ClCG01G008380 (gene) Watermelon (Charleston Gray)

NameClCG01G008380
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
Description2-succinylbenzoate-CoA ligase
LocationCG_Chr01 : 9805970 .. 9807643 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAATTTGAAGCCACGCCCTGCTAATTCCTCTCCCCTCACTCCAATCGGCTTCATCGAAAGAACCGCCCTCATTTATGGCGATTCTCCCTCGATCATCTACAACTCCACCACTTACACCTGGTCGGAAACCCATCGCCGATGCCTTCAACTCGCCTCTTCCCTCTCTTCCATTGGCATCCAAAGAGGCCACGTCGTCTCCGTTATCGCTCCCAACACTCCGGCCATGTACGAACTCCACTTCGCCGTTCCGATGGTTGGTGCCGTCCTTAATACCATCAATCTCCGCCTCGACCCTCGAACCATCTCCGTCCTCCTCCGCCACAGCGAAACCAAACTCATCTTCGTCGATCAAGCCTCTTGTGCTCTAATCTCCGACGCTTTGGCCTTGTTCCCTTCGGAGACGAAACGGCCGTTACTCGTCCTCATTGCCGACGACACTGCGGTACAAGATTCGTCATTGTCATCCTCTGTTTTGATTCGCAACGATTTTGTCGATTCGTACGAGGAAATGGTTTGGAAAGGGGATTCTAATTTCGAATGGATAAGACCGGTGAGTGAATGGGATCCGATGGTTTTGAATTACACTTCTGGAACAACGTCGTCGCCGAAAGGAGTGGTTCATTGTCACAGGGGAATTTTCACGGTGGCACTCGATTCGCTTGTGGAATGGGGAGTTCCTAAGCAATCAGTTTATTTATGGACTTTACCAATGTTTCATGCCAATGGGTGGAGTTTTCCTTGGGGAATTGCTGCCGTAGGTGGAACAAATGTTTGTATTCGAAAATTCGATGCATCATTAATTTTTTCCCTGATTCGTCGCCATCATGTGACACACTTATGTGGAGCTCCGGTCGTACTCAATATGCTAACGAACTCTCCTGACAATCGACCACTTGATCGACCGGTCAAGATTCTGACTGCCGGAGCACCACCGCCTGCGGCAGTTCTGCTTCGAATTGAATCATTGGGGTTTGTTGTCACTCATGGGTATGGATTAACTGAGAGTGGTGGGACGATCATCAGCTGTTCATGGAAGTCGGAGTGGAACCGGCTGACGCAAACAGAGCAAGCACGGCTGAAGGCGAGGCAGGGGGTACGGATGCTAGCGGTTGAGGCGGCGGATGTGTTAGATCCAAAGACTAGAAAAAGTGTGAAAAGAGACGGAGCTTCCATAGGAGAGATTGTTGTGAGAGGCGGATCGATAATGCTTGGGTATTTGAAAGATCCAGAAGCGACAGCGAAAGCGATGACCGACGACGGGTGGTTCTTCACCGGAGATGTGGGGGTGATGCATCCAGATGGGTATATAGAGATAAAGGACAGATCGAAGGATGTGATTATTAGTGGAGGAGAGAATTTGAGCAGCGTTGAGGTGGAATCAGTTCTGTACATGAATCCGGTGGTGAATGAAGTGGCAGTAGTGGCACGACCGGACGAGTTCTGGGGAGAGACACCGTGTGCTTTTGTGAACTTGAAAAAAGGATTGACTCGACAACCGACGGAGGAAGAGATTATTGAGTTTTGTAAAGGGAAGCTGCCACGATTTATGGTGCCTAAGACGGTGGTCTTCACCACGGAGCTTCCAAAGACATCAACCGGAAAAATTCTGAAGTCTGTGCTCAGAGAAATGGCCAAGTCAATGGGCCCCACACCAAAAAGTAGATTGTAA

mRNA sequence

ATGGAAAATTTGAAGCCACGCCCTGCTAATTCCTCTCCCCTCACTCCAATCGGCTTCATCGAAAGAACCGCCCTCATTTATGGCGATTCTCCCTCGATCATCTACAACTCCACCACTTACACCTGGTCGGAAACCCATCGCCGATGCCTTCAACTCGCCTCTTCCCTCTCTTCCATTGGCATCCAAAGAGGCCACGTCGTCTCCGTTATCGCTCCCAACACTCCGGCCATGTACGAACTCCACTTCGCCGTTCCGATGGTTGGTGCCGTCCTTAATACCATCAATCTCCGCCTCGACCCTCGAACCATCTCCGTCCTCCTCCGCCACAGCGAAACCAAACTCATCTTCGTCGATCAAGCCTCTTGTGCTCTAATCTCCGACGCTTTGGCCTTGTTCCCTTCGGAGACGAAACGGCCGTTACTCGTCCTCATTGCCGACGACACTGCGGTACAAGATTCGTCATTGTCATCCTCTGTTTTGATTCGCAACGATTTTGTCGATTCGTACGAGGAAATGGTTTGGAAAGGGGATTCTAATTTCGAATGGATAAGACCGGTGAGTGAATGGGATCCGATGGTTTTGAATTACACTTCTGGAACAACGTCGTCGCCGAAAGGAGTGGTTCATTGTCACAGGGGAATTTTCACGGTGGCACTCGATTCGCTTGTGGAATGGGGAGTTCCTAAGCAATCAGTTTATTTATGGACTTTACCAATGTTTCATGCCAATGGGTGGAGTTTTCCTTGGGGAATTGCTGCCGTAGGTGGAACAAATGTTTGTATTCGAAAATTCGATGCATCATTAATTTTTTCCCTGATTCGTCGCCATCATGTGACACACTTATGTGGAGCTCCGGTCGTACTCAATATGCTAACGAACTCTCCTGACAATCGACCACTTGATCGACCGGTCAAGATTCTGACTGCCGGAGCACCACCGCCTGCGGCAGTTCTGCTTCGAATTGAATCATTGGGGTTTGTTGTCACTCATGGGTATGGATTAACTGAGAGTGGTGGGACGATCATCAGCTGTTCATGGAAGTCGGAGTGGAACCGGCTGACGCAAACAGAGCAAGCACGGCTGAAGGCGAGGCAGGGGGTACGGATGCTAGCGGTTGAGGCGGCGGATGTGTTAGATCCAAAGACTAGAAAAAGTGTGAAAAGAGACGGAGCTTCCATAGGAGAGATTGTTGTGAGAGGCGGATCGATAATGCTTGGGTATTTGAAAGATCCAGAAGCGACAGCGAAAGCGATGACCGACGACGGGTGGTTCTTCACCGGAGATGTGGGGGTGATGCATCCAGATGGGTATATAGAGATAAAGGACAGATCGAAGGATGTGATTATTAGTGGAGGAGAGAATTTGAGCAGCGTTGAGGTGGAATCAGTTCTGTACATGAATCCGGTGGTGAATGAAGTGGCAGTAGTGGCACGACCGGACGAGTTCTGGGGAGAGACACCGTGTGCTTTTGTGAACTTGAAAAAAGGATTGACTCGACAACCGACGGAGGAAGAGATTATTGAGTTTTGTAAAGGGAAGCTGCCACGATTTATGGTGCCTAAGACGGTGGTCTTCACCACGGAGCTTCCAAAGACATCAACCGGAAAAATTCTGAAGTCTGTGCTCAGAGAAATGGCCAAGTCAATGGGCCCCACACCAAAAAGTAGATTGTAA

Coding sequence (CDS)

ATGGAAAATTTGAAGCCACGCCCTGCTAATTCCTCTCCCCTCACTCCAATCGGCTTCATCGAAAGAACCGCCCTCATTTATGGCGATTCTCCCTCGATCATCTACAACTCCACCACTTACACCTGGTCGGAAACCCATCGCCGATGCCTTCAACTCGCCTCTTCCCTCTCTTCCATTGGCATCCAAAGAGGCCACGTCGTCTCCGTTATCGCTCCCAACACTCCGGCCATGTACGAACTCCACTTCGCCGTTCCGATGGTTGGTGCCGTCCTTAATACCATCAATCTCCGCCTCGACCCTCGAACCATCTCCGTCCTCCTCCGCCACAGCGAAACCAAACTCATCTTCGTCGATCAAGCCTCTTGTGCTCTAATCTCCGACGCTTTGGCCTTGTTCCCTTCGGAGACGAAACGGCCGTTACTCGTCCTCATTGCCGACGACACTGCGGTACAAGATTCGTCATTGTCATCCTCTGTTTTGATTCGCAACGATTTTGTCGATTCGTACGAGGAAATGGTTTGGAAAGGGGATTCTAATTTCGAATGGATAAGACCGGTGAGTGAATGGGATCCGATGGTTTTGAATTACACTTCTGGAACAACGTCGTCGCCGAAAGGAGTGGTTCATTGTCACAGGGGAATTTTCACGGTGGCACTCGATTCGCTTGTGGAATGGGGAGTTCCTAAGCAATCAGTTTATTTATGGACTTTACCAATGTTTCATGCCAATGGGTGGAGTTTTCCTTGGGGAATTGCTGCCGTAGGTGGAACAAATGTTTGTATTCGAAAATTCGATGCATCATTAATTTTTTCCCTGATTCGTCGCCATCATGTGACACACTTATGTGGAGCTCCGGTCGTACTCAATATGCTAACGAACTCTCCTGACAATCGACCACTTGATCGACCGGTCAAGATTCTGACTGCCGGAGCACCACCGCCTGCGGCAGTTCTGCTTCGAATTGAATCATTGGGGTTTGTTGTCACTCATGGGTATGGATTAACTGAGAGTGGTGGGACGATCATCAGCTGTTCATGGAAGTCGGAGTGGAACCGGCTGACGCAAACAGAGCAAGCACGGCTGAAGGCGAGGCAGGGGGTACGGATGCTAGCGGTTGAGGCGGCGGATGTGTTAGATCCAAAGACTAGAAAAAGTGTGAAAAGAGACGGAGCTTCCATAGGAGAGATTGTTGTGAGAGGCGGATCGATAATGCTTGGGTATTTGAAAGATCCAGAAGCGACAGCGAAAGCGATGACCGACGACGGGTGGTTCTTCACCGGAGATGTGGGGGTGATGCATCCAGATGGGTATATAGAGATAAAGGACAGATCGAAGGATGTGATTATTAGTGGAGGAGAGAATTTGAGCAGCGTTGAGGTGGAATCAGTTCTGTACATGAATCCGGTGGTGAATGAAGTGGCAGTAGTGGCACGACCGGACGAGTTCTGGGGAGAGACACCGTGTGCTTTTGTGAACTTGAAAAAAGGATTGACTCGACAACCGACGGAGGAAGAGATTATTGAGTTTTGTAAAGGGAAGCTGCCACGATTTATGGTGCCTAAGACGGTGGTCTTCACCACGGAGCTTCCAAAGACATCAACCGGAAAAATTCTGAAGTCTGTGCTCAGAGAAATGGCCAAGTCAATGGGCCCCACACCAAAAAGTAGATTGTAA

Protein sequence

MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKSVLREMAKSMGPTPKSRL
BLAST of ClCG01G008380 vs. Swiss-Prot
Match: AAE5_ARATH (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 PE=2 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.4e-218
Identity = 367/558 (65.77%), Postives = 455/558 (81.54%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTT-YTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH  +KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++  ++   P+LV IAD    ++    + V  R  F  +Y++++ +GD +
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+RPV ILTAGAPPPAAVLLR ES+GFV++HGYGLTE+ G  +SC+WK +WNRL  +++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++ +SV+R+G ++GEIV+RG SIMLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           D FWGETPCAFV+LK GLT++PTE E+IE+C+ K+P++MVPKTV F  ELPKTSTGK++K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 SVLREMAKSMGPTPKSRL 558
            VLRE+AK MG T  SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of ClCG01G008380 vs. Swiss-Prot
Match: AAE6_ARATH (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 2.0e-212
Identity = 354/551 (64.25%), Postives = 447/551 (81.13%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTT-YTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++  ++   P+LV+IAD    ++    + V   + F  +Y++++ +GD  
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIADK---EEEGGVADVADLSKFSYTYDDLIERGDPG 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+ PV ILTAG+PPPA VLLR ES+GFV++HGYGLTE+ G I+SC+WK +WN L  +++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++  SV+R+G ++GEIV+RG S+MLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           DEFWGETPCAFV+LK G + +PTEEE++E+C+ K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 SVLREMAKSMG 551
            VLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of ClCG01G008380 vs. Swiss-Prot
Match: AAE8_ARATH (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana GN=AAE8 PE=2 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 6.8e-205
Identity = 350/551 (63.52%), Postives = 436/551 (79.13%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSLSSI 60
           ME+LKP  ANS PLT +GF+ER A +YGD  SI+Y NST YTW ET+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
               L  +A+ +  +    P+LVLIA++   ++    + V  R+ F   Y +++ +G+ +
Sbjct: 121 FYSDLAVEAITMLLNP---PILVLIANE---EEEEGGAEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+V+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN-R 300
           FHANGW++PWGIAAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S ++ +
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PV  LTAG+ PPA VLLR ESLGF+V+HGYGLTE+ G I+SC+WK  WNRL  ++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           A+LK+RQGVR +     DV+DP++ +SV+RDG ++GEIV+RG SIMLGYLK+P  T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
            + GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNE AVVAR
Sbjct: 421 KN-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PDEFWGETPCAFV+LK GLTR+PT++EIIE+CK K+PR+M PKTV F  ELPKTSTGKI+
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KSVLREMAKSM 550
           KS+L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of ClCG01G008380 vs. Swiss-Prot
Match: AEE9_ARATH (Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 7.0e-202
Identity = 340/557 (61.04%), Postives = 432/557 (77.56%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTT-YTWSETHRRCLQLASSL--S 60
           ME L P P+NS+PLT +GF++R A +YGD PSI++ + T +TWSETH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFV 120
           S+GI RG VVSV+ PN P++YEL FAVPM GA+LN IN RLD   +SVLLRHSE+KL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGD 180
           D  S +++ +A++ F  + ++P LVL+ DD   +D SLS S    +DF+D+Y+ ++ +GD
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSAA--SDFLDTYQGVMERGD 180

Query: 181 SNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTL 240
           S F+WIRP +EW PM+LNYTSGTTSSPKGVV  HR IF + + SL++W  P + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTL 240

Query: 241 PMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFHANGW + WG AAVG TNVC R+ DA  I+ LI +HHVTH+C AP+VLNMLTN P  
Sbjct: 241 PMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSR 300

Query: 301 RPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTE 360
           +PL  PV+++TAGAPPPAA++ R E+LGF V HGYGLTE+GG ++SC+WK+EW+ L   E
Sbjct: 301 KPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKA 420
           +ARLK+RQGVR +     DV DP+T KSV+ DG S+GEIV++GGS+MLGY KDPE TA  
Sbjct: 361 RARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAAC 420

Query: 421 MTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVA 480
           M +DGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NPVV E AVVA
Sbjct: 421 MREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFVNLK-----KGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKT 540
           +PD+ WGETPCAFV+LK      GL    TE EI EFCK +LP++MVP+ V+F  ELPKT
Sbjct: 481 KPDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKT 540

Query: 541 STGKILKSVLREMAKSM 550
           STGKI K +LR+MAKS+
Sbjct: 541 STGKIQKFLLRQMAKSL 549

BLAST of ClCG01G008380 vs. Swiss-Prot
Match: AAE4_ARATH (Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana GN=AEE4 PE=2 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.7e-198
Identity = 329/551 (59.71%), Postives = 420/551 (76.23%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSS-- 60
           ME L P  +NS PLT +GF+ER A ++GDSPS+++ +T +TWSETH RCL++AS+LSS  
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVD 120
           +GI RG VVSVI PN P++YEL FAVPM GAVLN IN RLD   +SVLLRHSE+KL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDS 180
             S +L+ +A++  P + +RP LV++ D   +  SS +       DF+D+YE  + +GD 
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILNDGNDMPSSSSADM-----DFLDTYEGFMERGDL 180

Query: 181 NFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLP 240
            F+W+RP SEW PMVLNYTSGTTSSPKGVVH HR +F   ++SL++W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           MFHANGWS+ W  AAVG  N+C+ + D   IF+LI ++ VTH+C AP+VLNMLTN P  +
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PVK++TAGAPPPA V+ + E+LGF V+HGYG+TE+GG ++SC+ K EW+RL   E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           A+ K+RQG+R       DV DP + KSVK DGA++GEIV RGGS+MLGY KDPE TA +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
            +DGWF+TGD+GVMHPDGY+E+KDRSKDV+I GGEN+SS E+E+VLY NP + E AVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PD+ WGETPCAFV+LK       TE EI EFCK KLP++MVP+ VVF  ELPKTSTGKI 
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540

Query: 541 KSVLREMAKSM 550
           K +LR+MAKS+
Sbjct: 541 KFLLRQMAKSL 544

BLAST of ClCG01G008380 vs. TrEMBL
Match: A0A0A0KMT3_CUCSA (AMP dependent ligase OS=Cucumis sativus GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 6.6e-284
Identity = 479/557 (86.00%), Postives = 517/557 (92.82%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKPRPANS PLTPIGFIER A+IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GA+LNTINLRLD RTIS+LLRHSE+KLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI DALALFP + KRPLLVLI DDTA QDSSL S V IR++FVDSYE MV  GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTS+PKGVV+CHRG+FTVALDS++EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA+VLLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+MLGYLKD EAT+KAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLP+FMVPKTV+F TE+PKT+TGK  K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of ClCG01G008380 vs. TrEMBL
Match: A0A0A0KT33_CUCSA (AMP dependent ligase OS=Cucumis sativus GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 1.5e-264
Identity = 453/557 (81.33%), Postives = 498/557 (89.41%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANSSPLTPIGF+ER +LIY DSPSIIY+STT+TWS+T+RRCL+LASSLSSIG
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           I +GHVVSVIAPNTP MYEL FAVPM GA+L+ INLRLD RTISV LRHSE+KL+FVDQA
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           S  LISDALALFP E KRPLLVLI+DD AV+DS+  SSV I  D VD YE+MV KGD  F
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSA--SSVAIGGDVVDLYEDMVQKGDPEF 201

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
            WIRPVSEWDP+V+NYTSGTTSSPKGVVH HRGIFTV LDSL+EWGVPK SVYLWTLPMF
Sbjct: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFP+ IAAVGGTN+CIRKFDA+LIFSLI RHHVTH+CGAPVVLNMLTNSP+NRPL
Sbjct: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           +RPVKILTAGAPPPAAVLLR ESLGF V+HGYGLTE+GG +I CSWK EWN+L  TE+AR
Sbjct: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVR LA+  ADVLD  T K V+RDG SIGEIV+RGGS+MLGY KDPEAT KAMT+
Sbjct: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVVARPD
Sbjct: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAFV+L++GLTR PTEEEIIE+CKGKL  FMVPKTVVF  +LPKTSTGKI K 
Sbjct: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561

Query: 541 VLREMAKSMGPTPKSRL 558
           +LREMAKSMG +PKSRL
Sbjct: 562 LLREMAKSMGCSPKSRL 576

BLAST of ClCG01G008380 vs. TrEMBL
Match: B9S6X4_RICCO (AMP dependent ligase, putative OS=Ricinus communis GN=RCOM_1328840 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.6e-237
Identity = 394/558 (70.61%), Postives = 469/558 (84.05%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           M++LKP+PANS PLTP+ F+ER + +YGD PSI+YN+ TYTWS+THRRCLQLASSLSS G
Sbjct: 1   MDHLKPKPANSFPLTPLSFLERASTVYGDCPSIVYNTATYTWSQTHRRCLQLASSLSSGG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
             RG VVSV+APNTP MYEL FAVPM GAVLN IN RLD RT+SVLLRHS++KL+FVD  
Sbjct: 61  FTRGDVVSVVAPNTPPMYELQFAVPMSGAVLNNINTRLDARTVSVLLRHSQSKLVFVDCL 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDT-AVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
           S  L+ +A++LFPS  KRP LVLI DD  A   SS SSS  +  DF  +YE MV KGD  
Sbjct: 121 SRDLVLEAVSLFPSSVKRPTLVLITDDDEAPASSSTSSSSTV--DFCCTYESMVEKGDPE 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+W++P SEWDPM+LNYTSGTTSSPKGVVHCHRGI+ + ++SL++W VP+Q VYLWTLP+
Sbjct: 181 FKWVQPQSEWDPMILNYTSGTTSSPKGVVHCHRGIYIITVESLIDWNVPRQPVYLWTLPL 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FHANGWSFPWG+AAVGGTN+CIRKFDAS I+ LI+RH VTH+CGAPVVLNMLTNSPDN P
Sbjct: 241 FHANGWSFPWGMAAVGGTNICIRKFDASSIYGLIKRHGVTHMCGAPVVLNMLTNSPDNEP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L RPV ILTAGAPPPAAVL R ESLGFVV+HGYGLTE+ G ++SC+WK +WN +  +E+A
Sbjct: 301 LKRPVHILTAGAPPPAAVLFRTESLGFVVSHGYGLTETAGLVVSCAWKPKWNTVPASERA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR+      DV+DPKT  SVKRDG S+GEIV+RGG +M+GYLKDP+ T+K + 
Sbjct: 361 RLKARQGVRIAGFTEIDVIDPKTGLSVKRDGLSLGEIVLRGGCVMMGYLKDPDGTSKCLK 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
           DDGWF+TGDVGVMHPDGY+EIKDRSKDVIISGGEN+SSVEVESVLY NPVVNE AVVARP
Sbjct: 421 DDGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENISSVEVESVLYSNPVVNEAAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           DE+WGETPCAFV+L+ G   + +E++IIE+C+ K+P +MVPKTVVF  ELPKTSTGKI K
Sbjct: 481 DEYWGETPCAFVSLRDGAREKVSEKDIIEYCREKMPHYMVPKTVVFKEELPKTSTGKIQK 540

Query: 541 SVLREMAKSMGPTPKSRL 558
            VLR++AK+MG +  SR+
Sbjct: 541 FVLRDIAKAMGASKVSRM 556

BLAST of ClCG01G008380 vs. TrEMBL
Match: A0A061ESR0_THECC (Acyl activating enzyme 5 OS=Theobroma cacao GN=TCM_020359 PE=4 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 1.4e-236
Identity = 392/550 (71.27%), Postives = 470/550 (85.45%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME LKPRPA+SSPLTP+GF+ER A +YGD  SIIYN+T+YTWS+THRRCLQ+ASSLSS G
Sbjct: 51  MEELKPRPASSSPLTPLGFLERAATVYGDCTSIIYNNTSYTWSDTHRRCLQIASSLSSTG 110

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           I+RGHVVSV+APN PAMYELHFAVPM GAVLN+IN RLD R +S+LLRH E+KL+FVD  
Sbjct: 111 IKRGHVVSVLAPNIPAMYELHFAVPMTGAVLNSINTRLDARIVSILLRHCESKLLFVDTL 170

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           S +L  +A++LFP  TK PLLVLI DD+A  +SS SSS  + + F  +YE +V KGD +F
Sbjct: 171 SRSLALEAISLFPPNTKPPLLVLIGDDSADAESSDSSSSTVDSRFCCTYESLVKKGDPDF 230

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +W+RP SEW+P+VLNYTSGTTSSPKGVVH HRG F + LDSL++WGVPKQ VYLWTLPMF
Sbjct: 231 KWVRPQSEWNPIVLNYTSGTTSSPKGVVHSHRGTFMMTLDSLIDWGVPKQPVYLWTLPMF 290

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSF WGIAAVGG NVC+RKFDA +I+SLI++H VTH+CGAPVVL+ML+N P+ +PL
Sbjct: 291 HANGWSFTWGIAAVGGINVCVRKFDAPIIYSLIKKHGVTHMCGAPVVLSMLSNFPEIKPL 350

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
             PVKILTAGAPPPAAVL R E+LGF+V+HGYGLTE+GG ++SC+WK  WN+L  TE+AR
Sbjct: 351 QSPVKILTAGAPPPAAVLFRTEALGFIVSHGYGLTETGGLVVSCAWKRGWNKLPATERAR 410

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVR L +  ADV+D ++  SVKRDG+++GEIV+RG  IMLGYLKDP  T K M +
Sbjct: 411 LKARQGVRTLGMTEADVVDTESGLSVKRDGSTLGEIVLRGACIMLGYLKDPNGTNKCMKE 470

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P + E AVVARPD
Sbjct: 471 NGWFFTGDVGVMHPDGYMEIKDRSKDVIISGGENLSSVEVESILYNHPAIIEAAVVARPD 530

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           ++WGETPCAFV+LK GLT++P+E+EIIE+C+ KLP +MVPKTVVF  ELPKTSTGKI K 
Sbjct: 531 DYWGETPCAFVSLKAGLTQKPSEKEIIEYCRAKLPHYMVPKTVVFKEELPKTSTGKIQKF 590

Query: 541 VLREMAKSMG 551
           +LRE+AK MG
Sbjct: 591 LLREIAKGMG 600

BLAST of ClCG01G008380 vs. TrEMBL
Match: A0A068U0B9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00036869001 PE=4 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 4.4e-235
Identity = 386/559 (69.05%), Postives = 475/559 (84.97%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           M+ LKP PANS+PLTP+GF+ER A +YGD PS++YN+ TYTWS+TH RCL++ASS+ S+G
Sbjct: 1   MDELKPGPANSAPLTPLGFLERAATVYGDCPSVVYNNATYTWSDTHVRCLRVASSIVSLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           I+RG VVSV+APN PAMYE HFAVPM GAVL+TINLRLD RT+S+LL HSE+KLI VD  
Sbjct: 61  IKRGQVVSVVAPNIPAMYEAHFAVPMAGAVLSTINLRLDARTVSILLHHSESKLILVDYQ 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRN--DFVDSYEEMVWKGDS 180
           S +L+ +AL+LFP  ++RPLLVLI DD        S S+   N  DF  +YE +V KGD 
Sbjct: 121 STSLVLEALSLFPPNSQRPLLVLITDDA-------SPSIPPSNFPDFYCTYESLVQKGDP 180

Query: 181 NFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLP 240
            F+W+RP S++DP++LNYTSGTTSSPKGVVHCHRGIF V +DSL+EW VPK+ VYLWTLP
Sbjct: 181 GFKWVRPKSDFDPIILNYTSGTTSSPKGVVHCHRGIFIVTVDSLIEWSVPKEPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           MFHANGWS+PWG+AAVGGTN+C+RKFDA++++S IR+H+VTHLCGAPVVLNM++N P   
Sbjct: 241 MFHANGWSYPWGMAAVGGTNICLRKFDAAVVYSAIRQHNVTHLCGAPVVLNMISNFPMGE 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PV+I+TAGAPPPAAVLLR E+LGF V+HGYGLTE+GG ++SC+WK++WNRL  TE+
Sbjct: 301 PLKNPVEIMTAGAPPPAAVLLRTEALGFKVSHGYGLTETGGLVVSCTWKNKWNRLPATER 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           ARLKARQGVR L +   DV++P+T  SVKRDG+++GE+V+RGG IMLGYLKDPE T+K+ 
Sbjct: 361 ARLKARQGVRTLGMAEIDVIEPETGVSVKRDGSTLGELVLRGGCIMLGYLKDPEGTSKST 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
            ++GWF+TGDV VMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYM+P VNE AVVAR
Sbjct: 421 RENGWFYTGDVAVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMHPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PDEFWGETPCAFV+LK  + ++P E+++IEFC+GKLP++MVPKTVVF  ELPKTSTGKI 
Sbjct: 481 PDEFWGETPCAFVSLKADVKQRPAEKDVIEFCRGKLPKYMVPKTVVFRQELPKTSTGKIQ 540

Query: 541 KSVLREMAKSMGPTPKSRL 558
           K VLREMAK++G +  SRL
Sbjct: 541 KFVLREMAKAIGSSKVSRL 552

BLAST of ClCG01G008380 vs. TAIR10
Match: AT5G16370.1 (AT5G16370.1 acyl activating enzyme 5)

HSP 1 Score: 760.4 bits (1962), Expect = 7.9e-220
Identity = 367/558 (65.77%), Postives = 455/558 (81.54%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTT-YTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH  +KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++  ++   P+LV IAD    ++    + V  R  F  +Y++++ +GD +
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIADK---EEEGGDADVADRTKFSYTYDDLIHRGDLD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FHANGWS+PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + + +P
Sbjct: 241 FHANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+RPV ILTAGAPPPAAVLLR ES+GFV++HGYGLTE+ G  +SC+WK +WNRL  +++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++ +SV+R+G ++GEIV+RG SIMLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY NP VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           D FWGETPCAFV+LK GLT++PTE E+IE+C+ K+P++MVPKTV F  ELPKTSTGK++K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540

Query: 541 SVLREMAKSMGPTPKSRL 558
            VLRE+AK MG T  SR+
Sbjct: 541 FVLREIAKKMGTTRLSRM 552

BLAST of ClCG01G008380 vs. TAIR10
Match: AT5G16340.1 (AT5G16340.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 740.0 bits (1909), Expect = 1.1e-213
Identity = 354/551 (64.25%), Postives = 447/551 (81.13%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTT-YTWSETHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGF+ER A +YGD  SI+Y S T YTW ET+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++PNTPAMYEL FAVPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
            S  L  +A+++  ++   P+LV+IAD    ++    + V   + F  +Y++++ +GD  
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIADK---EEEGGVADVADLSKFSYTYDDLIERGDPG 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLP+
Sbjct: 181 FKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRP 300
           FH+NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTH+CGAPVVLNML+ + +++P
Sbjct: 241 FHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQP 300

Query: 301 LDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQA 360
           L+ PV ILTAG+PPPA VLLR ES+GFV++HGYGLTE+ G I+SC+WK +WN L  +++A
Sbjct: 301 LNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRA 360

Query: 361 RLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMT 420
           RLKARQGVR +     DV+DP++  SV+R+G ++GEIV+RG S+MLGYLKDP  T KA+ 
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL- 420

Query: 421 DDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARP 480
            +GWF+TGDVGV+H DGY+EIKDRSKD+II+GGEN+SSVEVE+VLY  P VNEVAVVARP
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 DEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           DEFWGETPCAFV+LK G + +PTEEE++E+C+ K+P++MVPKTV F  ELPK+STGK+ K
Sbjct: 481 DEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTK 540

Query: 541 SVLREMAKSMG 551
            VLR++AK MG
Sbjct: 541 FVLRDIAKKMG 545

BLAST of ClCG01G008380 vs. TAIR10
Match: AT1G75960.1 (AT1G75960.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 714.9 bits (1844), Expect = 3.8e-206
Identity = 350/551 (63.52%), Postives = 436/551 (79.13%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIY-NSTTYTWSETHRRCLQLASSLSSI 60
           ME+LKP  ANS PLT +GF+ER A +YGD  SI+Y NST YTW ET+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQ 120
           GI R  VVSV++ NTP MYEL F+VPM GA+LN IN RLD RT+SVLLRH E+KL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSN 180
               L  +A+ +  +    P+LVLIA++   ++    + V  R+ F   Y +++ +G+ +
Sbjct: 121 FYSDLAVEAITMLLNP---PILVLIANE---EEEEGGAEVTERSKFCYLYSDLITRGNPD 180

Query: 181 FEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPM 240
           F+WIRP SEWDP+V+NYTSGTTSSPKGVVHCHRGIF + LDSL +W VPK  VYLWTLP+
Sbjct: 181 FKWIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPI 240

Query: 241 FHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN-R 300
           FHANGW++PWGIAAVGGTNVC+RK  A  I+ LIR H VTH+ GAP+VL +L+ S ++ +
Sbjct: 241 FHANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQ 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PV  LTAG+ PPA VLLR ESLGF+V+HGYGLTE+ G I+SC+WK  WNRL  ++Q
Sbjct: 301 PLKSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQ 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           A+LK+RQGVR +     DV+DP++ +SV+RDG ++GEIV+RG SIMLGYLK+P  T  + 
Sbjct: 361 AQLKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
            + GWFFTGD+GV+H DGY+EIKDRSKDVIISGGEN+SSVEVE+VLY NP VNE AVVAR
Sbjct: 421 KN-GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVAR 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PDEFWGETPCAFV+LK GLTR+PT++EIIE+CK K+PR+M PKTV F  ELPKTSTGKI+
Sbjct: 481 PDEFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKII 540

Query: 541 KSVLREMAKSM 550
           KS+L+E+AK+M
Sbjct: 541 KSLLKEIAKNM 544

BLAST of ClCG01G008380 vs. TAIR10
Match: AT1G21540.1 (AT1G21540.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 704.9 bits (1818), Expect = 3.9e-203
Identity = 340/557 (61.04%), Postives = 432/557 (77.56%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTT-YTWSETHRRCLQLASSL--S 60
           ME L P P+NS+PLT +GF++R A +YGD PSI++ + T +TWSETH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFV 120
           S+GI RG VVSV+ PN P++YEL FAVPM GA+LN IN RLD   +SVLLRHSE+KL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGD 180
           D  S +++ +A++ F  + ++P LVL+ DD   +D SLS S    +DF+D+Y+ ++ +GD
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSAA--SDFLDTYQGVMERGD 180

Query: 181 SNFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTL 240
           S F+WIRP +EW PM+LNYTSGTTSSPKGVV  HR IF + + SL++W  P + VYLWTL
Sbjct: 181 SRFKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTL 240

Query: 241 PMFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDN 300
           PMFHANGW + WG AAVG TNVC R+ DA  I+ LI +HHVTH+C AP+VLNMLTN P  
Sbjct: 241 PMFHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSR 300

Query: 301 RPLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTE 360
           +PL  PV+++TAGAPPPAA++ R E+LGF V HGYGLTE+GG ++SC+WK+EW+ L   E
Sbjct: 301 KPLKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLE 360

Query: 361 QARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKA 420
           +ARLK+RQGVR +     DV DP+T KSV+ DG S+GEIV++GGS+MLGY KDPE TA  
Sbjct: 361 RARLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAAC 420

Query: 421 MTDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVA 480
           M +DGWF++GDVGV+H DGY+E+KDRSKDVII GGEN+SS EVE+VLY NPVV E AVVA
Sbjct: 421 MREDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVA 480

Query: 481 RPDEFWGETPCAFVNLK-----KGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKT 540
           +PD+ WGETPCAFV+LK      GL    TE EI EFCK +LP++MVP+ V+F  ELPKT
Sbjct: 481 KPDKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKT 540

Query: 541 STGKILKSVLREMAKSM 550
           STGKI K +LR+MAKS+
Sbjct: 541 STGKIQKFLLRQMAKSL 549

BLAST of ClCG01G008380 vs. TAIR10
Match: AT1G77240.1 (AT1G77240.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 693.0 bits (1787), Expect = 1.5e-199
Identity = 329/551 (59.71%), Postives = 420/551 (76.23%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSS-- 60
           ME L P  +NS PLT +GF+ER A ++GDSPS+++ +T +TWSETH RCL++AS+LSS  
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVD 120
           +GI RG VVSVI PN P++YEL FAVPM GAVLN IN RLD   +SVLLRHSE+KL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 QASCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDS 180
             S +L+ +A++  P + +RP LV++ D   +  SS +       DF+D+YE  + +GD 
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILNDGNDMPSSSSADM-----DFLDTYEGFMERGDL 180

Query: 181 NFEWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLP 240
            F+W+RP SEW PMVLNYTSGTTSSPKGVVH HR +F   ++SL++W +P + VYLWTLP
Sbjct: 181 RFKWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLP 240

Query: 241 MFHANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNR 300
           MFHANGWS+ W  AAVG  N+C+ + D   IF+LI ++ VTH+C AP+VLNMLTN P  +
Sbjct: 241 MFHANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQK 300

Query: 301 PLDRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQ 360
           PL  PVK++TAGAPPPA V+ + E+LGF V+HGYG+TE+GG ++SC+ K EW+RL   E+
Sbjct: 301 PLQSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDER 360

Query: 361 ARLKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAM 420
           A+ K+RQG+R       DV DP + KSVK DGA++GEIV RGGS+MLGY KDPE TA +M
Sbjct: 361 AKQKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASM 420

Query: 421 TDDGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVAR 480
            +DGWF+TGD+GVMHPDGY+E+KDRSKDV+I GGEN+SS E+E+VLY NP + E AVVA+
Sbjct: 421 REDGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAK 480

Query: 481 PDEFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKIL 540
           PD+ WGETPCAFV+LK       TE EI EFCK KLP++MVP+ VVF  ELPKTSTGKI 
Sbjct: 481 PDKMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQ 540

Query: 541 KSVLREMAKSM 550
           K +LR+MAKS+
Sbjct: 541 KFLLRQMAKSL 544

BLAST of ClCG01G008380 vs. NCBI nr
Match: gi|659126420|ref|XP_008463174.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 998.0 bits (2579), Expect = 6.4e-288
Identity = 486/557 (87.25%), Postives = 521/557 (93.54%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANS PLTPIGFI+R A IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSVIAPNTPAMYELHFAVPMVGA+LNTINLRLD RTIS+LLRHSE+KLIFVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI +ALALFP E KRPLLVLIADD A QDSSL S V IR++FVDSYE+MV +GDS F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTSSPKGVVHCHRGIFTVALDSL+EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFDA LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA++LLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+ LGYLKDPE TAKAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLPRFMVPKTV+F TE+PKT+TGKI K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP PKSRL
Sbjct: 541 VLRQKAKSLGPAPKSRL 557

BLAST of ClCG01G008380 vs. NCBI nr
Match: gi|449462358|ref|XP_004148908.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 984.2 bits (2543), Expect = 9.5e-284
Identity = 479/557 (86.00%), Postives = 517/557 (92.82%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKPRPANS PLTPIGFIER A+IYGDS SIIYNSTTYTWSETHRRCLQLASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           IQRGHVVSV APNTPAMYELHFAVPM GA+LNTINLRLD RTIS+LLRHSE+KLIFVDQ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           SCALI DALALFP + KRPLLVLI DDTA QDSSL S V IR++FVDSYE MV  GDS F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           +WIRP+SEWDP+VLNYTSGTTS+PKGVV+CHRG+FTVALDS++EWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           H NGWSFPWGIAAVGGTNVCIRKFD+ LIFSLIRRHHVTHLCGAPVVLNMLTNSPDN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           DRPVKILTAGAPPPA+VLLR ESLGFVVTHGYGLTES GTII CSWKSEWN+L   EQAR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVRMLAVEA DVLDP+TRKSVKRDGASIGEIVVRG S+MLGYLKD EAT+KAM++
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNP+VNE AVVARPD
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAF++LK+GL+R+PTEEEIIE+CKGKLP+FMVPKTV+F TE+PKT+TGK  K 
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           VLR+ AKS+GP  KSRL
Sbjct: 541 VLRQKAKSLGPPQKSRL 557

BLAST of ClCG01G008380 vs. NCBI nr
Match: gi|449462360|ref|XP_004148909.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 919.8 bits (2376), Expect = 2.2e-264
Identity = 453/557 (81.33%), Postives = 498/557 (89.41%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP PANSSPLTPIGF+ER +LIY DSPSIIY+STT+TWS+T+RRCL+LASSLSSIG
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           I +GHVVSVIAPNTP MYEL FAVPM GA+L+ INLRLD RTISV LRHSE+KL+FVDQA
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           S  LISDALALFP E KRPLLVLI+DD AV+DS+  SSV I  D VD YE+MV KGD  F
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSA--SSVAIGGDVVDLYEDMVQKGDPEF 201

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
            WIRPVSEWDP+V+NYTSGTTSSPKGVVH HRGIFTV LDSL+EWGVPK SVYLWTLPMF
Sbjct: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFP+ IAAVGGTN+CIRKFDA+LIFSLI RHHVTH+CGAPVVLNMLTNSP+NRPL
Sbjct: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           +RPVKILTAGAPPPAAVLLR ESLGF V+HGYGLTE+GG +I CSWK EWN+L  TE+AR
Sbjct: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVR LA+  ADVLD  T K V+RDG SIGEIV+RGGS+MLGY KDPEAT KAMT+
Sbjct: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVVARPD
Sbjct: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAFV+L++GLTR PTEEEIIE+CKGKL  FMVPKTVVF  +LPKTSTGKI K 
Sbjct: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561

Query: 541 VLREMAKSMGPTPKSRL 558
           +LREMAKSMG +PKSRL
Sbjct: 562 LLREMAKSMGCSPKSRL 576

BLAST of ClCG01G008380 vs. NCBI nr
Match: gi|659126423|ref|XP_008463175.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 913.7 bits (2360), Expect = 1.6e-262
Identity = 446/557 (80.07%), Postives = 499/557 (89.59%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           M++LKP PANSSPLTPI F+ER +L+Y DSPSIIYNST++TWS+T+ RCL+LASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           I +GHVVSVI+PNTP MYEL F+VPM GA+L+ INLRLD RTISV LRHSE+KL+FVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           S ALISDAL LFP E+KRPLLVLIADD AV+DS+  SSV I  DFVD YE+M+ KGD  F
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSA--SSVAIGGDFVDFYEDMIQKGDPEF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
            WIRPVSEWDP+V+NYTSGTTSSPKGVVH HRGIFTV LDSL+EWGVPK SVYLWTLPMF
Sbjct: 181 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFP+ IAAVGGTN+CIRKFDA+LIFSLI RHHVTHLCGAPVVLNMLTNSP+NRPL
Sbjct: 241 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
           +RPVKILTAGAPPPAAVL R ESLGF V+HGYGLTE+GG +I CSWK +WN+L  TE+AR
Sbjct: 301 ERPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVR LA+  ADVLD +T KSVKRDG SIGEIV+RGGS+MLGY KDPEAT KAMT+
Sbjct: 361 LKARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTE 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           +GWFFTGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNE AVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLTRQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILKS 540
           EFWGETPCAFV+L++GL+R PTEEEIIE+C+GKL  FMVPKTVVF  +LPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 540

Query: 541 VLREMAKSMGPTPKSRL 558
           +LREMAKSMGP+ KSRL
Sbjct: 541 LLREMAKSMGPSRKSRL 555

BLAST of ClCG01G008380 vs. NCBI nr
Match: gi|1009151390|ref|XP_015893528.1| (PREDICTED: probable acyl-activating enzyme 6 [Ziziphus jujuba])

HSP 1 Score: 839.3 bits (2167), Expect = 3.8e-240
Identity = 403/560 (71.96%), Postives = 474/560 (84.64%), Query Frame = 1

Query: 1   MENLKPRPANSSPLTPIGFIERTALIYGDSPSIIYNSTTYTWSETHRRCLQLASSLSSIG 60
           ME+LKP P NSSPLTPIGF+ER A +YGD PSI+YN+TTYTWS+TH RCL++ASS+ S+G
Sbjct: 1   MEDLKPTPPNSSPLTPIGFLERAATVYGDCPSIVYNTTTYTWSQTHLRCLRVASSIQSLG 60

Query: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAVLNTINLRLDPRTISVLLRHSETKLIFVDQA 120
           I++GHVVSV+APN PAMYELHFA  M GA+LN INLRLDPRT+S+LLRHSE+KL+FVDQ 
Sbjct: 61  IKKGHVVSVLAPNIPAMYELHFAALMSGAILNNINLRLDPRTVSILLRHSESKLVFVDQL 120

Query: 121 SCALISDALALFPSETKRPLLVLIADDTAVQDSSLSSSVLIRNDFVDSYEEMVWKGDSNF 180
           S +LI +AL+LFP     P L+LIADDT    SS SSS     +FV SYE++V +GD  F
Sbjct: 121 SQSLIHEALSLFPPGIPIPRLILIADDTDEASSSPSSSSSPAVEFVCSYEDLVERGDPGF 180

Query: 181 EWIRPVSEWDPMVLNYTSGTTSSPKGVVHCHRGIFTVALDSLVEWGVPKQSVYLWTLPMF 240
           EWIRP SEWDP +LNYTSGTTSSPKGVVHC RG+F + +DSL++W VPKQ VYLWTLPMF
Sbjct: 181 EWIRPTSEWDPAILNYTSGTTSSPKGVVHCQRGLFLITVDSLIDWAVPKQPVYLWTLPMF 240

Query: 241 HANGWSFPWGIAAVGGTNVCIRKFDASLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNRPL 300
           HANGWSFPWG+AAVGGTN+C+RKFDA++I+ LIR H+VTH+CGAPVVLNML+NSPDN PL
Sbjct: 241 HANGWSFPWGMAAVGGTNICLRKFDATIIYRLIRAHNVTHMCGAPVVLNMLSNSPDNEPL 300

Query: 301 DRPVKILTAGAPPPAAVLLRIESLGFVVTHGYGLTESGGTIISCSWKSEWNRLTQTEQAR 360
             PV ILTAGAPPPAAVL R ESLGFVV+HGYGLTE+ G ++SC+WK EWNRL  +E+AR
Sbjct: 301 KNPVNILTAGAPPPAAVLHRTESLGFVVSHGYGLTETAGLVVSCAWKREWNRLPASERAR 360

Query: 361 LKARQGVRMLAVEAADVLDPKTRKSVKRDGASIGEIVVRGGSIMLGYLKDPEATAKAMTD 420
           LKARQGVR+  +   DVLDP T KSVKRDG+++GEIV+RGG +MLGYLKDP  TAK M  
Sbjct: 361 LKARQGVRLAGMTRIDVLDPNTGKSVKRDGSTLGEIVLRGGCVMLGYLKDPAGTAKCM-K 420

Query: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPVVNEVAVVARPD 480
           DGWFFTGDV VMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLYM+P +NE AVVARPD
Sbjct: 421 DGWFFTGDVAVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMHPAINEAAVVARPD 480

Query: 481 EFWGETPCAFVNLKKGLT-RQPTEEEIIEFCKGKLPRFMVPKTVVFTTELPKTSTGKILK 540
           +FWGETPCAFV+LK+ +   +P+E+EI+EFC+ KLP +MVPKTVVF  ELPKTSTGKI K
Sbjct: 481 DFWGETPCAFVSLKERIRGNKPSEKEIMEFCREKLPHYMVPKTVVFKEELPKTSTGKIQK 540

Query: 541 SVLREMAKSMGP--TPKSRL 558
            +LRE AKSMG   T +SR+
Sbjct: 541 FILRETAKSMGSAGTRRSRI 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AAE5_ARATH1.4e-21865.77Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 P... [more]
AAE6_ARATH2.0e-21264.25Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1[more]
AAE8_ARATH6.8e-20563.52Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana GN=AAE8 PE=2 SV=1[more]
AEE9_ARATH7.0e-20261.04Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1[more]
AAE4_ARATH2.7e-19859.71Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana GN=AEE4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KMT3_CUCSA6.6e-28486.00AMP dependent ligase OS=Cucumis sativus GN=Csa_5G318390 PE=4 SV=1[more]
A0A0A0KT33_CUCSA1.5e-26481.33AMP dependent ligase OS=Cucumis sativus GN=Csa_5G317890 PE=4 SV=1[more]
B9S6X4_RICCO1.6e-23770.61AMP dependent ligase, putative OS=Ricinus communis GN=RCOM_1328840 PE=4 SV=1[more]
A0A061ESR0_THECC1.4e-23671.27Acyl activating enzyme 5 OS=Theobroma cacao GN=TCM_020359 PE=4 SV=1[more]
A0A068U0B9_COFCA4.4e-23569.05Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00036869001 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G16370.17.9e-22065.77 acyl activating enzyme 5[more]
AT5G16340.11.1e-21364.25 AMP-dependent synthetase and ligase family protein[more]
AT1G75960.13.8e-20663.52 AMP-dependent synthetase and ligase family protein[more]
AT1G21540.13.9e-20361.04 AMP-dependent synthetase and ligase family protein[more]
AT1G77240.11.5e-19959.71 AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
gi|659126420|ref|XP_008463174.1|6.4e-28887.25PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo][more]
gi|449462358|ref|XP_004148908.1|9.5e-28486.00PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus][more]
gi|449462360|ref|XP_004148909.1|2.2e-26481.33PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus][more]
gi|659126423|ref|XP_008463175.1|1.6e-26280.07PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo][more]
gi|1009151390|ref|XP_015893528.1|3.8e-24071.96PREDICTED: probable acyl-activating enzyme 6 [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006744 ubiquinone biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003987 acetate-CoA ligase activity
molecular_function GO:0008756 o-succinylbenzoate-CoA ligase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG01G008380.1ClCG01G008380.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 24..450
score: 2.9
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205
scor
IPR025110AMP-binding enzyme C-terminal domainPFAMPF13193AMP-binding_Ccoord: 459..536
score: 2.9
NoneNo IPR availableGENE3DG3DSA:2.30.38.10coord: 375..440
score: 2.2
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 447..549
score: 2.3
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 179..203
score: 1.9E-29coord: 15..127
score: 1.9E-29coord: 204..349
score: 5.1
NoneNo IPR availablePANTHERPTHR24095FAMILY NOT NAMEDcoord: 1..557
score:
NoneNo IPR availablePANTHERPTHR24095:SF177ACYL-ACTIVATING ENZYME 5, PEROXISOMAL-RELATEDcoord: 1..557
score:
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 11..548
score: 7.98E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None