ClCG05G022060 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG05G022060
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	3-oxo-5-alpha-steroid 4-dehydrogenase
Location	CG_Chr05 : 34046116 .. 34046556 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTTAACGACGCGATTATCACCTCAGTTGCCTACCTACTCTTTACCGGCAGTACGATCTACGCTCAGATGGTTTCGCTGGATCTGCCGGAGCCGGAGATCGATCTGAAGGCGGCGGGATTGGCGCTGTACGGGATTGGAATCGCCGGTAATTTCTATCACCATATTCTACTGTCGAGATTGAGAAGAGAAGGCGATCGAGAGTACAGGATTCCGAGAGGAGGATTGTTTGAACTGATCGTGTGTCCTCACTACCTATTTGAGATTGTAGAATTTCTTGGAATCGCTTTGGTTTCGCAGACGTTGTTCGCGGTGGCGTTCGTGTTCGGATCGGCGTTGTTCTTGATTGGAAGAAGCTACGCCACCAGGAATTGGTACCTTTCCAAATTTGAAGATTTTCCTCGCCATGTTAAGGCCATTCTTCCGTTTGTTTTCTGA

mRNA sequence

ATGGCTCTTAACGACGCGATTATCACCTCAGTTGCCTACCTACTCTTTACCGGCAGTACGATCTACGCTCAGATGGTTTCGCTGGATCTGCCGGAGCCGGAGATCGATCTGAAGGCGGCGGGATTGGCGCTGTACGGGATTGGAATCGCCGGTAATTTCTATCACCATATTCTACTGTCGAGATTGAGAAGAGAAGGCGATCGAGAGTACAGGATTCCGAGAGGAGGATTGTTTGAACTGATCGTGTGTCCTCACTACCTATTTGAGATTGTAGAATTTCTTGGAATCGCTTTGGTTTCGCAGACGTTGTTCGCGGTGGCGTTCGTGTTCGGATCGGCGTTGTTCTTGATTGGAAGAAGCTACGCCACCAGGAATTGGTACCTTTCCAAATTTGAAGATTTTCCTCGCCATGTTAAGGCCATTCTTCCGTTTGTTTTCTGA

Coding sequence (CDS)

ATGGCTCTTAACGACGCGATTATCACCTCAGTTGCCTACCTACTCTTTACCGGCAGTACGATCTACGCTCAGATGGTTTCGCTGGATCTGCCGGAGCCGGAGATCGATCTGAAGGCGGCGGGATTGGCGCTGTACGGGATTGGAATCGCCGGTAATTTCTATCACCATATTCTACTGTCGAGATTGAGAAGAGAAGGCGATCGAGAGTACAGGATTCCGAGAGGAGGATTGTTTGAACTGATCGTGTGTCCTCACTACCTATTTGAGATTGTAGAATTTCTTGGAATCGCTTTGGTTTCGCAGACGTTGTTCGCGGTGGCGTTCGTGTTCGGATCGGCGTTGTTCTTGATTGGAAGAAGCTACGCCACCAGGAATTGGTACCTTTCCAAATTTGAAGATTTTCCTCGCCATGTTAAGGCCATTCTTCCGTTTGTTTTCTGA

Protein sequence

MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF

Homology

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BLAST of ClCG05G022060 vs. Swiss-Prot
Match: S5A1_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.3e-18
Identity = 45/106 (42.45%), Postives = 62/106 (58.49%), Query Frame = 1


Query: 41  GLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVS 100
           G  L+  G+  N +   +L  LR+ GD  Y+IPRGGLFE +   +Y  EI+E+ G AL S
Sbjct: 154 GFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALAS 213

Query: 101 QTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 147
            ++   AF F +  FL GR+     WYL KFE++P+  K I+PF+F
Sbjct: 214 WSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

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BLAST of ClCG05G022060 vs. Swiss-Prot
Match: S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.6e-18
Identity = 44/106 (41.51%), Postives = 62/106 (58.49%), Query Frame = 1


Query: 41  GLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVS 100
           G  L+  G+  N +   +L  LR+ GD  Y+IPRGGLFE +   +Y  EI+E+ G AL S
Sbjct: 158 GFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALAS 217

Query: 101 QTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 147
            ++   AF F +  FL GR+     WYL KFE++P+  K ++PF+F
Sbjct: 218 WSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

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BLAST of ClCG05G022060 vs. Swiss-Prot
Match: DET2_GOSHI (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum GN=DET2 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.8e-17
Identity = 44/108 (40.74%), Postives = 69/108 (63.89%), Query Frame = 1


Query: 40  AGLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALV 99
           AGL ++ +G+  N     +L  L+++GD  Y+IPRGGLFEL+ CP+Y  EI+E+ G A++
Sbjct: 151 AGLLIFVVGMWVNVRADKVLVGLKKQGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVM 210

Query: 100 SQTLFAVAFVFGSALFLIGRSYATRNWYLSKF-EDFPRHVKAILPFVF 147
           + +     F   +   L+ R+ ATR WYL KF +D+P+  KA++PF++
Sbjct: 211 TWSWVGFGFFLYTCANLMPRARATRLWYLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of ClCG05G022060 vs. Swiss-Prot
Match: S5A2_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 6.2e-17
Identity = 43/106 (40.57%), Postives = 68/106 (64.15%), Query Frame = 1


Query: 41  GLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVS 100
           G+ L+ +G+  N +   +L +LR+ G+  YRIP+GGLF  +   ++L EI+E++G AL +
Sbjct: 149 GVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEIIEWIGYALAT 208

Query: 101 QTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 147
            +L A+AF F S  FL  R++    +YL  FED+P+  KA++PF+F
Sbjct: 209 WSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of ClCG05G022060 vs. Swiss-Prot
Match: S5A2_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-16
Identity = 43/106 (40.57%), Postives = 68/106 (64.15%), Query Frame = 1


Query: 41  GLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVS 100
           G+ L+ +G+  N +   +L +LR+ G+  YRIP+GGLF  +   ++L EI+E++G AL +
Sbjct: 149 GVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALAT 208

Query: 101 QTLFAVAFVFGSALFLIGRSYATRNWYLSKFEDFPRHVKAILPFVF 147
            +L A+AF F S  FL  R++    +YL  FED+P+  KA++PF+F
Sbjct: 209 WSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

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BLAST of ClCG05G022060 vs. TrEMBL
Match: A0A0A0KUP2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612860 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 2.1e-64
Identity = 121/146 (82.88%), Postives = 135/146 (92.47%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           MAL DAI+TS++YLLF+G+TIYAQ+V+LDL EPEIDLKAAGLA+YGIGI GNFYHH+LLS
Sbjct: 111 MALTDAIMTSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLS 170

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           RLR  GDREY+IP+GGLF++IVCPHYLFEIVEFLGIA VSQTLF V FV GSALFLIGRS
Sbjct: 171 RLRSGGDREYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRS 230

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           YATR WYLSKFEDFP H+KAILPFVF
Sbjct: 231 YATRKWYLSKFEDFPSHIKAILPFVF 256

[Close]

BLAST of ClCG05G022060 vs. TrEMBL
Match: I1JDE1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.1e-47
Identity = 88/146 (60.27%), Postives = 116/146 (79.45%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           M L+ AI  +++Y L T + IYAQ ++  LPEP IDL   G+ L+ +GI GNFYHH LLS
Sbjct: 121 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPIDLLYPGIVLFVVGIIGNFYHHYLLS 180

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
            LR +G++EY+IP+GG+FEL++CPHYLFEI+EF G + +SQTL+A +F  G+ L+L+GRS
Sbjct: 181 NLRGKGEKEYKIPKGGMFELVICPHYLFEIIEFYGFSFISQTLYAFSFTVGTTLYLLGRS 240

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           Y+TR WYLSKFEDFP HVKAI+PFVF
Sbjct: 241 YSTRKWYLSKFEDFPEHVKAIIPFVF 266

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BLAST of ClCG05G022060 vs. TrEMBL
Match: A0A151RMQ6_CAJCA (Trans-2,3-enoyl-CoA reductase OS=Cajanus cajan GN=KK1_034676 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 4.0e-47
Identity = 87/146 (59.59%), Postives = 117/146 (80.14%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           M L+ AI  +++Y L T + IYAQ ++  LPEP I+L   G+AL+ +GI GN YHH LLS
Sbjct: 119 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPINLLYPGIALFVVGIIGNLYHHYLLS 178

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           +LR EG++EY+IP+GGLFEL++CPHYLFEI+ F G++ +SQTL+A +F  G+ L+L+GRS
Sbjct: 179 KLRGEGEKEYKIPKGGLFELVICPHYLFEIMVFYGVSFISQTLYAFSFTVGTTLYLLGRS 238

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           Y TR WYLSKFEDFP+HVKA++PFVF
Sbjct: 239 YVTRKWYLSKFEDFPKHVKAVIPFVF 264

[Close]

BLAST of ClCG05G022060 vs. TrEMBL
Match: A0A161X3V7_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006472 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 8.9e-47
Identity = 85/146 (58.22%), Postives = 118/146 (80.82%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           M ++  II S +Y LF  S IY+Q ++ +  EP IDLK AG A + +GI GNFYHH LLS
Sbjct: 126 MPIDTVIIISSSYFLFAVSVIYSQHLAEEFKEPIIDLKYAGTATFLVGITGNFYHHYLLS 185

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           +LR+EG+++Y+IP GGLF+L++CPHYLFEI+ F+GI+ +SQT++A++F FGS+ +L GRS
Sbjct: 186 KLRKEGEKQYKIPHGGLFDLVICPHYLFEIIGFIGISCISQTVYALSFTFGSSAYLTGRS 245

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           YATR WYLSKFEDFP+ +KA++P+VF
Sbjct: 246 YATRKWYLSKFEDFPKDIKALIPYVF 271

[Close]

BLAST of ClCG05G022060 vs. TrEMBL
Match: A0A166DSW4_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006471 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-46
Identity = 86/146 (58.90%), Postives = 117/146 (80.14%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           M L+  II S +Y LF  S IY+Q ++ +  EP IDLK AG A + +GI GNFYHH LLS
Sbjct: 123 MPLDTVIIISGSYFLFAVSVIYSQHLAAEFKEPIIDLKHAGTATFLVGITGNFYHHYLLS 182

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           +LR+EG+++Y+IP+GGLF L++CPHYLFEI  F+GI+ +SQT++A++F FGS+ +L GRS
Sbjct: 183 KLRKEGEKQYKIPQGGLFHLVICPHYLFEITGFIGISCISQTVYALSFTFGSSAYLTGRS 242

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           YATR WYLSKF+DFP+ +KA++PFVF
Sbjct: 243 YATRKWYLSKFKDFPKDIKALIPFVF 268

[Close]

BLAST of ClCG05G022060 vs. TAIR10
Match: AT5G16010.1 (AT5G16010.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 183.0 bits (463), Expect = 1.4e-46
Identity = 79/147 (53.74%), Postives = 113/147 (76.87%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           MA++ A+  S +Y   T   +Y+Q ++L L EP  D+K AG+ ++ +GI GN YHH+LL+
Sbjct: 122 MAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLA 181

Query: 61  RLRRE-GDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGR 120
           +LR+E G +EY+IP+GGLF++I+CPHYLFEI+ F    L+SQT+++ +F  G+ L+LIGR
Sbjct: 182 KLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGR 241

Query: 121 SYATRNWYLSKFEDFPRHVKAILPFVF 147
           SYATR WYLSKF+DFP+H+KA++PFVF
Sbjct: 242 SYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of ClCG05G022060 vs. TAIR10
Match: AT2G38050.1 (AT2G38050.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 77.4 bits (189), Expect = 8.1e-15
Identity = 39/107 (36.45%), Postives = 63/107 (58.88%), Query Frame = 1


Query: 41  GLALYGIGIAGNFYHHILLSRLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVS 100
           G+ ++  G+  N      L RL++E    Y IPRGG FEL+ CP+Y  E +E+LG A+++
Sbjct: 156 GMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMT 215

Query: 101 QTLFAVAFVFGSALFLIGRSYATRNWYLSKF-EDFPRHVKAILPFVF 147
            +   + F   +   L  R+ A+  WY++KF E++P+  KA++PFV+
Sbjct: 216 WSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of ClCG05G022060 vs. TAIR10
Match: AT3G55360.1 (AT3G55360.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 57.8 bits (138), Expect = 6.6e-09
Identity = 34/90 (37.78%), Postives = 46/90 (51.11%), Query Frame = 1


Query: 45  YGIGIA---GNFYHHILLSRLRR-EGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVS 104
           +G G+     NFY HILL  LR   G   Y+IPRG LF ++ C +Y  EI ++LG  + +
Sbjct: 200 FGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIAT 259

Query: 105 QTLFAVAFVFGSALFLIGRSYATRNWYLSK 131
           QT+    F+  +AL +        NW L K
Sbjct: 260 QTIAGYVFLAVAALIM-------TNWALGK 282

[Close]

BLAST of ClCG05G022060 vs. NCBI nr
Match: gi|659091767|ref|XP_008446721.1| (PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo])

HSP 1 Score: 262.7 bits (670), Expect = 3.8e-67
Identity = 130/146 (89.04%), Postives = 136/146 (93.15%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           MAL DAIITS+AYLLFTG+TIYAQ+VSLDL EPEIDLKAAGLALYGIGI GNFYHH+LLS
Sbjct: 116 MALTDAIITSLAYLLFTGATIYAQIVSLDLVEPEIDLKAAGLALYGIGITGNFYHHVLLS 175

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           RLR  GDREYRIPRGGLF++IVCPHYLFEIVEFLGIA VSQTLFAV FV GSALFLIGRS
Sbjct: 176 RLRSGGDREYRIPRGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFAVVFVVGSALFLIGRS 235

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           YATR WYLSKFEDFP HVKAILPFVF
Sbjct: 236 YATRKWYLSKFEDFPTHVKAILPFVF 261

[Close]

BLAST of ClCG05G022060 vs. NCBI nr
Match: gi|449435178|ref|XP_004135372.1| (PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis sativus])

HSP 1 Score: 253.1 bits (645), Expect = 3.0e-64
Identity = 121/146 (82.88%), Postives = 135/146 (92.47%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           MAL DAI+TS++YLLF+G+TIYAQ+V+LDL EPEIDLKAAGLA+YGIGI GNFYHH+LLS
Sbjct: 111 MALTDAIMTSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLS 170

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           RLR  GDREY+IP+GGLF++IVCPHYLFEIVEFLGIA VSQTLF V FV GSALFLIGRS
Sbjct: 171 RLRSGGDREYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRS 230

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           YATR WYLSKFEDFP H+KAILPFVF
Sbjct: 231 YATRKWYLSKFEDFPSHIKAILPFVF 256

[Close]

BLAST of ClCG05G022060 vs. NCBI nr
Match: gi|571439230|ref|XP_006574798.1| (PREDICTED: very-long-chain enoyl-CoA reductase-like [Glycine max])

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-47
Identity = 88/146 (60.27%), Postives = 116/146 (79.45%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           M L+ AI  +++Y L T + IYAQ ++  LPEP IDL   G+ L+ +GI GNFYHH LLS
Sbjct: 121 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPIDLLYPGIVLFVVGIIGNFYHHYLLS 180

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
            LR +G++EY+IP+GG+FEL++CPHYLFEI+EF G + +SQTL+A +F  G+ L+L+GRS
Sbjct: 181 NLRGKGEKEYKIPKGGMFELVICPHYLFEIIEFYGFSFISQTLYAFSFTVGTTLYLLGRS 240

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           Y+TR WYLSKFEDFP HVKAI+PFVF
Sbjct: 241 YSTRKWYLSKFEDFPEHVKAIIPFVF 266

[Close]

BLAST of ClCG05G022060 vs. NCBI nr
Match: gi|1012332425|gb|KYP43857.1| (Trans-2,3-enoyl-CoA reductase [Cajanus cajan])

HSP 1 Score: 195.7 bits (496), Expect = 5.8e-47
Identity = 87/146 (59.59%), Postives = 117/146 (80.14%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           M L+ AI  +++Y L T + IYAQ ++  LPEP I+L   G+AL+ +GI GN YHH LLS
Sbjct: 119 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPINLLYPGIALFVVGIIGNLYHHYLLS 178

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           +LR EG++EY+IP+GGLFEL++CPHYLFEI+ F G++ +SQTL+A +F  G+ L+L+GRS
Sbjct: 179 KLRGEGEKEYKIPKGGLFELVICPHYLFEIMVFYGVSFISQTLYAFSFTVGTTLYLLGRS 238

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           Y TR WYLSKFEDFP+HVKA++PFVF
Sbjct: 239 YVTRKWYLSKFEDFPKHVKAVIPFVF 264

[Close]

BLAST of ClCG05G022060 vs. NCBI nr
Match: gi|502178299|ref|XP_004516219.1| (PREDICTED: very-long-chain enoyl-CoA reductase-like [Cicer arietinum])

HSP 1 Score: 195.7 bits (496), Expect = 5.8e-47
Identity = 84/146 (57.53%), Postives = 118/146 (80.82%), Query Frame = 1


Query: 1   MALNDAIITSVAYLLFTGSTIYAQMVSLDLPEPEIDLKAAGLALYGIGIAGNFYHHILLS 60
           MAL+ AI  +++Y L   + IY Q ++LDLPEP+I+L   G+ L+ +G+ GNFYHH LLS
Sbjct: 126 MALDSAIPITLSYFLSAATMIYTQQLTLDLPEPQINLLYPGIVLFVVGVIGNFYHHYLLS 185

Query: 61  RLRREGDREYRIPRGGLFELIVCPHYLFEIVEFLGIALVSQTLFAVAFVFGSALFLIGRS 120
           +LR +G++EY+IP+GGLF  ++CPHYLFEI+EF G + +SQTL+A++F  G+  +L+GRS
Sbjct: 186 KLRGKGEKEYKIPKGGLFGFVICPHYLFEIIEFYGFSFISQTLYALSFTIGTTFYLLGRS 245

Query: 121 YATRNWYLSKFEDFPRHVKAILPFVF 147
           YATR WYLSKFEDFP++VKAI+PF+F
Sbjct: 246 YATRRWYLSKFEDFPKNVKAIIPFLF 271

The following BLAST results are available for this feature:

BLAST of ClCG05G022060 vs. Swiss-Prot


Match Name	E-value	Identity	Description
S5A1_HUMAN	7.3e-18	42.45	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1	[more]
S5A1_MACFA	9.6e-18	41.51	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV...	[more]
DET2_GOSHI	2.8e-17	40.74	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum GN=DET2 PE=1 SV=1	[more]
S5A2_MACFA	6.2e-17	40.57	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis GN=SRD5A2 PE=1 SV...	[more]
S5A2_HUMAN	1.1e-16	40.57	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens GN=SRD5A2 PE=1 SV=1	[more]

BLAST of ClCG05G022060 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUP2_CUCSA	2.1e-64	82.88	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612860 PE=4 SV=1	[more]
I1JDE1_SOYBN	1.1e-47	60.27	Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1	[more]
A0A151RMQ6_CAJCA	4.0e-47	59.59	Trans-2,3-enoyl-CoA reductase OS=Cajanus cajan GN=KK1_034676 PE=4 SV=1	[more]
A0A161X3V7_DAUCA	8.9e-47	58.22	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006472 PE=4 SV=1	[more]
A0A166DSW4_DAUCA	1.5e-46	58.90	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006471 PE=4 SV=1	[more]

BLAST of ClCG05G022060 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16010.1	1.4e-46	53.74	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	8.1e-15	36.45	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	6.6e-09	37.78	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of ClCG05G022060 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659091767\|ref\|XP_008446721.1\|	3.8e-67	89.04	PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo]	[more]
gi\|449435178\|ref\|XP_004135372.1\|	3.0e-64	82.88	PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis sativus]	[more]
gi\|571439230\|ref\|XP_006574798.1\|	1.5e-47	60.27	PREDICTED: very-long-chain enoyl-CoA reductase-like [Glycine max]	[more]
gi\|1012332425\|gb\|KYP43857.1\|	5.8e-47	59.59	Trans-2,3-enoyl-CoA reductase [Cajanus cajan]	[more]
gi\|502178299\|ref\|XP_004516219.1\|	5.8e-47	57.53	PREDICTED: very-long-chain enoyl-CoA reductase-like [Cicer arietinum]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001104	3-oxo-5_a-steroid_4-DH_C

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG05G022060.1	ClCG05G022060.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 37..146 score: 5.9
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROFILE	PS50244	S5A_REDUCTASE	coord: 41..110 score: 26
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 1..146 score: 2.1
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 1..146 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
ClCG05G022060	Cla020467	Watermelon (97103) v1	wcgwmB285

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG05G022060	Cucurbita pepo (Zucchini)	cpewcgB045
ClCG05G022060	Cucurbita pepo (Zucchini)	cpewcgB388
ClCG05G022060	Bottle gourd (USVL1VR-Ls)	lsiwcgB283
ClCG05G022060	Cucumber (Gy14) v2	cgybwcgB359
ClCG05G022060	Melon (DHL92) v3.6.1	medwcgB061
ClCG05G022060	Silver-seed gourd	carwcgB0816
ClCG05G022060	Silver-seed gourd	carwcgB1023
ClCG05G022060	Cucumber (Chinese Long) v3	cucwcgB415
ClCG05G022060	Watermelon (97103) v2	wcgwmbB229
ClCG05G022060	Wax gourd	wcgwgoB468
ClCG05G022060	Wax gourd	wcgwgoB469
ClCG05G022060	Cucumber (Gy14) v1	cgywcgB498
ClCG05G022060	Cucurbita maxima (Rimu)	cmawcgB364
ClCG05G022060	Cucurbita maxima (Rimu)	cmawcgB655
ClCG05G022060	Cucurbita moschata (Rifu)	cmowcgB358
ClCG05G022060	Cucurbita moschata (Rifu)	cmowcgB654
ClCG05G022060	Wild cucumber (PI 183967)	cpiwcgB412
ClCG05G022060	Cucumber (Chinese Long) v2	cuwcgB394
ClCG05G022060	Melon (DHL92) v3.5.1	mewcgB062