ClCG08G004130 (gene) Watermelon (Charleston Gray)

NameClCG08G004130
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionrRNA N-glycosidase
LocationCG_Chr08 : 13029921 .. 13030844 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGAAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGTAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGTTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACGGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCGTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCGGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTCATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAGCCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGAGGAAACGGACAATTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAGGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATTACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAATAAATTCTGGCTACAAAAAGGAAACCAAATTGGTTCAGCTATCAATATGA

mRNA sequence

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGAAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGTAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGTTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACGGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCGTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCGGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTCATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAGCCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGAGGAAACGGACAATTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAGGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATTACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAATAAATTCTGGCTACAAAAAGGAAACCAAATTGGTTCAGCTATCAATATGA

Coding sequence (CDS)

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGAAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGTAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGTTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACGGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCGTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCGGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTCATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAGCCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGAGGAAACGGACAATTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAGGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATTACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAATAAATTCTGGCTACAAAAAGGAAACCAAATTGGTTCAGCTATCAATATGA

Protein sequence

MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAIMSPINSGYKKETKLVQLSI
BLAST of ClCG08G004130 vs. Swiss-Prot
Match: RIP1_TRIAN (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina GN=TCA PE=1 SV=2)

HSP 1 Score: 408.3 bits (1048), Expect = 7.4e-113
Identity = 206/284 (72.54%), Postives = 237/284 (83.45%), Query Frame = 1

Query: 5   IAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTA 64
           +A+ FF +AI +G PT  GDVSF L TAT+KSY+SFI QLR+ALPT G VC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKV 124
           SG QWF FF+LTNYNDET+TVAVNVTNVYIVAYRA AVSYFFEDT AEA+ L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAAR 184
           KLPYSGNYD+LQ+VVGKQRD IELG PALSSAITNM YYDY+ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTR 244
           +KYIEQQ+S +I+ NF P+ A+ISLEN WGALSKQIQIAN  G+GQFE+P++L  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            SVTNTSAGVVKGNIKLLL+Y      V  + D+ T++LHPGA+
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKA---SVGSEYDIPTTILHPGAM 281

BLAST of ClCG08G004130 vs. Swiss-Prot
Match: RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 PE=1 SV=2)

HSP 1 Score: 296.2 bits (757), Expect = 4.1e-79
Identity = 157/282 (55.67%), Postives = 203/282 (71.99%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

BLAST of ClCG08G004130 vs. Swiss-Prot
Match: RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.4e-79
Identity = 157/282 (55.67%), Postives = 202/282 (71.63%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F    LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

BLAST of ClCG08G004130 vs. Swiss-Prot
Match: RIP1_BRYDI (Ribosome-inactivating protein bryodin I OS=Bryonia dioica PE=1 SV=3)

HSP 1 Score: 285.8 bits (730), Expect = 5.6e-76
Identity = 162/276 (58.70%), Postives = 191/276 (69.20%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + ++  I+ IF+  PTV+GDVSF L  AT  SY  FI  LR ALP   KV NIP+L
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSA-EAWNLLFT 120
            S+ SG   +T   LTNY DETI+VAV+VTNVYI+ Y AG VSYFF + SA EA   +F 
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 DTRK-VKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQS 180
           D +K V LPYSGNY+RLQ   GK R+ I LG PAL SAIT ++YY     A+ALLVLIQS
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQS 180

Query: 181 TAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLI 240
           TAE+AR+K+IEQQI + ++  FLP LA ISLENNW ALSKQIQIA S  NGQFESP+ LI
Sbjct: 181 TAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVAD 275
             N  RVS+TN SA VV  NI LLL  N +A +  D
Sbjct: 241 DGNNQRVSITNASARVVTSNIALLLNRNNIAAIGED 275

BLAST of ClCG08G004130 vs. Swiss-Prot
Match: RIPT_TRIKI (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=1 SV=2)

HSP 1 Score: 281.2 bits (718), Expect = 1.4e-74
Identity = 157/283 (55.48%), Postives = 192/283 (67.84%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M++ + +   I+ +F+  P V+GDVSF L  AT  SY  FI  LR ALP   K+ +IP+L
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSA-EAWNLLFT 120
            S+  G Q +    LTNY DETI+VA++VTNVYI+ YRAG  SYFF + SA EA   +F 
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DT-RKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQS 180
           D  RKV LPYSGNY+RLQ   GK R+ I LG PAL SAIT +FYY+    A+AL+VLIQS
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLI 240
           T+EAAR+K+IEQQI + ++  FLP LAIISLEN+W ALSKQIQIA S  NGQFESP+ LI
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITS 282
                RV++TN  AGVV  NI LLL  N +A  + DD  M  S
Sbjct: 241 NAQNQRVTITNVDAGVVTSNIALLLNRNNMA-AMDDDVPMTQS 281

BLAST of ClCG08G004130 vs. TrEMBL
Match: Q0ZBB8_MOMCH (rRNA N-glycosidase OS=Momordica charantia GN=MAP30 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.3e-78
Identity = 158/282 (56.03%), Postives = 205/282 (72.70%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRHFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D D IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DEDFITTM 277

BLAST of ClCG08G004130 vs. TrEMBL
Match: W0LQW1_MOMCH (rRNA N-glycosidase OS=Momordica charantia PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 3.5e-77
Identity = 157/282 (55.67%), Postives = 205/282 (72.70%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLVLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIENFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVARYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

BLAST of ClCG08G004130 vs. TrEMBL
Match: Q5PZ05_MOMCH (rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.6e-77
Identity = 157/282 (55.67%), Postives = 205/282 (72.70%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

BLAST of ClCG08G004130 vs. TrEMBL
Match: S6CNA6_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.0e-76
Identity = 156/274 (56.93%), Postives = 201/274 (73.36%), Query Frame = 1

Query: 9   FFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTASGLQ 68
           F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L ST S  +
Sbjct: 3   FLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISDSR 62

Query: 69  WFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKVKLPY 128
            F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  TRK+ LPY
Sbjct: 63  RFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPY 122

Query: 129 SGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAARFKYI 188
           +GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +P  +ALLVLIQ+TAEAARFKYI
Sbjct: 123 TGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQPAPSALLVLIQTTAEAARFKYI 182

Query: 189 EQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTRVSVT 248
           E+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P G R  VT
Sbjct: 183 ERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQVT 242

Query: 249 NTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
           N  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 243 NVDSDVVKGNIKLLL--NSRASTA--DENFITTM 271

BLAST of ClCG08G004130 vs. TrEMBL
Match: S6CNA0_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.3e-76
Identity = 156/274 (56.93%), Postives = 201/274 (73.36%), Query Frame = 1

Query: 9   FFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTASGLQ 68
           F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L ST SG +
Sbjct: 3   FLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISGSR 62

Query: 69  WFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKVKLPY 128
            F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  TRK+ LPY
Sbjct: 63  RFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPY 122

Query: 129 SGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAARFKYI 188
           +GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TAEAARFKYI
Sbjct: 123 TGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFKYI 182

Query: 189 EQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTRVSVT 248
           E+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P G R  VT
Sbjct: 183 ERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQVT 242

Query: 249 NTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
           N  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 243 NVDSDVVKGNIKLLL--NSRASTA--DENFITTM 271

BLAST of ClCG08G004130 vs. NCBI nr
Match: gi|8247957|sp|P56626.2|RIP1_TRIAN (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-110
Identity = 206/284 (72.54%), Postives = 239/284 (84.15%), Query Frame = 1

Query: 5   IAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTA 64
           +A+ FF +AI +G PT  GDVSF L TAT+KSY+SFI QLR+ALPT G VC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKV 124
           SG QWF FF+LTNYNDET+TVAVNVTNVYIVAYRA AVSYFFEDT AEA+ L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAAR 184
           KLPYSGNYD+LQ+VVGKQRD IELG PALSSAITNM YYDY+ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTR 244
           +KYIEQQ+S +I+ NF P+ A+ISLEN WGALSKQIQIAN  G+GQFE+P++L  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            SVTNTSAGVVKGNIKLLL+Y      V  + D+ T++LHPGA+
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKA---SVGSEYDIPTTILHPGAM 281

BLAST of ClCG08G004130 vs. NCBI nr
Match: gi|109676416|gb|ABG37692.1| (anti-HIV protein [Momordica charantia])

HSP 1 Score: 298.5 bits (763), Expect = 1.3e-77
Identity = 158/282 (56.03%), Postives = 205/282 (72.70%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRHFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D D IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DEDFITTM 277

BLAST of ClCG08G004130 vs. NCBI nr
Match: gi|574452326|gb|AHG25320.1| (ribosome-inactivating protein [Momordica charantia])

HSP 1 Score: 296.6 bits (758), Expect = 5.0e-77
Identity = 157/282 (55.67%), Postives = 205/282 (72.70%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLVLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIENFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVARYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

BLAST of ClCG08G004130 vs. NCBI nr
Match: gi|46577684|sp|P24817.2|RIP3_MOMCH (RecName: Full=Ribosome-inactivating protein beta-momorcharin; Short=B-MMC; AltName: Full=MAP 30; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 296.2 bits (757), Expect = 6.6e-77
Identity = 157/282 (55.67%), Postives = 205/282 (72.70%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

BLAST of ClCG08G004130 vs. NCBI nr
Match: gi|132581|sp|P29339.1|RIP2_MOMBA (RecName: Full=Ribosome-inactivating protein momordin II; AltName: Full=rRNA N-glycosidase; Flags: Precursor)

HSP 1 Score: 295.8 bits (756), Expect = 8.6e-77
Identity = 157/282 (55.67%), Postives = 204/282 (72.34%), Query Frame = 1

Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F    LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RIP1_TRIAN7.4e-11372.54Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina GN=T... [more]
RIP3_MOMCH4.1e-7955.67Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia GN=MAP30 P... [more]
RIP2_MOMBA5.4e-7955.67Ribosome-inactivating protein momordin II OS=Momordica balsamina PE=3 SV=1[more]
RIP1_BRYDI5.6e-7658.70Ribosome-inactivating protein bryodin I OS=Bryonia dioica PE=1 SV=3[more]
RIPT_TRIKI1.4e-7455.48Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii PE=... [more]
Match NameE-valueIdentityDescription
Q0ZBB8_MOMCH9.3e-7856.03rRNA N-glycosidase OS=Momordica charantia GN=MAP30 PE=3 SV=1[more]
W0LQW1_MOMCH3.5e-7755.67rRNA N-glycosidase OS=Momordica charantia PE=2 SV=1[more]
Q5PZ05_MOMCH4.6e-7755.67rRNA N-glycosidase OS=Momordica charantia PE=3 SV=1[more]
S6CNA6_MOMCH1.0e-7656.93Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
S6CNA0_MOMCH1.3e-7656.93Ribosome inactivating protein (Fragment) OS=Momordica charantia GN=rip PE=4 SV=1[more]
Match NameE-valueIdentityDescription
gi|8247957|sp|P56626.2|RIP1_TRIAN1.2e-11072.54RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt... [more]
gi|109676416|gb|ABG37692.1|1.3e-7756.03anti-HIV protein [Momordica charantia][more]
gi|574452326|gb|AHG25320.1|5.0e-7755.67ribosome-inactivating protein [Momordica charantia][more]
gi|46577684|sp|P24817.2|RIP3_MOMCH6.6e-7755.67RecName: Full=Ribosome-inactivating protein beta-momorcharin; Short=B-MMC; AltNa... [more]
gi|132581|sp|P29339.1|RIP2_MOMBA8.6e-7755.67RecName: Full=Ribosome-inactivating protein momordin II; AltName: Full=rRNA N-gl... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001574Ribosome_inactivat_prot
IPR016138Ribosome_inactivat_prot_sub1
IPR016139Ribosome_inactivat_prot_sub2
IPR017988Ribosome_inactivat_prot_CS
IPR017989Ribosome_inactivat_1/2
Vocabulary: Biological Process
TermDefinition
GO:0017148negative regulation of translation
Vocabulary: Molecular Function
TermDefinition
GO:0030598rRNA N-glycosylase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0017148 negative regulation of translation
cellular_component GO:0005575 cellular_component
molecular_function GO:0030598 rRNA N-glycosylase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG08G004130.1ClCG08G004130.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 33..221
score: 3.0
IPR001574Ribosome-inactivating proteinunknownSSF56371Ribosome inactivating proteins (RIP)coord: 24..267
score: 1.44
IPR016138Ribosome-inactivating protein, subdomain 1GENE3DG3DSA:3.40.420.10coord: 24..183
score: 4.5
IPR016139Ribosome-inactivating protein, subdomain 2GENE3DG3DSA:4.10.470.10coord: 184..265
score: 2.3
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 176..192
scor
IPR017989Ribosome-inactivating protein subgroupPRINTSPR00396SHIGARICINcoord: 143..157
score: 5.6E-34coord: 35..48
score: 5.6E-34coord: 82..97
score: 5.6E-34coord: 172..192
score: 5.6E-34coord: 204..221
score: 5.6

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
ClCG08G004130MELO3C011620Melon (DHL92) v3.5.1mewcgB304
The following gene(s) are paralogous to this gene:

None