Carg25859 (gene) Silver-seed gourd

NameCarg25859
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionEndoglucanase
LocationCucurbita_argyrosperma_scaffold_337 : 21928 .. 24871 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTATGTACGGGCGAGATCCGTGGGGGGGTCCGCTTGAGATTAACACGGCGGATTCGGCTACCGACGACGATCGCAGCCGGAATTTGCAGGATTTGGACAGGGCCGCTCTGTCTCGGCCTCTCGATGAGACTCAACAGAGCTGGCTGCTTGGACCTGGCGAACAGAAGAAGAAGAAGTATGTGGATCTCGGCTGCATTATTGTCAGTCGGAAGATCTTTGTGTGGACTGTTGGAACCCTACTTGTATCTGGGTTTCTCGCTGGTCTTATTACCCTAATCGTCAAAACGGTGCCGCGCCACCACCACCCCCACCCGCCGCCGGATAATTACACTCTTGCCCTCCATAAGGCACTCATGTTCTTCAACGCTCAGCGATGTATGTCCATCTTGCATCTCTTACTACTGCTGCCCTTGATTTTGATCATCGTGGTTTTAGATTGTTTTGCTTTCTGTCTGTTGAAAATTTGGGATGCGTAGTTAAGGAAATTAGTTGATGATGAAAACGTTTGTTGGTTATCTCAAGATCTATATGTGAATTGATGATTGCCTCGGTGAATAAATCATCCTGTCAATTTCGAAATTTATTACTGGCTAACCCCCGTACATTACAGAACTTGAAATTTGACCCTGTTACCAATTAAGTTCTGATGAAACGGGGGACTGGATTTGTATTTGTAGAGTTGGGCAGTAAAGATTTAGCTTTATGTTAGTTGTCGTTTGATAATCAGGAGAGCACATGATATGGTGTTGTGTTATTTACTTCATTTTCGTTCATGAATGTAGCTGGTAAGCTCCCGAAGCACAATAATGTGTCCTGGAGGGGTAATTCCTGCACGCAAGATGGCGATGATTCATCTCTCTTTAAAGATCTGTCTGGTGGCTATTATGACGCTGGAGATGCAATTAAGTTCAATTTTCCTGCCTCTTTTGCCATGACCATGTTAAGCTGGAGTGTCATTGAATACAGTGCCAAATATGAAGCTGCTGGAGAGCTCAACCATGTCAAGGATATTATTAAGTGGGGAAGTGATTATTTCCTCAAGACTTTTAACCATACTGCTGATTCTATCGGTACCATCGCCATGCAGGTATGAAAAACTCACTCATGTTTAATTCTTATTTAATGTCGTAGATGACTTGAAGTGAATTATGAATGAATTATGAATTTGAAACGTAGTGTGTATGAATAATCTGTATCTTCATTTGCATGAGTCTGGTTTTTGTTTAGTTGTGCCCCTATAATTTTATTCTGAGTCTTTTGTTCAGCGACGATAAACAAAAATTCTATCCATCATATGACAGGTTGGAGTAGGGGATACCTCTGGAGGAAATACAAGTCCAAACGATCATTATTGCTGGATGCGCCCAGAGGACATTGATTATGTGCGGCCTGTAATTACGTGCAGTAGTTGCTCAGATCTTGCTGCCGAAATGGCTGCTGCTTTAGCTTCAGCATCGATTGTTTTCAAAGACAACAAGGCATACTCACAGAAGCTTGTCCATGGTGCCAGAACACTCTTCAAGTTTGCCAGGGATCAGAGAGGCAGATACAGTGGAGGTAATTCAGAAGCTTCAATCTTCTACAATTCAACTAGTTATTGGGATGAGTTTGTATGGGGTGGAGCTTGGTTGTATTATGCTACTGGGAATTCCAGTTATCTTCAACTTTCTACCACTCCTGGCATTGCCAAACATGCGGGTGCGTTCTGGGGTGGACCTGACTATGGAGTTTTGAGCTGGGACAACAAGCTTGCAGGCGCCCAGGTTCATTTACGAATTCTCATTGTCCCTGTTTTCTAATTTTTATTCTTAAAATAATGTGGCATTTCTCTTATACTTGTGATGAATTATAATACGTTCTTGTCACAATATTGAAGGGTACTCGTTGGTTTCTTGCTATCCATTATGGGGTGTACTTTGCTGAAATTCCTTGTACCATTTCTTGTAGGTTCTTCTCAGTCGTTTGCGATTGTTCTTGAGCCCTGGATATCCATATGAGGAAATCTTGAGGACATTCCACAACCAAACTAGCATTGTTATGTGTTCCTACCTACCATTTTTCACAAAGTTTAATCGAACTAGAGGTACACCACTCATTGGAAATTGAACTCTTTTAACCTTACAGTTACTCATCATCATTAGTTATAATTTACGAATATAGTTTACATTTTTGCTATCTTGTTGGGGTGATCTTATAGGAGGCCTAATTCAGCTAAACCATGGAAGGCCACAGCCTCTTCAGTACGTCGTCAATGCAGCCTTTTTAGCTACCCTGTACAGTGATTACTTGGAAGCGGCAGATACACCTGGATGGTACTGTGGGCCCAATTTCTATTCAACTGATGTTTTGCGCGATTTTGCGAAGACCCAGGTATTTCATCCTTCTTTACACATTATGTTGAACTGCTTACTGAACATATAAGATTAATTGAACCGAATTGACATGAGTATGAATTGGATTGGGTTGGTTCATCAGTTTAGTTCAGTTTCTTACCCTCAATACACGCTCAACTCAACACTATTCTTGTGTGTTCTGCAGATTGATTATATCCTTGGCAAAAACCCGCGCAAGATGAGCTACATCGTTGGTTTTGGCAATCATTATCCGAAACATGTGCATCATAGAGGTGCATCAATCCCAAAGAACAAGATCAAATATAACTGCAAAGGAGGATGGAAATGGAGGGATACAAAAAAGCCTAACCCAAATACAGTTGTTGGTGCCATGGTTGCTGGTCCTGACAAGCACGATGGGTTTCACGATGTTCGCACCAACTATAACTACACTGAGCCAACTCTAGCTGGAAATGCAGGCTTGGTTGCAGCACTAGTTGCTCTTTCGGGTGAAAAAACATCTGGAATTGACAAGAACACCATCTTCTCTGCAGTGCCTCCAATGTTCCCAACTCCACCACCTCCTCCAGCACCTTGGAAACCTTGA

mRNA sequence

ATGAGTATGTACGGGCGAGATCCGTGGGGGGGTCCGCTTGAGATTAACACGGCGGATTCGGCTACCGACGACGATCGCAGCCGGAATTTGCAGGATTTGGACAGGGCCGCTCTGTCTCGGCCTCTCGATGAGACTCAACAGAGCTGGCTGCTTGGACCTGGCGAACAGAAGAAGAAGAAGTATGTGGATCTCGGCTGCATTATTGTCAGTCGGAAGATCTTTGTGTGGACTGTTGGAACCCTACTTGTATCTGGGTTTCTCGCTGGTCTTATTACCCTAATCGTCAAAACGGTGCCGCGCCACCACCACCCCCACCCGCCGCCGGATAATTACACTCTTGCCCTCCATAAGGCACTCATGTTCTTCAACGCTCAGCGATCTGGTAAGCTCCCGAAGCACAATAATGTGTCCTGGAGGGGTAATTCCTGCACGCAAGATGGCGATGATTCATCTCTCTTTAAAGATCTGTCTGGTGGCTATTATGACGCTGGAGATGCAATTAAGTTCAATTTTCCTGCCTCTTTTGCCATGACCATGTTAAGCTGGAGTGTCATTGAATACAGTGCCAAATATGAAGCTGCTGGAGAGCTCAACCATGTCAAGGATATTATTAAGTGGGGAAGTGATTATTTCCTCAAGACTTTTAACCATACTGCTGATTCTATCGGTACCATCGCCATGCAGGTTGGAGTAGGGGATACCTCTGGAGGAAATACAAGTCCAAACGATCATTATTGCTGGATGCGCCCAGAGGACATTGATTATGTGCGGCCTGTAATTACGTGCAGTAGTTGCTCAGATCTTGCTGCCGAAATGGCTGCTGCTTTAGCTTCAGCATCGATTGTTTTCAAAGACAACAAGGCATACTCACAGAAGCTTGTCCATGGTGCCAGAACACTCTTCAAGTTTGCCAGGGATCAGAGAGGCAGATACAGTGGAGGTAATTCAGAAGCTTCAATCTTCTACAATTCAACTAGTTATTGGGATGAGTTTGTATGGGGTGGAGCTTGGTTGTATTATGCTACTGGGAATTCCAGTTATCTTCAACTTTCTACCACTCCTGGCATTGCCAAACATGCGGGTGCGTTCTGGGGTGGACCTGACTATGGAGTTTTGAGCTGGGACAACAAGCTTGCAGGCGCCCAGGTTCTTCTCAGTCGTTTGCGATTGTTCTTGAGCCCTGGATATCCATATGAGGAAATCTTGAGGACATTCCACAACCAAACTAGCATTGTTATGTGTTCCTACCTACCATTTTTCACAAAGTTTAATCGAACTAGAGGAGGCCTAATTCAGCTAAACCATGGAAGGCCACAGCCTCTTCAGTACGTCGTCAATGCAGCCTTTTTAGCTACCCTGTACAGTGATTACTTGGAAGCGGCAGATACACCTGGATGGTACTGTGGGCCCAATTTCTATTCAACTGATGTTTTGCGCGATTTTGCGAAGACCCAGATTGATTATATCCTTGGCAAAAACCCGCGCAAGATGAGCTACATCGTTGGTTTTGGCAATCATTATCCGAAACATGTGCATCATAGAGGTGCATCAATCCCAAAGAACAAGATCAAATATAACTGCAAAGGAGGATGGAAATGGAGGGATACAAAAAAGCCTAACCCAAATACAGTTGTTGGTGCCATGGTTGCTGGTCCTGACAAGCACGATGGGTTTCACGATGTTCGCACCAACTATAACTACACTGAGCCAACTCTAGCTGGAAATGCAGGCTTGGTTGCAGCACTAGTTGCTCTTTCGGGTGAAAAAACATCTGGAATTGACAAGAACACCATCTTCTCTGCAGTGCCTCCAATGTTCCCAACTCCACCACCTCCTCCAGCACCTTGGAAACCTTGA

Coding sequence (CDS)

ATGAGTATGTACGGGCGAGATCCGTGGGGGGGTCCGCTTGAGATTAACACGGCGGATTCGGCTACCGACGACGATCGCAGCCGGAATTTGCAGGATTTGGACAGGGCCGCTCTGTCTCGGCCTCTCGATGAGACTCAACAGAGCTGGCTGCTTGGACCTGGCGAACAGAAGAAGAAGAAGTATGTGGATCTCGGCTGCATTATTGTCAGTCGGAAGATCTTTGTGTGGACTGTTGGAACCCTACTTGTATCTGGGTTTCTCGCTGGTCTTATTACCCTAATCGTCAAAACGGTGCCGCGCCACCACCACCCCCACCCGCCGCCGGATAATTACACTCTTGCCCTCCATAAGGCACTCATGTTCTTCAACGCTCAGCGATCTGGTAAGCTCCCGAAGCACAATAATGTGTCCTGGAGGGGTAATTCCTGCACGCAAGATGGCGATGATTCATCTCTCTTTAAAGATCTGTCTGGTGGCTATTATGACGCTGGAGATGCAATTAAGTTCAATTTTCCTGCCTCTTTTGCCATGACCATGTTAAGCTGGAGTGTCATTGAATACAGTGCCAAATATGAAGCTGCTGGAGAGCTCAACCATGTCAAGGATATTATTAAGTGGGGAAGTGATTATTTCCTCAAGACTTTTAACCATACTGCTGATTCTATCGGTACCATCGCCATGCAGGTTGGAGTAGGGGATACCTCTGGAGGAAATACAAGTCCAAACGATCATTATTGCTGGATGCGCCCAGAGGACATTGATTATGTGCGGCCTGTAATTACGTGCAGTAGTTGCTCAGATCTTGCTGCCGAAATGGCTGCTGCTTTAGCTTCAGCATCGATTGTTTTCAAAGACAACAAGGCATACTCACAGAAGCTTGTCCATGGTGCCAGAACACTCTTCAAGTTTGCCAGGGATCAGAGAGGCAGATACAGTGGAGGTAATTCAGAAGCTTCAATCTTCTACAATTCAACTAGTTATTGGGATGAGTTTGTATGGGGTGGAGCTTGGTTGTATTATGCTACTGGGAATTCCAGTTATCTTCAACTTTCTACCACTCCTGGCATTGCCAAACATGCGGGTGCGTTCTGGGGTGGACCTGACTATGGAGTTTTGAGCTGGGACAACAAGCTTGCAGGCGCCCAGGTTCTTCTCAGTCGTTTGCGATTGTTCTTGAGCCCTGGATATCCATATGAGGAAATCTTGAGGACATTCCACAACCAAACTAGCATTGTTATGTGTTCCTACCTACCATTTTTCACAAAGTTTAATCGAACTAGAGGAGGCCTAATTCAGCTAAACCATGGAAGGCCACAGCCTCTTCAGTACGTCGTCAATGCAGCCTTTTTAGCTACCCTGTACAGTGATTACTTGGAAGCGGCAGATACACCTGGATGGTACTGTGGGCCCAATTTCTATTCAACTGATGTTTTGCGCGATTTTGCGAAGACCCAGATTGATTATATCCTTGGCAAAAACCCGCGCAAGATGAGCTACATCGTTGGTTTTGGCAATCATTATCCGAAACATGTGCATCATAGAGGTGCATCAATCCCAAAGAACAAGATCAAATATAACTGCAAAGGAGGATGGAAATGGAGGGATACAAAAAAGCCTAACCCAAATACAGTTGTTGGTGCCATGGTTGCTGGTCCTGACAAGCACGATGGGTTTCACGATGTTCGCACCAACTATAACTACACTGAGCCAACTCTAGCTGGAAATGCAGGCTTGGTTGCAGCACTAGTTGCTCTTTCGGGTGAAAAAACATCTGGAATTGACAAGAACACCATCTTCTCTGCAGTGCCTCCAATGTTCCCAACTCCACCACCTCCTCCAGCACCTTGGAAACCTTGA

Protein sequence

MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTMLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTSPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFSAVPPMFPTPPPPPAPWKP
BLAST of Carg25859 vs. NCBI nr
Match: XP_022942996.1 (endoglucanase 25-like [Cucurbita moschata])

HSP 1 Score: 1283.1 bits (3319), Expect = 0.0e+00
Identity = 618/618 (100.00%), Postives = 618/618 (100.00%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 180
           FFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML
Sbjct: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 180

Query: 181 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 240
           SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS
Sbjct: 181 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 240

Query: 241 PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 300
           PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL
Sbjct: 241 PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 300

Query: 301 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 360
           FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA
Sbjct: 301 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 360

Query: 361 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 420
           GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF
Sbjct: 361 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 420

Query: 421 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 480
           TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD
Sbjct: 421 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 480

Query: 481 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 540
           FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN
Sbjct: 481 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 540

Query: 541 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 600
           PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS
Sbjct: 541 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 600

Query: 601 AVPPMFPTXXXXXAPWKP 619
           AVPPMFPTXXXXXAPWKP
Sbjct: 601 AVPPMFPTXXXXXAPWKP 618

BLAST of Carg25859 vs. NCBI nr
Match: XP_023542874.1 (endoglucanase 25-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1280.8 bits (3313), Expect = 0.0e+00
Identity = 617/618 (99.84%), Postives = 617/618 (99.84%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 180
           FFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML
Sbjct: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 180

Query: 181 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 240
           SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSI TIAMQVGVGDTSGGNTS
Sbjct: 181 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSISTIAMQVGVGDTSGGNTS 240

Query: 241 PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 300
           PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL
Sbjct: 241 PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 300

Query: 301 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 360
           FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA
Sbjct: 301 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 360

Query: 361 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 420
           GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF
Sbjct: 361 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 420

Query: 421 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 480
           TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD
Sbjct: 421 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 480

Query: 481 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 540
           FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN
Sbjct: 481 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 540

Query: 541 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 600
           PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS
Sbjct: 541 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 600

Query: 601 AVPPMFPTXXXXXAPWKP 619
           AVPPMFPTXXXXXAPWKP
Sbjct: 601 AVPPMFPTXXXXXAPWKP 618

BLAST of Carg25859 vs. NCBI nr
Match: XP_022977523.1 (endoglucanase 25-like [Cucurbita maxima])

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 615/618 (99.51%), Postives = 617/618 (99.84%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 180
           FFNAQRSGKLPKHNNVSWRGNSCTQDGDD+SLFKDLSGGYYDAGDAIKFNFPASFAMTML
Sbjct: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGDDTSLFKDLSGGYYDAGDAIKFNFPASFAMTML 180

Query: 181 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 240
           SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSI TIAMQVGVGDTSGGNTS
Sbjct: 181 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSISTIAMQVGVGDTSGGNTS 240

Query: 241 PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 300
           PNDHYCWMRPEDIDY+RPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL
Sbjct: 241 PNDHYCWMRPEDIDYMRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 300

Query: 301 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 360
           FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA
Sbjct: 301 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 360

Query: 361 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 420
           GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF
Sbjct: 361 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 420

Query: 421 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 480
           TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD
Sbjct: 421 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 480

Query: 481 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 540
           FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN
Sbjct: 481 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 540

Query: 541 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 600
           PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS
Sbjct: 541 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 600

Query: 601 AVPPMFPTXXXXXAPWKP 619
           AVPPMFPTXXXXXAPWKP
Sbjct: 601 AVPPMFPTXXXXXAPWKP 618

BLAST of Carg25859 vs. NCBI nr
Match: XP_022136360.1 (endoglucanase 25 [Momordica charantia])

HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 597/619 (96.45%), Postives = 610/619 (98.55%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEIN ADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINAADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVSGF+AGLITLIVKTVPRHHHPHPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFIAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGD-DSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 180
           FFNAQRSGKLPKHNNVSWR NSCT+DGD  SSLFKDLSGG+YDAGDAIKFNFPASFAMTM
Sbjct: 121 FFNAQRSGKLPKHNNVSWRXNSCTRDGDGSSSLFKDLSGGFYDAGDAIKFNFPASFAMTM 180

Query: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNT 240
           LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLK+FNHTADS+ TIAMQVGVGDTSGGNT
Sbjct: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKSFNHTADSVNTIAMQVGVGDTSGGNT 240

Query: 241 SPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300
           SPNDHYCWMRPEDIDYVRPV TCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART
Sbjct: 241 SPNDHYCWMRPEDIDYVRPVQTCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300

Query: 301 LFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 360
           LFKFAR+QRGRYS GNSEA+IFYNSTSYWDEFVWGGAWLYYATGNSSYLQL+TTPGIAKH
Sbjct: 301 LFKFAREQRGRYSAGNSEAAIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLATTPGIAKH 360

Query: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420
           AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF
Sbjct: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420

Query: 421 FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR 480
           FTKFNRTRGGLIQLNHGRPQPLQY+VNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR
Sbjct: 421 FTKFNRTRGGLIQLNHGRPQPLQYIVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR 480

Query: 481 DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKP 540
           DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRD+ KP
Sbjct: 481 DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDSTKP 540

Query: 541 NPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIF 600
           NPNT+VGAMVAGPDKHD FHDVRTNYNYTEPTLAGNAGLVAAL+ALSGEKT+GIDKNTIF
Sbjct: 541 NPNTIVGAMVAGPDKHDRFHDVRTNYNYTEPTLAGNAGLVAALIALSGEKTTGIDKNTIF 600

Query: 601 SAVPPMFPTXXXXXAPWKP 619
           SAVPPMFPTXXXXXAPWKP
Sbjct: 601 SAVPPMFPTXXXXXAPWKP 619

BLAST of Carg25859 vs. NCBI nr
Match: NP_001284449.1 (endoglucanase 25-like [Cucumis melo] >BAF30815.1 endoglucanase [Cucumis melo])

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 599/622 (96.30%), Postives = 610/622 (98.07%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEIN ADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINAADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGD-DSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 180
           FFNAQRSGKLPKHNNVSWRGNSCT+DGD  SSLFKDLSGGYYDAGDAIKFNFPASFAMTM
Sbjct: 121 FFNAQRSGKLPKHNNVSWRGNSCTRDGDGSSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 180

Query: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNT 240
           LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSI TI MQVGVGDTSGGNT
Sbjct: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSISTIVMQVGVGDTSGGNT 240

Query: 241 SPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300
           SPNDHYCWMRPEDIDYVRPV+TCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART
Sbjct: 241 SPNDHYCWMRPEDIDYVRPVLTCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300

Query: 301 LFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 360
           LFKFAR+QRGRYS GN+EA+IFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH
Sbjct: 301 LFKFAREQRGRYSAGNAEAAIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 360

Query: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420
           AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF
Sbjct: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420

Query: 421 FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR 480
           FTKFNRTRGGLIQLNHGRPQPLQY+VNAAFLATLYSDYLEAADTPGWYCGPNFYST+VLR
Sbjct: 421 FTKFNRTRGGLIQLNHGRPQPLQYIVNAAFLATLYSDYLEAADTPGWYCGPNFYSTEVLR 480

Query: 481 DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKP 540
           DFAKTQIDYILGKNPRKMSY+VGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDT KP
Sbjct: 481 DFAKTQIDYILGKNPRKMSYVVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTTKP 540

Query: 541 NPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSG---EKTSGIDKN 600
           NPNT+VGAMVAGPD+ DGFHDVRTNYNYTEPTLAGNAGLVAALVALSG   EK +GIDKN
Sbjct: 541 NPNTLVGAMVAGPDRRDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGENSEKATGIDKN 600

Query: 601 TIFSAVPPMFPTXXXXXAPWKP 619
           TIFSAVPPMFPTXXXXXAPWKP
Sbjct: 601 TIFSAVPPMFPTXXXXXAPWKP 622

BLAST of Carg25859 vs. TAIR10
Match: AT5G49720.1 (glycosyl hydrolase 9A1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 515/621 (82.93%), Postives = 556/621 (89.53%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 62
           MYGRDPWGGPLEINTADSATDDDRSRNL DLDRAALSRPLDETQQSWLLGP EQKKKKYV
Sbjct: 1   MYGRDPWGGPLEINTADSATDDDRSRNLNDLDRAALSRPLDETQQSWLLGPTEQKKKKYV 60

Query: 63  DLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALMFF 122
           DLGCIIVSRKIFVWTVGTL+ +  LAG ITLIVKTVPRHH   PPPDNYT+ALHKAL FF
Sbjct: 61  DLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIALHKALKFF 120

Query: 123 NAQRSGKLPKHNNVSWRGNSCTQD--GDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 182
           NAQ+SGKLPKHNNVSWRGNS  QD  G+  S +KDL GGYYDAGDAIKFNFP ++AMTML
Sbjct: 121 NAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAGDAIKFNFPMAYAMTML 180

Query: 183 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 242
           SWSVIEYSAKYEAAGEL HVK++IKWG+DYFLKTFN TADSI  +  QVG G+T  GNT 
Sbjct: 181 SWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTFNSTADSIDDLVSQVGSGNTDDGNTD 240

Query: 243 PNDHYCWMRPEDIDYVRPVITCS-SCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 302
           PNDHYCWMRPED+DY RPV TC+  CSDLAAEMAAALASASIVFKDNK YS+KLVHGA+ 
Sbjct: 241 PNDHYCWMRPEDMDYKRPVTTCNGGCSDLAAEMAAALASASIVFKDNKEYSKKLVHGAKV 300

Query: 303 LFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 362
           +++F R +RGRYS G +E+S FYNS+ YWDEF+WGGAW+YYATGN +YL L T P +AKH
Sbjct: 301 VYQFGRTRRGRYSAGTAESSKFYNSSMYWDEFIWGGAWMYYATGNVTYLNLITQPTMAKH 360

Query: 363 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 422
           AGAFWGGP YGV SWDNKLAGAQ+LLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLP 
Sbjct: 361 AGAFWGGPYYGVFSWDNKLAGAQLLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPI 420

Query: 423 FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR 482
           F KFNRT GGLI+LNHG PQPLQY VNAAFLATLYSDYL+AADTPGWYCGPNFYST VLR
Sbjct: 421 FNKFNRTNGGLIELNHGAPQPLQYSVNAAFLATLYSDYLDAADTPGWYCGPNFYSTSVLR 480

Query: 483 DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKP 542
           DFA++QIDYILGKNPRKMSY+VGFG  YP+HVHHRGASIPKNK+KYNCKGGWKWRD+KKP
Sbjct: 481 DFARSQIDYILGKNPRKMSYVVGFGTKYPRHVHHRGASIPKNKVKYNCKGGWKWRDSKKP 540

Query: 543 NPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEK--TSGIDKNT 602
           NPNT+ GAMVAGPDK DG+ DVR NYNYTEPTLAGNAGLVAALVALSGE+  T  IDKNT
Sbjct: 541 NPNTIEGAMVAGPDKRDGYRDVRMNYNYTEPTLAGNAGLVAALVALSGEEEATGKIDKNT 600

Query: 603 IFSAVPPMFPTXXXXXAPWKP 619
           IFSAVPP+FPT XXXXAPWKP
Sbjct: 601 IFSAVPPLFPTPXXXXAPWKP 621

BLAST of Carg25859 vs. TAIR10
Match: AT4G24260.1 (glycosyl hydrolase 9A3)

HSP 1 Score: 913.3 bits (2359), Expect = 8.1e-266
Identity = 445/621 (71.66%), Postives = 507/621 (81.64%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 62
           MYGRDPWGGPLEIN ADS TDDDRSRNLQDLDRA  SRPLDETQQSWLLGP   KKKKYV
Sbjct: 1   MYGRDPWGGPLEINAADSMTDDDRSRNLQDLDRATPSRPLDETQQSWLLGPKVLKKKKYV 60

Query: 63  DLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALMFF 122
           DLGCI+VSRKIF+WT+GT++V+  L+G ITLIVKT+           NYT+AL  AL FF
Sbjct: 61  DLGCILVSRKIFLWTLGTIVVTALLSGFITLIVKTLXXXXXXXXXXXNYTIALRTALKFF 120

Query: 123 NAQRSGKLPKH-NNVSWRGNSCTQD--GDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 182
           NAQ+SGKLPK+  NVSWR +SC QD  GD    +KDL GGYYDAGD+IKFNFP S+AMTM
Sbjct: 121 NAQQSGKLPKNIYNVSWRHDSCLQDGKGDPGQCYKDLVGGYYDAGDSIKFNFPMSYAMTM 180

Query: 183 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNT 242
           LSWSVIEYSAKY+AAGEL HVK++IKWG+DYFLKTFN +AD+I  +  QVG G +  G+ 
Sbjct: 181 LSWSVIEYSAKYQAAGELEHVKELIKWGTDYFLKTFNSSADNIYVMVEQVGSGVSGRGSE 240

Query: 243 SPNDHYCWMRPEDIDYVRPVITC-SSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAR 302
             NDHYCWMRPEDI Y R V  C SSCSDLAAEMAAALASASIVFKDN+ YS+ LVHGA+
Sbjct: 241 LHNDHYCWMRPEDIHYKRTVSQCYSSCSDLAAEMAAALASASIVFKDNRLYSKNLVHGAK 300

Query: 303 TLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAK 362
           TL++FA   R RYS    E+S FYNS+ + DE +WGGAWLYYATGN +YL+  T+  +A+
Sbjct: 301 TLYRFATTSRNRYSQNGKESSKFYNSSMFEDELLWGGAWLYYATGNVTYLERVTSHHMAE 360

Query: 363 HAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLP 422
            AGAF   P YGV SWDNKL GAQ+LL+R+RLFLSPGYPYE++L  FHNQT  VMCSYLP
Sbjct: 361 KAGAFGNSPYYGVFSWDNKLPGAQLLLTRMRLFLSPGYPYEDMLSEFHNQTGRVMCSYLP 420

Query: 423 FFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVL 482
           ++ KFNRT GGLIQLNHG PQPLQYV NAAFLA L+SDYLEAADTPGWYCGPNFY+T+ L
Sbjct: 421 YYKKFNRTNGGLIQLNHGAPQPLQYVANAAFLAALFSDYLEAADTPGWYCGPNFYTTEFL 480

Query: 483 RDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKK 542
           R+F+++QIDYILGKNPRKMSY+VG+G  YPK VHHRGASIPKN +K  C GG+KW+ +KK
Sbjct: 481 RNFSRSQIDYILGKNPRKMSYVVGYGQRYPKQVHHRGASIPKN-MKETCTGGFKWKKSKK 540

Query: 543 PNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEK-TSGIDKNT 602
            NPN + GAMVAGPDKHDGFHD+RTNYNYTEPTLAGNAGLVAALVALSGEK   GIDKNT
Sbjct: 541 NNPNAINGAMVAGPDKHDGFHDIRTNYNYTEPTLAGNAGLVAALVALSGEKAVGGIDKNT 600

Query: 603 IFSAVPPMFPTXXXXXAPWKP 619
           +FSAVPP+        APW P
Sbjct: 601 MFSAVPPLVMATPPPPAPWTP 620

BLAST of Carg25859 vs. TAIR10
Match: AT1G65610.1 (Six-hairpin glycosidases superfamily protein)

HSP 1 Score: 705.7 bits (1820), Expect = 2.6e-203
Identity = 342/626 (54.63%), Postives = 453/626 (72.36%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTAD--SATDDDRSRNLQDLDRAAL---SRPLDETQQSWLLGPGE-- 62
           M+  + WGG L+   +D  +A +++R RN  + DR A+      LDETQQ WLL P +  
Sbjct: 1   MHPGNVWGGSLDAVDSDRIAAEEEERLRNTTEWDRGAIHSQRSELDETQQGWLLAPQDNW 60

Query: 63  -QKKKKYVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLA 122
            +KKKKYV+LGC+ VSR +F+WTVG++ V   +  L  +IVK++PRH    PPPDNYTLA
Sbjct: 61  RKKKKKYVNLGCVSVSRTVFLWTVGSIAVLFLVVALPIIIVKSLPRHKSAPPPPDNYTLA 120

Query: 123 LHKALMFFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKFNFPAS 182
           LHKAL FF+AQ+SGKLPK N VSWRG+S T+DG    +   L GGYYD G  +KF+FP +
Sbjct: 121 LHKALQFFDAQKSGKLPKKNKVSWRGDSGTKDG-LPDVVGGLVGGYYDGGSNVKFHFPMA 180

Query: 183 FAMTMLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDT 242
           F+MTMLSWS+IEYS KY+A  E +H++D++KWG+DY L TFN++A  +  I  QVG G  
Sbjct: 181 FSMTMLSWSLIEYSHKYKAIDEYDHMRDVLKWGTDYLLLTFNNSATRLDHIYTQVGGG-- 240

Query: 243 SGGNTSPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLV 302
              + SP+D YCW +PED+ Y RPV++ +S +DL AE++AALA+ASIVF D   Y++KL 
Sbjct: 241 LRDSESPDDIYCWQKPEDMSYDRPVLSSTSAADLGAEVSAALAAASIVFTDKPDYAKKLK 300

Query: 303 HGARTLFKF--ARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLST 362
            GA TL+ F  ++ +R RYS G   A  FYNSTS +DEF+W GAWLYYATGN +Y+Q +T
Sbjct: 301 KGAETLYPFFRSKSRRKRYSDGQPTAQAFYNSTSMFDEFMWAGAWLYYATGNKTYIQFAT 360

Query: 363 TPGIAKHAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIV 422
           TP + + A AF   P+  V SW+NKL GA +L++R RLFL+PG+PYE +L  +HN T I 
Sbjct: 361 TPSVPQTAKAFANRPELMVPSWNNKLPGAMLLMTRYRLFLNPGFPYENMLNRYHNATGIT 420

Query: 423 MCSYLPFFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNF 482
           MC+YL  +  FNRT GGL+QLN G+P+PL+YV +A+FLA+L++DYL +   PGWYCGP F
Sbjct: 421 MCAYLKQYNVFNRTSGGLMQLNLGKPRPLEYVAHASFLASLFADYLNSTGVPGWYCGPTF 480

Query: 483 YSTDVLRDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWK 542
               VL+DFA++QIDYILG NP KMSY+VGFG  +P+ VHHRGA+IP +K + +C+ G K
Sbjct: 481 VENHVLKDFAQSQIDYILGDNPLKMSYVVGFGKKFPRRVHHRGATIPNDKKRRSCREGLK 540

Query: 543 WRDTKKPNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSG 602
           +RDTK PNPN + GAMV GP+K D FHD+R NYN +EPTL+GNAGLVAALV+L+      
Sbjct: 541 YRDTKNPNPNNITGAMVGGPNKFDEFHDLRNNYNASEPTLSGNAGLVAALVSLTSSGGQQ 600

Query: 603 IDKNTIFSAVPPMFPTXXXXXAPWKP 619
           IDKNT+F++VPP++         WKP
Sbjct: 601 IDKNTMFNSVPPLYSPTPPPPKAWKP 623

BLAST of Carg25859 vs. TAIR10
Match: AT1G75680.1 (glycosyl hydrolase 9B7)

HSP 1 Score: 351.3 bits (900), Expect = 1.2e-96
Identity = 213/491 (43.38%), Postives = 287/491 (58.45%), Query Frame = 0

Query: 104 PHPP---PDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGY 163
           P PP      Y  AL  AL FF+ Q+SGKL ++N + WRG+S  +DG + +L  DLS G 
Sbjct: 48  PGPPGAIDKKYADALKLALQFFDIQKSGKL-ENNKIPWRGDSGLKDGSEDNL--DLSKGL 107

Query: 164 YDAGDAIKFNFPASFAMTMLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTAD 223
           YDAGD IKF FP +F  T+LSWS++EY  +  A  +L+  KD ++W +DY +K   H +D
Sbjct: 108 YDAGDHIKFGFPMAFTATVLSWSILEYGDQMNAVNQLDPAKDSLRWITDYLIKA--HPSD 167

Query: 224 SIGTIAMQVGVGDTSGGNTSPNDHYCWMRPEDIDYVRPVITC---SSCSDLAAEMAAALA 283
           ++    + + VGD         DH CW RPED+   RP+      +  +++AAE AAA+A
Sbjct: 168 NV----LYIQVGDPK------VDHPCWERPEDMKEKRPLTKIDVDTPGTEVAAETAAAMA 227

Query: 284 SASIVFKD-NKAYSQKLVHGARTLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGA 343
           SAS+VFKD +  YS  L+  A+ LF FA  +RG YS    E   FYNST Y DE +W  +
Sbjct: 228 SASLVFKDSDPTYSATLLKHAKQLFNFADTKRGSYSVNIPEVQKFYNSTGYGDELLWAAS 287

Query: 344 WLYYATGNSSYLQLSTTPGIAKHAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGY 403
           WLY+AT + +YL   +  G  K   +F G P +   SWDNKLAG QVLLSRL LF     
Sbjct: 288 WLYHATEDKTYLDYVSNHG--KEFASF-GNPTW--FSWDNKLAGTQVLLSRL-LFFKKDL 347

Query: 404 PYEEILRTFHNQTSIVMCSYLPF--FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLY 463
              + L  + N    VMC  LP    +  +RT GGLI ++      +Q  V++AFLA+L+
Sbjct: 348 SGSKGLGNYRNTAKAVMCGLLPKSPTSTASRTNGGLIWVSEW--NSMQQSVSSAFLASLF 407

Query: 464 SDYLEAADTPGWYCGPNFYSTDVLRDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHR 523
           SDY+  +      C    +    LRDFAK+Q DY+LGKNP   S++VG+G+ YP+ VHHR
Sbjct: 408 SDYMLTSRIHKISCDGKIFKATELRDFAKSQADYMLGKNPLGTSFVVGYGDKYPQFVHHR 467

Query: 524 GASIPKNKIKYNCKGGWKWRDTKKPNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAG 583
           GASIP +     C  G+KW ++ KPNPN   GA+V GP  ++ F D R N    EPT   
Sbjct: 468 GASIPADATT-GCLDGFKWFNSTKPNPNIAYGALVGGPFFNETFTDSRENPMQNEPTTYN 514

Query: 584 NAGLVAALVAL 586
           NA LV  L +L
Sbjct: 528 NALLVGLLSSL 514

BLAST of Carg25859 vs. TAIR10
Match: AT1G19940.1 (glycosyl hydrolase 9B5)

HSP 1 Score: 347.4 bits (890), Expect = 1.8e-95
Identity = 205/491 (41.75%), Postives = 287/491 (58.45%), Query Frame = 0

Query: 110 NYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFKDLSGGYYDAGDAIKF 169
           NY  AL  A+ FF+ Q+SGKL ++N +SWRG+S  +DG ++S+  DLS G YDAGD +KF
Sbjct: 48  NYANALKIAMQFFDIQKSGKL-ENNEISWRGDSGLKDGSEASI--DLSKGLYDAGDHMKF 107

Query: 170 NFPASFAMTMLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQV 229
            FP +F  T+LSWS++EY  +  +   L+H KD +KW +D+ +    H + ++    + +
Sbjct: 108 GFPMAFTATVLSWSILEYGDQMASLNLLDHAKDSLKWTTDFLINA--HPSPNV----LYI 167

Query: 230 GVGDTSGGNTSPNDHYCWMRPEDIDYVR---PVITCSSCSDLAAEMAAALASASIVFKDN 289
            VGD         DH CW RPE +   R    + T +  +++AAE AAA+A+AS+VFK++
Sbjct: 168 QVGD------PVTDHKCWDRPETMTRKRTLTKIDTKTPGTEVAAETAAAMAAASLVFKES 227

Query: 290 KA-YSQKLVHGARTLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNS 349
              YS  L+  A+ LF FA + RG YS    E   +YNST Y DE +W  +WLY+AT + 
Sbjct: 228 DTKYSSTLLKHAKQLFDFADNNRGSYSVNIPEVQSYYNSTGYGDELLWAASWLYHATEDQ 287

Query: 350 SYLQLSTTPGIAKHAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTF 409
           +YL   +  G  +  G F G P +   SWDNKL G  +LLSRL  F   G    + L+ F
Sbjct: 288 TYLDFVSENG--EEFGNF-GSPSW--FSWDNKLPGTHILLSRL-TFFKKGLSGSKGLQGF 347

Query: 410 HNQTSIVMCSYLPF--FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADT 469
                 VMC  +P       +RT GGLI ++      LQ+ V++AFLATLYSDY+  +  
Sbjct: 348 KETAEAVMCGLIPSSPTATSSRTDGGLIWVSEW--NALQHPVSSAFLATLYSDYMLTSGV 407

Query: 470 PGWYCGPNFYSTDVLRDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKI 529
               C    +    LR FA++Q DY+LGKNP KMSY+VG+G  YP+ VHHRGASIP +  
Sbjct: 408 KELSCSDQSFKPSDLRKFARSQADYMLGKNPEKMSYLVGYGEKYPEFVHHRGASIPADAT 467

Query: 530 KYNCKGGWKWRDTKKPNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNA---GLVA 589
              CK G+KW ++ +PNPN   GA+V GP  +D F D R N    EP+   +A   GL++
Sbjct: 468 T-GCKDGFKWLNSDEPNPNVAYGALVGGPFLNDTFIDARNNSMQNEPSTYNSALVVGLLS 514

Query: 590 ALVALSGEKTS 592
           +LV  S    S
Sbjct: 528 SLVTTSSSVES 514

BLAST of Carg25859 vs. Swiss-Prot
Match: sp|Q38890|GUN25_ARATH (Endoglucanase 25 OS=Arabidopsis thaliana OX=3702 GN=KOR PE=1 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 515/621 (82.93%), Postives = 556/621 (89.53%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 62
           MYGRDPWGGPLEINTADSATDDDRSRNL DLDRAALSRPLDETQQSWLLGP EQKKKKYV
Sbjct: 1   MYGRDPWGGPLEINTADSATDDDRSRNLNDLDRAALSRPLDETQQSWLLGPTEQKKKKYV 60

Query: 63  DLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALMFF 122
           DLGCIIVSRKIFVWTVGTL+ +  LAG ITLIVKTVPRHH   PPPDNYT+ALHKAL FF
Sbjct: 61  DLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIALHKALKFF 120

Query: 123 NAQRSGKLPKHNNVSWRGNSCTQD--GDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 182
           NAQ+SGKLPKHNNVSWRGNS  QD  G+  S +KDL GGYYDAGDAIKFNFP ++AMTML
Sbjct: 121 NAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAGDAIKFNFPMAYAMTML 180

Query: 183 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 242
           SWSVIEYSAKYEAAGEL HVK++IKWG+DYFLKTFN TADSI  +  QVG G+T  GNT 
Sbjct: 181 SWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTFNSTADSIDDLVSQVGSGNTDDGNTD 240

Query: 243 PNDHYCWMRPEDIDYVRPVITCS-SCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 302
           PNDHYCWMRPED+DY RPV TC+  CSDLAAEMAAALASASIVFKDNK YS+KLVHGA+ 
Sbjct: 241 PNDHYCWMRPEDMDYKRPVTTCNGGCSDLAAEMAAALASASIVFKDNKEYSKKLVHGAKV 300

Query: 303 LFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 362
           +++F R +RGRYS G +E+S FYNS+ YWDEF+WGGAW+YYATGN +YL L T P +AKH
Sbjct: 301 VYQFGRTRRGRYSAGTAESSKFYNSSMYWDEFIWGGAWMYYATGNVTYLNLITQPTMAKH 360

Query: 363 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 422
           AGAFWGGP YGV SWDNKLAGAQ+LLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLP 
Sbjct: 361 AGAFWGGPYYGVFSWDNKLAGAQLLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPI 420

Query: 423 FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR 482
           F KFNRT GGLI+LNHG PQPLQY VNAAFLATLYSDYL+AADTPGWYCGPNFYST VLR
Sbjct: 421 FNKFNRTNGGLIELNHGAPQPLQYSVNAAFLATLYSDYLDAADTPGWYCGPNFYSTSVLR 480

Query: 483 DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKP 542
           DFA++QIDYILGKNPRKMSY+VGFG  YP+HVHHRGASIPKNK+KYNCKGGWKWRD+KKP
Sbjct: 481 DFARSQIDYILGKNPRKMSYVVGFGTKYPRHVHHRGASIPKNKVKYNCKGGWKWRDSKKP 540

Query: 543 NPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEK--TSGIDKNT 602
           NPNT+ GAMVAGPDK DG+ DVR NYNYTEPTLAGNAGLVAALVALSGE+  T  IDKNT
Sbjct: 541 NPNTIEGAMVAGPDKRDGYRDVRMNYNYTEPTLAGNAGLVAALVALSGEEEATGKIDKNT 600

Query: 603 IFSAVPPMFPTXXXXXAPWKP 619
           IFSAVPP+FPT XXXXAPWKP
Sbjct: 601 IFSAVPPLFPTPXXXXAPWKP 621

BLAST of Carg25859 vs. Swiss-Prot
Match: sp|P0C1U4|GUN9_ORYSJ (Endoglucanase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU1 PE=2 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 2.4e-291
Identity = 485/622 (77.97%), Postives = 536/622 (86.17%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLL-GPGEQ--K 60
           MSMYGRDPWGGPLEI   DSATDDDRSRNL DLDR ALSR LDETQQSWLL GPG+Q  K
Sbjct: 1   MSMYGRDPWGGPLEI-CHDSATDDDRSRNL-DLDRGALSRTLDETQQSWLLAGPGDQGRK 60

Query: 61  KKKYVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHK 120
           KKKYVDLGC++VSRK+FVWTVG LL +   AGL+  I K +PR              L K
Sbjct: 61  KKKYVDLGCLVVSRKLFVWTVGVLLAAAVFAGLVAGIAKAIPRXXXXXXXXXXXXXXLRK 120

Query: 121 ALMFFNAQRSGKLPKHNNVSWRGNSCTQDG-DDSSLFKDLSGGYYDAGDAIKFNFPASFA 180
           ALMFFNAQ+SGKLPK+NNV WRGNSC +DG  D ++ + L GGYYDAGDA+KFNFPA+F+
Sbjct: 121 ALMFFNAQKSGKLPKNNNVHWRGNSCMKDGLSDPAVGRSLVGGYYDAGDAVKFNFPAAFS 180

Query: 181 MTMLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSG 240
           MT+LSWSVIEYSAKYEA GEL H++D IKWG+DYFLKTFN TAD+I  + MQVG G TS 
Sbjct: 181 MTLLSWSVIEYSAKYEAVGELGHIRDTIKWGADYFLKTFNSTADTIDRVVMQVGSGATSP 240

Query: 241 GNTSPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHG 300
           G+T PNDHYCWMRPEDIDY RPV+ C +CSDLAAEMAA+LA+ASIVFKDNKAYSQKLVHG
Sbjct: 241 GSTQPNDHYCWMRPEDIDYPRPVVECHACSDLAAEMAASLAAASIVFKDNKAYSQKLVHG 300

Query: 301 ARTLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGI 360
           A TLFKFAR  RGRYS G S+A+ FYNSTSYWDEFVWGG+W+Y ATGNSSYLQL+T P +
Sbjct: 301 ATTLFKFARQNRGRYSAGGSDAAKFYNSTSYWDEFVWGGSWMYLATGNSSYLQLATHPKL 360

Query: 361 AKHAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSY 420
           AKHAGA+WGGPDYGV SWDNKL GAQVLLSRLRLFLSPGYPYEEILRTFHNQTSI+MCSY
Sbjct: 361 AKHAGAYWGGPDYGVFSWDNKLTGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIIMCSY 420

Query: 421 LPFFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTD 480
           LP F  FNRT+GGLIQLNHGRPQPLQYVVNAAFLA+LY DYLEAADTPGWYCGP+FY  +
Sbjct: 421 LPIFKSFNRTKGGLIQLNHGRPQPLQYVVNAAFLASLYGDYLEAADTPGWYCGPHFYPIE 480

Query: 481 VLRDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDT 540
            LR+FA+TQI+YILGKNP KMSY+VG+GN YPK VHHRGASIPKN + Y CKGGWKWR+T
Sbjct: 481 TLRNFARTQIEYILGKNPLKMSYVVGYGNRYPKRVHHRGASIPKNGVHYGCKGGWKWRET 540

Query: 541 KKPNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKN 600
           KKPNPN +VGAMVAGPD+HDGF DVR NYNYTE TLAGNAGLVAALVALSGE   G+DKN
Sbjct: 541 KKPNPNIIVGAMVAGPDRHDGFKDVRKNYNYTEATLAGNAGLVAALVALSGE-GHGVDKN 600

Query: 601 TIFSAVPPMFPTXXXXXAPWKP 619
           T+FSAVPPMFP+XXXXXAPWKP
Sbjct: 601 TMFSAVPPMFPSXXXXXAPWKP 619

BLAST of Carg25859 vs. Swiss-Prot
Match: sp|Q84R49|GUN10_ORYSJ (Endoglucanase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU2 PE=2 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 2.6e-277
Identity = 457/623 (73.35%), Postives = 522/623 (83.79%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLL-GPGEQ---KK 62
           M+GRDPWGGPLEI+ ADSATDDDRSR   DLDR AL R LDETQQSWLL GPG+Q   KK
Sbjct: 1   MFGRDPWGGPLEISNADSATDDDRSR---DLDRGALMRQLDETQQSWLLAGPGDQAGKKK 60

Query: 63  KKYVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKA 122
           KKYVDLGC+++ RKIF+WTVGT+L  G   G + +IVK VP           YT ALHKA
Sbjct: 61  KKYVDLGCMVLDRKIFMWTVGTILGVGLFIGFVMMIVKLVPHXXXXXXXXXXYTQALHKA 120

Query: 123 LMFFNAQRSGKLPKHNNVSWRGNSCTQDG-DDSSLFKDLSGGYYDAGDAIKFNFPASFAM 182
           LMFFNAQRSG LPKHN VSWRGNSC +DG  DS++ K L GG+YDAGDAIKFN+P +++M
Sbjct: 121 LMFFNAQRSGPLPKHNGVSWRGNSCMKDGLSDSTVRKSLVGGFYDAGDAIKFNYPMAWSM 180

Query: 183 TMLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGG 242
           TMLSWSVIEY AKYEA GEL+HVK++IKWG+DY LKTFN +AD+I  I  QVGVGDTS G
Sbjct: 181 TMLSWSVIEYKAKYEAIGELDHVKELIKWGTDYLLKTFNSSADTIDRIVAQVGVGDTSKG 240

Query: 243 NTSPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGA 302
              PNDHYCWMRPEDIDY RPV  C SCSDLA+EMAAALA+ASIVFKD+K YS KLV GA
Sbjct: 241 GAQPNDHYCWMRPEDIDYPRPVTECHSCSDLASEMAAALAAASIVFKDSKTYSDKLVRGA 300

Query: 303 RTLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIA 362
           + L+KF R QRGRYS   S+ +IFYNSTSYWDEFVWGGAW+Y+ATGN++YL ++T PG+A
Sbjct: 301 KALYKFGRLQRGRYSPNGSDQAIFYNSTSYWDEFVWGGAWMYFATGNNTYLSVATAPGMA 360

Query: 363 KHAGAFW-GGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSY 422
           KHAGA+W   P+YGV +WD+KL GAQVLLSRLRLFLSPGYPYEEILRTFHNQT  VMCSY
Sbjct: 361 KHAGAYWLDSPNYGVFTWDDKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTDNVMCSY 420

Query: 423 LPFFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTD 482
           LP +  FN T+GG+IQLNHGRPQPLQYVVNAAFLA+LYSDYL+AADTPGWYCGP FY+T+
Sbjct: 421 LPMYNSFNFTKGGMIQLNHGRPQPLQYVVNAAFLASLYSDYLDAADTPGWYCGPTFYTTE 480

Query: 483 VLRDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDT 542
           VLR FA++Q+DY+LGKNP KMSY+VGFGN YPK  HHRGASIP N +KY CKGG+KWR+T
Sbjct: 481 VLRKFARSQLDYVLGKNPLKMSYVVGFGNKYPKRAHHRGASIPHNGVKYGCKGGFKWRET 540

Query: 543 KKPNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSG-EKTSGIDK 602
           KKPNPN ++GA+VAGPD+HDGF DVRTNYNYTEPTLA NAGLVAAL++L+     SGIDK
Sbjct: 541 KKPNPNILIGALVAGPDRHDGFKDVRTNYNYTEPTLAANAGLVAALISLTNIHVKSGIDK 600

Query: 603 NTIFSAVPPMFPTXXXXXAPWKP 619
           NTIFSAVPPMFPT  XX + WKP
Sbjct: 601 NTIFSAVPPMFPTPPXXPSAWKP 620

BLAST of Carg25859 vs. Swiss-Prot
Match: sp|Q9STW8|GUN21_ARATH (Endoglucanase 21 OS=Arabidopsis thaliana OX=3702 GN=KOR3 PE=2 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 1.5e-264
Identity = 445/621 (71.66%), Postives = 507/621 (81.64%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 62
           MYGRDPWGGPLEIN ADS TDDDRSRNLQDLDRA  SRPLDETQQSWLLGP   KKKKYV
Sbjct: 1   MYGRDPWGGPLEINAADSMTDDDRSRNLQDLDRATPSRPLDETQQSWLLGPKVLKKKKYV 60

Query: 63  DLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALMFF 122
           DLGCI+VSRKIF+WT+GT++V+  L+G ITLIVKT+           NYT+AL  AL FF
Sbjct: 61  DLGCILVSRKIFLWTLGTIVVTALLSGFITLIVKTLXXXXXXXXXXXNYTIALRTALKFF 120

Query: 123 NAQRSGKLPKH-NNVSWRGNSCTQD--GDDSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 182
           NAQ+SGKLPK+  NVSWR +SC QD  GD    +KDL GGYYDAGD+IKFNFP S+AMTM
Sbjct: 121 NAQQSGKLPKNIYNVSWRHDSCLQDGKGDPGQCYKDLVGGYYDAGDSIKFNFPMSYAMTM 180

Query: 183 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNT 242
           LSWSVIEYSAKY+AAGEL HVK++IKWG+DYFLKTFN +AD+I  +  QVG G +  G+ 
Sbjct: 181 LSWSVIEYSAKYQAAGELEHVKELIKWGTDYFLKTFNSSADNIYVMVEQVGSGVSGRGSE 240

Query: 243 SPNDHYCWMRPEDIDYVRPVITC-SSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAR 302
             NDHYCWMRPEDI Y R V  C SSCSDLAAEMAAALASASIVFKDN+ YS+ LVHGA+
Sbjct: 241 LHNDHYCWMRPEDIHYKRTVSQCYSSCSDLAAEMAAALASASIVFKDNRLYSKNLVHGAK 300

Query: 303 TLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAK 362
           TL++FA   R RYS    E+S FYNS+ + DE +WGGAWLYYATGN +YL+  T+  +A+
Sbjct: 301 TLYRFATTSRNRYSQNGKESSKFYNSSMFEDELLWGGAWLYYATGNVTYLERVTSHHMAE 360

Query: 363 HAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLP 422
            AGAF   P YGV SWDNKL GAQ+LL+R+RLFLSPGYPYE++L  FHNQT  VMCSYLP
Sbjct: 361 KAGAFGNSPYYGVFSWDNKLPGAQLLLTRMRLFLSPGYPYEDMLSEFHNQTGRVMCSYLP 420

Query: 423 FFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVL 482
           ++ KFNRT GGLIQLNHG PQPLQYV NAAFLA L+SDYLEAADTPGWYCGPNFY+T+ L
Sbjct: 421 YYKKFNRTNGGLIQLNHGAPQPLQYVANAAFLAALFSDYLEAADTPGWYCGPNFYTTEFL 480

Query: 483 RDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKK 542
           R+F+++QIDYILGKNPRKMSY+VG+G  YPK VHHRGASIPKN +K  C GG+KW+ +KK
Sbjct: 481 RNFSRSQIDYILGKNPRKMSYVVGYGQRYPKQVHHRGASIPKN-MKETCTGGFKWKKSKK 540

Query: 543 PNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEK-TSGIDKNT 602
            NPN + GAMVAGPDKHDGFHD+RTNYNYTEPTLAGNAGLVAALVALSGEK   GIDKNT
Sbjct: 541 NNPNAINGAMVAGPDKHDGFHDIRTNYNYTEPTLAGNAGLVAALVALSGEKAVGGIDKNT 600

Query: 603 IFSAVPPMFPTXXXXXAPWKP 619
           +FSAVPP+        APW P
Sbjct: 601 MFSAVPPLVMATPPPPAPWTP 620

BLAST of Carg25859 vs. Swiss-Prot
Match: sp|Q7XUK4|GUN12_ORYSJ (Endoglucanase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU3 PE=2 SV=2)

HSP 1 Score: 762.3 bits (1967), Expect = 4.2e-219
Identity = 373/627 (59.49%), Postives = 460/627 (73.37%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALS---RPLDETQQSWLLGPGEQKKK 62
           MY  + WGG  EI  AD A +DD SRN+ DLDR ALS     LDETQQSWLLGP E KKK
Sbjct: 1   MYSANHWGGSFEI-AADGAAEDDHSRNM-DLDRGALSARQHQLDETQQSWLLGPPEAKKK 60

Query: 63  -KYVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKA 122
            KYVDLGC++V RK+  W + TLL +  L GL  +I K++P+     PPPD YT ALHKA
Sbjct: 61  DKYVDLGCVVVKRKLLWWVLWTLLAAFILIGLPVIIAKSIPKKKPHAPPPDQYTDALHKA 120

Query: 123 LMFFNAQRSGKLPKHNNVSWRGNSCTQDGDDSSLFK-DLSGGYYDAGDAIKFNFPASFAM 182
           L+FFNAQ+SG+LPK+N + WRGNS   DG D +  K  L GGYYDAGD IKF+FP +F+M
Sbjct: 121 LLFFNAQKSGRLPKNNGIKWRGNSGLSDGSDLTDVKGGLVGGYYDAGDNIKFHFPLAFSM 180

Query: 183 TMLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGG 242
           TMLSWSVIEYSAKY+A GE +HV+++IKWG+DY L TFN +A +I  +  QVG+   +G 
Sbjct: 181 TMLSWSVIEYSAKYKAVGEYDHVRELIKWGTDYLLLTFNSSASTIDKVYSQVGIAKING- 240

Query: 243 NTSPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGA 302
            T P+DHYCW RPED+ Y RPV T  S  DL  EMAAALA+ASIVF+DN AYS+KLV+GA
Sbjct: 241 -TQPDDHYCWNRPEDMAYPRPVQTAGSAPDLGGEMAAALAAASIVFRDNAAYSKKLVNGA 300

Query: 303 RTLFKFARD--QRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPG 362
             ++KFAR   +R  YS GN     +YNSTSYWDE++W  AW+YYATGN++Y+  +T P 
Sbjct: 301 AAVYKFARSSGRRTPYSRGNQYIEYYYNSTSYWDEYMWSAAWMYYATGNNTYITFATDPR 360

Query: 363 IAKHAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCS 422
           + K+A AF+   D+ V SWDNKL GA++LLSRLR+FL+PGYPYEE L  +HN TS+ MC+
Sbjct: 361 LPKNAKAFYSILDFSVFSWDNKLPGAELLLSRLRMFLNPGYPYEESLIGYHNTTSMNMCT 420

Query: 423 YLPFFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYST 482
           Y P F  FN T+GGL Q NHG+ QPLQY V  +FLA LY+DY+E+ + PGWYCGP F + 
Sbjct: 421 YFPRFGAFNFTKGGLAQFNHGKGQPLQYTVANSFLAALYADYMESVNVPGWYCGPYFMTV 480

Query: 483 DVLRDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRD 542
           D LR FA++Q++YILG NP+KMSY+VG+G  YP+ +HHRGAS P N IKY+C GG+KWRD
Sbjct: 481 DDLRSFARSQVNYILGDNPKKMSYVVGYGKKYPRRLHHRGASTPHNGIKYSCTGGYKWRD 540

Query: 543 TKKPNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALS----GEKTS 602
           TK  +PN +VGAMV GPDK+D F D R  Y   EPTL GNAGLVAALVAL+    G   +
Sbjct: 541 TKGADPNVLVGAMVGGPDKNDQFKDARLTYAQNEPTLVGNAGLVAALVALTNSGRGAGVT 600

Query: 603 GIDKNTIFSAVPPMFPTXXXXXAPWKP 619
            +DKNT+FSAVPPMFP      + WKP
Sbjct: 601 AVDKNTMFSAVPPMFPATPPPPSKWKP 623

BLAST of Carg25859 vs. TrEMBL
Match: tr|Q0KIX2|Q0KIX2_CUCME (Endoglucanase OS=Cucumis melo OX=3656 GN=CmEGase1 PE=2 SV=1)

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 599/622 (96.30%), Postives = 610/622 (98.07%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEIN ADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINAADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGD-DSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 180
           FFNAQRSGKLPKHNNVSWRGNSCT+DGD  SSLFKDLSGGYYDAGDAIKFNFPASFAMTM
Sbjct: 121 FFNAQRSGKLPKHNNVSWRGNSCTRDGDGSSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 180

Query: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNT 240
           LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSI TI MQVGVGDTSGGNT
Sbjct: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSISTIVMQVGVGDTSGGNT 240

Query: 241 SPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300
           SPNDHYCWMRPEDIDYVRPV+TCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART
Sbjct: 241 SPNDHYCWMRPEDIDYVRPVLTCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300

Query: 301 LFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 360
           LFKFAR+QRGRYS GN+EA+IFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH
Sbjct: 301 LFKFAREQRGRYSAGNAEAAIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 360

Query: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420
           AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF
Sbjct: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420

Query: 421 FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR 480
           FTKFNRTRGGLIQLNHGRPQPLQY+VNAAFLATLYSDYLEAADTPGWYCGPNFYST+VLR
Sbjct: 421 FTKFNRTRGGLIQLNHGRPQPLQYIVNAAFLATLYSDYLEAADTPGWYCGPNFYSTEVLR 480

Query: 481 DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKP 540
           DFAKTQIDYILGKNPRKMSY+VGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDT KP
Sbjct: 481 DFAKTQIDYILGKNPRKMSYVVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTTKP 540

Query: 541 NPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSG---EKTSGIDKN 600
           NPNT+VGAMVAGPD+ DGFHDVRTNYNYTEPTLAGNAGLVAALVALSG   EK +GIDKN
Sbjct: 541 NPNTLVGAMVAGPDRRDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGENSEKATGIDKN 600

Query: 601 TIFSAVPPMFPTXXXXXAPWKP 619
           TIFSAVPPMFPTXXXXXAPWKP
Sbjct: 601 TIFSAVPPMFPTXXXXXAPWKP 622

BLAST of Carg25859 vs. TrEMBL
Match: tr|A0A0A0L858|A0A0A0L858_CUCSA (Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G113920 PE=3 SV=1)

HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 596/622 (95.82%), Postives = 607/622 (97.59%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEIN ADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINAADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDGD-DSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 180
           FFNAQRSGKLPKHNNVSWRGNSCT+DGD  SSLFKDLSGGYYDAGDAIKFNFPASFAMTM
Sbjct: 121 FFNAQRSGKLPKHNNVSWRGNSCTRDGDGSSSLFKDLSGGYYDAGDAIKFNFPASFAMTM 180

Query: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNT 240
           LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSI TI MQVGVGDTSGGNT
Sbjct: 181 LSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSISTIVMQVGVGDTSGGNT 240

Query: 241 SPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300
           SPNDHYCWMRPEDIDYVRPV+TCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART
Sbjct: 241 SPNDHYCWMRPEDIDYVRPVLTCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGART 300

Query: 301 LFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 360
           LF FAR QRGRYS GN+EA+IFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH
Sbjct: 301 LFDFARKQRGRYSAGNAEAAIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKH 360

Query: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420
           AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF
Sbjct: 361 AGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPF 420

Query: 421 FTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLR 480
           F+KFN TRGGLIQLNHGRPQPLQY+VNAAFLATLYSDYLEAADTPGWYCGPNFYST+VLR
Sbjct: 421 FSKFNHTRGGLIQLNHGRPQPLQYIVNAAFLATLYSDYLEAADTPGWYCGPNFYSTEVLR 480

Query: 481 DFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKP 540
           DFAKTQIDYILGKNPRKMSY+VGFGNHYPKHVHHRGASIPKNK+KYNCKGGWKWRDT KP
Sbjct: 481 DFAKTQIDYILGKNPRKMSYVVGFGNHYPKHVHHRGASIPKNKVKYNCKGGWKWRDTTKP 540

Query: 541 NPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSG---EKTSGIDKN 600
           NPNT+VGAMVAGPDK DGFHDVRTNYNYTEPTLAGNAGLVAALVALSG   EK +GIDKN
Sbjct: 541 NPNTLVGAMVAGPDKRDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGENSEKATGIDKN 600

Query: 601 TIFSAVPPMFPTXXXXXAPWKP 619
           TIFSAVPPMFPTXXXXXAPWKP
Sbjct: 601 TIFSAVPPMFPTXXXXXAPWKP 622

BLAST of Carg25859 vs. TrEMBL
Match: tr|A0A2I4EJ10|A0A2I4EJ10_9ROSI (Endoglucanase OS=Juglans regia OX=51240 GN=LOC108990004 PE=3 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 567/620 (91.45%), Postives = 595/620 (95.97%), Query Frame = 0

Query: 1   MSMYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60
           MSMYGRDPWGGPLEIN ADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK
Sbjct: 1   MSMYGRDPWGGPLEINAADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKK 60

Query: 61  YVDLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALM 120
           YVDLGCIIVSRKIFVWTVGTLLVS FLAG ITLIVKTVPRHHH HPPPDNYTLALHKALM
Sbjct: 61  YVDLGCIIVSRKIFVWTVGTLLVSAFLAGFITLIVKTVPRHHHSHPPPDNYTLALHKALM 120

Query: 121 FFNAQRSGKLPKHNNVSWRGNSCTQDG--DDSSLFKDLSGGYYDAGDAIKFNFPASFAMT 180
           FFNAQRSGKLPKHNNVSWRG+SC  DG  D ++ +KDL GGYYDAGDAIKFNFPASFAMT
Sbjct: 121 FFNAQRSGKLPKHNNVSWRGSSCLDDGKSDSATFYKDLVGGYYDAGDAIKFNFPASFAMT 180

Query: 181 MLSWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGN 240
           MLSWSVIEYSA+YEAAGELNHVK+IIKWG+DY LKTFN++AD+IG IA QVGVGDTSGG+
Sbjct: 181 MLSWSVIEYSARYEAAGELNHVKEIIKWGTDYLLKTFNNSADTIGRIAAQVGVGDTSGGS 240

Query: 241 TSPNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAR 300
           T+PNDHYCWMRPEDIDY RPV  CSSCSDLAAEMAAALA+ASIVFKDNKAYSQKLVHGAR
Sbjct: 241 TTPNDHYCWMRPEDIDYKRPVSECSSCSDLAAEMAAALAAASIVFKDNKAYSQKLVHGAR 300

Query: 301 TLFKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAK 360
           TLFKF+RDQRGRYS   SEA++FYNSTSYWDEFVWGGAWLYYATGNSSYLQL+TTPG+AK
Sbjct: 301 TLFKFSRDQRGRYSASGSEAAMFYNSTSYWDEFVWGGAWLYYATGNSSYLQLATTPGLAK 360

Query: 361 HAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLP 420
           HAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYE+ILRTFHNQT+I+MCSYLP
Sbjct: 361 HAGAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEDILRTFHNQTNIIMCSYLP 420

Query: 421 FFTKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVL 480
            FT FNRT+GGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYST VL
Sbjct: 421 IFTSFNRTKGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTKVL 480

Query: 481 RDFAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKK 540
           RDFAKTQ+DYILGKNPRKMSY+VGFGNHYP+HVHHRGASIPKNKIKYNCKGGWKWRD+ K
Sbjct: 481 RDFAKTQVDYILGKNPRKMSYVVGFGNHYPRHVHHRGASIPKNKIKYNCKGGWKWRDSTK 540

Query: 541 PNPNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTI 600
           PNPNT+VGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSG+ TSGIDKNTI
Sbjct: 541 PNPNTLVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGDGTSGIDKNTI 600

Query: 601 FSAVPPMFPTXXXXXAPWKP 619
           FSAVPPMFPTXXXXXAPWKP
Sbjct: 601 FSAVPPMFPTXXXXXAPWKP 620

BLAST of Carg25859 vs. TrEMBL
Match: tr|A0A1R3HIW3|A0A1R3HIW3_COCAP (Endoglucanase OS=Corchorus capsularis OX=210143 GN=CCACVL1_19041 PE=3 SV=1)

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 559/618 (90.45%), Postives = 588/618 (95.15%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 62
           MYGRDPWGGPLEIN ADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV
Sbjct: 1   MYGRDPWGGPLEINAADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 60

Query: 63  DLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALMFF 122
           DLGCIIVSRKIFVWTVGT+LV+G LAG ITLIVKTVPRHHH HPPPDNYTLALHKALMFF
Sbjct: 61  DLGCIIVSRKIFVWTVGTILVAGVLAGFITLIVKTVPRHHHRHPPPDNYTLALHKALMFF 120

Query: 123 NAQRSGKLPKHNNVSWRGNSCTQDG--DDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 182
           NAQRSGKLPKHNNVSWRGNSC QDG  D S L KDL GG+YDAGDAIKFNFPA+++MT+L
Sbjct: 121 NAQRSGKLPKHNNVSWRGNSCMQDGKSDPSVLMKDLVGGFYDAGDAIKFNFPAAYSMTLL 180

Query: 183 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 242
           SWSVIEYSAK+EAAGELNH+K+IIKWG+DYFLKTFNHTAD+I  +A QVGVGDTSGG+TS
Sbjct: 181 SWSVIEYSAKFEAAGELNHIKEIIKWGTDYFLKTFNHTADTIDRVAAQVGVGDTSGGSTS 240

Query: 243 PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 302
           PNDHYCWMRPEDIDY RPV  C SCSDLAAEMAAALASASIVFKDNKAYSQKLVHGA TL
Sbjct: 241 PNDHYCWMRPEDIDYPRPVYECHSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAATL 300

Query: 303 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 362
           FKFARDQRGRYS G S+ASIFYNS+SYWDEFVWGGAW+YYATGN SYLQL+T P +AKHA
Sbjct: 301 FKFARDQRGRYSAGGSDASIFYNSSSYWDEFVWGGAWMYYATGNQSYLQLATHPKLAKHA 360

Query: 363 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 422
           GAFWGGPDYGV SWDNKL GAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCS+LP F
Sbjct: 361 GAFWGGPDYGVFSWDNKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSFLPVF 420

Query: 423 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 482
           T FNRTRGG+IQLNHGRPQPLQYVVNAAFLATL+SDYL+AADTPGWYCGPNF+STDVLRD
Sbjct: 421 TSFNRTRGGMIQLNHGRPQPLQYVVNAAFLATLFSDYLDAADTPGWYCGPNFFSTDVLRD 480

Query: 483 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 542
           FAKTQIDYILGKNPRKMSY+VGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDT KPN
Sbjct: 481 FAKTQIDYILGKNPRKMSYVVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTSKPN 540

Query: 543 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 602
           PNT+VGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSG+K++GIDKNTIFS
Sbjct: 541 PNTLVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGDKSTGIDKNTIFS 600

Query: 603 AVPPMFPTXXXXXAPWKP 619
           AVPPMFPTXXXXX PWKP
Sbjct: 601 AVPPMFPTXXXXXXPWKP 618

BLAST of Carg25859 vs. TrEMBL
Match: tr|A0A1R3H5L3|A0A1R3H5L3_9ROSI (Endoglucanase OS=Corchorus olitorius OX=93759 GN=COLO4_31102 PE=3 SV=1)

HSP 1 Score: 1175.6 bits (3040), Expect = 0.0e+00
Identity = 558/618 (90.29%), Postives = 588/618 (95.15%), Query Frame = 0

Query: 3   MYGRDPWGGPLEINTADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 62
           MYGRDPWGGPLEIN ADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV
Sbjct: 1   MYGRDPWGGPLEINAADSATDDDRSRNLQDLDRAALSRPLDETQQSWLLGPGEQKKKKYV 60

Query: 63  DLGCIIVSRKIFVWTVGTLLVSGFLAGLITLIVKTVPRHHHPHPPPDNYTLALHKALMFF 122
           DLGCIIVSRKIFVWTVGT+LV+G LAG ITLIVKTVPRHHH HPPPDNYTLALHKALMFF
Sbjct: 61  DLGCIIVSRKIFVWTVGTILVAGVLAGFITLIVKTVPRHHHRHPPPDNYTLALHKALMFF 120

Query: 123 NAQRSGKLPKHNNVSWRGNSCTQDG--DDSSLFKDLSGGYYDAGDAIKFNFPASFAMTML 182
           NAQRSGKLPKHNNVSWRGNSC QDG  D S L KDL GG+YDAGDAIKFNFPA+++MT+L
Sbjct: 121 NAQRSGKLPKHNNVSWRGNSCMQDGKSDPSVLMKDLVGGFYDAGDAIKFNFPAAYSMTLL 180

Query: 183 SWSVIEYSAKYEAAGELNHVKDIIKWGSDYFLKTFNHTADSIGTIAMQVGVGDTSGGNTS 242
           SWSVIEYSAK+EAAGEL+H+K+IIKWG+DYFLKTFNHTAD+I  +A QVGVGDTSGG+TS
Sbjct: 181 SWSVIEYSAKFEAAGELSHIKEIIKWGTDYFLKTFNHTADTIDRVAAQVGVGDTSGGSTS 240

Query: 243 PNDHYCWMRPEDIDYVRPVITCSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGARTL 302
           PNDHYCWMRPEDIDY RPV  C SCSDLAAEMAAALASASIVFKDNKAYSQKLVHGA TL
Sbjct: 241 PNDHYCWMRPEDIDYPRPVYECHSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAATL 300

Query: 303 FKFARDQRGRYSGGNSEASIFYNSTSYWDEFVWGGAWLYYATGNSSYLQLSTTPGIAKHA 362
           FKFARDQRGRYS G S+ASIFYNS+SYWDEFVWGGAW+YYATGN SYLQL+T P +AKHA
Sbjct: 301 FKFARDQRGRYSAGGSDASIFYNSSSYWDEFVWGGAWMYYATGNQSYLQLATHPKLAKHA 360

Query: 363 GAFWGGPDYGVLSWDNKLAGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSYLPFF 422
           GAFWGGPDYGV SWDNKL GAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCS+LP F
Sbjct: 361 GAFWGGPDYGVFSWDNKLPGAQVLLSRLRLFLSPGYPYEEILRTFHNQTSIVMCSFLPVF 420

Query: 423 TKFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLEAADTPGWYCGPNFYSTDVLRD 482
           T FNRTRGG+IQLNHGRPQPLQYVVNAAFLATL+SDYL+AADTPGWYCGPNF+STDVLRD
Sbjct: 421 TSFNRTRGGMIQLNHGRPQPLQYVVNAAFLATLFSDYLDAADTPGWYCGPNFFSTDVLRD 480

Query: 483 FAKTQIDYILGKNPRKMSYIVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTKKPN 542
           FAKTQIDYILGKNPRKMSY+VGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDT KPN
Sbjct: 481 FAKTQIDYILGKNPRKMSYVVGFGNHYPKHVHHRGASIPKNKIKYNCKGGWKWRDTSKPN 540

Query: 543 PNTVVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGEKTSGIDKNTIFS 602
           PNT+VGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSG+K++GIDKNTIFS
Sbjct: 541 PNTLVGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGDKSTGIDKNTIFS 600

Query: 603 AVPPMFPTXXXXXAPWKP 619
           AVPPMFPTXXXXX PWKP
Sbjct: 601 AVPPMFPTXXXXXXPWKP 618

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022942996.10.0e+00100.00endoglucanase 25-like [Cucurbita moschata][more]
XP_023542874.10.0e+0099.84endoglucanase 25-like [Cucurbita pepo subsp. pepo][more]
XP_022977523.10.0e+0099.51endoglucanase 25-like [Cucurbita maxima][more]
XP_022136360.10.0e+0096.45endoglucanase 25 [Momordica charantia][more]
NP_001284449.10.0e+0096.30endoglucanase 25-like [Cucumis melo] >BAF30815.1 endoglucanase [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G49720.10.0e+0082.93glycosyl hydrolase 9A1[more]
AT4G24260.18.1e-26671.66glycosyl hydrolase 9A3[more]
AT1G65610.12.6e-20354.63Six-hairpin glycosidases superfamily protein[more]
AT1G75680.11.2e-9643.38glycosyl hydrolase 9B7[more]
AT1G19940.11.8e-9541.75glycosyl hydrolase 9B5[more]
Match NameE-valueIdentityDescription
sp|Q38890|GUN25_ARATH0.0e+0082.93Endoglucanase 25 OS=Arabidopsis thaliana OX=3702 GN=KOR PE=1 SV=1[more]
sp|P0C1U4|GUN9_ORYSJ2.4e-29177.97Endoglucanase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU1 PE=2 SV=1[more]
sp|Q84R49|GUN10_ORYSJ2.6e-27773.35Endoglucanase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU2 PE=2 SV=1[more]
sp|Q9STW8|GUN21_ARATH1.5e-26471.66Endoglucanase 21 OS=Arabidopsis thaliana OX=3702 GN=KOR3 PE=2 SV=1[more]
sp|Q7XUK4|GUN12_ORYSJ4.2e-21959.49Endoglucanase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU3 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
tr|Q0KIX2|Q0KIX2_CUCME0.0e+0096.30Endoglucanase OS=Cucumis melo OX=3656 GN=CmEGase1 PE=2 SV=1[more]
tr|A0A0A0L858|A0A0A0L858_CUCSA0.0e+0095.82Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G113920 PE=3 SV=1[more]
tr|A0A2I4EJ10|A0A2I4EJ10_9ROSI0.0e+0091.45Endoglucanase OS=Juglans regia OX=51240 GN=LOC108990004 PE=3 SV=1[more]
tr|A0A1R3HIW3|A0A1R3HIW3_COCAP0.0e+0090.45Endoglucanase OS=Corchorus capsularis OX=210143 GN=CCACVL1_19041 PE=3 SV=1[more]
tr|A0A1R3H5L3|A0A1R3H5L3_9ROSI0.0e+0090.29Endoglucanase OS=Corchorus olitorius OX=93759 GN=COLO4_31102 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR0089286-hairpin_glycosidase_sf
IPR033126Glyco_hydro_9_Asp/Glu_AS
IPR001701Glyco_hydro_9
IPR0123416hp_glycosidase-like_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25859-RACarg25859-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012341Six-hairpin glycosidase-like superfamilyGENE3DG3DSA:1.50.10.10coord: 108..597
e-value: 7.9E-148
score: 495.3
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 111..582
e-value: 4.6E-125
score: 418.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 28..609
NoneNo IPR availablePANTHERPTHR22298:SF36ENDOGLUCANASE 21-RELATEDcoord: 28..609
IPR033126Glycosyl hydrolases family 9, Asp/Glu active sitesPROSITEPS00698GLYCOSYL_HYDROL_F9_2coord: 558..576
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILYSSF48208Six-hairpin glycosidasescoord: 108..601