ClCG02G016000 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG02G016000
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	Kunitz trypsin inhibitor
Location	CG_Chr02 : 30401321 .. 30401908 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

mRNA sequence

ATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Coding sequence (CDS)

ATGATGATGCTGCATAAATCACTTGCCATCTTTGGCTATGCATGCCTCTTCATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Protein sequence

MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA

Homology

[Close]

BLAST of ClCG02G016000 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 95.5 bits (236), Expect = 6.8e-19
Identity = 74/211 (35.07%), Postives = 98/211 (46.45%), Query Frame = 1


Query: 7   SLAIFGYACLFMAI------TSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 66
           SL+ F  + L  A        + +   PV D DG+ LR G  YYI PV  D GG LT+  
Sbjct: 8   SLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVS- 67

Query: 67  RSNAPCPLFVGQEPVTSTNIGL----PVTFTPIMAGKDIIDEGTSLNIVFQALSTC--IT 126
            +  P   FV    V  T   +    P+ F P    +D++   T LNI F A   C   +
Sbjct: 68  -ATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTS 127

Query: 127 STQWRVDAAESDTGRRFVGIGREDGPAGIFGISR--------DNGVYNIVWCPAMMG--R 186
           ST WR+D  +  TG+ FV IG   G  G   IS          +G Y +V+CP + G  +
Sbjct: 128 STVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCK 187

Query: 187 PRCGRAGILV-ENGVRLLALDGEAFPFEFVK 195
            +CG  GI + + G R LAL  + F FEF K
Sbjct: 188 VKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of ClCG02G016000 vs. Swiss-Prot
Match: IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-13
Identity = 47/120 (39.17%), Postives = 61/120 (50.83%), Query Frame = 1


Query: 27  PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 86
           PPV DTDG  LR    YY+       GG LT+       CPLFV Q+P    + G PV  
Sbjct: 26  PPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFPVRI 85

Query: 87  TP--IMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG--REDGPAG 143
           TP  +     II   T + I F+A +TC+ ST+W +D +E   GRR V  G  ++  P+G
Sbjct: 86  TPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPSPSG 143

[Close]

BLAST of ClCG02G016000 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.5e-13
Identity = 49/120 (40.83%), Postives = 59/120 (49.17%), Query Frame = 1


Query: 27  PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 86
           PPV DTDG  LR    YY+ P     GG LT+       CPLFV QE       GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQE-ADGQRDGLPVRI 62

Query: 87  TPI--MAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG--REDGPAG 143
            P        II   T + I F+A +TC+ ST+W +D +E  +GRR V  G  R+  P+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHID-SELVSGRRHVITGPVRDPSPSG 120

[Close]

BLAST of ClCG02G016000 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 3.6e-12
Identity = 57/165 (34.55%), Postives = 75/165 (45.45%), Query Frame = 1


Query: 3   MLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRS 62
           M+   L +   + L ++ +  A  PPV+DT+G  L     YY+ P     GG LT+  R 
Sbjct: 1   MVSLRLPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFTP---IMAGKD-IIDEGTSLNIVFQALSTCITSTQW 122
             PCPL V QE       G PV FTP     A +D  I   T + I F A + C+ ST+W
Sbjct: 61  -LPCPLLVAQE-TDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEW 120

Query: 123 RVDAAESDTGRRFVGIGREDGPA-----GIFGISRDNGVYNIVWC 159
            V   E  TG R V  G   GP+       F + +  G Y +V C
Sbjct: 121 HV-GDEPLTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLVSC 162

[Close]

BLAST of ClCG02G016000 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 7.5e-10
Identity = 49/151 (32.45%), Postives = 68/151 (45.03%), Query Frame = 1


Query: 28  PVFDTDGQPLRRGVEYYI-SPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 87
           PV DTDG  L+ GV+YY+ S +    GG L L   +   CP  V Q      N G PV F
Sbjct: 30  PVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDLDN-GTPVIF 89

Query: 88  TPIMAGKDIIDEGTSLNIVFQAL--STCITSTQWRVDAAESDTGRRFV---GIGREDGP- 147
           +   +  D++   T +NI F  +    C TST WR+D  ++  G+ +V   G+  E GP 
Sbjct: 90  SNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKGEPGPN 149

Query: 148 ----------AGIFGISRDNGVYNIVWCPAM 162
                     AG+ G       Y   +CP++
Sbjct: 150 TLCSWFKIEKAGVLG-------YKFRFCPSV 172

[Close]

BLAST of ClCG02G016000 vs. TrEMBL
Match: A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 1.8e-87
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 1


Query: 18  MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTS 77
           MAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGRE 137
           TNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRVDA ESDTGRRFVGIG E
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA 196
           DGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]

BLAST of ClCG02G016000 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-55
Identity = 114/201 (56.72%), Postives = 135/201 (67.16%), Query Frame = 1


Query: 1   MMMLHKSL-AIFGYACLFMAITSIAQF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNLT 60
           MM  HKSL A+F + C FMAI S AQF  PPV DT+G+PL+R VEY+I P  TDV GNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQ 120
           L +R    CP +VGQ P+ S  IG  VT TP + G D I EG  L  VFQA + CIT TQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WRVDAAESDTGRRFVGIGREDGPAGIFGISRDN-GVYNIVWCPA---MMGRPRCGRAGIL 180
           W+V   +  +GRRFV +G ++   G F I + N GVYNI WCP+     GRPRCG AGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 VENGVRLLALDGEAFPFEFVK 195
           +E GVR LALDG AFPFEFV+
Sbjct: 181 IEKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of ClCG02G016000 vs. TrEMBL
Match: B9SIQ2_RICCO (Alpha-amylase/subtilisin inhibitor, putative OS=Ricinus communis GN=RCOM_0538680 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.3e-44
Identity = 94/189 (49.74%), Postives = 123/189 (65.08%), Query Frame = 1


Query: 12  GYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVG 71
           G ACL MA+++IAQ P V D+ GQPL  GVEYY+ P  TD  G LTL +R+ + CP +VG
Sbjct: 10  GLACLLMAVSTIAQTPAVLDSSGQPLTSGVEYYVLPAATDTAGGLTLVNRTGS-CPFYVG 69

Query: 72  QEPV-TSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRR 131
           QEP+ T    G PV FTP+ AG+ II EG    + F A++ C+ ST W +   +++T RR
Sbjct: 70  QEPLPTVAKTGFPVIFTPLAAGESIIREGMDFRVAFSAVTNCVQSTTWSIGDEDAETSRR 129

Query: 132 FVGIGREDGPAGIFGISRDNGVYNIVWCPAMMG----RPRCGRAGILVENGVRLLALDGE 191
           F+  G E+     F I ++ G+YN+ WCP        RPRCG AGIL+ENG RLLALDG 
Sbjct: 130 FIVTGGEE---DYFRIDKNEGLYNLGWCPNCNSINCPRPRCGFAGILIENGNRLLALDGA 189

Query: 192 AFPFEFVKA 196
           AFPF+F +A
Sbjct: 190 AFPFQFRRA 194

[Close]

BLAST of ClCG02G016000 vs. TrEMBL
Match: A0A0X7YK90_HEVBR (Alpha-amylase/subtilisin inhibitor OS=Hevea brasiliensis GN=ASI PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.0e-42
Identity = 98/198 (49.49%), Postives = 125/198 (63.13%), Query Frame = 1


Query: 6   KSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAP 65
           K +    +  L MA++++AQ P V DT+GQPLR GVEYY+ P  TD  G LTL +R+++ 
Sbjct: 3   KLIGSLSFVWLLMAMSTVAQTPAVLDTNGQPLRSGVEYYVLPAATDTAGGLTLVNRTDS- 62

Query: 66  CPLFVGQEPV-TSTNIGLPVTFTPIMAGKD-IIDEGTSLNIVFQALSTCITSTQWRVDAA 125
           CPL+VGQEP+ T  + GL VTFTP  A  D II E    ++ F A+S C  ST WRV   
Sbjct: 63  CPLYVGQEPLSTVVSQGLRVTFTPFAAQNDGIIRESREFSVAFSAVSICGQSTAWRVGEE 122

Query: 126 ESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPA------MMGRPRCGRAGILVENG 185
           +++T RRF+  G   G    F I  + G+Y +VWCP         GRPRCG AGIL+ENG
Sbjct: 123 DAETSRRFIVTG---GEQSYFRIDNNGGLYELVWCPGESCTAPNCGRPRCGSAGILIENG 182

Query: 186 VRLLALDGEAFPFEFVKA 196
            RLLALDG AFPF F +A
Sbjct: 183 KRLLALDGSAFPFRFRRA 196

[Close]

BLAST of ClCG02G016000 vs. TrEMBL
Match: B9N9S5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0019s02570g PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 6.8e-42
Identity = 99/203 (48.77%), Postives = 123/203 (60.59%), Query Frame = 1


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           M+ L +SL+   + C  MAI+S+AQ PPV DTDG PL RGVEYY+ P  TDV G LTL +
Sbjct: 1   MLRLIRSLS---FICQLMAISSMAQGPPVLDTDGNPLTRGVEYYVDPAITDVAGGLTLVA 60

Query: 61  RSNAPCPLFVGQEPVTSTNI-GLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWR 120
           R N  CP +VGQ P+   ++  LPV FTP   G  II E T   + F A STC+ +T W 
Sbjct: 61  R-NGSCPSYVGQVPIGPGSVEDLPVIFTPSNPGDTIITETTEFTVAFSASSTCVQNTTWG 120

Query: 121 VDAAESDTGRRFVGIGREDGPAGIFGISRDN--GVYNIVWCPA-----MMGRPRCGRAGI 180
           +   + +T +RF+ IG   G   +F I  D   G Y I WCPA     + GRPRCG AGI
Sbjct: 121 IGEEDPETRKRFIVIG---GEPTVFDIDSDQAVGPYTIGWCPACLNPPLCGRPRCGLAGI 180

Query: 181 LVENGVRLLALDGEAFPFEFVKA 196
           L +NG R L LDG AFPF F +A
Sbjct: 181 LEQNGTRFLTLDGPAFPFRFRRA 196

[Close]

BLAST of ClCG02G016000 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 66.6 bits (161), Expect = 1.9e-11
Identity = 59/199 (29.65%), Postives = 84/199 (42.21%), Query Frame = 1


Query: 8   LAIFGYACLFMA---ITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNA 67
           L IF    +F++   +T+ A   PV D +G+ L  GV YYI PV    GG LT+ +    
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 68  PCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAE 127
            CP  V Q+     + GLPV F+P    +  I   T +NI F   S       W +   +
Sbjct: 65  TCPTSVIQDQF-EVSQGLPVKFSPYDKSR-TIPVSTDVNIKFSPTSI------WELANFD 124

Query: 128 SDTGRRFVGIGREDGPAG------IFGISRDNGVYNIVWCPAMMG--RPRCGRAGILVEN 187
             T + F+     +G  G       F I +    Y I +CP +    +  C   G+ V++
Sbjct: 125 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 184

Query: 188 GVRLLALDGEAFPFEFVKA 196
           G R LAL        F +A
Sbjct: 185 GKRRLALSDVPLKVMFKRA 195

[Close]

BLAST of ClCG02G016000 vs. TAIR10
Match: AT1G73325.1 (AT1G73325.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 66.6 bits (161), Expect = 1.9e-11
Identity = 53/174 (30.46%), Postives = 82/174 (47.13%), Query Frame = 1


Query: 29  VFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTN---IGLPVT 88
           V D  G P++  V+YYI P     GG L   +R+ +   L +  + V S++    GLPVT
Sbjct: 32  VLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSSSPFVSGLPVT 91

Query: 89  FTPIMAGKDIIDEGTSLNIVFQA-LSTCITSTQWRVDAAESDTGRRFVGIGREDGPAGI- 148
           F+P+      +    SLN+ F + +  C  S  WR+D +     + FV IG + G     
Sbjct: 92  FSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHS-VQLRKSFVSIGGQKGKGNSW 151

Query: 149 FGISRDNGVYNIVWCPAMMGRPRCGRAGILV-ENGVRLLALD-GEAFPFEFVKA 196
           F I  D   Y +++CP +     C    + + ++GVR L L   ++F  +F KA
Sbjct: 152 FQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRRLVLSTDQSFVVKFQKA 203

[Close]

BLAST of ClCG02G016000 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 364.8 bits (935), Expect = 9.6e-98
Identity = 176/195 (90.26%), Postives = 182/195 (93.33%), Query Frame = 1


Query: 1   MMMLHKSLAIFGYACLFMAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKS 60
           MMMLHKSLAIFGYACLFMAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120

Query: 121 DAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DA ESDTGRRFVGIG EDGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGEAFPFEFVKA 196
           +ALDG+AFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of ClCG02G016000 vs. NCBI nr
Match: gi|700205760|gb|KGN60879.1| (hypothetical protein Csa_2G021500 [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 2.6e-87
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 1


Query: 18  MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTS 77
           MAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGRE 137
           TNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRVDA ESDTGRRFVGIG E
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA 196
           DGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]

BLAST of ClCG02G016000 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 222.2 bits (565), Expect = 7.7e-55
Identity = 114/201 (56.72%), Postives = 135/201 (67.16%), Query Frame = 1


Query: 1   MMMLHKSL-AIFGYACLFMAITSIAQF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNLT 60
           MM  HKSL A+F + C FMAI S AQF  PPV DT+G+PL+R VEY+I P  TDV GNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQ 120
           L +R    CP +VGQ P+ S  IG  VT TP + G D I EG  L  VFQA + CIT TQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WRVDAAESDTGRRFVGIGREDGPAGIFGISRDN-GVYNIVWCPA---MMGRPRCGRAGIL 180
           W+V   +  +GRRFV +G ++   G F I + N GVYNI WCP+     GRPRCG AGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 VENGVRLLALDGEAFPFEFVK 195
           +E GVR LALDG AFPFEFV+
Sbjct: 181 IEKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of ClCG02G016000 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 219.2 bits (557), Expect = 6.5e-54
Identity = 114/201 (56.72%), Postives = 134/201 (66.67%), Query Frame = 1


Query: 1   MMMLHKSLAIFGYACLFMAITSIA-QF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNLT 60
           MMMLHKSLAIF   C FMAI S A QF  PPV DT+G+PL+R  EY+I P  TDV GNLT
Sbjct: 1   MMMLHKSLAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 60

Query: 61  LKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQ 120
           L +R    CP +VGQ P+ S   G  V+  P   G+D I EG  L  VFQ  + CIT TQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 120

Query: 121 WRVDAAESDTGRRFVGIGREDGPAGIFGISR-DNGVYNIVWCPA---MMGRPRCGRAGIL 180
           W+V  A+  TGRRFV +G ++   G F I + D GVYNI WCP+   + GRPRCG AGIL
Sbjct: 121 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 180

Query: 181 VENGVRLLALDGEAFPFEFVK 195
           +E GVR LALDG AFPFEFV+
Sbjct: 181 IEKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of ClCG02G016000 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 209.5 bits (532), Expect = 5.2e-51
Identity = 111/202 (54.95%), Postives = 132/202 (65.35%), Query Frame = 1


Query: 1   MMMLHKSL-AIFGYACLFMAITSIA-QF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNL 60
           MM  HKSL +IF   C FMAI S A QF  PPV DT+G+PL+R  EY+I P  TDV GNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITST 120
           TL +R    CP +VGQ P+ S   G  V+  P   G+D I EG  L  VFQ  + CIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWRVDAAESDTGRRFVGIGREDGPAGIFGISR-DNGVYNIVWCPA---MMGRPRCGRAGI 180
           QW+V  A+  TGRRFV +G ++   G F I + D GVYNI WCP+   + GRPRCG AGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LVENGVRLLALDGEAFPFEFVK 195
           L+E GVR LALDG AFPFEFV+
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVR 202

The following BLAST results are available for this feature:

BLAST of ClCG02G016000 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MIRA_SYNDU	6.8e-19	35.07	Miraculin OS=Synsepalum dulcificum PE=1 SV=3	[more]
IAAS_HORVU	1.1e-13	39.17	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2	[more]
IAAS_WHEAT	1.5e-13	40.83	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1	[more]
IAAS_ORYSJ	3.6e-12	34.55	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ...	[more]
ASP_THECC	7.5e-10	32.45	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]

BLAST of ClCG02G016000 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIK2_CUCSA	1.8e-87	89.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1	[more]
A0A0A0LGB4_CUCSA	5.4e-55	56.72	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1	[more]
B9SIQ2_RICCO	3.3e-44	49.74	Alpha-amylase/subtilisin inhibitor, putative OS=Ricinus communis GN=RCOM_0538680...	[more]
A0A0X7YK90_HEVBR	1.0e-42	49.49	Alpha-amylase/subtilisin inhibitor OS=Hevea brasiliensis GN=ASI PE=2 SV=1	[more]
B9N9S5_POPTR	6.8e-42	48.77	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0019s02570g PE=4 SV=1	[more]

BLAST of ClCG02G016000 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17860.1	1.9e-11	29.65	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73325.1	1.9e-11	30.46	Kunitz family trypsin and protease inhibitor protein	[more]

BLAST of ClCG02G016000 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442847\|ref\|XP_004139192.1\|	9.6e-98	90.26	PREDICTED: miraculin-like [Cucumis sativus]	[more]
gi\|700205760\|gb\|KGN60879.1\|	2.6e-87	89.33	hypothetical protein Csa_2G021500 [Cucumis sativus]	[more]
gi\|449442849\|ref\|XP_004139193.1\|	7.7e-55	56.72	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus]	[more]
gi\|659070428\|ref\|XP_008455022.1\|	6.5e-54	56.72	PREDICTED: miraculin-like isoform X2 [Cucumis melo]	[more]
gi\|659070426\|ref\|XP_008455014.1\|	5.2e-51	54.95	PREDICTED: miraculin-like isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG02G016000.1	ClCG02G016000.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..194 score: 1.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..195 score: 1.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 27..195 score: 4.3
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 27..195 score: 9.8
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..195 score: 1.1
None	No IPR available	PANTHER	PTHR33107:SF4	SUBFAMILY NOT NAMED	coord: 1..195 score: 1.1

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG02G016000	Wax gourd	wcgwgoB269
ClCG02G016000	Cucumber (Gy14) v1	cgywcgB103
ClCG02G016000	Cucurbita maxima (Rimu)	cmawcgB156
ClCG02G016000	Cucurbita maxima (Rimu)	cmawcgB695
ClCG02G016000	Cucurbita moschata (Rifu)	cmowcgB144
ClCG02G016000	Cucurbita moschata (Rifu)	cmowcgB700
ClCG02G016000	Wild cucumber (PI 183967)	cpiwcgB128
ClCG02G016000	Cucumber (Chinese Long) v2	cuwcgB122
ClCG02G016000	Melon (DHL92) v3.5.1	mewcgB375
ClCG02G016000	Watermelon (97103) v1	wcgwmB197
ClCG02G016000	Cucurbita pepo (Zucchini)	cpewcgB104
ClCG02G016000	Cucurbita pepo (Zucchini)	cpewcgB733
ClCG02G016000	Bottle gourd (USVL1VR-Ls)	lsiwcgB098
ClCG02G016000	Cucumber (Gy14) v2	cgybwcgB119
ClCG02G016000	Melon (DHL92) v3.6.1	medwcgB369
ClCG02G016000	Silver-seed gourd	carwcgB0692
ClCG02G016000	Silver-seed gourd	carwcgB0883
ClCG02G016000	Cucumber (Chinese Long) v3	cucwcgB137
ClCG02G016000	Watermelon (97103) v2	wcgwmbB138