ClCG10G004110 (gene) Watermelon (Charleston Gray)

NameClCG10G004110
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionSubtilisin-like serine protease
LocationCG_Chr10 : 5265365 .. 5267190 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCCTTTAAAATTTCGGCCGCCCGGTCGAAATGGTGCTTTTCCGGCCATTCCGACCGGCGACCTCCGGTTTTTACTTACAGAAAACCCTCCGGCTGAATCTTTTTTCTATCATAACTGGCTATCTCCCAACTATCAAAATCTAGGGTGCAAGCCTTTCTTCTAAAGCATTGCCAGTTAACAAGTTCAACCCCTTAATTAATGCCGTGGATGCGAAAGCCAACAATGTTACTAACCGTGATGCGTAAGTATTTTACTCCGATGCTTTTTTTTTCCCTCAAGAGACCAGAAGAAATATGAATAGACAATTAAATAATGGACTTGCCACAATTTCCAGACGAACTTATAAAGAATTCTTACACAGGGAAATTTGTGGTGAGGGAACGCTTGATTTCACGAAATTGAATGGGAAGATTGTGGTTTGCCTTCGAGGGGTTAACTCAAGAGTGGCCAAAGGTTATGTAGCTGCTCAGGCAGGGGCTGTTGGGATGATTCTGGTTAACGATGAGCTAAGTGGGAATGAGATTTTAGCTGATGCACACATCATTCCAGCTTCCAATGTAAACTATAATGATAGCATATCCATCTCCCAATATATCAGCTCTACAACGTAACTCCCTATATTTTCTTGTTTTTTTTCCAAAGATTGCATAAATATTTTGTTTCCTCTACAAGTTTGAATTCATATTAACATGTACTTGATCAGGACACCAATGGCTTACATAAGTCCTGCGAGGACAACACTTGGAGTCAAGCCCGCCCCAACAATAATGGCTGATTTCTCAGGAAGAGGCCCTAATACAATCGAGGAGTCAATCCTCAAGGTTTATTTTTCTCAACCAAGATTTGCTATATGAAACATTTCAGTTTAAATGCTTCCGTTCAAAATCCATGCAAACTTAAGCTTAGTAATCAACAAAATCAAAAGGAAGATCATTAAACCTAACAAGTTGTGTTTTATCAGCCAGATGTAACAGCACCAGGTGTGAATATAATGGCAGCTTACCCCAGTGGAATACTGTTGTCAAATATACCAGTTGATCATCGTCGAGTTCCTTTTAAGGTAGACTCTGGCACATCCATGGCCTGCCCCCATGTTTCAGGCATTGTAGGCCTTCTCAAAACCCTAAATCCAAAATGGAGTCCAGCCGCCATTAAATCTGCAATCATGACTACAGGTTCAATCATACAATCAAATAAAAAAAAAAACATCAAGATCTTATCTCCTAGATGATTGTTCATGTAATTGAAACAATTTTCCATCCATTTCATCTCTACAGCCAAAACAAGAGACAACAACTTACATCCAATTTTAGACTCCACTGGACTCAAAGCAACTCCATTAGCATACGGCGCTGGACATGTTCATCCAAACGGTGCGATGGACCCTGGCCTCGTTTACGACATTACAATCGACGATTACCTCAATTTCTTGTGCGCTCGAGGCTACAATGCAACGCAAATAAAAAAAATTCTCAAACAATACGTTCATTTGCAATCAATTATTCAAAGTGACGGACCTCAATTACCCTTCGATCTCAGTTACCGATTTGAAAAGGGGACCCTGTGACGATCAATCGAAAGGTGAAGAATGTGGGAAGTCCAGGGAGGTAGGTTGCTCGAGTGAAGGCGCCTTTGGAAGTTTCAATTGTTGTGGAGCCAAGTATATTGGAATTTGCCGCCATGGATGAAGAGAAAAGCTTCAAGGTTGTGTTGCAAAGCACTGGAAAGGGAAATGAAGAGAGTTATGTGTTTGGAACATTGGTTTGGTCAGATGACAAACACAATGTTAGAAGCCCAATTGTTGTTAAGTTGGGGGAATAA

mRNA sequence

ATGGCCCCTTTAAAATTTCGGCCGCCCGGTCGAAATGGTGCTTTTCCGGCCATTCCGACCGGCGACCTCCGCCTTTCTTCTAAAGCATTGCCAGTTAACAAGTTCAACCCCTTAATTAATGCCGTGGATGCGAAAGCCAACAATGTTACTAACCGTGATGCACGAACTTATAAAGAATTCTTACACAGGGAAATTTGTGGTGAGGGAACGCTTGATTTCACGAAATTGAATGGGAAGATTGTGGTTTGCCTTCGAGGGGTTAACTCAAGAGTGGCCAAAGGTTATGTAGCTGCTCAGGCAGGGGCTGTTGGGATGATTCTGGTTAACGATGAGCTAAGTGGGAATGAGATTTTAGCTGATGCACACATCATTCCAGCTTCCAATGTAAACTATAATGATAGCATATCCATCTCCCAATATATCAGCTCTACAACGACACCAATGGCTTACATAAGTCCTGCGAGGACAACACTTGGAGTCAAGCCCGCCCCAACAATAATGGCTGATTTCTCAGGAAGAGGCCCTAATACAATCGAGGAGTCAATCCTCAAGCCAGATGTAACAGCACCAGGTGTGAATATAATGGCAGCTTACCCCAGTGGAATACTGTTGTCAAATATACCAGTTGATCATCGTCGAGTTCCTTTTAAGGTAGACTCTGGCACATCCATGGCCTGCCCCCATGTTTCAGGCATTGTAGGCCTTCTCAAAACCCTAAATCCAAAATGGAGTCCAGCCGCCATTAAATCTGCAATCATGACTACAGCCAAAACAAGAGACAACAACTTACATCCAATTTTAGACTCCACTGGACTCAAAGCAACTCCATTAGCATACGGCGCTGGACATGTTCATCCAAACGGTGCGATGGACCCTGGCCTCGTTTACGACATTACAATCGACGATTACCTCAATTTCTTGGGACCCTGTGACGATCAATCGAAAGGTGAAGAATGTGGGAAGTCCAGGGAGCCAAGTATATTGGAATTTGCCGCCATGGATGAAGAGAAAAGCTTCAAGGTTGTGTTGCAAAGCACTGGAAAGGGAAATGAAGAGAGTTATGTGTTTGGAACATTGGTTTGGTCAGATGACAAACACAATGTTAGAAGCCCAATTGTTGTTAAGTTGGGGGAATAA

Coding sequence (CDS)

ATGGCCCCTTTAAAATTTCGGCCGCCCGGTCGAAATGGTGCTTTTCCGGCCATTCCGACCGGCGACCTCCGCCTTTCTTCTAAAGCATTGCCAGTTAACAAGTTCAACCCCTTAATTAATGCCGTGGATGCGAAAGCCAACAATGTTACTAACCGTGATGCACGAACTTATAAAGAATTCTTACACAGGGAAATTTGTGGTGAGGGAACGCTTGATTTCACGAAATTGAATGGGAAGATTGTGGTTTGCCTTCGAGGGGTTAACTCAAGAGTGGCCAAAGGTTATGTAGCTGCTCAGGCAGGGGCTGTTGGGATGATTCTGGTTAACGATGAGCTAAGTGGGAATGAGATTTTAGCTGATGCACACATCATTCCAGCTTCCAATGTAAACTATAATGATAGCATATCCATCTCCCAATATATCAGCTCTACAACGACACCAATGGCTTACATAAGTCCTGCGAGGACAACACTTGGAGTCAAGCCCGCCCCAACAATAATGGCTGATTTCTCAGGAAGAGGCCCTAATACAATCGAGGAGTCAATCCTCAAGCCAGATGTAACAGCACCAGGTGTGAATATAATGGCAGCTTACCCCAGTGGAATACTGTTGTCAAATATACCAGTTGATCATCGTCGAGTTCCTTTTAAGGTAGACTCTGGCACATCCATGGCCTGCCCCCATGTTTCAGGCATTGTAGGCCTTCTCAAAACCCTAAATCCAAAATGGAGTCCAGCCGCCATTAAATCTGCAATCATGACTACAGCCAAAACAAGAGACAACAACTTACATCCAATTTTAGACTCCACTGGACTCAAAGCAACTCCATTAGCATACGGCGCTGGACATGTTCATCCAAACGGTGCGATGGACCCTGGCCTCGTTTACGACATTACAATCGACGATTACCTCAATTTCTTGGGACCCTGTGACGATCAATCGAAAGGTGAAGAATGTGGGAAGTCCAGGGAGCCAAGTATATTGGAATTTGCCGCCATGGATGAAGAGAAAAGCTTCAAGGTTGTGTTGCAAAGCACTGGAAAGGGAAATGAAGAGAGTTATGTGTTTGGAACATTGGTTTGGTCAGATGACAAACACAATGTTAGAAGCCCAATTGTTGTTAAGTTGGGGGAATAA

Protein sequence

MAPLKFRPPGRNGAFPAIPTGDLRLSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHPILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFLGPCDDQSKGEECGKSREPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKLGE
BLAST of ClCG10G004110 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.9e-81
Identity = 154/281 (54.80%), Postives = 193/281 (68.68%), Query Frame = 1

Query: 27  SKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLRG 86
           SK LP  K   LI+A DA   N    DA          +C +G+LD  K+ GKI+VCLRG
Sbjct: 381 SKPLPEEKMYSLISAADANVANGNVTDAL---------LCKKGSLDPKKVKGKILVCLRG 440

Query: 87  VNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTTT 146
            N+RV KG  AA AGA GM+L ND+ SGNEI++DAH++PAS ++Y D  ++  Y+SST  
Sbjct: 441 DNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKD 500

Query: 147 PMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLSN 206
           P  YI     TL  KPAP  MA FS RGPNTI   ILKPD+TAPGVNI+AA+      ++
Sbjct: 501 PKGYIKAPTATLNTKPAP-FMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTD 560

Query: 207 IPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHPI 266
           +  D+RR PF  +SGTSM+CPH+SG+VGLLKTL+P WSPAAI+SAIMTT++TR+N   P+
Sbjct: 561 LDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPM 620

Query: 267 LDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           +D +  KA P +YG+GHV PN A  PGLVYD+T  DYL+FL
Sbjct: 621 VDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFL 651


HSP 2 Score: 43.9 bits (102), Expect = 4.5e-03
Identity = 34/89 (38.20%), Postives = 40/89 (44.94%), Query Frame = 1

Query: 292 PGLVYDITIDDYLNFLGPCDDQSKG--EECGK--SREPSILEFAAMDEEKSFKVVLQSTG 351
           P L   IT+   L  +GP    +    E  G   S EP  L F    E K F++ L+   
Sbjct: 689 PNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLP 748

Query: 352 KGNEESYVFGTLVWSDDKHNVRSPIVVKL 377
                 YVFG L W+D  H VRSPIVV+L
Sbjct: 749 V-TPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of ClCG10G004110 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 3.9e-79
Identity = 150/283 (53.00%), Postives = 198/283 (69.96%), Query Frame = 1

Query: 25  LSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCL 84
           LSS ALP  KF P++ +V+AKA N +  DA         ++C  G+LD  K  GKI+VCL
Sbjct: 373 LSSTALPHAKFYPIMASVNAKAKNASALDA---------QLCKLGSLDPIKTKGKILVCL 432

Query: 85  RGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISST 144
           RG N RV KG   A  G +GM+L N  ++GN++LAD H++PA+ +   DS ++S+YIS T
Sbjct: 433 RGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQT 492

Query: 145 TTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILL 204
             P+A+I+P+RT LG+KPAP +MA FS +GP+ +   ILKPD+TAPGV+++AAY   +  
Sbjct: 493 KKPIAHITPSRTDLGLKPAP-VMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSP 552

Query: 205 SNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLH 264
           +N   D RR+ F   SGTSM+CPH+SGI GLLKT  P WSPAAI+SAIMTTA   D+   
Sbjct: 553 TNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPG 612

Query: 265 PILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           PI ++T +KATP ++GAGHV PN A++PGLVYD+ I DYLNFL
Sbjct: 613 PIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFL 645


HSP 2 Score: 61.2 bits (147), Expect = 2.8e-08
Identity = 29/53 (54.72%), Postives = 35/53 (66.04%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKL 377
           +P+ L F  + E+K+FKV+L  +     + YVFG LVWSD KH VRSPIVVKL
Sbjct: 720 KPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of ClCG10G004110 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.5e-64
Identity = 138/289 (47.75%), Postives = 182/289 (62.98%), Query Frame = 1

Query: 20  TGDLRLSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGK 79
           TG      +ALP +K  P I A    A+N TN +           +C  GTL   K+ GK
Sbjct: 354 TGVSLFKGEALP-DKLLPFIYA--GNASNATNGN-----------LCMTGTLIPEKVKGK 413

Query: 80  IVVCLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQ 139
           IV+C RG+N+RV KG V   AG VGMIL N   +G E++ADAH++PA+ V       I  
Sbjct: 414 IVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRH 473

Query: 140 YISSTTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYP 199
           Y+++   P A IS   T +GVKP+P + A FS RGPN+I  +ILKPD+ APGVNI+AA+ 
Sbjct: 474 YVTTDPNPTASISILGTVVGVKPSPVVAA-FSSRGPNSITPNILKPDLIAPGVNILAAWT 533

Query: 200 SGILLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTR 259
                + +  D RRV F + SGTSM+CPHVSG+  LLK+++P+WSPAAI+SA+MTTA   
Sbjct: 534 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 593

Query: 260 DNNLHPILD-STGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
             +  P+LD +TG  +TP  +GAGHV P  A +PGL+YD+T +DYL FL
Sbjct: 594 YKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFL 627


HSP 2 Score: 42.4 bits (98), Expect = 1.3e-02
Identity = 20/53 (37.74%), Postives = 27/53 (50.94%), Query Frame = 1

Query: 322 SREPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVV 375
           S EP++L F   +E+KS+ V           S  FG++ WSD KH V SP+ +
Sbjct: 702 SVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of ClCG10G004110 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.0e-63
Identity = 128/244 (52.46%), Postives = 161/244 (65.98%), Query Frame = 1

Query: 65  ICGEGTLDFTKLNGKIVVCLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHII 124
           +C E TLD  ++ GKIV+C RG + RVAKG V  +AG VGMIL N   +G  ++ DAH+I
Sbjct: 381 LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLI 440

Query: 125 PASNVNYNDSISISQYISSTTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILK 184
           PA  V  N+   I  Y SS   P+A I    T +G+KPAP I A FSGRGPN +   ILK
Sbjct: 441 PACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVI-ASFSGRGPNGLSPEILK 500

Query: 185 PDVTAPGVNIMAAYPSGILLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWS 244
           PD+ APGVNI+AA+   +  + +P D R+  F + SGTSMACPHVSG   LLK+ +P WS
Sbjct: 501 PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 560

Query: 245 PAAIKSAIMTTAKTRDNNLHPILD-STGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDY 304
           PA I+SA+MTT    DN+   ++D STG  ATP  YG+GH++   AM+PGLVYDIT DDY
Sbjct: 561 PAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDY 620

Query: 305 LNFL 308
           + FL
Sbjct: 621 ITFL 623

BLAST of ClCG10G004110 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.0e-63
Identity = 141/328 (42.99%), Postives = 186/328 (56.71%), Query Frame = 1

Query: 65  ICGEGTLDFTKLNGKIVVCLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHII 124
           +C EG+LD   + GKIV+C RG+NSR  KG +  + G +GMI+ N    G  ++AD H++
Sbjct: 386 LCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVL 445

Query: 125 PASNVNYNDSISISQYIS------STTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTI 184
           PA++V  +    I +YIS      S+  P A I    T LG++PAP ++A FS RGPN  
Sbjct: 446 PATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAP-VVASFSARGPNPE 505

Query: 185 EESILKPDVTAPGVNIMAAYPSGILLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKT 244
              ILKPDV APG+NI+AA+P  I  S +  D+RR  F + SGTSMACPHVSG+  LLK 
Sbjct: 506 TPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKA 565

Query: 245 LNPKWSPAAIKSAIMTTAKTRDNNLHPILD-STGLKATPLAYGAGHVHPNGAMDPGLVYD 304
            +P WSPAAI+SA++TTA T DN+  P++D STG  ++ + YG+GHVHP  AMDPGLVYD
Sbjct: 566 AHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYD 625

Query: 305 ITIDDYLNFL-----------------GPCDDQSKGEECGKSREPSILEFAAMDEEKSFK 364
           IT  DY+NFL                   CD   +    G    P            SF 
Sbjct: 626 ITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYP------------SFS 685

Query: 365 VVLQSTGKGNEESYVFGTLVWSDDKHNV 369
           VV Q  G+    ++   T+    D  +V
Sbjct: 686 VVFQQYGESKMSTHFIRTVTNVGDSDSV 700


HSP 2 Score: 37.4 bits (85), Expect = 4.3e-01
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQST------GKGNEESYVFGTLVWSDDKHNVRSPIVVKL 377
           EP  L F  + ++ SF V +++T      G  N E+   G +VWSD K NV SP+VV L
Sbjct: 716 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET---GHIVWSDGKRNVTSPLVVTL 771

BLAST of ClCG10G004110 vs. TrEMBL
Match: A0A0A0L617_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045160 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.3e-110
Identity = 216/316 (68.35%), Postives = 249/316 (78.80%), Query Frame = 1

Query: 26  SSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLR 85
           SSK LPVNKF PLINAVDAKANNV+  DA         E+C EG+LD  KL GKIVVCLR
Sbjct: 272 SSKVLPVNKFYPLINAVDAKANNVSVSDA---------EVCDEGSLDPEKLAGKIVVCLR 331

Query: 86  GVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTT 145
           G   RV+KGYVAA+AGAVGM++VNDE SGN IL D+H++PAS+V Y+DSISI QYI+ST 
Sbjct: 332 GGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTK 391

Query: 146 TPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLS 205
           TPMAYIS   T L + P+P ++ADFS RGPNTIEESILKPD+ APGVNI+AAYP GI L+
Sbjct: 392 TPMAYISSVMTELEITPSP-VVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLT 451

Query: 206 NIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHP 265
             P+D R+ PFKVDSGTSMACPH++GIVGLLKTLNPKWSPAAIKSAIMTTAKT DNN +P
Sbjct: 452 EAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNP 511

Query: 266 ILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFLGPCDDQSKGEECGKSREP 325
           I+D  GL+A PLAYGAGHV+PN AMDPGLVYDITIDDYLNFL  C   ++G    + +  
Sbjct: 512 IVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFL--C---ARGYNTTQIKRI 569

Query: 326 SILEFAAMDEEKSFKV 342
           S   F     +KSFKV
Sbjct: 572 SKKNFVC---DKSFKV 569

BLAST of ClCG10G004110 vs. TrEMBL
Match: A0A0A0L617_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045160 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 8.3e-12
Identity = 37/55 (67.27%), Postives = 44/55 (80.00%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKLGE 379
           EP IL+F AMDEEKSFKV+L  +GKG +E YVFG LVW+D   +VR+PIVV LGE
Sbjct: 617 EPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDVNRHVRTPIVVNLGE 671


HSP 2 Score: 347.8 bits (891), Expect = 1.6e-92
Identity = 171/283 (60.42%), Postives = 216/283 (76.33%), Query Frame = 1

Query: 25  LSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCL 84
           +SS AL   KF PLINAVDAKA N +          +  ++C +G+LD TK  GKI+VCL
Sbjct: 346 ISSSALAGGKFYPLINAVDAKAANASE---------ILAQLCHKGSLDPTKAKGKIIVCL 405

Query: 85  RGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISST 144
           RG N+RV KG+V  QAG VGMILVN +  G+   ADAHI+PA++++Y D ++++QYI+ST
Sbjct: 406 RGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTADAHILPATHLSYTDGLAVAQYINST 465

Query: 145 TTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILL 204
            TP+A+I+P +T LG+KP+P +MADFS RGPN I E++LKPD+T PG++I+A+  + +  
Sbjct: 466 KTPVAHITPVQTQLGIKPSP-VMADFSSRGPNPITEAMLKPDITGPGMSILASVTTDVTA 525

Query: 205 SNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLH 264
           +  P D RRVPF V+SGTSM+CPH+SG+VGLLKTL P WSPAAIKSAIMTTAKTRDN + 
Sbjct: 526 TTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTMR 585

Query: 265 PILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
            I D+   KATP  YGAGHVHPN AMDPGLVYD TIDDYLNFL
Sbjct: 586 TISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTIDDYLNFL 618

BLAST of ClCG10G004110 vs. TrEMBL
Match: A0A0A0L601_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045060 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.0e-10
Identity = 34/54 (62.96%), Postives = 42/54 (77.78%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKLG 378
           EPS L+F ++ EEK+FKVV +  G   ++ YVFGTL+WSD KHNVRSPIVV LG
Sbjct: 693 EPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWSDGKHNVRSPIVVNLG 746


HSP 2 Score: 347.4 bits (890), Expect = 2.1e-92
Identity = 174/283 (61.48%), Postives = 216/283 (76.33%), Query Frame = 1

Query: 25  LSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCL 84
           LS+KAL + KF PL++A+DAKA N +  DA          +C   TLD  K+ GKI+VCL
Sbjct: 354 LSAKALQIGKFYPLVSALDAKAANASAADAL---------LCKPATLDPKKVTGKILVCL 413

Query: 85  RGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISST 144
           RG N+RV KG  A +AGAVGMIL N+EL+GNEI+AD H++PAS++NYND I++  YI+ST
Sbjct: 414 RGQNARVDKGQQALEAGAVGMILANNELTGNEIIADPHVLPASHINYNDGINVFTYINST 473

Query: 145 TTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILL 204
            +P  YI+PA T LG KPAP  MA FS +GPNT+   ILKPD+TAPGV+I+AAY      
Sbjct: 474 KSPKGYITPATTKLGTKPAP-FMAAFSSKGPNTVTPEILKPDITAPGVSIIAAYTEAQGP 533

Query: 205 SNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLH 264
           +N   D RR+ F   SGTSM+CPHVSGIVGLLKTL+P WSPAAIKSAIMTTA+TRDN + 
Sbjct: 534 TNEEFDKRRISFNSISGTSMSCPHVSGIVGLLKTLHPDWSPAAIKSAIMTTARTRDNQME 593

Query: 265 PILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           P+L+S+  KATP +YGAGHV+PNGAMDPGLVYD++ +DYLNFL
Sbjct: 594 PLLNSSNFKATPFSYGAGHVNPNGAMDPGLVYDLSFNDYLNFL 626

BLAST of ClCG10G004110 vs. TrEMBL
Match: W9S7B9_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_006884 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.7e-04
Identity = 27/54 (50.00%), Postives = 34/54 (62.96%), Query Frame = 1

Query: 322 SREPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVK 376
           S EP  L+F  + EEKSF + L++      + Y FG L+WSD  H VRSPIVVK
Sbjct: 697 SVEPKSLKFKRVGEEKSFTLTLKAKNPKAAKDYAFGKLIWSDGTHYVRSPIVVK 750


HSP 2 Score: 347.1 bits (889), Expect = 2.8e-92
Identity = 177/283 (62.54%), Postives = 216/283 (76.33%), Query Frame = 1

Query: 25  LSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCL 84
           +S K LPV KF PLI+++DAKA NVT+ DA          +C EG+LD  K+NGKI++CL
Sbjct: 357 VSDKKLPVQKFYPLISSLDAKAKNVTDNDAL---------LCEEGSLDPKKVNGKIIICL 416

Query: 85  RGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISST 144
           RG N+RVAKGYVAA+AGAVGMIL N E +G+EILADAH++PAS++ Y+D   + QYI+ST
Sbjct: 417 RGDNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINST 476

Query: 145 TTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILL 204
             PMAY++  RT  G+KPAP +MA FS RGPNT++ SILKPD+TAPG NI+AAY      
Sbjct: 477 KIPMAYMTHVRTEEGIKPAP-VMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASP 536

Query: 205 SNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLH 264
           +    D RRVPF V+SGTSM+CPHVSGIVGLLKTL PKWSPAAI+SAIMTTA T+ N+L 
Sbjct: 537 TGTDFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLT 596

Query: 265 PILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           PIL +   KA   AYGAGHV PN A DPGLVYD++  DYLN+L
Sbjct: 597 PILSTNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYL 629

BLAST of ClCG10G004110 vs. TrEMBL
Match: A0A0A0L2R9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 7.3e-08
Identity = 34/57 (59.65%), Postives = 42/57 (73.68%), Query Frame = 1

Query: 322 SREPSILEFAAMDEEKSFKVVLQS-TGKGNEESYVFGTLVWSDDKHNVRSPIVVKLG 378
           S EP+ L+F  +DEEKSF+VVL+S    G+   Y+FG L WSD KH VRSPIVV+LG
Sbjct: 703 SVEPTSLKFTGIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIVVRLG 759


HSP 2 Score: 345.9 bits (886), Expect = 6.2e-92
Identity = 173/285 (60.70%), Postives = 217/285 (76.14%), Query Frame = 1

Query: 23  LRLSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVV 82
           + LS+K LP NKF P+I+++DAKA N + +DA          +C  GTL+  K+ GKI+V
Sbjct: 369 MSLSTKGLPSNKFYPVISSLDAKAANASAQDAI---------LCKPGTLNPKKVKGKILV 428

Query: 83  CLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYIS 142
           CLRG N RV KG  AA AGAVG IL ND  SGNE++AD H++PAS+VN++D  ++  YI+
Sbjct: 429 CLRGENPRVDKGEQAALAGAVGFILANDMQSGNELIADPHVLPASHVNFSDGAAVFNYIN 488

Query: 143 STTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGI 202
           ST  PMAY++  RT LG+KPAP  MA FS +GPNTI   ILKPD+TAPGVNI+AAY   I
Sbjct: 489 STKNPMAYLTRVRTQLGIKPAP-FMASFSSKGPNTITPEILKPDITAPGVNIIAAYSESI 548

Query: 203 LLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNN 262
             ++   D RR+PF   SGTSM+CPH+SGIVGLLKTL+P WSPAAIKSAIMT+A+TRD+N
Sbjct: 549 GPTDQTFDKRRIPFNAQSGTSMSCPHISGIVGLLKTLHPDWSPAAIKSAIMTSARTRDDN 608

Query: 263 LHPILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           + P+L+S+ LKATP +YGAGHV PN AMDPGLVYD T++DYLNFL
Sbjct: 609 MEPMLNSSNLKATPFSYGAGHVRPNRAMDPGLVYDSTVNDYLNFL 643

BLAST of ClCG10G004110 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 304.3 bits (778), Expect = 1.1e-82
Identity = 154/281 (54.80%), Postives = 193/281 (68.68%), Query Frame = 1

Query: 27  SKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLRG 86
           SK LP  K   LI+A DA   N    DA          +C +G+LD  K+ GKI+VCLRG
Sbjct: 381 SKPLPEEKMYSLISAADANVANGNVTDAL---------LCKKGSLDPKKVKGKILVCLRG 440

Query: 87  VNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTTT 146
            N+RV KG  AA AGA GM+L ND+ SGNEI++DAH++PAS ++Y D  ++  Y+SST  
Sbjct: 441 DNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKD 500

Query: 147 PMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLSN 206
           P  YI     TL  KPAP  MA FS RGPNTI   ILKPD+TAPGVNI+AA+      ++
Sbjct: 501 PKGYIKAPTATLNTKPAP-FMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTD 560

Query: 207 IPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHPI 266
           +  D+RR PF  +SGTSM+CPH+SG+VGLLKTL+P WSPAAI+SAIMTT++TR+N   P+
Sbjct: 561 LDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPM 620

Query: 267 LDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           +D +  KA P +YG+GHV PN A  PGLVYD+T  DYL+FL
Sbjct: 621 VDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFL 651


HSP 2 Score: 43.9 bits (102), Expect = 2.6e-04
Identity = 34/89 (38.20%), Postives = 40/89 (44.94%), Query Frame = 1

Query: 292 PGLVYDITIDDYLNFLGPCDDQSKG--EECGK--SREPSILEFAAMDEEKSFKVVLQSTG 351
           P L   IT+   L  +GP    +    E  G   S EP  L F    E K F++ L+   
Sbjct: 689 PNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLP 748

Query: 352 KGNEESYVFGTLVWSDDKHNVRSPIVVKL 377
                 YVFG L W+D  H VRSPIVV+L
Sbjct: 749 V-TPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of ClCG10G004110 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 296.6 bits (758), Expect = 2.2e-80
Identity = 150/283 (53.00%), Postives = 198/283 (69.96%), Query Frame = 1

Query: 25  LSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCL 84
           LSS ALP  KF P++ +V+AKA N +  DA         ++C  G+LD  K  GKI+VCL
Sbjct: 373 LSSTALPHAKFYPIMASVNAKAKNASALDA---------QLCKLGSLDPIKTKGKILVCL 432

Query: 85  RGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISST 144
           RG N RV KG   A  G +GM+L N  ++GN++LAD H++PA+ +   DS ++S+YIS T
Sbjct: 433 RGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQT 492

Query: 145 TTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILL 204
             P+A+I+P+RT LG+KPAP +MA FS +GP+ +   ILKPD+TAPGV+++AAY   +  
Sbjct: 493 KKPIAHITPSRTDLGLKPAP-VMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSP 552

Query: 205 SNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLH 264
           +N   D RR+ F   SGTSM+CPH+SGI GLLKT  P WSPAAI+SAIMTTA   D+   
Sbjct: 553 TNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPG 612

Query: 265 PILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           PI ++T +KATP ++GAGHV PN A++PGLVYD+ I DYLNFL
Sbjct: 613 PIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFL 645


HSP 2 Score: 61.2 bits (147), Expect = 1.5e-09
Identity = 29/53 (54.72%), Postives = 35/53 (66.04%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKL 377
           +P+ L F  + E+K+FKV+L  +     + YVFG LVWSD KH VRSPIVVKL
Sbjct: 720 KPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of ClCG10G004110 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-65
Identity = 138/289 (47.75%), Postives = 182/289 (62.98%), Query Frame = 1

Query: 20  TGDLRLSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGK 79
           TG      +ALP +K  P I A    A+N TN +           +C  GTL   K+ GK
Sbjct: 354 TGVSLFKGEALP-DKLLPFIYA--GNASNATNGN-----------LCMTGTLIPEKVKGK 413

Query: 80  IVVCLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQ 139
           IV+C RG+N+RV KG V   AG VGMIL N   +G E++ADAH++PA+ V       I  
Sbjct: 414 IVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRH 473

Query: 140 YISSTTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYP 199
           Y+++   P A IS   T +GVKP+P + A FS RGPN+I  +ILKPD+ APGVNI+AA+ 
Sbjct: 474 YVTTDPNPTASISILGTVVGVKPSPVVAA-FSSRGPNSITPNILKPDLIAPGVNILAAWT 533

Query: 200 SGILLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTR 259
                + +  D RRV F + SGTSM+CPHVSG+  LLK+++P+WSPAAI+SA+MTTA   
Sbjct: 534 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 593

Query: 260 DNNLHPILD-STGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
             +  P+LD +TG  +TP  +GAGHV P  A +PGL+YD+T +DYL FL
Sbjct: 594 YKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFL 627


HSP 2 Score: 42.4 bits (98), Expect = 7.4e-04
Identity = 20/53 (37.74%), Postives = 27/53 (50.94%), Query Frame = 1

Query: 322 SREPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVV 375
           S EP++L F   +E+KS+ V           S  FG++ WSD KH V SP+ +
Sbjct: 702 SVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of ClCG10G004110 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 245.4 bits (625), Expect = 5.8e-65
Identity = 128/244 (52.46%), Postives = 161/244 (65.98%), Query Frame = 1

Query: 65  ICGEGTLDFTKLNGKIVVCLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHII 124
           +C E TLD  ++ GKIV+C RG + RVAKG V  +AG VGMIL N   +G  ++ DAH+I
Sbjct: 381 LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLI 440

Query: 125 PASNVNYNDSISISQYISSTTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILK 184
           PA  V  N+   I  Y SS   P+A I    T +G+KPAP I A FSGRGPN +   ILK
Sbjct: 441 PACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVI-ASFSGRGPNGLSPEILK 500

Query: 185 PDVTAPGVNIMAAYPSGILLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWS 244
           PD+ APGVNI+AA+   +  + +P D R+  F + SGTSMACPHVSG   LLK+ +P WS
Sbjct: 501 PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 560

Query: 245 PAAIKSAIMTTAKTRDNNLHPILD-STGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDY 304
           PA I+SA+MTT    DN+   ++D STG  ATP  YG+GH++   AM+PGLVYDIT DDY
Sbjct: 561 PAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDY 620

Query: 305 LNFL 308
           + FL
Sbjct: 621 ITFL 623

BLAST of ClCG10G004110 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 245.4 bits (625), Expect = 5.8e-65
Identity = 141/328 (42.99%), Postives = 186/328 (56.71%), Query Frame = 1

Query: 65  ICGEGTLDFTKLNGKIVVCLRGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHII 124
           +C EG+LD   + GKIV+C RG+NSR  KG +  + G +GMI+ N    G  ++AD H++
Sbjct: 386 LCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVL 445

Query: 125 PASNVNYNDSISISQYIS------STTTPMAYISPARTTLGVKPAPTIMADFSGRGPNTI 184
           PA++V  +    I +YIS      S+  P A I    T LG++PAP ++A FS RGPN  
Sbjct: 446 PATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAP-VVASFSARGPNPE 505

Query: 185 EESILKPDVTAPGVNIMAAYPSGILLSNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKT 244
              ILKPDV APG+NI+AA+P  I  S +  D+RR  F + SGTSMACPHVSG+  LLK 
Sbjct: 506 TPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKA 565

Query: 245 LNPKWSPAAIKSAIMTTAKTRDNNLHPILD-STGLKATPLAYGAGHVHPNGAMDPGLVYD 304
            +P WSPAAI+SA++TTA T DN+  P++D STG  ++ + YG+GHVHP  AMDPGLVYD
Sbjct: 566 AHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYD 625

Query: 305 ITIDDYLNFL-----------------GPCDDQSKGEECGKSREPSILEFAAMDEEKSFK 364
           IT  DY+NFL                   CD   +    G    P            SF 
Sbjct: 626 ITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYP------------SFS 685

Query: 365 VVLQSTGKGNEESYVFGTLVWSDDKHNV 369
           VV Q  G+    ++   T+    D  +V
Sbjct: 686 VVFQQYGESKMSTHFIRTVTNVGDSDSV 700


HSP 2 Score: 37.4 bits (85), Expect = 2.4e-02
Identity = 24/59 (40.68%), Postives = 31/59 (52.54%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQST------GKGNEESYVFGTLVWSDDKHNVRSPIVVKL 377
           EP  L F  + ++ SF V +++T      G  N E+   G +VWSD K NV SP+VV L
Sbjct: 716 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVET---GHIVWSDGKRNVTSPLVVTL 771

BLAST of ClCG10G004110 vs. NCBI nr
Match: gi|778688356|ref|XP_011652728.1| (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus])

HSP 1 Score: 407.1 bits (1045), Expect = 3.3e-110
Identity = 216/316 (68.35%), Postives = 249/316 (78.80%), Query Frame = 1

Query: 26  SSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLR 85
           SSK LPVNKF PLINAVDAKANNV+  DA         E+C EG+LD  KL GKIVVCLR
Sbjct: 233 SSKVLPVNKFYPLINAVDAKANNVSVSDA---------EVCDEGSLDPEKLAGKIVVCLR 292

Query: 86  GVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTT 145
           G   RV+KGYVAA+AGAVGM++VNDE SGN IL D+H++PAS+V Y+DSISI QYI+ST 
Sbjct: 293 GGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTK 352

Query: 146 TPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLS 205
           TPMAYIS   T L + P+P ++ADFS RGPNTIEESILKPD+ APGVNI+AAYP GI L+
Sbjct: 353 TPMAYISSVMTELEITPSP-VVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLT 412

Query: 206 NIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHP 265
             P+D R+ PFKVDSGTSMACPH++GIVGLLKTLNPKWSPAAIKSAIMTTAKT DNN +P
Sbjct: 413 EAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNP 472

Query: 266 ILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFLGPCDDQSKGEECGKSREP 325
           I+D  GL+A PLAYGAGHV+PN AMDPGLVYDITIDDYLNFL  C   ++G    + +  
Sbjct: 473 IVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFL--C---ARGYNTTQIKRI 530

Query: 326 SILEFAAMDEEKSFKV 342
           S   F     +KSFKV
Sbjct: 533 SKKNFVC---DKSFKV 530

BLAST of ClCG10G004110 vs. NCBI nr
Match: gi|778688356|ref|XP_011652728.1| (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus])

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-11
Identity = 37/55 (67.27%), Postives = 44/55 (80.00%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKLGE 379
           EP IL+F AMDEEKSFKV+L  +GKG +E YVFG LVW+D   +VR+PIVV LGE
Sbjct: 578 EPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDVNRHVRTPIVVNLGE 632


HSP 2 Score: 407.1 bits (1045), Expect = 3.3e-110
Identity = 216/316 (68.35%), Postives = 249/316 (78.80%), Query Frame = 1

Query: 26  SSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLR 85
           SSK LPVNKF PLINAVDAKANNV+  DA         E+C EG+LD  KL GKIVVCLR
Sbjct: 272 SSKVLPVNKFYPLINAVDAKANNVSVSDA---------EVCDEGSLDPEKLAGKIVVCLR 331

Query: 86  GVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTT 145
           G   RV+KGYVAA+AGAVGM++VNDE SGN IL D+H++PAS+V Y+DSISI QYI+ST 
Sbjct: 332 GGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTK 391

Query: 146 TPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLS 205
           TPMAYIS   T L + P+P ++ADFS RGPNTIEESILKPD+ APGVNI+AAYP GI L+
Sbjct: 392 TPMAYISSVMTELEITPSP-VVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLT 451

Query: 206 NIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHP 265
             P+D R+ PFKVDSGTSMACPH++GIVGLLKTLNPKWSPAAIKSAIMTTAKT DNN +P
Sbjct: 452 EAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNP 511

Query: 266 ILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFLGPCDDQSKGEECGKSREP 325
           I+D  GL+A PLAYGAGHV+PN AMDPGLVYDITIDDYLNFL  C   ++G    + +  
Sbjct: 512 IVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFL--C---ARGYNTTQIKRI 569

Query: 326 SILEFAAMDEEKSFKV 342
           S   F     +KSFKV
Sbjct: 572 SKKNFVC---DKSFKV 569

BLAST of ClCG10G004110 vs. NCBI nr
Match: gi|700200869|gb|KGN56002.1| (hypothetical protein Csa_3G045160 [Cucumis sativus])

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-11
Identity = 37/55 (67.27%), Postives = 44/55 (80.00%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKLGE 379
           EP IL+F AMDEEKSFKV+L  +GKG +E YVFG LVW+D   +VR+PIVV LGE
Sbjct: 617 EPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDVNRHVRTPIVVNLGE 671


HSP 2 Score: 399.8 bits (1026), Expect = 5.2e-108
Identity = 205/282 (72.70%), Postives = 232/282 (82.27%), Query Frame = 1

Query: 26  SSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLR 85
           SSK LPVNKF PLINAVDAKA NV+  DA         E+C EG+LD  KL GKIVVCLR
Sbjct: 282 SSKVLPVNKFYPLINAVDAKAKNVSVSDA---------EVCDEGSLDPKKLAGKIVVCLR 341

Query: 86  GVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTT 145
           G  SRV+KGYVAA+AGAVGMI+ NDE SGN I+ D+H++PAS+V Y+DSISI QYI+ST 
Sbjct: 342 GGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTK 401

Query: 146 TPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLS 205
            P AYIS   T L + P+  ++ADFS RGPNTIEESILKPD+TAPGVNI+AAYP GI L+
Sbjct: 402 RPKAYISSVMTELEITPS-AVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLT 461

Query: 206 NIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHP 265
             P+D R+ PFKVDSGTSMACPHV+GIVGLLKTLNPKWSPAAIKSAIMTTAKT DNN +P
Sbjct: 462 EAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNP 521

Query: 266 ILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           I+D  GL+A PLAYGAGHV+PN AMDPGLVYDITIDDYLNFL
Sbjct: 522 IVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFL 553

BLAST of ClCG10G004110 vs. NCBI nr
Match: gi|659096268|ref|XP_008449005.1| (PREDICTED: subtilisin-like protease isoform X2 [Cucumis melo])

HSP 1 Score: 82.4 bits (202), Expect = 1.8e-12
Identity = 39/55 (70.91%), Postives = 45/55 (81.82%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKLGE 379
           EP ILEF AMDEEKSFKV+L+ +GKG +E YVFG LVW+D  H+VRS IVV LGE
Sbjct: 627 EPRILEFTAMDEEKSFKVLLKRSGKGKQEGYVFGELVWTDFNHHVRSSIVVNLGE 681


HSP 2 Score: 399.8 bits (1026), Expect = 5.2e-108
Identity = 205/282 (72.70%), Postives = 232/282 (82.27%), Query Frame = 1

Query: 26  SSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCLR 85
           SSK LPVNKF PLINAVDAKA NV+  DA         E+C EG+LD  KL GKIVVCLR
Sbjct: 311 SSKVLPVNKFYPLINAVDAKAKNVSVSDA---------EVCDEGSLDPKKLAGKIVVCLR 370

Query: 86  GVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISSTT 145
           G  SRV+KGYVAA+AGAVGMI+ NDE SGN I+ D+H++PAS+V Y+DSISI QYI+ST 
Sbjct: 371 GGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTK 430

Query: 146 TPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILLS 205
            P AYIS   T L + P+  ++ADFS RGPNTIEESILKPD+TAPGVNI+AAYP GI L+
Sbjct: 431 RPKAYISSVMTELEITPS-AVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLT 490

Query: 206 NIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLHP 265
             P+D R+ PFKVDSGTSMACPHV+GIVGLLKTLNPKWSPAAIKSAIMTTAKT DNN +P
Sbjct: 491 EAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNP 550

Query: 266 ILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           I+D  GL+A PLAYGAGHV+PN AMDPGLVYDITIDDYLNFL
Sbjct: 551 IVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFL 582

BLAST of ClCG10G004110 vs. NCBI nr
Match: gi|659096264|ref|XP_008449002.1| (PREDICTED: subtilisin-like protease isoform X1 [Cucumis melo])

HSP 1 Score: 82.4 bits (202), Expect = 1.8e-12
Identity = 39/55 (70.91%), Postives = 45/55 (81.82%), Query Frame = 1

Query: 324 EPSILEFAAMDEEKSFKVVLQSTGKGNEESYVFGTLVWSDDKHNVRSPIVVKLGE 379
           EP ILEF AMDEEKSFKV+L+ +GKG +E YVFG LVW+D  H+VRS IVV LGE
Sbjct: 656 EPRILEFTAMDEEKSFKVLLKRSGKGKQEGYVFGELVWTDFNHHVRSSIVVNLGE 710


HSP 2 Score: 362.1 bits (928), Expect = 1.2e-96
Identity = 183/283 (64.66%), Postives = 220/283 (77.74%), Query Frame = 1

Query: 25  LSSKALPVNKFNPLINAVDAKANNVTNRDARTYKEFLHREICGEGTLDFTKLNGKIVVCL 84
           LS K LP  KF PLINA DAKANNV++  A         ++C  G+LD  K+ GKI++CL
Sbjct: 372 LSDKILPEQKFYPLINAADAKANNVSSDVA---------QLCQAGSLDPKKVKGKIILCL 431

Query: 85  RGVNSRVAKGYVAAQAGAVGMILVNDELSGNEILADAHIIPASNVNYNDSISISQYISST 144
           RG N+RV KGY AAQAGAVGMIL N E +G+E++ADAH++P S+V+Y D  SI QYI+ T
Sbjct: 432 RGENARVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFT 491

Query: 145 TTPMAYISPARTTLGVKPAPTIMADFSGRGPNTIEESILKPDVTAPGVNIMAAYPSGILL 204
            TPMAY++  RT LG+KPAP +MA FS RGPNTIEESILKPD+TAPGVNI+AAY      
Sbjct: 492 KTPMAYMTHVRTELGIKPAP-VMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASP 551

Query: 205 SNIPVDHRRVPFKVDSGTSMACPHVSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNNLH 264
           S    D+RR+PF + SGTSM+CPH+SGIVGLLKTL P WSPAAIKSAIMTTA+TR N+LH
Sbjct: 552 SGSLFDNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLH 611

Query: 265 PILDSTGLKATPLAYGAGHVHPNGAMDPGLVYDITIDDYLNFL 308
           PIL++  LKA P AYGAGHV PN AM+PGLVYD+T +DY+NFL
Sbjct: 612 PILNTVNLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFL 644

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT54_ARATH1.9e-8154.80Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1[more]
AIR3_ARATH3.9e-7953.00Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1[more]
SBT17_ARATH3.5e-6447.75Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT16_ARATH1.0e-6352.46Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1[more]
SBT15_ARATH1.0e-6342.99Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L617_CUCSA2.3e-11068.35Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045160 PE=4 SV=1[more]
A0A0A0L617_CUCSA8.3e-1267.27Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045160 PE=4 SV=1[more]
A0A0A0L601_CUCSA2.0e-1062.96Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045060 PE=4 SV=1[more]
W9S7B9_9ROSA1.7e-0450.00Subtilisin-like protease OS=Morus notabilis GN=L484_006884 PE=4 SV=1[more]
A0A0A0L2R9_CUCSA7.3e-0859.65Uncharacterized protein OS=Cucumis sativus GN=Csa_3G045120 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59810.11.1e-8254.80 Subtilase family protein[more]
AT2G04160.12.2e-8053.00 Subtilisin-like serine endopeptidase family protein[more]
AT5G67360.12.0e-6547.75 Subtilase family protein[more]
AT4G34980.15.8e-6552.46 subtilisin-like serine protease 2[more]
AT3G14240.15.8e-6542.99 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|778688356|ref|XP_011652728.1|3.3e-11068.35PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus][more]
gi|778688356|ref|XP_011652728.1|1.2e-1167.27PREDICTED: subtilisin-like protease SBT5.3 [Cucumis sativus][more]
gi|700200869|gb|KGN56002.1|1.2e-1167.27hypothetical protein Csa_3G045160 [Cucumis sativus][more]
gi|659096268|ref|XP_008449005.1|1.8e-1270.91PREDICTED: subtilisin-like protease isoform X2 [Cucumis melo][more]
gi|659096264|ref|XP_008449002.1|1.8e-1270.91PREDICTED: subtilisin-like protease isoform X1 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
cellular_component GO:0005618 cell wall
cellular_component GO:0031410 cytoplasmic vesicle
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG10G004110.1ClCG10G004110.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 165..304
score: 1.9
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 132..263
score: 3.8
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 128..294
score: 1.07
IPR003137PA domainPFAMPF02225PAcoord: 72..133
score: 5.6
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 63..375
score: 7.6E-143coord: 37..47
score: 7.6E
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 221..231
scor
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 75..122
score: 1.
NoneNo IPR availablePANTHERPTHR10795:SF3SUBFAMILY NOT NAMEDcoord: 63..375
score: 7.6E-143coord: 37..47
score: 7.6E
NoneNo IPR availableunknownSSF52025PA domaincoord: 72..115
score: 1.4

The following gene(s) are paralogous to this gene:

None