Cla001301 (gene) Watermelon (97103) v1

Overview
NameCla001301
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionHydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (AHRD V1 **-* D1GJ93_COFAR); contains Interpro domain(s) IPR003480 Transferase
LocationChr10 : 15406178 .. 15407288 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGTCATGTTCGTGGAGGCAAAGGCCCAGTGTCCGATGTCGGAGGTTATGAACGACAACAATTTACAACGCGAAGAGATGTTGAAGTTGCTTCTTTTAGATGACATGAATAAAAAGGACCAAGGATTATTGCATCCTTTGTTGTCTATTCAGCTGACTCAATTTGAATGTGGAGGAGAAGTGATTTGTGTGTTTTTTCATCACAAGCTTGCGGATATAGCCTCCATAACCAGCTTTATGAGAGATTGGGCTTCCATTGCTCGTAGCTTCGGTGACACACATCAACCGACGGTGAATCCTCAGTTCAATGGTGCTGACTTTTTCCGACCCGAATTCGACACAAACAACGGCCCAACTGGTGGCCAGAATGATTTAGGAATGAATGAAAAAGTTTGTACTAAGAGATTGGTGTTTGAAGGTTCGAAGATAGCAGCTCTTAAAGCTATGGTTCCTGGGAAAGTAGAGAATCCAACTCGTGTTCAGCTTATTGCTGCGTTCATCTACAAAGCTGCGCTTTCTGCAAAATTTTCAGTCACAGGTACGATCAATTTTAGTCACTGAATTGTAGTCAGAGAGTAAAAAAGTATATATTGAAACTTAGTTACAAAATGCACATACATTTATAAAATCACGAGGACTAAAACGGTAATTTCCTCTTGAATAAAAGTGTAGTTAACTAAAATAGTTGGGAAAGCAAGTAATGGTTTTATTGTTTTTGTTGTACAAAATAGGTAGTTTGCCAGAAACAAGTGCACTACTTCTTATGATCAACTTACGCAACAGAGTGGAGCCACCATTACCAACATTAATAGGTAACATAATCTCATTCTTCATAGCAACATCGACCACTAAGGAGCAAAGGGACATGGAGTTATGGGACATAGTGGTTGACATGAAGAGAAACTTACAAGAGTTTTGTAAAAAGTTCCCAAAAGATTACAAAGCTGAAGAATGGAGGCGTCTTTACAAATTACATGCCAAAGAATCAATGGAGAAATTGAGAAATGTGAAGGATTCTCCTAATGTGTATGCATGTACAAGTTGGTGCAAATTTCCAGTTTATGATGCTGATTTTGGATGGGGGAAGCCTATGGGTAACTGTACCTGA

mRNA sequence

ATGGGCGTCATGTTCGTGGAGGCAAAGGCCCAGTGTCCGATGTCGGAGGTTATGAACGACAACAATTTACAACGCGAAGAGATGTTGAAGTTGCTTCTTTTAGATGACATGAATAAAAAGGACCAAGGATTATTGCATCCTTTGTTGTCTATTCAGCTGACTCAATTTGAATGTGGAGGAGAAGTGATTTGTGTGTTTTTTCATCACAAGCTTGCGGATATAGCCTCCATAACCAGCTTTATGAGAGATTGGGCTTCCATTGCTCGTAGCTTCGGTGACACACATCAACCGACGGTGAATCCTCAGTTCAATGGTGCTGACTTTTTCCGACCCGAATTCGACACAAACAACGGCCCAACTGGTGGCCAGAATGATTTAGGAATGAATGAAAAAGTTTGTACTAAGAGATTGGTGTTTGAAGGTTCGAAGATAGCAGCTCTTAAAGCTATGGTTCCTGGGAAAGTAGAGAATCCAACTCGTGTTCAGCTTATTGCTGCGTTCATCTACAAAGCTGCGCTTTCTGCAAAATTTTCAGTCACAGGTAGTTTGCCAGAAACAAGTGCACTACTTCTTATGATCAACTTACGCAACAGAGTGGAGCCACCATTACCAACATTAATAGGTAACATAATCTCATTCTTCATAGCAACATCGACCACTAAGGAGCAAAGGGACATGGAGTTATGGGACATAGTGGTTGACATGAAGAGAAACTTACAAGAGTTTTGTAAAAAGTTCCCAAAAGATTACAAAGCTGAAGAATGGAGGCGTCTTTACAAATTACATGCCAAAGAATCAATGGAGAAATTGAGAAATGTGAAGGATTCTCCTAATGTGTATGCATGTACAAGTTGGTGCAAATTTCCAGTTTATGATGCTGATTTTGGATGGGGGAAGCCTATGGGTAACTGTACCTGA

Coding sequence (CDS)

ATGGGCGTCATGTTCGTGGAGGCAAAGGCCCAGTGTCCGATGTCGGAGGTTATGAACGACAACAATTTACAACGCGAAGAGATGTTGAAGTTGCTTCTTTTAGATGACATGAATAAAAAGGACCAAGGATTATTGCATCCTTTGTTGTCTATTCAGCTGACTCAATTTGAATGTGGAGGAGAAGTGATTTGTGTGTTTTTTCATCACAAGCTTGCGGATATAGCCTCCATAACCAGCTTTATGAGAGATTGGGCTTCCATTGCTCGTAGCTTCGGTGACACACATCAACCGACGGTGAATCCTCAGTTCAATGGTGCTGACTTTTTCCGACCCGAATTCGACACAAACAACGGCCCAACTGGTGGCCAGAATGATTTAGGAATGAATGAAAAAGTTTGTACTAAGAGATTGGTGTTTGAAGGTTCGAAGATAGCAGCTCTTAAAGCTATGGTTCCTGGGAAAGTAGAGAATCCAACTCGTGTTCAGCTTATTGCTGCGTTCATCTACAAAGCTGCGCTTTCTGCAAAATTTTCAGTCACAGGTAGTTTGCCAGAAACAAGTGCACTACTTCTTATGATCAACTTACGCAACAGAGTGGAGCCACCATTACCAACATTAATAGGTAACATAATCTCATTCTTCATAGCAACATCGACCACTAAGGAGCAAAGGGACATGGAGTTATGGGACATAGTGGTTGACATGAAGAGAAACTTACAAGAGTTTTGTAAAAAGTTCCCAAAAGATTACAAAGCTGAAGAATGGAGGCGTCTTTACAAATTACATGCCAAAGAATCAATGGAGAAATTGAGAAATGTGAAGGATTCTCCTAATGTGTATGCATGTACAAGTTGGTGCAAATTTCCAGTTTATGATGCTGATTTTGGATGGGGGAAGCCTATGGGTAACTGTACCTGA

Protein sequence

MGVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGGEVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRPEFDTNNGPTGGQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKFSVTGSLPETSALLLMINLRNRVEPPLPTLIGNIISFFIATSTTKEQRDMELWDIVVDMKRNLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGWGKPMGNCT
Homology
[Close]
BLAST of Cla001301 vs. Swiss-Prot
Match: VINSY_RAUSE (Vinorine synthase OS=Rauvolfia serpentina GN=ACT PE=1 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 6.2e-27
Identity = 96/308 (31.17%), Postives = 148/308 (48.05%), Query Frame = 1 

Query: 2   GVMFVEAKAQCPMSE-VMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGG 61
           GV FVEA+ Q  +S+ + N   L++ +           K +     PL +++++ FECGG
Sbjct: 93  GVPFVEARVQAQLSQAIQNVVELEKLDQYLPSAAYPGGKIEVNEDVPL-AVKISFFECGG 152

Query: 62  EVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRPEFDTNNGPT 121
             I V   HK+AD+ S+ +F+  W +  R  G+T    V P F+ A    P  D    P 
Sbjct: 153 TAIGVNLSHKIADVLSLATFLNAWTATCR--GETE--IVLPNFDLAARHFPPVDNTPSP- 212

Query: 122 GGQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVE--NPTRVQLIAAFIYKAALSAKFS 181
               +L  +E V  KR VF+  KI AL+A      E  N +RVQL+ A+I+K  +    +
Sbjct: 213 ----ELVPDENVVMKRFVFDKEKIGALRAQASSASEEKNFSRVQLVVAYIWKHVIDVTRA 272

Query: 182 VTGSLPETSALLLMINLRNRVEPPLPTL-IGNIISFFIATSTTKEQRDMELWDIVVDMKR 241
             G+      ++  +NLR+R+ PPLP   +GNI +   A    +  +D    D++  ++ 
Sbjct: 273 KYGA-KNKFVVVQAVNLRSRMNPPLPHYAMGNIATLLFAAVDAEWDKDFP--DLIGPLRT 332

Query: 242 NLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGW 301
           +L+    K   D+  E  + +  L+  E  E L          + TSWC+   YD DFGW
Sbjct: 333 SLE----KTEDDHNHELLKGMTCLYELEPQELL----------SFTSWCRLGFYDLDFGW 373

Query: 302 GKPMGNCT 306
           GKP+  CT
Sbjct: 393 GKPLSACT 373

[Close]
BLAST of Cla001301 vs. Swiss-Prot
Match: ASAT3_SOLLC (Acylsugar acyltransferase 3 OS=Solanum lycopersicum GN=ASAT3 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.2e-23
Identity = 82/304 (26.97%), Postives = 148/304 (48.68%), Query Frame = 1 

Query: 2   GVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGGE 61
           G  ++  +  CPMS+++N       +++ ++   D+      L    L +QL+ F+CGG 
Sbjct: 91  GAEYLNVRIDCPMSQILNH---PYNDVVDVVFPQDLPWSSSSLTRSPLVVQLSHFDCGGV 150

Query: 62  VICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRPEFDTNNGPTG 121
            +     H + D   ++ F+ DWAS AR+     +   +PQFN + FF    +TN   T 
Sbjct: 151 AVSACTSHTIFDGYCLSKFINDWASTARNM----EFKPSPQFNASTFFPLPSETNLSST- 210

Query: 122 GQNDLGMNEKVCTKRLVFEGSKIAALKAMV--PGKVENPTRVQLIAAFIYKAALSAKFSV 181
                  +++  ++   F  S +  LK +V     V+NPTRV++ +A ++K  ++     
Sbjct: 211 -LPATRPSQRHVSRMYNFSSSNLTRLKDIVTKESHVKNPTRVEVASALVHKCGVTMSMES 270

Query: 182 TGSLPETSALLLMINLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMKRN 241
           +G    T    LM +  N + PP+P   +GN     + T+ T++  +++L + V  ++++
Sbjct: 271 SGMFKPT----LMSHAMN-LRPPIPLNTMGNATCIILTTAMTED--EVKLPNFVAKLQKD 330

Query: 242 LQEFCKKFPKDYKAEEWRRLYKLH-AKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGW 301
            Q+   K  KD K E+   LY L   K +M  +   KD+ +VY C+      ++  DFGW
Sbjct: 331 KQQLRDKL-KDMK-EDRMPLYTLELGKNAMNIIE--KDTHDVYLCSGMTNTGLHKIDFGW 374

[Close]
BLAST of Cla001301 vs. Swiss-Prot
Match: BAHD1_ARATH (BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana GN=BAHD1 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.6e-22
Identity = 81/306 (26.47%), Postives = 144/306 (47.06%), Query Frame = 1 

Query: 2   GVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECG-G 61
           G +F EA+    +SE + + ++   ++L   L    +   +    PLLS+Q T F  G G
Sbjct: 90  GAVFTEARTNLLLSEFLRNIDINSLKILIPTLAPGESLDSR----PLLSVQATFFGSGSG 149

Query: 62  EVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRP-EFDTNNGP 121
             + +   H + D AS+++F+R WA+ AR  GD++     PQF       P +   +  P
Sbjct: 150 LAVGICVSHCICDAASVSTFVRGWAATAR--GDSNDELSTPQFAEVAIHPPADISIHGSP 209

Query: 122 TGGQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGK-VENPTRVQLIAAFIYKAALSAKFS 181
               +++   EK  T R VFE  KI  LK +   K V +PTRV+ + + I++ A +A  +
Sbjct: 210 FNALSEV--REKCVTNRFVFESDKITKLKIVAASKSVPSPTRVEAVMSLIWRCARNASHA 269

Query: 182 VTGSLPETSALLLMINLRNRVEPPL--PTLIGNIIS-FFIATSTTKEQRDMELWDIVVDM 241
               +P  + +   ++LR R+   +  P  IGN+   FF+      E   +E+ ++V + 
Sbjct: 270 NL-IVPRATMMTQSMDLRLRIPTNVLSPDAIGNLQGVFFLKRGPGSE---IEISEVVAEF 329

Query: 242 KRNLQEFCKKFPKDYKAEEWRRLYKLHAKESMEK-LRNVKDSPNVYACTSWCKFPVYDAD 301
           ++  +EF +   ++                 +   +  +K + + Y  +SWC+   Y+ D
Sbjct: 330 RKEKEEFNEMIKENVNGGHTNTTLGQKIMSGIANYMSELKPNIDTYTMSSWCRKAFYEVD 383

[Close]
BLAST of Cla001301 vs. Swiss-Prot
Match: DAT_CATRO (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus GN=DAT PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 5.1e-21
Identity = 88/318 (27.67%), Postives = 142/318 (44.65%), Query Frame = 1 

Query: 2   GVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGGE 61
           G+ FVE + +C M++++          L L     +  +D   +     +QL+ F+CGG 
Sbjct: 96  GIEFVEVRIRCRMNDILKYELRSYARDLVLPKRVTVGSEDTTAI-----VQLSHFDCGGL 155

Query: 62  VICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRPE-FDTNNGPT 121
            +     HK+AD  +I SFM+DWA+ A     +H        + + F R +       PT
Sbjct: 156 AVAFGISHKVADGGTIASFMKDWAASACYLSSSHHVPTPLLVSDSIFPRQDNIICEQFPT 215

Query: 122 GGQNDLGMNEKVCTKRLVFEGSKIAALKAM-VPGKVENPTRVQLIAAFIYKAALSAKFSV 181
                   ++    K  +F    I  LK+  V   +E PTRV+++ AF+ + A  A  S 
Sbjct: 216 --------SKNCVEKTFIFPPEAIEKLKSKAVEFGIEKPTRVEVLTAFLSRCATVAGKSA 275

Query: 182 TG------SLPETSALLLMINLRNRVEPPLPTLIGNIISFFIATSTTKEQ---RDMELWD 241
                   SLP    +L  INLR  +E P  + +GN++S + +  T KE     + E   
Sbjct: 276 AKNNNCGQSLP--FPVLQAINLRPILELPQNS-VGNLVSIYFSR-TIKENDYLNEKEYTK 335

Query: 242 IVVDMKRNLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSP----NVYACTSWC 301
           +V++  R  ++  K   ++      +  Y    +E ++ L+    S     + Y   SWC
Sbjct: 336 LVINELRKEKQKIKNLSRE------KLTYVAQMEEFVKSLKEFDISNFLDIDAYLSDSWC 390

Query: 302 KFPVYDADFGWGKPMGNC 305
           +FP YD DFGWGKP+  C
Sbjct: 396 RFPFYDVDFGWGKPIWVC 390

[Close]
BLAST of Cla001301 vs. Swiss-Prot
Match: SALAT_PAPSO (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum GN=SALAT PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.3e-19
Identity = 86/336 (25.60%), Postives = 159/336 (47.32%), Query Frame = 1 

Query: 2   GVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGGE 61
           G+ F + K +  M E M+  ++   ++L   ++     K+      L+ +Q+  F+CGG 
Sbjct: 101 GINFYKVKIRGKMCEFMSQPDVPLSQLLPSEVVSASVPKEA-----LVIVQVNMFDCGGT 160

Query: 62  VICVFFHHKLADIASITSFMRDWAS---IARSFGDTHQPT---VNPQFNGADFFRP--EF 121
            IC    HK+AD A++++F+R WAS    +RS G T   T   + P F+ A  F P    
Sbjct: 161 AICSSVSHKIADAATMSTFIRSWASTTKTSRSGGSTAAVTDQKLIPSFDSASLFPPSERL 220

Query: 122 DTNNG----PTGGQNDLGMNEKVCTKRLVFEGSKIAALKAMVP------GKVENPTRVQL 181
            + +G    P     +   ++K  +KR VF+ +KI +++  +        K    TRV++
Sbjct: 221 TSPSGMSEIPFSSTPEDTEDDKTVSKRFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEV 280

Query: 182 IAAFIYKAALSAKFSVTGSLPETSALLLMINLRNRVEPPLPTL-IGNI---ISFFIATST 241
           + + I+K+ +  K +  G LP    +   +NLR +++PPL  +  GN+   +S F+  +T
Sbjct: 281 VTSLIWKSVM--KSTPAGFLP---VVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATT 340

Query: 242 TKEQRDMELWDIVVDMKRNLQEFCKKFPKDYKAEEWRRLYKLHA-KESMEK--------- 301
           T     +   +  ++   +  +       D+ A+    + K+   K S+EK         
Sbjct: 341 TTTTNAV---NKTINSTSSESQVVLHELHDFIAQMRSEIDKVKGDKGSLEKVIQNFASGH 400

[Close]
BLAST of Cla001301 vs. TrEMBL
Match: A0A0A0KV47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001560 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 9.8e-80
Identity = 169/312 (54.17%), Postives = 222/312 (71.15%), Query Frame = 1 

Query: 1   MGVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMN--KKDQGLLHPLLSIQLTQFEC 60
           MG   +EA+ +CPMSE MN  NL+ +E LKL+  DDMN   KDQ   +PLL +QLT+FEC
Sbjct: 83  MGATILEARLRCPMSEFMNVYNLRHDEALKLVCFDDMNDGSKDQRY-NPLLCVQLTRFEC 142

Query: 61  GGEVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNP-QFNGADFFRPEFDTNN 120
           GGEV+CVF  HKLAD ++ T+FM  WAS++R+ GD   P + P +F+    F  E  T +
Sbjct: 143 GGEVLCVFLSHKLADASTFTNFMNHWASLSRT-GDHDMPLLPPPRFDAGSLF--EAATVD 202

Query: 121 GPTGGQNDLGMNEK-VCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAK 180
              G  + +   EK + +KR+VF+ SKIAALKA V  KVENP+RVQ++AAFIYKAA+SA 
Sbjct: 203 DVLGEGSKIVKREKNLVSKRMVFKASKIAALKATVCDKVENPSRVQILAAFIYKAAISAS 262

Query: 181 FSVTG----SLPETSALL-LMINLRNRVEPPLP-TLIGNIISFFIATSTTK-EQRDMELW 240
            S         P T+ LL + INLR R+ PP+P TL GNIISFF+ ++TT  EQ+++EL 
Sbjct: 263 KSTLSIHHSRTPITNYLLRIAINLRTRLIPPMPATLSGNIISFFLTSATTTAEQKEIELS 322

Query: 241 DIVVDMKRNLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFP 300
            ++ DMKRN++EFC K+P++YKAEEW  LYKLH KESME++ N +D   VYAC+SWC+FP
Sbjct: 323 SLLSDMKRNMKEFCNKYPRNYKAEEWSSLYKLHIKESMERMNNPEDQ-IVYACSSWCRFP 382

Query: 301 VYDADFGWGKPM 302
            YDADFGWGKP+
Sbjct: 383 FYDADFGWGKPV 389

[Close]
BLAST of Cla001301 vs. TrEMBL
Match: A0A0A0KT83_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001550 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 3.3e-75
Identity = 159/304 (52.30%), Postives = 205/304 (67.43%), Query Frame = 1 

Query: 1   MGVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGG 60
           +G +  EAK +CPMSE MN+ NL+REE+LKL L DD+N K++ L +PL  +QLTQFECGG
Sbjct: 53  VGAIVSEAKLRCPMSEFMNNFNLEREEILKLTLSDDINGKEKRL-NPLFCVQLTQFECGG 112

Query: 61  EVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTV--NPQFNGADFFRPEFDTNNG 120
           EVI                          S    + PT+   PQFNGA FF P+ +   G
Sbjct: 113 EVI--------------------------SREGNNPPTLICPPQFNGASFFPPKLE-GGG 172

Query: 121 PTGGQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKFS 180
               + +      V +KR VF+GSKIA+LK MV  KVENPTRVQ++ AFIYKAA+SAK S
Sbjct: 173 ELNSRTERQYENGVSSKRFVFKGSKIASLKGMVCEKVENPTRVQILTAFIYKAAVSAKES 232

Query: 181 VTGSLPETSALLLMINLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMKR 240
           VTG++  T  LL +INLRNRV+PPLP TL GN+IS+FI  S   E+++MELW++V + KR
Sbjct: 233 VTGNVAAT-ILLQIINLRNRVDPPLPATLSGNVISWFIGLSRMPEKKEMELWNLVGETKR 292

Query: 241 NLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGW 300
           N +EFCK FP +Y+AEEW  L+KLHAKESME     ++  N+Y C+SWCKFP+Y+ADFG 
Sbjct: 293 NFEEFCKTFPTNYRAEEWGLLHKLHAKESMEDRMINQEDHNLYTCSSWCKFPIYEADFGR 327

Query: 301 GKPM 302
           GKP+
Sbjct: 353 GKPV 327

[Close]
BLAST of Cla001301 vs. TrEMBL
Match: A0A0A0LNF8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G350350 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.9e-47
Identity = 118/302 (39.07%), Postives = 176/302 (58.28%), Query Frame = 1 

Query: 2   GVMFVEAKAQ-CPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGG 61
           GV FVEA+ +   +S+++ +    R E+++ L +D +  KD  +   LL +Q+T FECGG
Sbjct: 99  GVTFVEARLEGVTVSKILEN---PRSEIVEKLFVDGLQWKDSKM-GSLLKVQITLFECGG 158

Query: 62  EVICVFFHHKLADIASITSFMRDWASIARSFGDT-HQPTVNPQFNGADFFRPEFDTNNGP 121
             I V   HKL D+A++  F++DW  I R+ G    +  +NP F   D F       +G 
Sbjct: 159 LSIGVLLSHKLGDLATLVKFIQDWGVITRNNGFCDEEEIINPLFFSGDLFP------HGD 218

Query: 122 TGGQNDLGMNEKVCT-KRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKFS 181
               +   + E   T KR VFEGSKI +LK  +  KV+NP+RV++++A IYKA +SA  +
Sbjct: 219 LPAMSGAVIEEGNFTCKRFVFEGSKIESLKNRISEKVQNPSRVEVVSALIYKAIISASRT 278

Query: 182 VTGSLPETSALLLM-INLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMK 241
              S    + LLL  +NLR RV PPLP TL+G+++SFF       E+  +EL ++V  M+
Sbjct: 279 NNNSQNHPATLLLQTLNLRKRVAPPLPDTLVGSLVSFF-PVGVDGEREVVELHELVGTMR 338

Query: 242 RNLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFG 299
           + + EFC+K+ K Y+ +EW  L K    ES E L    ++  VY C+S C FP+Y+ DFG
Sbjct: 339 KEMGEFCEKYAKKYRTKEWLELIKKRLNESREILSKNGNNQLVYRCSSGCNFPIYEVDFG 389

[Close]
BLAST of Cla001301 vs. TrEMBL
Match: W9QQ26_9ROSA (BAHD acyltransferase OS=Morus notabilis GN=L484_006266 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 9.9e-40
Identity = 112/302 (37.09%), Postives = 161/302 (53.31%), Query Frame = 1 

Query: 2   GVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGGE 61
           G  F+EA+  C +SE + + +      L  LL     K  + +   +L IQLT F+CGG 
Sbjct: 100 GAYFIEARINCQLSEFIKEPD---PNFLSHLLPTSDPKGAELVGRVVLLIQLTVFKCGGT 159

Query: 62  VICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRPEFDTNNGPTG 121
            + V   HK+AD +S+  F++ WA++AR     H   V+P+F GA   RP  D    P+ 
Sbjct: 160 AVSVCASHKIADASSMCEFIKSWAAMARD----HDEVVHPEFIGASMMRPTTD----PSI 219

Query: 122 GQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVEN--PTRVQLIAAFIYKAALSAKFSV 181
                   E   TKR VF+ SK+A LKA +   V    PT V+L++A   + A++A  S 
Sbjct: 220 EGRLEAPGESFVTKRFVFDVSKLANLKAKIGSGVPQLVPTNVELVSAIFLRCAIAACQST 279

Query: 182 TGSLPETSALLLMINLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMKRN 241
           TG L + S L   +NLR R+ PP+P   IGN+I  F      +E   ME+ DIVV M++ 
Sbjct: 280 TGCL-KPSLLYQTVNLRTRMVPPVPEAAIGNLIWGFPVV--VEEASKMEVGDIVVKMRKG 339

Query: 242 LQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGWG 301
           L +FC +    +K E+          ES +   ++K   N+Y C+SWCKFP Y+ DFGWG
Sbjct: 340 LIDFCNEKANRFKGEDGYSF----VFESFKVGESLKMGMNMYFCSSWCKFPFYEVDFGWG 383

[Close]
BLAST of Cla001301 vs. TrEMBL
Match: B9RML6_RICCO (3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase, putative OS=Ricinus communis GN=RCOM_1081680 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.6e-37
Identity = 115/300 (38.33%), Postives = 169/300 (56.33%), Query Frame = 1 

Query: 2   GVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGGE 61
           GV F+EA+ +  +SE++   +   +  LK L   D+  KD  +L  LL +Q T F+CGG 
Sbjct: 95  GVEFLEARMRSNLSEILKHPD---DSTLKSLFPGDLQYKDP-ILSSLLIVQSTFFDCGGM 154

Query: 62  VICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRPEFDTNNGPTG 121
            + V   HK++DIAS+  F+  WA+IA S G+     + P+FN    + P  D     T 
Sbjct: 155 AVAVCISHKISDIASMCYFINHWAAIACSQGEK----LCPEFNLGSLY-PPIDLPVTETY 214

Query: 122 GQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKFSVTG 181
                G   K  ++RLVF+  KIA LK MV  +V NPTRV++++A +Y++A+SA    +G
Sbjct: 215 Q----GEKVKCVSRRLVFDALKIAKLKEMVAKEVSNPTRVEVVSAILYRSAISAARLRSG 274

Query: 182 SLPETSALLLMINLRNRVEPPLPTL-IGNIISFFIATSTTKEQRDMELWDIVVDMKRNLQ 241
           S   T A+    NLR RV PPLP    GNI   F  + TT E   +EL  +  ++K+   
Sbjct: 275 SSKPT-AMHNAANLRPRVLPPLPECSAGNISGTF--SVTTMEDSVIELVQLASEVKKEKT 334

Query: 242 EFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGWGKP 301
           E+C    + + AEE    + L A   + +L + KD  +VY C+S+C++P YDADFGWGKP
Sbjct: 335 EYCNSCGQKFSAEE-LCAFVLDASVGL-RLCHGKDQ-DVYLCSSFCRYPFYDADFGWGKP 375

[Close]
BLAST of Cla001301 vs. NCBI nr
Match: gi|659109007|ref|XP_008454499.1| (PREDICTED: BAHD acyltransferase At5g47980-like [Cucumis melo])

HSP 1 Score: 343.6 bits (880), Expect = 3.6e-91
Identity = 180/304 (59.21%), Postives = 226/304 (74.34%), Query Frame = 1 

Query: 1   MGVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGG 60
           +G +  EAK +CPMSE MN+ NL+REE+LKL L DD+N KDQ L +PLL +QLTQFECGG
Sbjct: 89  VGAIVSEAKLRCPMSEFMNNFNLEREEILKLTLSDDINGKDQRL-NPLLCVQLTQFECGG 148

Query: 61  EVICVFFHHKLADIASITSFMRDWASIARSFGDTHQP-TVN--PQFNGADFFRPEFDTNN 120
           EV+ V   HK+AD+AS+T+F+ DWA IARS  D + P T+N  PQFNGA FF P    + 
Sbjct: 149 EVMYVLCSHKVADLASLTNFINDWAFIARSSRDGNDPPTLNCPPQFNGASFFPPTKLESG 208

Query: 121 GPTGGQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKF 180
           G    + +      V TKR VF+ SKIA+LK MV  KVENPTRVQ++ AFIYKAA+SAK 
Sbjct: 209 GELNSRTERQYENGVSTKRFVFKSSKIASLKGMVCEKVENPTRVQILTAFIYKAAVSAKE 268

Query: 181 SVTGSLPETSALLLMINLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMK 240
           SVTGS+P T  LL  INLRNRV+PPLP TL GN+IS+FI  S T+E+R+MELW++V D K
Sbjct: 269 SVTGSVPAT-ILLQTINLRNRVDPPLPATLSGNVISWFIGLSRTQEKREMELWNLVGDTK 328

Query: 241 RNLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFG 300
           RN +E+CK FP DY++EEW  L +LHAKESME     ++  ++Y C+SWCKFP+Y+ADFG
Sbjct: 329 RNFEEYCKTFPTDYRSEEWGLLQRLHAKESMEDRMINEEDRSLYTCSSWCKFPIYEADFG 388

[Close]
BLAST of Cla001301 vs. NCBI nr
Match: gi|778697399|ref|XP_004152371.2| (PREDICTED: vinorine synthase-like [Cucumis sativus])

HSP 1 Score: 305.1 bits (780), Expect = 1.4e-79
Identity = 169/312 (54.17%), Postives = 222/312 (71.15%), Query Frame = 1 

Query: 1   MGVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMN--KKDQGLLHPLLSIQLTQFEC 60
           MG   +EA+ +CPMSE MN  NL+ +E LKL+  DDMN   KDQ   +PLL +QLT+FEC
Sbjct: 83  MGATILEARLRCPMSEFMNVYNLRHDEALKLVCFDDMNDGSKDQRY-NPLLCVQLTRFEC 142

Query: 61  GGEVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNP-QFNGADFFRPEFDTNN 120
           GGEV+CVF  HKLAD ++ T+FM  WAS++R+ GD   P + P +F+    F  E  T +
Sbjct: 143 GGEVLCVFLSHKLADASTFTNFMNHWASLSRT-GDHDMPLLPPPRFDAGSLF--EAATVD 202

Query: 121 GPTGGQNDLGMNEK-VCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAK 180
              G  + +   EK + +KR+VF+ SKIAALKA V  KVENP+RVQ++AAFIYKAA+SA 
Sbjct: 203 DVLGEGSKIVKREKNLVSKRMVFKASKIAALKATVCDKVENPSRVQILAAFIYKAAISAS 262

Query: 181 FSVTG----SLPETSALL-LMINLRNRVEPPLP-TLIGNIISFFIATSTTK-EQRDMELW 240
            S         P T+ LL + INLR R+ PP+P TL GNIISFF+ ++TT  EQ+++EL 
Sbjct: 263 KSTLSIHHSRTPITNYLLRIAINLRTRLIPPMPATLSGNIISFFLTSATTTAEQKEIELS 322

Query: 241 DIVVDMKRNLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFP 300
            ++ DMKRN++EFC K+P++YKAEEW  LYKLH KESME++ N +D   VYAC+SWC+FP
Sbjct: 323 SLLSDMKRNMKEFCNKYPRNYKAEEWSSLYKLHIKESMERMNNPEDQ-IVYACSSWCRFP 382

Query: 301 VYDADFGWGKPM 302
            YDADFGWGKP+
Sbjct: 383 FYDADFGWGKPV 389

[Close]
BLAST of Cla001301 vs. NCBI nr
Match: gi|778697396|ref|XP_011654313.1| (PREDICTED: BAHD acyltransferase At5g47980-like [Cucumis sativus])

HSP 1 Score: 296.2 bits (757), Expect = 6.5e-77
Identity = 159/304 (52.30%), Postives = 210/304 (69.08%), Query Frame = 1 

Query: 1   MGVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGG 60
           +G +  EAK +CPMSE MN+ NL+REE+LKL L DD+N K++ L +PL  +QLTQFECGG
Sbjct: 53  VGAIVSEAKLRCPMSEFMNNFNLEREEILKLTLSDDINGKEKRL-NPLFCVQLTQFECGG 112

Query: 61  EVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTV--NPQFNGADFFRPEFDTNNG 120
           EV+ V   H++AD+A I    R+           + PT+   PQFNGA FF P+ +   G
Sbjct: 113 EVMYVLCSHEVADLAFIARSSRE---------GNNPPTLICPPQFNGASFFPPKLE-GGG 172

Query: 121 PTGGQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKFS 180
               + +      V +KR VF+GSKIA+LK MV  KVENPTRVQ++ AFIYKAA+SAK S
Sbjct: 173 ELNSRTERQYENGVSSKRFVFKGSKIASLKGMVCEKVENPTRVQILTAFIYKAAVSAKES 232

Query: 181 VTGSLPETSALLLMINLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMKR 240
           VTG L      L+ IN + +V+PPLP TL GN+IS+FI  S   E+++MELW++V + KR
Sbjct: 233 VTGLLT-----LVWINFKLKVDPPLPATLSGNVISWFIGLSRMPEKKEMELWNLVGETKR 292

Query: 241 NLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGW 300
           N +EFCK FP +Y+AEEW  L+KLHAKESME     ++  N+Y C+SWCKFP+Y+ADFG 
Sbjct: 293 NFEEFCKTFPTNYRAEEWGLLHKLHAKESMEDRMINQEDHNLYTCSSWCKFPIYEADFGR 340

Query: 301 GKPM 302
           GKP+
Sbjct: 353 GKPV 340

[Close]
BLAST of Cla001301 vs. NCBI nr
Match: gi|700197630|gb|KGN52788.1| (hypothetical protein Csa_4G001550 [Cucumis sativus])

HSP 1 Score: 290.0 bits (741), Expect = 4.7e-75
Identity = 159/304 (52.30%), Postives = 205/304 (67.43%), Query Frame = 1 

Query: 1   MGVMFVEAKAQCPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGG 60
           +G +  EAK +CPMSE MN+ NL+REE+LKL L DD+N K++ L +PL  +QLTQFECGG
Sbjct: 53  VGAIVSEAKLRCPMSEFMNNFNLEREEILKLTLSDDINGKEKRL-NPLFCVQLTQFECGG 112

Query: 61  EVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTV--NPQFNGADFFRPEFDTNNG 120
           EVI                          S    + PT+   PQFNGA FF P+ +   G
Sbjct: 113 EVI--------------------------SREGNNPPTLICPPQFNGASFFPPKLE-GGG 172

Query: 121 PTGGQNDLGMNEKVCTKRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKFS 180
               + +      V +KR VF+GSKIA+LK MV  KVENPTRVQ++ AFIYKAA+SAK S
Sbjct: 173 ELNSRTERQYENGVSSKRFVFKGSKIASLKGMVCEKVENPTRVQILTAFIYKAAVSAKES 232

Query: 181 VTGSLPETSALLLMINLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMKR 240
           VTG++  T  LL +INLRNRV+PPLP TL GN+IS+FI  S   E+++MELW++V + KR
Sbjct: 233 VTGNVAAT-ILLQIINLRNRVDPPLPATLSGNVISWFIGLSRMPEKKEMELWNLVGETKR 292

Query: 241 NLQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGW 300
           N +EFCK FP +Y+AEEW  L+KLHAKESME     ++  N+Y C+SWCKFP+Y+ADFG 
Sbjct: 293 NFEEFCKTFPTNYRAEEWGLLHKLHAKESMEDRMINQEDHNLYTCSSWCKFPIYEADFGR 327

Query: 301 GKPM 302
           GKP+
Sbjct: 353 GKPV 327

[Close]
BLAST of Cla001301 vs. NCBI nr
Match: gi|659087360|ref|XP_008444410.1| (PREDICTED: vinorine synthase [Cucumis melo])

HSP 1 Score: 205.3 bits (521), Expect = 1.5e-49
Identity = 119/300 (39.67%), Postives = 178/300 (59.33%), Query Frame = 1 

Query: 2   GVMFVEAKAQ-CPMSEVMNDNNLQREEMLKLLLLDDMNKKDQGLLHPLLSIQLTQFECGG 61
           GV FVEA  +   +S+++ +    R E++++L +D +  KD  +   LL +Q+T FECGG
Sbjct: 102 GVTFVEAWLEGVTVSKILEN---PRSEIVEMLFVDGLQWKDSKM-GSLLKVQITLFECGG 161

Query: 62  EVICVFFHHKLADIASITSFMRDWASIARSFGDTHQPTVNPQFNGADFFRPEFDTNNGPT 121
             I V   HKL D+A++  F++DW  I R+ G   + TVNP F  AD F       +G  
Sbjct: 162 LSIGVLLSHKLGDLATLVKFVQDWGLITRNNGFCEEETVNPLFFSADVFP------HGDL 221

Query: 122 GGQNDLGMNEKVCT-KRLVFEGSKIAALKAMVPGKVENPTRVQLIAAFIYKAALSAKFSV 181
              +   + E   T KR VFEGSKI +LK  +  KV+NP+RV++++A IYKA +SA  + 
Sbjct: 222 PAMSGAVIEEGNFTCKRFVFEGSKIESLKNRISEKVQNPSRVEVVSALIYKAIISASRNN 281

Query: 182 TGSLPETSALLLMINLRNRVEPPLP-TLIGNIISFFIATSTTKEQRDMELWDIVVDMKRN 241
             S  +++ LL  +NLR RV PPLP TL+G+++SFF       E+  +EL ++V  M++ 
Sbjct: 282 NNSQNQSTLLLQTLNLRKRVVPPLPETLVGSLVSFF-PVGVDGEREVVELHELVGTMRKE 341

Query: 242 LQEFCKKFPKDYKAEEWRRLYKLHAKESMEKLRNVKDSPNVYACTSWCKFPVYDADFGWG 299
           + EFC ++ K Y+ +EW  L K    ES E L    ++  VY C+S C FP+Y+ DFGWG
Sbjct: 342 MGEFCDRYAKKYRTKEWLELIKKRLNESREILSKNGNNQLVYRCSSGCNFPIYEVDFGWG 390

The following BLAST results are available for this feature:
BLAST of Cla001301 vs. Swiss-Prot
Match NameE-valueIdentityDescription
VINSY_RAUSE6.2e-2731.17Vinorine synthase OS=Rauvolfia serpentina GN=ACT PE=1 SV=2[more]
ASAT3_SOLLC4.2e-2326.97Acylsugar acyltransferase 3 OS=Solanum lycopersicum GN=ASAT3 PE=2 SV=1[more]
BAHD1_ARATH4.6e-2226.47BAHD acyltransferase At5g47980 OS=Arabidopsis thaliana GN=BAHD1 PE=2 SV=1[more]
DAT_CATRO5.1e-2127.67Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus GN=DAT PE=1 SV=1[more]
SALAT_PAPSO1.3e-1925.60Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum GN=SALAT PE=1 SV=1[more]
BLAST of Cla001301 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KV47_CUCSA9.8e-8054.17Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001560 PE=4 SV=1[more]
A0A0A0KT83_CUCSA3.3e-7552.30Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001550 PE=4 SV=1[more]
A0A0A0LNF8_CUCSA4.9e-4739.07Uncharacterized protein OS=Cucumis sativus GN=Csa_2G350350 PE=4 SV=1[more]
W9QQ26_9ROSA9.9e-4037.09BAHD acyltransferase OS=Morus notabilis GN=L484_006266 PE=4 SV=1[more]
B9RML6_RICCO1.6e-3738.333'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase, putative OS=Ricinus communis ... [more]
BLAST of Cla001301 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659109007|ref|XP_008454499.1|3.6e-9159.21PREDICTED: BAHD acyltransferase At5g47980-like [Cucumis melo][more]
gi|778697399|ref|XP_004152371.2|1.4e-7954.17PREDICTED: vinorine synthase-like [Cucumis sativus][more]
gi|778697396|ref|XP_011654313.1|6.5e-7752.30PREDICTED: BAHD acyltransferase At5g47980-like [Cucumis sativus][more]
gi|700197630|gb|KGN52788.1|4.7e-7552.30hypothetical protein Csa_4G001550 [Cucumis sativus][more]
gi|659087360|ref|XP_008444410.1|1.5e-4939.67PREDICTED: vinorine synthase [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003480Transferase
IPR023213CAT-like_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0016747transferase activity, transferring acyl groups other than amino-acyl groups
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016747 transferase activity, transferring acyl groups other than amino-acyl groups
molecular_function GO:0016740 transferase activity
molecular_function GO:0016746 transferase activity, transferring acyl groups
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla001301Cla001301.1mRNA


InterPro
Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 2..301
score: 3.6
IPR023213Chloramphenicol acetyltransferase-like domainGENE3DG3DSA:3.30.559.10coord: 130..301
score: 9.2E-30coord: 2..107
score: 1.8
NoneNo IPR availablePANTHERPTHR31623FAMILY NOT NAMEDcoord: 1..301
score: 2.7