Carg27288 (gene) Silver-seed gourd

NameCarg27288
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionEndoglucanase
LocationCucurbita_argyrosperma_scaffold_226 : 149654 .. 151650 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGAACCCTCTCATCTCCTTTCTTCTCATCCTTCCTTTCACTCATTCATTATCCCATGACTATCACCACGCGCTCTCCATCACTCTCCTCTACTTCGAATCCCAACTCTCCGGCCGCCTCCCCTACAACCAAAGACTCCCTTGGCGCCACCACTCTGCCCTCTCCGATGGCCTCCACCAAGGAGTACACACACCTTTTTCTCTTTCTTTCTTTAACTTTATCCAATAATATTCATTTGCTGTTTTGTTTTGATTTTCAGGTGGACTTAGTGGGAGGCTACTACGACGCCGGCGACAACATCAAGTTCGGTTTACCAATGGCCTTCACCGTCACAATGCTGTCATGGGGTGTCATTGAATATGGAGAACAACTCGCGGCTGCCGGAGAGTACATTCACGCGCTGGAAGCTATCAAGTGGGGCACTGATTACTTCATCAAAGCCCATCCCCACCCCAACGTCTTCTGGGCACAGGTTACTTGCTCAACCAAATTCAGGCGGCGCGTGAATTACACGCGCATCATATTAATGATTGTAACAAATCAAGTTTCAATCTTTCCAGGTGGGAGATGGGGCAACGGATCACTACTGCTGGCAGCGGCCGGAGGACATGACGACGTCACGGCAAGCCTACAAGATTGACGAAAACAACCCGGGTTCCGATGTGGCTGGAGAGACGGCGGCGGCCATGGCGGCAGCAGCAATTGTGTTCAGTAGAACAAACCCACACTACTCCCATGTACTGCTGCACCATGCACAAGAGGCAAGTAGAACAACTCAAAAGAAAATAACATGAAATTAATAAATAAAATAAATGGAACTGTTGAACAAAAAGAATGAACTAAACTTTATTTCATAATTTTTAATTTCATAATAAATTAATTAGAAGTAGTGATGAAGTTGCTATAAATATAAATTGCCTGTAGTTGGAAAATTTTATTTATCTATACAATTTGTTCTTAAAATATATTTTTTTTTCTAAAATATTTTTATTCTTAAAATAATAACTTGAACTAGTGATGATATTTTAACAAATATTGTGGTGCAGTTGTTTGAGTTTGGCAATAAGCACAGAGGGAAATACGACGAGAGTATTAAAGAGGCAAAGGGGTATTACTCGTCAGCGAGCGGGTACAAGGACGAGCTGCTGTGGGCAGCAATGTGGCTGTACAAGAGCAGTGGAAGGGAAGAGTACCTGAAATATGTGCTGGAAAATGGGAAGAATTTTGGTGGGATTACTTGGGGCATCAATGAGTTTAGTTGGGATGTTAAGTATGCTGGCCTCCAAATCCTTGTATCCATGGTAATTTCTCTCTAGAACTTGATTTTGTTGGGTAAAAAGTAATAAAAATGGTAACTTTTTTTCGTTTAATTTTAGTTGCGAGTGGAAGAACAACCGAAAGAACAGAAGTCCATAATAGAAGCCTACCGAGCAAAGGCGGAGCATTACCTTTGTGGTTGTCTCAACAAGAACAAGAACAACTTGACTAACCTGAAACGCACTCCAGGGGGCTTACTCTACACTCGTAAATGGAATAACCTCCAATACGTTTCCACCGCAGCTTTTCTTCTCACCCTCTACTCCGACCATCTCCAAGCCTCAAACCAACGTCTCACATGTCATCAACACAAAGTGGGCCCCCAAGAGATGTTGACTTTTGCGAAATCCCAAGTTGACTACATCTTGGGGGACAATCCAATGGCCATGAGCTACTTGGTCGGGTTTGGTCCACGGTATCCTCAAAGGGTGCACCACAGAGGAGCGTCGATTGAATCGATCAAAACGAACGGGGGATTTATCGGGTGCACGCAGGGGTATGATAATTGGTATGCTCGCCTTGATCCGAACCCTAATGTGTTGATGGGTGGTGTGGTGGGTGGACCCAATGAGAAAGATGAGTTCCATGATGAACGATCTAATTATATGCAAACTGAAGCCTGTACTTACAATACGGCGCCGTTGGTTGGTGTGTTTGCCAAATTGAGCCAAAATTAA

mRNA sequence

ATGTTGAACCCTCTCATCTCCTTTCTTCTCATCCTTCCTTTCACTCATTCATTATCCCATGACTATCACCACGCGCTCTCCATCACTCTCCTCTACTTCGAATCCCAACTCTCCGGCCGCCTCCCCTACAACCAAAGACTCCCTTGGCGCCACCACTCTGCCCTCTCCGATGGCCTCCACCAAGGAGTGGACTTAGTGGGAGGCTACTACGACGCCGGCGACAACATCAAGTTCGGTTTACCAATGGCCTTCACCGTCACAATGCTGTCATGGGGTGTCATTGAATATGGAGAACAACTCGCGGCTGCCGGAGAGTACATTCACGCGCTGGAAGCTATCAAGTGGGGCACTGATTACTTCATCAAAGCCCATCCCCACCCCAACGTCTTCTGGGCACAGGTGGGAGATGGGGCAACGGATCACTACTGCTGGCAGCGGCCGGAGGACATGACGACGTCACGGCAAGCCTACAAGATTGACGAAAACAACCCGGGTTCCGATGTGGCTGGAGAGACGGCGGCGGCCATGGCGGCAGCAGCAATTGTGTTCAGTAGAACAAACCCACACTACTCCCATGTACTGCTGCACCATGCACAAGAGTTGTTTGAGTTTGGCAATAAGCACAGAGGGAAATACGACGAGAGTATTAAAGAGGCAAAGGGGTATTACTCGTCAGCGAGCGGGTACAAGGACGAGCTGCTGTGGGCAGCAATGTGGCTGTACAAGAGCAGTGGAAGGGAAGAGTACCTGAAATATGTGCTGGAAAATGGGAAGAATTTTGGTGGGATTACTTGGGGCATCAATGAGTTTAGTTGGGATGTTAAGTATGCTGGCCTCCAAATCCTTGTATCCATGTTGCGAGTGGAAGAACAACCGAAAGAACAGAAGTCCATAATAGAAGCCTACCGAGCAAAGGCGGAGCATTACCTTTGTGGTTGTCTCAACAAGAACAAGAACAACTTGACTAACCTGAAACGCACTCCAGGGGGCTTACTCTACACTCGTAAATGGAATAACCTCCAATACGTTTCCACCGCAGCTTTTCTTCTCACCCTCTACTCCGACCATCTCCAAGCCTCAAACCAACGTCTCACATGTCATCAACACAAAGTGGGCCCCCAAGAGATGTTGACTTTTGCGAAATCCCAAGTTGACTACATCTTGGGGGACAATCCAATGGCCATGAGCTACTTGGTCGGGTTTGGTCCACGGTATCCTCAAAGGGTGCACCACAGAGGAGCGTCGATTGAATCGATCAAAACGAACGGGGGATTTATCGGGTGCACGCAGGGGTATGATAATTGGTATGCTCGCCTTGATCCGAACCCTAATGTGTTGATGGGTGGTGTGGTGGGTGGACCCAATGAGAAAGATGAGTTCCATGATGAACGATCTAATTATATGCAAACTGAAGCCTGTACTTACAATACGGCGCCGTTGGTTGGTGTGTTTGCCAAATTGAGCCAAAATTAA

Coding sequence (CDS)

ATGTTGAACCCTCTCATCTCCTTTCTTCTCATCCTTCCTTTCACTCATTCATTATCCCATGACTATCACCACGCGCTCTCCATCACTCTCCTCTACTTCGAATCCCAACTCTCCGGCCGCCTCCCCTACAACCAAAGACTCCCTTGGCGCCACCACTCTGCCCTCTCCGATGGCCTCCACCAAGGAGTGGACTTAGTGGGAGGCTACTACGACGCCGGCGACAACATCAAGTTCGGTTTACCAATGGCCTTCACCGTCACAATGCTGTCATGGGGTGTCATTGAATATGGAGAACAACTCGCGGCTGCCGGAGAGTACATTCACGCGCTGGAAGCTATCAAGTGGGGCACTGATTACTTCATCAAAGCCCATCCCCACCCCAACGTCTTCTGGGCACAGGTGGGAGATGGGGCAACGGATCACTACTGCTGGCAGCGGCCGGAGGACATGACGACGTCACGGCAAGCCTACAAGATTGACGAAAACAACCCGGGTTCCGATGTGGCTGGAGAGACGGCGGCGGCCATGGCGGCAGCAGCAATTGTGTTCAGTAGAACAAACCCACACTACTCCCATGTACTGCTGCACCATGCACAAGAGTTGTTTGAGTTTGGCAATAAGCACAGAGGGAAATACGACGAGAGTATTAAAGAGGCAAAGGGGTATTACTCGTCAGCGAGCGGGTACAAGGACGAGCTGCTGTGGGCAGCAATGTGGCTGTACAAGAGCAGTGGAAGGGAAGAGTACCTGAAATATGTGCTGGAAAATGGGAAGAATTTTGGTGGGATTACTTGGGGCATCAATGAGTTTAGTTGGGATGTTAAGTATGCTGGCCTCCAAATCCTTGTATCCATGTTGCGAGTGGAAGAACAACCGAAAGAACAGAAGTCCATAATAGAAGCCTACCGAGCAAAGGCGGAGCATTACCTTTGTGGTTGTCTCAACAAGAACAAGAACAACTTGACTAACCTGAAACGCACTCCAGGGGGCTTACTCTACACTCGTAAATGGAATAACCTCCAATACGTTTCCACCGCAGCTTTTCTTCTCACCCTCTACTCCGACCATCTCCAAGCCTCAAACCAACGTCTCACATGTCATCAACACAAAGTGGGCCCCCAAGAGATGTTGACTTTTGCGAAATCCCAAGTTGACTACATCTTGGGGGACAATCCAATGGCCATGAGCTACTTGGTCGGGTTTGGTCCACGGTATCCTCAAAGGGTGCACCACAGAGGAGCGTCGATTGAATCGATCAAAACGAACGGGGGATTTATCGGGTGCACGCAGGGGTATGATAATTGGTATGCTCGCCTTGATCCGAACCCTAATGTGTTGATGGGTGGTGTGGTGGGTGGACCCAATGAGAAAGATGAGTTCCATGATGAACGATCTAATTATATGCAAACTGAAGCCTGTACTTACAATACGGCGCCGTTGGTTGGTGTGTTTGCCAAATTGAGCCAAAATTAA

Protein sequence

MLNPLISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRAKAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLTCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFAKLSQN
BLAST of Carg27288 vs. NCBI nr
Match: XP_022932240.1 (endoglucanase 11-like [Cucurbita moschata])

HSP 1 Score: 1010.0 bits (2610), Expect = 2.8e-291
Identity = 480/490 (97.96%), Postives = 485/490 (98.98%), Query Frame = 0

Query: 1   MLNPLISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLH 60
           MLNP ISFLLILPFTHSLSHDYHHALSITLLYFESQ SGRLPYNQRLPWRHHSALSDGLH
Sbjct: 1   MLNPFISFLLILPFTHSLSHDYHHALSITLLYFESQRSGRLPYNQRLPWRHHSALSDGLH 60

Query: 61  QGVDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYF 120
           QGVDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLA AGEY HALEAIKWGTDYF
Sbjct: 61  QGVDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLATAGEYTHALEAIKWGTDYF 120

Query: 121 IKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAA 180
           IKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAA
Sbjct: 121 IKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAA 180

Query: 181 IVFSRTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWL 240
           IVFSRTNPHYSHVLLHHAQELFEFG+K+RGKYDESIKEAKGYYSS SGYKDELLWAAMWL
Sbjct: 181 IVFSRTNPHYSHVLLHHAQELFEFGDKNRGKYDESIKEAKGYYSSVSGYKDELLWAAMWL 240

Query: 241 YKSSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIE 300
           YKSSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIE
Sbjct: 241 YKSSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIE 300

Query: 301 AYRAKAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQAS 360
           AYRAKAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLT+YSDHLQAS
Sbjct: 301 AYRAKAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTVYSDHLQAS 360

Query: 361 NQRLTCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIK 420
           NQRLTCHQH+VGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIK
Sbjct: 361 NQRLTCHQHEVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIK 420

Query: 421 TNGGFIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPL 480
           TNGGFIGCTQGYD+WYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPL
Sbjct: 421 TNGGFIGCTQGYDSWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPL 480

Query: 481 VGVFAKLSQN 491
           VGVFAKLSQN
Sbjct: 481 VGVFAKLSQN 490

BLAST of Carg27288 vs. NCBI nr
Match: XP_023007593.1 (endoglucanase 11-like [Cucurbita maxima])

HSP 1 Score: 978.0 bits (2527), Expect = 1.2e-281
Identity = 467/490 (95.31%), Postives = 476/490 (97.14%), Query Frame = 0

Query: 1   MLNPLISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLH 60
           MLNP ISFLLILPFTHSLSHDYHHALSITLLYFESQ SGRLPYNQRLPWRHHSALSDGLH
Sbjct: 1   MLNPFISFLLILPFTHSLSHDYHHALSITLLYFESQRSGRLPYNQRLPWRHHSALSDGLH 60

Query: 61  QGVDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYF 120
           QGVDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEY HALEAIKWGTDYF
Sbjct: 61  QGVDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYTHALEAIKWGTDYF 120

Query: 121 IKAHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAA 180
           IKAHPHPNVFWAQVGDG TDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAA
Sbjct: 121 IKAHPHPNVFWAQVGDGVTDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAA 180

Query: 181 IVFSRTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWL 240
           IVFSRTNPHYSHVLLHHAQELFEFG+KHRGKYDESI+EAKGYY S SGYKDELLWAAMWL
Sbjct: 181 IVFSRTNPHYSHVLLHHAQELFEFGDKHRGKYDESIEEAKGYYPSVSGYKDELLWAAMWL 240

Query: 241 YKSSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIE 300
           YKSSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSML VEEQ KE KSI+E
Sbjct: 241 YKSSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLSVEEQSKEHKSIME 300

Query: 301 AYRAKAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQAS 360
           AYRAKAE+YLCGCL  NK+N TNLKRTP GLLYTRKWNNLQ+VSTAAFLLT+YSDHLQAS
Sbjct: 301 AYRAKAEYYLCGCL--NKSNSTNLKRTPEGLLYTRKWNNLQFVSTAAFLLTVYSDHLQAS 360

Query: 361 NQRLTCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIK 420
           NQRLTCHQH+VGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIK
Sbjct: 361 NQRLTCHQHEVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIK 420

Query: 421 TNGGFIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPL 480
           TNGGFIGCTQGYDNWYARLDPNPNVLMG VVGGPNEKDEFHDERSNYMQTEACTYNTAPL
Sbjct: 421 TNGGFIGCTQGYDNWYARLDPNPNVLMGAVVGGPNEKDEFHDERSNYMQTEACTYNTAPL 480

Query: 481 VGVFAKLSQN 491
           VGVFAKLSQ+
Sbjct: 481 VGVFAKLSQS 488

BLAST of Carg27288 vs. NCBI nr
Match: XP_004149358.2 (PREDICTED: endoglucanase 11-like [Cucumis sativus])

HSP 1 Score: 847.8 bits (2189), Expect = 1.8e-242
Identity = 400/486 (82.30%), Postives = 441/486 (90.74%), Query Frame = 0

Query: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64
           +I FLLILP THS   +Y  ALSI+LLYFESQ SGRLPYNQRL WRHHS L+DGLHQGVD
Sbjct: 1   MIFFLLILPLTHSF--NYQQALSISLLYFESQRSGRLPYNQRLTWRHHSGLTDGLHQGVD 60

Query: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124
           LVGGYYDAGDN+KFGLPMAFTVTMLSWGVIEYGEQ+AAAGEYIHALEAIKWGTDYFIKAH
Sbjct: 61  LVGGYYDAGDNVKFGLPMAFTVTMLSWGVIEYGEQIAAAGEYIHALEAIKWGTDYFIKAH 120

Query: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184
           PHPNV WAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS
Sbjct: 121 PHPNVLWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 180

Query: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244
           RTNPHYSH+LLHHA+ELFEFG+K+RGKYDESI+  KGYY SASGY DELLWAA WLYK+S
Sbjct: 181 RTNPHYSHLLLHHAEELFEFGDKYRGKYDESIEVVKGYYPSASGYMDELLWAATWLYKAS 240

Query: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304
           G+EEYLKYV+EN  +FGGI+W INEFSWD+KYAGLQIL SML VEE+ KE+K I+EAY++
Sbjct: 241 GKEEYLKYVIENALDFGGISWAINEFSWDIKYAGLQILASMLAVEEKQKEEKLIVEAYKS 300

Query: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364
           KAEHYLC CLNK+  N  NLKRTPGGLLYTRKWNNLQYV+TA FLLTLYSDHL++SNQRL
Sbjct: 301 KAEHYLCSCLNKSSTN-NNLKRTPGGLLYTRKWNNLQYVTTATFLLTLYSDHLESSNQRL 360

Query: 365 TC-HQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNG 424
            C +  +VGP+EML+ AKSQVDYILG+NPM MSYLVGFG RYP+RVHHRGAS+ES+K   
Sbjct: 361 RCSNDDEVGPEEMLSLAKSQVDYILGENPMGMSYLVGFGGRYPERVHHRGASMESVKEKR 420

Query: 425 GFIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGV 484
           GFIGC QGYD+WY R+D NPNVLMG VVGGPNE+DEF+DERSN+ QTEACTYNTAPLVGV
Sbjct: 421 GFIGCVQGYDDWYGRVDGNPNVLMGAVVGGPNERDEFNDERSNFRQTEACTYNTAPLVGV 480

Query: 485 FAKLSQ 490
           FA+ +Q
Sbjct: 481 FARFNQ 483

BLAST of Carg27288 vs. NCBI nr
Match: XP_018812066.1 (PREDICTED: endoglucanase 11-like isoform X2 [Juglans regia])

HSP 1 Score: 782.3 bits (2019), Expect = 9.4e-223
Identity = 361/483 (74.74%), Postives = 417/483 (86.34%), Query Frame = 0

Query: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64
           L+S   + P +  LS DY  ALS +LLYFESQ SGRLP+NQR+ WRHHS L+DGL QGVD
Sbjct: 23  LLSIFSLFP-SSCLSFDYGEALSKSLLYFESQRSGRLPHNQRVSWRHHSGLTDGLEQGVD 82

Query: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124
           LVGGYYDAGDN+KFGLPMAFTVTMLSW V+EYGEQ+A AGEY+HALEAIKWGTDYFIKAH
Sbjct: 83  LVGGYYDAGDNVKFGLPMAFTVTMLSWSVLEYGEQIAGAGEYLHALEAIKWGTDYFIKAH 142

Query: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184
            HPNV WAQVG+G TDHYCWQRPEDMTTSRQAYK+D NNPGSD+AGETAAAMAAA++VF 
Sbjct: 143 THPNVLWAQVGNGDTDHYCWQRPEDMTTSRQAYKVDANNPGSDIAGETAAAMAAASMVFR 202

Query: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244
           +TNPHYSH+L+HHAQ+LFEFG+K+RGKYDESIK +KGYYSS SGY DELLWAA+WLYK++
Sbjct: 203 KTNPHYSHLLIHHAQQLFEFGDKYRGKYDESIKVSKGYYSSVSGYMDELLWAALWLYKAT 262

Query: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304
            +EEYL YVLEN  +FGGITW + EFSWDVKY G+QI+ SML +E++ ++ K ++E YR+
Sbjct: 263 DKEEYLNYVLENAHSFGGITWAMTEFSWDVKYVGVQIIASMLLMEKKHEKHKHVLEQYRS 322

Query: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364
           KAEH+LC CL  N NN TN++RTPGGLLYTR+WNN+QYVSTAAFLLTLYSD+L+ SNQ L
Sbjct: 323 KAEHFLCACL--NMNNATNVQRTPGGLLYTRQWNNMQYVSTAAFLLTLYSDYLRTSNQEL 382

Query: 365 TCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGG 424
            CH   +GPQE+ +FAKSQVDYILG NP A+SYLVG+G  YP RVHHRGASIES K N G
Sbjct: 383 NCHNGVLGPQEIFSFAKSQVDYILGSNPKALSYLVGYGSNYPYRVHHRGASIESYKRNQG 442

Query: 425 FIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVF 484
           FIGCTQGYDNWY R DPNPN L+G +VGGP+ KD F DERSNY+QTEACTYN AP+VGVF
Sbjct: 443 FIGCTQGYDNWYGRHDPNPNTLVGALVGGPDNKDRFKDERSNYIQTEACTYNVAPVVGVF 502

Query: 485 AKL 488
           AKL
Sbjct: 503 AKL 502

BLAST of Carg27288 vs. NCBI nr
Match: OMP04364.1 (Glycoside hydrolase, family 9 [Corchorus olitorius])

HSP 1 Score: 780.0 bits (2013), Expect = 4.7e-222
Identity = 361/482 (74.90%), Postives = 417/482 (86.51%), Query Frame = 0

Query: 8   FLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVG 67
           FL ILP T     DY  ALS +LLYFESQ SGRLPYNQR+ WRHHS L+DGL QGVDLVG
Sbjct: 35  FLAILPLTQPF--DYGKALSKSLLYFESQRSGRLPYNQRVTWRHHSGLTDGLEQGVDLVG 94

Query: 68  GYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHP 127
           GYYDAGDN+KFGLPMAFT+TMLSWGVI+YG+++A AGEY HALEAIKWGTDYFIKAH HP
Sbjct: 95  GYYDAGDNVKFGLPMAFTITMLSWGVIDYGDEIAGAGEYSHALEAIKWGTDYFIKAHTHP 154

Query: 128 NVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTN 187
           NV WA+VGDG TDHYCWQRPEDMTTSRQAYK+DE NPGSDVAGETAAAMA+A+IVF +TN
Sbjct: 155 NVLWAEVGDGDTDHYCWQRPEDMTTSRQAYKVDEKNPGSDVAGETAAAMASASIVFRKTN 214

Query: 188 PHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGRE 247
           PHYSH+LL HA++LFEFG+K+RGKYDES+K  KGYYSS SG+ DELLWAA+WLYK++ +E
Sbjct: 215 PHYSHLLLQHAKQLFEFGDKYRGKYDESVKVVKGYYSSVSGFTDELLWAALWLYKATDKE 274

Query: 248 EYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPK--EQKSIIEAYRAK 307
           +YL+Y LE    FGGITW INEFSWDVK+AGLQI+ SML +EE+ +  E K+++E YR+K
Sbjct: 275 DYLRYALEKAYEFGGITWAINEFSWDVKFAGLQIIASMLLMEEKHRQMEYKNVLEQYRSK 334

Query: 308 AEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLT 367
           AE+YLC CLNKN N   N+KRTPGGLLY R+WNN+QYVS AAFLLT+YSDHLQASNQRL 
Sbjct: 335 AEYYLCACLNKNNNVTNNVKRTPGGLLYIRQWNNMQYVSNAAFLLTVYSDHLQASNQRLR 394

Query: 368 CHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGF 427
           C Q +VGP+E+  FAKSQVDYILG NPMAMSYLVG+G RYPQR+HHRGASIES K N GF
Sbjct: 395 CDQGEVGPEEIFAFAKSQVDYILGANPMAMSYLVGYGLRYPQRMHHRGASIESYKENRGF 454

Query: 428 IGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFA 487
           IGCTQGYDNW+ R DPNPN+++G +VGGP++ D+F D+R N+MQTEACTYNTA LVGV A
Sbjct: 455 IGCTQGYDNWFRRKDPNPNIVVGALVGGPDQMDQFSDDRENFMQTEACTYNTASLVGVLA 514

BLAST of Carg27288 vs. TAIR10
Match: AT2G32990.1 (glycosyl hydrolase 9B8)

HSP 1 Score: 724.2 bits (1868), Expect = 5.5e-209
Identity = 330/467 (70.66%), Postives = 392/467 (83.94%), Query Frame = 0

Query: 21  DYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNIKFGL 80
           DY  ALS +LLYFE+Q SGRLPYNQR+ WR HS L+DGL QGVDLVGGY+DAGD++KFGL
Sbjct: 39  DYGEALSKSLLYFEAQRSGRLPYNQRVTWRDHSGLTDGLEQGVDLVGGYHDAGDHVKFGL 98

Query: 81  PMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATD 140
           PMAFTVTMLSW VIEYG+ LA+ GE  HALEAIKWGTDYFIKAH  PNV WA+VGDG TD
Sbjct: 99  PMAFTVTMLSWSVIEYGDSLASTGELSHALEAIKWGTDYFIKAHTSPNVLWAEVGDGDTD 158

Query: 141 HYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLHHAQE 200
           HYCWQRPEDMTTSR+A+KIDENNPGSD+AGETAAAMAAA+IVF  TNPHYSH+LLHHAQ+
Sbjct: 159 HYCWQRPEDMTTSRRAFKIDENNPGSDIAGETAAAMAAASIVFRSTNPHYSHLLLHHAQQ 218

Query: 201 LFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLENGKNF 260
           LFEFG+K+RGKYDES+K  K YY+S SGY DELLW A WLY+++  E Y+ YV++     
Sbjct: 219 LFEFGDKYRGKYDESLKVVKSYYASVSGYMDELLWGATWLYRATDNEHYMSYVVDMAHQL 278

Query: 261 GGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRAKAEHYLCGCLNKNKNN 320
           GG++W ++EFSWDVK+AG+Q+L SML  EE+ K+   +++ Y++KA+HYLC  LNKN N 
Sbjct: 279 GGLSWAMSEFSWDVKFAGVQLLASMLLKEEKHKQHSKVLQQYKSKADHYLCSILNKNING 338

Query: 321 LTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLTCHQHKVGPQEMLTFA 380
            TN++RTP GLLY R+WNN+QYVSTA+FLLT+YSDHL+ SN  L CH+  V P EML FA
Sbjct: 339 -TNVQRTPAGLLYVRQWNNMQYVSTASFLLTVYSDHLRKSNTDLECHEGTVTPDEMLGFA 398

Query: 381 KSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCTQGYDNWYARLD 440
           KSQ+DYILG NPM  SYLVG+GP+YP RVHHRGASI S K + GFIGCTQGYDNWY R +
Sbjct: 399 KSQIDYILGSNPMETSYLVGYGPKYPIRVHHRGASIASFKEHKGFIGCTQGYDNWYGRSE 458

Query: 441 PNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFAKL 488
           PNP+VL+G +VGGP+ +D+F D R NY+QTEACTYNTAPLVGVFA+L
Sbjct: 459 PNPSVLVGALVGGPDHQDDFDDRRGNYVQTEACTYNTAPLVGVFARL 504

BLAST of Carg27288 vs. TAIR10
Match: AT1G64390.1 (glycosyl hydrolase 9C2)

HSP 1 Score: 595.1 bits (1533), Expect = 3.9e-170
Identity = 279/484 (57.64%), Postives = 357/484 (73.76%), Query Frame = 0

Query: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64
           L+  LL  P   S  HDY  ALS +LL+FE+Q SG LP NQR+ WR HS L+DG   GV+
Sbjct: 10  LLLLLLCFPVAFS-GHDYGQALSKSLLFFEAQRSGVLPRNQRVTWRSHSGLTDGKSSGVN 69

Query: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124
           LVGGYYDAGDN+KFGLPMAFTVTM++W VIEYG QL A GE  ++++AIKWGTDYFIKAH
Sbjct: 70  LVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGNQLQANGELGNSIDAIKWGTDYFIKAH 129

Query: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184
           P PNV + +VGDG TDHYCWQRPE+MTT R+AY+ID +NPGSD+AGETAAAMAAA+IVF 
Sbjct: 130 PEPNVLYGEVGDGNTDHYCWQRPEEMTTDRKAYRIDPSNPGSDLAGETAAAMAAASIVFR 189

Query: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244
           R+NP YS +LL HA +LF+F +K+RGKYD SI  A+ YY S SGY DELLWAA WLY++S
Sbjct: 190 RSNPVYSRLLLTHAYQLFDFADKYRGKYDSSITVAQKYYRSVSGYNDELLWAAAWLYQAS 249

Query: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304
             + YL Y+  NG   GG  W + EF WDVKYAG+Q LV+   ++ +      +   Y+ 
Sbjct: 250 NNQFYLDYLGRNGDAMGGTGWSMTEFGWDVKYAGVQTLVAKFLMQGKAGRHAPVFRKYQE 309

Query: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364
           KA+ ++C  L K+     N+++TPGGL++ ++WNN+Q+V++A+FL T+YSD+L +S   L
Sbjct: 310 KADSFMCSLLGKSSR---NIQKTPGGLIFRQRWNNMQFVTSASFLTTVYSDYLTSSRSNL 369

Query: 365 TCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGG 424
            C    V P ++L+FAKSQVDYILGDNP A SY+VG+G  +PQRVHHRG+SI S+K +  
Sbjct: 370 RCAAGNVAPSQLLSFAKSQVDYILGDNPRATSYMVGYGNNFPQRVHHRGSSIVSVKVDRT 429

Query: 425 FIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVF 484
           F+ C  GY  W++R   +PN+L G +VGGP+  D F D R NY QTE  TYN APL+GV 
Sbjct: 430 FVTCRGGYATWFSRKGSDPNLLTGAIVGGPDAYDNFADRRDNYEQTEPATYNNAPLLGVL 489

Query: 485 AKLS 489
           A+LS
Sbjct: 490 ARLS 489

BLAST of Carg27288 vs. TAIR10
Match: AT4G11050.1 (glycosyl hydrolase 9C3)

HSP 1 Score: 575.1 bits (1481), Expect = 4.1e-164
Identity = 272/483 (56.31%), Postives = 349/483 (72.26%), Query Frame = 0

Query: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64
           L+  LL L       HDY  ALS ++L+FE+Q SG LP NQR+ WR HS L DG   GVD
Sbjct: 10  LVVLLLGLVQLAISGHDYKQALSKSILFFEAQRSGHLPPNQRVSWRSHSGLYDGKSSGVD 69

Query: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124
           LVGGYYDAGDN+KFGLPMAFTVT + W +IEYG QL + GE  HA++A+KWGTDYFIKAH
Sbjct: 70  LVGGYYDAGDNVKFGLPMAFTVTTMCWSIIEYGGQLESNGELGHAIDAVKWGTDYFIKAH 129

Query: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184
           P PNV + +VGDG +DHYCWQRPE+MTT R+AYKID NNPGSD+AGETAAAMAAA+IVF 
Sbjct: 130 PEPNVLYGEVGDGKSDHYCWQRPEEMTTDRRAYKIDRNNPGSDLAGETAAAMAAASIVFR 189

Query: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244
           R++P YS  LL HA +LFEF +K+RGKYD SI  A+ YY S SGY DELLWAA WLY+++
Sbjct: 190 RSDPSYSAELLRHAHQLFEFADKYRGKYDSSITVAQKYYRSVSGYNDELLWAAAWLYQAT 249

Query: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304
             + YL Y+ +NG + G           DVKYAG+Q LV+ + ++ +  E  ++ E Y+ 
Sbjct: 250 NDKYYLDYLGKNGDSMGXXXXXXXXXXXDVKYAGVQTLVAKVLMQGKGGEHTAVFERYQQ 309

Query: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364
           KAE ++C  L K+     N+K+TPGGL++ + WNN+Q+V++A+FL T+YSD+L  S + L
Sbjct: 310 KAEQFMCSLLGKSTK---NIKKTPGGLIFRQSWNNMQFVTSASFLATVYSDYLSYSKRDL 369

Query: 365 TCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGG 424
            C Q  + P ++L F+KSQVDYILGDNP A SY+VG+G  YP++VHHRG+SI S   +  
Sbjct: 370 LCSQGNISPSQLLEFSKSQVDYILGDNPRATSYMVGYGENYPRQVHHRGSSIVSFNVDQK 429

Query: 425 FIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVF 484
           F+ C  GY  W++R   +PNVL G +VGGP+  D F D+R NY QTE  TYN APL+GV 
Sbjct: 430 FVTCRGGYATWFSRKGSDPNVLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPLLGVL 489

Query: 485 AKL 488
           A+L
Sbjct: 490 ARL 489

BLAST of Carg27288 vs. TAIR10
Match: AT1G48930.1 (glycosyl hydrolase 9C1)

HSP 1 Score: 560.8 bits (1444), Expect = 8.1e-160
Identity = 267/483 (55.28%), Postives = 345/483 (71.43%), Query Frame = 0

Query: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64
           L+S LL      +  ++Y  AL  T L+FE+Q SG+LP  QR+ WR  S L DGL QGV 
Sbjct: 14  LLSVLLAAATAAAEYYNYGSALDKTFLFFEAQRSGKLPAAQRVKWRGPSGLKDGLAQGVS 73

Query: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124
           L GGYYDAGD++KFGLPMAF VTMLSW  ++  ++L+++ +    L +I+WGTDYFIKAH
Sbjct: 74  LEGGYYDAGDHVKFGLPMAFAVTMLSWAAVDNRKELSSSNQMQQTLWSIRWGTDYFIKAH 133

Query: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184
           P PNV W QVGDG +DHYCW+RPEDMTTSR AYK+D  +PGSD+AGETAAA+AAA++ F 
Sbjct: 134 PQPNVLWGQVGDGESDHYCWERPEDMTTSRTAYKLDPYHPGSDLAGETAAALAAASLAFK 193

Query: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244
             N  YS +LL HA+ELF F +K+RG Y  SI  AK +Y S SGY DELLWAA WL++++
Sbjct: 194 PFNSSYSALLLSHAKELFSFADKYRGLYTNSIPNAKAFYMS-SGYSDELLWAAAWLHRAT 253

Query: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304
           G + YLKY ++N    GG  WG+ EFSWD KYAG+QIL+S + +E +     S ++ Y+ 
Sbjct: 254 GDQYYLKYAMDNSGYMGGTGWGVKEFSWDNKYAGVQILLSKILLEGKGGIYTSTLKQYQT 313

Query: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364
           KA+++ C CL KN     N++ TPGGL+Y R+WNNLQY S AA+LL +YSD+L A+N +L
Sbjct: 314 KADYFACACLKKNGG--YNIQTTPGGLMYVREWNNLQYASAAAYLLAVYSDYLSAANAKL 373

Query: 365 TCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGG 424
            C    V PQ +L FA+SQ DYILG N   MSY+VG+GP+YP RVHHRG+SI SI     
Sbjct: 374 NCPDGLVQPQGLLDFARSQADYILGKNRQGMSYVVGYGPKYPIRVHHRGSSIPSIFAQRS 433

Query: 425 FIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVF 484
            + C QG+D+WY R   +PNV+ G +VGGP+E D + D+RSNY Q+E     TAPLVG+F
Sbjct: 434 SVSCVQGFDSWYRRSQGDPNVIYGALVGGPDENDNYSDDRSNYEQSEPTLSGTAPLVGLF 493

Query: 485 AKL 488
           AKL
Sbjct: 494 AKL 493

BLAST of Carg27288 vs. TAIR10
Match: AT2G44550.1 (glycosyl hydrolase 9B10)

HSP 1 Score: 495.4 bits (1274), Expect = 4.2e-140
Identity = 244/469 (52.03%), Postives = 320/469 (68.23%), Query Frame = 0

Query: 17  SLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNI 76
           ++S +Y  AL  +LLYFE+Q SG+LP NQR+ WR  SAL DG    +DL GGYYDAGDN+
Sbjct: 29  AVSTNYAEALKNSLLYFEAQRSGKLPPNQRVTWRGDSALRDGSDAHIDLTGGYYDAGDNM 88

Query: 77  KFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGD 136
           KFG P+AFT TML+W  IE   QL A  E  +AL A+KW TDY IKAHP PNV + QVG+
Sbjct: 89  KFGFPLAFTTTMLAWSNIEMASQLRAHHEKGNALRALKWATDYLIKAHPQPNVLYGQVGE 148

Query: 137 GATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLH 196
           G +DH CW RPEDMTT R +Y+ID  +PGSD+AGETAAAMAAA+I F+ ++  Y+++L+ 
Sbjct: 149 GNSDHKCWMRPEDMTTPRTSYRIDAQHPGSDLAGETAAAMAAASIAFAPSDKAYANILIG 208

Query: 197 HAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLEN 256
           HA++LF F   HRG Y  SI  A G+Y+S SGY+DELLWAA WL++++  + YL Y+ E 
Sbjct: 209 HAKDLFAFAKAHRGLYQNSIPNAGGFYAS-SGYEDELLWAAAWLHRATNDQIYLDYLTE- 268

Query: 257 GKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRAKAEHYLCGCLNK 316
               GG       F+WD K+ G Q+LV+ L +E + +  + I+E Y++ AE ++C C  K
Sbjct: 269 -AETGGPR---TVFAWDDKFVGAQVLVAKLALEGKVESSEQIVE-YKSMAEQFICNCAQK 328

Query: 317 NKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLTCHQHKVGPQEM 376
             N   N+K+TPGGLLY   WNNLQY + A F+L+ YS +L+A+   + C    +   ++
Sbjct: 329 GDN---NVKKTPGGLLYFLPWNNLQYTTAATFVLSAYSKYLEAAKASIDCPDGALQASDL 388

Query: 377 LTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCTQGYDNWY 436
           L  A+SQVDYILG NP  MSY+VG G  YP++ HHR ASI SI+ +   + C+ GYD WY
Sbjct: 389 LQVARSQVDYILGSNPQKMSYMVGVGTNYPKKPHHRAASIVSIRQDKTPVTCSGGYDKWY 448

Query: 437 ARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFA 486
               PNPNVL G VVGGP++ D + DERSN+ Q E  T  TAPLVGV A
Sbjct: 449 NNPAPNPNVLAGAVVGGPDDNDVYGDERSNFQQAEPATVTTAPLVGVLA 487

BLAST of Carg27288 vs. Swiss-Prot
Match: sp|O48766|GUN11_ARATH (Endoglucanase 11 OS=Arabidopsis thaliana OX=3702 GN=At2g32990 PE=2 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 9.9e-208
Identity = 330/467 (70.66%), Postives = 392/467 (83.94%), Query Frame = 0

Query: 21  DYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNIKFGL 80
           DY  ALS +LLYFE+Q SGRLPYNQR+ WR HS L+DGL QGVDLVGGY+DAGD++KFGL
Sbjct: 39  DYGEALSKSLLYFEAQRSGRLPYNQRVTWRDHSGLTDGLEQGVDLVGGYHDAGDHVKFGL 98

Query: 81  PMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATD 140
           PMAFTVTMLSW VIEYG+ LA+ GE  HALEAIKWGTDYFIKAH  PNV WA+VGDG TD
Sbjct: 99  PMAFTVTMLSWSVIEYGDSLASTGELSHALEAIKWGTDYFIKAHTSPNVLWAEVGDGDTD 158

Query: 141 HYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLHHAQE 200
           HYCWQRPEDMTTSR+A+KIDENNPGSD+AGETAAAMAAA+IVF  TNPHYSH+LLHHAQ+
Sbjct: 159 HYCWQRPEDMTTSRRAFKIDENNPGSDIAGETAAAMAAASIVFRSTNPHYSHLLLHHAQQ 218

Query: 201 LFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLENGKNF 260
           LFEFG+K+RGKYDES+K  K YY+S SGY DELLW A WLY+++  E Y+ YV++     
Sbjct: 219 LFEFGDKYRGKYDESLKVVKSYYASVSGYMDELLWGATWLYRATDNEHYMSYVVDMAHQL 278

Query: 261 GGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRAKAEHYLCGCLNKNKNN 320
           GG++W ++EFSWDVK+AG+Q+L SML  EE+ K+   +++ Y++KA+HYLC  LNKN N 
Sbjct: 279 GGLSWAMSEFSWDVKFAGVQLLASMLLKEEKHKQHSKVLQQYKSKADHYLCSILNKNING 338

Query: 321 LTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLTCHQHKVGPQEMLTFA 380
            TN++RTP GLLY R+WNN+QYVSTA+FLLT+YSDHL+ SN  L CH+  V P EML FA
Sbjct: 339 -TNVQRTPAGLLYVRQWNNMQYVSTASFLLTVYSDHLRKSNTDLECHEGTVTPDEMLGFA 398

Query: 381 KSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCTQGYDNWYARLD 440
           KSQ+DYILG NPM  SYLVG+GP+YP RVHHRGASI S K + GFIGCTQGYDNWY R +
Sbjct: 399 KSQIDYILGSNPMETSYLVGYGPKYPIRVHHRGASIASFKEHKGFIGCTQGYDNWYGRSE 458

Query: 441 PNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFAKL 488
           PNP+VL+G +VGGP+ +D+F D R NY+QTEACTYNTAPLVGVFA+L
Sbjct: 459 PNPSVLVGALVGGPDHQDDFDDRRGNYVQTEACTYNTAPLVGVFARL 504

BLAST of Carg27288 vs. Swiss-Prot
Match: sp|Q6H3Z9|GUN22_ORYSJ (Endoglucanase 22 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU11 PE=2 SV=2)

HSP 1 Score: 666.0 bits (1717), Expect = 3.2e-190
Identity = 303/473 (64.06%), Postives = 375/473 (79.28%), Query Frame = 0

Query: 21  DYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNIKFGL 80
           DY  AL  +LLYFE+Q SGRLPY+QR+ WR HS L+DGL QGVDLVGGYYDAGD++KFGL
Sbjct: 55  DYREALEKSLLYFEAQRSGRLPYSQRVTWRGHSGLTDGLQQGVDLVGGYYDAGDHVKFGL 114

Query: 81  PMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATD 140
           PMAFTVTMLSWG I++   +AAAGE+ HALEAIKWGTDYF+KAH HP V+WA+VGDG TD
Sbjct: 115 PMAFTVTMLSWGAIDFAADIAAAGEWRHALEAIKWGTDYFVKAHTHPFVYWAEVGDGDTD 174

Query: 141 HYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLHHAQE 200
           HYCWQRPEDMTTSRQAY++D +NPGSD+AGETAAA+AAA+IVF R++PHYSH+LLHHAQ+
Sbjct: 175 HYCWQRPEDMTTSRQAYRVDRDNPGSDLAGETAAALAAASIVFRRSDPHYSHLLLHHAQQ 234

Query: 201 LFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLENGKNF 260
           LFEFG+ +RG YD SI+E + YY+S SGY DELLWAA+WL++++G+EEYL+Y ++N  +F
Sbjct: 235 LFEFGDTYRGSYDSSIEEVRSYYASVSGYHDELLWAALWLHRATGKEEYLRYAVDNADSF 294

Query: 261 GGITWGINEFSWDVKYAGLQILVSMLRVEEQPK--EQKSIIEAYRAKAEHYLCGCLNKNK 320
           GG+ W I EFSWDVKYAGLQ+L + L ++  P+    + ++E YR KAEHYLC CL +N 
Sbjct: 295 GGVGWAITEFSWDVKYAGLQVLAAKLLLDGDPQAAAHRGVLEKYREKAEHYLCACLGRNI 354

Query: 321 NNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYS----DHLQASNQRLTCHQHKVGPQ 380
           N   N+ R+PGG+LY R+WNNLQY S+AAFLLT YS                        
Sbjct: 355 NGADNVDRSPGGMLYVRQWNNLQYASSAAFLLTAYSXXXXXXXXXXXXXXXXXXXXXXXX 414

Query: 381 EMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCTQGYDN 440
           EM++ A+SQ DYILG NP+ +SY+VG+G RYP RVHHRGASI S K +G FIGC QG+D+
Sbjct: 415 EMVSLARSQADYILGRNPLRLSYMVGYGRRYPARVHHRGASIVSHKEDGRFIGCVQGFDD 474

Query: 441 WYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFAKL 488
           W+ R   NPNVL G +VGGP+ +DEF D+R+NYMQTEACTYNTAP+V VFA+L
Sbjct: 475 WFGRGRANPNVLAGAIVGGPSRRDEFRDDRANYMQTEACTYNTAPMVAVFARL 527

BLAST of Carg27288 vs. Swiss-Prot
Match: sp|Q6Z5P2|GUN7_ORYSJ (Endoglucanase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU10 PE=2 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 2.5e-187
Identity = 304/476 (63.87%), Postives = 372/476 (78.15%), Query Frame = 0

Query: 19  SHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNIKF 78
           +H+Y  AL  +LLYFE+Q SGRLP+NQR+ WR HS L+DGL QGVDLVGGYYDAGD++KF
Sbjct: 34  AHNYEDALRKSLLYFEAQRSGRLPHNQRVAWRDHSGLTDGLEQGVDLVGGYYDAGDHVKF 93

Query: 79  GLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGDGA 138
           GLPMAFTVTMLSW +IEYG+ + AAGE  HALEAIKWGTDYFIKAH  PN  WA+VGDG 
Sbjct: 94  GLPMAFTVTMLSWSMIEYGDDVEAAGELGHALEAIKWGTDYFIKAHTKPNELWAEVGDGD 153

Query: 139 TDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLHHA 198
           TDHYCWQRPEDMTTSRQAYK+D   PGSDVAGETAAAMAAA+IVF ++NPHY+ +LLHHA
Sbjct: 154 TDHYCWQRPEDMTTSRQAYKVDRERPGSDVAGETAAAMAAASIVFRKSNPHYASLLLHHA 213

Query: 199 QELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLENGK 258
           Q+LFEF +K+RGKYD SI E K YY+S SGYKDELLWAA+WL++++G+  YL YV++N  
Sbjct: 214 QQLFEFADKYRGKYDSSIAEVKSYYASVSGYKDELLWAALWLHRATGKAHYLDYVVDNAD 273

Query: 259 NFGGITWGINEFSWDVKYAGLQILVSMLRVE-EQPKEQKSIIEAYRAKAEHYLCGCLNKN 318
            FGG  W I EFSWDVKYAG+QIL + L +  E  +  +S +E YRAKAEHY+CGCL +N
Sbjct: 274 CFGGTGWAITEFSWDVKYAGVQILAARLLLRGEHEERHRSTLEQYRAKAEHYVCGCLGRN 333

Query: 319 KNN--LTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHL--QASNQRLTCHQHKVGP 378
            +     N++R+PGG+LY R+WNN+QYV+ AAFLL  Y+D+L   A            G 
Sbjct: 334 ADGGADANVERSPGGMLYVRQWNNMQYVTNAAFLLAAYADYLGDDADGAVSCAGGETAGA 393

Query: 379 QEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCTQGYD 438
            E+   A++QVDY+LG NP  +SYLVG+G +YP RVHHR ASI   K +  FIGCTQG+D
Sbjct: 394 GEVAALARAQVDYVLGTNPRGISYLVGYGAKYPNRVHHRAASIVPYKHSKEFIGCTQGFD 453

Query: 439 NWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFAKLSQ 490
           +W+ R   NPNVL+G +VGGP+ +D F D R NYMQTEACTYNTAP+VG+FAKL++
Sbjct: 454 HWFGRRSSNPNVLVGAIVGGPDRRDRFRDNRENYMQTEACTYNTAPMVGMFAKLNR 509

BLAST of Carg27288 vs. Swiss-Prot
Match: sp|Q67UW5|GUN5_ORYSJ (Endoglucanase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0151300 PE=2 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 4.8e-186
Identity = 309/484 (63.84%), Postives = 371/484 (76.65%), Query Frame = 0

Query: 20  HDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNIKFG 79
           HDY  ALS +LLYFE+Q SGRLPYNQR+ WR HS L+DGL QGVDLVGGYYDAGD++KFG
Sbjct: 28  HDYGEALSKSLLYFEAQRSGRLPYNQRVRWRGHSGLTDGLEQGVDLVGGYYDAGDHVKFG 87

Query: 80  LPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGDGAT 139
           LPMAFTVTMLSW V+EYGE++AAAGE  HAL AIKWGTDYFIKAH HPNV W QVGDG +
Sbjct: 88  LPMAFTVTMLSWSVLEYGEEIAAAGELGHALHAIKWGTDYFIKAHTHPNVLWTQVGDGDS 147

Query: 140 DHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRT-NPHYSHVLLHHA 199
           DHYCWQRPEDMTTSR AYK+D  NPGS+VA ETAAAMAAA+IVF R  + HY+H+LLHHA
Sbjct: 148 DHYCWQRPEDMTTSRHAYKVDAENPGSEVAAETAAAMAAASIVFRRAGDAHYAHLLLHHA 207

Query: 200 QELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLENGK 259
           Q+LFEFG+K+RG+YDES++  K YY S+SGYKDELLWAA+WL++++GR EYL Y ++N  
Sbjct: 208 QQLFEFGDKYRGRYDESVEVVKNYYPSSSGYKDELLWAALWLHRATGRREYLDYAVDNAD 267

Query: 260 NFGGITWGINEFSWDVKYAGLQILVSMLRVEEQ--PKEQKSIIEAYRAKAEHYLCGCLNK 319
           +FGG  W ++EFSWD+KYAGLQ+L S L VEE+    +Q+ ++E YR+KAE+Y+C C+ +
Sbjct: 268 DFGGTGWAVSEFSWDIKYAGLQVLASKLLVEEKHLSSQQREVLEKYRSKAEYYVCSCMGR 327

Query: 320 NKNNLT-NLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLTCHQHKVGPQ- 379
           N      N  RTP GLL+ R WNNLQYVS AAFLLT+YSD L   +  L C         
Sbjct: 328 NPGGAAHNAGRTPAGLLFIRPWNNLQYVSNAAFLLTVYSDVLSYLSLPLLCPDPDAAADE 387

Query: 380 ---------EMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGF 439
                    E+L FA+SQ DYILG NPMA SYLVG+G  YP+RVHHR AS  S   +  F
Sbjct: 388 AAPAAADAGEVLEFARSQADYILGTNPMATSYLVGYGEAYPRRVHHRAASSASYARDRDF 447

Query: 440 IGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFA 490
           IGC QG+D+WY+    NP+ L+G VVGGPN  D F D R  YMQTEACTYNTAP+VGVF+
Sbjct: 448 IGCLQGFDSWYSAAAENPHDLVGAVVGGPNGNDVFTDHRGAYMQTEACTYNTAPMVGVFS 507

BLAST of Carg27288 vs. Swiss-Prot
Match: sp|Q654U4|GUN16_ORYSJ (Endoglucanase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0247900 PE=2 SV=2)

HSP 1 Score: 628.6 bits (1620), Expect = 5.7e-179
Identity = 292/480 (60.83%), Postives = 358/480 (74.58%), Query Frame = 0

Query: 22  YHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNIKFGLP 81
           Y  AL  +LLYFE+Q SGRLP+ QR+ WR HS L+DGL QGVDLVGGYYDAGD++KFGLP
Sbjct: 31  YEEALRKSLLYFEAQRSGRLPHGQRVAWRDHSGLTDGLEQGVDLVGGYYDAGDHVKFGLP 90

Query: 82  MAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGDGATDH 141
           MAFTVTMLSW ++EYG  +AAAGE  HAL+AIKWGTDYFIKAH  P+  WA+VGDG TDH
Sbjct: 91  MAFTVTMLSWSLLEYGADVAAAGELAHALDAIKWGTDYFIKAHTKPHELWAEVGDGDTDH 150

Query: 142 YCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLHHAQEL 201
           YCWQRPEDMTTSRQAYK+D   PGSDVAGETAAAMAAA+IVF ++NPHYSH+LLHHAQ+L
Sbjct: 151 YCWQRPEDMTTSRQAYKVDRRRPGSDVAGETAAAMAAASIVFRQSNPHYSHLLLHHAQQL 210

Query: 202 FEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLENGKNFG 261
           FEF + +RGKYD SI E K YY+S SGY DELLWAA+WL++++GR  YL Y ++N   FG
Sbjct: 211 FEFADTYRGKYDSSIAEVKSYYASVSGYHDELLWAALWLHRATGRAAYLDYAVDNADEFG 270

Query: 262 GITWGINEFSWDVKYAGLQILVSMLRVE-EQPKEQKSIIEAYRAKAEHYLCGCLNKNK-- 321
           G  W I EFSWDVKYAG+QIL + L +  E  +  +  +E YR KAEHY+C C+ +N   
Sbjct: 271 GTGWAITEFSWDVKYAGVQILAARLLMRGEHEERHRGTLERYREKAEHYVCACMGRNAAG 330

Query: 322 NNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQA---------SNQRLTCHQH 381
               N++R+PGG+LY R+WNN+QYV+ AAFLL+ YSD+L                     
Sbjct: 331 GADANVERSPGGMLYVRQWNNMQYVTNAAFLLSAYSDYLAGXXXXXXXXXXXXXXXXXXX 390

Query: 382 KVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCT 441
                 +   A+ QVDY+LG NP  MSYLVG+G R+P RVHHR ASI   K +  FIGC 
Sbjct: 391 XXXXXXVFAAAREQVDYVLGSNPRGMSYLVGYGERFPARVHHRAASIVPYKDSKEFIGCA 450

Query: 442 QGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFAKLSQ 490
           QG+D+W+ R   NPNV++G +VGGP+ +D F D+R NYMQTEACTYNTAP+VG+FA L++
Sbjct: 451 QGFDDWFGRRGANPNVVVGAIVGGPDRRDRFRDDRENYMQTEACTYNTAPMVGMFAMLNR 510

BLAST of Carg27288 vs. TrEMBL
Match: tr|A0A2I4DY32|A0A2I4DY32_9ROSI (Endoglucanase OS=Juglans regia OX=51240 GN=LOC108984524 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 6.2e-223
Identity = 361/483 (74.74%), Postives = 417/483 (86.34%), Query Frame = 0

Query: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64
           L+S   + P +  LS DY  ALS +LLYFESQ SGRLP+NQR+ WRHHS L+DGL QGVD
Sbjct: 23  LLSIFSLFP-SSCLSFDYGEALSKSLLYFESQRSGRLPHNQRVSWRHHSGLTDGLEQGVD 82

Query: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124
           LVGGYYDAGDN+KFGLPMAFTVTMLSW V+EYGEQ+A AGEY+HALEAIKWGTDYFIKAH
Sbjct: 83  LVGGYYDAGDNVKFGLPMAFTVTMLSWSVLEYGEQIAGAGEYLHALEAIKWGTDYFIKAH 142

Query: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184
            HPNV WAQVG+G TDHYCWQRPEDMTTSRQAYK+D NNPGSD+AGETAAAMAAA++VF 
Sbjct: 143 THPNVLWAQVGNGDTDHYCWQRPEDMTTSRQAYKVDANNPGSDIAGETAAAMAAASMVFR 202

Query: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244
           +TNPHYSH+L+HHAQ+LFEFG+K+RGKYDESIK +KGYYSS SGY DELLWAA+WLYK++
Sbjct: 203 KTNPHYSHLLIHHAQQLFEFGDKYRGKYDESIKVSKGYYSSVSGYMDELLWAALWLYKAT 262

Query: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304
            +EEYL YVLEN  +FGGITW + EFSWDVKY G+QI+ SML +E++ ++ K ++E YR+
Sbjct: 263 DKEEYLNYVLENAHSFGGITWAMTEFSWDVKYVGVQIIASMLLMEKKHEKHKHVLEQYRS 322

Query: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364
           KAEH+LC CL  N NN TN++RTPGGLLYTR+WNN+QYVSTAAFLLTLYSD+L+ SNQ L
Sbjct: 323 KAEHFLCACL--NMNNATNVQRTPGGLLYTRQWNNMQYVSTAAFLLTLYSDYLRTSNQEL 382

Query: 365 TCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGG 424
            CH   +GPQE+ +FAKSQVDYILG NP A+SYLVG+G  YP RVHHRGASIES K N G
Sbjct: 383 NCHNGVLGPQEIFSFAKSQVDYILGSNPKALSYLVGYGSNYPYRVHHRGASIESYKRNQG 442

Query: 425 FIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVF 484
           FIGCTQGYDNWY R DPNPN L+G +VGGP+ KD F DERSNY+QTEACTYN AP+VGVF
Sbjct: 443 FIGCTQGYDNWYGRHDPNPNTLVGALVGGPDNKDRFKDERSNYIQTEACTYNVAPVVGVF 502

Query: 485 AKL 488
           AKL
Sbjct: 503 AKL 502

BLAST of Carg27288 vs. TrEMBL
Match: tr|A0A1R3KBD8|A0A1R3KBD8_9ROSI (Endoglucanase OS=Corchorus olitorius OX=93759 GN=COLO4_09710 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 3.1e-222
Identity = 361/482 (74.90%), Postives = 417/482 (86.51%), Query Frame = 0

Query: 8   FLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVG 67
           FL ILP T     DY  ALS +LLYFESQ SGRLPYNQR+ WRHHS L+DGL QGVDLVG
Sbjct: 35  FLAILPLTQPF--DYGKALSKSLLYFESQRSGRLPYNQRVTWRHHSGLTDGLEQGVDLVG 94

Query: 68  GYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHP 127
           GYYDAGDN+KFGLPMAFT+TMLSWGVI+YG+++A AGEY HALEAIKWGTDYFIKAH HP
Sbjct: 95  GYYDAGDNVKFGLPMAFTITMLSWGVIDYGDEIAGAGEYSHALEAIKWGTDYFIKAHTHP 154

Query: 128 NVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTN 187
           NV WA+VGDG TDHYCWQRPEDMTTSRQAYK+DE NPGSDVAGETAAAMA+A+IVF +TN
Sbjct: 155 NVLWAEVGDGDTDHYCWQRPEDMTTSRQAYKVDEKNPGSDVAGETAAAMASASIVFRKTN 214

Query: 188 PHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGRE 247
           PHYSH+LL HA++LFEFG+K+RGKYDES+K  KGYYSS SG+ DELLWAA+WLYK++ +E
Sbjct: 215 PHYSHLLLQHAKQLFEFGDKYRGKYDESVKVVKGYYSSVSGFTDELLWAALWLYKATDKE 274

Query: 248 EYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPK--EQKSIIEAYRAK 307
           +YL+Y LE    FGGITW INEFSWDVK+AGLQI+ SML +EE+ +  E K+++E YR+K
Sbjct: 275 DYLRYALEKAYEFGGITWAINEFSWDVKFAGLQIIASMLLMEEKHRQMEYKNVLEQYRSK 334

Query: 308 AEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLT 367
           AE+YLC CLNKN N   N+KRTPGGLLY R+WNN+QYVS AAFLLT+YSDHLQASNQRL 
Sbjct: 335 AEYYLCACLNKNNNVTNNVKRTPGGLLYIRQWNNMQYVSNAAFLLTVYSDHLQASNQRLR 394

Query: 368 CHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGF 427
           C Q +VGP+E+  FAKSQVDYILG NPMAMSYLVG+G RYPQR+HHRGASIES K N GF
Sbjct: 395 CDQGEVGPEEIFAFAKSQVDYILGANPMAMSYLVGYGLRYPQRMHHRGASIESYKENRGF 454

Query: 428 IGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFA 487
           IGCTQGYDNW+ R DPNPN+++G +VGGP++ D+F D+R N+MQTEACTYNTA LVGV A
Sbjct: 455 IGCTQGYDNWFRRKDPNPNIVVGALVGGPDQMDQFSDDRENFMQTEACTYNTASLVGVLA 514

BLAST of Carg27288 vs. TrEMBL
Match: tr|A0A0A0L504|A0A0A0L504_CUCSA (Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G073940 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 3.4e-221
Identity = 375/486 (77.16%), Postives = 414/486 (85.19%), Query Frame = 0

Query: 5   LISFLLILPFTHSLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVD 64
           +I FLLILP THS   +Y  ALSI+LLYFESQ SGRLPYNQRL WRHHS L+DGLHQGVD
Sbjct: 12  MIFFLLILPLTHSF--NYQQALSISLLYFESQRSGRLPYNQRLTWRHHSGLTDGLHQGVD 71

Query: 65  LVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAH 124
           LVGGYYDAGDN+KFGLPMAFTVTMLSWGVIEYGEQ+AAAGEYIHALEAIKWGTDYFIKAH
Sbjct: 72  LVGGYYDAGDNVKFGLPMAFTVTMLSWGVIEYGEQIAAAGEYIHALEAIKWGTDYFIKAH 131

Query: 125 PHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFS 184
           PHPNV WAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAI   
Sbjct: 132 PHPNVLWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAI--- 191

Query: 185 RTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSS 244
                           LFEFG+K+RGKYDESI+  KGYY SASGY DELLWAA WLYK+S
Sbjct: 192 ----------------LFEFGDKYRGKYDESIEVVKGYYPSASGYMDELLWAATWLYKAS 251

Query: 245 GREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRA 304
           G+EEYLKYV+EN  +FGGI+W INEFSWD+KYA          VEE+ KE+K I+EAY++
Sbjct: 252 GKEEYLKYVIENALDFGGISWAINEFSWDIKYA----------VEEKQKEEKLIVEAYKS 311

Query: 305 KAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRL 364
           KAEHYLC CLNK+  N  NLKRTPGGLLYTRKWNNLQYV+TA FLLTLYSDHL++SNQRL
Sbjct: 312 KAEHYLCSCLNKSSTN-NNLKRTPGGLLYTRKWNNLQYVTTATFLLTLYSDHLESSNQRL 371

Query: 365 TC-HQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNG 424
            C +  +VGP+EML+ AKSQVDYILG+NPM MSYLVGFG RYP+RVHHRGAS+ES+K   
Sbjct: 372 RCSNDDEVGPEEMLSLAKSQVDYILGENPMGMSYLVGFGGRYPERVHHRGASMESVKEKR 431

Query: 425 GFIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGV 484
           GFIGC QGYD+WY R+D NPNVLMG VVGGPNE+DEF+DERSN+ QTEACTYNTAPLVGV
Sbjct: 432 GFIGCVQGYDDWYGRVDGNPNVLMGAVVGGPNERDEFNDERSNFRQTEACTYNTAPLVGV 465

Query: 485 FAKLSQ 490
           FA+ +Q
Sbjct: 492 FARFNQ 465

BLAST of Carg27288 vs. TrEMBL
Match: tr|A0A2C9UDU1|A0A2C9UDU1_MANES (Endoglucanase OS=Manihot esculenta OX=3983 GN=MANES_15G067300 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 2.4e-219
Identity = 364/485 (75.05%), Postives = 414/485 (85.36%), Query Frame = 0

Query: 8   FLLILPFTHSL-----SHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQG 67
           FLL   F   L     S +Y  ALS +LLYFESQ SGRLPYNQR+ WRHHSAL+DGL QG
Sbjct: 11  FLLFFSFAAVLLPVCQSFNYTEALSKSLLYFESQRSGRLPYNQRVTWRHHSALTDGLEQG 70

Query: 68  VDLVGGYYDAGDNIKFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIK 127
           VDLVGGYYDAGDN+KFGLPMAFTVTML+WGVI+Y +Q++ AGEY HALEAIKWGTDYFIK
Sbjct: 71  VDLVGGYYDAGDNVKFGLPMAFTVTMLAWGVIQYEKQMSDAGEYQHALEAIKWGTDYFIK 130

Query: 128 AHPHPNVFWAQVGDGATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIV 187
           AH  PNV W QVGDG TDHYCWQRPEDMTTSRQAYK+DE NPGSDVAGETAAA+AAA+IV
Sbjct: 131 AHTQPNVLWVQVGDGYTDHYCWQRPEDMTTSRQAYKVDEKNPGSDVAGETAAALAAASIV 190

Query: 188 FSRTNPHYSHVLLHHAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYK 247
           F RTNPHYS++LLHHA+ELFEFG+KHRGKYDES+K  KGYY S SGYKDELLWAA+WL+K
Sbjct: 191 FRRTNPHYSYLLLHHAKELFEFGDKHRGKYDESVKAVKGYYPSVSGYKDELLWAALWLHK 250

Query: 248 SSGREEYLKYVLENGKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAY 307
           ++  E YL YVLENG  FGGITW I+EFSWDVKYAG+Q++ SML  +E+ ++ K I++ Y
Sbjct: 251 ATDNEAYLMYVLENGYGFGGITWAISEFSWDVKYAGVQLIASMLLRDERQRKHKLILQKY 310

Query: 308 RAKAEHYLCGCLNKNKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQ 367
           R+KAEHYLC CL  +KNN TN++RTPGGLL+ RKWNNLQYVSTAAFLLT+YSD+L ASNQ
Sbjct: 311 RSKAEHYLCSCL--HKNNATNVERTPGGLLHIRKWNNLQYVSTAAFLLTVYSDYLDASNQ 370

Query: 368 RLTCHQHKVGPQEMLTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTN 427
           RL C Q  + PQE+ +FAKSQVDYILG+NPM MSYLVG+G RYP +VHHRGASIES + N
Sbjct: 371 RLKCDQGMLTPQEIFSFAKSQVDYILGNNPMGMSYLVGYGLRYPLKVHHRGASIESYRGN 430

Query: 428 GGFIGCTQGYDNWYARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVG 487
            GFIGCTQGYD WY+R DPNPNV++G +VGGPN KDEF DER NYMQTEACTYNTA LVG
Sbjct: 431 KGFIGCTQGYDLWYSRQDPNPNVIVGAIVGGPNNKDEFWDERENYMQTEACTYNTATLVG 490

BLAST of Carg27288 vs. TrEMBL
Match: tr|B9SU00|B9SU00_RICCO (Endo-1,4-beta-glucanase, putative OS=Ricinus communis OX=3988 GN=RCOM_0753090 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 4.2e-219
Identity = 359/471 (76.22%), Postives = 406/471 (86.20%), Query Frame = 0

Query: 17  SLSHDYHHALSITLLYFESQLSGRLPYNQRLPWRHHSALSDGLHQGVDLVGGYYDAGDNI 76
           S S +Y  ALS +LLYFESQ SGRLPYNQR+ WRHHS L+DGL QGVDLVGGYYDAGDN+
Sbjct: 24  SQSFNYADALSKSLLYFESQRSGRLPYNQRVTWRHHSGLTDGLEQGVDLVGGYYDAGDNV 83

Query: 77  KFGLPMAFTVTMLSWGVIEYGEQLAAAGEYIHALEAIKWGTDYFIKAHPHPNVFWAQVGD 136
           KFGLPMAFT+TMLSWGVIEYG ++  AGEY HALEAIKWGTDYFIKAH HPNV W QVGD
Sbjct: 84  KFGLPMAFTITMLSWGVIEYGNEIGDAGEYEHALEAIKWGTDYFIKAHTHPNVLWVQVGD 143

Query: 137 GATDHYCWQRPEDMTTSRQAYKIDENNPGSDVAGETAAAMAAAAIVFSRTNPHYSHVLLH 196
           G TDHYCWQRPEDMTTSRQAYK+D NNPGSDVAGETAAA+AAA+IVF +TNPHYSH+LLH
Sbjct: 144 GYTDHYCWQRPEDMTTSRQAYKVDANNPGSDVAGETAAALAAASIVFRKTNPHYSHLLLH 203

Query: 197 HAQELFEFGNKHRGKYDESIKEAKGYYSSASGYKDELLWAAMWLYKSSGREEYLKYVLEN 256
           HAQ+LFEFG+K+RGKYDES+K  KGYY+S SGYKDELLW A+WLYK++  E YL YVLEN
Sbjct: 204 HAQQLFEFGDKYRGKYDESVKVVKGYYTSVSGYKDELLWGALWLYKATDNEAYLMYVLEN 263

Query: 257 GKNFGGITWGINEFSWDVKYAGLQILVSMLRVEEQPKEQKSIIEAYRAKAEHYLCGCLNK 316
              FGGITW I+EFSWDVK+ GLQIL SML  EE+ K+ K I+E Y++KAE+YLC CL  
Sbjct: 264 AHGFGGITWAISEFSWDVKFPGLQILASMLLTEERHKKHKHILEHYQSKAEYYLCSCL-- 323

Query: 317 NKNNLTNLKRTPGGLLYTRKWNNLQYVSTAAFLLTLYSDHLQASNQRLTCHQHKVGPQEM 376
           ++NN+TN+KRTPGGLLY R+WNNLQYVSTAAFLLT+YSDHL ASNQRL C +  + PQE+
Sbjct: 324 DQNNVTNVKRTPGGLLYIRQWNNLQYVSTAAFLLTVYSDHLLASNQRLKCDRGILDPQEI 383

Query: 377 LTFAKSQVDYILGDNPMAMSYLVGFGPRYPQRVHHRGASIESIKTNGGFIGCTQGYDNWY 436
           L+ AKSQ+DYILG NP+ MSYLVG+G  YPQRVHHRGASIES K N GFIGCTQGYD WY
Sbjct: 384 LSVAKSQIDYILGANPVGMSYLVGYGTEYPQRVHHRGASIESYKGNKGFIGCTQGYDMWY 443

Query: 437 ARLDPNPNVLMGGVVGGPNEKDEFHDERSNYMQTEACTYNTAPLVGVFAKL 488
            R DPNPNV++G +VGGP+EKDEF DER NYMQTEACTYNTA LVGV A+L
Sbjct: 444 NRQDPNPNVVVGALVGGPDEKDEFSDERGNYMQTEACTYNTASLVGVLARL 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022932240.12.8e-29197.96endoglucanase 11-like [Cucurbita moschata][more]
XP_023007593.11.2e-28195.31endoglucanase 11-like [Cucurbita maxima][more]
XP_004149358.21.8e-24282.30PREDICTED: endoglucanase 11-like [Cucumis sativus][more]
XP_018812066.19.4e-22374.74PREDICTED: endoglucanase 11-like isoform X2 [Juglans regia][more]
OMP04364.14.7e-22274.90Glycoside hydrolase, family 9 [Corchorus olitorius][more]
Match NameE-valueIdentityDescription
AT2G32990.15.5e-20970.66glycosyl hydrolase 9B8[more]
AT1G64390.13.9e-17057.64glycosyl hydrolase 9C2[more]
AT4G11050.14.1e-16456.31glycosyl hydrolase 9C3[more]
AT1G48930.18.1e-16055.28glycosyl hydrolase 9C1[more]
AT2G44550.14.2e-14052.03glycosyl hydrolase 9B10[more]
Match NameE-valueIdentityDescription
sp|O48766|GUN11_ARATH9.9e-20870.66Endoglucanase 11 OS=Arabidopsis thaliana OX=3702 GN=At2g32990 PE=2 SV=1[more]
sp|Q6H3Z9|GUN22_ORYSJ3.2e-19064.06Endoglucanase 22 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU11 PE=2 SV=2[more]
sp|Q6Z5P2|GUN7_ORYSJ2.5e-18763.87Endoglucanase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU10 PE=2 SV=1[more]
sp|Q67UW5|GUN5_ORYSJ4.8e-18663.84Endoglucanase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0151300 PE=2 SV... [more]
sp|Q654U4|GUN16_ORYSJ5.7e-17960.83Endoglucanase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0247900 PE=2 S... [more]
Match NameE-valueIdentityDescription
tr|A0A2I4DY32|A0A2I4DY32_9ROSI6.2e-22374.74Endoglucanase OS=Juglans regia OX=51240 GN=LOC108984524 PE=3 SV=1[more]
tr|A0A1R3KBD8|A0A1R3KBD8_9ROSI3.1e-22274.90Endoglucanase OS=Corchorus olitorius OX=93759 GN=COLO4_09710 PE=3 SV=1[more]
tr|A0A0A0L504|A0A0A0L504_CUCSA3.4e-22177.16Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G073940 PE=3 SV=1[more]
tr|A0A2C9UDU1|A0A2C9UDU1_MANES2.4e-21975.05Endoglucanase OS=Manihot esculenta OX=3983 GN=MANES_15G067300 PE=3 SV=1[more]
tr|B9SU00|B9SU00_RICCO4.2e-21976.22Endo-1,4-beta-glucanase, putative OS=Ricinus communis OX=3988 GN=RCOM_0753090 PE... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR0089286-hairpin_glycosidase_sf
IPR018221Glyco_hydro_9_His_AS
IPR001701Glyco_hydro_9
IPR0123416hp_glycosidase-like_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg27288-RACarg27288-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012341Six-hairpin glycosidase-like superfamilyGENE3DG3DSA:1.50.10.10coord: 18..490
e-value: 1.8E-170
score: 569.7
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 22..483
e-value: 6.7E-141
score: 470.3
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 5..488
NoneNo IPR availablePANTHERPTHR22298:SF96SUBFAMILY NOT NAMEDcoord: 5..488
IPR018221Glycoside hydrolase family 9, His active sitePROSITEPS00592GLYCOSYL_HYDROL_F9_1coord: 396..412
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILYSSF48208Six-hairpin glycosidasescoord: 20..488