ClCG03G016540 (gene) Watermelon (Charleston Gray)

NameClCG03G016540
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionVesicle-associated membrane protein, putative
LocationCG_Chr03 : 31692475 .. 31697429 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCTTTTTCTTTTTCTTTTTCAATCACAATATTCCACAACTTTGTGGTATTCAATGGACTGTGTTTCTTACTCCTATGGCGTTCGAAATGATGACCCAATTCATCTACTAGTCCCCCACTTTCTTCTTTTCTTCCAACCTTTTGAAGCTCATCGGTTGAGTTCTGGTGACTGGAAAAATTCATTATGTGATCGCTATTACAATACTGTAATAAACATTTTCGGAAATTTCTTGTTGATTCTAATTTGTGAGTTTTTTTATTTTTTATTATTTATTTATACCTTAAACTCTGTAGAGATGTATTTCGAATATTATTATATTAGTGATTGTTTATTGTAGAAATCGTAGGGGTGTTTTTATAATCGATGGGTTCGAATAATTTAATTCGGTTCCTTGTTTGTATTTGGATGCATGTCTTCTGTTGTTTTGTTTATTCTGAGCTTCTATGGTTATGTTCGGCTTGTGAGTTTTCTTTTAATACACTTAATCCCCAAAAGTCTTTGCTTCAATTTTTCATGTTTTTTTTTTGTATTTCGATTGTGCAGGACAATTGTTTGATTAAATAGCCGAGTGAATTGCTTTTCAATCTAAGTTAAGTATGGATACGAGGCTTTTGGAAATTCATCCTCGGGAATTGAAGTTCATTTGTAAGTCTACATAATGAATTATTTTCTCTCTTTAGACTTTTGATTCGTTGTTCTCAATGGGCTGTTTACCGATTACCTCTGGAAAGATCAGGAAATTAGCTGCTGCTCTTGTTTCTTCCTATGAACCGTTTGATCTTTTGTGGCAAAAGCAAAGTGATGTTCTTGTTTGGGTGTTCATTTAGTGCTTTTTAGTTTTTGTAATTTATGTTTTAGTTCTTTCATTTGTACTTTGTTTTGTATGCTAATTGATCACAGCCGAAGGGTAAAAAAGTGCCTTTGCATAACTAAAAACATTTTTCAAATTTTGGTCGGTATTTGGTTGTGTATAGATAGGACAACATGGTTTTTATTTGAAAAACACTTAAAAGGTACTATTTGGAGAAGCGCTTAGATTTAGGTTTGAAATGTGTTTTGGACCTAATGATTTTAGGTGAAACACATTTTTATAAGCACGTTTGATAAACGAACTAATTAAGTGCTTACTTTTGAAGTATAATAAACAAACTTTTCAGTATTTAAAAAAATTTTAAAAGCACTTTTGACTTAATCAGTCGCCGGCAGTTTATAAAGCATTTTTTATAGTTAAAAGTACTTTTTACTCTTCTAAAAACGATATTAAACTCACGTTTTGTGCTTTTCCTGAAAGTGCTTATTAAAAATAGTGTTTTTTGAAACTTAAAAGTTATCTTAAAGCTGAAGTTTTGATGTATGATATTTTCTATGATGTCTGCTCTGGATTTAATTTCCTAAGCTACCTCAAACGAATTGTTGTATCTGCTTTTCTGAATATATTTTCTCATTTGCTACTAGGACATTGCTTACTTTAGACTTATTTATTATTATTTTTGTATGAACAAGGATATGTTATTTTCTACTTCATTTTATCATTCACTCCCTTTTCCAAATTAGAACTAATATCGTTCATATTTCCTTCTATGTCCTCCAGTTGAACTGAATAAACATAGCTTGTGCTCAATTCAACTAACAAATAATACGCTCGACCATGTTGCTTTTAAGGTATGGGTTACCAATCTTCCTCTCTGTCTCTGTCTCATTTTTTCTCTCTCTTGTACTTTTCAAAAAGCAAAAATCAATTGAACTGATTTATGATTCATCATGTAGAGATAGCATGTTATGTTGCATGCAAGATAATGGACAGTTTATTTTTTCAGGTTAAGATCACATCCCCCAAGAAGTACTATGTCCGGCCAAAGGTTGGAATTATCGAGCCAAACTCAACTCGTGAATTCACGGGTATTTACTTTTGACTAGTTAAGGCTTTACATTTTCTCTCTTCTATGGGTGGTATATTTATTTCTAGATGTTGATAAATTGAAGTCATGAGTGATCTTTTATCTTAAATAGCTTTAATGAGGCCTCATTGTGGCTACATTCTTCTGCTTCTAGCATCTTTGCTTGATTAGCTTTATCTTTATTTTTTCTATGCTTTCATTTCATGCCGTAGTCTATTTGGTGTACTTGCATCTTATACAGAAACTGTAAACCTATTTTTTTTTTTCAACTTAAGGGTACAGCCTGTCTTGTTTGACCTGTGTAGATTTATAAGATCTTTATGTTGGTCAGTAAGGGGAAATCACACTCTACAAGTTGGTTATGTAATGCAGAATAGGGAAGGAAAGGAAGAGGGGCCCACTAAGGAATGTGGCCTGTATATACGCCCGGGAGTGAGGGATGTTTCTTCCTGTGCTTTTTGTGAATTTGATGTTGCAAGAGAGGAGGGATGGAGAGATTACTCTTCCCTCTCTAATCGGCTGGGAGATTTTGTATCGCCTCAAGGCTTAACTTTCCTCTGTTTATCAATATATAGTAAGGGAGATACCTTACACAATGATTGGCTCAAGTGCTTGCACTAATCATGGGCTATTCTCTGTTTTGTCATATTGCTCTCATTCTTTCATTATCCCTTTCTCTCTTTCTTTTCATGTCCTGCACTTGCCTATAGCTTTTCCTGCTTTCCAAATTTTATATAATGGTTCTTAATGTAGTTAGGATGCAAGCACAAAAGCTGGCCCCACCTGATATGTTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGATATAACTTCTAGCTTGGTAAATGGTTCTACTACTATTTATTATGTATAATTGGCTATTTGTTTTGGAATTTAACGTTTTTCTTCTCATAAATTTCAGTTTAGCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCACCCATTTCCCAAGTGTTTTCCCCAGTTGATGAAGCAAAAAGGGAGGAGGAGGTGTTTCTTGGAGTTACAGAACCAAATAATCTATGTAAAGCAGTAGAGGAGATTGTAGAACAGGAACCTTTAAACAAAAACCATGTAATGTCTAATGGAGTTGATAGCCTGCCGCTAAAATACATGATTACAATTTCTCAATACATGCCTTTTTTGTATGCTTGCTTCAAAATACATTTACTTTTATCCCAAGTAGGATACTTAGATTAGCAATCATGGTAGAGTAGTTTTGAGAACGCCCTGGAAGCTTATAACATGGAAGCATCGAGACTTGGCACATGACTATAGCTAAATGTTTTGTTAAATTTTTTATGAAATGAAATGCATCTCTCTTCCAATCCAGCTATAACCATTTTTCTTTGCTTATTGAAATACAAAATTTTGGTTGTCCTGTTTATTGTCCCAGGTTCCCAGCAATGCTATGGAGCCTGAACCGGCAAGTTTAGCTAACGACTTCGTAGATGGCAACGGGGAGGACCTAGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAACTTGAGTTAAAGCTCTGTCAGGTCAGTCTTCCATAAAAATTGCTCCCATATTATGAACTCTGAGCATAATGATGAAGTGGAAAGATTTTCCAGAAACCTTATTTAAACCGTTAACTGAAGAAATTCTGCTAAACAGTGATGTGTATTGAAAAACTTTCTTTACAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTGTTGCAGGAGAATTTTGTAATTTCCTGTACCATTTTATTCTTTTTTCTTAAACGTATAATTCATATAACAATTAAAAGACCAATGCCATTTATTGTTGCCTATGTTATAACATCAAAGTACATCTGAAGACAGTTTATAGTTTCTGTTGGTCTGTTGAAGTCCAAATATAGTTTTTCCTGTCAACATCCTTAGATACCTGCATGAACTTTCCTCCAGTTTTCTTCAACTGAAATGAAGTCAGGGGTTACAAATTTAGCGAATGAACTTTGATAGTGATATCTAGTTGGTCCCTAAACCTTAAAAAGTTTTAATTACCCCTCTAAGACTTTGAATCATGATTTTATATAGTTAGTGTTTACACTGTTGATAAATAATAATGCAACATTGGTGGATAAATGTCGGCTGCTTTAGCAGAATATGGTAGACAAAAGATGAAAGGAGTGGATAATAGGAGAAAATAAGTGTCATAGTCTATTAAAGGGATTTGATGTTACATTGCTTCAACTAGATAGTACACTAAGCATATTACTTTATCTTCTCTAGTTGTGCACTTTTGTCTCAGTCATATTAATATTGAAACATTTAAAATTTAAAAGACTGAATTGAAGATTGAACTATGAAATACCATGGTAAAAAATCTGTATGTTTCGAGGACGGTTTGTTTCAGATTCTTGCTAAATTTGGACATCTGCTTAGCAATATGATTACATATTCTAGTTTTATAGTTGCAAATTTGTTGTACTATGATTTGTTTTGAATCTCAAATTCCTGAAGGCTGTGCATTTTGTAAATTTGCATTTTGCAGTCAGAGTTGAGGAAGACAGAGCTCAATAGCGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCACTTGTATGTACTTTTCTAGGACGCTTCTTGAGGTAGTAAAGATTTCTAGTGTCATTATATGTGTGTATCTTTTGCATATTGTCAAAGGAGGGGTTGGGATTGGTGTATAGCTTTGAAGTAAGAATAACTGTATAGGTTCTATTCTCCCTTATGACTCCCTCTCGGTTCGGATACCGCTTTCGTTGTGAATTTGAGCATAACTTAAGTAGTTCAATATTAGTGTCACTCCCAACTTGAGTATAGCACATTTGACAAGTTATCGACGTCTTCATAAGTCTAAATTGAGACACATCTTAAATGTGTTATAGTGTTAATGTTAGTATTACTATTTTAATGTAATTATCTTTGTCATAGTCGTT

mRNA sequence

TTTTCTTTTTCTTTTTCTTTTTCAATCACAATATTCCACAACTTTGTGGTATTCAATGGACTGTGTTTCTTACTCCTATGGCGTTCGAAATGATGACCCAATTCATCTACTAGTCCCCCACTTTCTTCTTTTCTTCCAACCTTTTGAAGCTCATCGGTTGAGTTCTGGTGACTGGAAAAATTCATTATGTGATCGCTATTACAATACTGTAATAAACATTTTCGGAAATTTCTTGTTGATTCTAATTTGACAATTGTTTGATTAAATAGCCGAGTGAATTGCTTTTCAATCTAAGTTAAGTATGGATACGAGGCTTTTGGAAATTCATCCTCGGGAATTGAAGTTCATTTTTGAACTGAATAAACATAGCTTGTGCTCAATTCAACTAACAAATAATACGCTCGACCATGTTGCTTTTAAGGTTAAGATCACATCCCCCAAGAAGTACTATGTCCGGCCAAAGGTTGGAATTATCGAGCCAAACTCAACTCGTGAATTCACGGTTAGGATGCAAGCACAAAAGCTGGCCCCACCTGATATGTTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGATATAACTTCTAGCTTGTTTAGCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCACCCATTTCCCAAGTGTTTTCCCCAGTTGATGAAGCAAAAAGGGAGGAGGAGGTGTTTCTTGGAGTTACAGAACCAAATAATCTATGTAAAGCAGTAGAGGAGATTGTAGAACAGGAACCTTTAAACAAAAACCATGTTCCCAGCAATGCTATGGAGCCTGAACCGGCAAGTTTAGCTAACGACTTCGTAGATGGCAACGGGGAGGACCTAGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAACTTGAGTTAAAGCTCTGTCAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTGTTGCAGGAGAATTTTTCAGAGTTGAGGAAGACAGAGCTCAATAGCGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCACTTGTATGTACTTTTCTAGGACGCTTCTTGAGGTAGTAAAGATTTCTAGTGTCATTATATGTGTGTATCTTTTGCATATTGTCAAAGGAGGGGTTGGGATTGGTGTATAGCTTTGAAGTAAGAATAACTGTATAGGTTCTATTCTCCCTTATGACTCCCTCTCGGTTCGGATACCGCTTTCGTTGTGAATTTGAGCATAACTTAAGTAGTTCAATATTAGTGTCACTCCCAACTTGAGTATAGCACATTTGACAAGTTATCGACGTCTTCATAAGTCTAAATTGAGACACATCTTAAATGTGTTATAGTGTTAATGTTAGTATTACTATTTTAATGTAATTATCTTTGTCATAGTCGTT

Coding sequence (CDS)

ATGGATACGAGGCTTTTGGAAATTCATCCTCGGGAATTGAAGTTCATTTTTGAACTGAATAAACATAGCTTGTGCTCAATTCAACTAACAAATAATACGCTCGACCATGTTGCTTTTAAGGTTAAGATCACATCCCCCAAGAAGTACTATGTCCGGCCAAAGGTTGGAATTATCGAGCCAAACTCAACTCGTGAATTCACGGTTAGGATGCAAGCACAAAAGCTGGCCCCACCTGATATGTTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGATATAACTTCTAGCTTGTTTAGCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCACCCATTTCCCAAGTGTTTTCCCCAGTTGATGAAGCAAAAAGGGAGGAGGAGGTGTTTCTTGGAGTTACAGAACCAAATAATCTATGTAAAGCAGTAGAGGAGATTGTAGAACAGGAACCTTTAAACAAAAACCATGTTCCCAGCAATGCTATGGAGCCTGAACCGGCAAGTTTAGCTAACGACTTCGTAGATGGCAACGGGGAGGACCTAGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAACTTGAGTTAAAGCTCTGTCAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTGTTGCAGGAGAATTTTTCAGAGTTGAGGAAGACAGAGCTCAATAGCGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCACTTGTATGTACTTTTCTAGGACGCTTCTTGAGGTAG

Protein sequence

MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPNSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLGVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR
BLAST of ClCG03G016540 vs. Swiss-Prot
Match: VAP22_ARATH (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana GN=PVA22 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 6.9e-41
Identity = 87/141 (61.70%), Postives = 102/141 (72.34%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+  LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            ST EFTV MQA K  PPDM+CKDKFLIQST V A+TTDEDIT+S+FSK  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKR 142
            VTLV P  S   SP++  K+
Sbjct: 121 RVTLVPPSDSPELSPINTPKQ 141


HSP 2 Score: 75.1 bits (183), Expect = 1.4e-12
Identity = 40/87 (45.98%), Postives = 56/87 (64.37%), Query Frame = 1

Query: 195 EDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLLQENFSELR-KTEL 254
           ++L+++K +EE+KLK+  LE KL QA + ISKL EE+ IS Q  + LQ   +ELR K  +
Sbjct: 299 DELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIV 358

Query: 255 NSVQVGFPVLYLYMVALVCTFLGRFLR 281
             V  GFP+LY+ +VA +   +G FLR
Sbjct: 359 KEVHNGFPLLYVCVVAFIAYVIGHFLR 385

BLAST of ClCG03G016540 vs. Swiss-Prot
Match: VAP11_ARATH (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana GN=PVA11 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.2e-32
Identity = 97/279 (34.77%), Postives = 127/279 (45.52%), Query Frame = 1

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           ++ LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G++ P 
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
           ST E  V MQAQK AP DM CKDKFL+Q  +     T +++T  +FSKE G  +EE KL 
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPE 181
           VT V+PP  +  SPV E                         E+    +  V  N    E
Sbjct: 139 VTYVAPP--RPPSPVHEGS-----------------------EEGSSPRASVSDNGHGSE 198

Query: 182 PASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLL 241
             S     VD      E       L  KL+E +    Q  N                  L
Sbjct: 199 -FSFERFIVDNKAGHQENTSEARALITKLTEEKQSAIQLNNR-----------------L 254

Query: 242 QENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           Q    +LR+    S   G P +Y+ +V L+   LG  ++
Sbjct: 259 QRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of ClCG03G016540 vs. Swiss-Prot
Match: VAP12_ARATH (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana GN=PVA12 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 72/142 (50.70%), Postives = 87/142 (61.27%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL I P +L+F FEL K   CS+ L N T ++VAFKVK T+PKKY VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S+ E  V MQAQK AP D+ CKDKFL+Q  V     T +D+T  +FSKE G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKRE 143
            V  V+PP  +  SPV E   E
Sbjct: 121 RVVYVAPP--RPPSPVREGSEE 140


HSP 2 Score: 41.2 bits (95), Expect = 2.2e-02
Identity = 19/62 (30.65%), Postives = 32/62 (51.61%), Query Frame = 1

Query: 219 QAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRF 278
           +A+  ++KL EEK  ++Q    LQ+   +LR+    S   G P +Y+ +V L+   LG  
Sbjct: 176 EARALVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYI 235

Query: 279 LR 281
           ++
Sbjct: 236 MK 237

BLAST of ClCG03G016540 vs. Swiss-Prot
Match: VAP14_ARATH (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana GN=PVA14 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-30
Identity = 90/242 (37.19%), Postives = 126/242 (52.07%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL I P +++F  ELNK   CS+ LTN T ++VAFK K T+ KKYYVRP VG++ P
Sbjct: 172 MSDELLIIDPVDVQFPIELNKKVSCSLNLTNKTENYVAFKAKTTNAKKYYVRPNVGVVLP 231

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S+ E  V MQA K AP DM C+DK L Q  VV  +TT +D+TS +FSKE G   EE +L
Sbjct: 232 RSSCEVLVIMQALKEAPADMQCRDKLLFQCKVVEPETTAKDVTSEMFSKEAGHPAEETRL 291

Query: 121 GVTLVSPPISQVFSPVDEAKRE-EEVFLGVTEPNNLCKAVEEIVEQ--EPLNKNHVPSNA 180
            V  V+PP  Q  SPV E   E       V++  N  +A  +++     PL  N   S  
Sbjct: 292 KVMYVTPP--QPPSPVQEGTEEGSSPRASVSDNGNASEAFVDMLRSLLVPLFSNAASSTD 351

Query: 181 MEPEPASLANDFVDGNGEDLE------MIK--HLEELKLKLSELELKLCQAQNAISKLKE 232
                      F++  G++L       ++K   LE++ +   E+EL+         +++E
Sbjct: 352 DHGITLPQYQVFINFRGDELRNSFVGFLVKAMRLEKINVFTDEVELRGTNLNYLFRRIEE 411


HSP 2 Score: 116.3 bits (290), Expect = 5.3e-25
Identity = 63/134 (47.01%), Postives = 80/134 (59.70%), Query Frame = 1

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPNSTR 64
           LL     +++F  ELNK   CS+ LTN T ++VAFK + T PK Y V+P VG++ P S+ 
Sbjct: 6   LLTFDHVDIRFPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPRSSC 65

Query: 65  EFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLGVTL 124
           E  V MQA K AP D  CKDK L Q  VV   T D+++TS +FSKE G  +EE    +  
Sbjct: 66  EVLVVMQALKEAPADRQCKDKLLFQCKVVEPGTMDKEVTSEMFSKEAGHRVEETIFKIIY 125

Query: 125 VSPPISQVFSPVDE 139
           V+PP  Q  SPV E
Sbjct: 126 VAPPQPQ--SPVQE 137

BLAST of ClCG03G016540 vs. Swiss-Prot
Match: VAP21_ARATH (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana GN=PVA21 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-30
Identity = 63/130 (48.46%), Postives = 89/130 (68.46%), Query Frame = 1

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           + +L+ I P ELKF+FEL K S C +++ N T ++VAFKVK TSPKKY+VRP  G+I+P 
Sbjct: 6   ENQLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPW 65

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
            +    V +QAQ+  PPDM CKDKFL+QST+VP  T  +++    F+K+ GK + E KL 
Sbjct: 66  DSCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLK 125

Query: 122 VTLVSPPISQ 132
           V+ ++P  +Q
Sbjct: 126 VSYITPSTTQ 135

BLAST of ClCG03G016540 vs. TrEMBL
Match: A0A0A0LZ53_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573020 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.3e-123
Identity = 236/288 (81.94%), Postives = 249/288 (86.46%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+TRLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKY+VRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LAPPDMLCKDKFLIQSTVVPADTTD DI SSLF+KEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN---- 180
           GV LV+P  S VFSP DEA            PNNLC+AVEEIV+QEPLNKNHVP N    
Sbjct: 121 GVILVTPS-SPVFSPADEA------------PNNLCEAVEEIVDQEPLNKNHVPDNNTEP 180

Query: 181 ----AMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEPAS ANDF+D N +++EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASSANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK LQENFSELRKTELN V+VGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 ISIQETKFLQENFSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of ClCG03G016540 vs. TrEMBL
Match: A0A067L7F4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02027 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.7e-60
Identity = 134/290 (46.21%), Postives = 178/290 (61.38%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LLEI PREL+F FEL K S CS+QL+N +  +VAFKVK TSPKKY VRP +G+I+P
Sbjct: 1   MAAELLEIQPRELRFTFELKKQSSCSVQLSNRSDQYVAFKVKTTSPKKYCVRPNIGVIKP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           N T EFTV MQAQK APPD  CKDKFL+Q  +VP  TTDEDITSS+FSKE GKY+EE+KL
Sbjct: 61  NVTSEFTVTMQAQKAAPPDFQCKDKFLVQGVIVPFGTTDEDITSSMFSKENGKYVEEKKL 120

Query: 121 GVTLVSPPISQVFSPVD-EAKR------------EEEVFLGVTEPNNLCKAVEEIVEQEP 180
            V L+SPP S V  P + E K+            E+     +  P N  + V      + 
Sbjct: 121 KVALISPPHSPVLLPNNGELKKDPCYDTSLHRDVEQNGIENIPPPQNSTEEVSSFQPAKN 180

Query: 181 LNKNHVPSNAMEPEPASLANDFVDG-NGEDLEMIKHLEELKLKLSELELKLCQAQNAISK 240
           + +     NA EP PA    +     +  + ++++  EELK KL  ++ KL +A+  I+K
Sbjct: 181 IEELKTVKNA-EPRPAEAIGNLESAKDAVEPKLLEDFEELKSKLHVMDSKLREAEQMITK 240

Query: 241 LKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLG 277
           L +E+  +I+E  LL+     LR   +  +QVGFP+LY+  VAL+C  LG
Sbjct: 241 LTDERSTAIKEKDLLKHEVKLLRSKNVKRIQVGFPLLYVCTVALICLALG 289

BLAST of ClCG03G016540 vs. TrEMBL
Match: A0A068VFA2_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00012958001 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.7e-60
Identity = 138/284 (48.59%), Postives = 182/284 (64.08%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL+I PRELKFI EL K S CS++L N +  +VAFKVK TSPKKY VRP  G+IEP
Sbjct: 1   MSNELLDIQPRELKFIVELKKQSSCSVRLFNKSKHYVAFKVKTTSPKKYCVRPNTGVIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S  +F V MQAQK+A PDM CKDKFL+Q TVV   TTD+DITSSLF+KE GKY+EE KL
Sbjct: 61  KSACDFLVTMQAQKVALPDMTCKDKFLVQGTVVAEGTTDDDITSSLFAKENGKYVEESKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHV--PSNAM 180
            V L SPP S V  P++    +  VF      + + +  E +  Q  + +N +   S  +
Sbjct: 121 RVVLASPPHSPVLLPINGVHDQVPVFETSPSKDQVLRGDENVGTQLKVIENMMEQKSENI 180

Query: 181 EPEPASLANDFVDGNG-EDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQE 240
           EP     A D     G E+LE+ K +E LK K+SELELKL +AQ  IS+L EE++++ +E
Sbjct: 181 EPPVTVEARDLERVKGVEELELAKDVENLKFKISELELKLEEAQVTISELTEERRLTARE 240

Query: 241 TKLLQENFSEL-RKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ++ L++  + L  K  +  VQVGFP L++ MV+LV   LG  +R
Sbjct: 241 SESLRQELALLSTKKGVRRVQVGFPFLFVCMVSLVSVVLGYLVR 284

BLAST of ClCG03G016540 vs. TrEMBL
Match: M0TSK2_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.7e-57
Identity = 132/294 (44.90%), Postives = 182/294 (61.90%), Query Frame = 1

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPNSTR 64
           L+EI P+ELKF FEL K S CSIQL N + D+VAFKVK TSPK+Y VRP +GI+ P ST 
Sbjct: 7   LVEIQPQELKFAFELKKQSSCSIQLENKSKDYVAFKVKTTSPKRYCVRPNIGILLPRSTC 66

Query: 65  EFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLGVTL 124
           +FTV MQA K APPDM  KDKFL+QSTVVP  TTDEDI  S FSKE G+YI+E KL V L
Sbjct: 67  DFTVTMQAPKEAPPDMQLKDKFLVQSTVVPYGTTDEDIVPSFFSKENGRYIQENKLRVVL 126

Query: 125 VSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN-------A 184
           VSPP S    P++ A R+E     + +    C  ++ + +QEP N+  +  N       +
Sbjct: 127 VSPPHSPALEPINVALRQEPA-NEIPDSAVTCIPIDGVSQQEPANEVPILRNISVSDNVS 186

Query: 185 MEPEPA---SLANDFVDGNGEDLEMI---------KHLEELKLKLSELELKLCQAQNAIS 244
           ++ EP    +++ D    N + L  +         K +++LKLKL+ +E+KL +A+  I 
Sbjct: 187 VKQEPVQEITISKDTSIPNEQALSGVAEITPSHVGKDIDDLKLKLNNIEVKLNEAEKTIM 246

Query: 245 KLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFL 280
            L++E   ++QE++ LQ+  + LRK     +Q GFP L++  +ALV   LG  L
Sbjct: 247 SLRKENSAAVQESEKLQQEIALLRKKSAVRIQAGFPFLFVVFLALVGMTLGYLL 299

BLAST of ClCG03G016540 vs. TrEMBL
Match: C6T767_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G252000 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.3e-57
Identity = 133/293 (45.39%), Postives = 176/293 (60.07%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M T LL+I P EL+F+FEL K S C +QL N T   +AFKVK TSPKKY VRP +GI++P
Sbjct: 1   MATELLQIEPAELRFVFELKKQSSCLVQLANTTDHFIAFKVKTTSPKKYCVRPNIGIVKP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           N   +FTV MQAQ++APPDMLCKDKFLIQSTVVP  TT++DITS +FSK+ GKYIEE+KL
Sbjct: 61  NDKCDFTVTMQAQRMAPPDMLCKDKFLIQSTVVPFGTTEDDITSDMFSKDSGKYIEEKKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREE---EVFL-------GVTEPNNLCKAVEEIVEQEPLN- 180
            V L+SPP S V  PV+   + +   E+++       GV      CK  +E+   EP   
Sbjct: 121 RVVLISPPSSPVLLPVNGDMKHDPSNEIYVQKDRVPSGVENIPPPCKVSDEVKGLEPAQD 180

Query: 181 -KNHVPSNAMEPEPASLANDFVDGNGE-DLEMIKHLEELKLKLSELELKLCQAQNAISKL 240
            K       + P  A    D      +  L +    EELK KLS ++ KL  A+  I+KL
Sbjct: 181 MKEDREDEDIVPRQAENVGDMKPAKDDMQLNLTNESEELKSKLSVMDSKLRVAEVTITKL 240

Query: 241 KEEKQISIQETKLLQENFSEL-RKTELNSVQVGFPVLYLYMVALVCTFLGRFL 280
            EE+  + QE  LL++    L R+      Q GFP L++ MVAL+   +G ++
Sbjct: 241 NEERHRNTQEKDLLKKELEVLKRQINTKGSQAGFPFLFVCMVALISVAVGYYI 293

BLAST of ClCG03G016540 vs. TAIR10
Match: AT1G08820.1 (AT1G08820.1 vamp/synaptobrevin-associated protein 27-2)

HSP 1 Score: 169.1 bits (427), Expect = 3.9e-42
Identity = 87/141 (61.70%), Postives = 102/141 (72.34%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+  LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            ST EFTV MQA K  PPDM+CKDKFLIQST V A+TTDEDIT+S+FSK  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKR 142
            VTLV P  S   SP++  K+
Sbjct: 121 RVTLVPPSDSPELSPINTPKQ 141


HSP 2 Score: 75.1 bits (183), Expect = 7.7e-14
Identity = 40/87 (45.98%), Postives = 56/87 (64.37%), Query Frame = 1

Query: 195 EDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLLQENFSELR-KTEL 254
           ++L+++K +EE+KLK+  LE KL QA + ISKL EE+ IS Q  + LQ   +ELR K  +
Sbjct: 299 DELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIV 358

Query: 255 NSVQVGFPVLYLYMVALVCTFLGRFLR 281
             V  GFP+LY+ +VA +   +G FLR
Sbjct: 359 KEVHNGFPLLYVCVVAFIAYVIGHFLR 385

BLAST of ClCG03G016540 vs. TAIR10
Match: AT3G60600.1 (AT3G60600.1 vesicle associated protein)

HSP 1 Score: 141.7 bits (356), Expect = 6.7e-34
Identity = 97/279 (34.77%), Postives = 127/279 (45.52%), Query Frame = 1

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           ++ LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G++ P 
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
           ST E  V MQAQK AP DM CKDKFL+Q  +     T +++T  +FSKE G  +EE KL 
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPE 181
           VT V+PP  +  SPV E                         E+    +  V  N    E
Sbjct: 139 VTYVAPP--RPPSPVHEGS-----------------------EEGSSPRASVSDNGHGSE 198

Query: 182 PASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLL 241
             S     VD      E       L  KL+E +    Q  N                  L
Sbjct: 199 -FSFERFIVDNKAGHQENTSEARALITKLTEEKQSAIQLNNR-----------------L 254

Query: 242 QENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           Q    +LR+    S   G P +Y+ +V L+   LG  ++
Sbjct: 259 QRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of ClCG03G016540 vs. TAIR10
Match: AT2G45140.1 (AT2G45140.1 plant VAP homolog 12)

HSP 1 Score: 135.2 bits (339), Expect = 6.3e-32
Identity = 72/142 (50.70%), Postives = 87/142 (61.27%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL I P +L+F FEL K   CS+ L N T ++VAFKVK T+PKKY VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S+ E  V MQAQK AP D+ CKDKFL+Q  V     T +D+T  +FSKE G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKRE 143
            V  V+PP  +  SPV E   E
Sbjct: 121 RVVYVAPP--RPPSPVREGSEE 140


HSP 2 Score: 41.2 bits (95), Expect = 1.2e-03
Identity = 19/62 (30.65%), Postives = 32/62 (51.61%), Query Frame = 1

Query: 219 QAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRF 278
           +A+  ++KL EEK  ++Q    LQ+   +LR+    S   G P +Y+ +V L+   LG  
Sbjct: 176 EARALVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYI 235

Query: 279 LR 281
           ++
Sbjct: 236 MK 237

BLAST of ClCG03G016540 vs. TAIR10
Match: AT1G51270.3 (AT1G51270.3 structural molecules;transmembrane receptors;structural molecules)

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-31
Identity = 90/242 (37.19%), Postives = 126/242 (52.07%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL I P +++F  ELNK   CS+ LTN T ++VAFK K T+ KKYYVRP VG++ P
Sbjct: 172 MSDELLIIDPVDVQFPIELNKKVSCSLNLTNKTENYVAFKAKTTNAKKYYVRPNVGVVLP 231

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S+ E  V MQA K AP DM C+DK L Q  VV  +TT +D+TS +FSKE G   EE +L
Sbjct: 232 RSSCEVLVIMQALKEAPADMQCRDKLLFQCKVVEPETTAKDVTSEMFSKEAGHPAEETRL 291

Query: 121 GVTLVSPPISQVFSPVDEAKRE-EEVFLGVTEPNNLCKAVEEIVEQ--EPLNKNHVPSNA 180
            V  V+PP  Q  SPV E   E       V++  N  +A  +++     PL  N   S  
Sbjct: 292 KVMYVTPP--QPPSPVQEGTEEGSSPRASVSDNGNASEAFVDMLRSLLVPLFSNAASSTD 351

Query: 181 MEPEPASLANDFVDGNGEDLE------MIK--HLEELKLKLSELELKLCQAQNAISKLKE 232
                      F++  G++L       ++K   LE++ +   E+EL+         +++E
Sbjct: 352 DHGITLPQYQVFINFRGDELRNSFVGFLVKAMRLEKINVFTDEVELRGTNLNYLFRRIEE 411


HSP 2 Score: 116.3 bits (290), Expect = 3.0e-26
Identity = 63/134 (47.01%), Postives = 80/134 (59.70%), Query Frame = 1

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPNSTR 64
           LL     +++F  ELNK   CS+ LTN T ++VAFK + T PK Y V+P VG++ P S+ 
Sbjct: 6   LLTFDHVDIRFPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPRSSC 65

Query: 65  EFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLGVTL 124
           E  V MQA K AP D  CKDK L Q  VV   T D+++TS +FSKE G  +EE    +  
Sbjct: 66  EVLVVMQALKEAPADRQCKDKLLFQCKVVEPGTMDKEVTSEMFSKEAGHRVEETIFKIIY 125

Query: 125 VSPPISQVFSPVDE 139
           V+PP  Q  SPV E
Sbjct: 126 VAPPQPQ--SPVQE 137

BLAST of ClCG03G016540 vs. TAIR10
Match: AT5G47180.1 (AT5G47180.1 Plant VAMP (vesicle-associated membrane protein) family protein)

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-31
Identity = 63/130 (48.46%), Postives = 89/130 (68.46%), Query Frame = 1

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           + +L+ I P ELKF+FEL K S C +++ N T ++VAFKVK TSPKKY+VRP  G+I+P 
Sbjct: 6   ENQLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPW 65

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
            +    V +QAQ+  PPDM CKDKFL+QST+VP  T  +++    F+K+ GK + E KL 
Sbjct: 66  DSCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLK 125

Query: 122 VTLVSPPISQ 132
           V+ ++P  +Q
Sbjct: 126 VSYITPSTTQ 135

BLAST of ClCG03G016540 vs. NCBI nr
Match: gi|778662640|ref|XP_011659931.1| (PREDICTED: vesicle-associated protein 2-1-like [Cucumis sativus])

HSP 1 Score: 449.9 bits (1156), Expect = 3.3e-123
Identity = 236/288 (81.94%), Postives = 249/288 (86.46%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+TRLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKY+VRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LAPPDMLCKDKFLIQSTVVPADTTD DI SSLF+KEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN---- 180
           GV LV+P  S VFSP DEA            PNNLC+AVEEIV+QEPLNKNHVP N    
Sbjct: 121 GVILVTPS-SPVFSPADEA------------PNNLCEAVEEIVDQEPLNKNHVPDNNTEP 180

Query: 181 ----AMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEPAS ANDF+D N +++EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASSANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK LQENFSELRKTELN V+VGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 ISIQETKFLQENFSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of ClCG03G016540 vs. NCBI nr
Match: gi|659099691|ref|XP_008450729.1| (PREDICTED: vesicle-associated protein 2-2-like [Cucumis melo])

HSP 1 Score: 448.7 bits (1153), Expect = 7.2e-123
Identity = 235/288 (81.60%), Postives = 247/288 (85.76%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+TRLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKY+VRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTR FTVRMQ Q+LAPPDMLCKDKFLIQSTVVPADTTD DI SSLF KEGGKYIEEQKL
Sbjct: 61  NSTRGFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFVKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN---- 180
           GV LV+P IS VF+PVDEA             NNLCKAVEEIV+QEPLNKNHVP N    
Sbjct: 121 GVILVTPSISPVFTPVDEAS------------NNLCKAVEEIVDQEPLNKNHVPDNKTEP 180

Query: 181 ----AMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEPASLAN+F+D N ++ EMIKHLEELKLKLSELELKLCQAQN+ISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASLANNFIDSNEDNAEMIKHLEELKLKLSELELKLCQAQNSISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK LQENFSELRK ELN VQVGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 ISIQETKFLQENFSELRKKELNRVQVGFPVLYLYMVALVCTFLGRLLR 276

BLAST of ClCG03G016540 vs. NCBI nr
Match: gi|802578214|ref|XP_012069416.1| (PREDICTED: vesicle-associated protein 2-2-like isoform X1 [Jatropha curcas])

HSP 1 Score: 240.4 bits (612), Expect = 3.9e-60
Identity = 134/290 (46.21%), Postives = 178/290 (61.38%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LLEI PREL+F FEL K S CS+QL+N +  +VAFKVK TSPKKY VRP +G+I+P
Sbjct: 1   MAAELLEIQPRELRFTFELKKQSSCSVQLSNRSDQYVAFKVKTTSPKKYCVRPNIGVIKP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           N T EFTV MQAQK APPD  CKDKFL+Q  +VP  TTDEDITSS+FSKE GKY+EE+KL
Sbjct: 61  NVTSEFTVTMQAQKAAPPDFQCKDKFLVQGVIVPFGTTDEDITSSMFSKENGKYVEEKKL 120

Query: 121 GVTLVSPPISQVFSPVD-EAKR------------EEEVFLGVTEPNNLCKAVEEIVEQEP 180
            V L+SPP S V  P + E K+            E+     +  P N  + V      + 
Sbjct: 121 KVALISPPHSPVLLPNNGELKKDPCYDTSLHRDVEQNGIENIPPPQNSTEEVSSFQPAKN 180

Query: 181 LNKNHVPSNAMEPEPASLANDFVDG-NGEDLEMIKHLEELKLKLSELELKLCQAQNAISK 240
           + +     NA EP PA    +     +  + ++++  EELK KL  ++ KL +A+  I+K
Sbjct: 181 IEELKTVKNA-EPRPAEAIGNLESAKDAVEPKLLEDFEELKSKLHVMDSKLREAEQMITK 240

Query: 241 LKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLG 277
           L +E+  +I+E  LL+     LR   +  +QVGFP+LY+  VAL+C  LG
Sbjct: 241 LTDERSTAIKEKDLLKHEVKLLRSKNVKRIQVGFPLLYVCTVALICLALG 289

BLAST of ClCG03G016540 vs. NCBI nr
Match: gi|661877819|emb|CDP18378.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 239.6 bits (610), Expect = 6.7e-60
Identity = 138/284 (48.59%), Postives = 182/284 (64.08%), Query Frame = 1

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL+I PRELKFI EL K S CS++L N +  +VAFKVK TSPKKY VRP  G+IEP
Sbjct: 1   MSNELLDIQPRELKFIVELKKQSSCSVRLFNKSKHYVAFKVKTTSPKKYCVRPNTGVIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S  +F V MQAQK+A PDM CKDKFL+Q TVV   TTD+DITSSLF+KE GKY+EE KL
Sbjct: 61  KSACDFLVTMQAQKVALPDMTCKDKFLVQGTVVAEGTTDDDITSSLFAKENGKYVEESKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHV--PSNAM 180
            V L SPP S V  P++    +  VF      + + +  E +  Q  + +N +   S  +
Sbjct: 121 RVVLASPPHSPVLLPINGVHDQVPVFETSPSKDQVLRGDENVGTQLKVIENMMEQKSENI 180

Query: 181 EPEPASLANDFVDGNG-EDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQE 240
           EP     A D     G E+LE+ K +E LK K+SELELKL +AQ  IS+L EE++++ +E
Sbjct: 181 EPPVTVEARDLERVKGVEELELAKDVENLKFKISELELKLEEAQVTISELTEERRLTARE 240

Query: 241 TKLLQENFSEL-RKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ++ L++  + L  K  +  VQVGFP L++ MV+LV   LG  +R
Sbjct: 241 SESLRQELALLSTKKGVRRVQVGFPFLFVCMVSLVSVVLGYLVR 284

BLAST of ClCG03G016540 vs. NCBI nr
Match: gi|743784708|ref|XP_010921728.1| (PREDICTED: vesicle-associated protein 1-2-like [Elaeis guineensis])

HSP 1 Score: 235.3 bits (599), Expect = 1.3e-58
Identity = 137/275 (49.82%), Postives = 172/275 (62.55%), Query Frame = 1

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPNSTR 64
           LLEI PRELKF FEL K S CSI L N + D+VAFKVK TSPK+Y VRP  G+I P ST 
Sbjct: 13  LLEIEPRELKFTFELKKQSSCSIHLVNKSSDYVAFKVKTTSPKRYCVRPNTGVILPRSTC 72

Query: 65  EFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLGVTL 124
           +FTV MQAQKLAPPDM  +DKFLIQ TVVP  TTDEDI  S FSKE G+YIEE KL V L
Sbjct: 73  DFTVTMQAQKLAPPDMQLRDKFLIQGTVVPYGTTDEDIVPSFFSKENGRYIEENKLRVVL 132

Query: 125 VSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPEPAS 184
           VSPP S V  P++ A ++E V+                  + P  K    SN  +PE   
Sbjct: 133 VSPPHSPVLQPINGALKQEPVY------------------EAPNLKGTPVSN--DPEVNE 192

Query: 185 LANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLLQEN 244
           + N  V        + K +E+LKLKL++LE+KL +A+N +++LKEE  ++IQE   LQ+ 
Sbjct: 193 VKNLPV------FHVSKEVEDLKLKLNDLEVKLNEAENTVTRLKEENGVAIQERDKLQQE 252

Query: 245 FSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFL 280
              LR+     VQVGFP+L++  + +V   LG  L
Sbjct: 253 IVLLRRKCTARVQVGFPILFVVFMTIVGVALGYLL 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
VAP22_ARATH6.9e-4161.70Vesicle-associated protein 2-2 OS=Arabidopsis thaliana GN=PVA22 PE=1 SV=1[more]
VAP11_ARATH1.2e-3234.77Vesicle-associated protein 1-1 OS=Arabidopsis thaliana GN=PVA11 PE=1 SV=1[more]
VAP12_ARATH1.1e-3050.70Vesicle-associated protein 1-2 OS=Arabidopsis thaliana GN=PVA12 PE=1 SV=1[more]
VAP14_ARATH1.9e-3037.19Vesicle-associated protein 1-4 OS=Arabidopsis thaliana GN=PVA14 PE=2 SV=1[more]
VAP21_ARATH1.9e-3048.46Vesicle-associated protein 2-1 OS=Arabidopsis thaliana GN=PVA21 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LZ53_CUCSA2.3e-12381.94Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573020 PE=4 SV=1[more]
A0A067L7F4_JATCU2.7e-6046.21Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02027 PE=4 SV=1[more]
A0A068VFA2_COFCA4.7e-6048.59Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00012958001 PE=4 SV=1[more]
M0TSK2_MUSAM3.7e-5744.90Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1[more]
C6T767_SOYBN6.3e-5745.39Uncharacterized protein OS=Glycine max GN=GLYMA_07G252000 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08820.13.9e-4261.70 vamp/synaptobrevin-associated protein 27-2[more]
AT3G60600.16.7e-3434.77 vesicle associated protein[more]
AT2G45140.16.3e-3250.70 plant VAP homolog 12[more]
AT1G51270.31.1e-3137.19 structural molecules;transmembrane receptors;structural molecules[more]
AT5G47180.11.1e-3148.46 Plant VAMP (vesicle-associated membrane protein) family protein[more]
Match NameE-valueIdentityDescription
gi|778662640|ref|XP_011659931.1|3.3e-12381.94PREDICTED: vesicle-associated protein 2-1-like [Cucumis sativus][more]
gi|659099691|ref|XP_008450729.1|7.2e-12381.60PREDICTED: vesicle-associated protein 2-2-like [Cucumis melo][more]
gi|802578214|ref|XP_012069416.1|3.9e-6046.21PREDICTED: vesicle-associated protein 2-2-like isoform X1 [Jatropha curcas][more]
gi|661877819|emb|CDP18378.1|6.7e-6048.59unnamed protein product [Coffea canephora][more]
gi|743784708|ref|XP_010921728.1|1.3e-5849.82PREDICTED: vesicle-associated protein 1-2-like [Elaeis guineensis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000535MSP_dom
IPR008962PapD-like_sf
IPR016763VAP
Vocabulary: Cellular Component
TermDefinition
GO:0005789endoplasmic reticulum membrane
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG03G016540.1ClCG03G016540.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..109
score: 1.9
IPR000535Major sperm protein (MSP) domainPROFILEPS50202MSPcoord: 5..125
score: 28
IPR008962PapD-likeGENE3DG3DSA:2.60.40.360coord: 3..122
score: 1.3
IPR008962PapD-likeunknownSSF49354PapD-likecoord: 3..132
score: 3.66
IPR016763Vesicle-associated membrane-protein-associated proteinPIRPIRSF019693VAMP_assoc_protcoord: 1..280
score: 2.8
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..128
score: 4.6E-101coord: 165..280
score: 4.6E
NoneNo IPR availableunknownCoilCoilcoord: 203..244
scor
NoneNo IPR availablePANTHERPTHR10809:SF59VESICLE-ASSOCIATED PROTEIN 2-2coord: 165..280
score: 4.6E-101coord: 1..128
score: 4.6E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
ClCG03G016540Csa1G573020Cucumber (Chinese Long) v2cuwcgB046
ClCG03G016540MELO3C015369Melon (DHL92) v3.5.1mewcgB245
ClCG03G016540Cla97C03G066850Watermelon (97103) v2wcgwmbB186
The following gene(s) are paralogous to this gene:

None