ClCG02G016010 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG02G016010
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	Kunitz-type trypsin inhibitor
Location	CG_Chr02 : 30406013 .. 30406934 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTTCAGACAAAATTAAATCTCTCTTCTTGAAGAACTAAAAGATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGTATATATTAAACTATAATACTAAACTCAATTGATTCATAAGTTATATCATATTTAATATTTTATATGTATTTGATATAACGTAAGCTGATAGATTATGATACATTTAATTCTCAACATTAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAAAGTGTGCAAATGTGTTCTAATTGCACTCATTTAAATTAAATAAAACCCCTGCAAGGATTTACTTTGTGTTCTTTAGTGTGTGTTATATTAATAATATGATTTGCC

mRNA sequence

ATTTTTCAGACAAAATTAAATCTCTCTTCTTGAAGAACTAAAAGATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAAAGTGTGCAAATGTGTTCTAATTGCACTCATTTAAATTAAATAAAACCCCTGCAAGGATTTACTTTGTGTTCTTTAGTGTGTGTTATATTAATAATATGATTTGCC

Coding sequence (CDS)

ATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAA

Protein sequence

MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK

Homology

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BLAST of ClCG02G016010 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 85.5 bits (210), Expect = 7.8e-16
Identity = 72/215 (33.49%), Postives = 100/215 (46.51%), Query Frame = 1


Query: 6   KSLAIFSCLCFFM---------PISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAG 65
           K L + S   FF+         P+ S A S   PVLD +G  L+    Y+I P + D  G
Sbjct: 2   KELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGG 61

Query: 66  NLTL--ITRKGD-QCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSI 125
            LT+   T  G   CP  V Q      D    + F P    ED +R   DL + F AF  
Sbjct: 62  GLTVSATTPNGTFVCPPRVVQ-TRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 121

Query: 126 C--ITSTEWKVGEADPTTGRRFVKLG------SDNTATGYFRIDK-SDLGVYNIGWCPSD 185
           C   +ST W++ + D +TG+ FV +G         T + +F+I++    G Y + +CP+ 
Sbjct: 122 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT- 181

Query: 186 VPIRGRPRCGSAGILI-EKGLRLLALDGSAFPFEF 199
           V    + +CG  GI I +KG R LAL    F FEF
Sbjct: 182 VCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEF 214

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BLAST of ClCG02G016010 vs. Swiss-Prot
Match: IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 8.1e-13
Identity = 47/141 (33.33%), Postives = 68/141 (48.23%), Query Frame = 1


Query: 17  FMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLP 76
           F P   +  +D PPV DT+G  L+ D  Y++  A     G LT+    G  CP +V Q P
Sbjct: 14  FWPAPPSRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP 73

Query: 77  LLSQDIGYGVTFRPY--LEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFV 136
               D G+ V   PY     +  IR   D+++ F+A++ C+ STEW + +++   GRR V
Sbjct: 74  NGQHD-GFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAAGRRHV 133

Query: 137 KLG-----SDNTATGYFRIDK 151
             G     S +     FRI+K
Sbjct: 134 ITGPVKDPSPSGRENAFRIEK 152

[Close]

BLAST of ClCG02G016010 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 8.9e-12
Identity = 61/178 (34.27%), Postives = 81/178 (45.51%), Query Frame = 1


Query: 7   SLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGD 66
           SL +   L   + IS +  +  PPV DTEG  L  D  Y++ PA     G LT+  R   
Sbjct: 3   SLRLPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRVLP 62

Query: 67  QCPFYVGQLPLLSQDIGYGVTFRPY---LEGED-TIREGRDLKVVFQAFSICITSTEWKV 126
            CP  V Q     +  G+ V F P+      ED TIR   D+++ F A +IC+ STEW V
Sbjct: 63  -CPLLVAQ-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHV 122

Query: 127 GEADPTTGRRFVKLG-----SDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGS 176
           G+ +P TG R V  G     S +     FR++K   G Y +  C       G  R G+
Sbjct: 123 GD-EPLTGARRVVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSCRDSCQDLGVSRDGA 176

[Close]

BLAST of ClCG02G016010 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.4e-11
Identity = 44/131 (33.59%), Postives = 66/131 (50.38%), Query Frame = 1


Query: 27  DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLSQDIGYGV 86
           D PPV DT+G  L+ D  Y++ PA     G LT+    G +CP +V Q     +D G  V
Sbjct: 1   DPPPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQEADGQRD-GLPV 60

Query: 87  TFRPY--LEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLG-----SD 146
              P+     +  IR   D+++ F+A++ C+ STEW + +++  +GRR V  G     S 
Sbjct: 61  RIAPHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHI-DSELVSGRRHVITGPVRDPSP 120

Query: 147 NTATGYFRIDK 151
           +     FRI+K
Sbjct: 121 SGRENAFRIEK 129

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BLAST of ClCG02G016010 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 9.2e-09
Identity = 45/149 (30.20%), Postives = 67/149 (44.97%), Query Frame = 1


Query: 24  AQSDGPPVLDTEGRPLQRDVEYFIKPAITDV-AGNLTLITRKGDQCPFYVGQLPLLSQDI 83
           A +   PVLDT+G  LQ  V+Y++  +I+    G L L    G  CP  V Q      D 
Sbjct: 24  ANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQ-RRSDLDN 83

Query: 84  GYGVTFRPYLEGEDTIREGRDLKVVFQAF--SICITSTEWKVGEADPTTGRRFVKLG--- 143
           G  V F      +D +R   D+ + F      +C TST W++   D + G+ +V      
Sbjct: 84  GTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVK 143

Query: 144 ---SDNTATGYFRIDKSDLGVYNIGWCPS 164
                NT   +F+I+K+ +  Y   +CPS
Sbjct: 144 GEPGPNTLCSWFKIEKAGVLGYKFRFCPS 171

[Close]

BLAST of ClCG02G016010 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 3.0e-99
Identity = 180/217 (82.95%), Postives = 198/217 (91.24%), Query Frame = 1


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM F+KSL A+FS LCFFM I STAQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTE 120
           LITRKGDQCPFYVGQ+PLLSQ+IG+GVT  PYLEG+DTIREGRDLK VFQAF+ICIT T+
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180
           WKVGE DP +GRRFVKLG+DNTATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           IEKG+R LALDG AFPFEFVRV+T  ++L SI ++KK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDT-GEELGSILKEKK 216

[Close]

BLAST of ClCG02G016010 vs. TrEMBL
Match: A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.3e-51
Identity = 107/183 (58.47%), Postives = 130/183 (71.04%), Query Frame = 1


Query: 18  MPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPL 77
           M I+STAQ   PPVLDT G+PLQR VEY+I PAITDV GNLTL +R    CP +VGQ P+
Sbjct: 1   MAITSTAQL--PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPV 60

Query: 78  LSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLG 137
            S +IG  VTFRP  EG D I EG  L +VFQA S C+TST+W+V   +  TGRRFV +G
Sbjct: 61  TSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIG 120

Query: 138 SDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 197
            ++   G F I + D G YNI WCP+   + GRPRCG AGIL+E G+RL+ALDG AFPFE
Sbjct: 121 DEDGPAGIFGISR-DNGAYNIVWCPA---MMGRPRCGRAGILVENGVRLVALDGDAFPFE 177

Query: 198 FVR 201
           F++
Sbjct: 181 FIK 177

[Close]

BLAST of ClCG02G016010 vs. TrEMBL
Match: D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.4e-50
Identity = 108/183 (59.02%), Postives = 123/183 (67.21%), Query Frame = 1


Query: 20  ISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLS 79
           ++  A    PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +CPFYVGQ PL S
Sbjct: 20  LAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQEPLAS 79

Query: 80  -QDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGS 139
            +  G  V F PY  GE  IRE RDL V FQA +ICI ST W+VGE DP TGRRF+    
Sbjct: 80  AEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRFIVTAG 139

Query: 140 DNTATGYFRIDKSDLGVYNIGWCPSD-VPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 199
           D +   YFRID +  GVYN  WCP++  P   RPRCGSAGILIE   RLLALDG AFPF 
Sbjct: 140 DKS---YFRIDNNG-GVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGPAFPFV 198

Query: 200 FVR 201
           F R
Sbjct: 200 FTR 198

[Close]

BLAST of ClCG02G016010 vs. TrEMBL
Match: G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.4e-50
Identity = 107/183 (58.47%), Postives = 123/183 (67.21%), Query Frame = 1


Query: 20  ISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLS 79
           ++  A    PPVLDT G+PL+  VEY+I P ITD+AG LTL+ R G +CPFYVGQ PL S
Sbjct: 20  LAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIRCPFYVGQEPLAS 79

Query: 80  -QDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGS 139
            +  G  V F PY  GE  IRE RDL V FQA +ICI ST W+VGE DP TGRRF+    
Sbjct: 80  AEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRFIVTAG 139

Query: 140 DNTATGYFRIDKSDLGVYNIGWCPSD-VPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 199
           D +   YFRID +  GVYN  WCP++  P   RPRCGSAGILIE   RLLALDG AFPF 
Sbjct: 140 DKS---YFRIDNNG-GVYNFSWCPTESCPTCDRPRCGSAGILIEDDKRLLALDGPAFPFV 198

Query: 200 FVR 201
           F R
Sbjct: 200 FTR 198

[Close]

BLAST of ClCG02G016010 vs. TrEMBL
Match: D1KFM4_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.4e-50
Identity = 108/183 (59.02%), Postives = 123/183 (67.21%), Query Frame = 1


Query: 20  ISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLS 79
           ++  A    PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +CPFYVGQ PL S
Sbjct: 20  LAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQEPLAS 79

Query: 80  -QDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGS 139
            +  G  V F PY  GE  IRE RDL V FQA +ICI ST W+VGE DP TGRRF+    
Sbjct: 80  AEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRFIVTAG 139

Query: 140 DNTATGYFRIDKSDLGVYNIGWCPSD-VPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 199
           D +   YFRID +  GVYN  WCP++  P   RPRCGSAGILIE   RLLALDG AFPF 
Sbjct: 140 DKS---YFRIDNNG-GVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGPAFPFV 198

Query: 200 FVR 201
           F R
Sbjct: 200 FTR 198

[Close]

BLAST of ClCG02G016010 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 369.4 bits (947), Expect = 4.3e-99
Identity = 180/217 (82.95%), Postives = 198/217 (91.24%), Query Frame = 1


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM F+KSL A+FS LCFFM I STAQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTE 120
           LITRKGDQCPFYVGQ+PLLSQ+IG+GVT  PYLEG+DTIREGRDLK VFQAF+ICIT T+
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180
           WKVGE DP +GRRFVKLG+DNTATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           IEKG+R LALDG AFPFEFVRV+T  ++L SI ++KK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDT-GEELGSILKEKK 216

[Close]

BLAST of ClCG02G016010 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 363.6 bits (932), Expect = 2.4e-97
Identity = 179/216 (82.87%), Postives = 192/216 (88.89%), Query Frame = 1


Query: 1   MMMFYKSLAIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MMM +KSLAIFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNLT
Sbjct: 1   MMMLHKSLAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTE 120
           LITRKGDQCPFYVGQ+PLLSQ+ G+ V+  PY EGEDTIREGRDLK VFQ F+ICIT T+
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 120

Query: 121 WKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180
           WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGIL
Sbjct: 121 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 180

Query: 181 IEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQK 216
           IEKG+R LALDG AFPFEFVRV+   ++L SI Q+K
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEK 215

[Close]

BLAST of ClCG02G016010 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-95
Identity = 178/217 (82.03%), Postives = 192/217 (88.48%), Query Frame = 1


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM F+KSL +IFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITST 120
           TLITRKGDQCPFYVGQ+PLLSQ+ G+ V+  PY EGEDTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180
           +WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQK 216
           LIEKG+R LALDG AFPFEFVRV+   ++L SI Q+K
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEK 216

[Close]

BLAST of ClCG02G016010 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-57
Identity = 118/200 (59.00%), Postives = 142/200 (71.00%), Query Frame = 1


Query: 1   MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 60
           MMM +KSLAIF   C FM I+STAQ   PPVLDT G+PLQR VEY+I PAITDV GNLTL
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQL--PPVLDTNGQPLQRGVEYYISPAITDVGGNLTL 60

Query: 61  ITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEW 120
            +R    CP +VGQ P+ S +IG  VTFRP  EG D I EG  L +VFQA S C+TST+W
Sbjct: 61  KSRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQW 120

Query: 121 KVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILI 180
           +V   +  TGRRFV +G ++   G F I + D G YNI WCP+   + GRPRCG AGIL+
Sbjct: 121 RVDATESDTGRRFVGIGDEDGPAGIFGISR-DNGAYNIVWCPA---MMGRPRCGRAGILV 180

Query: 181 EKGLRLLALDGSAFPFEFVR 201
           E G+RL+ALDG AFPFEF++
Sbjct: 181 ENGVRLVALDGDAFPFEFIK 194

[Close]

BLAST of ClCG02G016010 vs. NCBI nr
Match: gi|700205760|gb|KGN60879.1| (hypothetical protein Csa_2G021500 [Cucumis sativus])

HSP 1 Score: 210.3 bits (534), Expect = 3.3e-51
Identity = 107/183 (58.47%), Postives = 130/183 (71.04%), Query Frame = 1


Query: 18  MPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPL 77
           M I+STAQ   PPVLDT G+PLQR VEY+I PAITDV GNLTL +R    CP +VGQ P+
Sbjct: 1   MAITSTAQL--PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPV 60

Query: 78  LSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLG 137
            S +IG  VTFRP  EG D I EG  L +VFQA S C+TST+W+V   +  TGRRFV +G
Sbjct: 61  TSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIG 120

Query: 138 SDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 197
            ++   G F I + D G YNI WCP+   + GRPRCG AGIL+E G+RL+ALDG AFPFE
Sbjct: 121 DEDGPAGIFGISR-DNGAYNIVWCPA---MMGRPRCGRAGILVENGVRLVALDGDAFPFE 177

Query: 198 FVR 201
           F++
Sbjct: 181 FIK 177

The following BLAST results are available for this feature:

BLAST of ClCG02G016010 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MIRA_SYNDU	7.8e-16	33.49	Miraculin OS=Synsepalum dulcificum PE=1 SV=3	[more]
IAAS_HORVU	8.1e-13	33.33	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2	[more]
IAAS_ORYSJ	8.9e-12	34.27	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ...	[more]
IAAS_WHEAT	3.4e-11	33.59	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1	[more]
ASP_THECC	9.2e-09	30.20	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]

BLAST of ClCG02G016010 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4_CUCSA	3.0e-99	82.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1	[more]
A0A0A0LIK2_CUCSA	2.3e-51	58.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1	[more]
D1KFM3_9ROSI	3.4e-50	59.02	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides...	[more]
G0Z2L7_POPNI	3.4e-50	58.47	Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1	[more]
D1KFM4_9ROSI	3.4e-50	59.02	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=...	[more]

BLAST of ClCG02G016010 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442849\|ref\|XP_004139193.1\|	4.3e-99	82.95	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus]	[more]
gi\|659070428\|ref\|XP_008455022.1\|	2.4e-97	82.87	PREDICTED: miraculin-like isoform X2 [Cucumis melo]	[more]
gi\|659070426\|ref\|XP_008455014.1\|	1.3e-95	82.03	PREDICTED: miraculin-like isoform X1 [Cucumis melo]	[more]
gi\|449442847\|ref\|XP_004139192.1\|	1.1e-57	59.00	PREDICTED: miraculin-like [Cucumis sativus]	[more]
gi\|700205760\|gb\|KGN60879.1\|	3.3e-51	58.47	hypothetical protein Csa_2G021500 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG02G016010.1	ClCG02G016010.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..199 score: 2.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..202 score: 2.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 29..201 score: 9.16
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 29..203 score: 2.8
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..206 score: 1.0
None	No IPR available	PANTHER	PTHR33107:SF4	SUBFAMILY NOT NAMED	coord: 1..206 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG02G016010	Cucumber (Chinese Long) v3	cucwcgB137
ClCG02G016010	Watermelon (97103) v2	wcgwmbB138
ClCG02G016010	Wax gourd	wcgwgoB269
ClCG02G016010	Cucumber (Gy14) v1	cgywcgB103
ClCG02G016010	Cucurbita maxima (Rimu)	cmawcgB156
ClCG02G016010	Cucurbita maxima (Rimu)	cmawcgB695
ClCG02G016010	Cucurbita moschata (Rifu)	cmowcgB144
ClCG02G016010	Cucurbita moschata (Rifu)	cmowcgB700
ClCG02G016010	Wild cucumber (PI 183967)	cpiwcgB128
ClCG02G016010	Cucumber (Chinese Long) v2	cuwcgB122
ClCG02G016010	Melon (DHL92) v3.5.1	mewcgB375
ClCG02G016010	Watermelon (97103) v1	wcgwmB197
ClCG02G016010	Cucurbita pepo (Zucchini)	cpewcgB104
ClCG02G016010	Cucurbita pepo (Zucchini)	cpewcgB733
ClCG02G016010	Bottle gourd (USVL1VR-Ls)	lsiwcgB098
ClCG02G016010	Cucumber (Gy14) v2	cgybwcgB119
ClCG02G016010	Melon (DHL92) v3.6.1	medwcgB369
ClCG02G016010	Silver-seed gourd	carwcgB0692
ClCG02G016010	Silver-seed gourd	carwcgB0883