ClCG08G015850 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG08G015850
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	Calcium-binding EF-hand family protein LENGTH=170
Location	CG_Chr08 : 28283195 .. 28283458 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCGTGCATCAAGCAGGCTACAACTCTCCAGAGAGGATATAAAGGAGATTTTCAGAGAGCACGACATCGACGGAGATGGTCGATTGAGCATGAACGAGGTGATGAAGGCCTTCGGGTTCTTAGGTAGTATTCTTCCTTTCTACAAGGCTCACTATGGAATGGCTCATGCTGATGCTGATAACGATGGCTACATCGTCGAGGATGAGCTCGACAAGCTCGTTGACTATGCTGAGAAATTTCAACACAAAAGACAATGTTGA

mRNA sequence

ATGTCTCGTGCATCAAGCAGGCTACAACTCTCCAGAGAGGATATAAAGGAGATTTTCAGAGAGCACGACATCGACGGAGATGGTCGATTGAGCATGAACGAGGTGATGAAGGCCTTCGGGTTCTTAGGTAGTATTCTTCCTTTCTACAAGGCTCACTATGGAATGGCTCATGCTGATGCTGATAACGATGGCTACATCGTCGAGGATGAGCTCGACAAGCTCGTTGACTATGCTGAGAAATTTCAACACAAAAGACAATGTTGA

Coding sequence (CDS)

ATGTCTCGTGCATCAAGCAGGCTACAACTCTCCAGAGAGGATATAAAGGAGATTTTCAGAGAGCACGACATCGACGGAGATGGTCGATTGAGCATGAACGAGGTGATGAAGGCCTTCGGGTTCTTAGGTAGTATTCTTCCTTTCTACAAGGCTCACTATGGAATGGCTCATGCTGATGCTGATAACGATGGCTACATCGTCGAGGATGAGCTCGACAAGCTCGTTGACTATGCTGAGAAATTTCAACACAAAAGACAATGTTGA

Protein sequence

MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDGYIVEDELDKLVDYAEKFQHKRQC

Homology

[Close]

BLAST of ClCG08G015850 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.6e-35
Identity = 73/87 (83.91%), Postives = 82/87 (94.25%), Query Frame = 1


Query: 1  MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
          MSRA++RLQLSREDIKEIFREHDIDGDGRLSMNE++KAFG  GSILPFYKAHYGMAHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DNDGYIVEDELDKLVDYAEKFQHKRQC 88
          + DG+I E+ELDKLVDYA+KFQHKR+C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of ClCG08G015850 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-24
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 1


Query: 6  SRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDGY 65
          S+   SRE++KEIFREHDIDGDG LS++E++KA GFLG  +PFYKAHYGMA+ D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 IVEDELDKLVDYAEKFQHKRQ 87
          I EDELDKL+DYAE+FQ +R+
Sbjct: 67 ISEDELDKLIDYAERFQKRRR 87

[Close]

BLAST of ClCG08G015850 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.9e-20
Identity = 51/81 (62.96%), Postives = 64/81 (79.01%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           +S +Q SRE IKEIF+ HD D DG L++ EV KAF FLG+ILP YKA++GM +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  YIVEDELDKLVDYAEKFQHKR 86
            I +DEL+KLVDYA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of ClCG08G015850 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.4e-18
Identity = 50/85 (58.82%), Postives = 61/85 (71.76%), Query Frame = 1


Query: 1   MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
           + R SS + LSRE IKEIF+ HD + DG L+  E+ KAF FLGS+ PFYKA YGM +ADA
Sbjct: 87  LDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADA 146

Query: 61  DNDGYIVEDELDKLVDYAEKFQHKR 86
           + DG I E ELDKL+DYA K   K+
Sbjct: 147 NEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of ClCG08G015850 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 6.8e-18
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           +S +Q SRE IKEIF+ +D D DG L++ EV KAF  LGSI PF KA++GM +AD + DG
Sbjct: 120 ASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYADTNKDG 179

Query: 65  YIVEDELDKLVDYAEKFQHKR 86
            I EDELDKL++YA KF  K+
Sbjct: 180 LIGEDELDKLINYANKFMKKK 200

[Close]

BLAST of ClCG08G015850 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 156.4 bits (394), Expect = 2.3e-35
Identity = 73/87 (83.91%), Postives = 82/87 (94.25%), Query Frame = 1


Query: 1  MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
          MSRA++RLQLSREDIKEIFREHDIDGDGRLSMNE++KAFG  GSILPFYKAHYGMAHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DNDGYIVEDELDKLVDYAEKFQHKRQC 88
          + DG+I E+ELDKLVDYA+KFQHKR+C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of ClCG08G015850 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 150.6 bits (379), Expect = 1.3e-33
Identity = 70/83 (84.34%), Postives = 78/83 (93.98%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           S+RLQLSREDIKEIFREHDIDGDGRLSMNE++KAFG  GSILPFYKAHYGMAHAD + DG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 65  YIVEDELDKLVDYAEKFQHKRQC 88
           +I E+ELDKLVDYA+KFQHKR+C
Sbjct: 146 FIEEEELDKLVDYAQKFQHKRKC 168

[Close]

BLAST of ClCG08G015850 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 2.0e-15
Identity = 41/78 (52.56%), Postives = 57/78 (73.08%), Query Frame = 1


Query: 3  RASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADN 62
          +  S  +L +E++KE+FREHD +GDGRLS+ E+ +AFG  G+  P Y+A +G+  AD D 
Sbjct: 8  KKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDG 67

Query: 63 DGYIVEDELDKLVDYAEK 81
          DG+I E ELDK+VDYA K
Sbjct: 68 DGFISEQELDKVVDYAIK 85

HSP 2 Score: 120.2 bits (300), Expect = 1.8e-24
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 1


Query: 6  SRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDGY 65
          S+   SRE++KEIFREHDIDGDG LS++E++KA GFLG  +PFYKAHYGMA+ D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 IVEDELDKLVDYAEKFQHKRQ 87
          I EDELDKL+DYAE+FQ +R+
Sbjct: 67 ISEDELDKLIDYAERFQKRRR 87

[Close]

BLAST of ClCG08G015850 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 106.3 bits (264), Expect = 2.7e-20
Identity = 51/81 (62.96%), Postives = 64/81 (79.01%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           +S +Q SRE IKEIF+ HD D DG L++ EV KAF FLG+ILP YKA++GM +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  YIVEDELDKLVDYAEKFQHKR 86
            I +DEL+KLVDYA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of ClCG08G015850 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 100.1 bits (248), Expect = 2.0e-18
Identity = 50/85 (58.82%), Postives = 61/85 (71.76%), Query Frame = 1


Query: 1   MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
           + R SS + LSRE IKEIF+ HD + DG L+  E+ KAF FLGS+ PFYKA YGM +ADA
Sbjct: 87  LDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADA 146

Query: 61  DNDGYIVEDELDKLVDYAEKFQHKR 86
           + DG I E ELDKL+DYA K   K+
Sbjct: 147 NEDGLISEAELDKLIDYASKIIKKK 171

The following BLAST results are available for this feature:

BLAST of ClCG08G015850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC1_CUCSA	1.6e-35	83.91	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	1.3e-24	66.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	1.9e-20	62.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	1.4e-18	58.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]
A0A0A0KLF3_CUCSA	6.8e-18	58.02	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1	[more]

BLAST of ClCG08G015850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207397\|gb\|KGN62516.1\|	2.3e-35	83.91	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	1.3e-33	84.34	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	2.0e-15	52.56	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	2.7e-20	62.96	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|700194510\|gb\|KGN49687.1\|	2.0e-18	58.82	hypothetical protein Csa_5G067410 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG08G015850.1	ClCG08G015850.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 2..38 score: 2.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 55..81 score: 5.81coord: 10..45 score: 14
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 11..77 score: 3.8
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 6..73 score: 6.07
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..78 score: 6.4
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 5..78 score: 6.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
ClCG08G015850	Cucsa.232850	Cucumber (Gy14) v1	cgywcgB407
ClCG08G015850	MELO3C010866.2	Melon (DHL92) v3.6.1	medwcgB288

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG08G015850	Cucumber (Gy14) v1	cgywcgB195
ClCG08G015850	Cucumber (Gy14) v1	cgywcgB399
ClCG08G015850	Cucurbita maxima (Rimu)	cmawcgB029
ClCG08G015850	Cucurbita maxima (Rimu)	cmawcgB140
ClCG08G015850	Cucurbita maxima (Rimu)	cmawcgB169
ClCG08G015850	Cucurbita maxima (Rimu)	cmawcgB434
ClCG08G015850	Cucurbita maxima (Rimu)	cmawcgB712
ClCG08G015850	Cucurbita moschata (Rifu)	cmowcgB023
ClCG08G015850	Cucurbita moschata (Rifu)	cmowcgB123
ClCG08G015850	Cucurbita moschata (Rifu)	cmowcgB161
ClCG08G015850	Cucurbita moschata (Rifu)	cmowcgB435
ClCG08G015850	Cucurbita moschata (Rifu)	cmowcgB716
ClCG08G015850	Wild cucumber (PI 183967)	cpiwcgB172
ClCG08G015850	Wild cucumber (PI 183967)	cpiwcgB350
ClCG08G015850	Wild cucumber (PI 183967)	cpiwcgB601
ClCG08G015850	Cucumber (Chinese Long) v2	cuwcgB169
ClCG08G015850	Cucumber (Chinese Long) v2	cuwcgB332
ClCG08G015850	Cucumber (Chinese Long) v2	cuwcgB568
ClCG08G015850	Melon (DHL92) v3.5.1	mewcgB295
ClCG08G015850	Melon (DHL92) v3.5.1	mewcgB223
ClCG08G015850	Melon (DHL92) v3.5.1	mewcgB502
ClCG08G015850	Watermelon (97103) v1	wcgwmB400
ClCG08G015850	Watermelon (97103) v1	wcgwmB412
ClCG08G015850	Watermelon (97103) v1	wcgwmB421
ClCG08G015850	Cucurbita pepo (Zucchini)	cpewcgB125
ClCG08G015850	Cucurbita pepo (Zucchini)	cpewcgB528
ClCG08G015850	Cucurbita pepo (Zucchini)	cpewcgB624
ClCG08G015850	Cucurbita pepo (Zucchini)	cpewcgB708
ClCG08G015850	Cucurbita pepo (Zucchini)	cpewcgB748
ClCG08G015850	Bottle gourd (USVL1VR-Ls)	lsiwcgB305
ClCG08G015850	Bottle gourd (USVL1VR-Ls)	lsiwcgB438
ClCG08G015850	Cucumber (Gy14) v2	cgybwcgB155
ClCG08G015850	Cucumber (Gy14) v2	cgybwcgB309
ClCG08G015850	Cucumber (Gy14) v2	cgybwcgB529
ClCG08G015850	Melon (DHL92) v3.6.1	medwcgB214
ClCG08G015850	Melon (DHL92) v3.6.1	medwcgB496
ClCG08G015850	Silver-seed gourd	carwcgB0178
ClCG08G015850	Silver-seed gourd	carwcgB0752
ClCG08G015850	Cucumber (Chinese Long) v3	cucwcgB176
ClCG08G015850	Cucumber (Chinese Long) v3	cucwcgB350
ClCG08G015850	Watermelon (97103) v2	wcgwmbB327
ClCG08G015850	Watermelon (97103) v2	wcgwmbB336
ClCG08G015850	Wax gourd	wcgwgoB612
ClCG08G015850	Wax gourd	wcgwgoB656
ClCG08G015850	Watermelon (Charleston Gray)	wcgwcgB138
ClCG08G015850	Watermelon (Charleston Gray)	wcgwcgB166