Carg26232 (gene) Silver-seed gourd

NameCarg26232
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionMitotic checkpoint protein bub3
LocationCucurbita_argyrosperma_scaffold_307 : 163304 .. 168120 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTACTTCATTTCCCGCCTCTTTTGTTCGTCTAACCTCGATCCCCAGGCATGGAACCCAACAGGGTTTTGCTGGATTTTCAAAATCCGATCCAAGATGCCATCTCCACAATCCAATTCGCTCCCCGTTCCAACAATCTCCTTATTTCTTCCTGGGATTCTGTTAGTCTTCCTTTTGGTGTTCCAAATTCTTTCAACGTTTCTGCCATTTCTTTTCGTTCACTATGAATGAAGTTCTAGGTTTTGGTTTTTTTGGACAGAGTCTTCGTTTGTACGATGTGGATAATTCTGCACTCAGACTTGAAGCTCCCTCCGAAGCTGCACTGCTTGACTGCTGCTTCCAGGACGAGTCACTGGCTTTGAGCGCTGGTTCTGATGGCTTAATAAGAAGGTTTGGGGTTCGAATTTAATAACAAATCCTCTGGATTAATGTTATGAAATGAAATATCATGGTACAGATTAAGATATTTGGATCTTGCAATTATCTCGATTTGTTCCATTGTTCTTCTTTCAATTCAACATGTTTGGAGAAATTGTTTGTCTATGGCTATAACTATTAAGTTATTGAAATGATTTCCACAAAGTTGGGAAGGAAATTGTCTCTTTCAACTCCCTTCCACCAAATAGGAAAAGTTTCTGAAGTGAAACGTGAGGATTATTTAGATGTTAATGATGGAGTTAAATTCTGTCCTGTTTTCTGCTCTGAAATAGTAAATGCAGTAGTTTGTTGTTCTTCTTTTCATCTCATTCTGTTCTAGATAGTCCATTTTTTTGTCGGTTGATTTCTTAAGAGGATTATTAAAAGAACTACTTCAATTATGCATGAAAACCCCTCTTATTCTAGGTGAAGCCCCACGACAGTCATAAGCATACATGAGTTTAAAAGGTACATATTGTAGTCAAGATAGGAATGATTTTGAGTTTTTAGATTCATTAAGTCTCTTGGATGAATAGTTTCCTTATCAAACTTGAATGAACGCAACTCTCCCATGTAACGACCCAATTATCGATACCTCGAATCACAGGTCGTTACAATTTGATATTAGAGCTAGACATCAAGCAGTGTGCTAGTGAGAACGCTGGACCTCTAAGGGTGTAGATTGTGAGATCCTACATCAGTTGGAGAAGGGAACGAAACATTCCTTACAAGAGTGTAGGAACCTCTCCCTCACAGACGCGTCTTAAAACCTCGTGGGGAAACCCTTGAATGGAAAACCAAAAGAAGATAATATCTGTTAGCGGTGGGCTTGGGCTGTTACAAATGGTATCAGAGTCGTACACTGAGCAGTGTGCCAACGAGGATGCTAGGCCCCCAAGAGGGTTGGATTGTGAGATCTCATATTGGTTGGAGAGGGGAACAAAACATTTCTTATAAATGTGTAGAAACCTCTCCCTAATAGACACGTTTTAAAACCTTGAGGGGAAGCCTTTGAAGGGAAAGTCCAAGGAAGACAATATCTACCAGCGGTGGGCTTGGGCTGTTTCACACCAGCTTGGAAACGCATTCAATACTTCTGGTCATCTTTTAACTTTAATGATTGGACTGTGCGGCATAATGGTTGACTCATAATTGGTTTGAATTTGAGAATAAGTTCTATTTGATCTTGGTTGATCACTTATTTTCTTACCATTCTCCCTTTCTTCGAGAATATTCATCTTCGAATCTACAAGATCTCCTAGCAAAATAATTTTCTAGTCATCATCTGAATCTTGTAAAGTAAGTTTGAAGGAATCTTCTCTTCGAAACTCAAATGTGTGATTTCTTTGTGGAGGACTCCTCCCCTTTCGACATTGATCCAAAATTTCAAACTTGATCATCCCATGTGTCTTGAATGAACCACCCAAAACACTTGGAAGAAGGCGTTTCTGGGATAACAAATCCCACTCATGCACTTTTTTCACATCCACTCTTTTCTTGTTCATAATGGAAGAAAGTGTTCCTTGGATAAGCCCATTTGTAATATTGTATCCATAGTTTTTTTATGAGAAACAAAAAACGAATATAAACAAATGTATTGTATTATGAGTCTAGCAAAAACAGACAAAGTACAAGTATTTTAGTCCGTTACTTTATCTTGCAATAGTTTCTAAAAACGTTATTATAAATTTTTCCTTGTTCTTTTCTTTACCTTTCTAGGTCTGTTTTATGCTAGCGGCATTGAGTCATGGTTGGATATCTTGAGCATAGGTTGCTAATGGCTTTCATTAAAACTTTACAGGTATGACTTGCAATCAGGAATTTCTGAAACCATTGGAAAGCATGACGATATTGCAACCAATATCAGCTATTCGGATCAAACATGTGAGCTACTTTACCTCTTGACATATAACTTTTGGTAAACTATTGATCATGTTGGAAATTATAATCTTTCCTTTTAGTATGCTTGTTAATGACTGGCTAGTATTTTCCTTTTGAACTTGATGAACCGTGATTCTCATTCTCAAGAATTGGCTTATTAAATTCTGTGTTGTTTAGAAAAGTTATTTGAGACCCTTTGTCACATTGGTTATGAAAAAATAGAATTTACTCCCTTTATTTTTGTAGGCCAAATAGTAACAGCGGGTTTGGATGGAAAGATACTGTTGTGGGACACGCGTAGAGAAAAATGTGTGTCATTTGTGAGAAACAGGGATTCAGATGTGGTGTCCATGTCTATCTCTGGATTTAATTTAATTTTGGCTTCTGGAGTATGCGTATATTCACTGGACTTGCGTAACATGCAAAAACCAGTTCAACTCACAGATTCTTGTATGAAAGTTCCAGTTGCTTGTGTAAGCTCTGTTCCTTATACTGAAGGTTGGTTGACGTATTATGCAGTCTCTTCCAGATAAGGTTGACGTATTATGCAGTCTCTTCCAGATAACTTATAGCTAATATTCTGATTGTTGAAGGACGCCTTTTTTTTCTTCTTAATATGTACTTTTTGTTAAAACTATGTAGATCAGGACAACTTTTGCTTGAATTGTGTTCACTTCTAAATACTTTCCTTGCAGGATTTGCGATTGGCTCAGTAGATGGACGTGTGGCTTTGGAGATTGCTTGTCTGGACCAAGCAGATGATATCAGGTTTTTGCTTTCCCTACCTTTGAATATGGATGCTAATTACTTAAATCATACATTGAACTAGCTCGAGTTAGTATATTGCTTAAAAGCTTTATCTTATTTGTAACGGCTCAAGCTCACCGCTACCAGATATTATCCTATTTAAGCTTTTCCTTTCGGACTTTCCTTCAAGGTTTTTAAAACGCGTCTGTTAGAGAGTGGTTTTCATACCTTTCTAAAGGGTGTTTCTTTCTCCTCTCCGAATGGATGGGATCTCACATTATCCTTCTGGGGTGCTACAGTAATAATCTGTTGTCCGGTTCAGTTGATTTTAGTCAATTATGGGAACACAGAAAACTTATAACTTGTTTGGAGGTGTTTACTGGGAATTGCTTACAAAAGACTGCTTCGCTACTTTGAACACCTGTTTGAAGTGTTGTCAACGATTTTTGTTCACTGATACTATTTGCATGTTACTAGCTTGTCTAATGTCATTGGAGTATTGGTATACGTGTACTAATTCATGGTTATTATCTATACGAATGATGCACACCTTCCTTTTTAGTGCTGTAATCGTAGGAACTCTAAAGTTAAGTGTGCTTAGTTTGAAGCAATCTTATATTAGGAGATCTTTTGTGAAATTTACGAGAAAGCATGTGAGTTATGGAAGCATATTGATAGGACCCATGTGAAAATAGTCTTCATAGCTTTAGATAAATAAGGCAACATAGTCAGATTGTAGGAGAATGTTGAAGCCGTCGTGTCGTGTGTTAAAACTTGATTCTGGATTCTAAGCTTAGGGTACTCTAGTTTAAGACAAGTAAAGTGACGTAGTCAGGCTGTTAGGGAGTATTGGGATCGTCAAAGGTGAAACCTAGATTCCAAATCCCAGGCATTTCGGCCTCCTCCATCTAGTTACACTTTAGGTTTAATTAAGGAGAAAGAGACAAATGAAAAGATTCATTTTCTGCCACGTTCCATCTTTCTAGAGTGTCTATAAAAATTTAATACAATCAGAATGAAATGCTTAATCTCTCCAGAACCGTAGCTTGTCTTTAATATTCATGATATTCTTCAATCATTATTTGGGCTTGTCCAATAATTCCTGGGATGCATTATTCTTACACAGCATTTCCTTTATATTTTTGAAACAGATATATTTTCTGGTGTCACCCAAAGTCAAAGGGTAGAAGAAAGCATTTGGAGTCAGTGAATGATATTGGGTTTAATCCAATGTATGCATTATAGTTTCACCCAAAGTCAACATATTTTGTCATATTATGCTAATTTTGTATGTTGCATTTAGACACCATGGTGCCTTTGCAACGGGTGACAATGCAGGCTTTGTTAGCGTATGGGATTTCCAAAGCAAGAGAAGAATACTTGAGGTATGGGATTCTTTTGCATTGTCCTCTAAAATTTATGTGACCCTGACCTGGCCACCTATGTTCGAATGTTGTTCGTTTGGTTTTAGTTGCCTAGATTTCCAAATAGCGTGGCGTCGTTGTCGTACAACTGCGGAGGACAAGTTTTGGCTGTTGCTTCGAGTTGTACGTACCAAGAAGCTAAAGAAAGGTTCGTTTTCTGTTCGTAATCTTTGTTCGTTTCCAATCTCCATTTCCTGCATTGTGTGCTCTAAAACATTGCTTTATTTTTGGAGTTGATTTGGTGTTGTTATCTTATGTTTATGCAACAGAGAAGATCCTCCTCAAGTGTTCTTGCATGTAATAGAGGAGTACAATCCCAAATCTTTCTCTGCCTCTTCCACTGGATAG

mRNA sequence

TATTACTTCATTTCCCGCCTCTTTTGTTCGTCTAACCTCGATCCCCAGGCATGGAACCCAACAGGGTTTTGCTGGATTTTCAAAATCCGATCCAAGATGCCATCTCCACAATCCAATTCGCTCCCCGTTCCAACAATCTCCTTATTTCTTCCTGGGATTCTAGTCTTCGTTTGTACGATGTGGATAATTCTGCACTCAGACTTGAAGCTCCCTCCGAAGCTGCACTGCTTGACTGCTGCTTCCAGGACGAGTCACTGGCTTTGAGCGCTGGTTCTGATGGCTTAATAAGAAGGTATGACTTGCAATCAGGAATTTCTGAAACCATTGGAAAGCATGACGATATTGCAACCAATATCAGCTATTCGGATCAAACATGCCAAATAGTAACAGCGGGTTTGGATGGAAAGATACTGTTGTGGGACACGCGTAGAGAAAAATGTGTGTCATTTGTGAGAAACAGGGATTCAGATGTGGTGTCCATGTCTATCTCTGGATTTAATTTAATTTTGGCTTCTGGAGTATGCGTATATTCACTGGACTTGCGTAACATGCAAAAACCAGTTCAACTCACAGATTCTTGTATGAAAGTTCCAGTTGCTTGTGTAAGCTCTGTTCCTTATACTGAAGGATTTGCGATTGGCTCAGTAGATGGACGTGTGGCTTTGGAGATTGCTTGTCTGGACCAAGCAGATGATATCAGATATATTTTCTGGTGTCACCCAAAGTCAAAGGGTAGAAGAAAGCATTTGGAGTCAGTGAATGATATTGGGTTTAATCCAATACACCATGGTGCCTTTGCAACGGGTGACAATGCAGGCTTTGTTAGCGTATGGGATTTCCAAAGCAAGAGAAGAATACTTGAGTTGCCTAGATTTCCAAATAGCGTGGCGTCGTTGTCGTACAACTGCGGAGGACAAGTTTTGGCTGTTGCTTCGAGTTGTACGTACCAAGAAGCTAAAGAAAGAGAAGATCCTCCTCAAGTGTTCTTGCATGTAATAGAGGAGTACAATCCCAAATCTTTCTCTGCCTCTTCCACTGGATAG

Coding sequence (CDS)

ATGGAACCCAACAGGGTTTTGCTGGATTTTCAAAATCCGATCCAAGATGCCATCTCCACAATCCAATTCGCTCCCCGTTCCAACAATCTCCTTATTTCTTCCTGGGATTCTAGTCTTCGTTTGTACGATGTGGATAATTCTGCACTCAGACTTGAAGCTCCCTCCGAAGCTGCACTGCTTGACTGCTGCTTCCAGGACGAGTCACTGGCTTTGAGCGCTGGTTCTGATGGCTTAATAAGAAGGTATGACTTGCAATCAGGAATTTCTGAAACCATTGGAAAGCATGACGATATTGCAACCAATATCAGCTATTCGGATCAAACATGCCAAATAGTAACAGCGGGTTTGGATGGAAAGATACTGTTGTGGGACACGCGTAGAGAAAAATGTGTGTCATTTGTGAGAAACAGGGATTCAGATGTGGTGTCCATGTCTATCTCTGGATTTAATTTAATTTTGGCTTCTGGAGTATGCGTATATTCACTGGACTTGCGTAACATGCAAAAACCAGTTCAACTCACAGATTCTTGTATGAAAGTTCCAGTTGCTTGTGTAAGCTCTGTTCCTTATACTGAAGGATTTGCGATTGGCTCAGTAGATGGACGTGTGGCTTTGGAGATTGCTTGTCTGGACCAAGCAGATGATATCAGATATATTTTCTGGTGTCACCCAAAGTCAAAGGGTAGAAGAAAGCATTTGGAGTCAGTGAATGATATTGGGTTTAATCCAATACACCATGGTGCCTTTGCAACGGGTGACAATGCAGGCTTTGTTAGCGTATGGGATTTCCAAAGCAAGAGAAGAATACTTGAGTTGCCTAGATTTCCAAATAGCGTGGCGTCGTTGTCGTACAACTGCGGAGGACAAGTTTTGGCTGTTGCTTCGAGTTGTACGTACCAAGAAGCTAAAGAAAGAGAAGATCCTCCTCAAGTGTTCTTGCATGTAATAGAGGAGTACAATCCCAAATCTTTCTCTGCCTCTTCCACTGGATAG

Protein sequence

MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKVPVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAKEREDPPQVFLHVIEEYNPKSFSASSTG
BLAST of Carg26232 vs. NCBI nr
Match: XP_022951111.1 (mitotic checkpoint protein BUB3.3 [Cucurbita moschata])

HSP 1 Score: 667.2 bits (1720), Expect = 3.0e-188
Identity = 327/330 (99.09%), Postives = 328/330 (99.39%), Query Frame = 0

Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNR SDVVSMSISGFNLI+ASGVCVYSLD RNMQKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGVCVYSLDFRNMQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240
           PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG
Sbjct: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300
           FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

Query: 301 EAKEREDPPQVFLHVIEEYNPKSFSASSTG 331
           EAKEREDPPQVFLHVIEEYNPKSFSASSTG
Sbjct: 301 EAKEREDPPQVFLHVIEEYNPKSFSASSTG 330

BLAST of Carg26232 vs. NCBI nr
Match: XP_022973311.1 (mitotic checkpoint protein BUB3.3 [Cucurbita maxima])

HSP 1 Score: 656.8 bits (1693), Expect = 4.0e-185
Identity = 323/330 (97.88%), Postives = 326/330 (98.79%), Query Frame = 0

Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAP SNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPCSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNR SDVVSMSISGFNLI+ASGVCVYSLDLRN+QKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGVCVYSLDLRNIQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240
           PVACVSSVPYTEGFA+GSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG
Sbjct: 181 PVACVSSVPYTEGFAVGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300
           FNPIH GAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ
Sbjct: 241 FNPIHPGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

Query: 301 EAKEREDPPQVFLHVIEEYNPKSFSASSTG 331
           EAKEREDPPQVFLHVIEEYNPKS SASSTG
Sbjct: 301 EAKEREDPPQVFLHVIEEYNPKSSSASSTG 330

BLAST of Carg26232 vs. NCBI nr
Match: XP_023537047.1 (mitotic checkpoint protein BUB3.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 653.7 bits (1685), Expect = 3.4e-184
Identity = 319/330 (96.67%), Postives = 326/330 (98.79%), Query Frame = 0

Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNI YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNR SDVVSMSISGFNLI+ASG+CVYSLDLRN+QKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240
           PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDI 
Sbjct: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240

Query: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300
           FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQ+LAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQLLAVASSCTYQ 300

Query: 301 EAKEREDPPQVFLHVIEEYNPKSFSASSTG 331
           EAKERED PQVF+HV+EE+NPKSFSASSTG
Sbjct: 301 EAKEREDAPQVFIHVMEEFNPKSFSASSTG 330

BLAST of Carg26232 vs. NCBI nr
Match: XP_022158984.1 (mitotic checkpoint protein BUB3.3-like [Momordica charantia])

HSP 1 Score: 551.6 bits (1420), Expect = 1.8e-153
Identity = 266/320 (83.12%), Postives = 291/320 (90.94%), Query Frame = 0

Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           ME NRVLLDF    QDAISTIQFAP SNNL++SSWDSSLRLYDVD+SALRLEAPSEAALL
Sbjct: 1   MEMNRVLLDF----QDAISTIQFAPHSNNLIVSSWDSSLRLYDVDSSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120
           DCCFQDES+A SAGSDG IRRYDLQSGI +TIGKHDDIAT+I YS +TCQIV+AGLDGKI
Sbjct: 61  DCCFQDESVAFSAGSDGFIRRYDLQSGILDTIGKHDDIATHIGYSGRTCQIVSAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKV 180
           LLWDTR  K +S VRN  SDVVSMS+SGFNLI+A+GVCVYS DLR+++KP+QL DSCMKV
Sbjct: 121 LLWDTRTVKALSIVRNMGSDVVSMSLSGFNLIVATGVCVYSFDLRSLEKPIQLKDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240
           PVACVSSVPY EG A+GSVDGRVALEI+CLD+ADDIRYIFWCHPKSK RR HLESVN I 
Sbjct: 181 PVACVSSVPYCEGHAVGSVDGRVALEISCLDRADDIRYIFWCHPKSKARRNHLESVNAIA 240

Query: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300
           FNPIHHGAFATGDN GFVS+WDF SKRR+LELPRFPNSVASLSYNCGG++LAVASSCT+Q
Sbjct: 241 FNPIHHGAFATGDNEGFVSMWDFHSKRRLLELPRFPNSVASLSYNCGGELLAVASSCTFQ 300

Query: 301 EAKEREDPPQVFLHVIEEYN 321
           EAKEREDPPQVFLHV+E  N
Sbjct: 301 EAKEREDPPQVFLHVMEHNN 316

BLAST of Carg26232 vs. NCBI nr
Match: XP_011655279.1 (PREDICTED: mitotic checkpoint protein BUB3.3 [Cucumis sativus] >KGN51155.1 hypothetical protein Csa_5G468480 [Cucumis sativus])

HSP 1 Score: 528.1 bits (1359), Expect = 2.2e-146
Identity = 256/318 (80.50%), Postives = 278/318 (87.42%), Query Frame = 0

Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           ME NRVLLDFQ PIQDA+S +QFAP SNNLL+SSWDS LRLYD DN  LRLE  SEAALL
Sbjct: 1   MESNRVLLDFQIPIQDAVSRLQFAPLSNNLLVSSWDSILRLYDADNCTLRLEVASEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120
           DCCFQ+ESLALSA SDG IRRY+LQSGI ET+GKH D  T+I YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQNESLALSAASDGCIRRYELQSGIFETVGKHGDSVTHIRYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKV 180
            LWDTR +K  SFVRN  SDVVSMS+SGFNLI+ASG CVY LDLRN++K +QL DS MKV
Sbjct: 121 QLWDTRNKKSPSFVRNMGSDVVSMSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240
           PVACVSSVPY EG A+GSVDGRVAL+IACLDQ DDIRYIF CHPKSK  + HLESVNDI 
Sbjct: 181 PVACVSSVPYREGVAVGSVDGRVALDIACLDQTDDIRYIFRCHPKSKAAKNHLESVNDIV 240

Query: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300
           FNPIHHGAFATGDNAGFVS+WDFQ K RILELPRFPNSVASLSYNCGG++LAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSIWDFQRKTRILELPRFPNSVASLSYNCGGELLAVASSCTYQ 300

Query: 301 EAKEREDPPQVFLHVIEE 319
           EA ERE+PPQVFLH ++E
Sbjct: 301 EANEREEPPQVFLHNVKE 318

BLAST of Carg26232 vs. TAIR10
Match: AT1G69400.1 (Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 328.9 bits (842), Expect = 3.5e-90
Identity = 151/307 (49.19%), Postives = 226/307 (73.62%), Query Frame = 0

Query: 8   LDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDE 67
           L+F+NPI+DA+S ++F+P+SNNLL++SWDS LRLYDV++S+L LE  S+AALLDCCF++E
Sbjct: 6   LEFENPIEDAVSRLRFSPQSNNLLVASWDSYLRLYDVESSSLSLELNSQAALLDCCFENE 65

Query: 68  SLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTRR 127
           S + ++GSDG IRRYDL +G  +TIG+HDDI+T+I YS +  ++++ G D KI  WDTR+
Sbjct: 66  STSFTSGSDGFIRRYDLNAGTVDTIGRHDDISTSIVYSYEKGEVISTGFDEKIKFWDTRQ 125

Query: 128 EKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKVPVACVSS 187
            + + F  +    V  +++SG NL++     ++  DLRN+ +  Q   S ++VP+ C++S
Sbjct: 126 RESLVFSTDAGGAVGCVTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITS 185

Query: 188 VPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNPIHHG 247
           VPY+ G+A+GSVDGRVA++      + +I+Y F CHPKS+  R     +N I F+P   G
Sbjct: 186 VPYSRGYAVGSVDGRVAVDFPNTSCSSEIKYSFRCHPKSRNGRLDGVCINAIEFSPCGSG 245

Query: 248 AFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAKERED 307
            F TGDN G+V  W+ +S+RR+ ELPR+ NS+ASL+++  G++LA+ASS TYQ+AKE+E+
Sbjct: 246 TFVTGDNEGYVISWNAKSRRRLNELPRYSNSIASLAFDHTGELLAIASSHTYQDAKEKEE 305

Query: 308 PPQVFLH 315
            PQVF+H
Sbjct: 306 APQVFIH 312

BLAST of Carg26232 vs. TAIR10
Match: AT3G19590.1 (Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 222.2 bits (565), Expect = 4.6e-58
Identity = 120/320 (37.50%), Postives = 183/320 (57.19%), Query Frame = 0

Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD  +RLYDV  ++L+ E     A+LDCCF D+ 
Sbjct: 11  ELSNPPSDGISNLRFSNNSDHLLVSSWDKRVRLYDVSTNSLKGEFLHGGAVLDCCFHDDF 70

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTR-- 128
              S G+D  +RR     G  + +G HD     + YS    Q++T   D  +  WD R  
Sbjct: 71  SGFSVGADYKVRRIVFNVGKEDILGTHDKAVRCVEYSYAAGQVITGSWDKTVKCWDPRGA 130

Query: 129 --REKCVSFVRNRDSDVVSMSISGFNLILA-SGVCVYSLDLRNMQKPVQLTDSCMKVPVA 188
              E+       +   V SMS+ G  L++A +G  V   DLRNM +P Q  +S +K    
Sbjct: 131 SGPERTQVGTYLQPERVYSMSLVGHRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 190

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIGFN 248
           CV   P   G+A+ SV+GRVA+E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 191 CVRCYPNGTGYALSSVEGRVAMEFFDLSEAAQAKKYAFKCHRKSEAGRDIVYPVNSIAFH 250

Query: 249 PIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEA 308
           PI +G FATG   GFV++WD  +K+R+ +  ++P S+++LS++  GQ+LAVASS T++E 
Sbjct: 251 PI-YGTFATGGCDGFVNIWDGNNKKRLYQYSKYPTSISALSFSRDGQLLAVASSYTFEEG 310

Query: 309 KEREDPPQVFLHVIEEYNPK 323
           ++ ++P  +F+  + E   K
Sbjct: 311 EKSQEPEAIFVRSVNEIEVK 329

BLAST of Carg26232 vs. TAIR10
Match: AT1G49910.1 (Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 216.1 bits (549), Expect = 3.3e-56
Identity = 118/320 (36.88%), Postives = 181/320 (56.56%), Query Frame = 0

Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD S+RLYD +   +R E     A+LDCCF D+S
Sbjct: 10  ELSNPPSDGISNLRFSNNSDHLLVSSWDKSVRLYDANGDLMRGEFKHGGAVLDCCFHDDS 69

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTR-- 128
              S  +D  +RR D  +G  + +G H+     + YS    Q++T   D  I  WD R  
Sbjct: 70  SGFSVCADTKVRRIDFNAGKEDVLGTHEKPVRCVEYSYAAGQVITGSWDKTIKCWDPRGA 129

Query: 129 --REKCVSFVRNRDSDVVSMSISGFNLILA-SGVCVYSLDLRNMQKPVQLTDSCMKVPVA 188
              E+       +   V S+S+ G  L++A +G  V   DLRNM +P Q  +S +K    
Sbjct: 130 SGTERTQIGTYMQPERVNSLSLVGNRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 189

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIGFN 248
           CV   P   G+A+ SV+GRV++E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 190 CVRCYPNGTGYALSSVEGRVSMEFFDLSEAAQAKKYAFKCHRKSEDGRDIVYPVNAIAFH 249

Query: 249 PIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEA 308
           PI +G FA+G   GFV++WD  +K+R+ +  ++P S+A+LS++  G +LAVASS T++E 
Sbjct: 250 PI-YGTFASGGCDGFVNIWDGNNKKRLYQYSKYPTSIAALSFSRDGGLLAVASSYTFEEG 309

Query: 309 KEREDPPQVFLHVIEEYNPK 323
            +  +P  +F+  + E   K
Sbjct: 310 DKPHEPDAIFVRSVNEIEVK 328

BLAST of Carg26232 vs. TAIR10
Match: AT1G80670.1 (Transducin/WD40 repeat-like superfamily protein)

HSP 1 Score: 102.8 bits (255), Expect = 4.1e-22
Identity = 79/323 (24.46%), Postives = 140/323 (43.34%), Query Frame = 0

Query: 16  DAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAA-------LLDCCFQDES 75
           D+IS++ F+PR++ L+ +SWD+ +R +++  S   L +  +A+       L      D +
Sbjct: 26  DSISSLSFSPRADILVATSWDNQVRCWEISRSGASLASAPKASISHDQPVLCSAWKDDGT 85

Query: 76  LALSAGSDGLIRRYDLQSGISE-TIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTRR 135
              S G D   + + L SG    T+                               DTR+
Sbjct: 86  TVFSGGCDKQAKMWPLLSGGQPVTVAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDTRQ 145

Query: 136 EKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKP---VQLTDSCMKVPVAC 195
           +  V   +  D      ++S  + ++  G    +L + N+Q P    +   S +K    C
Sbjct: 146 QNPVHTQQLPDK---CYTLSVKHPLMVVGTADRNLIVFNLQNPQTEFKRIQSPLKYQTRC 205

Query: 196 VSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNPI 255
           V++ P  +GF +GS++GRV +     D      + F CH         + SVN + F+P+
Sbjct: 206 VTAFPDQQGFLVGSIEGRVGVH-HLDDSQQSKNFTFKCHRDG----NDIYSVNSLNFHPV 265

Query: 256 HHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAKE 315
            HG FAT  + G  + WD  SK+R+  + R    +   S+N  G + A A+   + +  E
Sbjct: 266 -HGTFATAGSDGAFNFWDKDSKQRLKAMSRCNQPIPCSSFNHDGSIYAYAACYDWSKGAE 325

Query: 316 REDP----PQVFLHVIEEYNPKS 324
             +P      +FLH+ +E   K+
Sbjct: 326 NHNPATAKSSIFLHLPQESEVKA 339

BLAST of Carg26232 vs. Swiss-Prot
Match: sp|F4I241|BUB33_ARATH (Mitotic checkpoint protein BUB3.3 OS=Arabidopsis thaliana OX=3702 GN=BUB3.3 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.3e-89
Identity = 151/307 (49.19%), Postives = 226/307 (73.62%), Query Frame = 0

Query: 8   LDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDE 67
           L+F+NPI+DA+S ++F+P+SNNLL++SWDS LRLYDV++S+L LE  S+AALLDCCF++E
Sbjct: 6   LEFENPIEDAVSRLRFSPQSNNLLVASWDSYLRLYDVESSSLSLELNSQAALLDCCFENE 65

Query: 68  SLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTRR 127
           S + ++GSDG IRRYDL +G  +TIG+HDDI+T+I YS +  ++++ G D KI  WDTR+
Sbjct: 66  STSFTSGSDGFIRRYDLNAGTVDTIGRHDDISTSIVYSYEKGEVISTGFDEKIKFWDTRQ 125

Query: 128 EKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKVPVACVSS 187
            + + F  +    V  +++SG NL++     ++  DLRN+ +  Q   S ++VP+ C++S
Sbjct: 126 RESLVFSTDAGGAVGCVTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITS 185

Query: 188 VPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNPIHHG 247
           VPY+ G+A+GSVDGRVA++      + +I+Y F CHPKS+  R     +N I F+P   G
Sbjct: 186 VPYSRGYAVGSVDGRVAVDFPNTSCSSEIKYSFRCHPKSRNGRLDGVCINAIEFSPCGSG 245

Query: 248 AFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAKERED 307
            F TGDN G+V  W+ +S+RR+ ELPR+ NS+ASL+++  G++LA+ASS TYQ+AKE+E+
Sbjct: 246 TFVTGDNEGYVISWNAKSRRRLNELPRYSNSIASLAFDHTGELLAIASSHTYQDAKEKEE 305

Query: 308 PPQVFLH 315
            PQVF+H
Sbjct: 306 APQVFIH 312

BLAST of Carg26232 vs. Swiss-Prot
Match: sp|Q9LJN8|BUB31_ARATH (Mitotic checkpoint protein BUB3.1 OS=Arabidopsis thaliana OX=3702 GN=BUB3.1 PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 8.3e-57
Identity = 120/320 (37.50%), Postives = 183/320 (57.19%), Query Frame = 0

Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD  +RLYDV  ++L+ E     A+LDCCF D+ 
Sbjct: 11  ELSNPPSDGISNLRFSNNSDHLLVSSWDKRVRLYDVSTNSLKGEFLHGGAVLDCCFHDDF 70

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTR-- 128
              S G+D  +RR     G  + +G HD     + YS    Q++T   D  +  WD R  
Sbjct: 71  SGFSVGADYKVRRIVFNVGKEDILGTHDKAVRCVEYSYAAGQVITGSWDKTVKCWDPRGA 130

Query: 129 --REKCVSFVRNRDSDVVSMSISGFNLILA-SGVCVYSLDLRNMQKPVQLTDSCMKVPVA 188
              E+       +   V SMS+ G  L++A +G  V   DLRNM +P Q  +S +K    
Sbjct: 131 SGPERTQVGTYLQPERVYSMSLVGHRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 190

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIGFN 248
           CV   P   G+A+ SV+GRVA+E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 191 CVRCYPNGTGYALSSVEGRVAMEFFDLSEAAQAKKYAFKCHRKSEAGRDIVYPVNSIAFH 250

Query: 249 PIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEA 308
           PI +G FATG   GFV++WD  +K+R+ +  ++P S+++LS++  GQ+LAVASS T++E 
Sbjct: 251 PI-YGTFATGGCDGFVNIWDGNNKKRLYQYSKYPTSISALSFSRDGQLLAVASSYTFEEG 310

Query: 309 KEREDPPQVFLHVIEEYNPK 323
           ++ ++P  +F+  + E   K
Sbjct: 311 EKSQEPEAIFVRSVNEIEVK 329

BLAST of Carg26232 vs. Swiss-Prot
Match: sp|Q9C701|BUB32_ARATH (Mitotic checkpoint protein BUB3.2 OS=Arabidopsis thaliana OX=3702 GN=BUB3.2 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 5.9e-55
Identity = 118/320 (36.88%), Postives = 181/320 (56.56%), Query Frame = 0

Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD S+RLYD +   +R E     A+LDCCF D+S
Sbjct: 10  ELSNPPSDGISNLRFSNNSDHLLVSSWDKSVRLYDANGDLMRGEFKHGGAVLDCCFHDDS 69

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTR-- 128
              S  +D  +RR D  +G  + +G H+     + YS    Q++T   D  I  WD R  
Sbjct: 70  SGFSVCADTKVRRIDFNAGKEDVLGTHEKPVRCVEYSYAAGQVITGSWDKTIKCWDPRGA 129

Query: 129 --REKCVSFVRNRDSDVVSMSISGFNLILA-SGVCVYSLDLRNMQKPVQLTDSCMKVPVA 188
              E+       +   V S+S+ G  L++A +G  V   DLRNM +P Q  +S +K    
Sbjct: 130 SGTERTQIGTYMQPERVNSLSLVGNRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 189

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIGFN 248
           CV   P   G+A+ SV+GRV++E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 190 CVRCYPNGTGYALSSVEGRVSMEFFDLSEAAQAKKYAFKCHRKSEDGRDIVYPVNAIAFH 249

Query: 249 PIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEA 308
           PI +G FA+G   GFV++WD  +K+R+ +  ++P S+A+LS++  G +LAVASS T++E 
Sbjct: 250 PI-YGTFASGGCDGFVNIWDGNNKKRLYQYSKYPTSIAALSFSRDGGLLAVASSYTFEEG 309

Query: 309 KEREDPPQVFLHVIEEYNPK 323
            +  +P  +F+  + E   K
Sbjct: 310 DKPHEPDAIFVRSVNEIEVK 328

BLAST of Carg26232 vs. Swiss-Prot
Match: sp|Q54DM1|BUB3_DICDI (Mitotic checkpoint protein bub3 OS=Dictyostelium discoideum OX=44689 GN=bub3 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.5e-50
Identity = 104/319 (32.60%), Postives = 174/319 (54.55%), Query Frame = 0

Query: 2   EPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLD 61
           EP     + + P  D IS++ F P S NLL++SWDS++R YD  N+  + +   +  ++D
Sbjct: 4   EPTSSFYELRLPPSDGISSVNFCPNSVNLLVTSWDSTVRCYDTQNNVQKWQYNHKGPVMD 63

Query: 62  CCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKIL 121
            CF +++   S    G ++ YD  +G+ + +G H+D   ++ Y+  T Q+ T G D ++ 
Sbjct: 64  GCFPEKNKVFSGDVFGSVKHYDPVAGVEKEVGSHEDGVRSVVYNSDTQQLFTGGWDQQLK 123

Query: 122 LWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVC---VYSLDLRNMQKPVQLTDSCM 181
           LWD R +K      +  S + +M +S  + +L  G     V   D R M+  +Q  +S +
Sbjct: 124 LWDIRSDKMEISNHDLQSKIFTMDVSPISNMLVIGTADKYVTIYDTRQMETHLQKRESSI 183

Query: 182 KVPVACVSSVPYTEGFAIGSVDGRVALEIACLDQA-DDIRYIFWCHPKSKGRRKHLESVN 241
           K    C+ +    +G+A+ SV+GR+A+E      A    +Y F CH  ++     +  VN
Sbjct: 184 KYQTRCIRTFTDGKGYALASVEGRIAMEYFDPSPAVQSKKYAFKCHRLTESGVDVVYPVN 243

Query: 242 DIGFNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSC 301
            I FNP H+G FATG     V  WD  +++R+  L  +P S++S+S+N  G +LAVASS 
Sbjct: 244 CIAFNP-HYGTFATGGCDKNVFFWDGANRKRLHALKTYPTSISSMSFNTDGNILAVASSY 303

Query: 302 TYQEAKEREDPPQVFLHVI 317
           T++E ++   P Q+F+H I
Sbjct: 304 TFEEGEKDHPPDQIFIHNI 321

BLAST of Carg26232 vs. Swiss-Prot
Match: sp|Q1JQB2|BUB3_BOVIN (Mitotic checkpoint protein BUB3 OS=Bos taurus OX=9913 GN=BUB3 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-47
Identity = 110/318 (34.59%), Postives = 179/318 (56.29%), Query Frame = 0

Query: 13  PIQDAISTIQFAPRSNN-LLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDESLAL 72
           P +D IS+++F+P ++  LL+SSWD+S+RLYDV  +++RL+     A+LDC F D + A 
Sbjct: 12  PPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAW 71

Query: 73  SAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTRREKCV 132
           S G D  ++ +DL +     +G HD     + Y  +   +VT   D  + LWD  R  C 
Sbjct: 72  SGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDP-RTPCN 131

Query: 133 SFVRNRDSDVVSMSISGFNLIL-ASGVCVYSLDLRNMQKPVQLTDSCMKVPVACVSSVPY 192
           +   ++   V ++S+SG  LI+  +G  V   DLRNM    Q  +S +K    C+ + P 
Sbjct: 132 AGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPN 191

Query: 193 TEGFAIGSVDGRVALEIACLDQADDI---RYIFWCHPKSKGRRKHLESVNDIGFNPIHHG 252
            +G+ + S++GRVA+E   LD + ++   +Y F CH   +   + +  VN I F+ IH+ 
Sbjct: 192 KQGYVLSSIEGRVAVEY--LDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHN- 251

Query: 253 AFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAKERED 312
            FATG + GFV++WD  +K+R+ +  R+P S+ASL+++  G  LA+ASS  Y+       
Sbjct: 252 TFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHP 311

Query: 313 PPQVFLHVI--EEYNPKS 324
              +F+  +   E  PKS
Sbjct: 312 EDGIFIRQVTDAETKPKS 325

BLAST of Carg26232 vs. TrEMBL
Match: tr|A0A0A0KNE2|A0A0A0KNE2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G468480 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.4e-146
Identity = 256/318 (80.50%), Postives = 278/318 (87.42%), Query Frame = 0

Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           ME NRVLLDFQ PIQDA+S +QFAP SNNLL+SSWDS LRLYD DN  LRLE  SEAALL
Sbjct: 1   MESNRVLLDFQIPIQDAVSRLQFAPLSNNLLVSSWDSILRLYDADNCTLRLEVASEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120
           DCCFQ+ESLALSA SDG IRRY+LQSGI ET+GKH D  T+I YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQNESLALSAASDGCIRRYELQSGIFETVGKHGDSVTHIRYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKV 180
            LWDTR +K  SFVRN  SDVVSMS+SGFNLI+ASG CVY LDLRN++K +QL DS MKV
Sbjct: 121 QLWDTRNKKSPSFVRNMGSDVVSMSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240
           PVACVSSVPY EG A+GSVDGRVAL+IACLDQ DDIRYIF CHPKSK  + HLESVNDI 
Sbjct: 181 PVACVSSVPYREGVAVGSVDGRVALDIACLDQTDDIRYIFRCHPKSKAAKNHLESVNDIV 240

Query: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300
           FNPIHHGAFATGDNAGFVS+WDFQ K RILELPRFPNSVASLSYNCGG++LAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSIWDFQRKTRILELPRFPNSVASLSYNCGGELLAVASSCTYQ 300

Query: 301 EAKEREDPPQVFLHVIEE 319
           EA ERE+PPQVFLH ++E
Sbjct: 301 EANEREEPPQVFLHNVKE 318

BLAST of Carg26232 vs. TrEMBL
Match: tr|A0A251R4B8|A0A251R4B8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G281000 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 4.3e-119
Identity = 210/322 (65.22%), Postives = 259/322 (80.43%), Query Frame = 0

Query: 8   LDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDE 67
           L F+NPI+DAIS I+FAP+S++LLISSWDSSLRLYDV NSALRLEA SEAALLDCCFQ+E
Sbjct: 6   LKFENPIRDAISRIRFAPKSDSLLISSWDSSLRLYDVGNSALRLEASSEAALLDCCFQNE 65

Query: 68  SLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTRR 127
           S+A SAGSDG IRRYDL SGI ++IG HDD +T + YSD+TCQ++TAGLD KIL WDTR 
Sbjct: 66  SVAFSAGSDGFIRRYDLHSGIHDSIGNHDDTSTCVGYSDETCQVITAGLDKKILSWDTRT 125

Query: 128 EKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKVPVACVSS 187
            K ++ VRN DS+V+SMS+SGF++++A G  +Y  DLR ++KP Q  DS M V + CVSS
Sbjct: 126 PKGIACVRNMDSEVMSMSLSGFDMMVAVGASIYVYDLRRLEKPFQSKDSHMGVQIVCVSS 185

Query: 188 VPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNPIHHG 247
           +PY +GFA+GSVDGRVALEI+C   +DDIRY+F CHPKS   + HL SVNDI FNPI +G
Sbjct: 186 IPYAKGFAVGSVDGRVALEISCPSNSDDIRYMFRCHPKSTDSKYHLVSVNDIVFNPIIYG 245

Query: 248 AFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAKERED 307
           AF TGDN G+V+ WD  SKRR+ +LPR+PNSVASLSY+  GQ+LAVASS TYQEA E E+
Sbjct: 246 AFVTGDNEGYVTAWDATSKRRLFQLPRYPNSVASLSYDHKGQLLAVASSYTYQEANEIEE 305

Query: 308 PPQVFLHVIEEYNPKSFSASST 330
           PP VF+H ++  N +S S  S+
Sbjct: 306 PPLVFIHEVDNDNMRSVSVGSS 327

BLAST of Carg26232 vs. TrEMBL
Match: tr|A0A2I4FXI7|A0A2I4FXI7_9ROSI (mitotic checkpoint protein BUB3.3 OS=Juglans regia OX=51240 GN=LOC109002893 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 6.7e-112
Identity = 202/326 (61.96%), Postives = 254/326 (77.91%), Query Frame = 0

Query: 4   NRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCC 63
           N   L+F+ PI+DAIS I+FAP SNNLLISSWDSSLRLYDVD S LRLEA SEAA LDCC
Sbjct: 2   NGTCLEFEKPIRDAISRIRFAPYSNNLLISSWDSSLRLYDVDGSVLRLEAQSEAAPLDCC 61

Query: 64  FQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLW 123
           FQ+ES+A SAGSDG +RRYDL  G + TIG H+DIAT+I YSD+T Q+++AGLD +I+ W
Sbjct: 62  FQNESVAFSAGSDGFVRRYDLHLGTTATIGNHNDIATSIGYSDETSQVISAGLDKEIVFW 121

Query: 124 DTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKVPVA 183
           DTR  + V+++RN  ++V SMS+SG NL++A G  +Y  DLRN+ KPVQ  DS + + + 
Sbjct: 122 DTRTAEAVAYLRNLGAEVESMSLSGSNLMVAIGASIYMYDLRNLDKPVQSEDSHVGIQIR 181

Query: 184 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNP 243
           CVSS PY++G+A+GSVDGRVA+E+      +DIRY+F CHPKSK  R HL SVNDI FNP
Sbjct: 182 CVSSSPYSKGYAVGSVDGRVAVEMCHPSNPNDIRYMFRCHPKSKDGRYHLASVNDIVFNP 241

Query: 244 IHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAK 303
           +  GAF TGDN G+V+ WD +S+RRI ELPR+ NSVASLSYN  GQ+LAVASS TYQEAK
Sbjct: 242 LICGAFVTGDNEGYVTAWDVRSRRRIFELPRYQNSVASLSYNHEGQLLAVASSYTYQEAK 301

Query: 304 EREDPPQVFLHVIEEYNPKSFSASST 330
           E E+ PQ+F+H I++   +S SA S+
Sbjct: 302 EIEELPQIFIHGIDDSYIRSVSAGSS 327

BLAST of Carg26232 vs. TrEMBL
Match: tr|A0A1S3CPL0|A0A1S3CPL0_CUCME (mitotic checkpoint protein BUB3.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503359 PE=4 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 9.0e-109
Identity = 189/233 (81.12%), Postives = 208/233 (89.27%), Query Frame = 0

Query: 82  YDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLWDTRREKCVSFVRNRDSDV 141
           Y+LQSGI ET+GKHDD  T+I YSDQTCQIVTAGLDGKI LWDTR EK +SFVRN  SDV
Sbjct: 2   YELQSGIFETVGKHDDSVTHIRYSDQTCQIVTAGLDGKIQLWDTRNEKSLSFVRNMGSDV 61

Query: 142 VSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKVPVACVSSVPYTEGFAIGSVDG 201
           +SMS+SGFNLI+ASG CVY LDLRN++K +QL DS MKVPVACVSSVPY+EG A+GSVDG
Sbjct: 62  ISMSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKVPVACVSSVPYSEGVAVGSVDG 121

Query: 202 RVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNPIHHGAFATGDNAGFVSVW 261
           RVAL+IACLDQ DDIRY F CHPKSK  + HLESVNDI FNPIHHGAFATGDNAGFVS+W
Sbjct: 122 RVALDIACLDQTDDIRYTFRCHPKSKAAKNHLESVNDIVFNPIHHGAFATGDNAGFVSIW 181

Query: 262 DFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAKEREDPPQVFLH 315
           DF+ K RILELPRFPNSVASLSYNCGG++LAVASSCTYQEA ERE+PPQVFLH
Sbjct: 182 DFRRKTRILELPRFPNSVASLSYNCGGELLAVASSCTYQEANEREEPPQVFLH 234

BLAST of Carg26232 vs. TrEMBL
Match: tr|A0A2P5A9P3|A0A2P5A9P3_9ROSA (WD repeat containing protein OS=Trema orientalis OX=63057 GN=TorRG33x02_355200 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.5e-106
Identity = 193/326 (59.20%), Postives = 247/326 (75.77%), Query Frame = 0

Query: 4   NRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCC 63
           +R  L F  PI+DAIS+IQFAP+S+NLL+SSWDS LRLYDVD+S LRLEA SEA+LLDCC
Sbjct: 2   SRAPLPFGAPIRDAISSIQFAPQSDNLLVSSWDSCLRLYDVDSSVLRLEAQSEASLLDCC 61

Query: 64  FQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKILLW 123
           F++E  A +A SDG IRRYDL SG SE IG HDD++  + YSD+TCQ+++AGLD KIL W
Sbjct: 62  FENEFTAFTAASDGSIRRYDLHSGNSEVIGNHDDLSVFVGYSDETCQVISAGLDNKILYW 121

Query: 124 DTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKVPVA 183
           DTR++K +  + N  S+V SMS+SGF++ +A G  VY  DLRN+ +P+Q   S M V + 
Sbjct: 122 DTRKKKDLLCLVNIGSEVDSMSVSGFDMTVAVGRSVYMYDLRNLNRPIQTKKSVMDVQIK 181

Query: 184 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIGFNP 243
           CVSS+P + GFA+G+VDGRVA+E++    +DDIRY+F CHPKS  +R HL SVNDI FN 
Sbjct: 182 CVSSLPNSRGFAVGAVDGRVAVELSSSSYSDDIRYVFRCHPKSTDKRFHLVSVNDIVFNS 241

Query: 244 IHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQEAK 303
           +   AF TGDN G+V  WD QS++R+ ELPR+ NSVASLS N  GQ+LAVASS TYQEA 
Sbjct: 242 LIGSAFFTGDNEGYVIAWDAQSRKRLFELPRYQNSVASLSINHRGQLLAVASSYTYQEAN 301

Query: 304 EREDPPQVFLHVIEEYNPKSFSASST 330
           E E  PQ+F+  I++ N KSFS+ S+
Sbjct: 302 EIEGTPQIFIEQIDDVNTKSFSSGSS 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951111.13.0e-18899.09mitotic checkpoint protein BUB3.3 [Cucurbita moschata][more]
XP_022973311.14.0e-18597.88mitotic checkpoint protein BUB3.3 [Cucurbita maxima][more]
XP_023537047.13.4e-18496.67mitotic checkpoint protein BUB3.3 [Cucurbita pepo subsp. pepo][more]
XP_022158984.11.8e-15383.13mitotic checkpoint protein BUB3.3-like [Momordica charantia][more]
XP_011655279.12.2e-14680.50PREDICTED: mitotic checkpoint protein BUB3.3 [Cucumis sativus] >KGN51155.1 hypot... [more]
Match NameE-valueIdentityDescription
AT1G69400.13.5e-9049.19Transducin/WD40 repeat-like superfamily protein[more]
AT3G19590.14.6e-5837.50Transducin/WD40 repeat-like superfamily protein[more]
AT1G49910.13.3e-5636.88Transducin/WD40 repeat-like superfamily protein[more]
AT1G80670.14.1e-2224.46Transducin/WD40 repeat-like superfamily protein[more]
Match NameE-valueIdentityDescription
sp|F4I241|BUB33_ARATH6.3e-8949.19Mitotic checkpoint protein BUB3.3 OS=Arabidopsis thaliana OX=3702 GN=BUB3.3 PE=2... [more]
sp|Q9LJN8|BUB31_ARATH8.3e-5737.50Mitotic checkpoint protein BUB3.1 OS=Arabidopsis thaliana OX=3702 GN=BUB3.1 PE=1... [more]
sp|Q9C701|BUB32_ARATH5.9e-5536.88Mitotic checkpoint protein BUB3.2 OS=Arabidopsis thaliana OX=3702 GN=BUB3.2 PE=2... [more]
sp|Q54DM1|BUB3_DICDI7.5e-5032.60Mitotic checkpoint protein bub3 OS=Dictyostelium discoideum OX=44689 GN=bub3 PE=... [more]
sp|Q1JQB2|BUB3_BOVIN1.6e-4734.59Mitotic checkpoint protein BUB3 OS=Bos taurus OX=9913 GN=BUB3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0KNE2|A0A0A0KNE2_CUCSA1.4e-14680.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G468480 PE=4 SV=1[more]
tr|A0A251R4B8|A0A251R4B8_PRUPE4.3e-11965.22Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G281000 PE=4 SV=1[more]
tr|A0A2I4FXI7|A0A2I4FXI7_9ROSI6.7e-11261.96mitotic checkpoint protein BUB3.3 OS=Juglans regia OX=51240 GN=LOC109002893 PE=4... [more]
tr|A0A1S3CPL0|A0A1S3CPL0_CUCME9.0e-10981.12mitotic checkpoint protein BUB3.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10350... [more]
tr|A0A2P5A9P3|A0A2P5A9P3_9ROSA2.5e-10659.20WD repeat containing protein OS=Trema orientalis OX=63057 GN=TorRG33x02_355200 P... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR036322WD40_repeat_dom_sf
IPR017986WD40_repeat_dom
IPR019775WD40_repeat_CS
IPR015943WD40/YVTN_repeat-like_dom_sf
IPR001680WD40_repeat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26232-RACarg26232-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 223..262
e-value: 2.1
score: 15.0
coord: 3..43
e-value: 0.57
score: 18.5
coord: 85..124
e-value: 0.029
score: 23.5
coord: 44..83
e-value: 21.0
score: 8.7
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 229..271
score: 8.537
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 92..133
score: 11.043
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3DG3DSA:2.130.10.10coord: 3..323
e-value: 1.0E-58
score: 201.5
NoneNo IPR availablePANTHERPTHR10971MRNA EXPORT FACTOR AND BUB3coord: 8..323
NoneNo IPR availablePANTHERPTHR10971:SF17MITOTIC CHECKPOINT PROTEIN BUB3.3coord: 8..323
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 111..125
IPR017986WD40-repeat-containing domainPROSITEPS50294WD_REPEATS_REGIONcoord: 11..133
score: 10.97
IPR017986WD40-repeat-containing domainPROSITEPS50294WD_REPEATS_REGIONcoord: 229..271
score: 8.597
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILYSSF50978WD40 repeat-likecoord: 12..296