Carg27606 (gene) Silver-seed gourd

NameCarg27606
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
LocationCucurbita_argyrosperma_scaffold_314 : 40542 .. 42793 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTTTATGAGTGGATAAGCCATTGCCGATTCCGTTGTATCATTTGCTGATCCTTCATCATCTTCTTCTTCTTCTCTCGCTTCGCTAGTTTAACCTCCGCTCTGCAATTTCCAGCCACTATTAAGGTGGTTGTGGCGGACTCTGATTCCGATTAGATTCGGAGAAGAAAGGGGGAGAATCTCCGGAGTTGCAATGGCTGTGCAGGATCATGGAGCTTTCATGGAGAAATTCCTCCTGCAACCGAGCTCCCCGTCCGATCAACTTCCCTTGGCTGGCCTCACTTTCGCTGTTAAAGACATGTGAGATTTTTTCTTTTTGATTATTTTCTACTTTTTATTTGGCTTGATAACAGGTTATGAGTTCTGTAATTTCTCTAAAGTATGATGGATAGTATGAAACGCTTTCAGTTTCAATGGTTGGTGGAATTGATGATTCCATCCGATATTATTTATTCGATCAACATATATTCATGAAATGAATTGATCGTTCTTTGGGGAAAATTCCGTTTGTACTAGTGAGTCGATACATTAATTAGTAGTAAGATCAAATTACGAAGTTTTCCATGAATCAATCAATGCATTAAGAAAATAAAACAAGAAGAAATTTAACCAAAAAATGCTAAAAGTTAAGCATCATAATTGTCTAAAACCAATCACAAAAGGTACTTGAATCTAACTACAAAGTAAAGCAAGTGACGAAGTTTCCAAGCTTAACAATAACATGTCCCAACCATGTTAAAGCATCCAATATAACCGGGAAAATGGGATTCATAAAGCATCCATAACTGCTCCTAACTTTCGATGAAGTAAAAGGTAACTCATCTTATATCTTTTAAGAACATTACATCAGGCATGTGGCGGGGAGTTCAACTCTAAGGTTCAAACCTACAACCTCCTGGAGAAAGTAGAGCTGTTTTAATTCTTCAACTTATAGAGAGTATTGATTTTACCACTTAACTAATTTTAGGGTTGAATTTGATGAAATTAATACTTTAAAGGCTAAATTGTACTCACCCATTTTAATACAAGGGTAAAATAATTTTCTGTTGTGAAGTTCTATTTGAATTCTTCTTTTAGAGTAGAAAGAGTATTATGAGTAGTGAAATGAAGATTAAATTGTTATATAACATAAGATTATCGTAACGTTTGTCGCTAAAGCCTAATTCTTATGGCCGATATATTTGTAGATATTGATATCTCCATTCTTACAAGCCATCACCAGTTGGTTTTCTGTTTTAACTGTTCATTAGTGTGTGTAAGATGAAGCTTAGTTCAATAAAATAATTCTTTGTTGTTTTAATGCATGGTCCTTCATATAAAAGTGTCAGTTTTTTTTTTCATGAAACCGGGGCGTTGATGAAAAACTTCTCATTGGACTTTGAACCAAACAGATTTGATGTGGATGGATATGTAACTGGTTTTGGAAATCCTGAATGGCTAAGGACACATCCACCTGCTAGTCAGACAGCCCCTGCCGTGTTGTCGATCCTAAAAGGAGGAGCCACCTGCATTGGCAAGACTATCATGGATGAAATGGCCTACAGGTACCAAGAATTTCTAGCTTTTACTCTTTCTTTTCCTTTTAGCTTTCATTTTTTACAATTGATAACAAACAAACAAGGCATAAGCTTTGGAAATTGGGATACAGCAGATAAAACCATTTTGCACTGCACACCATTCTGTAATTGTCTTAAAACTATGTCTACTCCATTCTTATCATTCTATTGCAAGCAGACAACTCTGCATTGTAATTGGTCAACATATGAACTGAACCAATTATCTTTTGACAGGTTTCTAATTTCTAGTTGAAAATCTTTCACCAAATGATTGTTTGTGACGATGTAGGCTTTTGAATATTTTCATTAGTGGAGTCACTAGCTTGATAATCATATTTTTTCAGTATAAATGGGGAAAACATTCACTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGCTCTGCCGTTGCTGTAGGTGCAAAGCTTGTGGATTTCTCCTTAGGCAAGTTCCTAGTTCTTTGACCTTGAGCAAATTGTGGTTTAATGAATTCTCCCTGGAATATGTTGTAAAGGTCATATTTAGTTTTGTAGGAACTGATACTGGGGGTAGTGTAAGAGTGCCTGCATCCTATTGTGGAATCTTTGGATTTCGGCCTTCACATGGTGCAGTCTCTACCTCTGGAGTAATTCCCATGGCACAGAGCTTTGATACAGTAGGTAATGAGTAG

mRNA sequence

ATTTTTTTATGAGTGGATAAGCCATTGCCGATTCCGTTGTATCATTTGCTGATCCTTCATCATCTTCTTCTTCTTCTCTCGCTTCGCTAGTTTAACCTCCGCTCTGCAATTTCCAGCCACTATTAAGGTGGTTGTGGCGGACTCTGATTCCGATTAGATTCGGAGAAGAAAGGGGGAGAATCTCCGGAGTTGCAATGGCTGTGCAGGATCATGGAGCTTTCATGGAGAAATTCCTCCTGCAACCGAGCTCCCCGTCCGATCAACTTCCCTTGGCTGGCCTCACTTTCGCTGTTAAAGACATATTTGATGTGGATGGATATGTAACTGGTTTTGGAAATCCTGAATGGCTAAGGACACATCCACCTGCTAGTCAGACAGCCCCTGCCGTGTTGTCGATCCTAAAAGGAGGAGCCACCTGCATTGGCAAGACTATCATGGATGAAATGGCCTACAGGCTTTTGAATATTTTCATTAGTGGAGTCACTAGCTTGATAATCATATTTTTTCAGTATAAATGGGGAAAACATTCACTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGCTCTGCCGTTGCTGTAGGTGCAAAGCTTGTGGATTTCTCCTTAGGCAAGTTCCTAGTTCTTTGACCTTGAGCAAATTGTGGTTTAATGAATTCTCCCTGGAATATGTTGTAAAGGTCATATTTAGTTTTGTAGGAACTGATACTGGGGGTAGTGTAAGAGTGCCTGCATCCTATTGTGGAATCTTTGGATTTCGGCCTTCACATGGTGCAGTCTCTACCTCTGGAGTAATTCCCATGGCACAGAGCTTTGATACAGTAGGTAATGAGTAG

Coding sequence (CDS)

ATGGCTGTGCAGGATCATGGAGCTTTCATGGAGAAATTCCTCCTGCAACCGAGCTCCCCGTCCGATCAACTTCCCTTGGCTGGCCTCACTTTCGCTGTTAAAGACATATTTGATGTGGATGGATATGTAACTGGTTTTGGAAATCCTGAATGGCTAAGGACACATCCACCTGCTAGTCAGACAGCCCCTGCCGTGTTGTCGATCCTAAAAGGAGGAGCCACCTGCATTGGCAAGACTATCATGGATGAAATGGCCTACAGGCTTTTGAATATTTTCATTAGTGGAGTCACTAGCTTGATAATCATATTTTTTCAGTATAAATGGGGAAAACATTCACTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGCTCTGCCGTTGCTGTAGGTGCAAAGCTTGTGGATTTCTCCTTAGGCAAGTTCCTAGTTCTTTGACCTTGAGCAAATTGTGGTTTAATGAATTCTCCCTGGAATATGTTGTAAAGGTCATATTTAGTTTTGTAGGAACTGATACTGGGGGTAGTGTAAGAGTGCCTGCATCCTATTGTGGAATCTTTGGATTTCGGCCTTCACATGGTGCAGTCTCTACCTCTGGAGTAATTCCCATGGCACAGAGCTTTGATACAGTAGGTAATGAGTAG

Protein sequence

MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQTAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPTCIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDTGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVGNE
BLAST of Carg27606 vs. NCBI nr
Match: XP_022961591.1 (amidase 1-like [Cucurbita moschata])

HSP 1 Score: 245.7 bits (626), Expect = 1.4e-61
Identity = 136/219 (62.10%), Postives = 149/219 (68.04%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ
Sbjct: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAPAVLSILKGGATCIGKTIMDEMAY +                    G++  + TP+  
Sbjct: 61  TAPAVLSILKGGATCIGKTIMDEMAYSIN-------------------GENIHYGTPQ-N 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
              SD                      ++P   +         ++    K++   +GTDT
Sbjct: 121 PHASD----------------------RIPGGSSSGS------AVAVGAKLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG
Sbjct: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 171

BLAST of Carg27606 vs. NCBI nr
Match: XP_023516592.1 (amidase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.0 bits (624), Expect = 2.4e-61
Identity = 134/219 (61.19%), Postives = 147/219 (67.12%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ
Sbjct: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAPAVLSILKGGATCIGKTIMDEMAY +                    G++  + TP+  
Sbjct: 61  TAPAVLSILKGGATCIGKTIMDEMAYSIN-------------------GENIHYGTPQNP 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
                                      ++P   +         ++    K++   +GTDT
Sbjct: 121 RAPD-----------------------RIPGGSSSGS------AVAVGAKLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG
Sbjct: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 171

BLAST of Carg27606 vs. NCBI nr
Match: XP_022968725.1 (amidase 1 isoform X3 [Cucurbita maxima])

HSP 1 Score: 242.3 bits (617), Expect = 1.6e-60
Identity = 134/219 (61.19%), Postives = 148/219 (67.58%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPA+Q
Sbjct: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAPAV SILKGGATCIGKTIMDEMAY +                    G++  + TP+  
Sbjct: 61  TAPAVSSILKGGATCIGKTIMDEMAYSIN-------------------GENIHYGTPQ-N 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
              SD                      ++P   +         ++    K++   +GTDT
Sbjct: 121 PHASD----------------------RIPGGSSSGS------AVAVGAKLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG
Sbjct: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 171

BLAST of Carg27606 vs. NCBI nr
Match: XP_022968726.1 (amidase 1 isoform X4 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 5.6e-58
Identity = 130/219 (59.36%), Postives = 146/219 (66.67%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQDHGAF+EKF+LQ SSPSD LPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPA+Q
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAPAV SILKGGATCIGKTIMDEMAY +                    G++  + TP+  
Sbjct: 61  TAPAVSSILKGGATCIGKTIMDEMAYSIN-------------------GENIHYGTPQ-N 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
              SD                      ++P   +         ++    K++   +GTDT
Sbjct: 121 PHASD----------------------RIPGGSSSGS------AVAVGAKLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG
Sbjct: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 171

BLAST of Carg27606 vs. NCBI nr
Match: XP_022156705.1 (amidase 1 [Momordica charantia] >XP_022156710.1 amidase 1 [Momordica charantia])

HSP 1 Score: 229.9 bits (585), Expect = 8.1e-57
Identity = 121/219 (55.25%), Postives = 145/219 (66.21%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQDHGAF+EKFLLQPSSPSD+LPLAGLTFAVKDIFD+DGYVTGFGNPEW RTHPPA+Q
Sbjct: 1   MAVQDHGAFVEKFLLQPSSPSDELPLAGLTFAVKDIFDMDGYVTGFGNPEWARTHPPANQ 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAP V +IL+GGATCIG+T+MDEMAY +                    G++  + TP+  
Sbjct: 61  TAPTVSTILRGGATCIGRTVMDEMAYSIN-------------------GENIHYGTPQNP 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
                                  + L ++P   +         ++    K++   +GTDT
Sbjct: 121 -----------------------YALDRIPGGSSSGS------AVAVGAKLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGS+RVPASYCGI GFRPSHGAVSTSGVIPM+QSFDTVG
Sbjct: 181 GGSIRVPASYCGILGFRPSHGAVSTSGVIPMSQSFDTVG 171

BLAST of Carg27606 vs. TAIR10
Match: AT1G08980.1 (amidase 1)

HSP 1 Score: 174.5 bits (441), Expect = 7.3e-44
Identity = 99/216 (45.83%), Postives = 124/216 (57.41%), Query Frame = 0

Query: 5   DHGAFMEKFLLQPSSPSDQLP-LAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQTAP 64
           D GAF+EK  + P+S S   P L GLTFA+KDIFDV+G VTGFGNP+WLRTH  A+ TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  AVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPTCIR 124
            V S+L+ GAT +G TIMDEMAY +                    G+++ + TP+     
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSIN-------------------GENAHYGTPRNP--- 125

Query: 125 SDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDTGGS 184
                                   +VP   +         ++    +++   +GTDTGGS
Sbjct: 126 --------------------IAFDRVPGGSSSGS------AVAVAARLVDFSIGTDTGGS 173

Query: 185 VRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           VRVPASYCGIFGFRPSHGAVST G+ PMAQSFDTVG
Sbjct: 186 VRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVG 173

BLAST of Carg27606 vs. TAIR10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III)

HSP 1 Score: 149.1 bits (375), Expect = 3.3e-36
Identity = 81/220 (36.82%), Postives = 117/220 (53.18%), Query Frame = 0

Query: 4   QDHGAFMEKFLL----QPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPAS 63
           +D GAF++K +L    QP+ P    PL GLTFAV D+FD+ GYVTGFG+P+W+RTH  AS
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKP 123
            T+P V ++++GGATC+GKT++DE A+      ISG                   H   P
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFS-----ISGENK----------------HYDSP 151

Query: 124 TCIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTD 183
           T   + T                     ++P          +  ++      +   +G D
Sbjct: 152 TNPAAPT---------------------RIPGGAC------SGAAVAVATNAVDFALGID 203

Query: 184 TGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           T G VRVPA YCG+ GF+ S+GA+S +G+IP++ S D+VG
Sbjct: 212 TVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVG 203

BLAST of Carg27606 vs. TAIR10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V)

HSP 1 Score: 129.0 bits (323), Expect = 3.5e-30
Identity = 75/220 (34.09%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 4   QDHGAFMEKFLLQP----SSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPAS 63
           +D GAF+++F L P            L+GLTF++ D FDV  Y+TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPXXXXXXXXQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKP 123
           +TA  V ++LK GATC+GKTIMDE+ + ++                   G++  + TP  
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGII-------------------GENKHYGTPIN 164

Query: 124 TCIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTD 183
             +  +                       VP   +         ++    +++   +G D
Sbjct: 165 PLMPDN-----------------------VPGGCSSGS------AVSVGAELVDFSLGID 216

Query: 184 TGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           T G VRVPA++CGI GFRPS G VS+ GV+P +QS +TVG
Sbjct: 225 TTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVG 216

BLAST of Carg27606 vs. TAIR10
Match: AT3G25660.1 (Amidase family protein)

HSP 1 Score: 56.2 bits (134), Expect = 2.9e-08
Identity = 23/44 (52.27%), Postives = 33/44 (75.00%), Query Frame = 0

Query: 176 VGTDTGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           +G+DTGGSVR PAS+CG+ G +P++G VS  G++  A S D +G
Sbjct: 208 LGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIG 251

BLAST of Carg27606 vs. TAIR10
Match: AT5G07360.1 (Amidase family protein)

HSP 1 Score: 45.4 bits (106), Expect = 5.1e-05
Identity = 18/44 (40.91%), Postives = 31/44 (70.45%), Query Frame = 0

Query: 176 VGTDTGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           +G++T GS+  PA+ CGI   RP+ G+V  +GV+ +++S D +G
Sbjct: 365 IGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLG 408

BLAST of Carg27606 vs. Swiss-Prot
Match: sp|Q7XTK3|AMI1_ORYSJ (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 5.9e-43
Identity = 98/215 (45.58%), Postives = 121/215 (56.28%), Query Frame = 0

Query: 5   DHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQTAPA 64
           D+GAFME+F+L P  PS QLPL GLTFA+KDIFD+ G VTGFGNP+W RTH PA+ T+P 
Sbjct: 10  DYGAFMERFVL-PPPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPV 69

Query: 65  VLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPTCIRS 124
           VL+ L  GAT +G TIMDEMAY +                    G+++ + TP   C   
Sbjct: 70  VLAALAAGATSLGTTIMDEMAYSIN-------------------GENTHYGTPTNPCAPG 129

Query: 125 DTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDTGGSV 184
                                  +VP   +         ++     ++   +GTDTGGSV
Sbjct: 130 -----------------------RVPGGSSSGS------AVAVAANLVDFSLGTDTGGSV 175

Query: 185 RVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           RVPA+YCGIFG RPSHG VS   VIPMAQ FDTVG
Sbjct: 190 RVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVG 175

BLAST of Carg27606 vs. Swiss-Prot
Match: sp|Q9FR37|AMI1_ARATH (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.3e-42
Identity = 99/216 (45.83%), Postives = 124/216 (57.41%), Query Frame = 0

Query: 5   DHGAFMEKFLLQPSSPSDQLP-LAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQTAP 64
           D GAF+EK  + P+S S   P L GLTFA+KDIFDV+G VTGFGNP+WLRTH  A+ TAP
Sbjct: 6   DFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAP 65

Query: 65  AVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPTCIR 124
            V S+L+ GAT +G TIMDEMAY +                    G+++ + TP+     
Sbjct: 66  VVSSLLEAGATALGITIMDEMAYSIN-------------------GENAHYGTPRNP--- 125

Query: 125 SDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDTGGS 184
                                   +VP   +         ++    +++   +GTDTGGS
Sbjct: 126 --------------------IAFDRVPGGSSSGS------AVAVAARLVDFSIGTDTGGS 173

Query: 185 VRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           VRVPASYCGIFGFRPSHGAVST G+ PMAQSFDTVG
Sbjct: 186 VRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVG 173

BLAST of Carg27606 vs. Swiss-Prot
Match: sp|Q9LVH5|OE64C_ARATH (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 6.0e-35
Identity = 81/220 (36.82%), Postives = 117/220 (53.18%), Query Frame = 0

Query: 4   QDHGAFMEKFLL----QPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPAS 63
           +D GAF++K +L    QP+ P    PL GLTFAV D+FD+ GYVTGFG+P+W+RTH  AS
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKP 123
            T+P V ++++GGATC+GKT++DE A+      ISG                   H   P
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFS-----ISGENK----------------HYDSP 151

Query: 124 TCIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTD 183
           T   + T                     ++P          +  ++      +   +G D
Sbjct: 152 TNPAAPT---------------------RIPGGAC------SGAAVAVATNAVDFALGID 203

Query: 184 TGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           T G VRVPA YCG+ GF+ S+GA+S +G+IP++ S D+VG
Sbjct: 212 TVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVG 203

BLAST of Carg27606 vs. Swiss-Prot
Match: sp|Q9MUK5|TOC64_PEA (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.0e-30
Identity = 83/220 (37.73%), Postives = 114/220 (51.82%), Query Frame = 0

Query: 4   QDHGAFMEKFLLQPSSPSDQL----PLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPAS 63
           +D GAF+ K  L             PL+ L FA+ DIFD++G+V+ FG+PEW RTH PAS
Sbjct: 35  EDFGAFLLKLKLXXXXXXXXXXXPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  QTAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKP 123
            TA AV ++++ GATCIG T++DE+AY        G++           G++  + TP  
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAY--------GIS-----------GENKHFGTPTN 154

Query: 124 TCIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTD 183
             + +          R     + G  +    +        F +FSL           G D
Sbjct: 155 PAVPN----------RVPGGSSSGAAVAVAAN--------FVDFSL-----------GVD 206

Query: 184 TGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           T G VRVPA +CGI GFRPSHGAVS  G+IP++ S DTVG
Sbjct: 215 TSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVG 206

BLAST of Carg27606 vs. Swiss-Prot
Match: sp|F4KCL7|OE64M_ARATH (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.4e-29
Identity = 75/220 (34.09%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 4   QDHGAFMEKFLLQP----SSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPAS 63
           +D GAF+++F L P            L+GLTF++ D FDV  Y+TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPXXXXXXXXQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKP 123
           +TA  V ++LK GATC+GKTIMDE+ + ++                   G++  + TP  
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGII-------------------GENKHYGTPIN 164

Query: 124 TCIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTD 183
             +  +                       VP   +         ++    +++   +G D
Sbjct: 165 PLMPDN-----------------------VPGGCSSGS------AVSVGAELVDFSLGID 216

Query: 184 TGGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           T G VRVPA++CGI GFRPS G VS+ GV+P +QS +TVG
Sbjct: 225 TTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVG 216

BLAST of Carg27606 vs. TrEMBL
Match: tr|A0A0A0M1K1|A0A0A0M1K1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570110 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.6e-55
Identity = 122/219 (55.71%), Postives = 142/219 (64.84%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQ +GAFMEKFLLQPSSPSDQLPL GLTFAVKDIFD+DG+VTGFGNPEWLRTHPPA+ 
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAP V +IL+GGATCIG+TIMDEMAY +                    G++  + TP+  
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSIN-------------------GENFHYGTPQNP 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
           C  SD                      +VP   +         ++     ++   +GTDT
Sbjct: 121 C-ASD----------------------RVPGGSSSGS------AVAVGANLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGI GFRPSHGAVSTSGV+PMAQS DTVG
Sbjct: 181 GGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVG 171

BLAST of Carg27606 vs. TrEMBL
Match: tr|A0A1S3BPN7|A0A1S3BPN7_CUCME (amidase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.5e-54
Identity = 123/219 (56.16%), Postives = 143/219 (65.30%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQ +GAFMEKFLLQPSSPSDQLPL GLTFAVKDIFD+DG+VTGFGNPEWLRTHPPA+ 
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAP V +IL+GGATCIG+TIMDEMAY +                    G++  + TP+  
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSIN-------------------GENFHYGTPQNP 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
           C  SD                      +VP   +         ++     ++   +GTDT
Sbjct: 121 C-ASD----------------------RVPGGSSSGS------AVAVGANLVDFSLGTDT 170

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGI GFRPSHGAVSTSGV+PMAQSFDTVG
Sbjct: 181 GGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVG 170

BLAST of Carg27606 vs. TrEMBL
Match: tr|A0A1S4DYH0|A0A1S4DYH0_CUCME (amidase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.5e-54
Identity = 123/219 (56.16%), Postives = 143/219 (65.30%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAVQ +GAFMEKFLLQPSSPSDQLPL GLTFAVKDIFD+DG+VTGFGNPEWLRTHPPA+ 
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAP V +IL+GGATCIG+TIMDEMAY +                    G++  + TP+  
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSIN-------------------GENFHYGTPQNP 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
           C  SD                      +VP   +         ++     ++   +GTDT
Sbjct: 121 C-ASD----------------------RVPGGSSSGS------AVAVGANLVDFSLGTDT 170

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGI GFRPSHGAVSTSGV+PMAQSFDTVG
Sbjct: 181 GGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVG 170

BLAST of Carg27606 vs. TrEMBL
Match: tr|A0A1S3BQD5|A0A1S3BQD5_CUCME (amidase 1-like OS=Cucumis melo OX=3656 GN=LOC103492181 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.3e-54
Identity = 119/219 (54.34%), Postives = 142/219 (64.84%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAV D GAF+EKFLLQ SSPSDQLPL+GLTFAVKDIFD++G+V GFGNPEWLRTHPPA+Q
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAPAVL+IL+GGATCIGKT+MDEMA+ +                    G++  + TP+  
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSIN-------------------GENFHYGTPQNP 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
           C                   A G    ++P   +          +    K++   +GTDT
Sbjct: 121 C-------------------ASG----RIPGGSSSGS------GVAVAAKLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCG+ GFRPSHG VSTSGVIPM QSFDTVG
Sbjct: 181 GGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVG 171

BLAST of Carg27606 vs. TrEMBL
Match: tr|A0A0A0LZ24|A0A0A0LZ24_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570120 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 4.3e-54
Identity = 120/219 (54.79%), Postives = 142/219 (64.84%), Query Frame = 0

Query: 1   MAVQDHGAFMEKFLLQPSSPSDQLPLAGLTFAVKDIFDVDGYVTGFGNPEWLRTHPPASQ 60
           MAV D+GAF+EKFLLQ SSPSD+LPL+GLTFAVKDIFD++GYV GFGNPEWLRTHPPA+Q
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLSILKGGATCIGKTIMDEMAYRLLNIFISGVTSLIIIFFQYKWGKHSLWHTPKPT 120
           TAPAVL+IL+GGATCIGKTIMDEMA+ +                    G++  + TP+  
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSIN-------------------GENFHYGTPQ-N 120

Query: 121 CIRSDTWRIFQWLCRCCRCKACGFLLRQVPSSLTLSKLWFNEFSLEYVVKVIFSFVGTDT 180
              SD                      ++P   +          +    K++   +GTDT
Sbjct: 121 PHASD----------------------RIPGGSSSGS------GVAVAAKLVDFSLGTDT 171

Query: 181 GGSVRVPASYCGIFGFRPSHGAVSTSGVIPMAQSFDTVG 220
           GGSVRVPASYCGI GFRPSHG VSTSGVIPM QSFDTVG
Sbjct: 181 GGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVG 171

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022961591.11.4e-6162.10amidase 1-like [Cucurbita moschata][more]
XP_023516592.12.4e-6161.19amidase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022968725.11.6e-6061.19amidase 1 isoform X3 [Cucurbita maxima][more]
XP_022968726.15.6e-5859.36amidase 1 isoform X4 [Cucurbita maxima][more]
XP_022156705.18.1e-5755.25amidase 1 [Momordica charantia] >XP_022156710.1 amidase 1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G08980.17.3e-4445.83amidase 1[more]
AT3G17970.13.3e-3636.82translocon at the outer membrane of chloroplasts 64-III[more]
AT5G09420.13.5e-3034.09translocon at the outer membrane of chloroplasts 64-V[more]
AT3G25660.12.9e-0852.27Amidase family protein[more]
AT5G07360.15.1e-0540.91Amidase family protein[more]
Match NameE-valueIdentityDescription
sp|Q7XTK3|AMI1_ORYSJ5.9e-4345.58Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1[more]
sp|Q9FR37|AMI1_ARATH1.3e-4245.83Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1[more]
sp|Q9LVH5|OE64C_ARATH6.0e-3536.82Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP6... [more]
sp|Q9MUK5|TOC64_PEA2.0e-3037.73Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=... [more]
sp|F4KCL7|OE64M_ARATH6.4e-2934.09Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0M1K1|A0A0A0M1K1_CUCSA6.6e-5555.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570110 PE=4 SV=1[more]
tr|A0A1S3BPN7|A0A1S3BPN7_CUCME1.5e-5456.16amidase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=4 SV=1[more]
tr|A0A1S4DYH0|A0A1S4DYH0_CUCME1.5e-5456.16amidase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=4 SV=1[more]
tr|A0A1S3BQD5|A0A1S3BQD5_CUCME3.3e-5454.34amidase 1-like OS=Cucumis melo OX=3656 GN=LOC103492181 PE=4 SV=1[more]
tr|A0A0A0LZ24|A0A0A0LZ24_CUCSA4.3e-5454.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570120 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004040amidase activity
Vocabulary: INTERPRO
TermDefinition
IPR000120Amidase
IPR023631Amidase_dom
IPR036928AS_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006525 arginine metabolic process
biological_process GO:0018874 benzoate metabolic process
biological_process GO:0006558 L-phenylalanine metabolic process
biological_process GO:0006560 proline metabolic process
biological_process GO:0042207 styrene catabolic process
biological_process GO:0006568 tryptophan metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004040 amidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27606-RACarg27606-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3DG3DSA:3.90.1300.10coord: 171..220
e-value: 2.7E-20
score: 74.8
IPR036928Amidase signature (AS) superfamilyGENE3DG3DSA:3.90.1300.10coord: 8..100
e-value: 7.3E-15
score: 56.8
IPR036928Amidase signature (AS) superfamilySUPERFAMILYSSF75304Amidase signature (AS) enzymescoord: 176..219
IPR036928Amidase signature (AS) superfamilySUPERFAMILYSSF75304Amidase signature (AS) enzymescoord: 14..89
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 176..219
e-value: 1.2E-17
score: 63.9
coord: 20..86
e-value: 2.2E-6
score: 26.8
NoneNo IPR availablePANTHERPTHR11895:SF111AMIDASE 1coord: 176..219
NoneNo IPR availablePANTHERPTHR11895:SF111AMIDASE 1coord: 3..88
IPR000120AmidasePANTHERPTHR11895AMIDASEcoord: 176..219
IPR000120AmidasePANTHERPTHR11895AMIDASEcoord: 3..88

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg27606Cp4.1LG18g00700Cucurbita pepo (Zucchini)carcpeB1115
The following gene(s) are paralogous to this gene:

None