Carg27775 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg27775
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	rRNA N-glycosidase
Location	Cucurbita_argyrosperma_scaffold_363 : 69187 .. 70104 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCATTGGATCAACTCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGTTCCTCGTCCTTCCGCCAATGCTAATGTCAGCTTCTCTCTGTTGGGTGCCACGGCCGAAACCTACAAACAATTCATTAAAGATCTGCGCAAGGCACTTACGGTAAATTCCCACAAAGTGTATGGCATTCCTGTGTTGCCAGCCACAGCCAAAGGGTTGGCACGCTTCATATTGGTCAATCTCACCAATTACAATGGTGAATCCATCACGGTTGCAATTGACACAGTGGATGTTTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCACATAATGTGCTATTTAAAGGTAGCAAACATGAAACACTTTGGTATAAAGGGAATTATGATGACCTTGAGAGGCATGCAGGTAAGATTTCGAGAGAGAATGTTCAGCTTGGGTTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCATTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAATATATTGAGAAAATAAATTCTGACAACGTTAGTTCAACGTTTAAACCAGATCCTGGATACTTGAGTTTAGAAAACCGTTGGAACAATCTGTCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATGCAGTTCAAGTTCGAAGTGTTGGCAATAAATCAATTAAGGTAACTGATGTTAATTCGCCAGTTGTTAAAGGAATTGCGCTGTTACTTTGCAACAAGCCAGGGGCTAATGGAGTATCCAAACTGATGGAAGAGTTTATCCATTGGGAAGCAGCTCGAGGTGATTTTGGAATTGATTTCTGA

mRNA sequence

ATGGAGTGCATTGGATCAACTCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGTTCCTCGTCCTTCCGCCAATGCTAATGTCAGCTTCTCTCTGTTGGGTGCCACGGCCGAAACCTACAAACAATTCATTAAAGATCTGCGCAAGGCACTTACGGTAAATTCCCACAAAGTGTATGGCATTCCTGTGTTGCCAGCCACAGCCAAAGGGTTGGCACGCTTCATATTGGTCAATCTCACCAATTACAATGGTGAATCCATCACGGTTGCAATTGACACAGTGGATGTTTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCACATAATGTGCTATTTAAAGGTAGCAAACATGAAACACTTTGGTATAAAGGGAATTATGATGACCTTGAGAGGCATGCAGGTAAGATTTCGAGAGAGAATGTTCAGCTTGGGTTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCATTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAATATATTGAGAAAATAAATTCTGACAACGTTAGTTCAACGTTTAAACCAGATCCTGGATACTTGAGTTTAGAAAACCGTTGGAACAATCTGTCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATGCAGTTCAAGTTCGAAGTGTTGGCAATAAATCAATTAAGGTAACTGATGTTAATTCGCCAGTTGTTAAAGGAATTGCGCTGTTACTTTGCAACAAGCCAGGGGCTAATGGAGTATCCAAACTGATGGAAGAGTTTATCCATTGGGAAGCAGCTCGAGGTGATTTTGGAATTGATTTCTGA

Coding sequence (CDS)

ATGGAGTGCATTGGATCAACTCAAGACTTGATACAAGCTCCCTTTCCCTTAAACTGTGGTGCTCATGCTGCCATTCGTGTTCCTCGTCCTTCCGCCAATGCTAATGTCAGCTTCTCTCTGTTGGGTGCCACGGCCGAAACCTACAAACAATTCATTAAAGATCTGCGCAAGGCACTTACGGTAAATTCCCACAAAGTGTATGGCATTCCTGTGTTGCCAGCCACAGCCAAAGGGTTGGCACGCTTCATATTGGTCAATCTCACCAATTACAATGGTGAATCCATCACGGTTGCAATTGACACAGTGGATGTTTATATTGTCGCATACCAAGCAGGAAACAAGGCTTATTTTCTAAAAGATGTTTCCAAAGAAGCACATAATGTGCTATTTAAAGGTAGCAAACATGAAACACTTTGGTATAAAGGGAATTATGATGACCTTGAGAGGCATGCAGGTAAGATTTCGAGAGAGAATGTTCAGCTTGGGTTCTCTGAATTAAGCAGCTCCATTGGCAACATGTTCCATTATAACGCTGGCTATTCTGTGCCTAGATCGTTTATTGTGATGATTCAAATGCTTTCAGAAGCTTCGAGGTTTAAATATATTGAGAAAATAAATTCTGACAACGTTAGTTCAACGTTTAAACCAGATCCTGGATACTTGAGTTTAGAAAACCGTTGGAACAATCTGTCTGAACAGGTACAGAAGGCGCAAAAGCATGGAGGTAAATTTGAGCATGCAGTTCAAGTTCGAAGTGTTGGCAATAAATCAATTAAGGTAACTGATGTTAATTCGCCAGTTGTTAAAGGAATTGCGCTGTTACTTTGCAACAAGCCAGGGGCTAATGGAGTATCCAAACTGATGGAAGAGTTTATCCATTGGGAAGCAGCTCGAGGTGATTTTGGAATTGATTTCTGA

Protein sequence

MECIGSTQDLIQAPFPLNCGAHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNKPGANGVSKLMEEFIHWEAARGDFGIDF

Homology

[Close]

BLAST of Carg27775 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.4 bits (1435), Expect = 3.1e-155
Identity = 278/305 (91.15%), Postives = 286/305 (93.77%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIR PRPSANAN+SFSLLGATA+TYKQFI DLR ALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRNALT 60

Query: 61  VNSHKVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           V SHKVYGIPVLPATAKGLARFILVNLTNY GESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VQSHKVYGIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGY 180
           VSKEAH+VLFKGSKHETL YKGNYDDLERHAG ISREN++LGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIE  N + V S FKPDPGYLSLENRW+NLSEQVQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNKPGANGVSKLMEEFIHWEAARGD 300
           GGKFE  VQVRSV NK IKVTDVNSPVVKGIALLLCNKPG NGVSKLME+FIHWEAARG 
Sbjct: 241 GGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNGVSKLMEQFIHWEAARGA 300

Query: 301 FGIDF 306
           FGIDF
Sbjct: 301 FGIDF 305

[Close]

BLAST of Carg27775 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 555.1 bits (1429), Expect = 1.5e-154
Identity = 277/305 (90.82%), Postives = 286/305 (93.77%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALT 60
           MECIGSTQDLIQAPFPLNCGAHAAIR PRPSANAN+SFSLLGATA+TYKQFI DLRKALT
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANANLSFSLLGATAQTYKQFINDLRKALT 60

Query: 61  VNSHKVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVY IPVLPATAKGLARFILVNLTNY GESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGY 180
           VSKEAH+VLFKGSKHETL YKGNYDDLERHAGKISREN++LGFSELSSSIGNMFHY AGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYKAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKH 240
           SVPRSFIVMIQMLSEASRFKYIE  N + V S FKPDPGYLSLENRW+NLSEQVQKAQKH
Sbjct: 181 SVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNKPGANGVSKLMEEFIHWEAARGD 300
           GGKFEH VQVRSV NK + V+DVNSPVVKGIALLL NKPG NGVSKLMEEFIHWEAARG 
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVSKLMEEFIHWEAARGH 300

Query: 301 FGIDF 306
           FGIDF
Sbjct: 301 FGIDF 305

[Close]

BLAST of Carg27775 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 553.9 bits (1426), Expect = 3.4e-154
Identity = 275/305 (90.16%), Postives = 286/305 (93.77%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLNCGAHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALT 60
           MECIGST+DLIQAPFPLNCGAH AIR PRPSANAN+SFSLLGATA+TYKQFI DLRKALT
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRAPRPSANANLSFSLLGATAQTYKQFISDLRKALT 60

Query: 61  VNSHKVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKD 120
           VNSHKVY IPVLPATAKGLARFILVNLTNY GESITVAIDTVDVYIVAYQAGNKAYFLKD
Sbjct: 61  VNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYFLKD 120

Query: 121 VSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGY 180
           VSKEAH+VLFKGSKHETL YKGNYDDLERHAGKISREN+ LGFSELSSSIGNMFHYNAGY
Sbjct: 121 VSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYNAGY 180

Query: 181 SVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKH 240
           SVP+SFIVMIQMLSEASRFKYIE  N +NV S FKPDPGYLSLENRW+NLSEQVQKAQKH
Sbjct: 181 SVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKH 240

Query: 241 GGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNKPGANGVSKLMEEFIHWEAARGD 300
           GGKFEH VQVRSV NK + V+DVNSPVVKG+ALLLCNKPG NGVSKLMEEFI WEAARG 
Sbjct: 241 GGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVSKLMEEFIKWEAARGH 300

Query: 301 FGIDF 306
           FGIDF
Sbjct: 301 FGIDF 305

[Close]

BLAST of Carg27775 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 374.0 bits (959), Expect = 4.8e-100
Identity = 205/311 (65.92%), Postives = 238/311 (76.53%), Query Frame = 0


Query: 1   MECIGSTQDLIQAPFPLN----CGAHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLR 60
           MEC+ S++  IQ P P N     G HA   V  P  N+NVSFSLLGAT +TY+QFI++LR
Sbjct: 1   MECVRSSEPFIQ-PLPFNDIGIHGVHANGAVNAP--NSNVSFSLLGATGKTYQQFIQNLR 60

Query: 61  KALTVNSHKVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAY 120
            ALT+NS  VY IPVL ATA   ARFILV+LTNY  E+ITVAID V+VYIVAYQAGNKAY
Sbjct: 61  NALTINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAY 120

Query: 121 FLKDVSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHY 180
           FLKD SKEA +VLFKG K E L YKGNYD LE  AGKISRE + LGFSEL S+IGNM+HY
Sbjct: 121 FLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHY 180

Query: 181 NAGYSVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQK 240
           NAG SVPR+FIVMIQ +SEA+RFKYIE   S NV + FKPDPG+LSLENRW++LSEQVQ 
Sbjct: 181 NAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQI 240

Query: 241 AQKHGGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALLLCNKPGANG-VSKLMEEF-IHW 300
           AQ   G+F   ++VRSV NK I VT+V S VV+G+ALLL +KP  NG + +LMEEF   W
Sbjct: 241 AQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMELMEEFNKEW 300

Query: 301 EAARGDFGIDF 306
           E  RG+FGIDF
Sbjct: 301 ENGRGEFGIDF 308

[Close]

BLAST of Carg27775 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 329.3 bits (843), Expect = 1.4e-86
Identity = 175/262 (66.79%), Postives = 211/262 (80.53%), Query Frame = 0


Query: 31  SANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFILVNLTNY 90
           +A A+VSFS+LG+T+ETYKQFIK++R ALTV S  VY IPVL ATA G ARF+LV+LTNY
Sbjct: 18  AAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTNY 77

Query: 91  NGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGNYDDLERH 150
           N ESI VAID V+VY+VAY+AGN AYFL D S EA  VLF+G  H  L YKGNYD LE  
Sbjct: 78  NSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGLET- 137

Query: 151 AGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIEKINSDNV 210
           A KISRE + LGFSE+SSSIGNMFH+NAG SVPR+FIV+IQ +SEA+RFKYIE+  S+NV
Sbjct: 138 AAKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSENV 197

Query: 211 SSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDVNSPVVKG 270
            +TFKPDP +LSL+NRW++LSEQ+Q AQK GG+F  AV++R+V NK I VTDV+SPVVKG
Sbjct: 198 KTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVKG 257

Query: 271 IALLLCNKPGANGVSKLMEEFI 293
           IALLL  K     VS +M++ I
Sbjct: 258 IALLLYYK-----VSVVMDDNI 273

[Close]

BLAST of Carg27775 vs. Swiss-Prot
Match: sp|P98184|RIP2_BRYDI (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 5.8e-81
Identity = 158/259 (61.00%), Postives = 196/259 (75.68%), Query Frame = 0


Query: 17  LNCGAHAAIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATA 76
           L  GAH        + + +++FSL+GAT  TYK FI++LR  LTV + +VY IPVL   A
Sbjct: 12  LYVGAHV-------TEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAA 71

Query: 77  KGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHE 136
            GLARF LV LTNYNGES+TVA+D V+VY+VAY+AGN AYFL D S EA+NVLF G  H 
Sbjct: 72  AGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHV 131

Query: 137 TLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEA 196
            L Y GNYD LE  AG+ISREN++LGFSE+SS+IGNMF +N G SVPR+FIV+IQ +SEA
Sbjct: 132 RLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEA 191

Query: 197 SRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNK 256
           +RFKYIE+  S+NV + FKPDP +LSL+N W +LSEQ+Q AQ  GG+F   V++R+V N 
Sbjct: 192 ARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNT 251

Query: 257 SIKVTDVNSPVVKGIALLL 276
              VT+VNSPVVKGIALLL
Sbjct: 252 PTFVTNVNSPVVKGIALLL 263

[Close]

BLAST of Carg27775 vs. Swiss-Prot
Match: sp|P24817|RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 2.7e-54
Identity = 121/254 (47.64%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           AI +  P+A  +V+F L  ATA+TY +FI+D R  L   SHKVY IP+L +T     RFI
Sbjct: 13  AIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFI 72

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
           L+NLT+Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+N+LFKG++  TL Y GN
Sbjct: 73  LLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGN 132

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           Y++L+  A KI REN+ LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE
Sbjct: 133 YENLQTAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIE 192

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDV 263
           +  +  V++ FKP+   +SLEN+W+ LS+Q+  AQ  GGKF + V +     +  +VT+V
Sbjct: 193 RHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNV 252

Query: 264 NSPVVKGIALLLCN 278
           +S VVKG   LL N
Sbjct: 253 DSDVVKGNIKLLLN 263

[Close]

BLAST of Carg27775 vs. Swiss-Prot
Match: sp|P29339|RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.0e-53
Identity = 120/254 (47.24%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           AI +  P+A  +V+F L  ATA+TY +FI+D R  L   SHKVY IP+L +T     RFI
Sbjct: 13  AIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFI 72

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
           L++LT+Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+N+LFKG++  TL Y GN
Sbjct: 73  LLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGN 132

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           Y++L+  A KI REN+ LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE
Sbjct: 133 YENLQTAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIE 192

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDV 263
           +  +  V++ FKP+   +SLEN+W+ LS+Q+  AQ  GGKF + V +     +  +VT+V
Sbjct: 193 RHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNV 252

Query: 264 NSPVVKGIALLLCN 278
           +S VVKG   LL N
Sbjct: 253 DSDVVKGNIKLLLN 263

[Close]

BLAST of Carg27775 vs. Swiss-Prot
Match: sp|P84531|RIP_CUCMO (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.2e-49
Identity = 113/242 (46.69%), Postives = 160/242 (66.12%), Query Frame = 0


Query: 35  NVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFILVNLTNYNGES 94
           NV F L  AT+ +YK FIK+LR+AL  +  KVY IPVL +T     RFIL++L NY+G+S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 95  ITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKI 154
           IT AID ++VYIVAY  G  +YF + V  +A  +LFKG++  TL Y GNY++L+  A K+
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL 120

Query: 155 SRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTF 214
            REN++LG   L S+I  +FHYNA  +   + +V+IQ  SEA+RF+YIE   ++NV + F
Sbjct: 121 -RENIELGLPALDSAITTLFHYNA-EAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 215 KPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDVNSPVV-KGIAL 274
           KP    +SLEN W+ LS+Q+Q A+   G+FE  V +       +++T+V S VV + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 239

Query: 275 LL 276
           LL
Sbjct: 241 LL 239

[Close]

BLAST of Carg27775 vs. Swiss-Prot
Match: sp|P56626|RIP1_TRIAN (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 7.2e-47
Identity = 114/272 (41.91%), Postives = 164/272 (60.29%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           AI +  P+A  +VSF L  AT ++Y  FI  LR AL      V GIP+LP+TA G   F 
Sbjct: 9   AISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQG-TVCGIPLLPSTASGSQWFR 68

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
             NLTNYN E++TVA++  +VYIVAY+A   +YF +D   EA  ++F G+K   L Y GN
Sbjct: 69  FFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTVKLPYSGN 128

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           YD L+   GK  R+ ++LG   LSS+I NM +Y+   S   + +V+IQ  +EA+R+KYIE
Sbjct: 129 YDKLQSVVGK-QRDMIELGIPALSSAITNMVYYDY-QSTAAALLVLIQCTAEAARYKYIE 188

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHG-GKFEHAVQVRSVGNKSIKVTD 263
           +  S ++SS F P+   +SLEN+W  LS+Q+Q A + G G+FE+ V++ +       VT+
Sbjct: 189 QQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTRFSVTN 248

Query: 264 VNSPVVKG-IALLLCNKPGANGVSKLMEEFIH 294
            ++ VVKG I LLL  K        +    +H
Sbjct: 249 TSAGVVKGNIKLLLYYKASVGSEYDIPTTILH 277

[Close]

BLAST of Carg27775 vs. TrEMBL
Match: tr|B1B626|B1B626_TRIKI (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.5e-81
Identity = 161/255 (63.14%), Postives = 198/255 (77.65%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           A+ V      A+V+FSLLG+T+ TYKQFI++LR ALTV S  V  IPVL  TA GLARF+
Sbjct: 11  ALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGTAAGLARFL 70

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
           LV+LTNYNGESITVA+D V+VY+VAY+AGN AYFL D S EA+NVLFKG  H  L Y GN
Sbjct: 71  LVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINHVRLPYGGN 130

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           YD LE  AG+ISRE + LGFSE+SS+IG+MFHYNAG SVP++FIV+IQ +SEA+RFKYI+
Sbjct: 131 YDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTVSEAARFKYIQ 190

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDV 263
           +  S+NV + F PDP +LSLEN W  LSEQ+Q AQ  GG+F   +++R+V N    VT+V
Sbjct: 191 QRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTVSNTPTFVTNV 250

Query: 264 NSPVVKGIALLLCNK 279
           NSPVVKG+ALLL  K
Sbjct: 251 NSPVVKGVALLLYYK 265

[Close]

BLAST of Carg27775 vs. TrEMBL
Match: tr|S6CNA0|S6CNA0_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 8.5e-53
Identity = 122/254 (48.03%), Postives = 173/254 (68.11%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           AI +  P+A  +V+F L  ATA+TY +FI+D R  L   SHKVY IP+L +T  G  RFI
Sbjct: 7   AIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISGSRRFI 66

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
           L+NLT+Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+N+LFKG++  TL Y GN
Sbjct: 67  LLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGN 126

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           Y++L+  A KI REN+ LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE
Sbjct: 127 YENLQTAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIE 186

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDV 263
           +  +  V++ FKP+   +SLEN+W+ LS+Q+  AQ  GGKF + V +     +  +VT+V
Sbjct: 187 RHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNV 246

Query: 264 NSPVVKGIALLLCN 278
           +S VVKG   LL N
Sbjct: 247 DSDVVKGNIKLLLN 257

[Close]

BLAST of Carg27775 vs. TrEMBL
Match: tr|S6CNH6|S6CNH6_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-52
Identity = 122/254 (48.03%), Postives = 173/254 (68.11%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           AI +  P+A  +V+F L  ATA+TY +FI+D R  L   SHKVY IP+L +T     RFI
Sbjct: 7   AIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFI 66

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
           L+NLT+Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+N+LFKG++  TL Y GN
Sbjct: 67  LLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGN 126

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           Y++L+  A KI REN+ LGF  LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE
Sbjct: 127 YENLQTAAHKI-RENIDLGFPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIE 186

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDV 263
           +  +  V++ FKP+   +SLEN+W+ LS+Q+  AQ  GGKF + V +     +  +VT+V
Sbjct: 187 RHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNV 246

Query: 264 NSPVVKGIALLLCN 278
           +S VVKG   LL N
Sbjct: 247 DSDVVKGNIKLLLN 257

[Close]

BLAST of Carg27775 vs. TrEMBL
Match: tr|S6CNY1|S6CNY1_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 5.5e-52
Identity = 121/254 (47.64%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           AI +  P+A  +V+F L  ATA+TY +FI+D R  L   SHKVY IP+L +T     RFI
Sbjct: 7   AIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFI 66

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
           L+NLT+Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+N+LFKG++  TL Y GN
Sbjct: 67  LLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGN 126

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           Y++L+  A KI REN+ LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE
Sbjct: 127 YENLQTAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIE 186

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDV 263
           +  +  V++ FKP+   +SLEN+W+ LS+Q+  AQ  GGKF + V +     +  +VT+V
Sbjct: 187 RHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNV 246

Query: 264 NSPVVKGIALLLCN 278
           +S VVKG   LL N
Sbjct: 247 DSDVVKGNIKLLLN 257

[Close]

BLAST of Carg27775 vs. TrEMBL
Match: tr|Q0ZBB9|Q0ZBB9_MOMCH (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 5.5e-52
Identity = 121/254 (47.64%), Postives = 172/254 (67.72%), Query Frame = 0


Query: 24  AIRVPRPSANANVSFSLLGATAETYKQFIKDLRKALTVNSHKVYGIPVLPATAKGLARFI 83
           AI +  P+A  +V+F L  ATA+TY +FI+D R  L   SHKVY IP+L +T     RFI
Sbjct: 13  AIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFI 72

Query: 84  LVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHNVLFKGSKHETLWYKGN 143
           L+NLT+Y  E+I+VAID  +VY+VAY+  + +YF K+   EA+N+LFKG++  TL Y GN
Sbjct: 73  LLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGN 132

Query: 144 YDDLERHAGKISRENVQLGFSELSSSIGNMFHYNAGYSVPRSFIVMIQMLSEASRFKYIE 203
           Y++L+  A KI REN+ LG   LSS+I  +F+YNA  S P + +V+IQ  +EA+RFKYIE
Sbjct: 133 YENLQTAAHKI-RENIDLGLPALSSAITTLFYYNA-QSAPSALLVLIQTTAEAARFKYIE 192

Query: 204 KINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQKHGGKFEHAVQVRSVGNKSIKVTDV 263
           +  +  V++ FKP+   +SLEN+W+ LS+Q+  AQ  GGKF + V +     +  +VT+V
Sbjct: 193 RHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNV 252

Query: 264 NSPVVKGIALLLCN 278
           +S VVKG   LL N
Sbjct: 253 DSDVVKGNIKLLLN 263

The following BLAST results are available for this feature:

BLAST of Carg27775 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523582.1	3.1e-155	91.15	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]
XP_022926255.1	1.5e-154	90.82	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
XP_022981562.1	3.4e-154	90.16	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]
XP_022158191.1	4.8e-100	65.92	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158193.1	1.4e-86	66.79	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of Carg27775 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Carg27775 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P98184\|RIP2_BRYDI	5.8e-81	61.00	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
sp\|P24817\|RIP3_MOMCH	2.7e-54	47.64	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
sp\|P29339\|RIP2_MOMBA	1.0e-53	47.24	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
sp\|P84531\|RIP_CUCMO	1.2e-49	46.69	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
sp\|P56626\|RIP1_TRIAN	7.2e-47	41.91	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]

BLAST of Carg27775 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|B1B626\|B1B626_TRIKI	2.5e-81	63.14	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
tr\|S6CNA0\|S6CNA0_MOMCH	8.5e-53	48.03	Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P...	[more]
tr\|S6CNH6\|S6CNH6_MOMCH	1.1e-52	48.03	Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P...	[more]
tr\|S6CNY1\|S6CNY1_MOMCH	5.5e-52	47.64	Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P...	[more]
tr\|Q0ZBB9\|Q0ZBB9_MOMCH	5.5e-52	47.64	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0017148	negative regulation of translation

Vocabulary: **Molecular Function**
Term	Definition
GO:0030598	rRNA N-glycosylase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036041	Ribosome-inact_prot_sf
IPR016138	Ribosome_inactivat_prot_sub1
IPR016139	Ribosome_inactivat_prot_sub2
IPR001574	Ribosome_inactivat_prot
IPR017989	Ribosome_inactivat_1/2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0030598	rRNA N-glycosylase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27775-RA	Carg27775-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 220..240
None	No IPR available	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 44..249
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 46..59 score: 41.73 coord: 186..206 score: 52.63 coord: 94..109 score: 44.9 coord: 156..170 score: 44.21 coord: 218..235 score: 40.94
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 44..235 e-value: 2.4E-33 score: 115.3
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	G3DSA:4.10.470.10		coord: 198..281 e-value: 2.1E-22 score: 80.8
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	G3DSA:3.40.420.10		coord: 34..197 e-value: 9.0E-52 score: 177.0
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	SSF56371	Ribosome inactivating proteins (RIP)	coord: 36..276

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg27775	Cucurbita maxima (Rimu)	carcmaB0923
Carg27775	Cucurbita moschata (Rifu)	carcmoB0897
Carg27775	Wild cucumber (PI 183967)	carcpiB0684
Carg27775	Cucumber (Chinese Long) v2	carcuB0674
Carg27775	Cucumber (Chinese Long) v3	carcucB0686
Carg27775	Bottle gourd (USVL1VR-Ls)	carlsiB594
Carg27775	Watermelon (97103) v1	carwmB0651
Carg27775	Watermelon (Charleston Gray)	carwcgB0634