Carg27378 (gene) Silver-seed gourd

Overview
NameCarg27378
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPectinesterase inhibitor, putative
LocationCucurbita_argyrosperma_scaffold_396 : 85321 .. 85914 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCATATCAATTGCTGCCATGGCTATCACAATTCTATCAATCACCCACTTCCAGATACTCGCCGACAACCCCCAAAAGCCCTTCCTTACCAACGCCGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAACGACCTATGCCTATCAATCTTAGAGTCATCTCCCAACAGCAATGGCACAGATCTATATGGGCTAACAGAGATCATGTTGTATATTGCAGCTGAGAATGTGAGCCTCATACATGAACACCTAATGGAATTACAAAACAAGACAGAAATGGATACCTTCATGGATGAGTGTTTGACGGATTGCTTGGAGAGCTATCAGGACGCGACGGACCAAATCGAGGACTCGATCACAGCGCTCGAGTTCAAAGCGTATCATGATATCAAGACATGGGTAGCAGCAGCCATGAATGATGGAGCTGTGTGTGAAAATGGGTTCAAAGAGAAACCAGACCACAAGTCACCAATTGTGGAGATGTCTAAAGTTTTTGATCAGATCTGCAGCATTATTCTGGCTATGGCAAATATCTTGTCTCAGGGAAATAGGAATATGAACTCTGAGGATATATTTAATTTTTGA

mRNA sequence

ATGAAGCTCATATCAATTGCTGCCATGGCTATCACAATTCTATCAATCACCCACTTCCAGATACTCGCCGACAACCCCCAAAAGCCCTTCCTTACCAACGCCGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAACGACCTATGCCTATCAATCTTAGAGTCATCTCCCAACAGCAATGGCACAGATCTATATGGGCTAACAGAGATCATGTTGTATATTGCAGCTGAGAATGTGAGCCTCATACATGAACACCTAATGGAATTACAAAACAAGACAGAAATGGATACCTTCATGGATGAGTGTTTGACGGATTGCTTGGAGAGCTATCAGGACGCGACGGACCAAATCGAGGACTCGATCACAGCGCTCGAGTTCAAAGCGTATCATGATATCAAGACATGGGTAGCAGCAGCCATGAATGATGGAGCTGTGTGTGAAAATGGGTTCAAAGAGAAACCAGACCACAAGTCACCAATTGTGGAGATGTCTAAAGTTTTTGATCAGATCTGCAGCATTATTCTGGCTATGGCAAATATCTTGTCTCAGGGAAATAGGAATATGAACTCTGAGGATATATTTAATTTTTGA

Coding sequence (CDS)

ATGAAGCTCATATCAATTGCTGCCATGGCTATCACAATTCTATCAATCACCCACTTCCAGATACTCGCCGACAACCCCCAAAAGCCCTTCCTTACCAACGCCGATCTCATCACCAAAACCTGCAGCAGCACCCCTTACAACGACCTATGCCTATCAATCTTAGAGTCATCTCCCAACAGCAATGGCACAGATCTATATGGGCTAACAGAGATCATGTTGTATATTGCAGCTGAGAATGTGAGCCTCATACATGAACACCTAATGGAATTACAAAACAAGACAGAAATGGATACCTTCATGGATGAGTGTTTGACGGATTGCTTGGAGAGCTATCAGGACGCGACGGACCAAATCGAGGACTCGATCACAGCGCTCGAGTTCAAAGCGTATCATGATATCAAGACATGGGTAGCAGCAGCCATGAATGATGGAGCTGTGTGTGAAAATGGGTTCAAAGAGAAACCAGACCACAAGTCACCAATTGTGGAGATGTCTAAAGTTTTTGATCAGATCTGCAGCATTATTCTGGCTATGGCAAATATCTTGTCTCAGGGAAATAGGAATATGAACTCTGAGGATATATTTAATTTTTGA

Protein sequence

MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMANILSQGNRNMNSEDIFNF
Homology
[Close]
BLAST of Carg27378 vs. NCBI nr
Match: XP_022961546.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 390.6 bits (1002), Expect = 3.2e-105
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0 

Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]
BLAST of Carg27378 vs. NCBI nr
Match: XP_023515956.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388.7 bits (997), Expect = 1.2e-104
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0 

Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAITILSITHFQILAD+PQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITILSITHFQILADDPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]
BLAST of Carg27378 vs. NCBI nr
Match: XP_022968792.1 (pectinesterase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 384.4 bits (986), Expect = 2.3e-103
Identity = 192/197 (97.46%), Postives = 195/197 (98.98%), Query Frame = 0 

Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MKLISIAAMAIT+LS+THFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS
Sbjct: 1   MKLISIAAMAITVLSMTHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           NGTDLYGLTEIMLYIAAENVSLIHEHLMELQN TEMDTFMDECLTDCLE+YQDATDQIED
Sbjct: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNNTEMDTFMDECLTDCLENYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAM N
Sbjct: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMTN 180

Query: 181 ILSQGNRNMNSEDIFNF 198
           ILSQGNRNMNSEDIFNF
Sbjct: 181 ILSQGNRNMNSEDIFNF 197

[Close]
BLAST of Carg27378 vs. NCBI nr
Match: XP_011659828.1 (PREDICTED: putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein Csa_1G569235 [Cucumis sativus])

HSP 1 Score: 232.3 bits (591), Expect = 1.5e-57
Identity = 114/189 (60.32%), Postives = 154/189 (81.48%), Query Frame = 0 

Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60
           MK I+I A+A  ++S+  FQILA  PQ P  +   DLI+KTCSST Y+++C +IL+SSPN
Sbjct: 1   MKSIAI-AVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPN 60

Query: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIE 120
           S G DLYGL +I++ +AA+NVS I+E++ +LQN T +D+F+D CLTDCLES+QDA DQIE
Sbjct: 61  SKGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIE 120

Query: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMA 180
           DS+TALEFKAY+D+KTW++AAM+D A C++GFKEK  ++SPI +M+ VFDQICSIIL++ 
Sbjct: 121 DSVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSIN 180

Query: 181 NILSQGNRN 189
            +LSQGN N
Sbjct: 181 QLLSQGNTN 188

[Close]
BLAST of Carg27378 vs. NCBI nr
Match: XP_022970023.1 (pectinesterase inhibitor 7-like [Cucurbita maxima])

HSP 1 Score: 217.2 bits (552), Expect = 4.9e-53
Identity = 108/191 (56.54%), Postives = 145/191 (75.92%), Query Frame = 0 

Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 60
           MK IS+A   + ++S+  FQ   DN         +LITK CS T YNDLC +IL+++PNS
Sbjct: 1   MKPISVAVAVLVVVSLCSFQAQGDN---------ELITKACSRTAYNDLCKAILQAAPNS 60

Query: 61  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 120
           N  DLYGLT+  L IAA+NVS I++ + +LQN + +D+FMDECLTDCLE+YQDATDQIED
Sbjct: 61  NDADLYGLTQFALSIAAQNVSNIYQTIDKLQNDSSVDSFMDECLTDCLENYQDATDQIED 120

Query: 121 SITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMAN 180
           S+TALEFKA++D++TWVAAAM+D A C+ GFK+KP ++SP+ +MS VFDQICSI+L   +
Sbjct: 121 SVTALEFKAFNDVRTWVAAAMSDVATCDLGFKDKPGYQSPVHQMSAVFDQICSIVLTFNH 180

Query: 181 ILSQGNRNMNS 192
            L +GN ++ S
Sbjct: 181 CLIEGNVHLTS 182

[Close]
BLAST of Carg27378 vs. TAIR10
Match: AT3G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-12
Identity = 56/193 (29.02%), Postives = 95/193 (49.22%), Query Frame = 0 

Query: 3   LISIAAMAITILSITHFQILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNSNG 62
           L+ ++ +A+T  + TH       P  P +++A L    CS T Y + C + L SS N + 
Sbjct: 85  LLILSVVALTAGAFTH-----SPPHHPPISSASL-KSLCSVTRYPETCFNSLSSSLNESD 144

Query: 63  TDL--YGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 122
           + L    + E+ L +AA+ +S +      + +  E     D  + DC++ Y DA  Q+ D
Sbjct: 145 SKLNPESILELSLRVAAKEISNLSISFRSINDMPE-----DAAVGDCVKLYTDALSQLND 204

Query: 123 SITALEFK------------AYHDIKTWVAAAMNDGAVCENGFKEKPD-HKSPIVEMSKV 181
           SIT +E K               D+KTW++AAM DG  C +G +E      + I +  ++
Sbjct: 205 SITEIERKKKKGGNNWLTEEVVGDVKTWISAAMTDGETCSDGIEEMGTIVGNEIKKKMEM 264

[Close]
BLAST of Carg27378 vs. TAIR10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 69.7 bits (169), Expect = 2.3e-12
Identity = 51/172 (29.65%), Postives = 84/172 (48.84%), Query Frame = 0 

Query: 32  TNADLITKTCSST-------PYNDLCLSILESSPNS--NGTDLYGLTEIMLYIAAENVSL 91
           + +D+I +TC S         YN  C+S L +SP +  + T+L  L  + +  A +N + 
Sbjct: 21  STSDMIDQTCKSCAAKSTIFDYN-FCVSSLNNSPIALPSPTNLSSLALVPMLQALDNATA 80

Query: 92  IHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITA-LEFKAYHDIKTWVAAAM 151
               + +L    +  +F   CL DCLE Y+DATD++E+++   +  K    +   V+AAM
Sbjct: 81  TASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFITRKELGTVNVMVSAAM 140

Query: 152 NDGAVCENGFKEKPDH----------KSPIVEMSKVFDQICSIILAMANILS 184
                CENGF+E+ D            SPI + +    +   I L + N+LS
Sbjct: 141 ESAVTCENGFRERDDXXXXXXXVTTWTSPIGDENHKLFEFGQIALCIFNMLS 191

[Close]
BLAST of Carg27378 vs. TAIR10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 62.4 bits (150), Expect = 3.6e-10
Identity = 34/134 (25.37%), Postives = 67/134 (50.00%), Query Frame = 0 

Query: 50  CLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLE 109
           C++ L S P +       L  I L IA+ N S    ++     +  ++  +++ L DC +
Sbjct: 26  CVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNLEPALEDTLDDCSK 85

Query: 110 SYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFD 169
           +Y DA  Q++DS+ AL   ++ D+  W+  A++DG  CEN   ++  + + +   +    
Sbjct: 86  NYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTNLL 145

Query: 170 QICSIILAMANILS 184
           ++C   L +  IL+
Sbjct: 146 KLCKDALLINTILT 159

[Close]
BLAST of Carg27378 vs. TAIR10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-08
Identity = 48/185 (25.95%), Postives = 81/185 (43.78%), Query Frame = 0 

Query: 3   LISIAAMAITILSITHF--QILADNPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS 62
           ++++    + IL  + F  QIL  +P       +D I   C++T Y DLC S L S  NS
Sbjct: 1   MVTMMRPTLLILLFSTFLPQILTVDPPLLPSNGSDFIRLACNTTLYPDLCFSTLSSFANS 60

Query: 63  NGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIED 122
              D   L  + + +   N   +  +L    N+          L DC E+ +DA D +  
Sbjct: 61  IQNDSNRLARVAISLTLHNTLHLLSYLQNAYNRDH----PTPVLRDCFENLKDAVDGMRG 120

Query: 123 SITALE-----------FK-AYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVF 174
           S+  ++           F+    ++KTW++AA+ D   C +GFK+       + E   + 
Sbjct: 121 SMKQMKELVSASGSIESFRFQMSNVKTWLSAALTDEYTCTDGFKD-------VHEDDSIK 174

[Close]
BLAST of Carg27378 vs. TAIR10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.0 bits (136), Expect = 1.5e-08
Identity = 46/174 (26.44%), Postives = 84/174 (48.28%), Query Frame = 0 

Query: 13  ILSITHFQILAD---NPQKPFLTNADLITKTCSSTPYNDLCLSILESSPNS-NGTDLYGL 72
           +L +  F +L++   N Q     +    T T     YN LC++ LE++P +    DL GL
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYN-LCVTSLETNPQAKTAKDLAGL 63

Query: 73  TEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFK 132
                  A    + +   + ++    +++      L DCL+ Y DA   + +++  ++ +
Sbjct: 64  VMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSR 123

Query: 133 AYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMANIL 183
            Y  +KT ++AAM+  + CE GFKE+    SP+ + +    Q+  I LA  N+L
Sbjct: 124 NYPTVKTVLSAAMDTPSTCETGFKER-KAPSPVTKENDNLYQMILIPLAFTNML 175

[Close]
BLAST of Carg27378 vs. Swiss-Prot
Match: sp|Q8GT41|PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 46/156 (29.49%), Postives = 81/156 (51.92%), Query Frame = 0 

Query: 33  NADLITKTCSSTPYN------DLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEH 92
           +AD++  TC            D C+  L + P S+  DL GL  I   +A ++ S I   
Sbjct: 23  SADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTF 82

Query: 93  LMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAV 152
           +  +  K+++D  + + L DC+  Y DA   ++++I   + K Y      ++AA++D   
Sbjct: 83  IGRIL-KSKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVT 142

Query: 153 CENGFKEKPDHKSPIVEMSKVFDQICSIILAMANIL 183
           CE+GFKEK    SP+ + +K + Q+ +I LA+  +L
Sbjct: 143 CEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]
BLAST of Carg27378 vs. Swiss-Prot
Match: sp|O49298|PME6_ARATH (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 4.0e-06
Identity = 34/125 (27.20%), Postives = 62/125 (49.60%), Query Frame = 0 

Query: 33  NADLITKTCSSTPYNDLCLSILESSP---NSNGTDLYGLTEIMLYIAAENVSLIHEHLME 92
           NA  IT +C  TPY  +C   + +SP     + TD +   ++++    +    +H  +  
Sbjct: 31  NAHFIT-SCKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSS 90

Query: 93  LQNKTEMDTFMDECLTDCLESYQDATDQIEDSITAL-EFKAYHDIKTWVAAAMNDGAVCE 152
           L+    +       L DCLE Y+D  DQ+  S  +  ++ + HD +T ++AA+ +   C 
Sbjct: 91  LKQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCR 150

Query: 153 NGFKE 154
           NGF++
Sbjct: 151 NGFRD 154

[Close]
BLAST of Carg27378 vs. TrEMBL
Match: tr|A0A0A0M1H8|A0A0A0M1H8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 9.6e-58
Identity = 114/189 (60.32%), Postives = 154/189 (81.48%), Query Frame = 0 

Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60
           MK I+I A+A  ++S+  FQILA  PQ P  +   DLI+KTCSST Y+++C +IL+SSPN
Sbjct: 1   MKSIAI-AVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPN 60

Query: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIE 120
           S G DLYGL +I++ +AA+NVS I+E++ +LQN T +D+F+D CLTDCLES+QDA DQIE
Sbjct: 61  SKGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIE 120

Query: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKEKPDHKSPIVEMSKVFDQICSIILAMA 180
           DS+TALEFKAY+D+KTW++AAM+D A C++GFKEK  ++SPI +M+ VFDQICSIIL++ 
Sbjct: 121 DSVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSIN 180

Query: 181 NILSQGNRN 189
            +LSQGN N
Sbjct: 181 QLLSQGNTN 188

[Close]
BLAST of Carg27378 vs. TrEMBL
Match: tr|A0A1S3BQL1|A0A1S3BQL1_CUCME (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492143 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 9.4e-45
Identity = 96/154 (62.34%), Postives = 122/154 (79.22%), Query Frame = 0 

Query: 1   MKLISIAAMAITILSITHFQILADNPQKPFLTNA-DLITKTCSSTPYNDLCLSILESSPN 60
           MK I+I A+A  ++S+  FQILA  PQ P  +   DLITKTCSSTP+ ++C +IL+SSPN
Sbjct: 1   MKSIAI-AVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPN 60

Query: 61  SNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNKTEMDTFMDECLTDCLESYQDATDQIE 120
           S G DLYGL +I++ IAA NVS I+E + +LQN T +D+F+D CLTDCLESYQDA DQIE
Sbjct: 61  SKGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIE 120

Query: 121 DSITALEFKAYHDIKTWVAAAMNDGAVCENGFKE 154
           DS+TALEFKAY+D+ TWV+AAM+D A C+ GFKE
Sbjct: 121 DSVTALEFKAYNDVNTWVSAAMSDAATCDMGFKE 153

[Close]
BLAST of Carg27378 vs. TrEMBL
Match: tr|A0A200PWM0|A0A200PWM0_9MAGN (Pectinesterase inhibitor domain OS=Macleaya cordata OX=56857 GN=BVC80_9091g128 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.8e-30
Identity = 68/152 (44.74%), Postives = 95/152 (62.50%), Query Frame = 0 

Query: 32  TNADLITKTCSSTPYNDLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEHLMELQ 91
           T  DLI KTCS   Y +LC+S L   P+S   DL GL  I + IA  N S I  H+ +L 
Sbjct: 37  TGDDLIVKTCSHAVYKELCVSTLRDDPDSQEADLAGLASIAIKIAMTNGSEIFNHIGQLL 96

Query: 92  NKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAVCENGF 151
                  F  +CL+DC E+Y DA DQ+EDS+ A++ K ++D+ TWV AAM D   CE+GF
Sbjct: 97  KAANYAPFTQQCLSDCSENYMDAVDQLEDSLAAIDAKGFNDVSTWVQAAMTDAESCEDGF 156

Query: 152 KEKPDHKSPIVEMSKVFDQICSIILAMANILS 184
           KE+  H+S + + + VF+Q+CS  LA+ N+L+
Sbjct: 157 KEEVGHQSLLTDRNAVFNQLCSNALALTNLLA 188

[Close]
BLAST of Carg27378 vs. TrEMBL
Match: tr|B9RFD8|B9RFD8_RICCO (Pectinesterase inhibitor, putative OS=Ricinus communis OX=3988 GN=RCOM_1433910 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-29
Identity = 68/151 (45.03%), Postives = 103/151 (68.21%), Query Frame = 0 

Query: 34  ADLITKTCSSTPYNDLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHEHLMELQNK 93
           +DLI+KTC  T Y D C ++L ++P S+  DL  LT+  L +A+ N   IH+ + ++ +K
Sbjct: 22  SDLISKTCDQTLYKDYCKTVLGAAPESDVKDLPSLTKYALKMASLNGVKIHKKIDQI-SK 81

Query: 94  TEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGAVCENGFKE 153
           +  D F+ +CL DC E YQDA DQ+EDS  A++ K+Y+D+ TWV AAM D   CE+ FKE
Sbjct: 82  SNKDEFIQQCLDDCSEIYQDAIDQVEDSTAAVDGKSYNDVNTWVTAAMTDSQTCEDAFKE 141

Query: 154 KPDHKSPIVEMSKVFDQICSIILAMANILSQ 185
           +   KSP+ + +  F+Q+CSIIL M+N+L++
Sbjct: 142 QDGVKSPLTDDNTKFNQLCSIILTMSNLLAK 171

[Close]
BLAST of Carg27378 vs. TrEMBL
Match: tr|A0A2H5PQ01|A0A2H5PQ01_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_156750 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.2e-28
Identity = 70/158 (44.30%), Postives = 103/158 (65.19%), Query Frame = 0 

Query: 26  PQKPFLTNADLITKTCSSTPYNDLCLSILESSPNSNGTDLYGLTEIMLYIAAENVSLIHE 85
           P K    + DLIT TC+ T Y +LC++ L S  +S G+DL GL +I L I   N + I  
Sbjct: 19  PCKNTEASNDLITTTCAKTMYKELCIASLRSDKDSRGSDLQGLGKISLKITLANANEIQG 78

Query: 86  HLMELQNKTEMDTFMDECLTDCLESYQDATDQIEDSITALEFKAYHDIKTWVAAAMNDGA 145
           ++ EL  K +   F+ +CL DC E+ QDA  +I+DSI A++ ++Y D+ TWV+AAM+   
Sbjct: 79  YISELLKK-DGKPFLQQCLKDCSENLQDAILEIKDSIAAIDSRSYGDVNTWVSAAMSYEE 138

Query: 146 VCENGFKEKPDHKSPIVEMSKVFDQICSIILAMANILS 184
            CE+GFKEKP  KSP+ +M+  F Q+CSI L ++N+L+
Sbjct: 139 SCEDGFKEKPGFKSPLTQMNAKFSQLCSISLTISNLLA 175

The following BLAST results are available for this feature:
BLAST of Carg27378 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022961546.13.2e-105100.00pectinesterase inhibitor-like [Cucurbita moschata][more]
XP_023515956.11.2e-10499.49pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo][more]
XP_022968792.12.3e-10397.46pectinesterase inhibitor-like [Cucurbita maxima][more]
XP_011659828.11.5e-5760.32PREDICTED: putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetic... [more]
XP_022970023.14.9e-5356.54pectinesterase inhibitor 7-like [Cucurbita maxima][more]
BLAST of Carg27378 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G47670.11.3e-1229.02Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G38610.12.3e-1229.65Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G02250.13.6e-1025.37Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G70720.11.2e-0825.95Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G46940.11.5e-0826.44Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
BLAST of Carg27378 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8GT41|PLA1_PLAAC3.3e-1329.49Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1[more]
sp|O49298|PME6_ARATH4.0e-0627.20Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=37... [more]
BLAST of Carg27378 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0M1H8|A0A0A0M1H8_CUCSA9.6e-5860.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 SV=1[more]
tr|A0A1S3BQL1|A0A1S3BQL1_CUCME9.4e-4562.34putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492143 PE=4 SV=1[more]
tr|A0A200PWM0|A0A200PWM0_9MAGN3.8e-3044.74Pectinesterase inhibitor domain OS=Macleaya cordata OX=56857 GN=BVC80_9091g128 P... [more]
tr|B9RFD8|B9RFD8_RICCO1.1e-2945.03Pectinesterase inhibitor, putative OS=Ricinus communis OX=3988 GN=RCOM_1433910 P... [more]
tr|A0A2H5PQ01|A0A2H5PQ01_CITUN1.2e-2844.30Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_156750 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR035513Invertase/methylesterase_inhib
IPR006501Pectinesterase_inhib_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27378-RACarg27378-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 32..178
e-value: 1.7E-33
score: 127.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 35..178
e-value: 1.2E-31
score: 110.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 5..182
e-value: 2.6E-38
score: 129.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3DG3DSA:1.20.140.40coord: 33..184
e-value: 1.4E-42
score: 147.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILYSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 35..183
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 9..183
NoneNo IPR availableCDDcd15801PMEI-like_1coord: 36..182
e-value: 1.17525E-52
score: 167.123

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg27378Cucurbita moschata (Rifu)carcmoB0162
Carg27378Cucurbita maxima (Rimu)carcmaB0153
Carg27378Watermelon (97103) v1carwmB0116
Carg27378Watermelon (97103) v2carwmbB0122