BLAST of Carg25768 vs. NCBI nr
Match:
XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])
HSP 1 Score: 694.1 bits (1790), Expect = 2.3e-196
Identity = 334/340 (98.24%), Postives = 334/340 (98.24%), Query Frame = 0
Query: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLST DRPLLVRKILDAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60
Query: 61 QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120
QEFGFFQVINHGV RRVLEETTRIFKKFHAMSGAEK KECSRDP KSCRVYTSSENYRKE
Sbjct: 61 QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
GYLGGEMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 340
BLAST of Carg25768 vs. NCBI nr
Match:
XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 691.8 bits (1784), Expect = 1.2e-195
Identity = 331/338 (97.93%), Postives = 333/338 (98.52%), Query Frame = 0
Query: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMAN IPVLDLSTRDRPLLVRKILDAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
Query: 61 QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120
QEFGFFQVINHGV +RVLEETTRIFKKFHAMSGAEKAKECSRDP KSCRVYTSSENYRKE
Sbjct: 61 QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYC+ MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338
BLAST of Carg25768 vs. NCBI nr
Match:
XP_023004374.1 (hyoscyamine 6-dioxygenase-like [Cucurbita maxima])
HSP 1 Score: 686.4 bits (1770), Expect = 4.9e-194
Identity = 328/337 (97.33%), Postives = 333/337 (98.81%), Query Frame = 0
Query: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
MGEVKFVSNWEGVQSVPPDY+YPPEKRPGNVVVPMANAIPV+DLSTRDRPLLVRKILDAS
Sbjct: 1 MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60
Query: 61 QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120
QEFGFFQVINHGV RRVLEETTRIFKKFHAMSGAEKAKECSRDP KSCRVYTSSENYRKE
Sbjct: 61 QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120
Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180
QTHCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYC+AMRTLVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180
Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
GYL EMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSD 338
NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGS+
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSN 337
BLAST of Carg25768 vs. NCBI nr
Match:
XP_004146985.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_7G378370 [Cucumis sativus])
HSP 1 Score: 609.4 bits (1570), Expect = 7.6e-171
Identity = 285/337 (84.57%), Postives = 311/337 (92.28%), Query Frame = 0
Query: 2 GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQ 61
G KFVSNWE V+SVP YVYPPEKRPGN+VVPMA AIPV+DLS RDR LLVRKILDAS+
Sbjct: 4 GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
Query: 62 EFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQ 121
EFGFFQ+INHGV ++V EET RIFK+FHAMSG EKAKECS+DP +SCRVYTSSENY KEQ
Sbjct: 64 EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183
Query: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
Y GGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
PIDNAFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340
BLAST of Carg25768 vs. NCBI nr
Match:
XP_008451261.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])
HSP 1 Score: 605.1 bits (1559), Expect = 1.4e-169
Identity = 284/337 (84.27%), Postives = 310/337 (91.99%), Query Frame = 0
Query: 2 GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQ 61
G+ KFVSNWE +SVP YVYPPEKRPGNVVVPMA AIPV+DLS DR LLVRKIL AS+
Sbjct: 4 GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63
Query: 62 EFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQ 121
E GFFQ+INHGV +RV E T RIFK+FHAMSG EKAKECS+DP +SCRVYTSSENY+KEQ
Sbjct: 64 ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123
Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
HCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183
Query: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
YLGGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
PID AFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE TMEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303
Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340
BLAST of Carg25768 vs. TAIR10
Match:
AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 255.0 bits (650), Expect = 6.6e-68
Identity = 137/334 (41.02%), Postives = 198/334 (59.28%), Query Frame = 0
Query: 5 KFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQEFG 64
K +S ++P +YV P RP V P++DLS+ DR L+++I A FG
Sbjct: 4 KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63
Query: 65 FFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQTHC 124
FFQVINHGV +++++E + ++F +MS EK K S DP K+ R+ TS N +KE+ +
Sbjct: 64 FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123
Query: 125 WRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLG 184
WRD L +C+P+ KYV+ WP NPP ++E+V Y +R + +I EL+SE LGL + Y+
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183
Query: 185 GEMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
+ E M VN+YPPCP P LT GL H DP+ +TIL Q+ V GLQ+L DGQW V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243
Query: 245 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL-- 304
P +AFV+NIG LQ +SNG K+ HRAVTN + R S+ + P D M PAK L
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303
Query: 305 -INEANPPRYRALKFKDFQRNYLPRASDTKAVME 333
++ P Y+ + ++ + + R D + +E
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336
BLAST of Carg25768 vs. TAIR10
Match:
AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 232.3 bits (591), Expect = 4.6e-61
Identity = 129/336 (38.39%), Postives = 194/336 (57.74%), Query Frame = 0
Query: 13 VQSVPPDYVYPPEKRPG-NVVVPMANAIPVLDLST---RDRPLLVRKILDASQEFGFFQV 72
V VP +YV P RP + V ++IP++DL +R ++ + A GFFQ+
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74
Query: 73 INHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQTHCWRDA 132
NHGVP +++ ++F S +E+ K S D KK+ R+ TS N KE+ WRD
Sbjct: 75 KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134
Query: 133 LIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGEMS 192
L +C+P++ +++ WP P +REV Y ++R LVL +LE +SE LGL + + +
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194
Query: 193 EN-PLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNA 252
++ M +N+YP CP P LT GL H D +LIT+L Q EV+GLQV KDG+WI V+P+ N
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254
Query: 253 FVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA--N 312
F+VN+G +QVISN K K+ HRAV N+ + R S+ P ++ + PA+ LINE +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314
Query: 313 PPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
P YR + ++ + A DT++ CI S + S
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES---CIDSFKAS 346
BLAST of Carg25768 vs. TAIR10
Match:
AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 223.8 bits (569), Expect = 1.6e-58
Identity = 121/333 (36.34%), Postives = 190/333 (57.06%), Query Frame = 0
Query: 7 VSNWEGVQSVPPDYVYPPEKRPG-NVVVPMANAIPVLDLST---RDRPLLVRKILDASQE 66
VS++ +P +YV P RP + V ++IP++DL +R ++V+++ A
Sbjct: 11 VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70
Query: 67 FGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQT 126
+GFFQ+ NHGVP + + + ++F +E+ K S DP K+ R+ TS N ++
Sbjct: 71 YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130
Query: 127 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGY 186
WRD L +C P++ ++ WP +P +REV Y ++R LVL +LE +SE LGL +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190
Query: 187 LGGEMSENPL-MLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGV 246
+ + ++ M N+YPPCP P LT GL H DP++IT+L Q +V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250
Query: 247 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 306
PI N F+VNIG +QVISN K K+ HRAV N + R S+ +P + + PA L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310
Query: 307 NEANP-PRYRALKFKDFQRNYLPRASDTKAVME 333
NE + YR F ++ + R+ T + ++
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCLD 342
BLAST of Carg25768 vs. TAIR10
Match:
AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 206.1 bits (523), Expect = 3.5e-53
Identity = 119/318 (37.42%), Postives = 171/318 (53.77%), Query Frame = 0
Query: 12 GVQSVPPDYVYPPEKRP--GNVVVPMANAIPVLDLSTRDRPLL----VRKILDASQEFGF 71
GV VP YV PP +RP G+ + +PV+DLS +P L + +I A +EFGF
Sbjct: 23 GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82
Query: 72 FQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQTHCW 131
FQVINHG+P V+ + +F + EK S + + R Y +S N+ ++ H W
Sbjct: 83 FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142
Query: 132 RDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGG 191
RD + HPL K++ WP NPP Y++ VG Y A L +++E +SE LGL + YL
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202
Query: 192 EMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLK-DGQWIGVHPI 251
E+ E + +M VN YP CP P + LG+ H D S +TIL Q GLQ++ + W+ V I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262
Query: 252 DNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA 311
+ A +V +G ++V+SNG K+ HR N ++ R S L + PA L+N
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322
Query: 312 NPPRYRALKFKDFQRNYL 322
N P Y F DF NY+
Sbjct: 323 NAPAYGEFSFNDF-LNYI 338
BLAST of Carg25768 vs. TAIR10
Match:
AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 192.2 bits (487), Expect = 5.2e-49
Identity = 116/325 (35.69%), Postives = 168/325 (51.69%), Query Frame = 0
Query: 12 GVQSVPPDYVYPPEKRP--GNVVVPMANAIPVLDLSTRDRPLL----VRKILDASQEFGF 71
G VP Y+ PP +RP G + +PV+DLS PLL + +I A + FGF
Sbjct: 23 GDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLHDPLLRLCVIHEIELACKGFGF 82
Query: 72 FQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQTHCW 131
FQVINHG+ V+++ +F + EK S + ++ R Y +S N+ ++ H W
Sbjct: 83 FQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR-YGTSINHSTDRVHYW 142
Query: 132 RDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGG 191
RD + HPL +++ WP NPP Y+E VG Y A L +++E +SE LGL + YL
Sbjct: 143 RDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISESLGLEKNYLQE 202
Query: 192 EMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLK-DGQWIGVHPI 251
E+ E + +M VN YP CP P + LG+ H D +TIL Q GLQ+ + W+ V I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKDCNNNWVCVPYI 262
Query: 252 DNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA 311
+ A +V +G ++V+SNG K+ HR N R S L + PA L+NE
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKISPATQLVNEN 322
Query: 312 NPPRYRALKFKDFQRNYLPRASDTK 329
P Y F DF +Y+ R T+
Sbjct: 323 KPAAYGEFSFNDF-LDYISRNDITQ 345
BLAST of Carg25768 vs. Swiss-Prot
Match:
sp|Q9FLV0|DMR6_ARATH (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)
HSP 1 Score: 255.0 bits (650), Expect = 1.2e-66
Identity = 137/334 (41.02%), Postives = 198/334 (59.28%), Query Frame = 0
Query: 5 KFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQEFG 64
K +S ++P +YV P RP V P++DLS+ DR L+++I A FG
Sbjct: 4 KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63
Query: 65 FFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQTHC 124
FFQVINHGV +++++E + ++F +MS EK K S DP K+ R+ TS N +KE+ +
Sbjct: 64 FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123
Query: 125 WRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLG 184
WRD L +C+P+ KYV+ WP NPP ++E+V Y +R + +I EL+SE LGL + Y+
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183
Query: 185 GEMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
+ E M VN+YPPCP P LT GL H DP+ +TIL Q+ V GLQ+L DGQW V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243
Query: 245 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL-- 304
P +AFV+NIG LQ +SNG K+ HRAVTN + R S+ + P D M PAK L
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303
Query: 305 -INEANPPRYRALKFKDFQRNYLPRASDTKAVME 333
++ P Y+ + ++ + + R D + +E
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336
BLAST of Carg25768 vs. Swiss-Prot
Match:
sp|P24397|HY6H_HYONI (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)
HSP 1 Score: 252.7 bits (644), Expect = 5.9e-66
Identity = 139/335 (41.49%), Postives = 193/335 (57.61%), Query Frame = 0
Query: 6 FVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQEFGF 65
FVSNW +SV ++ P +KR VP+ N +P++DL + LLV++I A Q+FG
Sbjct: 4 FVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQ-QHHHLLVQQITKACQDFGL 63
Query: 66 FQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVY-----------TSS 125
FQVINHG P ++ ET + K+F A+ EK K PK +
Sbjct: 64 FQVINHGFPEELMLETMEVCKEFFALPAEEKEK---FKPKGEAAKFXXXXXXXXXXXXXX 123
Query: 126 ENYRKEQTHCWRDALIFNCHPLDK-YVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVS 185
E+ W+D L CHPLD+ V+ WP+ P KYREVV Y + +R L + +L+ +
Sbjct: 124 XQLSNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYIC 183
Query: 186 EGLGLGRGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQ--V 245
EGLGL GY E+S+ +ML N+YPPCP+PS TLG H D +LIT+L Q++ GLQ +
Sbjct: 184 EGLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLI 243
Query: 246 LKDGQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEV 305
+KD WI V PI AFVVN+G L+VI+N K + + HR VT+ R S+ L+ P
Sbjct: 244 VKDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSC 303
Query: 306 TMEPAKCLINEANPPRYRALKFKDFQRNYLPRASD 327
T+EPAK L+N+ NPP Y+ + +F YL SD
Sbjct: 304 TIEPAKELLNQDNPPLYKPYSYSEFADIYLSDKSD 332
BLAST of Carg25768 vs. Swiss-Prot
Match:
sp|Q8W2X5|FL3H2_ORYSJ (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)
HSP 1 Score: 251.5 bits (641), Expect = 1.3e-65
Identity = 131/321 (40.81%), Postives = 187/321 (58.26%), Query Frame = 0
Query: 15 SVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQEFGFFQVINHGVP 74
++P YV P +RP +V IPV+DL++ DR +V + DA + GFFQV+NHG+
Sbjct: 19 TMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGID 78
Query: 75 RRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQTHCWRDALIFNCH 134
++ + ++F + EKAK S DP K R+ TS N RKE H WRD L +C+
Sbjct: 79 AALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHCY 138
Query: 135 PLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGEMSENPL-M 194
PL ++V WP NPP ++E++G YC +R L + E +SE LGL GY+ + E M
Sbjct: 139 PLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQHM 198
Query: 195 LVNHYPPCPNPSLTLGLSQHCDPSLITILF--QEVNGLQVLKDGQWIGVHPIDNAFVVNI 254
VN+YP CP P LT GL H DP+ +TIL +V GLQVL DG+WI V+P A V+NI
Sbjct: 199 AVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINI 258
Query: 255 GFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRYRAL 314
G LQ +SNGK ++ HRAV N+ R S+ + P + V + PAK LI + +P YR
Sbjct: 259 GDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRNY 318
Query: 315 KFKDFQRNYLPRASDTKAVME 333
+ ++ + + R D + +E
Sbjct: 319 TYDEYYKKFWSRNLDQEHCLE 338
BLAST of Carg25768 vs. Swiss-Prot
Match:
sp|Q9ZSA7|DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)
HSP 1 Score: 232.3 bits (591), Expect = 8.2e-60
Identity = 129/336 (38.39%), Postives = 194/336 (57.74%), Query Frame = 0
Query: 13 VQSVPPDYVYPPEKRPG-NVVVPMANAIPVLDLST---RDRPLLVRKILDASQEFGFFQV 72
V VP +YV P RP + V ++IP++DL +R ++ + A GFFQ+
Sbjct: 15 VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74
Query: 73 INHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQTHCWRDA 132
NHGVP +++ ++F S +E+ K S D KK+ R+ TS N KE+ WRD
Sbjct: 75 KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134
Query: 133 LIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGYLGGEMS 192
L +C+P++ +++ WP P +REV Y ++R LVL +LE +SE LGL + + +
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194
Query: 193 EN-PLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNA 252
++ M +N+YP CP P LT GL H D +LIT+L Q EV+GLQV KDG+WI V+P+ N
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254
Query: 253 FVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA--N 312
F+VN+G +QVISN K K+ HRAV N+ + R S+ P ++ + PA+ LINE +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314
Query: 313 PPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRRS 341
P YR + ++ + A DT++ CI S + S
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES---CIDSFKAS 346
BLAST of Carg25768 vs. Swiss-Prot
Match:
sp|Q9ZSA8|DLO1_ARATH (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)
HSP 1 Score: 223.8 bits (569), Expect = 2.9e-57
Identity = 121/333 (36.34%), Postives = 190/333 (57.06%), Query Frame = 0
Query: 7 VSNWEGVQSVPPDYVYPPEKRPG-NVVVPMANAIPVLDLST---RDRPLLVRKILDASQE 66
VS++ +P +YV P RP + V ++IP++DL +R ++V+++ A
Sbjct: 11 VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70
Query: 67 FGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQT 126
+GFFQ+ NHGVP + + + ++F +E+ K S DP K+ R+ TS N ++
Sbjct: 71 YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130
Query: 127 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRGY 186
WRD L +C P++ ++ WP +P +REV Y ++R LVL +LE +SE LGL +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190
Query: 187 LGGEMSENPL-MLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGV 246
+ + ++ M N+YPPCP P LT GL H DP++IT+L Q +V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250
Query: 247 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 306
PI N F+VNIG +QVISN K K+ HRAV N + R S+ +P + + PA L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310
Query: 307 NEANP-PRYRALKFKDFQRNYLPRASDTKAVME 333
NE + YR F ++ + R+ T + ++
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCLD 342
BLAST of Carg25768 vs. TrEMBL
Match:
tr|A0A0A0K5H3|A0A0A0K5H3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)
HSP 1 Score: 609.4 bits (1570), Expect = 5.0e-171
Identity = 285/337 (84.57%), Postives = 311/337 (92.28%), Query Frame = 0
Query: 2 GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQ 61
G KFVSNWE V+SVP YVYPPEKRPGN+VVPMA AIPV+DLS RDR LLVRKILDAS+
Sbjct: 4 GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
Query: 62 EFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQ 121
EFGFFQ+INHGV ++V EET RIFK+FHAMSG EKAKECS+DP +SCRVYTSSENY KEQ
Sbjct: 64 EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183
Query: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
Y GGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
PIDNAFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340
BLAST of Carg25768 vs. TrEMBL
Match:
tr|A0A1S3BR20|A0A1S3BR20_CUCME (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)
HSP 1 Score: 605.1 bits (1559), Expect = 9.4e-170
Identity = 284/337 (84.27%), Postives = 310/337 (91.99%), Query Frame = 0
Query: 2 GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQ 61
G+ KFVSNWE +SVP YVYPPEKRPGNVVVPMA AIPV+DLS DR LLVRKIL AS+
Sbjct: 4 GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63
Query: 62 EFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKEQ 121
E GFFQ+INHGV +RV E T RIFK+FHAMSG EKAKECS+DP +SCRVYTSSENY+KEQ
Sbjct: 64 ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123
Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181
HCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183
Query: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
YLGGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
PID AFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE TMEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303
Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340
BLAST of Carg25768 vs. TrEMBL
Match:
tr|A0A1S3BRU2|A0A1S3BRU2_CUCME (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492607 PE=3 SV=1)
HSP 1 Score: 441.4 bits (1134), Expect = 1.8e-120
Identity = 207/336 (61.61%), Postives = 261/336 (77.68%), Query Frame = 0
Query: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
M + KFVSNW+GV+SVP YVYPPEKR G V + IPV+D + DR LL++K+ D +
Sbjct: 2 MSQTKFVSNWKGVESVPETYVYPPEKRFG---VSVKKTIPVIDWAIDDRTLLLKKVHDVT 61
Query: 61 QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDP-KKSCRVYTSSENYRK 120
QEFGF QVINHGVP+ ++EET +I K+FHAMS +K +ECS+DP KSCRVY+SS+NY
Sbjct: 62 QEFGFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYET 121
Query: 121 EQTHCWRDALIFNCHP-LDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGL 180
EQ H W+D L CHP LD+ V FWPQNPPK+REVV AY + + LEILE++SEGLGL
Sbjct: 122 EQIHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGL 181
Query: 181 GRGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWI 240
+GYL GEMS+NP + V+HYPPCPNPSLT+GL+QH DP+LITILFQ+VNGLQ LKDGQWI
Sbjct: 182 EKGYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWI 241
Query: 241 GVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKC 300
V P+ NAF+VN+G+VL+VISNGK+KA EHR VTN SRQSL Y+VYP ++V +EP+KC
Sbjct: 242 DVDPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKC 301
Query: 301 LINEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 335
INEANPP Y +L+FKDF + + + D + ++ I
Sbjct: 302 FINEANPPHYLSLEFKDFIKKLMSKPPDRETALKFI 334
BLAST of Carg25768 vs. TrEMBL
Match:
tr|A0A0A0K5H0|A0A0A0K5H0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G375850 PE=3 SV=1)
HSP 1 Score: 439.9 bits (1130), Expect = 5.3e-120
Identity = 207/339 (61.06%), Postives = 257/339 (75.81%), Query Frame = 0
Query: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
M KFVSNW GV SVP YVYPPEKR G + + +IPV+D S DR LL++++ D +
Sbjct: 2 MSRTKFVSNWGGVVSVPETYVYPPEKRFG---ISVKKSIPVIDWSD-DRALLLKRVHDVT 61
Query: 61 QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120
QEFGF QVINHGVP+ ++EE +I K+FH MS +K +ECS+DP KSC+VYTSS+NY E
Sbjct: 62 QEFGFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENE 121
Query: 121 QTHCWRDALIFNCHP-LDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLG 180
Q H W+D CHP LDK + FWPQNPPK REVV AY + + EILE++SEGLGL
Sbjct: 122 QIHLWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLE 181
Query: 181 RGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIG 240
+GY GEMS+NP L +HYPPCPNPSLT+GLSQH DP+LITILFQ+VNGLQ LKDGQWI
Sbjct: 182 KGYFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWID 241
Query: 241 VHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL 300
V P+ +AF+VN+G+V +VISNGKLKAAEHR VTN+ SRQ+L Y+VYP+++VT+EP+KC
Sbjct: 242 VDPLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCF 301
Query: 301 INEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 339
INEANPP YR+L+FKDF + + D K ME I D+
Sbjct: 302 INEANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFISFDQ 336
BLAST of Carg25768 vs. TrEMBL
Match:
tr|A0A0A0K745|A0A0A0K745_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G375860 PE=3 SV=1)
HSP 1 Score: 429.9 bits (1104), Expect = 5.4e-117
Identity = 201/335 (60.00%), Postives = 255/335 (76.12%), Query Frame = 0
Query: 1 MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60
M + KFVSNW+G+ SVP YV PPEKR G V + IPV+DL+T DR LL +K+LD S
Sbjct: 1 MSQTKFVSNWKGIGSVPETYVCPPEKRVG---VSVKKKIPVIDLATEDRDLLSKKVLDVS 60
Query: 61 QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120
QEFGFFQVINHGV + ++EET R+ ++FHAMS +K ECS+DP KSC +Y+S+ NY KE
Sbjct: 61 QEFGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKE 120
Query: 121 QTHCWRDALIFNCHP-LDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLG 180
+ H W+D+L C+P L+K V FWPQNPPK+REV+ AY + M+ LEILE++SEGLGL
Sbjct: 121 ELHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLE 180
Query: 181 RGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIG 240
+ Y G MS NP LV+HYPPCPNPSLTLGL++H DP++ITILFQ+VNGLQ LKDG+WI
Sbjct: 181 KEYFSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWID 240
Query: 241 VHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL 300
V P+ +AF+VN+G+V +VISNGK+KAAEHR VTN SRQSL Y VYP++ +T+EP+KC
Sbjct: 241 VDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCF 300
Query: 301 INEANPPRYRALKFKDFQRNYLPRASDTKAVMECI 335
INEANPP YR L+FKDF + D + M+ I
Sbjct: 301 INEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFI 332
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022959666.1 | 2.3e-196 | 98.24 | hyoscyamine 6-dioxygenase-like [Cucurbita moschata] | [more] |
| XP_023514953.1 | 1.2e-195 | 97.93 | hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_023004374.1 | 4.9e-194 | 97.33 | hyoscyamine 6-dioxygenase-like [Cucurbita maxima] | [more] |
| XP_004146985.1 | 7.6e-171 | 84.57 | PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus] >KGN44748.1 hypothet... | [more] |
| XP_008451261.1 | 1.4e-169 | 84.27 | PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G24530.1 | 6.6e-68 | 41.02 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10490.1 | 4.6e-61 | 38.39 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT4G10500.1 | 1.6e-58 | 36.34 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT2G44800.1 | 3.5e-53 | 37.42 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
| AT3G60290.1 | 5.2e-49 | 35.69 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
