Carg25522 (gene) Silver-seed gourd

BLAST of Carg25522 vs. NCBI nr
Match: XP_023548556.1 (GPI transamidase component PIG-T isoform X1 [Cucurbita pepo subsp. pepo] >XP_023548557.1 GPI transamidase component PIG-T isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1331.6 bits (3445), Expect = 0.0e+00
Identity = 667/669 (99.70%), Postives = 668/669 (99.85%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS
Sbjct: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL
Sbjct: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGF PASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFRPASENMRYGTLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
           AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIP+T
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPNT 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
           KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600

Query: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
           EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660

Query: 661 AAAWQYYFG 670
           AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669

BLAST of Carg25522 vs. NCBI nr
Match: XP_022953346.1 (GPI transamidase component GPI16 [Cucurbita moschata])

HSP 1 Score: 1329.7 bits (3440), Expect = 0.0e+00
Identity = 666/669 (99.55%), Postives = 667/669 (99.70%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQS APSS
Sbjct: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSGAPSS 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL
Sbjct: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAV+SAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVNSAIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLA SSNVYIQLDKGLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLASSSNVYIQLDKGLM 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
           AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
           KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600

Query: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
           EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660

Query: 661 AAAWQYYFG 670
           AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669

BLAST of Carg25522 vs. NCBI nr
Match: XP_022991212.1 (GPI transamidase component GPI16 [Cucurbita maxima])

HSP 1 Score: 1321.2 bits (3418), Expect = 0.0e+00
Identity = 662/669 (98.95%), Postives = 664/669 (99.25%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           M SFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQS APSS
Sbjct: 1   MVSFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSVAPSS 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRW YDRWGGFDSISSSNAKPSGVEL
Sbjct: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWHYDRWGGFDSISSSNAKPSGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGF PASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFRPASENMRYGTLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
           AELQGMLGEQEMLAVTRSSSEGS RNLAFELSANPDRVHMEMSSR+DKHLSVLYMFSVAE
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSTRNLAFELSANPDRVHMEMSSRNDKHLSVLYMFSVAE 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIP+T
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPNT 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
           KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600

Query: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
           EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660

Query: 661 AAAWQYYFG 670
           AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669

BLAST of Carg25522 vs. NCBI nr
Match: XP_008458020.1 (PREDICTED: GPI transamidase component PIG-T [Cucumis melo])

HSP 1 Score: 1216.8 bits (3147), Expect = 0.0e+00
Identity = 604/669 (90.28%), Postives = 633/669 (94.62%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MASFLRLISLLCLPILFAV  IGSVSEG EEDFSEDLLLKPLPDRKVLAHFHFQS APSS
Sbjct: 1   MASFLRLISLLCLPILFAVSVIGSVSEGAEEDFSEDLLLKPLPDRKVLAHFHFQSTAPSS 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL
Sbjct: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDVPPNEVDA+WKNLTHSLSGLFCASINFLESSTSYSAPKW FHPASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDATWKNLTHSLSGLFCASINFLESSTSYSAPKWSFHPASENMRYGTLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKG+YHSQRLHLLS+EFDS+ VDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGYYHSQRLHLLSSEFDSDGVDSAIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           DQTLTVVLQP+SHRG LGYS+A QLQPSWSLSSIFGR+V G+C LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPHSHRGTLGYSSATQLQPSWSLSSIFGRRVIGKCSLARSSNVYIQLDRGLM 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
           AELQG+LGEQE  A+ R+SSEGSI N AFELSANPDRVHMEMS+  DKH+SVLYMF VA 
Sbjct: 301 AELQGILGEQEKFAIARASSEGSISNPAFELSANPDRVHMEMSNGYDKHISVLYMFMVAA 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           KYD+SEPLDLRFTWKIP+ WSIPQAPL+VTRF+LGSGNERG IALQLKSTKP DQL+PDT
Sbjct: 361 KYDDSEPLDLRFTWKIPIAWSIPQAPLYVTRFLLGSGNERGVIALQLKSTKPRDQLMPDT 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           V  DSCSLLV+VFQVVPWYIKVYYHTLQVFIDDQ H ITNVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VFADSCSLLVRVFQVVPWYIKVYYHTLQVFIDDQRHIITNVVEKMQVSPSKDKVSPGVME 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDFFTSTQF E NSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFFTSTQFVEKNSS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
           KSPIL KWQG++PILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILLKWQGKNPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600

Query: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
           EEERFMK KA +K +VS LVSKLLAKLRGR WEPSESQ+ SS SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKSKATRKLRVSHLVSKLLAKLRGRQWEPSESQSHSSVSNSKSKLLFKVILVAGL 660

Query: 661 AAAWQYYFG 670
           AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669

BLAST of Carg25522 vs. NCBI nr
Match: XP_011659188.1 (PREDICTED: GPI transamidase component PIG-T [Cucumis sativus] >KGN44599.1 hypothetical protein Csa_7G340200 [Cucumis sativus])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 600/669 (89.69%), Postives = 629/669 (94.02%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MASFLRLISLLCLPILFAV   GSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS
Sbjct: 1   MASFLRLISLLCLPILFAVSVNGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYD WGGFD ISSSNAKPSGVEL
Sbjct: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDHWGGFDLISSSNAKPSGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDV PNEVDA+WKNLTHSLSGLFCASINFLESSTSYSAPKW FHP SENMRYGTLPR
Sbjct: 121 WAVFDVLPNEVDATWKNLTHSLSGLFCASINFLESSTSYSAPKWSFHPESENMRYGTLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKG+YHSQRLHLLS+EFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGYYHSQRLHLLSSEFDSNAVDSAIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           DQTLTVVLQP++HRG LGYS A QLQPSWSLS+IFGR+V G+C LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPHNHRGTLGYSTATQLQPSWSLSTIFGRRVIGKCSLARSSNVYIQLDRGLM 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
           AELQGMLGEQEM ++ R+  EGS  N AFELSANPDRVHMEMSSR DKH SVLYMF VAE
Sbjct: 301 AELQGMLGEQEMFSIARAGFEGSRSNPAFELSANPDRVHMEMSSRYDKHASVLYMFMVAE 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           KYD+SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+PDT
Sbjct: 361 KYDDSEPLDLRFTWKIPVAWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           V  D+CSLLV+VFQVVPWYIKVYYHTL +FI+DQPH I NV+EKMQVSPS+DKVSPGV+E
Sbjct: 421 VFADNCSLLVRVFQVVPWYIKVYYHTLHIFINDQPHKINNVIEKMQVSPSKDKVSPGVME 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           MLLK PCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKLPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
           KSPIL KWQGQ+PILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILLKWQGQNPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600

Query: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
           EEERFMK KA +K +V  LVSKLLAKLRGR WEPSESQ+PSS SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKSKATRKLRVLHLVSKLLAKLRGRQWEPSESQSPSSVSNSKSKLLFKVILVAGL 660

Query: 661 AAAWQYYFG 670
           AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669

BLAST of Carg25522 vs. TAIR10
Match: AT3G07140.1 (GPI transamidase component Gpi16 subunit family protein)

HSP 1 Score: 782.3 bits (2019), Expect = 2.3e-226
Identity = 411/669 (61.43%), Postives = 503/669 (75.19%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MAS LR + LL +   F V AI   S+  EE FSE LLLKPLPDRKVLAHFHF++RAP  
Sbjct: 1   MASLLRSLILLLIVQSFLV-AIAFGSKEVEE-FSEALLLKPLPDRKVLAHFHFENRAPP- 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
            SNS+GRHHHLFPKAISQLV K+R+KEMELSFTQGRW ++ WGGFD +SS NAKP GVEL
Sbjct: 61  -SNSHGRHHHLFPKAISQLVQKFRVKEMELSFTQGRWNHEHWGGFDPLSSMNAKPVGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDVP ++VD SWKNLTH+LSGLFCASINFLESSTSY+AP WGF P S+ +RYG+LPR
Sbjct: 121 WAVFDVPQSQVDTSWKNLTHALSGLFCASINFLESSTSYAAPTWGFGPNSDKLRYGSLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDK G+S LM+RPS+Y+GFYHSQRLHL + E     + S IVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKDGISALMNRPSVYRGFYHSQRLHLSTVESGQEGLGSGIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           +QTLTVVLQP +       S  + +QPSWSLSS+FGR+V GRCVLA+SSNVY+Q      
Sbjct: 241 EQTLTVVLQPET------TSVESNMQPSWSLSSLFGRQVVGRCVLAKSSNVYLQ------ 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
             L+G+LG +     T   +    +N  FELS  P+RV  E         S L++F + +
Sbjct: 301 --LEGLLGYESKNVDTEIEAHQLWKNAEFELSLKPERVIRE-------SCSFLFIFDI-D 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           K  +SEP DL  TWK P  WS  QAPLH +RF++GSGNERGAIA+ LK+T+  ++L    
Sbjct: 361 KSSDSEPFDLGLTWKRPSKWSCQQAPLHSSRFLMGSGNERGAIAILLKATESQEKLSGRD 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           +    C++   +FQ+ PWYIKVYYHTLQ+F+D Q  + + V++K+ VSPS DKVS G++E
Sbjct: 421 LTNGQCTIKANIFQIFPWYIKVYYHTLQIFVDQQQKTDSEVLKKINVSPSTDKVSSGMME 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           M+L+ PC +KS A++IE+DKGFLHIDEYPPDA+QG DIPSA+I+FPD   S  F E   S
Sbjct: 481 MMLELPCEVKSVAISIEYDKGFLHIDEYPPDANQGFDIPSALISFPDHHASLDFQE-ELS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
            SP+LS  + +S + SYTEVLLVP TTPDFSMPYNVITITCT+ ALYFGSLLNVLRRR+G
Sbjct: 541 NSPLLSSLKEKSLVRSYTEVLLVPLTTPDFSMPYNVITITCTIFALYFGSLLNVLRRRIG 600

Query: 601 EEERFMKDKA-KKKFQVSQLVSKLLAKLRGRHWE-PSESQAPSSSSNSKSKLLFKVILVA 660
           EEERF+K +A KK   + QL+S++ AK+RGR  E PS S+A SS  +  SKL+ K+ILVA
Sbjct: 601 EEERFLKSQAGKKTGGLKQLLSRITAKIRGRPIEAPSSSEAESSVLS--SKLILKIILVA 640

Query: 661 GLAAAWQYY 668
           G AAAWQY+
Sbjct: 661 GAAAAWQYF 640

BLAST of Carg25522 vs. Swiss-Prot
Match: sp|Q969N2|PIGT_HUMAN (GPI transamidase component PIG-T OS=Homo sapiens OX=9606 GN=PIGT PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.2e-76
Identity = 202/635 (31.81%), Postives = 304/635 (47.87%), Query Frame = 0

Query: 10  LLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSSRSNSYGRHH 69
           L  L +L   P    ++E   +   E+L++ PLP   V A F F++R  S        H+
Sbjct: 7   LALLVLLLLGPGGWCLAEPPRDSLREELVITPLPSGDVAATFQFRTRWDSELQREGVSHY 66

Query: 70  HLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVELWAVFDVPPN 129
            LFPKA+ QL+ KY ++E+ LSFTQG WR   WG           PSG ELW  F     
Sbjct: 67  RLFPKALGQLISKYSLRELHLSFTQGFWRTRYWG-----PPFLQAPSGAELWVWFQDTVT 126

Query: 130 EVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHP---ASEN----MRYGTLPREA 189
           +VD SWK L++ LSG+FCAS+NF++ ST+   P   F P   A++     +RY  LPRE 
Sbjct: 127 DVDKSWKELSNVLSGIFCASLNFID-STNTVTPTASFKPLGLANDTDHYFLRYAVLPREV 186

Query: 190 VCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVLDQ 249
           VCTENLTPW KLLPC  K+GLSVL+    ++   YHSQ +H+     ++     +  L Q
Sbjct: 187 VCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSYHSQAVHIRPVCRNARCTSISWELRQ 246

Query: 250 TLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLMAE 309
           TL+VV   +            Q +  WSL  +F R +   C LA  S VY+         
Sbjct: 247 TLSVVFDAFI---------TGQGKKDWSLFRMFSRTLTEPCPLASESRVYVD-------- 306

Query: 310 LQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAEKY 369
                       +T  + +    N   E+   P   + ++     K  ++  +   A   
Sbjct: 307 ------------ITTYNQD----NETLEVHPPPTTTYQDVILGTRKTYAIYDLLDTA-MI 366

Query: 370 DESEPLDLRFTWKIPVVWSIPQAP-LHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDTV 429
           + S  L+++  WK P     P  P LH  R+V G G ++G ++  L +T P         
Sbjct: 367 NNSRNLNIQLKWKRPPENEAPPVPFLHAQRYVSGYGLQKGELSTLLYNTHPYR------- 426

Query: 430 ITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLEM 489
                +  V +   VPWY+++Y HTL +    +     N    +   P++D++ P +LEM
Sbjct: 427 -----AFPVLLLDTVPWYLRLYVHTLTITSKGK----ENKPSYIHYQPAQDRLQPHLLEM 486

Query: 490 LLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVIT--FPDFFTSTQFAENNS 549
           L++ P       ++I+F++  L   EY PD + G  +  +V++   P    +      + 
Sbjct: 487 LIQLPAN-SVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSMVAAKPV---DW 546

Query: 550 SKSPILSKWQGQSP-----ILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNV 609
            +SP+ +     S      +  YTE LLV   TPDFSMPYNVI +TCTV+A+ +GS  N+
Sbjct: 547 EESPLFNSLFPVSDGSNYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNL 574

Query: 610 LRRRVGEEERFMKDKAKKKFQVSQLVSKLLAKLRG 630
           L R    EE      AK+       ++ L+ + RG
Sbjct: 607 LTRTFHIEEPRTGGLAKR-------LANLIRRARG 574

BLAST of Carg25522 vs. Swiss-Prot
Match: sp|Q8BXQ2|PIGT_MOUSE (GPI transamidase component PIG-T OS=Mus musculus OX=10090 GN=Pigt PE=1 SV=2)

HSP 1 Score: 286.2 bits (731), Expect = 9.5e-76
Identity = 199/616 (32.31%), Postives = 304/616 (49.35%), Query Frame = 0

Query: 26  SEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSSRSNSYGRHHHLFPKAISQLVHKYRI 85
           +EG  +   E+L++ PLP   V A F F++R  S        H+ LFPKA+ QL+ KY +
Sbjct: 27  AEGPRDSLREELVITPLPSGDVAATFQFRTRWDSDLQREGVSHYRLFPKALGQLISKYSL 86

Query: 86  KEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVELWAVFDVPPNEVDASWKNLTHSLSGL 145
           +E+ LSFTQG WR   WG           PSG ELW  F     +VD SW+ L++ LSG+
Sbjct: 87  RELHLSFTQGFWRTRYWG-----PPFLQAPSGAELWVWFQDTVTDVDKSWRELSNVLSGI 146

Query: 146 FCASINFLESSTSYSAPKWGFHP---ASEN----MRYGTLPREAVCTENLTPWLKLLPCR 205
           FCAS+NF++ +T+   P   F P   A++     +RY  LPRE VCTENLTPW KLLPC 
Sbjct: 147 FCASLNFID-ATNTVTPTASFKPLGLANDTDDYFLRYAVLPREVVCTENLTPWKKLLPCS 206

Query: 206 DKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVLDQTLTVVLQPYSHRGALG 265
            K+GLSVL+    ++   YHSQ +H+     +++    +  L QTL+VV   +       
Sbjct: 207 SKAGLSVLLKADRLFHTSYHSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFI------ 266

Query: 266 YSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLMAELQGMLGEQEMLAVTRS 325
                Q +  WSL  +F R +   C LA  S VY+        ++ G   + E L     
Sbjct: 267 ---TGQGKKDWSLFRMFSRTLTEACPLASQSLVYV--------DITGYSQDNETL----- 326

Query: 326 SSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAEKYDESEPLDLRFTWKIPV 385
                      E+S  P   + ++     K  +V  +F  A   + S  L+++  WK P 
Sbjct: 327 -----------EVSPPPTSTYQDVILGTRKTYAVYDLFDTA-MINNSRNLNIQLKWKRPP 386

Query: 386 -VWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDTVITDSCSLLVKVFQVVP 445
              ++P   LH  R+V G G ++G ++  L ++ P              +  V +  VVP
Sbjct: 387 DNEALPVPFLHAQRYVSGYGLQKGELSTLLYNSHPYR------------AFPVLLLDVVP 446

Query: 446 WYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLEMLLKFPCGLKSAALTIE 505
           WY+++Y HTL +    +     N    +   P++D+  P +LEML++ P       ++I+
Sbjct: 447 WYLRLYVHTLTITSKGK----ENKPSYIHYQPAQDRQQPHLLEMLIQLPAN-SVTKVSIQ 506

Query: 506 FDKGFLHIDEYPPDAHQGLDIPSAVIT--FPDFFTSTQFA-ENNSSKSPILSKWQGQSPI 565
           F++  L   EY PD + G  +  +V++   P    +     E +   + +     G S  
Sbjct: 507 FERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPVDWEGSPLFNTLFPVSDGSSYF 566

Query: 566 LS-YTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVGEEERFMKDKAKKK 625
           +  YTE LLV   TPDFSMPYNVI +TCTV+A+ +GS  N+L R    EE        K 
Sbjct: 567 VRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEE-------PKS 578

Query: 626 FQVSQLVSKLLAKLRG 630
             +++ ++ L+ + RG
Sbjct: 627 GGLAKRLANLIRRARG 578

BLAST of Carg25522 vs. Swiss-Prot
Match: sp|P38875|GPI16_YEAST (GPI transamidase component GPI16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GPI16 PE=1 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 4.9e-48
Identity = 181/624 (29.01%), Postives = 286/624 (45.83%), Query Frame = 0

Query: 33  FSEDLLLKPLPDRKVLAHFHFQSRA----PSSRSNSYGRHHH--LFPKAISQLVHKYRIK 92
           + E L+LKPLP+  +L  F FQ ++    P+  S SY  + H   FP+AI  L+     +
Sbjct: 36  YDEALVLKPLPNNDLLLSFAFQLQSEPFDPAVSSMSYDAYEHYTTFPRAIPPLLESTATR 95

Query: 93  EMELSFTQGRWRYDRWGGFDSISSSNAKPSGVELWA-VFDVPPNEVDASWKNLTHSLSGL 152
           +  L FT+G W    WG     +   A  SGVELW+ V  +   +   +WK L++SLSGL
Sbjct: 96  QFHLRFTRGFWDALSWGQLPH-AGKEAGASGVELWSQVQAMDQEQAFHNWKKLSNSLSGL 155

Query: 153 FCASINFLESS----------TSYSAPKWGFHPASENMRYGTLPREAVCTENLTPWLKLL 212
           FC+S+NF++ S          +   AP +        MR  +LP E +CTENLTP++KLL
Sbjct: 156 FCSSLNFIDESRTTFPRRSYASDIGAPLFNSTEKLYLMR-ASLPNEPICTENLTPFIKLL 215

Query: 213 PCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVLDQTLTVVLQPYSHRG 272
           P R KSGL+ L+D   ++   ++S  L + +   +         +D  + +V    S   
Sbjct: 216 PTRGKSGLTSLLDGHKLFDSLWNSISLDIATICSEDEDALCHYEMDARIEMVTHVPS--- 275

Query: 273 ALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLMAELQGMLGEQEMLAV 332
           AL        +P      + G  +  RC   +  + Y        ++    L +      
Sbjct: 276 ALARGERPIPKP------LDGNTL--RCDTDKPFDSYQCFPLPEPSQTHFKLSQ----LF 335

Query: 333 TRSSSEGSI-RNLAFELSANPDR-VHMEMSSRDDKHLSVL-YMFSVAEKYDESEP----- 392
            R  + G++  N    + A  DR       S DD   S     F ++   +E        
Sbjct: 336 ARPINNGNLFANRPTRICAEVDRSTWTAFLSVDDTIFSTHDNCFDLSNDQNEGGSGYDFI 395

Query: 393 LDLRFTWKI-PVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDTVITDSC 452
           L+   T K+ P+V      P+HV+R + G+G +RG + +       +D   P  +I    
Sbjct: 396 LESTDTTKVTPIV----PVPIHVSRSLTGNGQDRGGMRIVFH----NDNDTPVKLI---- 455

Query: 453 SLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITN--VVEKMQVSPSEDKVSPGVLEMLLK 512
                 F+ +PW+++VY  +LQ+     P    N  +++K  +  + D+  PG LE  + 
Sbjct: 456 -----YFESLPWFMRVYLSSLQITSTTSPQLQENDIILDKYYLQ-AADRKRPGHLEFTML 515

Query: 513 FPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSSKSPI 572
            P       +T +FDK  L   EYPPDA+ G +I +AVIT                   +
Sbjct: 516 IPAN-TDIVMTYQFDKALLQFAEYPPDANHGFEIDAAVIT-------------------V 575

Query: 573 LSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRV--GEE 627
           LS     S     T  LL+  +TPDFSMPYNVI +T T++ L FG L N++ +R+   EE
Sbjct: 576 LSLESSSSLYEMRTSTLLLSLSTPDFSMPYNVIILTSTIMGLIFGMLYNLMVKRMVTVEE 604

BLAST of Carg25522 vs. Swiss-Prot
Match: sp|O94380|GPI16_SCHPO (GPI transamidase component PIG-T homolog OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpi16 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 5.1e-45
Identity = 170/634 (26.81%), Postives = 288/634 (45.43%), Query Frame = 0

Query: 12  CLPILFAVPAIG-SVSEGT-EEDFSEDLLLKPLPDRKVLAHFHFQSRAPSSRSNS---YG 71
           CL +LFA   +  S++  T +E + E L +K    R     F F+  A +  ++S     
Sbjct: 6   CL-LLFAYSLLSFSLTAATIDETYDESLFIKSFSSRYSYVSFAFEIGASTDSTHSSVFSE 65

Query: 72  RHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPS---GVELWAV 131
               LFP +I++++ + ++ E+ +  T+GRW Y+ W      S  N   S   G E+WA 
Sbjct: 66  SSFSLFPLSIARVMDECQVSELHIRATRGRWDYENW----KESPDNGFYSGGLGFEVWAF 125

Query: 132 FDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSY----SAPKWGFHPASENMRY-GTL 191
               P+     W  LT+ LSGL CAS+N+++SS +Y    S P  G    S N +Y  +L
Sbjct: 126 MANDPSM--KYWLKLTNQLSGLLCASLNYIDSSNTYQPQLSYP--GSFSFSNNTQYFASL 185

Query: 192 PREAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAI 251
           P+E VCTENL+P  KLLPC+ K+G++ L+D    +   +HS  + +  +E  S A     
Sbjct: 186 PQEDVCTENLSPLFKLLPCKRKAGIASLLDSHLFFDTDWHSFSIDVYPSENQSLASVKMG 245

Query: 252 VLDQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKG 311
           ++ Q +  V +    +G   +   ++      + S+        C+++  S  +  +D  
Sbjct: 246 IIIQAVVDVERNGRRKGKTTFQPPSEYCHDEDMDSL-------HCLMSGYSTEHHTVD-- 305

Query: 312 LMAELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSV 371
              +L   + ++  L  +  S             +N D++                    
Sbjct: 306 ---DLFHKVPKERCLLSSTFSD---------VFVSNGDKI-------------------- 365

Query: 372 AEKYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIP 431
                ++  LD     +IP+  +     + V R +   GN  G+    L ST  +    P
Sbjct: 366 -----DTFSLDEAANIQIPIQSTSDNHTVTVDRSLSNDGNHWGS----LSSTIYNPSSSP 425

Query: 432 DTVITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGV 491
            T++          F+  PW+++VY HTL + ++    +  + +EK+   P  D+ +  +
Sbjct: 426 RTIV---------YFEKFPWFVRVYLHTLTITLNGTRINTKDFIEKLYYQPLRDRKAGTM 485

Query: 492 LEMLLKFPCGLKSAALTIEF--DKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAE 551
           +E+    P       L + F  +K  L +DEYPPDA++G ++P A+I+         F E
Sbjct: 486 MEIQFSIP---PHTNLIVHFNVEKTPLRLDEYPPDANRGYNLPPAIISV--------FDE 545

Query: 552 NNSSKSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLR 611
           NN+    +             T  LL+   TPDFSMPYNVI  T TV+AL FG + N+L 
Sbjct: 546 NNTKLCSL------------RTAALLMFIPTPDFSMPYNVIIFTSTVIALTFGGIFNLLT 545

Query: 612 RR-VGEEERFMKDKAKKKFQVSQLVSKLLAKLRG 630
           RR V ++ +F     ++   + +L  K+  K RG
Sbjct: 606 RRFVPQQSKFQN---RQPSMLQRLKEKIFHKKRG 545

BLAST of Carg25522 vs. TrEMBL
Match: tr|A0A1S3C6Z6|A0A1S3C6Z6_CUCME (GPI transamidase component PIG-T OS=Cucumis melo OX=3656 GN=LOC103497563 PE=4 SV=1)

HSP 1 Score: 1216.8 bits (3147), Expect = 0.0e+00
Identity = 604/669 (90.28%), Postives = 633/669 (94.62%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MASFLRLISLLCLPILFAV  IGSVSEG EEDFSEDLLLKPLPDRKVLAHFHFQS APSS
Sbjct: 1   MASFLRLISLLCLPILFAVSVIGSVSEGAEEDFSEDLLLKPLPDRKVLAHFHFQSTAPSS 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL
Sbjct: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDVPPNEVDA+WKNLTHSLSGLFCASINFLESSTSYSAPKW FHPASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDATWKNLTHSLSGLFCASINFLESSTSYSAPKWSFHPASENMRYGTLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKG+YHSQRLHLLS+EFDS+ VDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGYYHSQRLHLLSSEFDSDGVDSAIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           DQTLTVVLQP+SHRG LGYS+A QLQPSWSLSSIFGR+V G+C LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPHSHRGTLGYSSATQLQPSWSLSSIFGRRVIGKCSLARSSNVYIQLDRGLM 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
           AELQG+LGEQE  A+ R+SSEGSI N AFELSANPDRVHMEMS+  DKH+SVLYMF VA 
Sbjct: 301 AELQGILGEQEKFAIARASSEGSISNPAFELSANPDRVHMEMSNGYDKHISVLYMFMVAA 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           KYD+SEPLDLRFTWKIP+ WSIPQAPL+VTRF+LGSGNERG IALQLKSTKP DQL+PDT
Sbjct: 361 KYDDSEPLDLRFTWKIPIAWSIPQAPLYVTRFLLGSGNERGVIALQLKSTKPRDQLMPDT 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           V  DSCSLLV+VFQVVPWYIKVYYHTLQVFIDDQ H ITNVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VFADSCSLLVRVFQVVPWYIKVYYHTLQVFIDDQRHIITNVVEKMQVSPSKDKVSPGVME 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDFFTSTQF E NSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFFTSTQFVEKNSS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
           KSPIL KWQG++PILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILLKWQGKNPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600

Query: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
           EEERFMK KA +K +VS LVSKLLAKLRGR WEPSESQ+ SS SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKSKATRKLRVSHLVSKLLAKLRGRQWEPSESQSHSSVSNSKSKLLFKVILVAGL 660

Query: 661 AAAWQYYFG 670
           AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669

BLAST of Carg25522 vs. TrEMBL
Match: tr|A0A0A0K6R2|A0A0A0K6R2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G340200 PE=4 SV=1)

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 600/669 (89.69%), Postives = 629/669 (94.02%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MASFLRLISLLCLPILFAV   GSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS
Sbjct: 1   MASFLRLISLLCLPILFAVSVNGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYD WGGFD ISSSNAKPSGVEL
Sbjct: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDHWGGFDLISSSNAKPSGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDV PNEVDA+WKNLTHSLSGLFCASINFLESSTSYSAPKW FHP SENMRYGTLPR
Sbjct: 121 WAVFDVLPNEVDATWKNLTHSLSGLFCASINFLESSTSYSAPKWSFHPESENMRYGTLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKG+YHSQRLHLLS+EFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGYYHSQRLHLLSSEFDSNAVDSAIVL 240

Query: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
           DQTLTVVLQP++HRG LGYS A QLQPSWSLS+IFGR+V G+C LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPHNHRGTLGYSTATQLQPSWSLSTIFGRRVIGKCSLARSSNVYIQLDRGLM 300

Query: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
           AELQGMLGEQEM ++ R+  EGS  N AFELSANPDRVHMEMSSR DKH SVLYMF VAE
Sbjct: 301 AELQGMLGEQEMFSIARAGFEGSRSNPAFELSANPDRVHMEMSSRYDKHASVLYMFMVAE 360

Query: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
           KYD+SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+PDT
Sbjct: 361 KYDDSEPLDLRFTWKIPVAWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420

Query: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
           V  D+CSLLV+VFQVVPWYIKVYYHTL +FI+DQPH I NV+EKMQVSPS+DKVSPGV+E
Sbjct: 421 VFADNCSLLVRVFQVVPWYIKVYYHTLHIFINDQPHKINNVIEKMQVSPSKDKVSPGVME 480

Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
           MLLK PCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKLPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540

Query: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
           KSPIL KWQGQ+PILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILLKWQGQNPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600

Query: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
           EEERFMK KA +K +V  LVSKLLAKLRGR WEPSESQ+PSS SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKSKATRKLRVLHLVSKLLAKLRGRQWEPSESQSPSSVSNSKSKLLFKVILVAGL 660

Query: 661 AAAWQYYFG 670
           AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669

BLAST of Carg25522 vs. TrEMBL
Match: tr|A0A061DV55|A0A061DV55_THECC (GPI transamidase component Gpi16 subunit family protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_002897 PE=4 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 1.3e-252
Identity = 452/671 (67.36%), Postives = 537/671 (80.03%), Query Frame = 0

Query: 5   LRLISLLCLPILFAVPAIGSVSE--GTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSSRS 64
           L L+ L+   +L    A GSV E    +E+F+E+LLLKPLPDRKVLAHFHFQS AP S S
Sbjct: 9   LVLLLLVTHHLLQCWIAFGSVDEEGQKQEEFNEELLLKPLPDRKVLAHFHFQSSAPPSTS 68

Query: 65  NSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVELWA 124
           N  GRHHHLFPKAISQLV K+R+KEMELSFTQGRW Y+RWGGFD I+SSNAKP GVELWA
Sbjct: 69  N--GRHHHLFPKAISQLVQKFRVKEMELSFTQGRWNYERWGGFDPIASSNAKPPGVELWA 128

Query: 125 VFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPREA 184
           VFDVP ++VDASWKNLTH+LSGLFCASINFLES+++YSAP+W F P+S N+RYGTLPREA
Sbjct: 129 VFDVPQHQVDASWKNLTHALSGLFCASINFLESTSTYSAPEWSFPPSSGNLRYGTLPREA 188

Query: 185 VCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVLDQ 244
           VCTENLTPWLKLLPCRDK+GL+VL+DRPSIY+GFYHSQRLHL ST   S   DS I+L+Q
Sbjct: 189 VCTENLTPWLKLLPCRDKAGLAVLLDRPSIYRGFYHSQRLHLTSTGSGSKGTDSGIILNQ 248

Query: 245 TLTVVLQPYSHRG---ALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGL 304
            LTVVLQP    G   ++ +++   +QPSWSLSSIFG++V GRCVLA+SS VY+QLD+GL
Sbjct: 249 ALTVVLQPIQPNGLRASMDHASEKHIQPSWSLSSIFGKQVRGRCVLAKSSKVYLQLDRGL 308

Query: 305 MAELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVA 364
           +AEL+ +  E E       +SE      +FELSANPDR+ +E +S   K  S+LY F V 
Sbjct: 309 VAELKRIHKENEKSVANGLTSENFWSYPSFELSANPDRIVIEENSLHSKSSSILYNFQV- 368

Query: 365 EKYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPD 424
           EKY ESEPLDL  TWKIPVVW    APLH +RF++GSGNERGAIA+ LKST+  +  +  
Sbjct: 369 EKYSESEPLDLGLTWKIPVVWLCQSAPLHSSRFLMGSGNERGAIAISLKSTQLREGFMGA 428

Query: 425 TVITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVL 484
               + C L V VFQVVPWY+KVY+H+LQVFID QP ++++V+EK+ VSPS+DKVSPG++
Sbjct: 429 FADNERCELRVDVFQVVPWYVKVYFHSLQVFIDQQPKAVSDVIEKIHVSPSKDKVSPGMM 488

Query: 485 EMLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNS 544
           EM+LK PC +KSAALTIEFDKGFLHIDEYPPDA+QG DIPSA+I+FP+F  S  F E+ S
Sbjct: 489 EMVLKLPCRVKSAALTIEFDKGFLHIDEYPPDANQGFDIPSAIISFPNFHASMFFLEDGS 548

Query: 545 -SKSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRR 604
            +KSP+LSK+Q +SP++SYTEVLLVP TTPDFSMPYNVITITCTV ALYFGSLLNVLRRR
Sbjct: 549 VNKSPLLSKFQEKSPVMSYTEVLLVPLTTPDFSMPYNVITITCTVFALYFGSLLNVLRRR 608

Query: 605 VGEEERFMKDKAKKKF-QVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILV 664
           + EEERF+KDKA KK  Q+  L+SKL AKLRGR WEP  S  PS+S    SKL+FKVILV
Sbjct: 609 IAEEERFLKDKATKKTGQLPLLLSKLSAKLRGRPWEPPHS-PPSTSFFISSKLVFKVILV 668

Query: 665 AGLAAAWQYYF 669
           AGLA  WQYYF
Sbjct: 669 AGLAVGWQYYF 675

BLAST of Carg25522 vs. TrEMBL
Match: tr|A0A2I4GV09|A0A2I4GV09_9ROSI (GPI transamidase component PIG-T OS=Juglans regia OX=51240 GN=LOC109011120 PE=4 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 1.3e-252
Identity = 447/667 (67.02%), Postives = 538/667 (80.66%), Query Frame = 0

Query: 5   LRLISLLCLPILFAVPAIGS-VSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSSRSN 64
           L L  +L L I+F+  A GS   E  EE+FSE+LLL+PLPDRKVLAHFHF+SRAP S S+
Sbjct: 9   LSLFLVLSLQIIFSAIAFGSDFQESDEEEFSEELLLRPLPDRKVLAHFHFESRAPPSLSD 68

Query: 65  SYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVELWAV 124
           S+GRHHHLFPKAI+QLV K+R+KEMELSFTQGRW YDRWGGFD ISSSNAKP GVELWA 
Sbjct: 69  SHGRHHHLFPKAIAQLVQKFRVKEMELSFTQGRWNYDRWGGFDPISSSNAKPPGVELWAA 128

Query: 125 FDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPREAV 184
           FDVP   VDASWKNLTH+LSGLFCASINFLESST+YSAP+W FHP+S ++RYGTLPREAV
Sbjct: 129 FDVPQYLVDASWKNLTHTLSGLFCASINFLESSTTYSAPEWSFHPSSGSLRYGTLPREAV 188

Query: 185 CTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVLDQT 244
           CTENLTPWLKLLPCRDK+GLSVLMDRPSIY+GFYHSQ+L L S EF+   ++S IVL QT
Sbjct: 189 CTENLTPWLKLLPCRDKAGLSVLMDRPSIYRGFYHSQQLRLTSPEFEGR-LESGIVLKQT 248

Query: 245 LTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLMAEL 304
           LTVVLQP S   +  YS+  +LQPSWSLSSIFGRKV  RCVLA+SSN+Y+QL+ GL+ EL
Sbjct: 249 LTVVLQPDSQGASNTYSSETKLQPSWSLSSIFGRKVTSRCVLAKSSNIYLQLEGGLVTEL 308

Query: 305 QGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAEKYD 364
           + +  E  +  +    SEG   N  F+LS NPD V  E++S      SVLY +SVA KY 
Sbjct: 309 ENVRKENVISWIDNVGSEGWRTNPFFDLSVNPDSVLREVNSLHGSSSSVLYEYSVA-KYS 368

Query: 365 ESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDTVIT 424
           +S P DL   WK PVVWS PQAPLH +RF++GSGNERGAIA+ L ST  ++  +   ++ 
Sbjct: 369 DSRPFDLGLKWKSPVVWSCPQAPLHASRFLMGSGNERGAIAISLISTGLNEHFLGANIVE 428

Query: 425 DSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLEMLL 484
            SC L V +FQVVPWY+KVYYHTL+VF++ QP ++T+VVEKM+VSPSEDKVSPGV+E++L
Sbjct: 429 GSCKLEVDIFQVVPWYVKVYYHTLRVFVNGQPQAVTDVVEKMRVSPSEDKVSPGVMELVL 488

Query: 485 KFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSSK-S 544
           KFPC +KSAALT+EFDKGFLHIDEYPPDA+QG DIPSA+I+FP+F   T + E++S K S
Sbjct: 489 KFPCDVKSAALTLEFDKGFLHIDEYPPDANQGFDIPSAIISFPNFHARTHYFEDSSLKRS 548

Query: 545 PILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVGEE 604
           P+LSK+Q +S + SY+EVLLVP TTPDFSMPYNVITITCT  ALYFGSLLNVLRRRVGEE
Sbjct: 549 PMLSKFQEKSRVRSYSEVLLVPLTTPDFSMPYNVITITCTAFALYFGSLLNVLRRRVGEE 608

Query: 605 ERFMKDKAKKKF-QVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKS-KLLFKVILVAGL 664
           ERF+K++A KK  +++QL+S L AK+RG+ WE    Q+PS+SS+  S KL+ KV+LV G+
Sbjct: 609 ERFLKNRAAKKAGRLAQLLSMLSAKIRGKKWE--HPQSPSASSSFISLKLIVKVVLVTGI 668

Query: 665 AAAWQYY 668
           A AWQYY
Sbjct: 669 AIAWQYY 671

BLAST of Carg25522 vs. TrEMBL
Match: tr|F6HQ50|F6HQ50_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_07s0104g00690 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.1e-250
Identity = 446/672 (66.37%), Postives = 534/672 (79.46%), Query Frame = 0

Query: 1   MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
           MA+ LR    L   +LF+  A             E+LLLKPL DRKVLAHFHF+S+AP +
Sbjct: 1   MAAVLR---FLIAVLLFSSIASAXXXXXXXXXXXEELLLKPLADRKVLAHFHFESKAPPT 60

Query: 61  RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
           R  +YG HH LFPKA+ QLV K+RI+EMELSFTQGRW Y+RWGGFD ISSSNAKP GVEL
Sbjct: 61  R--TYGHHHRLFPKALYQLVQKFRIREMELSFTQGRWNYERWGGFDPISSSNAKPPGVEL 120

Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
           WAVFDVP + VDASWKNLTH+LSGLFCASINFLESST+YSAP WGF P S ++RYG+LPR
Sbjct: 121 WAVFDVPHDLVDASWKNLTHTLSGLFCASINFLESSTAYSAPDWGFQPFSGSLRYGSLPR 180

Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
           EAVCTENLTPWLKLLPCRDK+GL+ LMDRPSIY+GFYHSQRL L STEF S  ++S I L
Sbjct: 181 EAVCTENLTPWLKLLPCRDKAGLAALMDRPSIYRGFYHSQRLRLSSTEFGSTELESGIAL 240

Query: 241 DQTLTVVLQP-YSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGL 300
           DQTLTVVLQP  S R ++ YS+ + LQPSWSLSSIFGRKV GRCVLA+SSNVY+Q+++GL
Sbjct: 241 DQTLTVVLQPTTSQRASMTYSSDSVLQPSWSLSSIFGRKVSGRCVLAKSSNVYVQVERGL 300

Query: 301 MAELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVA 360
           ++EL+ +  E E       + E +  N  FELS  P+RV  E++   D+   VLY + + 
Sbjct: 301 VSELKNLHAEDEGSGAGNVTFEKTWNNPGFELSIAPNRVIKEVNILHDETSIVLYEYLI- 360

Query: 361 EKYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPD 420
           + Y  SEP DL FTWK PVVWS PQAPLH +RF++GSGNERGAIA+ LK+T+PSD L   
Sbjct: 361 DNYSNSEPFDLGFTWKFPVVWSSPQAPLHASRFLMGSGNERGAIAISLKATEPSDGLWVA 420

Query: 421 TVITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVL 480
             I +SC L   VFQVVPWY++VY+HTLQVF+D +P S+T+ +E+M++SPSEDKVSPGV+
Sbjct: 421 DTIENSCMLRFNVFQVVPWYVRVYFHTLQVFVDGKPQSVTDFIERMRISPSEDKVSPGVM 480

Query: 481 EMLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNS 540
           EM LK PC +KS  LT+EFDKGFLHIDEYPPDA+QG DIPSAVI+FPDF  S  F +++S
Sbjct: 481 EMALKLPCCVKSVTLTLEFDKGFLHIDEYPPDANQGFDIPSAVISFPDFHASMHFLKDDS 540

Query: 541 -SKSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRR 600
            +KSP+LSK+Q +SP+L YTEVLLVP TTPDFSMPYNVITITCTV ALYFGSLLNVLRRR
Sbjct: 541 LNKSPLLSKFQEKSPVLCYTEVLLVPLTTPDFSMPYNVITITCTVFALYFGSLLNVLRRR 600

Query: 601 VGEEERFMKDK-AKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILV 660
           VGEEERF+K K +KK  ++ QL+SKL AKLRG+ WEP+++Q+ SSS  S SKL+FKVILV
Sbjct: 601 VGEEERFLKRKDSKKTGRLGQLLSKLFAKLRGKSWEPTQTQSASSSLIS-SKLIFKVILV 660

Query: 661 AGLAAAWQYYFG 670
           AG+AA WQYY G
Sbjct: 661 AGIAAGWQYYSG 665