSILVLVSKRNALRGIMCDQ 690

Query: 871  AHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISAKKLP 930
             H   IK+Y  FD PL +FLRD +F+Q   C +C    EAH+  Y H    LTI  K++P
Sbjct: 691  RHFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIP 750

Query: 931  DFF-LPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAA 990
                L GE +GKIWMW RC KC   N    +T+RV++S AA  LSFGKFLELSFS     
Sbjct: 751  VAKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFL 810

Query: 991  SRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFH-HNNLERINKEAN 1050
            +R + CGH    D L F+G G  VA   Y+ +   +V +PP  +E         + KE  
Sbjct: 811  NRSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQ 870

Query: 1051 EVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYIQELEQIINMEKENFEVHFN 1110
             V  +  +LF +   A   + +  S     D         +  +E+++  E+  FE +  
Sbjct: 871  TVFTKGISLFED---AAGFLKRLRSQFTNSDLRYQRARKLLSNIEELLKHERCIFEENIK 890

Query: 1111 EVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRL 1133
                   T+   +  +  +N++   +L  + IW  RL
Sbjct: 931  NSFDKAKTIDDVSHRLLRLNRMRWELLLQALIWNYRL 890

HSP 2 Score: 317.0 bits (811), Expect = 1.3e-84
Identity = 185/399 (46.37%), Postives = 250/399 (62.66%), Query Frame = 1

Query: 1366 EKQSHLRLLMHVGSSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLP 1425
            E+ S LR+ +     D +V  Y++E +S+IA +L      A ++  E     S    S  
Sbjct: 1080 EESSRLRISLR--DDDFIVSDYEDELSSLIACAL------AHLNNEE-----SKKPLSRC 1139

Query: 1426 IIGSVKMNSIKPCDEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVN 1485
            I GS++       D + +D   +  ++E T     R E+L   ++L         V F +
Sbjct: 1140 IHGSLQGFLDNNQDSKQTDRDVSRFSSEST----NRLETLPPPEVL---------VTFGS 1199

Query: 1486 ETPSGPVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDE 1545
                G  KY+I   +A  F  LRK+CC SELDY+ SLSRCK W A+GGKS   FAKTLD+
Sbjct: 1200 VKSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDD 1259

Query: 1546 RFIIKQVTKIELESFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLK-GGKEFK 1605
            RFI+K++ K E ESF+ FA  YF Y+ ++   G  TCLAKV+G++QV+ +  K GGKE +
Sbjct: 1260 RFIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIR 1319

Query: 1606 VDVLVMENLLFRHNVTRLYDLKGSSRSRYNSDPC-GKNKVLLDQNLIESMPTSPIFLGSK 1665
             D++VMENL F   VTR YDLKG+  +R+ +    G++ VLLDQN +  M  SP+++   
Sbjct: 1320 HDLMVMENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKT 1379

Query: 1666 AKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKN 1725
            +K+ L+RAV+NDTSFL SI+VMDYSLLVG+D+E  ELV GIID++RQYTWDK LETWVK+
Sbjct: 1380 SKQNLQRAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKS 1439

Query: 1726 SGILGGPKDAAPTVISPHQYKKRFRKAMTAYFLMVPDQW 1763
            S ++  PK+  PTVISP  YK RFRK M  +FL VPDQW
Sbjct: 1440 SLVV--PKNVQPTVISPIDYKTRFRKFMKTHFLCVPDQW 1450

BLAST of Cla002528 vs. Swiss-Prot
Match: FAB1_YEAST (1-phosphatidylinositol 3-phosphate 5-kinase FAB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FAB1 PE=1 SV=3)

HSP 1 Score: 282.0 bits (720), Expect = 4.7e-74
Identity = 169/421 (40.14%), Postives = 243/421 (57.72%), Query Frame = 1

Query: 1380 SDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPE-KVMEASDTARSLPI-----------I 1439
            +D+ V + ++EP+S+IAF L   +Y  +M     +  +   TA + P             
Sbjct: 1857 TDSDVIIREDEPSSLIAFCLSTSDYRNKMMNLNVQQQQQQQTAEAAPAKTGGNSGGTTQT 1916

Query: 1440 GSVKMNSIKPC-----DEEGSDA-YKTLRTNEETILSMP-------------RTESLQFE 1499
            G   +N I P        +G D+   +L T +E +L+ P             +T S    
Sbjct: 1917 GDPSVN-ISPSVSTTSHNKGRDSEISSLVTTKEGLLNTPPIEGARDRTPQESQTHSQANL 1976

Query: 1500 DLL-----LFTKDLHTRVCFVNETPSGPVKYTITCYFAKRFEALRKKCCPSELDYVRSLS 1559
            D L     + TK   T + +  E   G    +   +F + F+  RK C   E ++++SLS
Sbjct: 1977 DTLQELEKIMTKKTATHLRYQFE--EGLTVMSCKIFFTEHFDVFRKICDCQE-NFIQSLS 2036

Query: 1560 RCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPAYFIYLSEAIRTGCPTCL 1619
            RC +W + GGKS   F KTLD+RFIIK+++  ELE+FI+FAP+YF Y+++A+    PT L
Sbjct: 2037 RCVKWDSNGGKSGSGFLKTLDDRFIIKELSHAELEAFIKFAPSYFEYMAQAMFHDLPTTL 2096

Query: 1620 AKVVGMYQVSSKH-LKGGKEFKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNSDPCGKNK 1679
            AKV G YQ+  K  +   K +K+DV++MENL +    TR++DLKGS R+R+       N+
Sbjct: 2097 AKVFGFYQIQVKSSISSSKSYKMDVIIMENLFYEKKTTRIFDLKGSMRNRHVEQTGKANE 2156

Query: 1680 VLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVV 1739
            VLLD+N++E +  SPI +    K+LL  +VWNDT FLA ++VMDYSL++G+D E   L V
Sbjct: 2157 VLLDENMVEYIYESPIHVREYDKKLLRASVWNDTLFLAKMNVMDYSLVIGIDNEGYTLTV 2216

Query: 1740 GIIDFMRQYTWDKHLETWVKNSGILGGPK-DAAPTVISPHQYKKRFRKAMTAYFLMVPDQ 1763
            GIIDF+R +TWDK LE+WVK  G++GG      PTV++P QYKKRFR+AM  Y LMVPD 
Sbjct: 2217 GIIDFIRTFTWDKKLESWVKEKGLVGGASVIKQPTVVTPRQYKKRFREAMERYILMVPDP 2273

HSP 2 Score: 133.3 bits (334), Expect = 2.7e-29
Identity = 74/220 (33.64%), Postives = 123/220 (55.91%), Query Frame = 1

Query: 413  YVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSF 472
            YVK+K ++GG    S  + G++  K +  + M R +  PR  ++   LEYQ+  NH  S 
Sbjct: 845  YVKIKRISGGSPQNSEYIDGVVFSKALPSKTMPRHLKNPRILLIMFPLEYQKNNNHFLSI 904

Query: 473  DTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIA 532
            +++ +QE ++L   V+++ + HP+++ V  +VS +A E L    I +  N+K  ++ERIA
Sbjct: 905  ESVFRQEREYLDKLVSRLKSLHPDIIYVGANVSGYALELLNDSGIVVQFNMKPQVIERIA 964

Query: 533  SCTGANIIPTVDHVA-SLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPL 592
              T A+I  +VD +A ++K+G C+ F V++++  + S          KT  F  GC   L
Sbjct: 965  KLTEADIAISVDKLATNIKMGECETFEVKSYIYGNIS----------KTYTFLRGCNPEL 1024

Query: 593  CCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 632
              TILLRG S + L+K+K V ++  +A + L LE+ F  D
Sbjct: 1025 GGTILLRGDSLENLRKIKQVSEFMVYAIFSLKLESSFFND 1054

HSP 3 Score: 70.9 bits (172), Expect = 1.6e-10
Identity = 45/161 (27.95%), Postives = 68/161 (42.24%), Query Frame = 1

Query: 28  VSRDFWMPDKSCRVCYECDSQFTFINRRHHCRRCGRVFCSKCTANFIPAPSTDPTNVRDD 87
           +S+++WM D+S + C+ C   F    R+HHCR CG++FCS CT         D       
Sbjct: 232 LSKEYWMKDESSKECFSCGKTFNTFRRKHHCRICGQIFCSSCTLLI----DGDRFGCH-- 291

Query: 88  SEKIRVCNYCFKQWGKEPSALQDGDKATSSAISLSSSSTSMDSTKSGYTCHSAISNIAST 147
             K+RVC  C++       +  + + +T       S S S  S  + Y+   +  ++ S 
Sbjct: 292 -AKMRVCYNCYEHADTYEDSSDEENDSTMQLNEPRSRSRSRSSNTNPYSHSHSHLHLISQ 351

Query: 148 PCSNGRQYYDPYCSGAITDEQDNLRNAKTIN--RTTSLMTS 187
              NG   +DP    A TD          +N     S+MTS
Sbjct: 352 DNHNGTDLHDPV---AATDNPQQQNEVYLLNDDDVQSIMTS 382

BLAST of Cla002528 vs. TrEMBL
Match: A0A0A0M3A5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G662800 PE=4 SV=1)

HSP 1 Score: 3313.9 bits (8591), Expect = 0.0e+00
Identity = 1637/1790 (91.45%), Postives = 1690/1790 (94.41%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MGHPENKIS+LVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFCS+CTANFIPAPSTD TN R+DSEKIRVCNYCFKQWGKEPS+LQDGDKATSSA+S
Sbjct: 61   CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSGAITDEQDNLRNAKTINRT 180
            LSSSSTSM STKSGYTCHSAIS+IASTPCS GRQYYDPYCS A+ DE DNLRN KTINRT
Sbjct: 121  LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTDEGLNLERENI 240
            TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN DEG NLEREN 
Sbjct: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPSYAMQNKDNEPMDFQNNGILWLPP 300
            STKGSPVNFDTQ PRGNEESSEDSDENDKGSECDFPSYAMQNK++EPMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFEDDDDACAPGEWGYLHSSLSSGECPMKGKSSEDYRKTAKNVVEGHFR 360
            EPEDEEDEKALLFEDDDD CAPGEWGYLHSSLSSGECPMKGKSSEDY+KTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVCQLLEAENLPIGDEPGEDGWLDIITHLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
            ALV QLLEAENLPIGD P EDGWLDIIT+LSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIASCTGANII 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIA CTGANII
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPL SPITVALPHNPSN+DRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNVDRSI 660

Query: 661  SAIPGFTSPSPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVVNLNSLEPTLR-VKPSA 720
            SAIPGFT P PVKSPEPEPIMEIGQSGKDAVSSRD SIISHNVVNLNSLEPT   ++ +A
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSSLLESNA 720

Query: 721  TSTSLSFLKQDVSTASRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVSSSG 780
            TS S SFLKQD ST     M SS F SEGKRSL SKEYSMGM  IKEEAGEDDD VSSSG
Sbjct: 721  TSPSFSFLKQDFST-----MISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDPVSSSG 780

Query: 781  KGEPSSRYTNT-IQICRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQ 840
            K E SSR+ NT + + RNSLDANT S N+STSQGPE+ N ++VTTKSSREDFPLSPSDQQ
Sbjct: 781  KREASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPLSPSDQQ 840

Query: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900
            NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH
Sbjct: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900

Query: 901  VNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960
            V+CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW
Sbjct: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960

Query: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020
            GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS
Sbjct: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020

Query: 1021 MIEFHHNNLERINKEANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYIQE 1080
            MIEFH NNLE INKEANEVHNRAKTLF EVCKALPLIPKNESG EQEDFGV+EL+NYIQE
Sbjct: 1021 MIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDELNNYIQE 1080

Query: 1081 LEQIINMEKENFEVHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATL 1140
            LEQIINMEKENFEVHFN++LSG TTV QPTVDIFEINKLHMH+LFLSYIWVQRLNY ATL
Sbjct: 1081 LEQIINMEKENFEVHFNKLLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATL 1140

Query: 1141 RKKHFSDSSSNDIPTLEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGR 1200
            RKKH  D SSNDI  L EKEL+SLEELVELTLASGRDEF GG DNDSLPAESKRE  LGR
Sbjct: 1141 RKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPAESKREAGLGR 1200

Query: 1201 GGNSGKISRSKETDREKNIDLGGEEDDLFLSPVNSVPLDVGIAIRRANSVGEYPIMTDLS 1260
             GNSG ISR  +TDREKNIDLGG+EDD  LSPVN  P+DVGI IRRANSVGEYPIMT + 
Sbjct: 1201 EGNSGNISRLNKTDREKNIDLGGQEDDFCLSPVNLDPVDVGIGIRRANSVGEYPIMT-VP 1260

Query: 1261 DTLDAVWKGVIHPNSVIFEDSFSRTNSAILEPLGGQSDMEKCTANGVETAHLLHSALVLT 1320
             TLDAVWKGVIHPNS+IF+DSFSRT SAILEPL GQSDMEKCTAN +ETAHLLHSALVLT
Sbjct: 1261 GTLDAVWKGVIHPNSLIFDDSFSRTTSAILEPLAGQSDMEKCTANRIETAHLLHSALVLT 1320

Query: 1321 KDDSVETITSSTSMPSSAYNTKISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMHVG 1380
            +DDSVETI +STSMP SAYNTKISLLNIPKSDIS+Y   FISSYRELEKQSHLRLLMHVG
Sbjct: 1321 RDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIKPC 1440
            SSDTVVPVYDEEPTSIIA+S+LMPEYIAQMSEP K MEASDTARSLP+IGSVKMNSIK C
Sbjct: 1381 SSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSLPVIGSVKMNSIKTC 1440

Query: 1441 DEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTITC 1500
            DEEGSDAYKTLR+NEETILSMPRTESLQFEDL+  +KDLHTRVCF+NETP GPVKYTITC
Sbjct: 1441 DEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFINETPLGPVKYTITC 1500

Query: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
            YFAKRFEALRKKCCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIELE
Sbjct: 1501 YFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFRHN 1620
            SFIQF+ AYFIYLS+AI TGCPTCLAKVVGMYQVSSKHLKGGKE KVDVLVMENLLFRHN
Sbjct: 1561 SFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRHN 1620

Query: 1621 VTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680
            VTRLYDLKGSSRSRYN D  GKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNPDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680

Query: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPTVI 1740
            LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPK+AAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTAYFLMVPDQWPSTTVSPSESHPDLCDDNSEPENSWD 1789
            SPHQYKKRFRKAMT YFLMVPDQWPS+TVSPSESHPDLCD+NSEPENSWD
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWD 1784

BLAST of Cla002528 vs. TrEMBL
Match: A0A061GSN9_THECC (Phosphatidylinositol-4-phosphate 5-kinase family protein, putative isoform 1 OS=Theobroma cacao GN=TCM_037451 PE=4 SV=1)

HSP 1 Score: 2006.1 bits (5196), Expect = 0.0e+00
Identity = 1063/1850 (57.46%), Postives = 1325/1850 (71.62%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MG+P+NK+S+LV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1    MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFC+KCTAN +PAPS      ++DSE+IRVCNYCFKQW +  +A+  G  A S  +S
Sbjct: 61   CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSG----------AITDEQDN 180
             S S+TS+ STKS  TC+S+ S + STP S G  +   Y SG          A   EQ+N
Sbjct: 121  PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181  LRNAKTINRTTSLMTSSSSYYGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
              +  + N +++ + SSS+++G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181  KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241  TD-------------EGLNLERENISTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDF 300
                           +G N++ +++S    P NF+ Q   G ++  E+ +E +   E + 
Sbjct: 241  IGSIDRVYGSDKVHPDGGNMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGEV 300

Query: 301  PSYAMQNKDNEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDACAPGEWGYLHSSLS 360
            P+Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWGYL SS S
Sbjct: 301  PAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSNS 360

Query: 361  --SGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHLS 420
              SGE   + KS+E++R+  KNVVEGHFRALV QLL+ ENLP+GDE G D WLDIIT+LS
Sbjct: 361  FGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYLS 420

Query: 421  WEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKP 480
            WEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I KP
Sbjct: 421  WEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            RF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY
Sbjct: 481  RFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540

Query: 541  LLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDG 600
            LL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  G
Sbjct: 541  LLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA-G 600

Query: 601  QGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            QGGKK TKTLMFF+GCPKPL  TILL+GA+GDELKKVKHVVQY  FAAYHLALET FLAD
Sbjct: 601  QGGKKLTKTLMFFDGCPKPLGYTILLKGANGDELKKVKHVVQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSGKDAV 720
            EGA++PELPLKSPITVALP  P++IDRSIS IPGFT PS  K    +PI E+ +S K  +
Sbjct: 661  EGATLPELPLKSPITVALPDKPASIDRSISTIPGFTVPSSGKPMASQPINELQKSNKVVI 720

Query: 721  SSR---------------DLSIISHNVVNLNSLEP--TLRVKPSATSTSLSFLKQDVSTA 780
            S R                 S +S  +    +L+   +  ++   +  SLS L++++S  
Sbjct: 721  SDRPSSANVEPPCESRGASSSCLSKGLHTQTTLKEYASSSIEAITSLNSLSALRENIS-- 780

Query: 781  SRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVS-SSGKGEPSSRYTNTIQI 840
            S   + S   A      +D KE S+  KT   EA  DD  +S      E   +   +   
Sbjct: 781  SHGNVLSLNHAFSKVNGIDPKE-SVQTKTASSEAVMDDGFISICQSLLEAPDQGGGSNHT 840

Query: 841  CRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGT 900
              N L AN L      S   ++SN N     SS+E+FP SPSD Q+ILVSLSTRCVWKGT
Sbjct: 841  DGNMLVANHLGVPQLASSKRDTSNNNE-EVGSSKEEFPPSPSDHQSILVSLSTRCVWKGT 900

Query: 901  ICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISA 960
            +C+R+HL RIKYYG+FDKPLGRFLRD+LFDQ++RC SC MPSEAHV+CYTH QGSLTIS 
Sbjct: 901  VCERSHLFRIKYYGNFDKPLGRFLRDHLFDQSFRCRSCEMPSEAHVHCYTHRQGSLTISV 960

Query: 961  KKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNN 1020
            +KLP+  LPG+ EGKIWMWHRCL+CPR N FPPATRR++MSDAAWGLSFGKFLELSFSN+
Sbjct: 961  RKLPELPLPGQREGKIWMWHRCLRCPRANKFPPATRRIVMSDAAWGLSFGKFLELSFSNH 1020

Query: 1021 AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKE 1080
            AAASRVA CGH LHRDCLRFYGFGR VACF YAAI+V SV++PP  +EF+++N E I  E
Sbjct: 1021 AAASRVASCGHSLHRDCLRFYGFGRRVACFRYAAIDVHSVYLPPPKLEFNYDNQEWIQSE 1080

Query: 1081 ANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYI--QELEQIINMEKENFE 1140
            ANEV NRA+ LF EV  AL  + +   G   +D G+   +  I  +ELE ++  ++E F+
Sbjct: 1081 ANEVTNRAEFLFREVYNALQKMSEKLLGPGFQDGGIKSPEKRICIEELEAMLQKDREEFQ 1140

Query: 1141 VHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDI 1200
                EVL  E  VGQP +DI EINKL   +LFLSY+W QRL +A +    +  +  S+ I
Sbjct: 1141 ESLQEVLCKEVKVGQPVIDILEINKLQRQILFLSYVWDQRLIHAFSSIVNNIQEVMSSSI 1200

Query: 1201 PTLEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGRGGNSGKISRSKET 1260
            P L  K + S+E+LVE+ ++    + +   D  S   ++K +I++ + GN+G+IS     
Sbjct: 1201 PKLGLKPVSSVEKLVEINVSPKPSKALSSCD--SALVQTKPDININQEGNTGEISEPGGD 1260

Query: 1261 DREKNIDLG----GEEDDLFLSPVN----SVPLDVGIAIRRANSVGEYPIMTDLSDTLDA 1320
             REK +D       E +       N    S  L+ G  +RRA S GE+PIM +LSDTL+A
Sbjct: 1261 HREKGMDQDLNSRNEAESSLSCSANTSEKSDSLESGKVVRRALSEGEFPIMANLSDTLEA 1320

Query: 1321 VWKGVIHPNSV-IFEDSFSRTNSAILE-PLGGQSDMEKCTANG--VETAHLLHSALVLTK 1380
             W G  HP SV   E+ +S +++ +++      SDM   T++   VE A    SAL    
Sbjct: 1321 AWTGESHPASVGPKENGYSVSDTVVVDLSTAANSDMGNRTSDRGEVEVACSPQSALPTKG 1380

Query: 1381 DDSVETITSSTSMPSSAYNT---KISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMH 1440
             +++E   S  SMP   + +   K S  N  K  ISEY P ++SS RELE+QS  RLL+ 
Sbjct: 1381 PENMEKTMSWASMPFPNFYSLFNKNSSFNAQKLSISEYNPVYVSSLRELERQSGARLLLP 1440

Query: 1441 VGSSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIK 1500
            +G +DTVVPVYD+EPTSIIA++L+  +Y +QMSE EK  +A+D+A S  +  SV +  + 
Sbjct: 1441 IGVNDTVVPVYDDEPTSIIAYALVSSDYYSQMSELEKPKDAADSAVSSSLFDSVNLLLLN 1500

Query: 1501 PCDEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTI 1560
              ++  SD +++  + +E+ILS+  + S    D LL TK+ H RV F ++ P G VK+++
Sbjct: 1501 SFNDSSSDTFRSFGSGDESILSISGSYSSLVSDPLLDTKNFHARVSFTDDGPLGKVKHSV 1560

Query: 1561 TCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIE 1620
            TCY+AK FE+LR+ CCPSELD++RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK E
Sbjct: 1561 TCYYAKWFESLRRTCCPSELDFIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTE 1620

Query: 1621 LESFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFR 1680
            LESFI+F PAYF YLS++I T  PTCLAK++G+YQVSSK+LKGGKE K+DVLV+ENLLFR
Sbjct: 1621 LESFIKFGPAYFKYLSDSISTRSPTCLAKILGIYQVSSKYLKGGKESKMDVLVIENLLFR 1680

Query: 1681 HNVTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDT 1740
             NVTRLYDLKGSSRSRYN D  G NKVLLDQNLIE+MPTSPIF+GSKAKRLLERAVWNDT
Sbjct: 1681 RNVTRLYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGSKAKRLLERAVWNDT 1740

Query: 1741 SFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPT 1787
            SFLA IDVMDYSLLVG+DEEK ELV+GIIDFMRQYTWDKHLETWVK SGILGGPK+A+PT
Sbjct: 1741 SFLALIDVMDYSLLVGVDEEKHELVLGIIDFMRQYTWDKHLETWVKISGILGGPKNASPT 1800

BLAST of Cla002528 vs. TrEMBL
Match: M5W4R3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000098mg PE=4 SV=1)

HSP 1 Score: 1988.0 bits (5149), Expect = 0.0e+00
Identity = 1055/1849 (57.06%), Postives = 1313/1849 (71.01%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MG P+NK+SELV + KSW+P RS+P NVSRDFWMPD+SCRVCY+CDSQFT  NRRHHCR 
Sbjct: 1    MGTPDNKLSELVDIFKSWIPRRSEPPNVSRDFWMPDQSCRVCYDCDSQFTIFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFC+KCTAN +PAPS +    R+D E+IRVCNYCF+QW +  + + +G  A S  +S
Sbjct: 61   CGRVFCAKCTANSVPAPSDEQRAGREDWERIRVCNYCFRQWEQGIATVDNGPPARSPGLS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSG-----------AITDEQD 180
             S S+TS+ STKS  TCHS+ S I STP S G     PY SG           ++T  QD
Sbjct: 121  PSPSATSLASTKSSCTCHSSSSTIGSTPYSTGPYQRVPYTSGLSPNQSSPQIDSVTATQD 180

Query: 181  NLRNAKTINRTTSLMTSSSSYYGY--YRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAV 240
            N  + ++I+   ++  SS +++G+   RSDDEDDDY  G+YR DSE  H S ++DYY AV
Sbjct: 181  NCTSQRSISSDAAMAESSPNHFGFGMNRSDDEDDDY--GVYRLDSEPSHFSHANDYYGAV 240

Query: 241  NTDE--------GLNLERENISTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPSYA 300
            N +E         ++L+ +N S+   P  FDTQ   G++E  E+S E++   EC+   Y 
Sbjct: 241  NIEEFDNVYGPHNVHLDGDNTSSL-LPEGFDTQGVEGSQELREESYEHNNCDECETSPYD 300

Query: 301  MQNKDNEPMDFQNNGILWLPPEPEDEEDEK-ALLFEDDDD-----ACAPGEWGYLHSSLS 360
            +Q+ + EP+DF+NNG+LWLPPEPEDEEDE+ A+LF++DDD       A GEWGYL SS S
Sbjct: 301  LQSTNAEPVDFENNGLLWLPPEPEDEEDEREAVLFDEDDDDGGGVGGATGEWGYLRSSNS 360

Query: 361  --SGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHLS 420
              +GEC  + KS E++R   KNVVEGHFRALV QLL+ E+LP+GDE  ++ WLDIIT LS
Sbjct: 361  FGNGECRTREKSIEEHRNAMKNVVEGHFRALVAQLLQVESLPLGDEDNKESWLDIITSLS 420

Query: 421  WEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKP 480
            WEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I KP
Sbjct: 421  WEAATLLKPDTSKGGGMDPGGYVKVKCIACGRRNESTVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            RF +LGGALEYQRV+N LSSFDTLLQQE+DHLKMAVAKID+HHPNVLLVEKSVSR+AQ+Y
Sbjct: 481  RFLILGGALEYQRVSNLLSSFDTLLQQEMDHLKMAVAKIDSHHPNVLLVEKSVSRYAQDY 540

Query: 541  LLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDG 600
            LL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F E H S  G
Sbjct: 541  LLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLISPKLGYCDIFHVEKFFEVHGSA-G 600

Query: 601  QGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            QGGKK TKTLMFFEGCPKPL  TILLRGA+GDELKKVKHVVQY  FAAYHLALET FLAD
Sbjct: 601  QGGKKLTKTLMFFEGCPKPLGVTILLRGANGDELKKVKHVVQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSGKDAV 720
            EGAS+PELPLKS ITVALP  PS+IDRSIS IPGF+ P+  K   PE   E+ +S K ++
Sbjct: 661  EGASLPELPLKSVITVALPDKPSSIDRSISTIPGFSVPAAGKPQGPEASSELQKSNKGSI 720

Query: 721  SSRDLSIISHNVVNLNS-----------------LEPTLRVKPSATSTSLSFLKQDVSTA 780
            S  DL      ++N+                   +  +  V P +   SLS   +D+  +
Sbjct: 721  SDSDLCTNIDPILNMEGANSICSSKAACSQAFLGVHSSGSVAPRSPFGSLSHPGEDIRDS 780

Query: 781  SRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVSSS-GKGEPSSRYTNTIQI 840
             R  +P    ASE    +  KE  +       EA  +D  +S+S G  E           
Sbjct: 781  FRKKLPGI-CASENDIDMGCKESFLAKTDKAGEALFNDRLISNSFGASEALEHGGGNSHA 840

Query: 841  CRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGT 900
                L AN   +   TS    S N N    +SS+E+FP SPSD Q+ILVSLSTRCVWKGT
Sbjct: 841  DNVDLVANLGEAPGFTSIKNHSDNHNE-EVESSKEEFPPSPSDHQSILVSLSTRCVWKGT 900

Query: 901  ICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISA 960
            +C+R+HL RIKYYG+FDKPLGRFLRD+LFDQ+Y C SCGMPSEAHV+CYTH QGSLTIS 
Sbjct: 901  VCERSHLFRIKYYGNFDKPLGRFLRDHLFDQSYLCRSCGMPSEAHVHCYTHRQGSLTISV 960

Query: 961  KKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNN 1020
            KKLP+  LPGE EGKIWMWHRCL+CPRTNGFPPATRRV+MSDAAWGLSFGKFLELSFSN+
Sbjct: 961  KKLPETLLPGEREGKIWMWHRCLRCPRTNGFPPATRRVVMSDAAWGLSFGKFLELSFSNH 1020

Query: 1021 AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKE 1080
            AAA+RVA CGH LHRDCLRFYGFGR VACF YA+I+V SV++PPS +EF+++N E I KE
Sbjct: 1021 AAANRVATCGHSLHRDCLRFYGFGRMVACFSYASIHVHSVYLPPSKLEFYYDNQEWIQKE 1080

Query: 1081 ANEVHNRAKTLFAEVCKALPLI--PKNESGTEQEDFGVNELDNYIQELEQIINMEKENFE 1140
            A+E+ +RA+ LF E+  AL  I   +  +GT+       E  + I ELE+++  E+E+FE
Sbjct: 1081 ADEMGHRAELLFTELRNALNQILGKRPLAGTQDGGKKAPESSHQIAELEEMLQKEREDFE 1140

Query: 1141 VHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDI 1200
                +V+  E   G P +DI EINKL   +LF SY+W QRL +AA+L  K F +  S+ +
Sbjct: 1141 ESLRKVMHREVKFGHPAIDILEINKLRRQLLFHSYVWDQRLIHAASLSNKGFQEGLSSSL 1200

Query: 1201 PTLEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGRGGNSGKISR--SK 1260
            P L+EK L S+E+L E  + S   + V   D+  L  E+K +I++ +GG+ G  S     
Sbjct: 1201 PKLKEKPLSSMEKLAETNINSKPGKGVSICDSSLL--ETKPDINVNQGGDVGYFSPPGGV 1260

Query: 1261 ETDREKNIDLGGEEDDLFLSPV------NSVPLDVGIAIRRANSVG-EYPIMTDLSDTLD 1320
            +   E  +DL    +    +P        S PL+ G ++RRA S G E P + +LSDTLD
Sbjct: 1261 QNKTEMGLDLNHSNEADLSTPSFPNVIDKSDPLESGKSVRRALSEGDECPTVANLSDTLD 1320

Query: 1321 AVWKGVIHPNSVIFEDS-FSRTNSAI------LEPLGGQSDMEKCTAN--GVETAHLLHS 1380
            A W G  HP S I +D+ +S  +S +      +  +   SD++  T +  GV+  H L S
Sbjct: 1321 AAWTGESHPTSTIPKDNGYSIPDSTLVNSPTAIRKVASNSDLQNYTIDQVGVQVTHSLSS 1380

Query: 1381 ALVLTKDDSVETITSSTSMPSSAYNTKISLLNIPKSDISEYGPAFISSYRELEKQSHLRL 1440
             L L                   ++  ISL N  K  I E  P ++  +RELE+QS  RL
Sbjct: 1381 PLHL-----------------KGFDKNISL-NAQKLFIGEGNPVYVPLFRELERQSGARL 1440

Query: 1441 LMHVGSSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMN 1500
            L+ +G +DTV+PV+D+EPTSIIA++L+ P+Y  Q+SE E+  +A D++ SLP+  S  + 
Sbjct: 1441 LLPIGVNDTVIPVFDDEPTSIIAYALVSPDYHLQISESERPKDALDSSVSLPLFDSANLL 1500

Query: 1501 SIKPCDEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVK 1560
            S+   DE  S+ Y+ L +++E+++S  R+ S Q  D LL +KDLH RV F ++ P G VK
Sbjct: 1501 SLTSFDEAVSETYRNLGSSDESLISTSRSRSSQALDSLL-SKDLHARVSFTDDGPLGKVK 1560

Query: 1561 YTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVT 1620
            YT+TCY+A RFEALR+ CCPSE+D+VRSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVT
Sbjct: 1561 YTVTCYYATRFEALRRTCCPSEIDFVRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVT 1620

Query: 1621 KIELESFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENL 1680
            K ELESFI+FAP+YF YLSE+I T  PTCLAK++G+YQVSSKH KGGKE K+DVLVMENL
Sbjct: 1621 KTELESFIKFAPSYFKYLSESISTRSPTCLAKILGIYQVSSKHGKGGKESKMDVLVMENL 1680

Query: 1681 LFRHNVTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVW 1740
            LFR NVTRLYDLKGSSRSRYN D  G NKVLLDQNLIE+MPTSPIF+G+KAKRLLERAVW
Sbjct: 1681 LFRRNVTRLYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVW 1740

Query: 1741 NDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDA 1783
            NDT+FLASIDVMDYSLLVG+DEEK ELV+GIIDF+RQYTWDKHLETWVK SG+LGGPK+ 
Sbjct: 1741 NDTAFLASIDVMDYSLLVGVDEEKDELVLGIIDFVRQYTWDKHLETWVKTSGLLGGPKNT 1800

BLAST of Cla002528 vs. TrEMBL
Match: A0A061GJU0_THECC (Phosphatidylinositol-4-phosphate 5-kinase family protein, putative isoform 2 (Fragment) OS=Theobroma cacao GN=TCM_037451 PE=4 SV=1)

HSP 1 Score: 1986.8 bits (5146), Expect = 0.0e+00
Identity = 1055/1831 (57.62%), Postives = 1311/1831 (71.60%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MG+P+NK+S+LV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1    MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFC+KCTAN +PAPS      ++DSE+IRVCNYCFKQW +  +A+  G  A S  +S
Sbjct: 61   CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSG----------AITDEQDN 180
             S S+TS+ STKS  TC+S+ S + STP S G  +   Y SG          A   EQ+N
Sbjct: 121  PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181  LRNAKTINRTTSLMTSSSSYYGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
              +  + N +++ + SSS+++G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181  KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241  TD-------------EGLNLERENISTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDF 300
                           +G N++ +++S    P NF+ Q   G ++  E+ +E +   E + 
Sbjct: 241  IGSIDRVYGSDKVHPDGGNMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGEV 300

Query: 301  PSYAMQNKDNEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDACAPGEWGYLHSSLS 360
            P+Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWGYL SS S
Sbjct: 301  PAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSNS 360

Query: 361  --SGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHLS 420
              SGE   + KS+E++R+  KNVVEGHFRALV QLL+ ENLP+GDE G D WLDIIT+LS
Sbjct: 361  FGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYLS 420

Query: 421  WEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKP 480
            WEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I KP
Sbjct: 421  WEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            RF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY
Sbjct: 481  RFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540

Query: 541  LLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDG 600
            LL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  G
Sbjct: 541  LLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA-G 600

Query: 601  QGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            QGGKK TKTLMFF+GCPKPL  TILL+GA+GDELKKVKHVVQY  FAAYHLALET FLAD
Sbjct: 601  QGGKKLTKTLMFFDGCPKPLGYTILLKGANGDELKKVKHVVQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSGKDAV 720
            EGA++PELPLKSPITVALP  P++IDRSIS IPGFT PS  K    +PI E+ +S K  +
Sbjct: 661  EGATLPELPLKSPITVALPDKPASIDRSISTIPGFTVPSSGKPMASQPINELQKSNKVVI 720

Query: 721  SSR---------------DLSIISHNVVNLNSLEP--TLRVKPSATSTSLSFLKQDVSTA 780
            S R                 S +S  +    +L+   +  ++   +  SLS L++++S  
Sbjct: 721  SDRPSSANVEPPCESRGASSSCLSKGLHTQTTLKEYASSSIEAITSLNSLSALRENIS-- 780

Query: 781  SRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVS-SSGKGEPSSRYTNTIQI 840
            S   + S   A      +D KE S+  KT   EA  DD  +S      E   +   +   
Sbjct: 781  SHGNVLSLNHAFSKVNGIDPKE-SVQTKTASSEAVMDDGFISICQSLLEAPDQGGGSNHT 840

Query: 841  CRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGT 900
              N L AN L      S   ++SN N     SS+E+FP SPSD Q+ILVSLSTRCVWKGT
Sbjct: 841  DGNMLVANHLGVPQLASSKRDTSNNNE-EVGSSKEEFPPSPSDHQSILVSLSTRCVWKGT 900

Query: 901  ICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISA 960
            +C+R+HL RIKYYG+FDKPLGRFLRD+LFDQ++RC SC MPSEAHV+CYTH QGSLTIS 
Sbjct: 901  VCERSHLFRIKYYGNFDKPLGRFLRDHLFDQSFRCRSCEMPSEAHVHCYTHRQGSLTISV 960

Query: 961  KKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNN 1020
            +KLP+  LPG+ EGKIWMWHRCL+CPR N FPPATRR++MSDAAWGLSFGKFLELSFSN+
Sbjct: 961  RKLPELPLPGQREGKIWMWHRCLRCPRANKFPPATRRIVMSDAAWGLSFGKFLELSFSNH 1020

Query: 1021 AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKE 1080
            AAASRVA CGH LHRDCLRFYGFGR VACF YAAI+V SV++PP  +EF+++N E I  E
Sbjct: 1021 AAASRVASCGHSLHRDCLRFYGFGRRVACFRYAAIDVHSVYLPPPKLEFNYDNQEWIQSE 1080

Query: 1081 ANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYI--QELEQIINMEKENFE 1140
            ANEV NRA+ LF EV  AL  + +   G   +D G+   +  I  +ELE ++  ++E F+
Sbjct: 1081 ANEVTNRAEFLFREVYNALQKMSEKLLGPGFQDGGIKSPEKRICIEELEAMLQKDREEFQ 1140

Query: 1141 VHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDI 1200
                EVL  E  VGQP +DI EINKL   +LFLSY+W QRL +A +    +  +  S+ I
Sbjct: 1141 ESLQEVLCKEVKVGQPVIDILEINKLQRQILFLSYVWDQRLIHAFSSIVNNIQEVMSSSI 1200

Query: 1201 PTLEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGRGGNSGKISRSKET 1260
            P L  K + S+E+LVE+ ++    + +   D  S   ++K +I++ + GN+G+IS     
Sbjct: 1201 PKLGLKPVSSVEKLVEINVSPKPSKALSSCD--SALVQTKPDININQEGNTGEISEPGGD 1260

Query: 1261 DREKNIDLG----GEEDDLFLSPVN----SVPLDVGIAIRRANSVGEYPIMTDLSDTLDA 1320
             REK +D       E +       N    S  L+ G  +RRA S GE+PIM +LSDTL+A
Sbjct: 1261 HREKGMDQDLNSRNEAESSLSCSANTSEKSDSLESGKVVRRALSEGEFPIMANLSDTLEA 1320

Query: 1321 VWKGVIHPNSV-IFEDSFSRTNSAILE-PLGGQSDMEKCTANG--VETAHLLHSALVLTK 1380
             W G  HP SV   E+ +S +++ +++      SDM   T++   VE A    SAL    
Sbjct: 1321 AWTGESHPASVGPKENGYSVSDTVVVDLSTAANSDMGNRTSDRGEVEVACSPQSALPTKG 1380

Query: 1381 DDSVETITSSTSMPSSAYNT---KISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMH 1440
             +++E   S  SMP   + +   K S  N  K  ISEY P ++SS RELE+QS  RLL+ 
Sbjct: 1381 PENMEKTMSWASMPFPNFYSLFNKNSSFNAQKLSISEYNPVYVSSLRELERQSGARLLLP 1440

Query: 1441 VGSSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIK 1500
            +G +DTVVPVYD+EPTSIIA++L+  +Y +QMSE EK  +A+D+A S  +  SV +  + 
Sbjct: 1441 IGVNDTVVPVYDDEPTSIIAYALVSSDYYSQMSELEKPKDAADSAVSSSLFDSVNLLLLN 1500

Query: 1501 PCDEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTI 1560
              ++  SD +++  + +E+ILS+  + S    D LL TK+ H RV F ++ P G VK+++
Sbjct: 1501 SFNDSSSDTFRSFGSGDESILSISGSYSSLVSDPLLDTKNFHARVSFTDDGPLGKVKHSV 1560

Query: 1561 TCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIE 1620
            TCY+AK FE+LR+ CCPSELD++RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK E
Sbjct: 1561 TCYYAKWFESLRRTCCPSELDFIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTE 1620

Query: 1621 LESFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFR 1680
            LESFI+F PAYF YLS++I T  PTCLAK++G+YQVSSK+LKGGKE K+DVLV+ENLLFR
Sbjct: 1621 LESFIKFGPAYFKYLSDSISTRSPTCLAKILGIYQVSSKYLKGGKESKMDVLVIENLLFR 1680

Query: 1681 HNVTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDT 1740
             NVTRLYDLKGSSRSRYN D  G NKVLLDQNLIE+MPTSPIF+GSKAKRLLERAVWNDT
Sbjct: 1681 RNVTRLYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGSKAKRLLERAVWNDT 1740

Query: 1741 SFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPT 1768
            SFLA IDVMDYSLLVG+DEEK ELV+GIIDFMRQYTWDKHLETWVK SGILGGPK+A+PT
Sbjct: 1741 SFLALIDVMDYSLLVGVDEEKHELVLGIIDFMRQYTWDKHLETWVKISGILGGPKNASPT 1800

BLAST of Cla002528 vs. TrEMBL
Match: A0A067KCV0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07538 PE=4 SV=1)

HSP 1 Score: 1933.7 bits (5008), Expect = 0.0e+00
Identity = 1041/1839 (56.61%), Postives = 1275/1839 (69.33%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MG+P+NKIS+LV  VKSW+P R +P NVS+DFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1    MGNPDNKISDLVDKVKSWIPRRIEPANVSKDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFC+KCTA+ IP PS DP +  +D EKIRVCNYCFKQW +      +G    S  +S
Sbjct: 61   CGRVFCAKCTASSIPVPSDDPRS-GEDWEKIRVCNYCFKQWQQGIPTADNGTSVASPRLS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSGAITD----------EQDN 180
             S S+TS+ STKS  TC+S+ S + ST  S G     P  SG              EQ+N
Sbjct: 121  PSQSATSLVSTKSCCTCNSSTSTLDSTTYSTGAYQCVPCNSGVSPQQFPQMDPTAVEQEN 180

Query: 181  LRNAKTINRTTSL-MTSSSSYYGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAV 240
                ++ + + +  + SS + +GY   RSDDEDD+Y  G+Y S + +RH   +D YY A 
Sbjct: 181  STIGRSADASAAAELYSSENQFGYCMNRSDDEDDEY--GVYHSVAGTRHFFCADGYYGAD 240

Query: 241  NTDE-------------GLNLERENISTKGSPVNFDTQCPRGNEESSEDSDENDKGSECD 300
              DE             G  ++  + S      NFD QC          + E+  G++ +
Sbjct: 241  TVDEIEHMYGPHEMILGGDQIDTSSRSCPPLTENFDAQCADKINNDVAKAYEHG-GNQYE 300

Query: 301  FPSYAMQNKDNEPMDFQNNGILWLPPEPEDEEDEK-ALLFEDDDDA-CAPGEWGYLHSSL 360
             PSY +   + EP+DF+NNG+LWLPPEPEDEED++ A+LF+DDDD     GEWGYL  S 
Sbjct: 301  APSYDLDGANAEPVDFENNGLLWLPPEPEDEEDDREAVLFDDDDDGEITTGEWGYLRPSK 360

Query: 361  S--SGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHL 420
            S  SGE   K KS+ED RK  KNVVEGHFRAL+ QLL+ ENLP+GDE   + WL+IIT L
Sbjct: 361  SFGSGEYRCKDKSNEDNRKAMKNVVEGHFRALIAQLLQVENLPVGDEDDNESWLEIITSL 420

Query: 421  SWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISK 480
            SWEAA +LKPDTSK GGMDPGGYVKVKC+A G+RSES++VKG++CKKNVAHRRM   I K
Sbjct: 421  SWEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRSESLVVKGVVCKKNVAHRRMMSKIDK 480

Query: 481  PRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQE 540
            PRF +LGGALEYQRV+NHLSS DTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSR+AQE
Sbjct: 481  PRFLILGGALEYQRVSNHLSSVDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRYAQE 540

Query: 541  YLLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGD 600
            YLLSK+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  
Sbjct: 541  YLLSKDISLVLNIKRPLLERIARCTGAQIVPSIDHLNSQKLGYCDLFHVEKFLEEHGSA- 600

Query: 601  GQGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLA 660
            GQGGKK TKTLMFFEGCPKPL  TILL+G  GD+LKKVKHVVQY  FAAYHLALET FLA
Sbjct: 601  GQGGKKLTKTLMFFEGCPKPLGYTILLKGGHGDDLKKVKHVVQYGVFAAYHLALETSFLA 660

Query: 661  DEGASVPELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSGKDA 720
            DEGAS+PELP KSPITVALP  PS+IDRSIS IPGFT+P     P  +P+ ++ +S +  
Sbjct: 661  DEGASLPELPFKSPITVALPDKPSSIDRSISIIPGFTAPRTGNQPVSQPLSDVPKSNEG- 720

Query: 721  VSSRDLSIISHNVVNLNSLEPTLRVKPSATSTSLSFLKQDVSTASRNYMPSSKFASEGKR 780
                    +S +V +   ++P  +   SA ST LS  K   S      + SS     G  
Sbjct: 721  --------VSSDVASPTDVKPVYK-SGSADSTCLS--KSPSSQTEFRKLASSSTEHTGSL 780

Query: 781  SLDSK--EYSMGMKTIKEEAGEDDDTVSSSGKGEPSSRYTNTIQICRNSLDANTLSSNDS 840
            +  S   + S G +  K  A  D     S+       + TNT     +SL   + S++ +
Sbjct: 781  TSVSPLGQESSGNRHNKLSADHDSREEDSTQTECFQGKITNT----NDSLFCTSFSTSVA 840

Query: 841  TSQGPESSNA--NVVTTK----------SSREDFPLSPSDQQNILVSLSTRCVWKGTICD 900
              QG +SS A  N++  K          SS+E+FP SPSD Q+ILVSLS+RCVWKGT+C+
Sbjct: 841  LEQGVDSSLADGNILAVKQDGNNQEEIGSSKEEFPPSPSDHQSILVSLSSRCVWKGTVCE 900

Query: 901  RAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISAKKL 960
            RAHL RIKYYGSFDKPLGRFLRD+L DQ+YRC SC MPSEAHV CY H QGSLTIS KKL
Sbjct: 901  RAHLFRIKYYGSFDKPLGRFLRDHLLDQSYRCRSCEMPSEAHVYCYNHRQGSLTISVKKL 960

Query: 961  PDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAA 1020
            P+FFLPGE EGKIWMWHRCL+CPRTNGFPPATRR++MSDAAWGLSFGKFLELSFSN+AAA
Sbjct: 961  PEFFLPGEREGKIWMWHRCLRCPRTNGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAA 1020

Query: 1021 SRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKEANE 1080
            SRVA CGH LHRDCLRFYGFGR VACF YA+I+V SV++PPS +EF+++N E I KE NE
Sbjct: 1021 SRVASCGHSLHRDCLRFYGFGRMVACFRYASIDVHSVYLPPSKLEFNYDNQEWIQKEVNE 1080

Query: 1081 VHNRAKTLFAEVCKALPLIPKNES--GTEQEDFGVNELDNY-IQELEQIINMEKENFEVH 1140
            V  RA  LF EV  +L  I +  S  G++  D   +EL  + + ELE I+   KE FE  
Sbjct: 1081 VRQRADLLFKEVQNSLHRISEKASSVGSQNGDLKASELATFRVVELEGILQKNKEEFEES 1140

Query: 1141 FNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDIPT 1200
            F  + S E  VG+P +DI EINKL   +LF    W + L  A  L      +   + IP 
Sbjct: 1141 FRNMPSKEVKVGRPVIDILEINKLRRQILFNCIAWNETLKDADRLSTTILHEGQRSFIPK 1200

Query: 1201 LEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGRGGNSGKISRSKETDR 1260
            L+EK ++S+E LVE+ L+    +     D  S+P ++K  ++L +GGN   I +      
Sbjct: 1201 LKEKFVNSVENLVEIDLSPKLGKSFSSCD--SVPVDTKPVVNLNQGGNDSYIHQPVRVHE 1260

Query: 1261 EKNIDLG---GEEDDLFLSPVNSV-----PLDVGIAIRRANSVGEYPIMTDLSDTLDAVW 1320
             + +DL     +E    LS   ++     P + G A+RR +S GE+PIM +LSDTLDA W
Sbjct: 1261 GRGMDLDLNLRKEAARSLSSSENINEKSDPSESGNAVRRVHSEGEFPIMDNLSDTLDAAW 1320

Query: 1321 KGVIHPNSVIFEDSFSRT-NSAILEPLGGQSDMEKCTANG--VETAHLLHSALVLTKDDS 1380
             G   P S+  +D+ S   + A +  +   +D+E  T      E  H L SAL      +
Sbjct: 1321 IGKNQPGSMTLKDNGSSLPDPAAVNSVAANTDLENYTIEKGRSEEFHSLDSALGTKGPQN 1380

Query: 1381 VE-TITSSTSMPSSAYNTKISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMHVGSSD 1440
            +E ++      P+     +   L+ PK   S+Y   +I  + ELE+QS  RL + VG+++
Sbjct: 1381 LENSVVMGMPFPNFYSFKRNFSLSSPKLGFSDYNCTYIPLFMELERQSGARLFLRVGNNE 1440

Query: 1441 TVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIKPCDEE 1500
            T+VP+YD+EPTSIIA++L+  +Y  QMSE EK  +A ++  SLP+  S+ + S    DE 
Sbjct: 1441 TIVPIYDDEPTSIIAYTLVSQKYHLQMSEFEKPKDAGESTVSLPLFDSMNLLSFNSFDES 1500

Query: 1501 GSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTITCYFA 1560
             SD Y+++ + EE+ILSMP     Q  D LL+TKDLH RV F +E   G VKY +TCY+A
Sbjct: 1501 ASDIYRSIGSIEESILSMPGFRGSQVLDPLLYTKDLHARVEFTDEDLQGQVKYLVTCYYA 1560

Query: 1561 KRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFI 1620
            KRFEALR+ C  SELD++RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI
Sbjct: 1561 KRFEALRRMCGASELDFIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFI 1620

Query: 1621 QFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFRHNVTR 1680
            +F PAYF YLSE+I TG PTCLAK++G+YQVS+KHLKGGKE K+DVLVMENLLF+ NV R
Sbjct: 1621 KFGPAYFKYLSESISTGSPTCLAKILGIYQVSTKHLKGGKELKMDVLVMENLLFKRNVVR 1680

Query: 1681 LYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLAS 1740
            LYDLKGSSRSRYN D  G NKVLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDTSFLAS
Sbjct: 1681 LYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTSFLAS 1740

Query: 1741 IDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPTVISPH 1781
            IDVMDYSLLVG+DEEK +LV+GIIDFMRQYTWDKHLETWVK SGILGG K+ +PTVISP 
Sbjct: 1741 IDVMDYSLLVGVDEEKHQLVLGIIDFMRQYTWDKHLETWVKASGILGGSKNTSPTVISPQ 1800

BLAST of Cla002528 vs. NCBI nr
Match: gi|659086075|ref|XP_008443752.1| (PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucumis melo])

HSP 1 Score: 3338.9 bits (8656), Expect = 0.0e+00
Identity = 1651/1790 (92.23%), Postives = 1694/1790 (94.64%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MGHPENKIS+LVGLVKSWMPWRSDP NVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFCSKCTANFIPA STDPTN R+DSEKIRVCNYCFKQWGKEPS+LQDGDKATSSA+S
Sbjct: 61   CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSGAITDEQDNLRNAKTINRT 180
            +SSSSTSM STKSGYTCHSAIS+IASTPCS  RQYYDPYCS AI DE DNLRN KTINRT
Sbjct: 121  VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTDEGLNLERENI 240
            TSLMTSSSSY GYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN D GLNLEREN 
Sbjct: 181  TSLMTSSSSYCGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240

Query: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPSYAMQNKDNEPMDFQNNGILWLPP 300
            STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFP+YAMQNKD+EPMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFEDDDDACAPGEWGYLHSSLSSGECPMKGKSSEDYRKTAKNVVEGHFR 360
            EPEDEEDEKALLFEDDDD CAPGEWG+LHSSLSSGECPMKGKSSEDY+KTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFEDDDDVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVCQLLEAENLPIGDEPGEDGWLDIITHLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
            ALV QLLEAENLPIGD P EDGWLDIIT+LSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIASCTGANII 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIA CTGANII
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPL SPITVALPHNPSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  SAIPGFTSPSPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVVNLNSLEPTLRVKPS-A 720
            SAIPGFT P PVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNV NLNSLEPTL +  S A
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLLESNA 720

Query: 721  TSTSLSFLKQDVSTASRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVSSSG 780
            TSTS SFLKQD ST     M SSKF SEGKRSLDSKEYSMGM TIKEEA ED D VSSSG
Sbjct: 721  TSTSFSFLKQDFST-----MLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSG 780

Query: 781  KGEPSSRYTNT-IQICRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQ 840
            K E SSR+ N+ I + RNSLD+NT S NDSTSQGPE+SN ++VTTKSSREDFPLSPSDQQ
Sbjct: 781  KREASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQ 840

Query: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900
            NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH
Sbjct: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900

Query: 901  VNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960
            V+CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW
Sbjct: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960

Query: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020
            GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS
Sbjct: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020

Query: 1021 MIEFHHNNLERINKEANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYIQE 1080
            MIEFH NNLE INKEANEVHNRAKTLF EVCKALPLI KNESG EQEDFG +EL+NYIQE
Sbjct: 1021 MIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQE 1080

Query: 1081 LEQIINMEKENFEVHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATL 1140
            LEQIINMEKENFEVHFN+VLSG TTV QPTVDIFEINKLHMH+LFLSYIWVQRLNY ATL
Sbjct: 1081 LEQIINMEKENFEVHFNKVLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATL 1140

Query: 1141 RKKHFSDSSSNDIPTLEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGR 1200
            RKKH  D SSNDI TLEEKEL+SLEELVELTLASGRDEF GG DNDSLP ESKRE  LGR
Sbjct: 1141 RKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPESKREAGLGR 1200

Query: 1201 GGNSGKISRSKETDREKNIDLGGEEDDLFLSPVNSVPLDVGIAIRRANSVGEYPIMTDLS 1260
             GNSG ISR  ET+REKNIDLGG+EDD  LSPVNS P+DVGI IRRANSVGEYPIMTDLS
Sbjct: 1201 EGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLS 1260

Query: 1261 DTLDAVWKGVIHPNSVIFEDSFSRTNSAILEPLGGQSDMEKCTANGVETAHLLHSALVLT 1320
             TLDAVWKGVIHPNS+IFEDSFSRTNSAILEPL GQSDMEKCTANG+ETAHLLHSALVLT
Sbjct: 1261 GTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAHLLHSALVLT 1320

Query: 1321 KDDSVETITSSTSMPSSAYNTKISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMHVG 1380
             DDSVETIT+STSMP SAYNTKISLLNIPKSDIS+Y   FISSYRELEKQSHLRLLMHVG
Sbjct: 1321 SDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIKPC 1440
            SSDTVVPVYDEEPTSIIA+SLLMPEYIAQMSEP K MEASDTARSLP+IGSVKMNSIK C
Sbjct: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSVKMNSIKTC 1440

Query: 1441 DEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTITC 1500
            DEEGSDAYKTLR+NEETILSMPRTESLQFEDL+  TKDLHTRVCFVNETP GPVKYTITC
Sbjct: 1441 DEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLGPVKYTITC 1500

Query: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
            YFAKRFEALRKKCCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIELE
Sbjct: 1501 YFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFRHN 1620
            SFIQF+ AYFIYLS+AI TGCPTCLAKVVGMYQVSSKHLKGGKE KVDVLVMENLLFRHN
Sbjct: 1561 SFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRHN 1620

Query: 1621 VTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680
            VTRLYDLKGSSRSRYN D  G NKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680

Query: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPTVI 1740
            LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPK+AAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTAYFLMVPDQWPSTTVSPSESHPDLCDDNSEPENSWD 1789
            SPHQYKKRFRKAMT YFLMVPDQWPS+TVSPSESHPDLCD+NSEPENSWD
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWD 1785

BLAST of Cla002528 vs. NCBI nr
Match: gi|778664198|ref|XP_011660241.1| (PREDICTED: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucumis sativus])

HSP 1 Score: 3313.9 bits (8591), Expect = 0.0e+00
Identity = 1637/1790 (91.45%), Postives = 1690/1790 (94.41%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MGHPENKIS+LVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFCS+CTANFIPAPSTD TN R+DSEKIRVCNYCFKQWGKEPS+LQDGDKATSSA+S
Sbjct: 61   CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSGAITDEQDNLRNAKTINRT 180
            LSSSSTSM STKSGYTCHSAIS+IASTPCS GRQYYDPYCS A+ DE DNLRN KTINRT
Sbjct: 121  LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTDEGLNLERENI 240
            TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN DEG NLEREN 
Sbjct: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPSYAMQNKDNEPMDFQNNGILWLPP 300
            STKGSPVNFDTQ PRGNEESSEDSDENDKGSECDFPSYAMQNK++EPMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFEDDDDACAPGEWGYLHSSLSSGECPMKGKSSEDYRKTAKNVVEGHFR 360
            EPEDEEDEKALLFEDDDD CAPGEWGYLHSSLSSGECPMKGKSSEDY+KTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVCQLLEAENLPIGDEPGEDGWLDIITHLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
            ALV QLLEAENLPIGD P EDGWLDIIT+LSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIASCTGANII 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIA CTGANII
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPL SPITVALPHNPSN+DRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNVDRSI 660

Query: 661  SAIPGFTSPSPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVVNLNSLEPTLR-VKPSA 720
            SAIPGFT P PVKSPEPEPIMEIGQSGKDAVSSRD SIISHNVVNLNSLEPT   ++ +A
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSSLLESNA 720

Query: 721  TSTSLSFLKQDVSTASRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVSSSG 780
            TS S SFLKQD ST     M SS F SEGKRSL SKEYSMGM  IKEEAGEDDD VSSSG
Sbjct: 721  TSPSFSFLKQDFST-----MISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDPVSSSG 780

Query: 781  KGEPSSRYTNT-IQICRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQ 840
            K E SSR+ NT + + RNSLDANT S N+STSQGPE+ N ++VTTKSSREDFPLSPSDQQ
Sbjct: 781  KREASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPLSPSDQQ 840

Query: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900
            NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH
Sbjct: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900

Query: 901  VNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960
            V+CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW
Sbjct: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960

Query: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020
            GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS
Sbjct: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020

Query: 1021 MIEFHHNNLERINKEANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYIQE 1080
            MIEFH NNLE INKEANEVHNRAKTLF EVCKALPLIPKNESG EQEDFGV+EL+NYIQE
Sbjct: 1021 MIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDELNNYIQE 1080

Query: 1081 LEQIINMEKENFEVHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATL 1140
            LEQIINMEKENFEVHFN++LSG TTV QPTVDIFEINKLHMH+LFLSYIWVQRLNY ATL
Sbjct: 1081 LEQIINMEKENFEVHFNKLLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATL 1140

Query: 1141 RKKHFSDSSSNDIPTLEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGR 1200
            RKKH  D SSNDI  L EKEL+SLEELVELTLASGRDEF GG DNDSLPAESKRE  LGR
Sbjct: 1141 RKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPAESKREAGLGR 1200

Query: 1201 GGNSGKISRSKETDREKNIDLGGEEDDLFLSPVNSVPLDVGIAIRRANSVGEYPIMTDLS 1260
             GNSG ISR  +TDREKNIDLGG+EDD  LSPVN  P+DVGI IRRANSVGEYPIMT + 
Sbjct: 1201 EGNSGNISRLNKTDREKNIDLGGQEDDFCLSPVNLDPVDVGIGIRRANSVGEYPIMT-VP 1260

Query: 1261 DTLDAVWKGVIHPNSVIFEDSFSRTNSAILEPLGGQSDMEKCTANGVETAHLLHSALVLT 1320
             TLDAVWKGVIHPNS+IF+DSFSRT SAILEPL GQSDMEKCTAN +ETAHLLHSALVLT
Sbjct: 1261 GTLDAVWKGVIHPNSLIFDDSFSRTTSAILEPLAGQSDMEKCTANRIETAHLLHSALVLT 1320

Query: 1321 KDDSVETITSSTSMPSSAYNTKISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMHVG 1380
            +DDSVETI +STSMP SAYNTKISLLNIPKSDIS+Y   FISSYRELEKQSHLRLLMHVG
Sbjct: 1321 RDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIKPC 1440
            SSDTVVPVYDEEPTSIIA+S+LMPEYIAQMSEP K MEASDTARSLP+IGSVKMNSIK C
Sbjct: 1381 SSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSLPVIGSVKMNSIKTC 1440

Query: 1441 DEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTITC 1500
            DEEGSDAYKTLR+NEETILSMPRTESLQFEDL+  +KDLHTRVCF+NETP GPVKYTITC
Sbjct: 1441 DEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFINETPLGPVKYTITC 1500

Query: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
            YFAKRFEALRKKCCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIELE
Sbjct: 1501 YFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFRHN 1620
            SFIQF+ AYFIYLS+AI TGCPTCLAKVVGMYQVSSKHLKGGKE KVDVLVMENLLFRHN
Sbjct: 1561 SFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRHN 1620

Query: 1621 VTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680
            VTRLYDLKGSSRSRYN D  GKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNPDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680

Query: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPTVI 1740
            LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPK+AAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTAYFLMVPDQWPSTTVSPSESHPDLCDDNSEPENSWD 1789
            SPHQYKKRFRKAMT YFLMVPDQWPS+TVSPSESHPDLCD+NSEPENSWD
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWD 1784

BLAST of Cla002528 vs. NCBI nr
Match: gi|778664202|ref|XP_011660242.1| (PREDICTED: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X2 [Cucumis sativus])

HSP 1 Score: 2077.8 bits (5382), Expect = 0.0e+00
Identity = 1024/1164 (87.97%), Postives = 1067/1164 (91.67%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MGHPENKIS+LVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFCS+CTANFIPAPSTD TN R+DSEKIRVCNYCFKQWGKEPS+LQDGDKATSSA+S
Sbjct: 61   CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSGAITDEQDNLRNAKTINRT 180
            LSSSSTSM STKSGYTCHSAIS+IASTPCS GRQYYDPYCS A+ DE DNLRN KTINRT
Sbjct: 121  LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNTDEGLNLERENI 240
            TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN DEG NLEREN 
Sbjct: 181  TSLMTSSSSYYGYYRSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPSYAMQNKDNEPMDFQNNGILWLPP 300
            STKGSPVNFDTQ PRGNEESSEDSDENDKGSECDFPSYAMQNK++EPMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFEDDDDACAPGEWGYLHSSLSSGECPMKGKSSEDYRKTAKNVVEGHFR 360
            EPEDEEDEKALLFEDDDD CAPGEWGYLHSSLSSGECPMKGKSSEDY+KTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFEDDDDVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVCQLLEAENLPIGDEPGEDGWLDIITHLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420
            ALV QLLEAENLPIGD P EDGWLDIIT+LSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIASCTGANII 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIA CTGANII
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPITVALPHNPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPL SPITVALPHNPSN+DRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNVDRSI 660

Query: 661  SAIPGFTSPSPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVVNLNSLEPTLR-VKPSA 720
            SAIPGFT P PVKSPEPEPIMEIGQSGKDAVSSRD SIISHNVVNLNSLEPT   ++ +A
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSSLLESNA 720

Query: 721  TSTSLSFLKQDVSTASRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVSSSG 780
            TS S SFLKQD ST     M SS F SEGKRSL SKEYSMGM  IKEEAGEDDD VSSSG
Sbjct: 721  TSPSFSFLKQDFST-----MISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDPVSSSG 780

Query: 781  KGEPSSRYTNT-IQICRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQ 840
            K E SSR+ NT + + RNSLDANT S N+STSQGPE+ N ++VTTKSSREDFPLSPSDQQ
Sbjct: 781  KREASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPLSPSDQQ 840

Query: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900
            NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH
Sbjct: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900

Query: 901  VNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960
            V+CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW
Sbjct: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960

Query: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020
            GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS
Sbjct: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020

Query: 1021 MIEFHHNNLERINKEANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYIQE 1080
            MIEFH NNLE INKEANEVHNRAKTLF EVCKALPLIPKNESG EQEDFGV+EL+NYIQE
Sbjct: 1021 MIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDELNNYIQE 1080

Query: 1081 LEQIINMEKENFEVHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATL 1140
            LEQIINMEKENFEV    +  G+           +++ L M  L   +   +  +   + 
Sbjct: 1081 LEQIINMEKENFEVSSKHLKGGKEC---------KVDVLVMENLLFRHNVTRLYDLKGSS 1140

Query: 1141 RKKHFSDSSSNDIPTLEEKELDSL 1163
            R ++  D+S  +   L++  ++S+
Sbjct: 1141 RSRYNPDTSGKNKVLLDQNLIESM 1150

BLAST of Cla002528 vs. NCBI nr
Match: gi|778664202|ref|XP_011660242.1| (PREDICTED: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X2 [Cucumis sativus])

HSP 1 Score: 390.2 bits (1001), Expect = 2.0e-104
Identity = 189/199 (94.97%), Postives = 194/199 (97.49%), Query Frame = 1

Query: 1590 YQVSSKHLKGGKEFKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNSDPCGKNKVLLDQNL 1649
            ++VSSKHLKGGKE KVDVLVMENLLFRHNVTRLYDLKGSSRSRYN D  GKNKVLLDQNL
Sbjct: 1087 FEVSSKHLKGGKECKVDVLVMENLLFRHNVTRLYDLKGSSRSRYNPDTSGKNKVLLDQNL 1146

Query: 1650 IESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMR 1709
            IESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMR
Sbjct: 1147 IESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMR 1206

Query: 1710 QYTWDKHLETWVKNSGILGGPKDAAPTVISPHQYKKRFRKAMTAYFLMVPDQWPSTTVSP 1769
            QYTWDKHLETWVKNSGILGGPK+AAPTVISPHQYKKRFRKAMT YFLMVPDQWPS+TVSP
Sbjct: 1207 QYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSP 1266

Query: 1770 SESHPDLCDDNSEPENSWD 1789
            SESHPDLCD+NSEPENSWD
Sbjct: 1267 SESHPDLCDENSEPENSWD 1285

HSP 2 Score: 2008.8 bits (5203), Expect = 0.0e+00
Identity = 1053/1840 (57.23%), Postives = 1312/1840 (71.30%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MG P+ K+SELV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NR+HHCR 
Sbjct: 1    MGTPDKKLSELVDIVKSWIPRRSEPANVSRDFWMPDQSCRVCYECDSQFTVFNRKHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFC+KCTAN IPA S +P + R+D E+IRVCNYC+ QW K  + + +G  A S A+S
Sbjct: 61   CGRVFCAKCTANSIPALSNEPRSGREDWERIRVCNYCYTQWEKGTATVDNGAGAPSPALS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSGAITDEQD----------- 180
             S S+TS+ STKS  TCHS+ S I STP S G   + P CS +++  Q            
Sbjct: 121  PSPSATSLASTKSSCTCHSSNSTIGSTPYSTGPYQHVP-CSSSLSPRQSAKVDSAIVQNG 180

Query: 181  NLRNAKTINRTTSLMTSSSSYYGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAV 240
            N+ + K+IN    +     + +G+   RSDDEDDDY  G YRS SE+RH SQ DDYY AV
Sbjct: 181  NIASQKSINNDAVMEDLLPNQFGFCMNRSDDEDDDY--GAYRSGSETRHFSQVDDYYDAV 240

Query: 241  NTD-------------EGLNLERENISTKGSPV--NFDTQCPRGNEESSEDSDENDKGSE 300
            N D              G N++ +++S+   P+  NFD     G +   E++D +  G +
Sbjct: 241  NVDGVDHVYGPHKMRPNGDNIDSKSLSSS-PPLHENFDKLGVGGVKNVGEETDRHYNGDD 300

Query: 301  CDFPSYAMQNKDNEPMDFQNNGILWLPPEPEDEEDEK-ALLFEDDDD--ACAPGEWGYLH 360
            C+   Y + +   EP+DF+NNG+LWLPPEPEDEEDE+ A LF+DDDD    A GEWGYL 
Sbjct: 301  CEAAPY-VDSTSTEPVDFENNGLLWLPPEPEDEEDEREAALFDDDDDNDEGATGEWGYLR 360

Query: 361  SSLS--SGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDII 420
            SS S  SGE   + KSSE++RK  KNVVEGHFRALV QLL+ ENLP G++   + WL+II
Sbjct: 361  SSSSFGSGEFRSRDKSSEEHRKAMKNVVEGHFRALVSQLLQVENLPAGEDDDRESWLEII 420

Query: 421  THLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRG 480
            T LSWEAA +LKPDTSK GGMDPGGYVKVKC+A G+RSES++VKG++CKKNVAHRRMT  
Sbjct: 421  TSLSWEAATLLKPDTSKGGGMDPGGYVKVKCIACGRRSESMVVKGVVCKKNVAHRRMTSR 480

Query: 481  ISKPRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRH 540
            I KPRF +LGGALEYQRV+N LSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSR+
Sbjct: 481  IDKPRFLILGGALEYQRVSNLLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRY 540

Query: 541  AQEYLLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHD 600
            AQEYLL+K+I+LVLNIKR LLERIA CTGA I+ ++DH+ S KLGYCD F VE F+EEH 
Sbjct: 541  AQEYLLAKDISLVLNIKRPLLERIARCTGAQIVSSIDHLTSPKLGYCDMFHVEKFIEEHG 600

Query: 601  SGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETC 660
            S  GQGGKK  KTLMFFEGCPKP  CTILL+GASGDELKKVKHV+QY  FAAYHLA+ET 
Sbjct: 601  SA-GQGGKKLMKTLMFFEGCPKPFGCTILLKGASGDELKKVKHVIQYGVFAAYHLAMETS 660

Query: 661  FLADEGASVPELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSG 720
            FLADEGAS+PELPLKSPITVALP  PS+IDRSIS +PGF+  +  KSP PE   E+ +S 
Sbjct: 661  FLADEGASLPELPLKSPITVALPDKPSSIDRSISIVPGFSIAATGKSPGPEGASELQKSN 720

Query: 721  KDAVSSRDLSIISHNVVNLNSLEPTLRVK-PSATSTSLSFLKQDVSTASRNYMPSSKFAS 780
            K   S + LS    +++ L   + T     P  +  S+       S      + +  + +
Sbjct: 721  KSFTSDKHLSTNDDHILKLEGYDSTYACTVPCHSPNSIRSNSSFTSLCPTGQLIAESYHN 780

Query: 781  EGKRSLDSKEYSMGMKTIKE-EAGEDDDTVS-SSGKGEPSSRYTNTIQICRNSLDANTLS 840
                     E +   KT+   E   D+  +S SSG  + + +  +      N+L+AN   
Sbjct: 781  GQICPSKCPEETFQAKTVYTGEPVVDNSLISTSSGMSKANGKGVDMSHSDGNALNANCFG 840

Query: 841  SNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIK 900
            + +  S    S++ N     SS+E+FP SPSD Q+ILVSLSTRCVWKGT+C+RAHL RIK
Sbjct: 841  APEVPSVMHYSNHRNE-EVGSSKEEFPPSPSDHQSILVSLSTRCVWKGTVCERAHLFRIK 900

Query: 901  YYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGE 960
            YYGSFDKPLGRFLRD+LFD++Y C SCGM SEAHV+CYTH QGSLTIS KKL +F LPGE
Sbjct: 901  YYGSFDKPLGRFLRDDLFDESYHCRSCGMQSEAHVHCYTHRQGSLTISVKKLQEFLLPGE 960

Query: 961  PEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGH 1020
             EGKIWMWHRCL+CPRTNGFPPATRRV+MSDAAWGLSFGKFLELSFSN+AAASRVA CGH
Sbjct: 961  KEGKIWMWHRCLRCPRTNGFPPATRRVVMSDAAWGLSFGKFLELSFSNHAAASRVASCGH 1020

Query: 1021 FLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKEANEVHNRAKTL 1080
             LHRDCLRFYGFGR VACF YA+I+V SV++PPS +EF++N+L+ I KE NEVH +A+ L
Sbjct: 1021 SLHRDCLRFYGFGRMVACFRYASIDVHSVYLPPSKLEFYYNDLDWIQKEVNEVHKQAELL 1080

Query: 1081 FAEVCKALPLIPKNESGTEQEDFGVN--ELDNYIQELEQIINMEKENFEVHFNEVLSGET 1140
            F EV  AL  I    S T  +D G++  EL   I ELE ++  EKE FE    + L  E 
Sbjct: 1081 FTEVQNALRQISLKISATGSQDGGMSTHELHKQIGELEGMLQKEKEEFEESSRKSLCTEF 1140

Query: 1141 TVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDIPTLEEKELDSL 1200
              G+P +DI EINKL   +LF SY+W QRL +AA++   +  +  S+ +P L+EK ++ +
Sbjct: 1141 KPGKPAIDILEINKLRRQILFSSYVWDQRLVHAASINVNNLQEGLSSSLPKLKEKPINRI 1200

Query: 1201 EELVELTLASGRDEFVGGYDNDSLPAESKREIDLGRGGNSGKISRSKETDREKNIDLG-- 1260
            E++VE+   S   +   G+ +     E   + +L + G++G++ +S    +E    L   
Sbjct: 1201 EKIVEMNSTSKPGK---GFSSCDSFLEKNPDTNLNQQGSAGQLGQSAGVQKENETGLHQS 1260

Query: 1261 -GEEDDLFLSPVNSV-----PLDVGIAIRRANSVGEYPIMTDLSDTLDAVWKGVIHPNSV 1320
             G E DL+LS   ++     P++ G  +RRA S GEYPI+ DLSDTLDA W G  HP S+
Sbjct: 1261 HGNETDLYLSFNENINDKSDPMETGKLVRRALSEGEYPIVPDLSDTLDAAWTGESHPTSI 1320

Query: 1321 I-------FEDSFSRTNSAILEPLGGQSDMEKCTAN--GVETAHLLHSALVLTKDDSVET 1380
                    F DS +  +S +L  +   S++E    +   +E A  + SAL L + +++E 
Sbjct: 1321 ASKEDGHSFADSTTVDSSTVLSMVPANSNVENSAVDLGKIEAARSVGSALPLKRQENIEN 1380

Query: 1381 ITSSTSMPSSAYNTKISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMHVGSSDTVVP 1440
             T    MP    + K   L++ K    +Y P ++S  RELE+QS   LL+ VG +DTVVP
Sbjct: 1381 STRWVGMPFPNSSFKSLPLSVQKLCNGDYNPVYVSLLRELERQSGAWLLLPVGVNDTVVP 1440

Query: 1441 VYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIKPCDEEGSDA 1500
            +YD+EPTSIIA++L+ P+Y +QMSE E++ +A D++ SLP+  SV + S+   DE  +D 
Sbjct: 1441 IYDDEPTSIIAYTLVSPDYHSQMSESERLKDAGDSSVSLPLFDSVNLLSLNSFDESVADT 1500

Query: 1501 YKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTITCYFAKRFE 1560
            Y+ L + +E+ILS+  + S    D L ++KDL  RV F ++ P G VKYT+TCY+AKRFE
Sbjct: 1501 YRGLGSLDESILSVSGSRSSHVVDPLHYSKDLQARVSFTDDGPLGKVKYTVTCYYAKRFE 1560

Query: 1561 ALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAP 1620
            ALR+ CCPSELD++RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+FAP
Sbjct: 1561 ALRRICCPSELDFIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAP 1620

Query: 1621 AYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFRHNVTRLYDL 1680
            AYF YLSE+I +G PTCLAK++G+YQVS+KH+KGGKE K+DVL MENLL+R NVTRLYDL
Sbjct: 1621 AYFRYLSESISSGSPTCLAKILGIYQVSTKHVKGGKESKMDVLAMENLLYRRNVTRLYDL 1680

Query: 1681 KGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVM 1740
            KGSSRSRYN D  G NKVLLDQNLIESMPTSPIF+G+KAKR+LERAVWNDTSFLASIDVM
Sbjct: 1681 KGSSRSRYNPDTSGNNKVLLDQNLIESMPTSPIFVGNKAKRVLERAVWNDTSFLASIDVM 1740

Query: 1741 DYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPTVISPHQYKK 1786
            DYSLLVG+DEEK ELV+GIIDFMRQYTWDKHLE+WVK SG LGGP++  PTVISP QYKK
Sbjct: 1741 DYSLLVGVDEEKHELVLGIIDFMRQYTWDKHLESWVKTSGFLGGPRNTTPTVISPEQYKK 1800

BLAST of Cla002528 vs. NCBI nr
Match: gi|590574827|ref|XP_007012515.1| (Phosphatidylinositol-4-phosphate 5-kinase family protein, putative isoform 1 [Theobroma cacao])

HSP 1 Score: 2006.1 bits (5196), Expect = 0.0e+00
Identity = 1063/1850 (57.46%), Postives = 1325/1850 (71.62%), Query Frame = 1

Query: 1    MGHPENKISELVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60
            MG+P+NK+S+LV +VKSW+P RS+P NVSRDFWMPD+SCRVCYECDSQFT  NRRHHCR 
Sbjct: 1    MGNPDNKLSDLVDIVKSWIPRRSEPPNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPAPSTDPTNVRDDSEKIRVCNYCFKQWGKEPSALQDGDKATSSAIS 120
            CGRVFC+KCTAN +PAPS      ++DSE+IRVCNYCFKQW +  +A+  G  A S  +S
Sbjct: 61   CGRVFCAKCTANSVPAPSDVQRAGQEDSERIRVCNYCFKQWEQWIAAVDTGTNAHSPGLS 120

Query: 121  LSSSSTSMDSTKSGYTCHSAISNIASTPCSNGRQYYDPYCSG----------AITDEQDN 180
             S S+TS+ STKS  TC+S+ S + STP S G  +   Y SG          A   EQ+N
Sbjct: 121  PSPSATSLASTKSSCTCNSSSSTVGSTPYSTGPYHRVNYNSGLSPRESSQMNASATEQNN 180

Query: 181  LRNAKTINRTTSLMTSSSSYYGYY--RSDDEDDDYYYGMYRSDSESRHISQSDDYYCAVN 240
              +  + N +++ + SSS+++G    RSDDEDDDY  G Y SDSESRH + ++DYY A+N
Sbjct: 181  KASGTSTNPSSAAVDSSSNHFGLCDNRSDDEDDDY--GAYHSDSESRHYAHAEDYYGAIN 240

Query: 241  TD-------------EGLNLERENISTKGSPVNFDTQCPRGNEESSEDSDENDKGSECDF 300
                           +G N++ +++S    P NF+ Q   G ++  E+ +E +   E + 
Sbjct: 241  IGSIDRVYGSDKVHPDGGNMDTKSLSGSPLPENFNAQSVDGIKKF-EEVNERENADEGEV 300

Query: 301  PSYAMQNKDNEPMDFQNNGILWLPPEPEDEEDEK--ALLFEDDDDACAPGEWGYLHSSLS 360
            P+Y +   D EP+DF+NNG+LWLPPEPEDEEDE+  AL  +DDDD  A GEWGYL SS S
Sbjct: 301  PAYDVDGTDVEPVDFENNGLLWLPPEPEDEEDERESALFDDDDDDEGASGEWGYLRSSNS 360

Query: 361  --SGECPMKGKSSEDYRKTAKNVVEGHFRALVCQLLEAENLPIGDEPGEDGWLDIITHLS 420
              SGE   + KS+E++R+  KNVVEGHFRALV QLL+ ENLP+GDE G D WLDIIT+LS
Sbjct: 361  FGSGEYRSRDKSNEEHRRAMKNVVEGHFRALVAQLLQVENLPVGDEDGGDSWLDIITYLS 420

Query: 421  WEAAAVLKPDTSKSGGMDPGGYVKVKCVAGGKRSESIIVKGIICKKNVAHRRMTRGISKP 480
            WEAA +LKPDTSK GGMDPGGYVKVKC+A G+R+ES +VKG++CKKNVAHRRMT  I KP
Sbjct: 421  WEAATLLKPDTSKGGGMDPGGYVKVKCIASGRRNESSVVKGVVCKKNVAHRRMTSKIDKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            RF +LGGALEYQR+++HLSSFDTLLQQE+DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY
Sbjct: 481  RFLILGGALEYQRISSHLSSFDTLLQQEMDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540

Query: 541  LLSKNITLVLNIKRSLLERIASCTGANIIPTVDHVASLKLGYCDAFRVETFVEEHDSGDG 600
            LL+K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD F VE F+EEH S  G
Sbjct: 541  LLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLTSPKLGYCDVFHVEKFLEEHGSA-G 600

Query: 601  QGGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            QGGKK TKTLMFF+GCPKPL  TILL+GA+GDELKKVKHVVQY  FAAYHLALET FLAD
Sbjct: 601  QGGKKLTKTLMFFDGCPKPLGYTILLKGANGDELKKVKHVVQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPITVALPHNPSNIDRSISAIPGFTSPSPVKSPEPEPIMEIGQSGKDAV 720
            EGA++PELPLKSPITVALP  P++IDRSIS IPGFT PS  K    +PI E+ +S K  +
Sbjct: 661  EGATLPELPLKSPITVALPDKPASIDRSISTIPGFTVPSSGKPMASQPINELQKSNKVVI 720

Query: 721  SSR---------------DLSIISHNVVNLNSLEP--TLRVKPSATSTSLSFLKQDVSTA 780
            S R                 S +S  +    +L+   +  ++   +  SLS L++++S  
Sbjct: 721  SDRPSSANVEPPCESRGASSSCLSKGLHTQTTLKEYASSSIEAITSLNSLSALRENIS-- 780

Query: 781  SRNYMPSSKFASEGKRSLDSKEYSMGMKTIKEEAGEDDDTVS-SSGKGEPSSRYTNTIQI 840
            S   + S   A      +D KE S+  KT   EA  DD  +S      E   +   +   
Sbjct: 781  SHGNVLSLNHAFSKVNGIDPKE-SVQTKTASSEAVMDDGFISICQSLLEAPDQGGGSNHT 840

Query: 841  CRNSLDANTLSSNDSTSQGPESSNANVVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGT 900
              N L AN L      S   ++SN N     SS+E+FP SPSD Q+ILVSLSTRCVWKGT
Sbjct: 841  DGNMLVANHLGVPQLASSKRDTSNNNE-EVGSSKEEFPPSPSDHQSILVSLSTRCVWKGT 900

Query: 901  ICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVNCYTHPQGSLTISA 960
            +C+R+HL RIKYYG+FDKPLGRFLRD+LFDQ++RC SC MPSEAHV+CYTH QGSLTIS 
Sbjct: 901  VCERSHLFRIKYYGNFDKPLGRFLRDHLFDQSFRCRSCEMPSEAHVHCYTHRQGSLTISV 960

Query: 961  KKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNN 1020
            +KLP+  LPG+ EGKIWMWHRCL+CPR N FPPATRR++MSDAAWGLSFGKFLELSFSN+
Sbjct: 961  RKLPELPLPGQREGKIWMWHRCLRCPRANKFPPATRRIVMSDAAWGLSFGKFLELSFSNH 1020

Query: 1021 AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSMIEFHHNNLERINKE 1080
            AAASRVA CGH LHRDCLRFYGFGR VACF YAAI+V SV++PP  +EF+++N E I  E
Sbjct: 1021 AAASRVASCGHSLHRDCLRFYGFGRRVACFRYAAIDVHSVYLPPPKLEFNYDNQEWIQSE 1080

Query: 1081 ANEVHNRAKTLFAEVCKALPLIPKNESGTEQEDFGVNELDNYI--QELEQIINMEKENFE 1140
            ANEV NRA+ LF EV  AL  + +   G   +D G+   +  I  +ELE ++  ++E F+
Sbjct: 1081 ANEVTNRAEFLFREVYNALQKMSEKLLGPGFQDGGIKSPEKRICIEELEAMLQKDREEFQ 1140

Query: 1141 VHFNEVLSGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLNYAATLRKKHFSDSSSNDI 1200
                EVL  E  VGQP +DI EINKL   +LFLSY+W QRL +A +    +  +  S+ I
Sbjct: 1141 ESLQEVLCKEVKVGQPVIDILEINKLQRQILFLSYVWDQRLIHAFSSIVNNIQEVMSSSI 1200

Query: 1201 PTLEEKELDSLEELVELTLASGRDEFVGGYDNDSLPAESKREIDLGRGGNSGKISRSKET 1260
            P L  K + S+E+LVE+ ++    + +   D  S   ++K +I++ + GN+G+IS     
Sbjct: 1201 PKLGLKPVSSVEKLVEINVSPKPSKALSSCD--SALVQTKPDININQEGNTGEISEPGGD 1260

Query: 1261 DREKNIDLG----GEEDDLFLSPVN----SVPLDVGIAIRRANSVGEYPIMTDLSDTLDA 1320
             REK +D       E +       N    S  L+ G  +RRA S GE+PIM +LSDTL+A
Sbjct: 1261 HREKGMDQDLNSRNEAESSLSCSANTSEKSDSLESGKVVRRALSEGEFPIMANLSDTLEA 1320

Query: 1321 VWKGVIHPNSV-IFEDSFSRTNSAILE-PLGGQSDMEKCTANG--VETAHLLHSALVLTK 1380
             W G  HP SV   E+ +S +++ +++      SDM   T++   VE A    SAL    
Sbjct: 1321 AWTGESHPASVGPKENGYSVSDTVVVDLSTAANSDMGNRTSDRGEVEVACSPQSALPTKG 1380

Query: 1381 DDSVETITSSTSMPSSAYNT---KISLLNIPKSDISEYGPAFISSYRELEKQSHLRLLMH 1440
             +++E   S  SMP   + +   K S  N  K  ISEY P ++SS RELE+QS  RLL+ 
Sbjct: 1381 PENMEKTMSWASMPFPNFYSLFNKNSSFNAQKLSISEYNPVYVSSLRELERQSGARLLLP 1440

Query: 1441 VGSSDTVVPVYDEEPTSIIAFSLLMPEYIAQMSEPEKVMEASDTARSLPIIGSVKMNSIK 1500
            +G +DTVVPVYD+EPTSIIA++L+  +Y +QMSE EK  +A+D+A S  +  SV +  + 
Sbjct: 1441 IGVNDTVVPVYDDEPTSIIAYALVSSDYYSQMSELEKPKDAADSAVSSSLFDSVNLLLLN 1500

Query: 1501 PCDEEGSDAYKTLRTNEETILSMPRTESLQFEDLLLFTKDLHTRVCFVNETPSGPVKYTI 1560
              ++  SD +++  + +E+ILS+  + S    D LL TK+ H RV F ++ P G VK+++
Sbjct: 1501 SFNDSSSDTFRSFGSGDESILSISGSYSSLVSDPLLDTKNFHARVSFTDDGPLGKVKHSV 1560

Query: 1561 TCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIE 1620
            TCY+AK FE+LR+ CCPSELD++RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK E
Sbjct: 1561 TCYYAKWFESLRRTCCPSELDFIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTE 1620

Query: 1621 LESFIQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHLKGGKEFKVDVLVMENLLFR 1680
            LESFI+F PAYF YLS++I T  PTCLAK++G+YQVSSK+LKGGKE K+DVLV+ENLLFR
Sbjct: 1621 LESFIKFGPAYFKYLSDSISTRSPTCLAKILGIYQVSSKYLKGGKESKMDVLVIENLLFR 1680

Query: 1681 HNVTRLYDLKGSSRSRYNSDPCGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDT 1740
             NVTRLYDLKGSSRSRYN D  G NKVLLDQNLIE+MPTSPIF+GSKAKRLLERAVWNDT
Sbjct: 1681 RNVTRLYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGSKAKRLLERAVWNDT 1740

Query: 1741 SFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKDAAPT 1787
            SFLA IDVMDYSLLVG+DEEK ELV+GIIDFMRQYTWDKHLETWVK SGILGGPK+A+PT
Sbjct: 1741 SFLALIDVMDYSLLVGVDEEKHELVLGIIDFMRQYTWDKHLETWVKISGILGGPKNASPT 1800