Carg25979 (gene) Silver-seed gourd

NameCarg25979
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionHeat stress transcription factor
LocationCucurbita_argyrosperma_scaffold_311 : 68653 .. 69927 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCAGATGATTGAAGGTGATAAGCCAAATCCAGCGCCATTTTTGACCAAAACCTATGATTTGGTGGATGATCCTCGCACTGATCATGTTGTTTCTTGGGGCCAATCTCATTCCACCTTTGTTGTTTGGAGCCCTCCTGAGTTTGCTACTCATGTTCTTCCCAACTACTTCAAACACAACAATTTCTCTAGCTTTGTTCGTCAACTCAACACTTACGTACGTATCTGCCTCGTTCTCTTGTTCTTGTTCTCGTTCTTGTTCTTGTTCTCTGTCTTTGAGAGAGTTTGAACTTATGGTTTATGGAGAAGGATAGTTCTCGTGTATAATTAACCATCTGGGTCTAGGCCGACTTCCTCTCATGGGATTCTTTAGCCTAAAGGGATGCATGAGATGTTTTCAATCTAGATAGAATTTAAACCTCGGGTCCATGAATGAGACGTCAGTGTCCAATCATTACGAACTATGCTTAGATTAGCTTTTTTTTTTTATCGGATTTGAACCTACAAGAGACCGTAGCATTTAATCGTCATCTAACTAGCTAGAGAGATTCGTTATGAGATTCAAACTTACTAACGCTCGAGAGCAGATATCAATGTCCAAACAGTTGAGTTTATGCTTGGTATAGCGTTATATATTGTTTGACGTTTTTTTCAATCCTTAGAACAAATGAAGCCAAAAACGATTTGATTGACGTTTTTTTCAAAGTAGAATTCAAACCCATGATTCGTTAATGTTAATCGATTACGCTATGCTCGAGTTAGTGAATAATTTGATTGTGATTGACAGGGATTCAAGAAAGTAGTAGCAGAGAGATGGGAATTTGGGAACGAAAACTTCAAGAAAGGAGAGAAACAATTACTCTCACAGATTCACAGAAGAAAATCTCATAATTACAATAATAATAATAATAACAATCTCCAATTCTTCCATGTACACGGCGAGCTCTTCACCGCCACTTCCTCCTCCGATTCCGACGTGCTGGCGGCGCTGATGCAAGACAACCGGCGTCTACGACGGAGGAACTTCATGCTGCTATCGGAGCTGACCCAGATGAAGAATCTCTACTCGGACATCATATACTTCATCCAAAACCACGTGAAGCCATTTGATCAGAGAAACGGGAAATGGGTGGCGAAGTTGGTGGAGTTGGAGGCTCCGCCGCCGCAAGTAGCGGTGGCTAAGGCGGCGGGGGAATCAGAAGAGGTGAAGCTATTTGGGGTTCCGATTAGAGGGAAGAAGAGAGGAGCTTCAGATGAAGAGGATAGAGTTTGA

mRNA sequence

ATGGAGCAGATGATTGAAGGTGATAAGCCAAATCCAGCGCCATTTTTGACCAAAACCTATGATTTGGTGGATGATCCTCGCACTGATCATGTTGTTTCTTGGGGCCAATCTCATTCCACCTTTGTTGTTTGGAGCCCTCCTGAGTTTGCTACTCATGTTCTTCCCAACTACTTCAAACACAACAATTTCTCTAGCTTTGTTCGTCAACTCAACACTTACGGATTCAAGAAAGTAGTAGCAGAGAGATGGGAATTTGGGAACGAAAACTTCAAGAAAGGAGAGAAACAATTACTCTCACAGATTCACAGAAGAAAATCTCATAATTACAATAATAATAATAATAACAATCTCCAATTCTTCCATGTACACGGCGAGCTCTTCACCGCCACTTCCTCCTCCGATTCCGACGTGCTGGCGGCGCTGATGCAAGACAACCGGCGTCTACGACGGAGGAACTTCATGCTGCTATCGGAGCTGACCCAGATGAAGAATCTCTACTCGGACATCATATACTTCATCCAAAACCACGTGAAGCCATTTGATCAGAGAAACGGGAAATGGGTGGCGAAGTTGGTGGAGTTGGAGGCTCCGCCGCCGCAAGTAGCGGTGGCTAAGGCGGCGGGGGAATCAGAAGAGGTGAAGCTATTTGGGGTTCCGATTAGAGGGAAGAAGAGAGGAGCTTCAGATGAAGAGGATAGAGTTTGA

Coding sequence (CDS)

ATGGAGCAGATGATTGAAGGTGATAAGCCAAATCCAGCGCCATTTTTGACCAAAACCTATGATTTGGTGGATGATCCTCGCACTGATCATGTTGTTTCTTGGGGCCAATCTCATTCCACCTTTGTTGTTTGGAGCCCTCCTGAGTTTGCTACTCATGTTCTTCCCAACTACTTCAAACACAACAATTTCTCTAGCTTTGTTCGTCAACTCAACACTTACGGATTCAAGAAAGTAGTAGCAGAGAGATGGGAATTTGGGAACGAAAACTTCAAGAAAGGAGAGAAACAATTACTCTCACAGATTCACAGAAGAAAATCTCATAATTACAATAATAATAATAATAACAATCTCCAATTCTTCCATGTACACGGCGAGCTCTTCACCGCCACTTCCTCCTCCGATTCCGACGTGCTGGCGGCGCTGATGCAAGACAACCGGCGTCTACGACGGAGGAACTTCATGCTGCTATCGGAGCTGACCCAGATGAAGAATCTCTACTCGGACATCATATACTTCATCCAAAACCACGTGAAGCCATTTGATCAGAGAAACGGGAAATGGGTGGCGAAGTTGGTGGAGTTGGAGGCTCCGCCGCCGCAAGTAGCGGTGGCTAAGGCGGCGGGGGAATCAGAAGAGGTGAAGCTATTTGGGGTTCCGATTAGAGGGAAGAAGAGAGGAGCTTCAGATGAAGAGGATAGAGTTTGA

Protein sequence

MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRRKSHNYNNNNNNNLQFFHVHGELFTATSSSDSDVLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVKPFDQRNGKWVAKLVELEAPPPQVAVAKAAGESEEVKLFGVPIRGKKRGASDEEDRV
BLAST of Carg25979 vs. NCBI nr
Match: XP_022925361.1 (heat stress transcription factor B-4b-like [Cucurbita moschata])

HSP 1 Score: 392.1 bits (1006), Expect = 1.3e-105
Identity = 230/234 (98.29%), Postives = 231/234 (98.72%), Query Frame = 0

Query: 1   MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH 60
           MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPE ATHVLPNYFKH
Sbjct: 1   MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPELATHVLPNYFKH 60

Query: 61  NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRRXXXXXXXXXXXXXXXX 120
           NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRRXXXXXXXXXXXXXXXX
Sbjct: 61  NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRRXXXXXXXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVKPF 180
           XXXXXXXXXXXXXXXX LAAL QDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVKPF
Sbjct: 121 XXXXXXXXXXXXXXXXVLAALTQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVKPF 180

Query: 181 DQRNGKWVAKLVELEAPPPQVAVAKAAGESEEVKLFGVPIRGKKRGASDEEDRV 235
           DQRNGKWVAKLVELEAPPPQVAVA+AAGESEEVKLFGVPIRGKKRGASDEEDRV
Sbjct: 181 DQRNGKWVAKLVELEAPPPQVAVAEAAGESEEVKLFGVPIRGKKRGASDEEDRV 234

BLAST of Carg25979 vs. NCBI nr
Match: XP_023531680.1 (heat stress transcription factor B-4b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.4 bits (999), Expect = 8.5e-105
Identity = 224/236 (94.92%), Postives = 225/236 (95.34%), Query Frame = 0

Query: 1   MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH 60
           MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH
Sbjct: 1   MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH 60

Query: 61  NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR--XXXXXXXXXXXXXX 120
           NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR  XXXXXXXXXXXXXX
Sbjct: 61  NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRRKXXXXXXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVK 180
           XXXXXX     XXXXX   LAAL QDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVK
Sbjct: 121 XXXXXXELFTAXXXXXSDVLAALTQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVK 180

Query: 181 PFDQRNGKWVAKLVELEAPPPQVAVAKAAGESEEVKLFGVPIRGKKRGASDEEDRV 235
           PFDQRNGKWVAKLVELEAPPPQVAVA+AAGESEEVKLFGVPIRGKKRGASDEEDRV
Sbjct: 181 PFDQRNGKWVAKLVELEAPPPQVAVAEAAGESEEVKLFGVPIRGKKRGASDEEDRV 236

BLAST of Carg25979 vs. NCBI nr
Match: XP_022974122.1 (heat stress transcription factor B-4b-like [Cucurbita maxima])

HSP 1 Score: 384.0 bits (985), Expect = 3.6e-103
Identity = 217/236 (91.95%), Postives = 218/236 (92.37%), Query Frame = 0

Query: 1   MEQMIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH 60
           MEQ IEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH
Sbjct: 1   MEQKIEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH 60

Query: 61  NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR--XXXXXXXXXXXXXX 120
           NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR  XXXXXXXXXXXXXX
Sbjct: 61  NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRRXXXXXXXXXXXXXXXX 120

Query: 121 XXXXXXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVK 180
           XXXXXX             LAAL QDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVK
Sbjct: 121 XXXXXXELFTATSSSDSDMLAALTQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVK 180

Query: 181 PFDQRNGKWVAKLVELEAPPPQVAVAKAAGESEEVKLFGVPIRGKKRGASDEEDRV 235
            FDQRNGKWVAKLVELEAPPPQVAVA+AAGESEEVKLFGVPIRGKKRGASDEEDRV
Sbjct: 181 AFDQRNGKWVAKLVELEAPPPQVAVAEAAGESEEVKLFGVPIRGKKRGASDEEDRV 236

BLAST of Carg25979 vs. NCBI nr
Match: XP_022153268.1 (heat stress transcription factor B-4b-like [Momordica charantia])

HSP 1 Score: 270.8 bits (691), Expect = 4.4e-69
Identity = 150/251 (59.76%), Postives = 165/251 (65.74%), Query Frame = 0

Query: 2   EQMIEGD-KPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKH 61
           E +IE D K NPAPFLTKTYDLV+DPRTDH+VSWGQSH+TFVVW PPEFA+HVLPNYFKH
Sbjct: 3   ENIIECDQKGNPAPFLTKTYDLVEDPRTDHIVSWGQSHTTFVVWRPPEFASHVLPNYFKH 62

Query: 62  NNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR---------------- 121
           NNFSSFVRQLNTYGFKK+VA+RWEFGNENFKKGEK+LLS+IHRR                
Sbjct: 63  NNFSSFVRQLNTYGFKKIVADRWEFGNENFKKGEKKLLSEIHRRKSHXXXXXXXXXXXXX 122

Query: 122 --XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQM 181
             X                                L AL QDNRRLRRRN+MLLSEL QM
Sbjct: 123 XXXHQNPLFHQFFHLDHEPLTLYYTAATAAADSDILEALTQDNRRLRRRNYMLLSELAQM 182

Query: 182 KNLYSDIIYFIQNHVKPFDQRNGK--WVAKLVELEAPPPQVAVAKAAGESE-EVKLFGVP 231
           KNLYSDIIYFIQNHV P   +  +   V KLVEL++P          G     VKLFGVP
Sbjct: 183 KNLYSDIIYFIQNHVSPLQHKTARSSSVPKLVELDSPXXXXXXXXXXGNGNGTVKLFGVP 242

BLAST of Carg25979 vs. NCBI nr
Match: XP_022988196.1 (heat stress transcription factor B-4b-like [Cucurbita maxima])

HSP 1 Score: 265.4 bits (677), Expect = 1.8e-67
Identity = 173/231 (74.89%), Postives = 191/231 (82.68%), Query Frame = 0

Query: 6   EGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSS 65
           E  K NPAPFL KTY+LV+D  TDH+VSWG+SH+TFVVW PPEFATH+LPNYFKHNNFSS
Sbjct: 16  ESQKANPAPFLAKTYELVEDAATDHIVSWGRSHTTFVVWRPPEFATHILPNYFKHNNFSS 75

Query: 66  FVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIH---RRXXXXXXXXXXXXXXXXXX 125
           FVRQLN+YGFKK+VA+RWEFGN+NFKKGEKQLL +I      XXXXXXXXXXXXXXXXXX
Sbjct: 76  FVRQLNSYGFKKIVAKRWEFGNQNFKKGEKQLLLEIQXXXXXXXXXXXXXXXXXXXXXXX 135

Query: 126 XXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVKPFDQ 185
           XXXXXXXXXXXXXXXLAAL QDNRRLRRRN+MLLSELTQMKNLYSDIIYFIQNHVKP D 
Sbjct: 136 XXXXXXXXXXXXXXXLAALTQDNRRLRRRNYMLLSELTQMKNLYSDIIYFIQNHVKPLDH 195

Query: 186 RNGKWVAKLVELEAPPPQVAVAKAAGESE---EVKLFGVPIRGKKRGASDE 231
           + G+ V KLV+L+           AG  E    V+LFGVPIRGKKR   +E
Sbjct: 196 KYGRSVPKLVDLKXXXXXXXXXXXAGVEEGSGSVQLFGVPIRGKKRVHHEE 246

BLAST of Carg25979 vs. TAIR10
Match: AT1G46264.1 (heat shock transcription factor B4)

HSP 1 Score: 193.7 bits (491), Expect = 1.2e-49
Identity = 123/215 (57.21%), Postives = 139/215 (64.65%), Query Frame = 0

Query: 1   MEQMIEGD-KPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFK 60
           ++ M+EG  K  PAPFLTKTY LVDDP TDHVVSWG   +TFVVW PPEFA  +LPNYFK
Sbjct: 20  IQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFK 79

Query: 61  HNNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR--------------- 120
           HNNFSSFVRQLNTYGF+K+V +RWEF NE FK+GEK LL +IHRR               
Sbjct: 80  HNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMS 139

Query: 121 --------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLAALMQD 180
                               XXXXXXXXXXXXXXXXXX               + AL +D
Sbjct: 140 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRPRVIPQQIDTAAQVTALSED 199

BLAST of Carg25979 vs. TAIR10
Match: AT5G62020.1 (heat shock transcription factor B2A)

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-34
Identity = 126/262 (48.09%), Postives = 163/262 (62.21%), Query Frame = 0

Query: 1   MEQMIEGDKPN----PAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPN 60
           ++ MI G+  +    P PFLTKT++LV+D   D V+SW +  S+F+VW+P +FA  +LP 
Sbjct: 6   VDAMITGESSSQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPK 65

Query: 61  YFKHNNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRRXXXXXXXXXXXX 120
           +FKHNNFSSFVRQLNTYGFKKVV +RWEF N+ FK+GEK+LL +I   XXXXXXXXXXXX
Sbjct: 66  HFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQXXXXXXXXXXXXXX 125

Query: 121 XXXXXXXXXXXXXXXXXXXXXL-------------------------AALMQDNRRLRRR 180
           XXXXXXXXXXXXXXXXXXXXX                            L+++N +LR +
Sbjct: 126 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYCHQTKTTGNGGLSVELLEENEKLRSQ 185

Query: 181 NFMLLSELTQMKNLYSDIIYFIQNHV--KPFDQRNGKWVAKLVELEAPPPQVAVAKAAGE 230
           N  L  ELTQMK++  +I   + N+V  +P D+      +    +E  P +        E
Sbjct: 186 NIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKRFSEMEIEE 245

BLAST of Carg25979 vs. TAIR10
Match: AT4G17750.1 (heat shock factor 1)

HSP 1 Score: 141.4 bits (355), Expect = 7.3e-34
Identity = 60/93 (64.52%), Postives = 76/93 (81.72%), Query Frame = 0

Query: 12  PAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSSFVRQLN 71
           P PFL+KTYD+V+DP TD +VSW  ++++F+VW PPEF+  +LP YFKHNNFSSFVRQLN
Sbjct: 50  PPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLN 109

Query: 72  TYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR 105
           TYGF+KV  +RWEF NE F +G+K LL +I RR
Sbjct: 110 TYGFRKVDPDRWEFANEGFLRGQKHLLKKISRR 142

BLAST of Carg25979 vs. TAIR10
Match: AT3G22830.1 (heat shock transcription factor A6B)

HSP 1 Score: 139.8 bits (351), Expect = 2.1e-33
Identity = 63/102 (61.76%), Postives = 79/102 (77.45%), Query Frame = 0

Query: 3   QMIEG-DKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHN 62
           Q +EG  +  P PFLTKTYDLV+D RT+HVVSW +S+++F+VW P  F+  +LP +FKHN
Sbjct: 49  QPLEGLHESGPPPFLTKTYDLVEDSRTNHVVSWSKSNNSFIVWDPQAFSVTLLPRFFKHN 108

Query: 63  NFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHR 104
           NFSSFVRQLNTYGF+KV  +RWEF NE F +G+K LL  I R
Sbjct: 109 NFSSFVRQLNTYGFRKVNPDRWEFANEGFLRGQKHLLKNIRR 150

BLAST of Carg25979 vs. TAIR10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein)

HSP 1 Score: 139.4 bits (350), Expect = 2.8e-33
Identity = 62/93 (66.67%), Postives = 73/93 (78.49%), Query Frame = 0

Query: 12  PAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSSFVRQLN 71
           P PFLTKTY LV+DP  D ++SW +  +TF+VW P EFA  +LP YFKHNNFSSFVRQLN
Sbjct: 57  PTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSSFVRQLN 116

Query: 72  TYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR 105
           TYGF+KVV +RWEF N+ FK+GEK LL  I RR
Sbjct: 117 TYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRR 149

BLAST of Carg25979 vs. Swiss-Prot
Match: sp|Q9C635|HSFB4_ARATH (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.2e-48
Identity = 123/215 (57.21%), Postives = 139/215 (64.65%), Query Frame = 0

Query: 1   MEQMIEGD-KPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFK 60
           ++ M+EG  K  PAPFLTKTY LVDDP TDHVVSWG   +TFVVW PPEFA  +LPNYFK
Sbjct: 20  IQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFK 79

Query: 61  HNNFSSFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR--------------- 120
           HNNFSSFVRQLNTYGF+K+V +RWEF NE FK+GEK LL +IHRR               
Sbjct: 80  HNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMS 139

Query: 121 --------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLAALMQD 180
                               XXXXXXXXXXXXXXXXXX               + AL +D
Sbjct: 140 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSRPRVIPQQIDTAAQVTALSED 199

BLAST of Carg25979 vs. Swiss-Prot
Match: sp|Q67U94|HFB4C_ORYSJ (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.8e-43
Identity = 118/232 (50.86%), Postives = 130/232 (56.03%), Query Frame = 0

Query: 9   KPNPAPFLTKTYDLVDDPRTDHVVSWGQSH-STFVVWSPPEFATHVLPNYFKHNNFSSFV 68
           K  PAPFLTKTY LVDDP TDH+VSWG    STFVVW PPEFA  +LPNYFKHNNFSSFV
Sbjct: 19  KAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILPNYFKHNNFSSFV 78

Query: 69  RQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHR------------------------ 128
           RQLNTYGF+KVV ERWEF NE F+KGEKQLL++IH                         
Sbjct: 79  RQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHXXXXXXXXXXXXXXXXXXXXXXXXX 138

Query: 129 ------------------------------------RXXXXXXXXXXXXXXXXXXXXXXX 180
                                               R                 XXXXXX
Sbjct: 139 XXXXXXXXXXXXXXXXXDDGVVAAHGIGMPFPQPHWREPNLPVATRLLALGGPAXXXXXX 198

BLAST of Carg25979 vs. Swiss-Prot
Match: sp|Q6Z9R8|HFB4A_ORYSJ (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-41
Identity = 102/225 (45.33%), Postives = 115/225 (51.11%), Query Frame = 0

Query: 12  PAPFLTKTYDLVDDPRTDHVVSW-----GQSHSTFVVWSPPEFATHVLPNYFKHNNFSSF 71
           PAPFLTKTY LVDDP TDHVVSW     G+S S+FVVW PPEFA  +LPNYFKH+NFSSF
Sbjct: 23  PAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHSNFSSF 82

Query: 72  VRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIH------------------------ 131
           VRQLNTYGF+KVV ERWEF NE F+KGEKQLL +IH                        
Sbjct: 83  VRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHXXXXXXXXXXXXXXXXXXXXXXXX 142

Query: 132 ----------------RRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLA------- 180
                           R                                  +A       
Sbjct: 143 FAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPARRAAA 202

BLAST of Carg25979 vs. Swiss-Prot
Match: sp|Q7XHZ0|HFB4B_ORYSJ (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.5e-39
Identity = 72/94 (76.60%), Postives = 82/94 (87.23%), Query Frame = 0

Query: 9   KPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSSFVR 68
           KP PAPFLTKTY LVDDP TDH+VSWG+  +TFVVW PPEFA  +LPNYFKHNNFSSFVR
Sbjct: 29  KPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 69  QLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIH 103
           QLNTYGF+K+VA+RWEF NE F+KG K LL++IH
Sbjct: 89  QLNTYGFRKIVADRWEFANEFFRKGAKHLLAEIH 122

BLAST of Carg25979 vs. Swiss-Prot
Match: sp|Q10KX8|HFB4D_ORYSJ (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 9.4e-39
Identity = 72/93 (77.42%), Postives = 81/93 (87.10%), Query Frame = 0

Query: 12  PAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSSFVRQLN 71
           PAPFL+KTY LVDDP TD VVSWG+  +TFVVW PPEFA  +LPNYFKHNNFSSFVRQLN
Sbjct: 34  PAPFLSKTYQLVDDPSTDDVVSWGEDEATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLN 93

Query: 72  TYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR 105
           TYGF+K+VA+RWEF NE F+KG K LLS+IHRR
Sbjct: 94  TYGFRKIVADRWEFANEFFRKGAKHLLSEIHRR 126

BLAST of Carg25979 vs. TrEMBL
Match: tr|A0A0A0M2Y7|A0A0A0M2Y7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629180 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.5e-65
Identity = 173/235 (73.62%), Postives = 191/235 (81.28%), Query Frame = 0

Query: 11  NPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSSFVRQL 70
           NPAPFL+KTYDLV+DP TDH+VSWGQS +TF+VW P EFATH+LPNYFKHNNFSSFVRQL
Sbjct: 25  NPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIVWRPSEFATHILPNYFKHNNFSSFVRQL 84

Query: 71  NTYGFKKVVAERWEFGNENFKKGEKQLLSQI------HRRXXXXXXXXXXXXXXXXXXXX 130
           NTYGFKK+VAERWEFGNENFKKGEKQLL +I         XXXXXXXXXXXXXXXXXXXX
Sbjct: 85  NTYGFKKIVAERWEFGNENFKKGEKQLLLEIXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 144

Query: 131 XXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVKPFDQRN 190
           XXXXXXXXXXXX LAAL QDNRRLRRRNFMLLSEL QMKNLYSDIIYFIQN+VKP D + 
Sbjct: 145 XXXXXXXXXXXXILAALTQDNRRLRRRNFMLLSELAQMKNLYSDIIYFIQNNVKPLDNKY 204

Query: 191 -GKWVAKLVELEAP------PPQVAVAKAAGESEEVKLFGVPIRGKKRGASDEED 233
             + V KLVEL+ P       P++ + +  G    VKLFGVPIRGKKR   +E +
Sbjct: 205 CNRSVPKLVELDPPSSPTPMSPEIRLEEGNG---MVKLFGVPIRGKKRVLPEESE 256

BLAST of Carg25979 vs. TrEMBL
Match: tr|A0A1S3BPZ9|A0A1S3BPZ9_CUCME (heat stress transcription factor B-4b-like OS=Cucumis melo OX=3656 GN=LOC103491968 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 7.4e-65
Identity = 170/235 (72.34%), Postives = 190/235 (80.85%), Query Frame = 0

Query: 11  NPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFSSFVRQL 70
           NPAPFL+KTYDLV+DP TDH+VSWGQS +TF+VW P EFATH+LPNYFKHNNFSSFVRQL
Sbjct: 25  NPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIVWRPSEFATHILPNYFKHNNFSSFVRQL 84

Query: 71  NTYGFKKVVAERWEFGNENFKKGEKQLLSQ------IHRRXXXXXXXXXXXXXXXXXXXX 130
           NTYGFKK+VAERWEFGNENFKKGEKQLL +          XXXXXXXXXXXXXXXXXXXX
Sbjct: 85  NTYGFKKIVAERWEFGNENFKKGEKQLLLEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 144

Query: 131 XXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHVKPFDQRN 190
           XXXXXXXXXXX  L AL QDNRRLRRRNFMLLSEL QMKNLYSDIIYFIQN+VKP D + 
Sbjct: 145 XXXXXXXXXXXDILTALTQDNRRLRRRNFMLLSELAQMKNLYSDIIYFIQNNVKPLDNKY 204

Query: 191 -GKWVAKLVELEAP------PPQVAVAKAAGESEEVKLFGVPIRGKKRGASDEED 233
             + V KLVEL++P       P++ + + +G    VKLFGVPIRGKKR   +E +
Sbjct: 205 CNRSVPKLVELDSPSSPMPMSPEIRLEEGSG---MVKLFGVPIRGKKRVLPEESE 256

BLAST of Carg25979 vs. TrEMBL
Match: tr|G7KCV7|G7KCV7_MEDTR (Heat shock transcription factor OS=Medicago truncatula OX=3880 GN=11432338 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.6e-59
Identity = 131/233 (56.22%), Postives = 150/233 (64.38%), Query Frame = 0

Query: 5   IEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFS 64
           +E  K  PAPFLTKTY LVDDP TDH+VSW    +TFVVW PPEFA  +LPN+FKHNNFS
Sbjct: 16  MESQKSVPAPFLTKTYQLVDDPLTDHIVSWSDDETTFVVWRPPEFARDLLPNFFKHNNFS 75

Query: 65  SFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR-------XXXXXXXXXXXXX 124
           SFVRQLNTYGFKKVVA+RWEF N+ FKKG K LL +IHRR                    
Sbjct: 76  SFVRQLNTYGFKKVVADRWEFANDYFKKGAKHLLCEIHRRKTPQHYQQQYYEQSPQIFQP 135

Query: 125 XXXXXXXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQNHV 184
                               L AL +DN+RLRR+NFMLLSEL+ MKNLY+DIIYFIQNHV
Sbjct: 136 DESICWIDSPLPSPKSNTDILTALSEDNQRLRRKNFMLLSELSHMKNLYNDIIYFIQNHV 195

Query: 185 ---KPFDQR--NGKWVAKLVELEAPPPQVAVAKAAGESEEVKLFGVPIRGKKR 226
               PF+QR  N   + KLVEL++  PQ+   K    S  VKLFGVP+ GKKR
Sbjct: 196 SPASPFEQRSNNSATILKLVELDSSSPQLPNDKDC-NSSSVKLFGVPLCGKKR 247

BLAST of Carg25979 vs. TrEMBL
Match: tr|A0A151U1R7|A0A151U1R7_CAJCA (Heat stress transcription factor B-4b OS=Cajanus cajan OX=3821 GN=KK1_005852 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 3.0e-58
Identity = 135/250 (54.00%), Postives = 153/250 (61.20%), Query Frame = 0

Query: 5   IEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFS 64
           +E  K  PAPFLTKTY LVDDP TDH+VSWG   +TFVV  PPEFA  +LPNYFKHNNFS
Sbjct: 16  MESQKSVPAPFLTKTYQLVDDPCTDHIVSWGDDETTFVVRRPPEFARDLLPNYFKHNNFS 75

Query: 65  SFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR-----------XXXXXXXXX 124
           SFVRQLNTYGFKKV A+RWEF NE F+KG K LL +IHRR                    
Sbjct: 76  SFVRQLNTYGFKKVAADRWEFANEYFRKGAKHLLCEIHRRKTPHHYQQHYHMHDHPPSQL 135

Query: 125 XXXXXXXXXXXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFI 184
                                   L AL +DN+RLRR+NFMLLSELT MKNLY+DIIYFI
Sbjct: 136 LQPEENLCWIDAPPLPSPKPGTDILTALSEDNQRLRRKNFMLLSELTHMKNLYNDIIYFI 195

Query: 185 QNHVKP--FDQRNGKWVAKLVELEAPP--PQVAVAKAAG----------ESEE----VKL 226
           QNHV P  ++QR+   + KLVEL++ P  P V V  A G           SEE    VKL
Sbjct: 196 QNHVTPASYEQRSSSAILKLVELDSSPQSPNVVVRPAKGHVMEKSLMISSSEEANSSVKL 255

BLAST of Carg25979 vs. TrEMBL
Match: tr|A0A2K3LMR4|A0A2K3LMR4_TRIPR (Heat stress transcription factor b-4-like protein OS=Trifolium pratense OX=57577 GN=L195_g018776 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.9e-58
Identity = 132/239 (55.23%), Postives = 151/239 (63.18%), Query Frame = 0

Query: 5   IEGDKPNPAPFLTKTYDLVDDPRTDHVVSWGQSHSTFVVWSPPEFATHVLPNYFKHNNFS 64
           +E  K  PAPFLTKTY LVDDP TDH+VSW    +TFVVW PPEFA  +LPN+FKHNNFS
Sbjct: 5   MESQKSVPAPFLTKTYQLVDDPLTDHIVSWSDDETTFVVWRPPEFARDLLPNFFKHNNFS 64

Query: 65  SFVRQLNTYGFKKVVAERWEFGNENFKKGEKQLLSQIHRR---------XXXXXXXXXXX 124
           SFVRQLNTYGFKKVVA+RWEF NE FKKG K LL +IHRR                    
Sbjct: 65  SFVRQLNTYGFKKVVADRWEFANEYFKKGAKHLLCEIHRRKTPQNYHQQYYEQQSPQFHQ 124

Query: 125 XXXXXXXXXXXXXXXXXXXXXXLAALMQDNRRLRRRNFMLLSELTQMKNLYSDIIYFIQN 184
                                 L AL +DN+RLR++NFMLLSELT MKNLY+DIIYFIQN
Sbjct: 125 QDHESICWIDSPLPSPKSNTDILTALSEDNQRLRKKNFMLLSELTHMKNLYNDIIYFIQN 184

Query: 185 HVKP----FDQR-NGKWVAKLVELEAPPPQVA----VAKAAGESEEVKLFGVPIRGKKR 226
           HV P    F+QR N   + KLVEL++   Q+     V K +  S  VKLFGVP+ GKKR
Sbjct: 185 HVSPATTSFEQRSNNSAILKLVELDSSASQLGNDNNVVKDS-NSSYVKLFGVPLCGKKR 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022925361.11.3e-10598.29heat stress transcription factor B-4b-like [Cucurbita moschata][more]
XP_023531680.18.5e-10594.92heat stress transcription factor B-4b-like [Cucurbita pepo subsp. pepo][more]
XP_022974122.13.6e-10391.95heat stress transcription factor B-4b-like [Cucurbita maxima][more]
XP_022153268.14.4e-6959.76heat stress transcription factor B-4b-like [Momordica charantia][more]
XP_022988196.11.8e-6774.89heat stress transcription factor B-4b-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G46264.11.2e-4957.21heat shock transcription factor B4[more]
AT5G62020.12.5e-3448.09heat shock transcription factor B2A[more]
AT4G17750.17.3e-3464.52heat shock factor 1[more]
AT3G22830.12.1e-3361.76heat shock transcription factor A6B[more]
AT4G11660.12.8e-3366.67winged-helix DNA-binding transcription factor family protein[more]
Match NameE-valueIdentityDescription
sp|Q9C635|HSFB4_ARATH2.2e-4857.21Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
sp|Q67U94|HFB4C_ORYSJ4.8e-4350.86Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
sp|Q6Z9R8|HFB4A_ORYSJ1.6e-4145.33Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
sp|Q7XHZ0|HFB4B_ORYSJ2.5e-3976.60Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
sp|Q10KX8|HFB4D_ORYSJ9.4e-3977.42Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0M2Y7|A0A0A0M2Y7_CUCSA2.5e-6573.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G629180 PE=3 SV=1[more]
tr|A0A1S3BPZ9|A0A1S3BPZ9_CUCME7.4e-6572.34heat stress transcription factor B-4b-like OS=Cucumis melo OX=3656 GN=LOC1034919... [more]
tr|G7KCV7|G7KCV7_MEDTR1.6e-5956.22Heat shock transcription factor OS=Medicago truncatula OX=3880 GN=11432338 PE=3 ... [more]
tr|A0A151U1R7|A0A151U1R7_CAJCA3.0e-5854.00Heat stress transcription factor B-4b OS=Cajanus cajan OX=3821 GN=KK1_005852 PE=... [more]
tr|A0A2K3LMR4|A0A2K3LMR4_TRIPR3.9e-5855.23Heat stress transcription factor b-4-like protein OS=Trifolium pratense OX=57577... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: Molecular Function
TermDefinition
GO:0043565sequence-specific DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: INTERPRO
TermDefinition
IPR036390WH_DNA-bd_sf
IPR027725HSF_fam
IPR036388WH-like_DNA-bd_sf
IPR000232HSF_DNA-bd
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25979-RACarg25979-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 53..65
score: 76.04
coord: 66..78
score: 81.54
coord: 15..38
score: 45.95
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 11..104
e-value: 9.8E-54
score: 194.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 15..104
e-value: 1.4E-29
score: 102.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 54..78
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3DG3DSA:1.10.10.10coord: 8..106
e-value: 3.9E-37
score: 128.8
NoneNo IPR availablePANTHERPTHR10015:SF177SUBFAMILY NOT NAMEDcoord: 4..223
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 4..223
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 11..104