Carg25839 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25839
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	uroporphyrinogen-III synthase, chloroplastic
Location	Cucurbita_argyrosperma_scaffold_356 : 114569 .. 115206 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCACGAATCTGTTGCAACAGCGCATGGATCAATCTTATTTCAGAGCCAAAGGAGTGGGAAAATTCACTTGCCTGTATTGGGGAAACAACTGCAGCAGTAGCAAAAAGATTAGGATTGAAAAACGTTTACTACCCGAAGAATCCTGGTCTTGAAGGGTGAGTTTTTCCATACTTCTACCTTACAATTTAGTAGTTGAGTTCCTGGTAACCACCTACCTAGAAATTTTTTAGCAACTAAATCTAGTAGGGTCGGACTATTATACTTTAGATTAGTCGAGATGCACCTAAGCTGATTCAGACACTCACGGATATAATAATTAATAATAAATATAACAATAAGATCAAATAGACTAAATGTGAATTTACAAACGTGATAATTGAAATGCAGGTGGGTTGATAGTATATTGGAAGCAGTAAGAGCAGAAGCACAGGTCGTTTAGGACATTGTCCAGACTAGGAAGTCATCTTTACTTCTTTTCCAGAGTTGCAGACGGAGACGGAACAAAGCATTCAACCATTGTATCATCACATGGCATGATCACAGTCGATATGCCCGAGTTTCTATTCGAGGTTAGTCACTTTTCCAGTAGTTTTACGTTCATTTATCTGTCTGTCCAGTTCGATATCAGATAA

mRNA sequence

ATGGATCCACGAATCTGTTGCAACAGCGCATGGATCAATCTTATTTCAGAGCCAAAGGAGTGGGAAAATTCACTTGCCTGTATTGGGGAAACAACTGCAGCAGTAGCAAAAAGATTAGGATTGAAAAACGTTTACTACCCGAAGAATCCTGGTCTTGAAGGAGTTGCAGACGGAGACGGAACAAAGCATTCAACCATTGTATCATCACATGGCATGATCACAGTCGATATGCCCGAGTTTCTATTCGAGTTCGATATCAGATAA

Coding sequence (CDS)

ATGGATCCACGAATCTGTTGCAACAGCGCATGGATCAATCTTATTTCAGAGCCAAAGGAGTGGGAAAATTCACTTGCCTGTATTGGGGAAACAACTGCAGCAGTAGCAAAAAGATTAGGATTGAAAAACGTTTACTACCCGAAGAATCCTGGTCTTGAAGGAGTTGCAGACGGAGACGGAACAAAGCATTCAACCATTGTATCATCACATGGCATGATCACAGTCGATATGCCCGAGTTTCTATTCGAGTTCGATATCAGATAA

Protein sequence

MDPRICCNSAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVADGDGTKHSTIVSSHGMITVDMPEFLFEFDIR

Homology

[Close]

BLAST of Carg25839 vs. NCBI nr
Match: XP_022951382.1 (uroporphyrinogen-III synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 100.5 bits (249), Expect = 2.9e-18
Identity = 46/48 (95.83%), Postives = 46/48 (95.83%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEG  D
Sbjct: 247 AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGWVD 294

[Close]

BLAST of Carg25839 vs. NCBI nr
Match: XP_023002006.1 (uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 99.0 bits (245), Expect = 8.5e-18
Identity = 45/48 (93.75%), Postives = 46/48 (95.83%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGL+G  D
Sbjct: 246 AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLKGWVD 293

[Close]

BLAST of Carg25839 vs. NCBI nr
Match: XP_023537362.1 (uroporphyrinogen-III synthase, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-17
Identity = 45/48 (93.75%), Postives = 45/48 (93.75%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNP LEG  D
Sbjct: 175 AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVD 222

[Close]

BLAST of Carg25839 vs. NCBI nr
Match: XP_023537361.1 (uroporphyrinogen-III synthase, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-17
Identity = 45/48 (93.75%), Postives = 45/48 (93.75%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNP LEG  D
Sbjct: 247 AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVD 294

[Close]

BLAST of Carg25839 vs. NCBI nr
Match: XP_022962039.1 (uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita moschata] >XP_022962040.1 uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 95.9 bits (237), Expect = 7.2e-17
Identity = 42/48 (87.50%), Postives = 44/48 (91.67%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AW+NLISEPKEWE SLACIGETTAA AKRLGLKN+YYPKNPGLEG  D
Sbjct: 260 AWVNLISEPKEWEKSLACIGETTAAAAKRLGLKNIYYPKNPGLEGWVD 307

[Close]

BLAST of Carg25839 vs. TAIR10
Match: AT2G26540.1 (uroporphyrinogen-III synthase family protein)

HSP 1 Score: 74.3 bits (181), Expect = 4.1e-14
Identity = 30/45 (66.67%), Postives = 38/45 (84.44%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEG 55
           AW++LI   ++W N +ACIGETTA+ A+RLGLKNVYYP+ PGLEG
Sbjct: 255 AWLHLIQNEEQWSNYVACIGETTASAARRLGLKNVYYPEKPGLEG 299

[Close]

BLAST of Carg25839 vs. Swiss-Prot
Match: sp|O48721|HEM4_ARATH (Uroporphyrinogen-III synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=UROS PE=2 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 7.3e-13
Identity = 30/45 (66.67%), Postives = 38/45 (84.44%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEG 55
           AW++LI   ++W N +ACIGETTA+ A+RLGLKNVYYP+ PGLEG
Sbjct: 255 AWLHLIQNEEQWSNYVACIGETTASAARRLGLKNVYYPEKPGLEG 299

[Close]

BLAST of Carg25839 vs. Swiss-Prot
Match: sp|Q10QR9|HEM4_ORYSJ (Uroporphyrinogen-III synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=UROS PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.8e-11
Identity = 27/45 (60.00%), Postives = 36/45 (80.00%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEG 55
           AW+NL S+   W N++ACIGETTA+ AK+ GLK++YYP  PGL+G
Sbjct: 237 AWLNLASQVDNWGNAIACIGETTASAAKKFGLKSIYYPTTPGLDG 281

[Close]

BLAST of Carg25839 vs. TrEMBL
Match: tr|A0A0A0LLB2|A0A0A0LLB2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360600 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.9e-16
Identity = 40/48 (83.33%), Postives = 43/48 (89.58%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AW+NLIS+PKEW+ SLACIGETTAA A RLGLKNVYYPKNPGLEG  D
Sbjct: 246 AWVNLISDPKEWDKSLACIGETTAAAANRLGLKNVYYPKNPGLEGWVD 293

[Close]

BLAST of Carg25839 vs. TrEMBL
Match: tr|A0A1S3BBN0|A0A1S3BBN0_CUCME (uroporphyrinogen-III synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487917 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.6e-15
Identity = 39/48 (81.25%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AW+NLI +PKEW+ SLACIGETTAA A RLGLKNVYYPKNPGLEG  D
Sbjct: 247 AWVNLIPDPKEWDKSLACIGETTAAAANRLGLKNVYYPKNPGLEGWVD 294

[Close]

BLAST of Carg25839 vs. TrEMBL
Match: tr|M0S608|M0S608_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis OX=214687 GN=103976089 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 8.4e-14
Identity = 36/48 (75.00%), Postives = 40/48 (83.33%), Query Frame = 0


Query: 10  AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           AW+ LI EP+ W+NS+ACIGETT   AKRLGLKNVYYPKNPGLEG  D
Sbjct: 236 AWVKLIREPENWDNSVACIGETTGLAAKRLGLKNVYYPKNPGLEGWVD 283

[Close]

BLAST of Carg25839 vs. TrEMBL
Match: tr|W9RIJ9|W9RIJ9_9ROSA (Uroporphyrinogen-III synthase OS=Morus notabilis OX=981085 GN=L484_026091 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.9e-13
Identity = 34/48 (70.83%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 10 AWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
          AW+NL+ +P++W NS+ACIGETTA+ AKRLGL+NVYYP NPGLEG  D
Sbjct: 33 AWVNLLPKPEQWRNSVACIGETTASAAKRLGLRNVYYPTNPGLEGWVD 80

[Close]

BLAST of Carg25839 vs. TrEMBL
Match: tr|A0A2H5NH05|A0A2H5NH05_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_044530 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.4e-13
Identity = 35/49 (71.43%), Postives = 43/49 (87.76%), Query Frame = 0


Query: 9   SAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGVAD 58
           S+W+NLIS+ ++W NS+ACIGETTA+ AKRLGLKNVYYP +PGLEG  D
Sbjct: 242 SSWVNLISDTEQWSNSVACIGETTASAAKRLGLKNVYYPTHPGLEGWVD 290

The following BLAST results are available for this feature:

BLAST of Carg25839 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951382.1	2.9e-18	95.83	uroporphyrinogen-III synthase, chloroplastic [Cucurbita moschata]	[more]
XP_023002006.1	8.5e-18	93.75	uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita maxima]	[more]
XP_023537362.1	3.2e-17	93.75	uroporphyrinogen-III synthase, chloroplastic isoform X2 [Cucurbita pepo subsp. p...	[more]
XP_023537361.1	3.2e-17	93.75	uroporphyrinogen-III synthase, chloroplastic isoform X1 [Cucurbita pepo subsp. p...	[more]
XP_022962039.1	7.2e-17	87.50	uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita moschata] >XP_02296...	[more]

BLAST of Carg25839 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G26540.1	4.1e-14	66.67	uroporphyrinogen-III synthase family protein	[more]

BLAST of Carg25839 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O48721\|HEM4_ARATH	7.3e-13	66.67	Uroporphyrinogen-III synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
sp\|Q10QR9\|HEM4_ORYSJ	1.8e-11	60.00	Uroporphyrinogen-III synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=...	[more]

BLAST of Carg25839 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LLB2\|A0A0A0LLB2_CUCSA	6.9e-16	83.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360600 PE=4 SV=1	[more]
tr\|A0A1S3BBN0\|A0A1S3BBN0_CUCME	2.6e-15	81.25	uroporphyrinogen-III synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
tr\|M0S608\|M0S608_MUSAM	8.4e-14	75.00	Uncharacterized protein OS=Musa acuminata subsp. malaccensis OX=214687 GN=103976...	[more]
tr\|W9RIJ9\|W9RIJ9_9ROSA	1.9e-13	70.83	Uroporphyrinogen-III synthase OS=Morus notabilis OX=981085 GN=L484_026091 PE=4 S...	[more]
tr\|A0A2H5NH05\|A0A2H5NH05_CITUN	2.4e-13	71.43	Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_044530 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004852	uroporphyrinogen-III synthase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0033014	tetrapyrrole biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR036108	4pyrrol_syn_uPrphyn_synt_sf
IPR003754	4pyrrol_synth_uPrphyn_synth

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015994	chlorophyll metabolic process
biological_process	GO:0006783	heme biosynthetic process
biological_process	GO:0033014	tetrapyrrole biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004852	uroporphyrinogen-III synthase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25839-RA	Carg25839-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	PFAM	PF02602	HEM4	coord: 12..55 e-value: 7.8E-7 score: 28.6
None	No IPR available	GENE3D	G3DSA:3.40.50.10090		coord: 8..60 e-value: 2.2E-5 score: 25.9
None	No IPR available	PANTHER	PTHR45501	FAMILY NOT NAMED	coord: 10..57
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	SUPERFAMILY	SSF69618	HemD-like	coord: 22..57

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg25839	Silver-seed gourd	carcarB215
Carg25839	Cucumber (Gy14) v2	carcgybB0286
Carg25839	Cucumber (Gy14) v1	carcgyB0322
Carg25839	Cucurbita maxima (Rimu)	carcmaB0377
Carg25839	Cucurbita maxima (Rimu)	carcmaB0378
Carg25839	Cucurbita moschata (Rifu)	carcmoB0374
Carg25839	Cucurbita moschata (Rifu)	carcmoB0375
Carg25839	Cucurbita pepo (Zucchini)	carcpeB0382
Carg25839	Cucurbita pepo (Zucchini)	carcpeB0383
Carg25839	Wild cucumber (PI 183967)	carcpiB0303
Carg25839	Cucumber (Chinese Long) v2	carcuB0299
Carg25839	Cucumber (Chinese Long) v3	carcucB0302
Carg25839	Bottle gourd (USVL1VR-Ls)	carlsiB245
Carg25839	Melon (DHL92) v3.5.1	carmeB0274
Carg25839	Melon (DHL92) v3.6.1	carmedB0286
Carg25839	Watermelon (Charleston Gray)	carwcgB0264
Carg25839	Watermelon (97103) v1	carwmB0263
Carg25839	Watermelon (97103) v2	carwmbB0280
Carg25839	Wax gourd	carwgoB0356