Cla001442 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla001442
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Cysteine proteinase inhibitor 8 (AHRD V1 *--- CYT8_ORYSJ); contains Interpro domain(s) IPR009994 Phloem filament PP1
Location	Chr9 : 23308055 .. 23308657 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTGTGAGCCAAACGATGCGTGGGTCCCAATCCCTGATGCAGGAACGCCTTGTGTGAAAGATATTGGAAACTTTTCATGTAACGAGCACAACAAACAAACTGGAGATAATCTGACACTGAAGAGCATTATATCAGGATCGTTTAGGGAGATTAGTGACTATCAAATAGAGCATAAGTTGTATATTGAAGCAACAGATGTTAATGGAGTTGTAGCTACATATGAACCACATGTATATGTTGAAGAGAAAAATGGGATAAGAGAGAGGACACTGATTTCATTCCCACAAGGGTACAAGGATGAAAATGGTCAATTCTGGATTAAAATTCCTGACCTTGAGGAGAGTTGTGTTCAACATGCTTCAAAGTTTGCAACGAACACACATAACAAGGATGCTGATGATAATCTCATATATGTTTCAACTGTTGATGGTTATTATTATGAGGTGAATCCTTATGCTATAAGGTTCGATCTACGTATTAAGGCCAAGGATTGCTTTGGACGTTGGCGAATCTACAGGGCTATTGTGCTTGAAGACAAACCTCTTACTGAGAAAATTCGAGTACTCGAATATTTCAAAGTTATCCCAAGGTTACGTTGA

mRNA sequence

ATGAGTTGTGAGCCAAACGATGCGTGGGTCCCAATCCCTGATGCAGGAACGCCTTGTGTGAAAGATATTGGAAACTTTTCATGTAACGAGCACAACAAACAAACTGGAGATAATCTGACACTGAAGAGCATTATATCAGGATCGTTTAGGGAGATTAGTGACTATCAAATAGAGCATAAGTTGTATATTGAAGCAACAGATGTTAATGGAGTTGTAGCTACATATGAACCACATGTATATGTTGAAGAGAAAAATGGGATAAGAGAGAGGACACTGATTTCATTCCCACAAGGGTACAAGGATGAAAATGGTCAATTCTGGATTAAAATTCCTGACCTTGAGGAGAGTTGTGTTCAACATGCTTCAAAGTTTGCAACGAACACACATAACAAGGATGCTGATGATAATCTCATATATGTTTCAACTGTTGATGGTTATTATTATGAGGTGAATCCTTATGCTATAAGGTTCGATCTACGTATTAAGGCCAAGGATTGCTTTGGACGTTGGCGAATCTACAGGGCTATTGTGCTTGAAGACAAACCTCTTACTGAGAAAATTCGAGTACTCGAATATTTCAAAGTTATCCCAAGGTTACGTTGA

Coding sequence (CDS)

ATGAGTTGTGAGCCAAACGATGCGTGGGTCCCAATCCCTGATGCAGGAACGCCTTGTGTGAAAGATATTGGAAACTTTTCATGTAACGAGCACAACAAACAAACTGGAGATAATCTGACACTGAAGAGCATTATATCAGGATCGTTTAGGGAGATTAGTGACTATCAAATAGAGCATAAGTTGTATATTGAAGCAACAGATGTTAATGGAGTTGTAGCTACATATGAACCACATGTATATGTTGAAGAGAAAAATGGGATAAGAGAGAGGACACTGATTTCATTCCCACAAGGGTACAAGGATGAAAATGGTCAATTCTGGATTAAAATTCCTGACCTTGAGGAGAGTTGTGTTCAACATGCTTCAAAGTTTGCAACGAACACACATAACAAGGATGCTGATGATAATCTCATATATGTTTCAACTGTTGATGGTTATTATTATGAGGTGAATCCTTATGCTATAAGGTTCGATCTACGTATTAAGGCCAAGGATTGCTTTGGACGTTGGCGAATCTACAGGGCTATTGTGCTTGAAGACAAACCTCTTACTGAGAAAATTCGAGTACTCGAATATTTCAAAGTTATCCCAAGGTTACGTTGA

Protein sequence

MSCEPNDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEATDVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQFWIKIPDLEESCVQHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPLTEKIRVLEYFKVIPRLR

Homology

[Close]

BLAST of Cla001442 vs. TrEMBL
Match: A0A0A0K3W6_CUCSA (Phloem filament protein OS=Cucumis sativus GN=Csa_7G222880 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-49
Identity = 103/198 (52.02%), Postives = 131/198 (66.16%), Query Frame = 1


Query: 1   MSCEPNDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHK 60
           MSC+    W+ IPD  TPCV ++     N+HNK +GD+L  K +I G F EI D+ I+H+
Sbjct: 1   MSCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHR 60

Query: 61  LYIEATDVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQ--FWIKIPDLEESCV 120
           L IE     GVV  YE  + V +K+G R RTLISF  GY DE     FWIKIPD+++SCV
Sbjct: 61  LIIEVVTSKGVVLIYELIIVVVQKDGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSCV 120

Query: 121 QHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVL 180
           Q  +K+A   HNK   D L Y STV+G+Y+EVNP+ I F L IKAK+C GR R Y A+V 
Sbjct: 121 QDCAKWAVTEHNKITHDYLAYDSTVEGWYHEVNPFVIIFKLHIKAKECIGRVRDYIAVVK 180

Query: 181 EDKPLTEKIRVLEYFKVI 197
           E+KPLTEKI+ LE F +I
Sbjct: 181 EEKPLTEKIKKLEDFYLI 198

[Close]

BLAST of Cla001442 vs. TrEMBL
Match: A0A0A0LYF4_CUCSA (Phloem filament protein OS=Cucumis sativus GN=Csa_1G710160 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 5.5e-47
Identity = 101/200 (50.50%), Postives = 131/200 (65.50%), Query Frame = 1


Query: 1   MSCEPNDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHK 60
           MSC     W  IP   TPCV DI NF+  EHNK+TG+ LTLKS+I G F E+ D +++ +
Sbjct: 1   MSCVDKGEWSKIPVCTTPCVLDIANFAVTEHNKETGEKLTLKSVIKGWFLELGDVKLKFR 60

Query: 61  LYIEATDVNGVVATYEPHVYVEEKNGI-RERTLISFPQGYKDENGQFWIKIPDLEESCVQ 120
           LYI A +  GVV TYE  V V E     R + L+SF  GY DE   FWI I D + SCVQ
Sbjct: 61  LYILAANDKGVVLTYEAVVCVTEIGDFKRVKKLLSFNVGYLDEKDVFWIVIVDTKASCVQ 120

Query: 121 HASKFATNTHNKDADDNLIYVSTVDGYYYEVNPY-AIRFDLRIKAKDCFGRWRIYRAIVL 180
             +KFA   HN+D  D+L+Y+S   G+Y E++PY AI F++ +  KDCFGR R ++A+VL
Sbjct: 121 DVAKFAVAKHNEDEHDSLVYLSITKGWYRELDPYNAIFFEIHLTTKDCFGRVREFKALVL 180

Query: 181 EDKPLTEKIRVLEYFKVIPR 199
           EDKP  EKIR L+YF+VI +
Sbjct: 181 EDKPQKEKIRTLKYFEVIKK 200

[Close]

BLAST of Cla001442 vs. TrEMBL
Match: P94012_CUCMA (Phloem filament protein OS=Cucurbita maxima GN=PP1 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.9e-24
Identity = 75/199 (37.69%), Postives = 105/199 (52.76%), Query Frame = 1


Query: 7   DAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEAT 66
           + W+ IPD    CV+++   +  + N Q G +L   SI  G + E+    ++++L+I A 
Sbjct: 15  EKWIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHIRAI 74

Query: 67  DVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQF-----------WIKIPDLEE 126
           D       YE  V+ E+    R R LISF   Y    G +           WIKIPDL  
Sbjct: 75  DFLQRSLLYEALVFEEKPKLERIRKLISFL--YLLNGGHYVGPVDPPQVEKWIKIPDLRV 134

Query: 127 SCVQHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRA 186
             VQ  SKFA + +NK  +  L YV   DG+Y E+    I+F L +KAKDC GR R Y A
Sbjct: 135 PFVQEVSKFAIDEYNKGGE-GLKYVEIYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEA 194

Query: 187 IVLEDKPLTEKIRVLEYFK 195
           IVL  + L++KI++LE FK
Sbjct: 195 IVLVKQFLSKKIKILESFK 210

[Close]

BLAST of Cla001442 vs. TrEMBL
Match: P94012_CUCMA (Phloem filament protein OS=Cucurbita maxima GN=PP1 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-15
Identity = 57/195 (29.23%), Postives = 98/195 (50.26%), Query Frame = 1


Query: 9   WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEATDV 68
           W+ IP+   P  + + NF   +     GD L   SI  G + E+    ++ +L+I+A D 
Sbjct: 613 WIQIPNLKEPGFQVVINFIVEQLKIILGDCLKFHSIYEGWYFELCPISLKFRLHIKAIDF 672

Query: 69  NGVVATYEPHVYVEEKNGIRERTLISFPQGY-----KDENGQFWIKIPDLEESCVQHASK 128
            G    YE  +  E++    E  ++    G+          + WIKI +L+ + +Q  SK
Sbjct: 673 LGRCLNYEIIIIEEKRIFKLESIIVILSPGHCVGPVDPPKVEKWIKISNLQFAFLQEVSK 732

Query: 129 FATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPL 188
           FA +  N  + D+L +    DG+Y E+    I+F + ++AKDC  R   Y A V   + L
Sbjct: 733 FALDDFNVKSGDSLRFDGIYDGWYMEMGQDNIKFRIHLRAKDCLSRVHHYEAYVYVKQFL 792

Query: 189 TEKIRVLEYFKVIPR 199
           +++I+++E FK+I R
Sbjct: 793 SKRIKIVESFKLIER 807

HSP 2 Score: 90.5 bits (223), Expect = 2.5e-15
Identity = 60/186 (32.26%), Postives = 96/186 (51.61%), Query Frame = 1


Query: 1   MSCEPNDA-WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEH 60
           +S EP +  W+ IP+   P V+++  F+ +EHNK  GD L    +  G F ++    I+ 
Sbjct: 507 VSPEPQEKKWIKIPNVQVPLVQELAKFAVDEHNKN-GDGLKYIEVYEGWFMDLGHDNIKF 566

Query: 61  KLYIEATDVNGVVATYEPHVYVEEKNGIRERTLISF----PQGYKDENGQFWIKIPDLEE 120
           +L+++A D  G +  YE  V VE     R + L SF    P  ++ +    WI+IP+L+E
Sbjct: 567 RLHLKAKDWLGRIRNYEAVVLVEHFWSKRIKILESFKFCGPLVFEPK----WIQIPNLKE 626

Query: 121 SCVQHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRA 180
              Q    F          D L + S  +G+Y+E+ P +++F L IKA D  GR   Y  
Sbjct: 627 PGFQVVINFIVEQLKIILGDCLKFHSIYEGWYFELCPISLKFRLHIKAIDFLGRCLNYEI 686

Query: 181 IVLEDK 182
           I++E+K
Sbjct: 687 IIIEEK 687

HSP 3 Score: 85.5 bits (210), Expect = 8.0e-14
Identity = 54/188 (28.72%), Postives = 98/188 (52.13%), Query Frame = 1


Query: 9   WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEATDV 68
           W  IPD     ++ +  F   +   + GD+L  + +  G + E+    ++++ +++  D 
Sbjct: 221 WNQIPDVKELGLQVVIKFIVEQIKIKFGDSLKFEIVYEGWYFELCPNSLKYRFHVKVIDF 280

Query: 69  NGVVATYEPHVYVEEKNGIRERTLISFPQGYKDE-NGQFWIKIPDLEESCVQHASKFATN 128
            G    +E  +  E +  +R   L S       E   + WIKIP+++   VQ  +KFA +
Sbjct: 281 LGRSLKFEIIIIEERRVLLRIWKLDSIIVIVSPEPQEKKWIKIPNVQVPLVQELAKFAVD 340

Query: 129 THNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPLTEKI 188
            HNK+ D  L Y+   +G++ ++    I+F L +KAKD  GR R Y A+VL +   +++I
Sbjct: 341 EHNKNGD-GLKYIEVYEGWFMDLGHDNIKFRLHLKAKDWLGRIRNYEAVVLVEHFWSKRI 400

Query: 189 RVLEYFKV 196
           ++LE FK+
Sbjct: 401 KILESFKL 407

HSP 4 Score: 84.3 bits (207), Expect = 1.8e-13
Identity = 52/187 (27.81%), Postives = 98/187 (52.41%), Query Frame = 1


Query: 9   WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEATDV 68
           W+ IP+     ++ +  F   +   + GD+L  + +  G +  +    ++++ +++  D 
Sbjct: 417 WIQIPNLKELGLQVVIKFIVEQIKIKFGDSLKFEIVYEGWYFGLCPNSLKYRFHVKVIDF 476

Query: 69  NGVVATYEPHVYVEEKNGIRERTLISFPQGYKDE-NGQFWIKIPDLEESCVQHASKFATN 128
            G    +E  +  E ++ +R   L S       E   + WIKIP+++   VQ  +KFA +
Sbjct: 477 LGRPLKFEVVIIEEIRDHLRIWKLDSIIAIVSPEPQEKKWIKIPNVQVPLVQELAKFAVD 536

Query: 129 THNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPLTEKI 188
            HNK+ D  L Y+   +G++ ++    I+F L +KAKD  GR R Y A+VL +   +++I
Sbjct: 537 EHNKNGD-GLKYIEVYEGWFMDLGHDNIKFRLHLKAKDWLGRIRNYEAVVLVEHFWSKRI 596

Query: 189 RVLEYFK 195
           ++LE FK
Sbjct: 597 KILESFK 602

HSP 5 Score: 84.0 bits (206), Expect = 2.3e-13
Identity = 51/190 (26.84%), Postives = 97/190 (51.05%), Query Frame = 1


Query: 7   DAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEAT 66
           + W+ IPD   P V+++  F+ +E+NK  G+ L    I  G + E+    I+ +L+++A 
Sbjct: 122 EKWIKIPDLRVPFVQEVSKFAIDEYNK-GGEGLKYVEIYDGWYAEMGQDNIKFRLHVKAK 181

Query: 67  DVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQFWIKIPDLEESCVQHASKFAT 126
           D  G +  YE  V V++    + + L SF    + +    W +IPD++E  +Q   KF  
Sbjct: 182 DCLGRLRNYEAIVLVKQFLSKKIKILESFKFCDQGKVSPGWNQIPDVKELGLQVVIKFIV 241

Query: 127 NTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPLTEK 186
                   D+L +    +G+Y+E+ P ++++   +K  D  GR   +  I++E++ +  +
Sbjct: 242 EQIKIKFGDSLKFEIVYEGWYFELCPNSLKYRFHVKVIDFLGRSLKFEIIIIEERRVLLR 301

Query: 187 IRVLEYFKVI 197
           I  L+   VI
Sbjct: 302 IWKLDSIIVI 310

HSP 6 Score: 91.3 bits (225), Expect = 1.5e-15
Identity = 57/195 (29.23%), Postives = 98/195 (50.26%), Query Frame = 1


Query: 9   WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEATDV 68
           W+ IP+   P  + + NF   +     GD L   SI  G + E+    ++ +L+I+A D 
Sbjct: 224 WIQIPNLKEPGFQVVINFIVEQLKIILGDCLKFHSIYEGWYFELCPISLKFRLHIKAIDF 283

Query: 69  NGVVATYEPHVYVEEKNGIRERTLISFPQGY-----KDENGQFWIKIPDLEESCVQHASK 128
            G    YE  +  E++    E  ++    G+          + WIKI +L+ + +Q  SK
Sbjct: 284 LGRCLNYEIIIIEEKRIFKLESIIVILSPGHCVGPVDPPKVEKWIKISNLQFAFLQEVSK 343

Query: 129 FATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPL 188
           FA +  N  + D+L +    DG+Y E+    I+F + ++AKDC  R   Y A V   + L
Sbjct: 344 FALDDFNVKSGDSLRFDGIYDGWYMEMGQDNIKFRIHLRAKDCLSRVHHYEAYVYVKQFL 403

Query: 189 TEKIRVLEYFKVIPR 199
           +++I+++E FK+I R
Sbjct: 404 SKRIKIVESFKLIER 418

[Close]

BLAST of Cla001442 vs. TrEMBL
Match: Q7DMD5_CUCMA (Phloem filament protein (Fragment) OS=Cucurbita maxima GN=cPC7 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.5e-15
Identity = 60/186 (32.26%), Postives = 96/186 (51.61%), Query Frame = 1


Query: 1   MSCEPNDA-WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEH 60
           +S EP +  W+ IP+   P V+++  F+ +EHNK  GD L    +  G F ++    I+ 
Sbjct: 118 VSPEPQEKKWIKIPNVQVPLVQELAKFAVDEHNKN-GDGLKYIEVYEGWFMDLGHDNIKF 177

Query: 61  KLYIEATDVNGVVATYEPHVYVEEKNGIRERTLISF----PQGYKDENGQFWIKIPDLEE 120
           +L+++A D  G +  YE  V VE     R + L SF    P  ++ +    WI+IP+L+E
Sbjct: 178 RLHLKAKDWLGRIRNYEAVVLVEHFWSKRIKILESFKFCGPLVFEPK----WIQIPNLKE 237

Query: 121 SCVQHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRA 180
              Q    F          D L + S  +G+Y+E+ P +++F L IKA D  GR   Y  
Sbjct: 238 PGFQVVINFIVEQLKIILGDCLKFHSIYEGWYFELCPISLKFRLHIKAIDFLGRCLNYEI 297

Query: 181 IVLEDK 182
           I++E+K
Sbjct: 298 IIIEEK 298

HSP 2 Score: 84.3 bits (207), Expect = 1.8e-13
Identity = 52/187 (27.81%), Postives = 98/187 (52.41%), Query Frame = 1


Query: 9   WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEATDV 68
           W+ IP+     ++ +  F   +   + GD+L  + +  G +  +    ++++ +++  D 
Sbjct: 28  WIQIPNLKELGLQVVIKFIVEQIKIKFGDSLKFEIVYEGWYFGLCPNSLKYRFHVKVIDF 87

Query: 69  NGVVATYEPHVYVEEKNGIRERTLISFPQGYKDE-NGQFWIKIPDLEESCVQHASKFATN 128
            G    +E  +  E ++ +R   L S       E   + WIKIP+++   VQ  +KFA +
Sbjct: 88  LGRPLKFEVVIIEEIRDHLRIWKLDSIIAIVSPEPQEKKWIKIPNVQVPLVQELAKFAVD 147

Query: 129 THNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPLTEKI 188
            HNK+ D  L Y+   +G++ ++    I+F L +KAKD  GR R Y A+VL +   +++I
Sbjct: 148 EHNKNGD-GLKYIEVYEGWFMDLGHDNIKFRLHLKAKDWLGRIRNYEAVVLVEHFWSKRI 207

Query: 189 RVLEYFK 195
           ++LE FK
Sbjct: 208 KILESFK 213

HSP 3 Score: 82.0 bits (201), Expect = 8.9e-13
Identity = 42/109 (38.53%), Postives = 63/109 (57.80%), Query Frame = 1


Query: 6   NDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEA 65
           ND W+ IPD  TPCV D+G ++ +++NK   DNL  K +I G + EI   + + +L IE 
Sbjct: 7   NDKWIQIPDTKTPCVIDLGKYAVDDYNKAHKDNLVFKKVIIGWYLEIDYDKTKLRLIIEV 66

Query: 66  TDVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQFWIKIPDLE 115
            +  G V  YE  + V++K+G + +T ISF  GY DE      ++ D E
Sbjct: 67  VNSKGEVIIYEVVIVVDDKDGKKVKTPISFIPGYIDEAKDCLGRVRDYE 115

[Close]

BLAST of Cla001442 vs. NCBI nr
Match: gi|700188975|gb|KGN44208.1| (Phloem filament protein [Cucumis sativus])

HSP 1 Score: 203.8 bits (517), Expect = 2.9e-49
Identity = 103/198 (52.02%), Postives = 131/198 (66.16%), Query Frame = 1


Query: 1   MSCEPNDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHK 60
           MSC+    W+ IPD  TPCV ++     N+HNK +GD+L  K +I G F EI D+ I+H+
Sbjct: 1   MSCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHR 60

Query: 61  LYIEATDVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQ--FWIKIPDLEESCV 120
           L IE     GVV  YE  + V +K+G R RTLISF  GY DE     FWIKIPD+++SCV
Sbjct: 61  LIIEVVTSKGVVLIYELIIVVVQKDGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSCV 120

Query: 121 QHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVL 180
           Q  +K+A   HNK   D L Y STV+G+Y+EVNP+ I F L IKAK+C GR R Y A+V 
Sbjct: 121 QDCAKWAVTEHNKITHDYLAYDSTVEGWYHEVNPFVIIFKLHIKAKECIGRVRDYIAVVK 180

Query: 181 EDKPLTEKIRVLEYFKVI 197
           E+KPLTEKI+ LE F +I
Sbjct: 181 EEKPLTEKIKKLEDFYLI 198

[Close]

BLAST of Cla001442 vs. NCBI nr
Match: gi|778730266|ref|XP_011659743.1| (PREDICTED: uncharacterized protein LOC101206992 [Cucumis sativus])

HSP 1 Score: 203.8 bits (517), Expect = 2.9e-49
Identity = 103/198 (52.02%), Postives = 131/198 (66.16%), Query Frame = 1


Query: 1   MSCEPNDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHK 60
           MSC+    W+ IPD  TPCV ++     N+HNK +GD+L  K +I G F EI D+ I+H+
Sbjct: 226 MSCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHR 285

Query: 61  LYIEATDVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQ--FWIKIPDLEESCV 120
           L IE     GVV  YE  + V +K+G R RTLISF  GY DE     FWIKIPD+++SCV
Sbjct: 286 LIIEVVTSKGVVLIYELIIVVVQKDGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSCV 345

Query: 121 QHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVL 180
           Q  +K+A   HNK   D L Y STV+G+Y+EVNP+ I F L IKAK+C GR R Y A+V 
Sbjct: 346 QDCAKWAVTEHNKITHDYLAYDSTVEGWYHEVNPFVIIFKLHIKAKECIGRVRDYIAVVK 405

Query: 181 EDKPLTEKIRVLEYFKVI 197
           E+KPLTEKI+ LE F +I
Sbjct: 406 EEKPLTEKIKKLEDFYLI 423

[Close]

BLAST of Cla001442 vs. NCBI nr
Match: gi|778730266|ref|XP_011659743.1| (PREDICTED: uncharacterized protein LOC101206992 [Cucumis sativus])

HSP 1 Score: 156.8 bits (395), Expect = 4.1e-35
Identity = 81/195 (41.54%), Postives = 120/195 (61.54%), Query Frame = 1


Query: 6   NDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEA 65
           ND W+ IPD  TPCV D+G ++ +++NK   DNL  K +I G + EI   + + +L IE 
Sbjct: 7   NDKWIQIPDTKTPCVIDLGKYAVDDYNKAHKDNLVFKKVIIGWYLEIDYDKTKLRLIIEV 66

Query: 66  TDVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQ--FWIKIPDLEESCVQHASK 125
            +  G V  YE  + V++K+G + +T ISF  GY DE     FW +I D+ +  VQ  +K
Sbjct: 67  VNSKGEVIIYEVVIVVDDKDGKKVKTPISFIPGYIDEVDPWLFWFRIIDINDPHVQQIAK 126

Query: 126 FATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRAIVLEDKPL 185
           FA   HN+D +D+L+Y+ST+  +Y  ++P   ++ + +KAKDC GR R Y A+V E+K L
Sbjct: 127 FAVEKHNQDNNDSLVYISTIVCWYRLLSPILKQYKVYLKAKDCLGRVRDYEALVEEEKKL 186

Query: 186 TEKIRVLEYFKVIPR 199
              I  L YFKVIP+
Sbjct: 187 PNAILKLIYFKVIPK 201

HSP 2 Score: 84.7 bits (208), Expect = 2.0e-13
Identity = 59/211 (27.96%), Postives = 96/211 (45.50%), Query Frame = 1


Query: 9   WVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEATDV 68
           W  I D   P V+ I  F+  +HN+   D+L   S I   +R +S    ++K+Y++A D 
Sbjct: 110 WFRIIDINDPHVQQIAKFAVEKHNQDNNDSLVYISTIVCWYRLLSPILKQYKVYLKAKDC 169

Query: 69  NGVVATYEPHVYVEEK-------------------NGIRERTLISFPQGYKDENGQF--- 128
            G V  YE  V  E+K                    G +  T  +F +  K  + +    
Sbjct: 170 LGRVRDYEALVEEEKKLPNAILKLIYFKVIPKKSEGGKKHITTKTFSKISKKRDSKMSCD 229

Query: 129 ----WIKIPDLEESCVQHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIK 188
               WIKIPD    CV   +K   N HNK + D+L+Y   + G+++E++ +AI+  L I+
Sbjct: 230 SKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHRLIIE 289

Query: 189 AKDCFGRWRIYRAIVLEDKPLTEKIRVLEYF 194
                G   IY  I++  +   +++R L  F
Sbjct: 290 VVTSKGVVLIYELIIVVVQKDGKRVRTLISF 320

HSP 3 Score: 195.7 bits (496), Expect = 7.9e-47
Identity = 101/200 (50.50%), Postives = 131/200 (65.50%), Query Frame = 1


Query: 1   MSCEPNDAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHK 60
           MSC     W  IP   TPCV DI NF+  EHNK+TG+ LTLKS+I G F E+ D +++ +
Sbjct: 1   MSCVDKGEWSKIPVCTTPCVLDIANFAVTEHNKETGEKLTLKSVIKGWFLELGDVKLKFR 60

Query: 61  LYIEATDVNGVVATYEPHVYVEEKNGI-RERTLISFPQGYKDENGQFWIKIPDLEESCVQ 120
           LYI A +  GVV TYE  V V E     R + L+SF  GY DE   FWI I D + SCVQ
Sbjct: 61  LYILAANDKGVVLTYEAVVCVTEIGDFKRVKKLLSFNVGYLDEKDVFWIVIVDTKASCVQ 120

Query: 121 HASKFATNTHNKDADDNLIYVSTVDGYYYEVNPY-AIRFDLRIKAKDCFGRWRIYRAIVL 180
             +KFA   HN+D  D+L+Y+S   G+Y E++PY AI F++ +  KDCFGR R ++A+VL
Sbjct: 121 DVAKFAVAKHNEDEHDSLVYLSITKGWYRELDPYNAIFFEIHLTTKDCFGRVREFKALVL 180

Query: 181 EDKPLTEKIRVLEYFKVIPR 199
           EDKP  EKIR L+YF+VI +
Sbjct: 181 EDKPQKEKIRTLKYFEVIKK 200

[Close]

BLAST of Cla001442 vs. NCBI nr
Match: gi|659092737|ref|XP_008447193.1| (PREDICTED: uncharacterized protein LOC103489701 [Cucumis melo])

HSP 1 Score: 155.6 bits (392), Expect = 9.1e-35
Identity = 84/203 (41.38%), Postives = 122/203 (60.10%), Query Frame = 1


Query: 1   MSCEPN-DAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEH 60
           MSCE   + W+ I D  TPCV + G  + ++HNK+ GDNL  K +I G + EI   + + 
Sbjct: 1   MSCETKKEQWIQIHDTTTPCVINFGKHAVDDHNKEKGDNLVFKKVIIGWYLEIDYNKTKL 60

Query: 61  KLYIEATDVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENG--QFWIKIPDLEESC 120
           +L IE  +  G V T+E  + V++K+G   +T+ISF  G+ DE     FWIKI ++ E  
Sbjct: 61  RLIIEVVNSKGEVITFEVVIVVDDKDGKTVKTVISFVPGHIDEKNPWTFWIKIHNVTEPH 120

Query: 121 VQHASKFATNTHNKDADDNLIYVSTVDGYYY-EVNPYAIRFDLRIKAKDCFGRWRIYRAI 180
           VQ  +KFA   HNK   DNLIY+  ++G+Y+    P    + L +KAKDC GR R Y A+
Sbjct: 121 VQEIAKFAVEEHNKITHDNLIYICIIEGWYHVSTIPPVTLWKLHLKAKDCLGRVREYEAL 180

Query: 181 VLEDKPLTEKIRVLEYFKVIPRL 200
           V E+K L + +  LE+F VIP++
Sbjct: 181 VKEEKLLPKHVLKLEHFIVIPKI 203

[Close]

BLAST of Cla001442 vs. NCBI nr
Match: gi|1753099|gb|AAC12676.1| (phloem filament protein [Cucurbita maxima])

HSP 1 Score: 120.2 bits (300), Expect = 4.2e-24
Identity = 75/199 (37.69%), Postives = 105/199 (52.76%), Query Frame = 1


Query: 7   DAWVPIPDAGTPCVKDIGNFSCNEHNKQTGDNLTLKSIISGSFREISDYQIEHKLYIEAT 66
           + W+ IPD    CV+++   +  + N Q G +L   SI  G + E+    ++++L+I A 
Sbjct: 15  EKWIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHIRAI 74

Query: 67  DVNGVVATYEPHVYVEEKNGIRERTLISFPQGYKDENGQF-----------WIKIPDLEE 126
           D       YE  V+ E+    R R LISF   Y    G +           WIKIPDL  
Sbjct: 75  DFLQRSLLYEALVFEEKPKLERIRKLISFL--YLLNGGHYVGPVDPPQVEKWIKIPDLRV 134

Query: 127 SCVQHASKFATNTHNKDADDNLIYVSTVDGYYYEVNPYAIRFDLRIKAKDCFGRWRIYRA 186
             VQ  SKFA + +NK  +  L YV   DG+Y E+    I+F L +KAKDC GR R Y A
Sbjct: 135 PFVQEVSKFAIDEYNKGGE-GLKYVEIYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEA 194

Query: 187 IVLEDKPLTEKIRVLEYFK 195
           IVL  + L++KI++LE FK
Sbjct: 195 IVLVKQFLSKKIKILESFK 210

The following BLAST results are available for this feature:

BLAST of Cla001442 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3W6_CUCSA	2.0e-49	52.02	Phloem filament protein OS=Cucumis sativus GN=Csa_7G222880 PE=4 SV=1	[more]
A0A0A0LYF4_CUCSA	5.5e-47	50.50	Phloem filament protein OS=Cucumis sativus GN=Csa_1G710160 PE=4 SV=1	[more]
P94012_CUCMA	2.9e-24	37.69	Phloem filament protein OS=Cucurbita maxima GN=PP1 PE=4 SV=1	[more]
P94012_CUCMA	1.5e-15	29.23	Phloem filament protein OS=Cucurbita maxima GN=PP1 PE=4 SV=1	[more]
Q7DMD5_CUCMA	2.5e-15	32.26	Phloem filament protein (Fragment) OS=Cucurbita maxima GN=cPC7 PE=2 SV=1	[more]

BLAST of Cla001442 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700188975\|gb\|KGN44208.1\|	2.9e-49	52.02	Phloem filament protein [Cucumis sativus]	[more]
gi\|778730266\|ref\|XP_011659743.1\|	2.9e-49	52.02	PREDICTED: uncharacterized protein LOC101206992 [Cucumis sativus]	[more]
gi\|778730266\|ref\|XP_011659743.1\|	4.1e-35	41.54	PREDICTED: uncharacterized protein LOC101206992 [Cucumis sativus]	[more]
gi\|659092737\|ref\|XP_008447193.1\|	9.1e-35	41.38	PREDICTED: uncharacterized protein LOC103489701 [Cucumis melo]	[more]
gi\|1753099\|gb\|AAC12676.1\|	4.2e-24	37.69	phloem filament protein [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR009994	PP1
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU38567	watermelon unigene v2 vs TrEMBL	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla001442	Cla001442.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU38567	WMU38567	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009994	Phloem filament PP1	PFAM	PF07430	PP1	coord: 117..193 score: 2.1
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PRODOM	PD001231		coord: 20..96 score: 3.0E-4coord: 122..194 score: 2.
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 16..95 score: 9.0
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 9..83 score: 2.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 107..194 score: 2.67E-7coord: 9..95 score: 8.5

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla001442	Cucurbita moschata (Rifu)	cmowmB074
Cla001442	Watermelon (Charleston Gray)	wcgwmB010
Cla001442	Watermelon (97103) v2	wmwmbB044