Cla002337 (gene) Watermelon (97103) v1

NameCla002337
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionCytochrome P450 (AHRD V1 ***- B9I4C8_POPTR); contains Interpro domain(s) IPR001128 Cytochrome P450
LocationChr1 : 4549793 .. 4551943 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTCAATTTGCCAATATCTATGGCCCTCTAATCTCCCTCAAGCTCGGAGCTCAGCGCCTCGTTGTTGCTTCTTCTCCGGCTGCCGCCACCGCAATTCTCAAGACGCAAGACCGCCTTCTGTCTGCTCGATACATCCTTCAAATGACCCCTGATAGAGCTCTCCACGATCACTGTTCTCTTGTTTTCTCCCCTGAATGTGGCGATAGTTGGAAGACTTATAGGTCCATTTGCAAAGCCAATTTATTTACAGCCAAAGCAATTGAATCACAAGCTAATTTGAGAGAGAAGAAGATGAAGGACTTGGTGGAATTTTTAGACTCAAAACAGGGATCTGTTGTGGAGATTAAAGATTTTGTGTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTCTCCAGAGATTTGCTTGGGTTTGTTGGTGATGGATTTAATGGGATTAAAGGGCCTTTCAAGAAAATGATGGATTTGGGTTTGACCCCAAATTTAGCTGATTTCTACCCTTTGTTGAGAAGCTTTGATCTTCAAGGTCTTCGAAAGAAGGCTGCCATTTACAGGAACCAAGTTGATTCTTCGTGGGGAATTCTTATCAAAGAAAGAAGAGAAATCCATTGTCAACAGGGTTCAGCTTCAAGTGATTTCTTGGATGTCTTGATTCAAAGTGGATTCACTGATCAACAAATTAGCTCCTTGGTTATTGTAAGTATAAAAACACTCTTCAACTACTTAAAAACTTAATCGTTTGGGAGCTTCTTGTTTTTTTATGCGTTGGGTTGATGTAGAACGACTCAATTTTGAAAACAATGAATATTAACATTTTGCTTATGTCACATACCACTTCGATTGAGTTTACTACTTTCTCCATCCCTCTCGCTTGTACTCCTTTCTCCCCTTTGGTCCTCATACTTGTGCTAACTTTTTCCGTCCCTCTCTTACTTGCACTTGTCGCCACCGTTTGACCGTTCATCACCCATTTCGACCAATGCTTACACTCCTTTAAGCCATCACGTTCATCATCCGCTCCAACAAACCTCGAACACTTGTGTATTGCAAGTGGGAGAGGATTTGAGGAAGGAGCTCACGCGAGAGAGGAACAGAGAAAGTAGCCAACACAATCTAGTGGATATGTGACGTAAGCAAAATGTTAGTATTAATTATTTTCAAAATCCAGGTCATTCAGCATTAATGCAACACACAATAAATTGGGTCATATGTACGAAAAAAAAAATCTGTTACACTTCATGGCTCATGTTGAGGACTTCCATGATGAAAAAATGAAGTGACCTACTAAATAAACTTGAGTTATTAGTCATCTTCTTGCCAGATAAACTTATGTTCATCTAATACGATATCACATGAAGCCCAAAGCTGTCTCTCTATCTTGTAGGACATATTGTAACAATTGTAAGCTCTCTTCATTTTTTTCAACCTAAGATTCTCAATAAATCCAAATATACAGATGTTAACCTGAACCTCAAATTTTGAGACAAATAAGTTGGTAATGAAGCTTCTAATGCATTATTATTATTGCAGGAAGTGTTATCTGCAGGCACAGATACAACTACGACAACAGTGGAATGGGCAATGACAGAGTTATTGAAGAACAAAGACATATTAAATAAAGTACAAGAAGAAATAAAGAAAGTAACAAAGAAAAGGTTGATCGATGAATCCAAAATTAGTGAGCTTCATTATCTATGGAAATGTGTGAAAGAAACTCTAAGACTTCACCCACCTGCCCCATTCCTTCTTCCACGTTTAGCTCCAGAGGATTGTGAAGTAATGGGATACTCAATCCCAAAAGACACAATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCCAACATTCAATCCAGATAGATTTGGTGTTGGTTGTGATAATGTAGACTTCAAAGGGTATGATTATAGGTATTTGCCATTTGGAGGAGGAAGAAGAATTTGCCCTGGACTTCCAATGGCAATTCTGCAAGTGCCTTTGATTTTAGCCACATTAATTCACAACTTTCAATGGTCACTTCCTAATCATGAGGATTTATCAAACTTGGATTTTAATGGAAGGCTTGGTGTAACATTGCAAAAGGAAGAACCTCTTAAACTTATTCCTAAAAGGAGGATTGTTTGA

mRNA sequence

ATGACTCAATTTGCCAATATCTATGGCCCTCTAATCTCCCTCAAGCTCGGAGCTCAGCGCCTCGTTGTTGCTTCTTCTCCGGCTGCCGCCACCGCAATTCTCAAGACGCAAGACCGCCTTCTGTCTGCTCGATACATCCTTCAAATGACCCCTGATAGAGCTCTCCACGATCACTGTTCTCTTGTTTTCTCCCCTGAATGTGGCGATAGTTGGAAGACTTATAGGTCCATTTGCAAAGCCAATTTATTTACAGCCAAAGCAATTGAATCACAAGCTAATTTGAGAGAGAAGAAGATGAAGGACTTGGTGGAATTTTTAGACTCAAAACAGGGATCTGTTGTGGAGATTAAAGATTTTGTGTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTCTCCAGAGATTTGCTTGGGTTTGTTGGTGATGGATTTAATGGGATTAAAGGGCCTTTCAAGAAAATGATGGATTTGGGTTTGACCCCAAATTTAGCTGATTTCTACCCTTTGTTGAGAAGCTTTGATCTTCAAGGTCTTCGAAAGAAGGCTGCCATTTACAGGAACCAAGTTGATTCTTCGTGGGGAATTCTTATCAAAGAAAGAAGAGAAATCCATTGTCAACAGGGTTCAGCTTCAAGTGATTTCTTGGATGTCTTGATTCAAAGTGGATTCACTGATCAACAAATTAGCTCCTTGGTTATTGAAGTGTTATCTGCAGGCACAGATACAACTACGACAACAGTGGAATGGGCAATGACAGAGTTATTGAAGAACAAAGACATATTAAATAAAGTACAAGAAGAAATAAAGAAAGTAACAAAGAAAAGGTTGATCGATGAATCCAAAATTAGTGAGCTTCATTATCTATGGAAATGTGTGAAAGAAACTCTAAGACTTCACCCACCTGCCCCATTCCTTCTTCCACGTTTAGCTCCAGAGGATTGTGAAGTAATGGGATACTCAATCCCAAAAGACACAATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCCAACATTCAATCCAGATAGATTTGGTGTTGGTTGTGATAATGTAGACTTCAAAGGGTATGATTATAGGTATTTGCCATTTGGAGGAGGAAGAAGAATTTGCCCTGGACTTCCAATGGCAATTCTGCAAGTGCCTTTGATTTTAGCCACATTAATTCACAACTTTCAATGGTCACTTCCTAATCATGAGGATTTATCAAACTTGGATTTTAATGGAAGGCTTGGTGTAACATTGCAAAAGGAAGAACCTCTTAAACTTATTCCTAAAAGGAGGATTGTTTGA

Coding sequence (CDS)

ATGACTCAATTTGCCAATATCTATGGCCCTCTAATCTCCCTCAAGCTCGGAGCTCAGCGCCTCGTTGTTGCTTCTTCTCCGGCTGCCGCCACCGCAATTCTCAAGACGCAAGACCGCCTTCTGTCTGCTCGATACATCCTTCAAATGACCCCTGATAGAGCTCTCCACGATCACTGTTCTCTTGTTTTCTCCCCTGAATGTGGCGATAGTTGGAAGACTTATAGGTCCATTTGCAAAGCCAATTTATTTACAGCCAAAGCAATTGAATCACAAGCTAATTTGAGAGAGAAGAAGATGAAGGACTTGGTGGAATTTTTAGACTCAAAACAGGGATCTGTTGTGGAGATTAAAGATTTTGTGTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTCTCCAGAGATTTGCTTGGGTTTGTTGGTGATGGATTTAATGGGATTAAAGGGCCTTTCAAGAAAATGATGGATTTGGGTTTGACCCCAAATTTAGCTGATTTCTACCCTTTGTTGAGAAGCTTTGATCTTCAAGGTCTTCGAAAGAAGGCTGCCATTTACAGGAACCAAGTTGATTCTTCGTGGGGAATTCTTATCAAAGAAAGAAGAGAAATCCATTGTCAACAGGGTTCAGCTTCAAGTGATTTCTTGGATGTCTTGATTCAAAGTGGATTCACTGATCAACAAATTAGCTCCTTGGTTATTGAAGTGTTATCTGCAGGCACAGATACAACTACGACAACAGTGGAATGGGCAATGACAGAGTTATTGAAGAACAAAGACATATTAAATAAAGTACAAGAAGAAATAAAGAAAGTAACAAAGAAAAGGTTGATCGATGAATCCAAAATTAGTGAGCTTCATTATCTATGGAAATGTGTGAAAGAAACTCTAAGACTTCACCCACCTGCCCCATTCCTTCTTCCACGTTTAGCTCCAGAGGATTGTGAAGTAATGGGATACTCAATCCCAAAAGACACAATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCCAACATTCAATCCAGATAGATTTGGTGTTGGTTGTGATAATGTAGACTTCAAAGGGTATGATTATAGGTATTTGCCATTTGGAGGAGGAAGAAGAATTTGCCCTGGACTTCCAATGGCAATTCTGCAAGTGCCTTTGATTTTAGCCACATTAATTCACAACTTTCAATGGTCACTTCCTAATCATGAGGATTTATCAAACTTGGATTTTAATGGAAGGCTTGGTGTAACATTGCAAAAGGAAGAACCTCTTAAACTTATTCCTAAAAGGAGGATTGTTTGA

Protein sequence

MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALHDHCSLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFVFTSVFNTLSNLIFSRDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLRKKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVLSAGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLRLHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCDNVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGRLGVTLQKEEPLKLIPKRRIV
BLAST of Cla002337 vs. Swiss-Prot
Match: C80B2_COPJA (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 5.6e-106
Identity = 203/437 (46.45%), Postives = 282/437 (64.53%), Query Frame = 1

Query: 3   QFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALHDHCSLV 62
           + A  YG L SLKLG+Q +VVASSPAAA  ILKT D++LS RY+ Q    +  H   S+V
Sbjct: 55  KLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENSIV 114

Query: 63  FSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFVFT 122
           +S EC D+WK  R +C+  LFT K IESQ+ +RE K +++V+FL  K+G VV+I + VF 
Sbjct: 115 WS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFG 174

Query: 123 SVFNTLSNLIFSRDLLGFVGD--GFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 182
           ++ N   NLIFS+D+        G   +K    K++D+G + N AD++P++   DL G R
Sbjct: 175 TLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQR 234

Query: 183 KKAAIYRNQVDSSWGILIKERREIHCQQGSAS-SDFLDVLIQSGFTDQQISSLVIEVLSA 242
           +  A    Q+   WG+++KERR     +GS S +DF+DVL+ +G  DQ+I++L++E+  A
Sbjct: 235 RAVAEVLQQIYDVWGVMLKERRGT---KGSESKNDFVDVLLNAGLDDQKINALLMELFGA 294

Query: 243 GTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLRL 302
           GT+T+ +T+EWA+TEL K   +++K++ E+  V     + ES +  L YL   VKETLRL
Sbjct: 295 GTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRL 354

Query: 303 HPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCDN 362
           HPP P LLPR A E C VM Y+IPK+  I VN W IGRDP  W+D   F P+RF     +
Sbjct: 355 HPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERF--LSSD 414

Query: 363 VDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGRL 422
           VD+KG D+  +PFGGGRRICPGLP+A     LI+ATL+ NF+WSLP     S L  + + 
Sbjct: 415 VDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKF 474

Query: 423 GVTLQKEEPLKLIPKRR 437
           G+TLQK+ PL ++ K R
Sbjct: 475 GLTLQKDPPLLIVLKAR 484

BLAST of Cla002337 vs. Swiss-Prot
Match: C80B1_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica GN=CYP80B1 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 7.4e-106
Identity = 200/438 (45.66%), Postives = 284/438 (64.84%), Query Frame = 1

Query: 3   QFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALHDHCSLV 62
           + A  YG + +LK+G + +VVAS+ +AA+ ILKT DR+LSARY+ Q    +  H   S+V
Sbjct: 54  ELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIV 113

Query: 63  FSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFVFT 122
           +S +C ++WK  R +C+  LFT K IESQA++REKK +++VE+L  KQG  V+I + +F 
Sbjct: 114 WS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFG 173

Query: 123 SVFNTLSNLIFSRDL--LGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 182
           ++ N   NLIFS+++  LG    G +  K    +M++LG + N AD++P+L  FDL G R
Sbjct: 174 TLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQR 233

Query: 183 KKAAIYRNQVDSSWGILIKERREIHCQQGSAS-SDFLDVLIQSGFTDQQISSLVIEVLSA 242
           K+ A     + + WG +++ER+      G  S +DF+DV + SG  D QI++L++E+  A
Sbjct: 234 KEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGA 293

Query: 243 GTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLRL 302
           GT+T+ +T+EWAMTEL KN  I  K++ EI+ V  +R + ES    L YL   VKETLRL
Sbjct: 294 GTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRL 353

Query: 303 HPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCDN 362
           HPP P LLPR A E C ++ Y+IPKD  I VN WGIGRDP  W D  TF+P+RF     +
Sbjct: 354 HPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERF--LNSS 413

Query: 363 VDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGRL 422
           VDF+G D+  +PFG GRRICPGLP+A   + L++AT + N  W LPN   + +L    + 
Sbjct: 414 VDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKF 473

Query: 423 GVTLQKEEPLKLIPKRRI 438
           G+TLQKE PL ++PK R+
Sbjct: 474 GLTLQKEPPLFIVPKSRV 487

BLAST of Cla002337 vs. Swiss-Prot
Match: C80B2_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.1e-105
Identity = 199/438 (45.43%), Postives = 284/438 (64.84%), Query Frame = 1

Query: 3   QFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALHDHCSLV 62
           + A  YG + +LK+G + +VVAS+ +AA+ ILKT DR+LSARY+ Q    +  H   S+V
Sbjct: 55  ELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIV 114

Query: 63  FSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFVFT 122
           +S +C ++WK  R +C+  LFT K IESQA++REKK +++VE+L  KQG  V+I + +F 
Sbjct: 115 WS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFG 174

Query: 123 SVFNTLSNLIFSRDL--LGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 182
           ++ N   NLIFS+++  LG    G +  K    +M++LG + N AD++P+L  FDL G R
Sbjct: 175 TLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQR 234

Query: 183 KKAAIYRNQVDSSWGILIKERREIHCQQGSAS-SDFLDVLIQSGFTDQQISSLVIEVLSA 242
           K+ A     + + WG +++ER+      G  S +DF+DV + SG  D QI++L++E+  A
Sbjct: 235 KEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGA 294

Query: 243 GTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLRL 302
           GT+T+ +T+EWAMTEL KN  I  K++ E++ V  +R + ES    L YL   VKETLRL
Sbjct: 295 GTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRL 354

Query: 303 HPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCDN 362
           HPP P LLPR A E C ++ Y+IPKD  I VN WGIGRDP  W D  TF+P+RF     +
Sbjct: 355 HPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERF--LNSS 414

Query: 363 VDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGRL 422
           VDF+G D+  +PFG GRRICPGLP+A   + L++AT + N  W LPN   + +L    + 
Sbjct: 415 VDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKF 474

Query: 423 GVTLQKEEPLKLIPKRRI 438
           G+TLQKE PL ++PK R+
Sbjct: 475 GLTLQKEPPLFIVPKSRV 488

BLAST of Cla002337 vs. Swiss-Prot
Match: C80B3_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 9.0e-104
Identity = 197/436 (45.18%), Postives = 275/436 (63.07%), Query Frame = 1

Query: 3   QFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALHDHCSLV 62
           Q A  YG L SLKLG++ +VVAS+P AA+ ILKT DR+LS RY+ Q    +  H   S+V
Sbjct: 49  QLAETYGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIV 108

Query: 63  FSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFVFT 122
           +S EC ++WK  R +C+  LFT K IESQA +RE K  ++VE+L    G+ V+I + VF 
Sbjct: 109 WS-ECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFG 168

Query: 123 SVFNTLSNLIFSRDL--LGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 182
           ++ N   NLIFS+++  LG    G   +K    +M++LG + N AD++P L  FDL G  
Sbjct: 169 TLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQS 228

Query: 183 KKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVLSAG 242
           K  A     + S WG ++KER+       S  +DF+++L+ SG  DQQI++L++E+  AG
Sbjct: 229 KDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAG 288

Query: 243 TDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLRLH 302
           T+T+ +T+EWA++EL KN  +   ++ E+  V  KR + ES I  + YL   VKETLRLH
Sbjct: 289 TETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLH 348

Query: 303 PPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCDNV 362
           P  P LLPR A E C+V+ Y+IPK+  I VN WGIGRDP  W D   F+P+RF     ++
Sbjct: 349 PATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERF--LNSSI 408

Query: 363 DFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGRLG 422
           DFKG D+  +PFG GRRICPG+P+A   + LI+++L+ NF W  P   D S L    + G
Sbjct: 409 DFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFG 468

Query: 423 VTLQKEEPLKLIPKRR 437
           +TLQKE PL ++PK R
Sbjct: 469 LTLQKEPPLYIVPKTR 480

BLAST of Cla002337 vs. Swiss-Prot
Match: C80BX_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum GN=CYP80BX PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.8e-91
Identity = 179/384 (46.61%), Postives = 241/384 (62.76%), Query Frame = 1

Query: 3   QFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALHDHCSLV 62
           Q A  YG L +LKLG Q +VVAS+P AA+ +LK  DR L  RY+ Q    +  H   S+V
Sbjct: 55  QLAETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKN-HVENSIV 114

Query: 63  FSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFVFT 122
           ++ EC ++WK  R +C+  LFT K IE+QA +RE K  ++V++L   +G  V+I + +F 
Sbjct: 115 WN-ECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFG 174

Query: 123 SVFNTLSNLIFSRDL--LGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 182
           ++ N   NLIFS+D+  LG    G   +K    +M++LG + N  D+ PLL S DL G R
Sbjct: 175 TLVNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQR 234

Query: 183 KKAAIYRNQVDSSWGILIKERREIHCQ-QGSASSDFLDVLIQSGFTDQQISSLVIEVLSA 242
           K  A     V   WG ++KERR    Q  G   +DF+DVL+ SG  DQQI+SL++++ SA
Sbjct: 235 KDVADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSA 294

Query: 243 GTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLRL 302
           GT+TT +T+EWA+TEL KN  +   ++ E+  V  KR + ES I  L YL   VKETLRL
Sbjct: 295 GTETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRL 354

Query: 303 HPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCDN 362
           HPP P L+PR A E C+V+ Y+IPK+  I VN WGIGRDP  W D   F+PDRF     +
Sbjct: 355 HPPTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRF--LNSS 414

Query: 363 VDFKGYDYRYLPFGGGRRICPGLP 384
           +DFKG D+  +PFG GRRICPG+P
Sbjct: 415 IDFKGNDFELIPFGAGRRICPGVP 434

BLAST of Cla002337 vs. TrEMBL
Match: B9RHX8_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1574420 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 8.7e-130
Identity = 239/440 (54.32%), Postives = 313/440 (71.14%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTP--DRALHDH 60
           M  +A ++GPLISL+LG + +VVASSP AA  ILKT DRLLS RYI   TP  D  L D 
Sbjct: 61  MANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVL-DR 120

Query: 61  CSLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKD 120
            +LV++P C D WK  R++C++ LF+AKAIESQA LREKK+ ++++FL SKQG +V I +
Sbjct: 121 IALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGE 180

Query: 121 FVFTSVFNTLSNLIFSRDLLGFVGDGFNG-IKGPFKKMMDLGLTPNLADFYPLLRSFDLQ 180
            VFT+ FNT+SNL+FS+DLL F   G  G +K     +M+L   PN+ADFYP+L   D Q
Sbjct: 181 VVFTTAFNTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQ 240

Query: 181 GLRKKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVL 240
           G+++K      ++   W I IKERRE H +  +  +DFLDV + +GF D QI+ L++E+ 
Sbjct: 241 GIKRKMKNCLERMFGVWDIYIKERRERHVKD-ARKTDFLDVFLSNGFDDHQINWLLLELF 300

Query: 241 SAGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETL 300
           SAG DTTTTTVEWAM ELLK   +L KV+EE++    K +I ES I +L YL  CVKETL
Sbjct: 301 SAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETL 360

Query: 301 RLHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGC 360
           RLHPP PFL+PR APE CEVM Y+IPK + I VNVW IGRDPS WED  ++ P+RF    
Sbjct: 361 RLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERF--LD 420

Query: 361 DNVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNG 420
            N+DFKG+++ +LPFG GRRICPGLPM   Q+PLILA+L+H F WSL N +D + LD N 
Sbjct: 421 SNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMND 480

Query: 421 RLGVTLQKEEPLKLIPKRRI 438
           +  +TL+KE+ L ++PKR++
Sbjct: 481 KFSITLEKEQHLLVVPKRKL 496

BLAST of Cla002337 vs. TrEMBL
Match: B9R9F5_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1496790 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 1.9e-129
Identity = 231/440 (52.50%), Postives = 318/440 (72.27%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRA-LHDHC 60
           +  FA +YGPLISL+LG Q LVVAS+P++A  +LK  DRLLSAR+IL+  P  + + +  
Sbjct: 60  IANFAKVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERV 119

Query: 61  SLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDF 120
           ++V++P C D WK+ R++C+  LF+ KAIESQA LREKK+ +++EFL +KQG  V + + 
Sbjct: 120 AIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEV 179

Query: 121 VFTSVFNTLSNLIFSRDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGL 180
           VF ++FNT+S+L+FS DL+GF      G+K     MM++  +PN+A+FYP+L   D QGL
Sbjct: 180 VFGTIFNTISHLLFSTDLIGFENQ-IGGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGL 239

Query: 181 RKKAAIYRNQVDSSWGILIKERREIHCQQGSA-SSDFLDVLIQSGFTDQQISSLVIEVLS 240
           ++K      ++   W I IKERR  H    +A  +DFLD+ + +GF D QI+ LV+E+LS
Sbjct: 240 KRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLS 299

Query: 241 AGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLR 300
           AGTDTT+T+VEWAM ELLKNK+ + KV+EE+ +   K  I ES +S+L YL  CVKETLR
Sbjct: 300 AGTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLR 359

Query: 301 LHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCD 360
           LHPPAPFL+PR A E+CEVM Y+IPKD+ + VNVW IGRDPS+WED  +F P+RF +G  
Sbjct: 360 LHPPAPFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERF-LG-S 419

Query: 361 NVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGR 420
           ++D KG+D+  +PFG GRRICPGLPMA  Q+ L+LA+LIH F WSLPN  D + +D   +
Sbjct: 420 SLDVKGHDFELIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEK 479

Query: 421 LGVTLQKEEPLKLIPKRRIV 439
            G+TLQ E PL +IPK +++
Sbjct: 480 FGITLQMEHPLLIIPKPKLL 496

BLAST of Cla002337 vs. TrEMBL
Match: A0A067J8Q3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00026 PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.9e-127
Identity = 230/440 (52.27%), Postives = 309/440 (70.23%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALH--DH 60
           M + A ++GP+ SL+LG Q +VV SSPA A  IL+ +DR LS R++ +  P R  H  D 
Sbjct: 1   MAKLAKLHGPIFSLRLGYQLVVVGSSPAVAAEILRKKDRFLSGRFVPKAIP-RPSHELDR 60

Query: 61  CSLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKD 120
            +LV++P C D WK+ R++ +  LF+AKAIES+A LRE+K+ +++EFL  KQG VV + +
Sbjct: 61  IALVWNPSCSDQWKSLRALSRTELFSAKAIESKAALREQKVSEMIEFLGIKQGQVVNVGE 120

Query: 121 FVFTSVFNTLSNLIFSRDLLGFVGD-GFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQ 180
            VF +VFN++SNL+FS+D + F  +   +G+KG   K+M+LG  PNLADFYP+    DLQ
Sbjct: 121 VVFATVFNSISNLLFSKDFISFEDEESVSGLKGLMWKLMELGSAPNLADFYPIFSGLDLQ 180

Query: 181 GLRKKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVL 240
           GLRKK +    ++   W I IKERRE H + G    DFLDV I +GF D+QI+ L+ E+ 
Sbjct: 181 GLRKKMSKCLKEMFDIWEIFIKERRERHARNGVVKEDFLDVFISNGFDDEQINWLIHELF 240

Query: 241 SAGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETL 300
           SAGTDTTTTTVEWAM ELLKNK+ +NKV +E+++   K  I+ES++S+  YL+ C+KET 
Sbjct: 241 SAGTDTTTTTVEWAMAELLKNKEAMNKVCQELEREINKNSINESQVSQFPYLYACIKETF 300

Query: 301 RLHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGC 360
           RLHPPAPF L     E CEVM Y+IPKD+ I VN+W +GRDP  WED   F P+RF    
Sbjct: 301 RLHPPAPF-LGHQPLETCEVMNYTIPKDSQIIVNIWAVGRDPLAWEDPLLFKPERFLE-- 360

Query: 361 DNVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNG 420
            ++D KG+D+  LPF  GRR+CPGLPMAI  +PLILA+LIH F WSLP  ED + LD N 
Sbjct: 361 SSLDLKGHDFELLPFSSGRRMCPGLPMAIRFLPLILASLIHYFDWSLPGGEDPAMLDMND 420

Query: 421 RLGVTLQKEEPLKLIPKRRI 438
           + G+TLQKE+PL L+P  ++
Sbjct: 421 KYGITLQKEKPLLLVPNTKL 436

BLAST of Cla002337 vs. TrEMBL
Match: B9I4C8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0012s13580g PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 5.8e-126
Identity = 237/439 (53.99%), Postives = 306/439 (69.70%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRA-LHDHC 60
           + + A I+GPLISL+LG Q LVV SS  AA  ILKT DR LSAR++ Q+ P  + +    
Sbjct: 68  LAEIAKIHGPLISLRLGTQLLVVGSSAKAAAEILKTHDRFLSARHVPQVIPRESHVLRRV 127

Query: 61  SLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDF 120
           +LV+ PE  D+WK  R +C+  LF+AKAIES A LREKK+ +L++FL +++G VV I + 
Sbjct: 128 ALVWCPESIDTWKLLRGLCRTELFSAKAIESSATLREKKVGELMDFLVAREGKVVSIGEV 187

Query: 121 VFTSVFNTLSNLIFSRDLLGFVGDGFN-GIKGPFKKMMDLGLTPNLADFYPLLRSFDLQG 180
           VF++VFNT+SNL+FS DL G    G + G+K   +K+M L  TPN+ADFYP+    D QG
Sbjct: 188 VFSTVFNTISNLLFSNDLAGLEEKGMSSGLKSHVRKLMLLVATPNIADFYPIFAGLDPQG 247

Query: 181 LRKKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVLS 240
           LR+K +    +  + W I IKERR  +    S   DFLDV + +GF D QI+ L  E+ S
Sbjct: 248 LRRKLSKLVEETFAIWAINIKERRNSYVHD-SPKRDFLDVFLANGFDDDQINWLAAELFS 307

Query: 241 AGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLR 300
           AGTDTT TT+EWA+ E+LKNK+++ KV EE+++   K  I ES +S L YL  C+KETLR
Sbjct: 308 AGTDTTATTIEWAVAEILKNKEVMKKVDEELEREITKNTISESDVSGLPYLNACIKETLR 367

Query: 301 LHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCD 360
           LHPP P L+P  A E CEVM Y+IPKD+ + VNVW I RDPS WED  +F PDRF +G  
Sbjct: 368 LHPPVPLLVPHRATETCEVMKYTIPKDSQVLVNVWAISRDPSTWEDPLSFKPDRF-LG-S 427

Query: 361 NVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGR 420
           N++FKG +Y +LPFG GRRICPGLPMA   VPLILA+LI  F WSLPN EDL+ LD   +
Sbjct: 428 NLEFKGGNYEFLPFGAGRRICPGLPMANKLVPLILASLIRCFDWSLPNGEDLAKLDMKDK 487

Query: 421 LGVTLQKEEPLKLIPKRRI 438
            GV LQKE+PL L+PKRR+
Sbjct: 488 FGVVLQKEQPLVLVPKRRL 503

BLAST of Cla002337 vs. TrEMBL
Match: M1B4P1_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400014270 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.4e-125
Identity = 225/436 (51.61%), Postives = 320/436 (73.39%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQM-TPDRALHDHC 60
           + QFA IYGPLIS+KLG Q +VVASSP +A  ILKTQDRLLS R + ++ T + ++ D  
Sbjct: 55  LAQFAKIYGPLISVKLGTQLVVVASSPDSAAEILKTQDRLLSVRSVPKVATYELSVIDQH 114

Query: 61  SLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDF 120
           S+VFS +  + WK  R+ C+ +LF+ KA+ESQA LREKK+ +++ FL S++G  V+I + 
Sbjct: 115 SIVFSSDLSNHWKFLRAFCRTHLFSPKAVESQAALREKKVSEMIHFLRSRKGETVKISEI 174

Query: 121 VFTSVFNTLSNLIFSRDLLGFVGD-GFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQG 180
           +F ++ NTL NL FS+DL     +   +GIK   +K ++LG  PN+++FYPL  + DLQG
Sbjct: 175 MFATILNTLGNLFFSKDLCDLDYETNTSGIKHVIRKFVELGAMPNISEFYPLFDALDLQG 234

Query: 181 LRKKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVLS 240
           LRK+  IY+NQ+ + W  ++KE+R+   +   +SSDFLDV+I SGF+D Q+  L++E++S
Sbjct: 235 LRKQTEIYQNQLFNIWSDIVKEKRQAISR---SSSDFLDVMIDSGFSDLQVDILLMELIS 294

Query: 241 AGTDTTTTTVEWAMTELLKNKDILNKVQEEI-KKVTKKRLIDESKISELHYLWKCVKETL 300
           AG+DTTT+T+EWAM ELL+NK  ++K+Q E+  K+ +  ++ ES I+EL YL  CVKETL
Sbjct: 295 AGSDTTTSTIEWAMAELLRNKGAMHKLQAELTSKIGENDILTESNITELPYLAACVKETL 354

Query: 301 RLHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGC 360
           R+HPP PFL+PR APE C++M Y+IPK++ +FVNVW IGR+ + WED+ +F P+RF    
Sbjct: 355 RIHPPTPFLIPRRAPETCKIMDYTIPKNSRLFVNVWAIGRNSNTWEDALSFRPERF--LD 414

Query: 361 DNVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNG 420
            NVDF+G D+ ++PFG GRRICPGLP A  +V LILA+LIH+F+WSLPN ED   +D   
Sbjct: 415 SNVDFRGQDFEFIPFGAGRRICPGLPFARQEVHLILASLIHHFEWSLPNGEDPMQIDMKE 474

Query: 421 RLGVTLQKEEPLKLIP 434
           + GVTLQKE+ L ++P
Sbjct: 475 KFGVTLQKEKSLLVVP 485

BLAST of Cla002337 vs. NCBI nr
Match: gi|659073029|ref|XP_008467223.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 771.9 bits (1992), Expect = 5.8e-220
Identity = 380/441 (86.17%), Postives = 410/441 (92.97%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRALHDHCS 60
           +TQFANIYGPLISLKLGAQRL+VASSPAAATA+LKTQDRLLSARYI QMTPDRALHD  S
Sbjct: 59  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 118

Query: 61  LVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFV 120
           LVFSPECGD WK  RS+CK NLFTAKAIESQA LREKKMK+LV+FL+SKQGSVVE+KDFV
Sbjct: 119 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 178

Query: 121 FTSVFNTLSNLIFSRDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLR 180
           FTSVFNTLSNLIFS DLL FVGDGF GIKGPFKKMMDLGLTPNLADFYPLLR FDLQGL+
Sbjct: 179 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 238

Query: 181 KKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVLSAG 240
            KA IY+N+VDS WGILIKERREIHCQQGSAS+DFLDVLIQSGF DQQI++L+IE+LSAG
Sbjct: 239 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 298

Query: 241 TDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKK-RLIDESKISELHYLWKCVKETLRL 300
           TDTTTTTVEWAM+E+LK+KDILNKV+EE+KKVTK+ R IDES ISEL YLW+CVKETLRL
Sbjct: 299 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 358

Query: 301 HPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGC-- 360
           HPPAPFLLPRLAP DCEVMGYSIPKD+M+FVNVWGIGRDPS+WEDS TFNP RF VGC  
Sbjct: 359 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 418

Query: 361 DNVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNG 420
           DNVDFKGYDYRYLPFGGGRRICPGLPMAI+QVPLILATLIHNF+WSLPN EDLS LD NG
Sbjct: 419 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 478

Query: 421 RLGVTLQKEEPLKLIPKRRIV 439
           RLGVTLQK E LKLIPKRR++
Sbjct: 479 RLGVTLQKYETLKLIPKRRVI 499

BLAST of Cla002337 vs. NCBI nr
Match: gi|778708326|ref|XP_004143707.2| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 673.7 bits (1737), Expect = 2.2e-190
Identity = 334/392 (85.20%), Postives = 358/392 (91.33%), Query Frame = 1

Query: 49  MTPDRALHDHCSLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDS 108
           MTPDRAL++  SLVFSPECGD WK  RSICKANLFTAKAIESQA LREKKMK+LVEFL+S
Sbjct: 1   MTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLES 60

Query: 109 KQGSVVEIKDFVFTSVFNTLSNLIFSRDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFY 168
           KQGSVV+IKDFVFTSVFNTLSNLIFS DLL FVGDGFNGIKG FKKMMDLGLTPNLADFY
Sbjct: 61  KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFY 120

Query: 169 PLLRSFDLQGLRKKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQ 228
           PLLR FDLQGLRKKAAIY N+VDS WGILIKERREIHCQQGSAS+DFLDVLIQSGF+DQQ
Sbjct: 121 PLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFSDQQ 180

Query: 229 ISSLVIEVLSAGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKR-LIDESKISELH 288
           I +L+IE+L A TDTTTTTVEWAM+E+LK+KDILNK +EEIKKV K+   IDES IS+L 
Sbjct: 181 IDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQ 240

Query: 289 YLWKCVKETLRLHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPT 348
           YLW CVKETLRLHPP PFLLPRLAP DCEVMGYSIPKD+MIFVNVWGIGRDPSIWEDS T
Sbjct: 241 YLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300

Query: 349 FNPDRFGVGC-DNVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPN 408
           FNP+RF VGC +NVDFKGYDYRYLPFGGGRRICPGLPMAI+QVPLILATL+HNF+WSLPN
Sbjct: 301 FNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPN 360

Query: 409 HEDLSNLDFNGRLGVTLQKEEPLKLIPKRRIV 439
            EDLS LD N RLGVTLQK E LKLIPKRR+V
Sbjct: 361 GEDLSKLDLNERLGVTLQKYETLKLIPKRRLV 392

BLAST of Cla002337 vs. NCBI nr
Match: gi|743896671|ref|XP_011041604.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Populus euphratica])

HSP 1 Score: 486.9 bits (1252), Expect = 3.8e-134
Identity = 247/436 (56.65%), Postives = 319/436 (73.17%), Query Frame = 1

Query: 4   FANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTP-DRALHDHCSLV 63
           FA  +GPLISLKLG Q L+V SS  AAT ILK+ DRLLSARY+ +    +  + D  S+V
Sbjct: 60  FAKTHGPLISLKLGKQLLIVGSSKRAATEILKSHDRLLSARYVFKAALFENHVLDRISIV 119

Query: 64  FSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDFVFT 123
           ++ +C D WK+ R++C+  LF+AKAIESQA LREKKM ++VEF+  ++G VV I + V  
Sbjct: 120 WATQCSDGWKSLRALCRNELFSAKAIESQAVLREKKMGEMVEFIGRRKGEVVGIGEVVLA 179

Query: 124 SVFNTLSNLIFSRDLLGFVGDGF-NGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGLRK 183
            VFNT++NL+FS DL+G   DG   G+K    +MM LG TPN++DFYP+L   D QGL++
Sbjct: 180 IVFNTIANLLFSVDLIGLEDDGAATGLKSLMWRMMKLGATPNISDFYPILGGIDPQGLKR 239

Query: 184 KAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVLSAGT 243
           K A+  NQ+   WG  IKERRE H   G  S DFLDV + +GF D QI+ L +E+LSAGT
Sbjct: 240 KMAVCVNQMFGIWGKYIKERREKHVHDGPRS-DFLDVFLANGFEDLQINWLALELLSAGT 299

Query: 244 DTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLRLHP 303
           DTT TTVEWA+ ELLKNK++L KV EEIK+ T    + ES +S+L YL  CV ETLRLHP
Sbjct: 300 DTTATTVEWAIAELLKNKEVLKKVSEEIKRETDTNSLKESHVSQLPYLNACVNETLRLHP 359

Query: 304 PAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCDNVD 363
           P PFL+PR A E C+VM Y+IP+D+ + VNVW +GRDPS+WED  +F P+RF +G D +D
Sbjct: 360 PVPFLIPRRALETCKVMDYTIPRDSEVIVNVWAVGRDPSLWEDPLSFKPERF-LGSD-LD 419

Query: 364 FKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGRLGV 423
           FKG+D+ +LPFG GRRICPGLPMA  QV LI+ATL++ F WSLPN ED + LD + + G+
Sbjct: 420 FKGHDFEFLPFGAGRRICPGLPMAAKQVHLIIATLLYYFDWSLPNGEDPATLDMSEKFGI 479

Query: 424 TLQKEEPLKLIPKRRI 438
           TLQKE+PL L+P+RRI
Sbjct: 480 TLQKEQPLLLVPRRRI 492

BLAST of Cla002337 vs. NCBI nr
Match: gi|255544572|ref|XP_002513347.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Ricinus communis])

HSP 1 Score: 471.9 bits (1213), Expect = 1.3e-129
Identity = 239/440 (54.32%), Postives = 313/440 (71.14%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTP--DRALHDH 60
           M  +A ++GPLISL+LG + +VVASSP AA  ILKT DRLLS RYI   TP  D  L D 
Sbjct: 61  MANYAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVL-DR 120

Query: 61  CSLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKD 120
            +LV++P C D WK  R++C++ LF+AKAIESQA LREKK+ ++++FL SKQG +V I +
Sbjct: 121 IALVWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGE 180

Query: 121 FVFTSVFNTLSNLIFSRDLLGFVGDGFNG-IKGPFKKMMDLGLTPNLADFYPLLRSFDLQ 180
            VFT+ FNT+SNL+FS+DLL F   G  G +K     +M+L   PN+ADFYP+L   D Q
Sbjct: 181 VVFTTAFNTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQ 240

Query: 181 GLRKKAAIYRNQVDSSWGILIKERREIHCQQGSASSDFLDVLIQSGFTDQQISSLVIEVL 240
           G+++K      ++   W I IKERRE H +  +  +DFLDV + +GF D QI+ L++E+ 
Sbjct: 241 GIKRKMKNCLERMFGVWDIYIKERRERHVKD-ARKTDFLDVFLSNGFDDHQINWLLLELF 300

Query: 241 SAGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETL 300
           SAG DTTTTTVEWAM ELLK   +L KV+EE++    K +I ES I +L YL  CVKETL
Sbjct: 301 SAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETL 360

Query: 301 RLHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGC 360
           RLHPP PFL+PR APE CEVM Y+IPK + I VNVW IGRDPS WED  ++ P+RF    
Sbjct: 361 RLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERF--LD 420

Query: 361 DNVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNG 420
            N+DFKG+++ +LPFG GRRICPGLPM   Q+PLILA+L+H F WSL N +D + LD N 
Sbjct: 421 SNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMND 480

Query: 421 RLGVTLQKEEPLKLIPKRRI 438
           +  +TL+KE+ L ++PKR++
Sbjct: 481 KFSITLEKEQHLLVVPKRKL 496

BLAST of Cla002337 vs. NCBI nr
Match: gi|255539531|ref|XP_002510830.1| (PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus communis])

HSP 1 Score: 470.7 bits (1210), Expect = 2.8e-129
Identity = 231/440 (52.50%), Postives = 318/440 (72.27%), Query Frame = 1

Query: 1   MTQFANIYGPLISLKLGAQRLVVASSPAAATAILKTQDRLLSARYILQMTPDRA-LHDHC 60
           +  FA +YGPLISL+LG Q LVVAS+P++A  +LK  DRLLSAR+IL+  P  + + +  
Sbjct: 60  IANFAKVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERV 119

Query: 61  SLVFSPECGDSWKTYRSICKANLFTAKAIESQANLREKKMKDLVEFLDSKQGSVVEIKDF 120
           ++V++P C D WK+ R++C+  LF+ KAIESQA LREKK+ +++EFL +KQG  V + + 
Sbjct: 120 AIVWNPACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEV 179

Query: 121 VFTSVFNTLSNLIFSRDLLGFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRSFDLQGL 180
           VF ++FNT+S+L+FS DL+GF      G+K     MM++  +PN+A+FYP+L   D QGL
Sbjct: 180 VFGTIFNTISHLLFSTDLIGFENQ-IGGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGL 239

Query: 181 RKKAAIYRNQVDSSWGILIKERREIHCQQGSA-SSDFLDVLIQSGFTDQQISSLVIEVLS 240
           ++K      ++   W I IKERR  H    +A  +DFLD+ + +GF D QI+ LV+E+LS
Sbjct: 240 KRKMTKCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLS 299

Query: 241 AGTDTTTTTVEWAMTELLKNKDILNKVQEEIKKVTKKRLIDESKISELHYLWKCVKETLR 300
           AGTDTT+T+VEWAM ELLKNK+ + KV+EE+ +   K  I ES +S+L YL  CVKETLR
Sbjct: 300 AGTDTTSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLR 359

Query: 301 LHPPAPFLLPRLAPEDCEVMGYSIPKDTMIFVNVWGIGRDPSIWEDSPTFNPDRFGVGCD 360
           LHPPAPFL+PR A E+CEVM Y+IPKD+ + VNVW IGRDPS+WED  +F P+RF +G  
Sbjct: 360 LHPPAPFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERF-LG-S 419

Query: 361 NVDFKGYDYRYLPFGGGRRICPGLPMAILQVPLILATLIHNFQWSLPNHEDLSNLDFNGR 420
           ++D KG+D+  +PFG GRRICPGLPMA  Q+ L+LA+LIH F WSLPN  D + +D   +
Sbjct: 420 SLDVKGHDFELIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEK 479

Query: 421 LGVTLQKEEPLKLIPKRRIV 439
            G+TLQ E PL +IPK +++
Sbjct: 480 FGITLQMEHPLLIIPKPKLL 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C80B2_COPJA5.6e-10646.45Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica GN=C... [more]
C80B1_ESCCA7.4e-10645.66(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
C80B2_ESCCA2.1e-10545.43(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=C... [more]
C80B3_PAPSO9.0e-10445.18(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
C80BX_PAPSO1.8e-9146.61(S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum GN=CYP8... [more]
Match NameE-valueIdentityDescription
B9RHX8_RICCO8.7e-13054.32(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=R... [more]
B9R9F5_RICCO1.9e-12952.50(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=R... [more]
A0A067J8Q3_JATCU6.9e-12752.27Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00026 PE=3 SV=1[more]
B9I4C8_POPTR5.8e-12653.99Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0012s13580g PE=3 SV=1[more]
M1B4P1_SOLTU8.4e-12551.61Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400014270 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
gi|659073029|ref|XP_008467223.1|5.8e-22086.17PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... [more]
gi|778708326|ref|XP_004143707.2|2.2e-19085.20PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sat... [more]
gi|743896671|ref|XP_011041604.1|3.8e-13456.65PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Populus eup... [more]
gi|255544572|ref|XP_002513347.1|1.3e-12954.32PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Ricinus com... [more]
gi|255539531|ref|XP_002510830.1|2.8e-12952.50PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus communis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043666 regulation of phosphoprotein phosphatase activity
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009966 regulation of signal transduction
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0004864 protein phosphatase inhibitor activity
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla002337Cla002337.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 370..379
score: 1.1E-12coord: 379..390
score: 1.1E-12coord: 239..256
score: 1.1E-12coord: 291..302
score: 1.1
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 1..436
score: 2.6E
IPR001128Cytochrome P450PFAMPF00067p450coord: 1..424
score: 4.7
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..436
score: 1.31E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 331..355
score: 2.1E-35coord: 290..308
score: 2.1E-35coord: 5..24
score: 2.1E-35coord: 248..274
score: 2.1E-35coord: 369..379
score: 2.1E-35coord: 228..245
score: 2.1E-35coord: 379..402
score: 2.1
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 372..381
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..438
score: 1.3E
NoneNo IPR availablePANTHERPTHR24298:SF122CYTOCHROME P450 MONOOXYGENASE-LIKE PROTEINcoord: 1..438
score: 1.3E