Cla002325 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla002325
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Aspartyl protease family protein (AHRD V1 **-- Q9LI73_ARATH); contains Interpro domain(s) IPR001461 Peptidase A1
Location	Chr1 : 4730607 .. 4731182 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCGGCGGCGGCCTCCGCAGCCTTCCCGTGAACAATGCCACCAGAATCAGCTACACCCCCTTGCAGATTAACCCTCTCTCCCCCACATTCTACTACATTGGCATCCACAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCCGCCGTATGGGCAATCGACGAGCAGGGAAACGGCGGTACGGTGGTCGATTCAGGGACGACATTGACCTACCTGGCGAAGGCGGCGTACGAGGAGGTGCTGAAGGCGGTAAGACAGCGAGTGAAACTACCAAATGCAGCTGAGTTGACTCCGGGGTTCGATCTATGCGTGAACGCGTCAGGGGAGTCACGGCGGCCGAGTCTGCCGCGACTGAGATTGGAACTGAAGGGTGGGGCGGTGTTTGCGCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGAGAGTGATGTGCTTGGCGATCCGAGCGGTGGAATCCGGTAATGGGTTTTCGGTGATTGGGAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAGGGAGGCGTCGAGGCTGGGTTTTTCAAGGCGGGGTTGTGGCCTTCCATGA

mRNA sequence

ATGATCGGCGGCGGCCTCCGCAGCCTTCCCGTGAACAATGCCACCAGAATCAGCTACACCCCCTTGCAGATTAACCCTCTCTCCCCCACATTCTACTACATTGGCATCCACAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCCGCCGTATGGGCAATCGACGAGCAGGGAAACGGCGGTACGGTGGTCGATTCAGGGACGACATTGACCTACCTGGCGAAGGCGGCGTACGAGGAGGTGCTGAAGGCGGTAAGACAGCGAGTGAAACTACCAAATGCAGCTGAGTTGACTCCGGGGTTCGATCTATGCGTGAACGCGTCAGGGGAGTCACGGCGGCCGAGTCTGCCGCGACTGAGATTGGAACTGAAGGGTGGGGCGGTGTTTGCGCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGAGAGTGATGTGCTTGGCGATCCGAGCGGTGGAATCCGGTAATGGGTTTTCGGTGATTGGGAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAGGGAGGCGTCGAGGCTGGGTTTTTCAAGGCGGGGTTGTGGCCTTCCATGA

Coding sequence (CDS)

ATGATCGGCGGCGGCCTCCGCAGCCTTCCCGTGAACAATGCCACCAGAATCAGCTACACCCCCTTGCAGATTAACCCTCTCTCCCCCACATTCTACTACATTGGCATCCACAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCCGCCGTATGGGCAATCGACGAGCAGGGAAACGGCGGTACGGTGGTCGATTCAGGGACGACATTGACCTACCTGGCGAAGGCGGCGTACGAGGAGGTGCTGAAGGCGGTAAGACAGCGAGTGAAACTACCAAATGCAGCTGAGTTGACTCCGGGGTTCGATCTATGCGTGAACGCGTCAGGGGAGTCACGGCGGCCGAGTCTGCCGCGACTGAGATTGGAACTGAAGGGTGGGGCGGTGTTTGCGCCGCCGCCGAGGAACTATTTTCTGGAAACGGAGGAGAGAGTGATGTGCTTGGCGATCCGAGCGGTGGAATCCGGTAATGGGTTTTCGGTGATTGGGAATCTGATGCAGCAAGGATTCTTGTTGGAGTTCGATAGGGAGGCGTCGAGGCTGGGTTTTTCAAGGCGGGGTTGTGGCCTTCCATGA

Protein sequence

MIGGGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGCGLP

Homology

[Close]

BLAST of Cla002325 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.6e-23
Identity = 56/162 (34.57%), Postives = 86/162 (53.09%), Query Frame = 1


Query: 29  PTFYYIGIHSITVDGVKLPINPAVWAIDEQ-GNGGTVVDSGTTLTYLAKAAYEEVLKAVR 88
           PTFYYI ++ ++V   +LPI+P+ +A++   G GG ++DSGTTLTY    AY+ V +   
Sbjct: 277 PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFI 336

Query: 89  QRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNYFLETEERVMC 148
            ++ LP     + GFDLC     +     +P   +   GG +   P  NYF+     ++C
Sbjct: 337 SQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDL-ELPSENYFISPSNGLIC 396

Query: 149 LAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGCG 190
           LA+ +  S  G S+ GN+ QQ  L+ +D   S + F+   CG
Sbjct: 397 LAMGS--SSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCG 435

[Close]

BLAST of Cla002325 vs. Swiss-Prot
Match: APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.5e-20
Identity = 60/172 (34.88%), Postives = 91/172 (52.91%), Query Frame = 1


Query: 19  YTPLQINPLSPTFYYIGIHSITVDGVKLP-INPAVWAIDEQGNGGTVVDSGTTLTYLAKA 78
           +TPL  NP   TFYY+G+  I+V G ++P +  +++ +D+ GNGG ++DSGT++T L + 
Sbjct: 317 FTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRP 376

Query: 79  AYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNY 138
           AY  +  A R   K    A     FD C + S       +P + L  +G  V + P  NY
Sbjct: 377 AYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLS-NMNEVKVPTVVLHFRGADV-SLPATNY 436

Query: 139 FLETEER-VMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGC 189
            +  +     C A     +  G S+IGN+ QQGF + +D  +SR+GF+  GC
Sbjct: 437 LIPVDTNGKFCFAFAG--TMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Cla002325 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.0e-20
Identity = 56/170 (32.94%), Postives = 85/170 (50.00%), Query Frame = 1


Query: 20  TPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYLAKAAY 79
           T L  + L+PT+YYI +  ITV G  L I  + + + + G GG ++DSGTTLTYL + AY
Sbjct: 269 TTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAY 328

Query: 80  EEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNYFL 139
             V +A   ++ LP   E + G   C     +     +P + ++  GG V     +N  +
Sbjct: 329 NAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILI 388

Query: 140 ETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGCG 190
              E V+CLA+ +  S  G S+ GN+ QQ   + +D +   + F    CG
Sbjct: 389 SPAEGVICLAMGS-SSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCG 436

[Close]

BLAST of Cla002325 vs. Swiss-Prot
Match: ASPG2_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.0e-19
Identity = 63/186 (33.87%), Postives = 93/186 (50.00%), Query Frame = 1


Query: 5   GLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTV 64
           G  +LPV      S+ PL  NP +P+FYY+G+  + V GV++P+   V+ + E G+GG V
Sbjct: 293 GREALPVG----ASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVV 352

Query: 65  VDSGTTLTYLAKAAYEEVLKAVR-QRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLE 124
           +D+GT +T L  AAY       + Q   LP A+ ++  FD C + SG      +P +   
Sbjct: 353 MDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FDTCYDLSG-FVSVRVPTVSFY 412

Query: 125 LKGGAVFAPPPRNYFLETEER-VMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLG 184
              G V   P RN+ +  ++    C A  A  S  G S+IGN+ Q+G  + FD     +G
Sbjct: 413 FTEGPVLTLPARNFLMPVDDSGTYCFAFAA--SPTGLSIIGNIQQEGIQVSFDGANGFVG 470

Query: 185 FSRRGC 189
           F    C
Sbjct: 473 FGPNVC 470

[Close]

BLAST of Cla002325 vs. Swiss-Prot
Match: ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.8e-15
Identity = 51/170 (30.00%), Postives = 79/170 (46.47%), Query Frame = 1


Query: 21  PLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYLAKAAYE 80
           PL  N    TFYY+G+   +V G K+ +  A++ +D  G+GG ++D GT +T L   AY 
Sbjct: 334 PLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYN 393

Query: 81  EVLKA-VRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNYFL 140
            +  A ++  V L   +     FD C + S  S    +P +     GG     P +NY +
Sbjct: 394 SLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLS-TVKVPTVAFHFTGGKSLDLPAKNYLI 453

Query: 141 ETEER-VMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGC 189
             ++    C A     S    S+IGN+ QQG  + +D   + +G S   C
Sbjct: 454 PVDDSGTFCFAFAPTSS--SLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Cla002325 vs. TrEMBL
Match: A0A0A0KNH6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 1.5e-94
Identity = 173/191 (90.58%), Postives = 180/191 (94.24%), Query Frame = 1


Query: 1   MIGGGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGN 60
           MIGGGL SLP+ NAT+ISYTPLQINPLSPTFYYI IHSIT+DGVKLPINPAVW IDEQGN
Sbjct: 269 MIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQGN 328

Query: 61  GGTVVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRL 120
           GGTVVDSGTTLTYL K AYEEVLK+VR+RVKLPNAAELTPGFDLCVNASGESRRPSLPRL
Sbjct: 329 GGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRL 388

Query: 121 RLELKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASR 180
           R  L GGAVFAPPPRNYFLETEE VMCLAIRAVESGNGFSVIGNLMQQGFLLEFD+E SR
Sbjct: 389 RFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESR 448

Query: 181 LGFSRRGCGLP 192
           LGF+RRGCGLP
Sbjct: 449 LGFTRRGCGLP 459

[Close]

BLAST of Cla002325 vs. TrEMBL
Match: A0A067K2U7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18279 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.2e-67
Identity = 124/188 (65.96%), Postives = 154/188 (81.91%), Query Frame = 1


Query: 4   GGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGT 63
           GG ++  V+    +++TPL +N LSPTFYYIGI S++VDGVKLPINP+VW+ID+ GNGGT
Sbjct: 269 GGHQNSAVSRKRILNFTPLLVNSLSPTFYYIGIKSVSVDGVKLPINPSVWSIDDLGNGGT 328

Query: 64  VVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLE 123
           ++DSGTTLT+L + AY E+L A+++RVKLP   ELTPGFDLCVN SG  RRP  PR+ LE
Sbjct: 329 IIDSGTTLTFLVEPAYREILSAIKRRVKLPGPGELTPGFDLCVNVSG-VRRPVFPRMSLE 388

Query: 124 LKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGF 183
           L G +VF+PPPRNYF++T E V CLAI+ V SG+GFSVIGNLMQQG+LLEFDR+ SRLGF
Sbjct: 389 LAGNSVFSPPPRNYFIDTSEGVKCLAIQPVNSGSGFSVIGNLMQQGYLLEFDRDRSRLGF 448

Query: 184 SRRGCGLP 192
           +R GC LP
Sbjct: 449 ARSGCALP 455

[Close]

BLAST of Cla002325 vs. TrEMBL
Match: B9SEI2_RICCO (Basic 7S globulin 2 small subunit, putative OS=Ricinus communis GN=RCOM_0705030 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 8.6e-66
Identity = 119/188 (63.30%), Postives = 154/188 (81.91%), Query Frame = 1


Query: 4   GGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGT 63
           GG +++ V+    +S+TPL INPLSPTFYYI I  + V+GVKLPINP+VW+ID+ GNGGT
Sbjct: 269 GGAQNVAVSKKGIMSFTPLLINPLSPTFYYIAIKGVYVNGVKLPINPSVWSIDDLGNGGT 328

Query: 64  VVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLE 123
           ++DSGTTLT++ + AY E+LKA ++RVKLP+ AE TPGFDLC+N SG + RP+LPR+   
Sbjct: 329 IIDSGTTLTFITEPAYTEILKAFKKRVKLPSPAEPTPGFDLCMNVSGVT-RPALPRMSFN 388

Query: 124 LKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGF 183
           L GG+VF+PPPRNYF+ET +++ CLA++ V    GFSV+GNLMQQGFLLEFDR+ SRLGF
Sbjct: 389 LAGGSVFSPPPRNYFIETGDQIKCLAVQPVSQDGGFSVLGNLMQQGFLLEFDRDKSRLGF 448

Query: 184 SRRGCGLP 192
           +RRGC LP
Sbjct: 449 TRRGCALP 455

[Close]

BLAST of Cla002325 vs. TrEMBL
Match: V4KZL9_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10004188mg PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 9.5e-65
Identity = 125/190 (65.79%), Postives = 153/190 (80.53%), Query Frame = 1


Query: 3   GGGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGG 62
           GGG+RS  V+   ++S+TPL  NPLSPTFYY+ + SI V+G KL I+P+VW ID+ GNGG
Sbjct: 269 GGGVRSDAVS---KLSFTPLLTNPLSPTFYYVRLKSIFVNGAKLRIDPSVWEIDDSGNGG 328

Query: 63  TVVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESR-RPSLPRLR 122
           TVVDSGTTL +LA+ AY  V+ AVR+R++LP AAE+TPGFDLCVN SG S+    +PRL+
Sbjct: 329 TVVDSGTTLAFLAEPAYRSVIAAVRRRIRLPIAAEVTPGFDLCVNISGVSKPEKIMPRLK 388

Query: 123 LELKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRL 182
            EL GGA+F PPPRNYF+ETEE++ CLAI++V    GFSVIGNLMQQGFL EFDR+ SRL
Sbjct: 389 FELAGGALFVPPPRNYFIETEEQIQCLAIQSVNPKVGFSVIGNLMQQGFLFEFDRDRSRL 448

Query: 183 GFSRRGCGLP 192
           GFSRRGC LP
Sbjct: 449 GFSRRGCALP 455

[Close]

BLAST of Cla002325 vs. TrEMBL
Match: F6HF17_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g02930 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 6.2e-64
Identity = 117/176 (66.48%), Postives = 148/176 (84.09%), Query Frame = 1


Query: 16  RISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYLA 75
           R+ +TPL INPLSPTFYYIGI S++VDG+KLPINP+VWA+DE GNGGT+VDSGTTLT+L 
Sbjct: 284 RMRFTPLHINPLSPTFYYIGIESVSVDGIKLPINPSVWALDELGNGGTIVDSGTTLTFLP 343

Query: 76  KAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPR 135
           + AY ++L  +++RV+LP+ AE TPGFDLCVN S E   P LP+L  +L G +VF+PPPR
Sbjct: 344 EPAYLQILTVIKRRVRLPSPAEPTPGFDLCVNVS-EIEHPRLPKLSFKLGGDSVFSPPPR 403

Query: 136 NYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGCGLP 192
           NYF++T+E V CLA++AV + +GFSVIGNLMQQGFLLEFD++ +RLGFSR GC LP
Sbjct: 404 NYFVDTDEDVKCLALQAVMTPSGFSVIGNLMQQGFLLEFDKDRTRLGFSRHGCALP 458

[Close]

BLAST of Cla002325 vs. TAIR10
Match: AT3G25700.1 (AT3G25700.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 245.4 bits (625), Expect = 2.9e-65
Identity = 117/178 (65.73%), Postives = 141/178 (79.21%), Query Frame = 1


Query: 15  TRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYL 74
           +++ +TPL  NPLSPTFYY+ + S+ V+G KL I+P++W ID+ GNGGTVVDSGTTL +L
Sbjct: 275 SKLFFTPLLTNPLSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFL 334

Query: 75  AKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESR-RPSLPRLRLELKGGAVFAPP 134
           A+ AY  V+ AVR+RVKLP A  LTPGFDLCVN SG ++    LPRL+ E  GGAVF PP
Sbjct: 335 AEPAYRSVIAAVRRRVKLPIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPP 394

Query: 135 PRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGCGLP 192
           PRNYF+ETEE++ CLAI++V+   GFSVIGNLMQQGFL EFDR+ SRLGFSRRGC LP
Sbjct: 395 PRNYFIETEEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

[Close]

BLAST of Cla002325 vs. TAIR10
Match: AT2G42980.1 (AT2G42980.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 107.8 bits (268), Expect = 7.3e-24
Identity = 66/162 (40.74%), Postives = 84/162 (51.85%), Query Frame = 1


Query: 30  TFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYLAKAAYEEVLKAVRQR 89
           TFYYI I SI V G  L I    W I   G+GGT++DSGTTL+Y A+ AYE +     ++
Sbjct: 365 TFYYIQIKSILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEK 424

Query: 90  VK--LPNAAELTPGFDLCVNASG-ESRRPSLPRLRLELKGGAVFAPPPRNYFLETEERVM 149
           +K   P   +  P  D C N SG E     LP L +    G V+  P  N F+   E ++
Sbjct: 425 MKENYPIFRDF-PVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLV 484

Query: 150 CLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGC 189
           CLAI        FS+IGN  QQ F + +D + SRLGF+   C
Sbjct: 485 CLAILGTPKST-FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

[Close]

BLAST of Cla002325 vs. TAIR10
Match: AT3G59080.1 (AT3G59080.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 104.4 bits (259), Expect = 8.1e-23
Identity = 64/164 (39.02%), Postives = 85/164 (51.83%), Query Frame = 1


Query: 27  LSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYLAKAAYEEVLKAV 86
           L  TFYY+ I SI V G  L I    W I   G GGT++DSGTTL+Y A+ AYE +   +
Sbjct: 372 LVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKI 431

Query: 87  RQRV--KLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNYFLETEER 146
            ++   K P   +  P  D C N SG      LP L +    GAV+  P  N F+   E 
Sbjct: 432 AEKAKGKYPVYRDF-PILDPCFNVSG-IHNVQLPELGIAFADGAVWNFPTENSFIWLNED 491

Query: 147 VMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGC 189
           ++CLA+      + FS+IGN  QQ F + +D + SRLG++   C
Sbjct: 492 LVCLAMLGTPK-SAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

[Close]

BLAST of Cla002325 vs. TAIR10
Match: AT2G03200.1 (AT2G03200.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 100.5 bits (249), Expect = 1.2e-21
Identity = 52/165 (31.52%), Postives = 83/165 (50.30%), Query Frame = 1


Query: 25  NPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGTVVDSGTTLTYLAKAAYEEVLK 84
           NP  P+FYY+ +  ITV   +L +  + + + E G GG ++DSGTT+TYL + A++ + +
Sbjct: 298 NPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKE 357

Query: 85  AVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNYFLETEER 144
               R+ LP     + G DLC      ++  ++P++    KG  +  P       ++   
Sbjct: 358 EFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTG 417

Query: 145 VMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGCG 190
           V+CL   A+ S NG S+ GN+ QQ F +  D E   + F    CG
Sbjct: 418 VLCL---AMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECG 459

[Close]

BLAST of Cla002325 vs. TAIR10
Match: AT1G01300.1 (AT1G01300.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 99.8 bits (247), Expect = 2.0e-21
Identity = 60/172 (34.88%), Postives = 91/172 (52.91%), Query Frame = 1


Query: 19  YTPLQINPLSPTFYYIGIHSITVDGVKLP-INPAVWAIDEQGNGGTVVDSGTTLTYLAKA 78
           +TPL  NP   TFYY+G+  I+V G ++P +  +++ +D+ GNGG ++DSGT++T L + 
Sbjct: 317 FTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRP 376

Query: 79  AYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLELKGGAVFAPPPRNY 138
           AY  +  A R   K    A     FD C + S       +P + L  +G  V + P  NY
Sbjct: 377 AYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLS-NMNEVKVPTVVLHFRGADV-SLPATNY 436

Query: 139 FLETEER-VMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGFSRRGC 189
            +  +     C A     +  G S+IGN+ QQGF + +D  +SR+GF+  GC
Sbjct: 437 LIPVDTNGKFCFAFAG--TMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Cla002325 vs. NCBI nr
Match: gi|659073000|ref|XP_008467208.1| (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo])

HSP 1 Score: 354.4 bits (908), Expect = 1.3e-94
Identity = 174/191 (91.10%), Postives = 181/191 (94.76%), Query Frame = 1


Query: 1   MIGGGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGN 60
           MIGGGL SLPVNNAT+ISYTPLQINPLSPTFYYI I+SIT+DGVKLPINPAVW IDEQGN
Sbjct: 269 MIGGGLHSLPVNNATKISYTPLQINPLSPTFYYITINSITIDGVKLPINPAVWEIDEQGN 328

Query: 61  GGTVVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRL 120
           GGTVVDSGTTLTYL K AYEEVLK+VR+RVKLPNAAELTPGFDLCVNASGESRRPSLPRL
Sbjct: 329 GGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRL 388

Query: 121 RLELKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASR 180
           R  L GGAVFAPPPRNYFLETEE VMCLAIRAVESGNGFSVIGNLMQQGFLLEFD+E SR
Sbjct: 389 RFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESR 448

Query: 181 LGFSRRGCGLP 192
           LGF+RRGCGLP
Sbjct: 449 LGFTRRGCGLP 459

[Close]

BLAST of Cla002325 vs. NCBI nr
Match: gi|449451908|ref|XP_004143702.1| (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis sativus])

HSP 1 Score: 353.6 bits (906), Expect = 2.2e-94
Identity = 173/191 (90.58%), Postives = 180/191 (94.24%), Query Frame = 1


Query: 1   MIGGGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGN 60
           MIGGGL SLP+ NAT+ISYTPLQINPLSPTFYYI IHSIT+DGVKLPINPAVW IDEQGN
Sbjct: 269 MIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIHSITIDGVKLPINPAVWEIDEQGN 328

Query: 61  GGTVVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRL 120
           GGTVVDSGTTLTYL K AYEEVLK+VR+RVKLPNAAELTPGFDLCVNASGESRRPSLPRL
Sbjct: 329 GGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAELTPGFDLCVNASGESRRPSLPRL 388

Query: 121 RLELKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASR 180
           R  L GGAVFAPPPRNYFLETEE VMCLAIRAVESGNGFSVIGNLMQQGFLLEFD+E SR
Sbjct: 389 RFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDKEESR 448

Query: 181 LGFSRRGCGLP 192
           LGF+RRGCGLP
Sbjct: 449 LGFTRRGCGLP 459

[Close]

BLAST of Cla002325 vs. NCBI nr
Match: gi|802680767|ref|XP_012082020.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Jatropha curcas])

HSP 1 Score: 264.2 bits (674), Expect = 1.7e-67
Identity = 124/188 (65.96%), Postives = 154/188 (81.91%), Query Frame = 1


Query: 4   GGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGT 63
           GG ++  V+    +++TPL +N LSPTFYYIGI S++VDGVKLPINP+VW+ID+ GNGGT
Sbjct: 269 GGHQNSAVSRKRILNFTPLLVNSLSPTFYYIGIKSVSVDGVKLPINPSVWSIDDLGNGGT 328

Query: 64  VVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLE 123
           ++DSGTTLT+L + AY E+L A+++RVKLP   ELTPGFDLCVN SG  RRP  PR+ LE
Sbjct: 329 IIDSGTTLTFLVEPAYREILSAIKRRVKLPGPGELTPGFDLCVNVSG-VRRPVFPRMSLE 388

Query: 124 LKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGF 183
           L G +VF+PPPRNYF++T E V CLAI+ V SG+GFSVIGNLMQQG+LLEFDR+ SRLGF
Sbjct: 389 LAGNSVFSPPPRNYFIDTSEGVKCLAIQPVNSGSGFSVIGNLMQQGYLLEFDRDRSRLGF 448

Query: 184 SRRGCGLP 192
           +R GC LP
Sbjct: 449 ARSGCALP 455

[Close]

BLAST of Cla002325 vs. NCBI nr
Match: gi|255566835|ref|XP_002524401.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Ricinus communis])

HSP 1 Score: 258.1 bits (658), Expect = 1.2e-65
Identity = 119/188 (63.30%), Postives = 154/188 (81.91%), Query Frame = 1


Query: 4   GGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGGT 63
           GG +++ V+    +S+TPL INPLSPTFYYI I  + V+GVKLPINP+VW+ID+ GNGGT
Sbjct: 269 GGAQNVAVSKKGIMSFTPLLINPLSPTFYYIAIKGVYVNGVKLPINPSVWSIDDLGNGGT 328

Query: 64  VVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESRRPSLPRLRLE 123
           ++DSGTTLT++ + AY E+LKA ++RVKLP+ AE TPGFDLC+N SG + RP+LPR+   
Sbjct: 329 IIDSGTTLTFITEPAYTEILKAFKKRVKLPSPAEPTPGFDLCMNVSGVT-RPALPRMSFN 388

Query: 124 LKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRLGF 183
           L GG+VF+PPPRNYF+ET +++ CLA++ V    GFSV+GNLMQQGFLLEFDR+ SRLGF
Sbjct: 389 LAGGSVFSPPPRNYFIETGDQIKCLAVQPVSQDGGFSVLGNLMQQGFLLEFDRDKSRLGF 448

Query: 184 SRRGCGLP 192
           +RRGC LP
Sbjct: 449 TRRGCALP 455

[Close]

BLAST of Cla002325 vs. NCBI nr
Match: gi|567142517|ref|XP_006395632.1| (hypothetical protein EUTSA_v10004188mg [Eutrema salsugineum])

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-64
Identity = 125/190 (65.79%), Postives = 153/190 (80.53%), Query Frame = 1


Query: 3   GGGLRSLPVNNATRISYTPLQINPLSPTFYYIGIHSITVDGVKLPINPAVWAIDEQGNGG 62
           GGG+RS  V+   ++S+TPL  NPLSPTFYY+ + SI V+G KL I+P+VW ID+ GNGG
Sbjct: 269 GGGVRSDAVS---KLSFTPLLTNPLSPTFYYVRLKSIFVNGAKLRIDPSVWEIDDSGNGG 328

Query: 63  TVVDSGTTLTYLAKAAYEEVLKAVRQRVKLPNAAELTPGFDLCVNASGESR-RPSLPRLR 122
           TVVDSGTTL +LA+ AY  V+ AVR+R++LP AAE+TPGFDLCVN SG S+    +PRL+
Sbjct: 329 TVVDSGTTLAFLAEPAYRSVIAAVRRRIRLPIAAEVTPGFDLCVNISGVSKPEKIMPRLK 388

Query: 123 LELKGGAVFAPPPRNYFLETEERVMCLAIRAVESGNGFSVIGNLMQQGFLLEFDREASRL 182
            EL GGA+F PPPRNYF+ETEE++ CLAI++V    GFSVIGNLMQQGFL EFDR+ SRL
Sbjct: 389 FELAGGALFVPPPRNYFIETEEQIQCLAIQSVNPKVGFSVIGNLMQQGFLFEFDRDRSRL 448

Query: 183 GFSRRGCGLP 192
           GFSRRGC LP
Sbjct: 449 GFSRRGCALP 455

The following BLAST results are available for this feature:

BLAST of Cla002325 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NEP1_NEPGR	7.6e-23	34.57	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1	[more]
APF2_ARATH	3.5e-20	34.88	Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1	[more]
NEP2_NEPGR	6.0e-20	32.94	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1	[more]
ASPG2_ARATH	1.0e-19	33.87	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 ...	[more]
ASPG1_ARATH	5.8e-15	30.00	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 ...	[more]

BLAST of Cla002325 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNH6_CUCSA	1.5e-94	90.58	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G174650 PE=3 SV=1	[more]
A0A067K2U7_JATCU	1.2e-67	65.96	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18279 PE=3 SV=1	[more]
B9SEI2_RICCO	8.6e-66	63.30	Basic 7S globulin 2 small subunit, putative OS=Ricinus communis GN=RCOM_0705030 ...	[more]
V4KZL9_EUTSA	9.5e-65	65.79	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10004188mg PE=3 SV=1	[more]
F6HF17_VITVI	6.2e-64	66.48	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g02930 PE=3 SV=...	[more]

BLAST of Cla002325 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G25700.1	2.9e-65	65.73	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	7.3e-24	40.74	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	8.1e-23	39.02	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	1.2e-21	31.52	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	2.0e-21	34.88	Eukaryotic aspartyl protease family protein	[more]

BLAST of Cla002325 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659073000\|ref\|XP_008467208.1\|	1.3e-94	91.10	PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo]	[more]
gi\|449451908\|ref\|XP_004143702.1\|	2.2e-94	90.58	PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis sativus]	[more]
gi\|802680767\|ref\|XP_012082020.1\|	1.7e-67	65.96	PREDICTED: aspartic proteinase nepenthesin-1 [Jatropha curcas]	[more]
gi\|255566835\|ref\|XP_002524401.1\|	1.2e-65	63.30	PREDICTED: aspartic proteinase nepenthesin-1 [Ricinus communis]	[more]
gi\|567142517\|ref\|XP_006395632.1\|	1.4e-64	65.79	hypothetical protein EUTSA_v10004188mg [Eutrema salsugineum]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001461	Aspartic_peptidase_A1
IPR021109	Peptidase_aspartic_dom_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0030163	protein catabolic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0008233	peptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla002325	Cla002325.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 1..189 score: 2.1E
IPR021109	Aspartic peptidase domain	GENE3D	G3DSA:2.40.70.10		coord: 12..188 score: 3.0
IPR021109	Aspartic peptidase domain	unknown	SSF50630	Acid proteases	coord: 4..188 score: 6.07
None	No IPR available	PANTHER	PTHR13683:SF354	ASPARTYL PROTEASE FAMILY PROTEIN	coord: 1..189 score: 2.1E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla002325	Cla97C01G005980	Watermelon (97103) v2	wmwmbB238
Cla002325	Csa5G174650	Cucumber (Chinese Long) v2	cuwmB411
Cla002325	MELO3C005359	Melon (DHL92) v3.5.1	mewmB031
Cla002325	CSPI05G08550	Wild cucumber (PI 183967)	cpiwmB419
Cla002325	Cucsa.166240	Cucumber (Gy14) v1	cgywmB308
Cla002325	CmaCh15G014150	Cucurbita maxima (Rimu)	cmawmB274
Cla002325	CmoCh15G014930	Cucurbita moschata (Rifu)	cmowmB264
Cla002325	Lsi09G006010	Bottle gourd (USVL1VR-Ls)	lsiwmB030
Cla002325	Cp4.1LG13g00200	Cucurbita pepo (Zucchini)	cpewmB189
Cla002325	CsGy5G005930	Cucumber (Gy14) v2	cgybwmB384
Cla002325	MELO3C005359.2	Melon (DHL92) v3.6.1	medwmB030
Cla002325	Carg20763	Silver-seed gourd	carwmB0737
Cla002325	CsaV3_5G005980	Cucumber (Chinese Long) v3	cucwmB436
Cla002325	Bhi12G000705	Wax gourd	wgowmB080

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla002325	Watermelon (Charleston Gray)	wcgwmB145
Cla002325	Watermelon (Charleston Gray)	wcgwmB297
Cla002325	Cucurbita pepo (Zucchini)	cpewmB186
Cla002325	Cucurbita pepo (Zucchini)	cpewmB710
Cla002325	Melon (DHL92) v3.6.1	medwmB064
Cla002325	Silver-seed gourd	carwmB0710
Cla002325	Silver-seed gourd	carwmB0875
Cla002325	Cucumber (Chinese Long) v3	cucwmB446
Cla002325	Watermelon (97103) v2	wmwmbB267
Cla002325	Wax gourd	wgowmB556
Cla002325	Watermelon (97103) v1	wmwmB124
Cla002325	Cucurbita maxima (Rimu)	cmawmB280
Cla002325	Cucurbita maxima (Rimu)	cmawmB322
Cla002325	Cucurbita moschata (Rifu)	cmowmB270