Carg25515 (gene) Silver-seed gourd

BLAST of Carg25515 vs. NCBI nr
Match: XP_022961087.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucurbita moschata])

HSP 1 Score: 3526.5 bits (9143), Expect = 0.0e+00
Identity = 1757/1768 (99.38%), Postives = 1761/1768 (99.60%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
            LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240
            TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM
Sbjct: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240

Query: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300
            STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360
            EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420
            ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420

Query: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540

Query: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660

Query: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNA 720
            STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRL KSNA
Sbjct: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLVKSNA 720

Query: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780
            TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG
Sbjct: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780

Query: 781  KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNI 840
            KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNI
Sbjct: 781  KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNI 840

Query: 841  LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH 900
            LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH
Sbjct: 841  LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH 900

Query: 901  CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL 960
            CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL
Sbjct: 901  CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL 960

Query: 961  SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSII 1020
            SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI+VKSVHIPPSII
Sbjct: 961  SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVKSVHIPPSII 1020

Query: 1021 DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE 1080
            DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE
Sbjct: 1021 DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE 1080

Query: 1081 QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK 1140
            QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK
Sbjct: 1081 QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK 1140

Query: 1141 HSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGN 1200
            HSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGN
Sbjct: 1141 HSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGN 1200

Query: 1201 SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL 1260
            SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL
Sbjct: 1201 SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL 1260

Query: 1261 DAVWKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDS 1320
            DAVWKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDS
Sbjct: 1261 DAVWKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDS 1320

Query: 1321 VETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDT 1380
            VETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDT
Sbjct: 1321 VETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDT 1380

Query: 1381 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG 1440
            VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG
Sbjct: 1381 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG 1440

Query: 1441 SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK 1500
            SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK
Sbjct: 1441 SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK 1500

Query: 1501 RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ 1560
            RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ
Sbjct: 1501 RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ 1560

Query: 1561 FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL 1620
            FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL
Sbjct: 1561 FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL 1620

Query: 1621 YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI 1680
            YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI
Sbjct: 1621 YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI 1680

Query: 1681 DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ 1740
            DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ
Sbjct: 1681 DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ 1740

Query: 1741 YKKRFRKAMTTYFLMINGPPPRSALVSP 1769
            YKKRFRKAMTTYFLM+    P S+ +SP
Sbjct: 1741 YKKRFRKAMTTYFLMVPDQWP-SSTISP 1767

BLAST of Carg25515 vs. NCBI nr
Match: XP_023515845.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3498.4 bits (9070), Expect = 0.0e+00
Identity = 1742/1768 (98.53%), Postives = 1752/1768 (99.10%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFCSKCTANFIPSPSTDP+NAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPSNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
            LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240
            TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM
Sbjct: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240

Query: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300
            STKGSPVNFDTQSPRGDEES EDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGDEESGEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360
            EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420
            ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420

Query: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540

Query: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPH+PSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHTPSNIDRSI 660

Query: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNA 720
            S IPGFTCPSPVKSPEPE SMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRL KSNA
Sbjct: 661  SAIPGFTCPSPVKSPEPEPSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLVKSNA 720

Query: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780
            TSTSLSFLKQNVSTADRNY+LSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG
Sbjct: 721  TSTSLSFLKQNVSTADRNYILSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780

Query: 781  KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNI 840
            KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQR ESL+ANLVTTKSSREDFPLSPSDQQNI
Sbjct: 781  KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRTESLVANLVTTKSSREDFPLSPSDQQNI 840

Query: 841  LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH 900
            LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH
Sbjct: 841  LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH 900

Query: 901  CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL 960
            CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL
Sbjct: 901  CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL 960

Query: 961  SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSII 1020
            SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI+VKSVHIPPSII
Sbjct: 961  SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVKSVHIPPSII 1020

Query: 1021 DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE 1080
            DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE
Sbjct: 1021 DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE 1080

Query: 1081 QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK 1140
            QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK
Sbjct: 1081 QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK 1140

Query: 1141 HSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGN 1200
            HSPDSSSNDIPTLEEKELDSMEELVELTLAFG DDLFGGFDNDSLPSESKHDVDLDRGGN
Sbjct: 1141 HSPDSSSNDIPTLEEKELDSMEELVELTLAFGQDDLFGGFDNDSLPSESKHDVDLDRGGN 1200

Query: 1201 SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL 1260
            SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL
Sbjct: 1201 SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL 1260

Query: 1261 DAVWKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDS 1320
            DAVWKGALHPNS+ FDDRYPQT+SSILEPLAGPDMEKCTANGVETARLLYSALVLT VDS
Sbjct: 1261 DAVWKGALHPNSIQFDDRYPQTNSSILEPLAGPDMEKCTANGVETARLLYSALVLTRVDS 1320

Query: 1321 VETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDT 1380
            VETCTSL SMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLR FMHVDSSDT
Sbjct: 1321 VETCTSLMSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRFFMHVDSSDT 1380

Query: 1381 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG 1440
            VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG
Sbjct: 1381 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG 1440

Query: 1441 SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK 1500
            SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK
Sbjct: 1441 SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK 1500

Query: 1501 RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ 1560
            RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ
Sbjct: 1501 RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ 1560

Query: 1561 FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL 1620
            FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL
Sbjct: 1561 FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL 1620

Query: 1621 YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI 1680
            YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI
Sbjct: 1621 YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI 1680

Query: 1681 DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ 1740
            DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ
Sbjct: 1681 DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ 1740

Query: 1741 YKKRFRKAMTTYFLMINGPPPRSALVSP 1769
            YKKRFRKAMTTYFLM+    P S+ +SP
Sbjct: 1741 YKKRFRKAMTTYFLMVPDQWP-SSTISP 1767

BLAST of Carg25515 vs. NCBI nr
Match: XP_023515846.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3491.8 bits (9053), Expect = 0.0e+00
Identity = 1735/1768 (98.13%), Postives = 1745/1768 (98.70%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFCSKCTANFIPSPSTDP+NAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPSNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
            LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240
            TSLMTSSSSYYGYC SDDEDG XXXXXXX     RHIPQSDHYYCSANTEEGLNLERENM
Sbjct: 181  TSLMTSSSSYYGYC-SDDEDGDXXXXXXXSDSESRHIPQSDHYYCSANTEEGLNLERENM 240

Query: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300
            STKGSPVNFDTQSPRGDEES EDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGDEESGEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360
            EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420
            ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420

Query: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540

Query: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPH+PSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHTPSNIDRSI 660

Query: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNA 720
            S IPGFTCPSPVKSPEPE SMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRL KSNA
Sbjct: 661  SAIPGFTCPSPVKSPEPEPSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLVKSNA 720

Query: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780
            TSTSLSFLKQNVSTADRNY+LSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG
Sbjct: 721  TSTSLSFLKQNVSTADRNYILSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780

Query: 781  KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNI 840
            KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQR ESL+ANLVTTKSSREDFPLSPSDQQNI
Sbjct: 781  KGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRTESLVANLVTTKSSREDFPLSPSDQQNI 840

Query: 841  LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH 900
            LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH
Sbjct: 841  LVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVH 900

Query: 901  CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL 960
            CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL
Sbjct: 901  CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGL 960

Query: 961  SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSII 1020
            SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI+VKSVHIPPSII
Sbjct: 961  SFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVKSVHIPPSII 1020

Query: 1021 DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE 1080
            DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE
Sbjct: 1021 DFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELE 1080

Query: 1081 QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK 1140
            QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK
Sbjct: 1081 QIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKK 1140

Query: 1141 HSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGN 1200
            HSPDSSSNDIPTLEEKELDSMEELVELTLAFG DDLFGGFDNDSLPSESKHDVDLDRGGN
Sbjct: 1141 HSPDSSSNDIPTLEEKELDSMEELVELTLAFGQDDLFGGFDNDSLPSESKHDVDLDRGGN 1200

Query: 1201 SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL 1260
            SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL
Sbjct: 1201 SGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTL 1260

Query: 1261 DAVWKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDS 1320
            DAVWKGALHPNS+ FDDRYPQT+SSILEPLAGPDMEKCTANGVETARLLYSALVLT VDS
Sbjct: 1261 DAVWKGALHPNSIQFDDRYPQTNSSILEPLAGPDMEKCTANGVETARLLYSALVLTRVDS 1320

Query: 1321 VETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDT 1380
            VETCTSL SMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLR FMHVDSSDT
Sbjct: 1321 VETCTSLMSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRFFMHVDSSDT 1380

Query: 1381 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG 1440
            VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG
Sbjct: 1381 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG 1440

Query: 1441 SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK 1500
            SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK
Sbjct: 1441 SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK 1500

Query: 1501 RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ 1560
            RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ
Sbjct: 1501 RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ 1560

Query: 1561 FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL 1620
            FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL
Sbjct: 1561 FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL 1620

Query: 1621 YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI 1680
            YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI
Sbjct: 1621 YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI 1680

Query: 1681 DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ 1740
            DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ
Sbjct: 1681 DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ 1740

Query: 1741 YKKRFRKAMTTYFLMINGPPPRSALVSP 1769
            YKKRFRKAMTTYFLM+    P S+ +SP
Sbjct: 1741 YKKRFRKAMTTYFLMVPDQWP-SSTISP 1766

BLAST of Carg25515 vs. NCBI nr
Match: XP_022987999.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucurbita maxima])

HSP 1 Score: 3441.7 bits (8923), Expect = 0.0e+00
Identity = 1722/1772 (97.18%), Postives = 1737/1772 (98.02%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSW+PWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWIPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDG+KATCSAIS
Sbjct: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGNKATCSAIS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
            LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCS SIADEQDSLRNGKTIKRS
Sbjct: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSVSIADEQDSLRNGKTIKRS 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240
            TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHI QS HYYCSANTEEGLNLERENM
Sbjct: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHISQSGHYYCSANTEEGLNLERENM 240

Query: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300
            STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360
            EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR
Sbjct: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420
            ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSG MDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKRTESIIVKGIICKKNVAHRRM KEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRTESIIVKGIICKKNVAHRRMTKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540

Query: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660

Query: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNA 720
            S IPGFTCPSPVKSPEPE SMETGQSGNDAVPTRDSS+ISHNVDNLNSLEPSLRL KSNA
Sbjct: 661  SAIPGFTCPSPVKSPEPEPSMETGQSGNDAVPTRDSSIISHNVDNLNSLEPSLRLVKSNA 720

Query: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKE----DDDTV 780
            TST+LSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMG+MTIKEEAKE    DDDTV
Sbjct: 721  TSTNLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGMMTIKEEAKEDAXXDDDTV 780

Query: 781  SSSGKGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSD 840
            SSSGKGVPL+ GNSIHIRS+SLDANTQ SNDSTCQR ESLIANLVTTKSSREDFPLSPSD
Sbjct: 781  SSSGKGVPLTRGNSIHIRSNSLDANTQGSNDSTCQRTESLIANLVTTKSSREDFPLSPSD 840

Query: 841  QQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSE 900
            QQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSE
Sbjct: 841  QQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSE 900

Query: 901  AHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDA 960
            AHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDA
Sbjct: 901  AHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDA 960

Query: 961  AWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIP 1020
            AWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI+VKSVHIP
Sbjct: 961  AWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVKSVHIP 1020

Query: 1021 PSIIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYI 1080
            PSII+FHHNNLEWINKEANEVHNRAEVLFA VCKTLPLILKHESGTEQEDFGVNELNNYI
Sbjct: 1021 PSIIEFHHNNLEWINKEANEVHNRAEVLFAEVCKTLPLILKHESGTEQEDFGVNELNNYI 1080

Query: 1081 QELEQIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVT 1140
            QELEQIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVT
Sbjct: 1081 QELEQIINVEKEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVT 1140

Query: 1141 LGKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLD 1200
            LGKKHSPDSSSNDIPTLEEK LDSMEELVELTLAFG DDLFGGFDNDSLPSESKHDVDLD
Sbjct: 1141 LGKKHSPDSSSNDIPTLEEKGLDSMEELVELTLAFGQDDLFGGFDNDSLPSESKHDVDLD 1200

Query: 1201 RGGNSGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDL 1260
            RGGNSGQMNRF ETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEY SMTDL
Sbjct: 1201 RGGNSGQMNRFYETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYSSMTDL 1260

Query: 1261 SGTLDAVWKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLT 1320
            SGTL AVWKGA+HPNS+LFDDRYPQT+SSILEPLAGPDMEKCTANGVE   LLYSALVLT
Sbjct: 1261 SGTLVAVWKGAIHPNSILFDDRYPQTNSSILEPLAGPDMEKCTANGVEITHLLYSALVLT 1320

Query: 1321 GVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVD 1380
             VDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELE+QGHLRLFMHV 
Sbjct: 1321 RVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELERQGHLRLFMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTC 1440
            SSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKM SIKTC
Sbjct: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMGSIKTC 1440

Query: 1441 DEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITC 1500
            DEEGSDVYRTL SNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITC
Sbjct: 1441 DEEGSDVYRTLGSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITC 1500

Query: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
            YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE
Sbjct: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHN 1620
            SFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQV TKHLKGGKEFKIDVLVMENLLFGHN
Sbjct: 1561 SFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVFTKHLKGGKEFKIDVLVMENLLFGHN 1620

Query: 1621 VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSF 1680
            VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSF 1680

Query: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740
            LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTTYFLMINGPPPRSALVSP 1769
            SPHQYKKRFRKAMTTYFLM+    P S+ VSP
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWP-SSTVSP 1771

BLAST of Carg25515 vs. NCBI nr
Match: XP_016899810.1 (PREDICTED: LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucumis melo])

HSP 1 Score: 2942.5 bits (7627), Expect = 0.0e+00
Identity = 1478/1772 (83.41%), Postives = 1568/1772 (88.49%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPWRSDP NVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFCSKCTANFIP+ STDPTNAREDSE IRVCNYCFKQWGKEPS+L DGDKAT SA+S
Sbjct: 61   CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
            +SSSSTS+GSTKSGYTC SAIS+I STPCS  RQYYDP+CS +IADE D+LRNGKTI R+
Sbjct: 121  VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240
                                 XXXXX        RHI QSD YYC+ N + GLNLEREN 
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240

Query: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300
            STKGSPVNFDTQ PRG+EESSEDSDENDKGSECDFPTY MQNKD+E MDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360
            E                  CAPGEWGHL SS  SGECPMK KSS DY++TAKNVVEGHFR
Sbjct: 301  EXXXXXXXXXXXXXXXXXVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420
            ALVSQLLEAENLP+GD PNEDGWLDI+TYLSWEAAAVLKPDTSKSG MDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKR+ESIIVKGIICKKNVAHRRM + ISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK ITLVLN+KRSLLERIACCTGANI+
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVA LKLGYCD FRV+TFVEEH SGDGQ GKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPL SPI V LPH+PSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNA 720
            S IPGFTCP PVKSPEPE  ME GQSG DAV +RD S+ISHNV+NLNSLEP+L L +SNA
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLLESNA 720

Query: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780
            TSTS SFLKQ+ ST     MLSSK  SE  R+ DS+EYSMG+MTIKEEA+ED D VSSSG
Sbjct: 721  TSTSFSFLKQDFST-----MLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSG 780

Query: 781  KGVPLS-HGNS-IHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQ 840
            K    S H NS I +  +SLD+NTQS NDST Q PE+   +LVTTKSSREDFPLSPSDQQ
Sbjct: 781  KREASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQ 840

Query: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900
            NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH
Sbjct: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900

Query: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960
            VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW
Sbjct: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960

Query: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPS 1020
            GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI+V+SVHIPPS
Sbjct: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020

Query: 1021 IIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQE 1080
            +I+FH NNLEWINKEANEVHNRA+ LF  VCK LPLI K+ESG EQEDFG +ELNNYIQE
Sbjct: 1021 MIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQE 1080

Query: 1081 LEQIINVEKEDFEVHFNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTL 1140
            LEQIIN+EKE+FEVHFNK  SG TTV QPTVDIFEINKLHMH+LFLSYIWVQRL+Y  TL
Sbjct: 1081 LEQIINMEKENFEVHFNKVLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATL 1140

Query: 1141 GKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDR 1200
             KKHSPD SSNDI TLEEKEL+S+EELVELTLA G D+ FGG DNDSLP ESK +  L R
Sbjct: 1141 RKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPESKREAGLGR 1200

Query: 1201 GGNSGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLS 1260
             GNSG ++R NET REKNIDLGGQEDD CL  VNSEP+DVG  +RR NS GEYP MTDLS
Sbjct: 1201 EGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLS 1260

Query: 1261 GTLDAVWKGALHPNSMLFDDRYPQTSSSILEPLAG-PDMEKCTANGVETARLLYSALVLT 1320
            GTLDAVWKG +HPNS++F+D + +T+S+ILEPLAG  DMEKCTANG+ETA LL+SALVLT
Sbjct: 1261 GTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAHLLHSALVLT 1320

Query: 1321 GVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVD 1380
              DSVET T+ TSMP SAY++KISLLNIPK DISDY    I+S+RELEKQ HLRL MHV 
Sbjct: 1321 SDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTC 1440
            SSDTVVPVYDEEPTSIIAYSLLMPEY AQMSEP + MEA DTARSLP IGSVKM+SIKTC
Sbjct: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSVKMNSIKTC 1440

Query: 1441 DEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITC 1500
            DEEGSD Y+TLRSNEETILSM RTESLQFEDL+  TKDLHTRVCF+NETPLGPVKYTITC
Sbjct: 1441 DEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLGPVKYTITC 1500

Query: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
            YFAKRFEALRKKCCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIELE
Sbjct: 1501 YFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHN 1620
            SFIQF+ AYF+YLS++I TGCPTCLAKVVGMYQVS+KHLKGGKE K+DVLVMENLLF HN
Sbjct: 1561 SFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRHN 1620

Query: 1621 VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSF 1680
            VTRLYDLKGSSRSRYN DTSG NKVLLDQNLIESMPTSPIFLG KAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680

Query: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740
            LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTTYFLMINGPPPRSALVSP 1769
            SPHQYKKRFRKAMTTYFLM+    P S+ VSP
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWP-SSTVSP 1766

BLAST of Carg25515 vs. TAIR10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein)

HSP 1 Score: 1597.8 bits (4136), Expect = 0.0e+00
Identity = 919/1806 (50.89%), Postives = 1164/1806 (64.45%), Query Frame = 0

Query: 9    SDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCSK 68
            S++VGL+KSW+PWRS+P  VSRDFWMPD+SCRVCY+CD QFT INRRHHCR CGRVFC K
Sbjct: 12   SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69   CTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDK--ATCSAISLSSSST 128
            CTAN IP   +D    RED E IRVCNYCF+QW +      DG    +  + +S S S T
Sbjct: 72   CTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQG-----DGGPHVSNITELSTSPSET 131

Query: 129  SIGSTKSGYTCQS---AISNIPSTPCSNDRQYY--DPFCSGSIADEQDSLRNGKTIKRST 188
            S+ S+K+  T  S   A+ ++P     N R ++  D    G  + E    + GK   R +
Sbjct: 132  SLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRS 191

Query: 189  SLMTS-----SSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLE 248
            S + +     S       RSDDE                H P+++ YY       G+ ++
Sbjct: 192  SFIATDVEDPSRFALNSIRSDDE--YDEYGAYQTDIETSHSPRANEYY-GPMEYNGMGID 251

Query: 249  -----------RENMSTKGSPVNFDTQSP--RGDEESSEDSDENDKGSECDFPTYGMQNK 308
                        +  S  GSP+         R   E  +   E+D   EC+  +    + 
Sbjct: 252  DVPCKHLGGETADQKSLSGSPLIHQCLESLIREGSEQFQKKSEHDGRDECEASSPADISD 311

Query: 309  DN--EAMDFQNNGILWLPPEPEDEEDEK--ALLFNGDDDACAPGEWGHL--PSSQGSGEC 368
            D   E +DF+NNG+LW+PPEPE+EEDE+  AL    D++  A GEWG+L   +S GSGE 
Sbjct: 312  DQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSGEY 371

Query: 369  PMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAV 428
              + +++ ++++  KNVV+GHFRAL++QLL+ EN+ V DE  ++ WL+I+T LSWEAA +
Sbjct: 372  RGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANL 431

Query: 429  LKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLG 488
            LKPD SKSG MDPGGYVKVKC+A G R +S++VKG++CKKNV +RRM  +I K R  +LG
Sbjct: 432  LKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILG 491

Query: 489  GALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKI 548
            G LEYQRV+N LSSFDTLLQQE DHLKMAVAKI A  PN+LLVEKSVSR AQEYLL+K I
Sbjct: 492  GGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDI 551

Query: 549  TLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKS 608
            +LVLN+KR LL+RIA CTGA I+P+VDH++  KLGYC+ FRV  + EEHGS  GQ GKK 
Sbjct: 552  SLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGS-TGQVGKKV 611

Query: 609  TKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVP 668
             KTLM+FE CPKPL  TILLRGA+ DELKKVKHVVQY  FAAYHLALET FLADEGAS P
Sbjct: 612  VKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS-P 671

Query: 669  ELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSS 728
            ELPL SPI V LP   ++I+RSIST+PGFT  +  KSP   S  E  ++  ++VP  +  
Sbjct: 672  ELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRA--NSVPVSELL 731

Query: 729  MISHNVDNLNSLEPSLRLE--KSNATSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDS 788
              + N+     + P       ++   + S  F + N+S    + ++ S+ +  + R+   
Sbjct: 732  STTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSGRSVPV 791

Query: 789  EEYSMGIMTIKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPES 848
            +  +     I    +  ++++  SG+G       S  I +S +  N  +           
Sbjct: 792  DTPADKSNPIVVADETTNNSLHLSGQGFV---RKSSQIGTSIMVENQDNGXXXXXXXXXX 851

Query: 849  LIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGR 908
                           P SPSD Q+ILVSLS+R VWKGT+C+R+HL RIKYYGSFDKPLGR
Sbjct: 852  XXXXXXXXXXXXXXXP-SPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGR 911

Query: 909  FLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRC 968
            FLRD+LFDQ+YRC SC MPSEAHVHCYTH QGSLTIS KKL D+ LPGE EGKIWMWHRC
Sbjct: 912  FLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRC 971

Query: 969  LKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYG 1028
            L+CPR NGFPPAT RV+MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYG
Sbjct: 972  LRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYG 1031

Query: 1029 FGRTVACFHYAAIDVKSVHIPPSIIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLI 1088
            FG  VACF YA IDV SV++PPSI+ F++ N +WI +E +EV  RAE+LF+ V   +  I
Sbjct: 1032 FGNMVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQI 1091

Query: 1089 LKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHFNK-ASGETTVGQPTVDIFEIN 1148
               E G  +           I ELE+++  EK +FE +  K    E   GQP VDI E+ 
Sbjct: 1092 A--EKGFRRR----------IGELEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELY 1151

Query: 1149 KLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHD 1208
            ++H  +LF SY+W  RL  A TL K  + D +  +    E+  L   + L E+    G +
Sbjct: 1152 RIHRQLLFQSYMWDHRLINASTLHKLENSDDTKRE--ENEKPPLAKSQTLPEMNA--GTN 1211

Query: 1209 DLFGGFDNDSLPSESKHDVDLDRGGNSGQMNRFNETEREKNIDL------GGQEDDLCLY 1268
             L  G       SE   + D D  G++G +N   + E + N DL      GG+       
Sbjct: 1212 SLLTG-------SEVNLNPDGDSTGDTGSLNNV-QKEADTNSDLYQEKDDGGEVSPSKTL 1271

Query: 1269 QVNSEPLDVGTALRRTNSEGEYPSMTDLSGTLDAVWKG--------------ALHPNSML 1328
               S PL+    +RRT S+G+   M +LS TLDA W G              +L P++M 
Sbjct: 1272 PDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTMS 1331

Query: 1329 FDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDSVETCTSLTSMPSSA 1388
                +P  S  ++ P+  P+ +    N  + A  +  AL     ++ E   S  S+P   
Sbjct: 1332 NSSTFPPISEGLM-PIDLPEQQ----NEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLN 1391

Query: 1389 YSSKIS---LLNIPKPD-ISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPVYDEEPT 1448
            +   I+   LL+  K D   ++ P  I+SFRE E QG  RL + V  +D VVPVYD+EPT
Sbjct: 1392 FYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPT 1451

Query: 1449 SIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVYRTLRSN 1508
            S+IAY+L+ PEY  Q S     + +  +  ++P          +  D+   D  R+  S 
Sbjct: 1452 SMIAYALMSPEYQRQTSAEGESLVSYPSELNIP----------RPVDDTIFDPSRSNGSV 1511

Query: 1509 EETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEALRKKCC 1568
            +E+ILS++ + S    D L +TK LH RV +  +  LG VKYT+TCY+AKRFEALR  C 
Sbjct: 1512 DESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICL 1571

Query: 1569 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPAYFLYLS 1628
            PSEL+Y+RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+FAPAYF YLS
Sbjct: 1572 PSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLS 1631

Query: 1629 ESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRLYDLKGSSRSR 1688
            ESI T  PTCLAK++G+YQV+TK LK GKE K+DVL+MENLLFG  V RLYDLKGSSR+R
Sbjct: 1632 ESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRAR 1691

Query: 1689 YNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDVMDYSLLVG 1748
            YN D+SG NKVLLDQNLIE+MPTSPIF+G KAKRLLERAVWNDT+FLA  DVMDYSLLVG
Sbjct: 1692 YNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVG 1751

Query: 1749 LDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMT 1757
            +DEEK ELV+GIIDF+RQYTWDKHLE+WVK +GILGGPKN APTVISP QYK+RFRKAMT
Sbjct: 1752 VDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYKRRFRKAMT 1761

BLAST of Carg25515 vs. TAIR10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases)

HSP 1 Score: 1529.2 bits (3958), Expect = 0.0e+00
Identity = 875/1802 (48.56%), Postives = 1131/1802 (62.76%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P +S+  N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFC+KC AN IPSPS +  ++ E+ E IRVCNYC+KQW +      +G        S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
             S S+ S+ ST S             T                                 
Sbjct: 121  SSPSARSVASTTS--------XXXXCTIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXR------------------------- 240
                  SS +YG+     ++          XXXX                          
Sbjct: 181  XXXXRRSSDHYGHVLDSSDNQVEFFVNSSGXXXXXXXXXXXXXXXXXXXXXXGNDYYGAI 240

Query: 241  HIPQSDHYYCSANTEE-GLNLERENMSTKGSPVNFDTQSPRGDEESSEDSDENDKGSECD 300
            ++ + DH Y S    + G+ +E  N+S  G P + D  S   +        EN      +
Sbjct: 241  NLDEVDHIYGSHEAHDVGVKIE-PNIS--GFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300

Query: 301  FPTYGMQNKDNEAMDFQNNGILWLPPEPEDEEDEK-ALLFNGDDDACAPGEWGHLPSSQG 360
                  ++ + E +DF+++G+LWLPPEPE+EEDE+ A+L +            +L  S  
Sbjct: 301  GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDXXXXXXXXXXXYLRPSNS 360

Query: 361  SGECPM--KRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLS 420
              E     K KSSG      KNVVEGHFRALV+QLLE +NLP+ +E +E+GWLDI+T LS
Sbjct: 361  FNEKDFHSKDKSSG----AMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420

Query: 421  WEAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKP 480
            WEAA +LKPDTSKSG MDPGGYVKVKC+  G+R+ES++VKG++CKKNVAHRRM  +I KP
Sbjct: 421  WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            R  +LGGALEYQR++N LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQEY
Sbjct: 481  RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540

Query: 541  LLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDG 600
            LL+K I+LVLN+KRSLLERI+ CTGA IVP++D +   KLGYCD F V+ FVE H S   
Sbjct: 541  LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS-PC 600

Query: 601  QAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            Q  KK  KTLMFF+GCPKPL CTILL+GA  DELKKVKHV+QY  FAAYHLALET FLAD
Sbjct: 601  QVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAV 720
            EGAS+ ELPL++PI V LP  PS ++RSISTIPGFT  S  KSP  E   E  ++  D  
Sbjct: 661  EGASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLT 720

Query: 721  P--TRDSSMISHNVDNLNSLEPSLRLEKSNATSTSLSFLKQNVSTADRNYMLSSKLASEA 780
               T   +     +D  + ++PS RL   +   T      + +++ D   +         
Sbjct: 721  GNFTSSKTHFQGKLDGNDRIDPSERL--LHNLDTVYCKPPETITSKDDGLV--------- 780

Query: 781  NRNPDSEEYSMGIMTIKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSLDANTQSSNDST 840
               P  E   +     +   ++D  +V S         G +  +       ++    +  
Sbjct: 781  ---PTLESRQLSFHVEEPSVQKDQWSVLS---------GATEQVTDGGYTNDSAVIGNQN 840

Query: 841  CQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSF 900
              R E +       +SS+ DF  S SD Q+ILVSLSTRCVWKG++C+RAHLLRIKYYGSF
Sbjct: 841  FNRQEQM-------ESSKGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSF 900

Query: 901  DKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKI 960
            DKPLGRFLRDNLFDQ+  C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKI
Sbjct: 901  DKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKI 960

Query: 961  WMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRD 1020
            WMWHRCLKCPR NGFPPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRD
Sbjct: 961  WMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRD 1020

Query: 1021 CLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFHHNNLEWINKEANEVHNRAEVLFAVVC 1080
            CLRFYGFGR VACF YA+I++ +V +PP+ + F++ N EW+ KE+ EV  +AEVLF  V 
Sbjct: 1021 CLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQ 1080

Query: 1081 KTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHFNKASGETTVGQPTVD 1140
            + L  I     G   +    N++   ++EL  ++   K++++    +       GQPT+D
Sbjct: 1081 EALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLNVVKDGQPTID 1140

Query: 1141 IFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEEKELDSMEELVELTL 1200
            I  INKL   ++F SY W + L+ A  + + +  ++  N  P +  + + S+E+L +  +
Sbjct: 1141 ILLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKV 1200

Query: 1201 AFGHDDLFGGFDNDSLPSESKHDVDLDRGGNSGQMNRFNETEREKNI--DLGGQEDDLCL 1260
                  +     NDSL  ++ ++  L++G        F +T  +  I  D+G      C 
Sbjct: 1201 KSIPTHV--AICNDSLLQDADYETCLNQG------KSFADTSGKFAIPEDVGSDRPPDCR 1260

Query: 1261 YQVNSEP------LDVGTALRRTNSEGEYPSMTDLSGTLDAVWKGALHPNSMLFDDRYPQ 1320
             + +         ++    ++  ++E ++ + TDLS TLDA W G              Q
Sbjct: 1261 MEFDPSEGGKDNFVESSQVVKPAHTESQFQA-TDLSDTLDAAWIG-------------EQ 1320

Query: 1321 TSSSILEPLAGPDMEKCTANG--VETARLL--YSALVLTGVDSVETCTSLTSMPSSAY-- 1380
            T+S     +  P     + NG  +   RLL   S L   G  + +  T+   +PS ++  
Sbjct: 1321 TTSE--NGIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDEHTTQVQLPSPSFYY 1380

Query: 1381 -SSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPVYDEEPTSIIA 1440
              +K   LN  K  +++  P  ++S+RELE +   RL + +  +D V+PVYD+EPTSIIA
Sbjct: 1381 SLNKNYSLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIA 1440

Query: 1441 YSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVYRTLRSNEETI 1500
            Y+L   EY AQMS  ++  + LD+  S     SV + S+ +  +   D+ R+L S +E +
Sbjct: 1441 YALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQV 1500

Query: 1501 LSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEALRKKCCPSEL 1560
              +  +         L+ KDLH R+ F +E P G VKY++TCY+AK FEALR  CCPSE 
Sbjct: 1501 SQLLHSS--------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSET 1560

Query: 1561 DYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPAYFLYLSESIR 1620
            D++RSL RC++WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESFI+F PAYF YL+ESI 
Sbjct: 1561 DFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESIS 1620

Query: 1621 TGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRLYDLKGSSRSRYNSD 1680
            T  PT LAK++G+YQVS+KHLKGGKEFK+DVLVMENLLF  N TRLYDLKGS+R+RYN D
Sbjct: 1621 TKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPD 1680

Query: 1681 TSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEE 1740
            TSG N VLLDQNL+E+MPTSPIF+G KAKRLLERAVWNDTSFLASI VMDYSLLVG+DEE
Sbjct: 1681 TSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEE 1722

Query: 1741 KQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFL 1757
            + ELV+GIIDFMRQYTWDKHLETWVK SG+LGGPKN+ PTVISP QYKKRFRKAMT YFL
Sbjct: 1741 RNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFL 1722

BLAST of Carg25515 vs. TAIR10
Match: AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C)

HSP 1 Score: 1051.2 bits (2717), Expect = 7.6e-307
Identity = 615/1455 (42.27%), Postives = 859/1455 (59.04%), Query Frame = 0

Query: 330  SSQGSGECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTY 389
            SS  S   P K K   +     + VV  HFRALV++LL  E L   D+ +   WLDI+T 
Sbjct: 300  SSSFSSHIPTKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTA 359

Query: 390  LSWEAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEIS 449
            L+W+AA  +KPDT   G MDPG YVK+KCVA G + ESI+++GI+C KN+ H+RM  +  
Sbjct: 360  LAWQAANFVKPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYK 419

Query: 450  KPRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQ 509
             PR  +L G+LEYQRV   L+SF+TLLQQE +H+K  +AKI++  PNVLLVEKS S +AQ
Sbjct: 420  NPRVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQ 479

Query: 510  EYLLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSG 569
            +YLL K+I+LVLNVKRSLL+RIA CTGA + P++D ++  +LG+C+ FR +  +E+H +G
Sbjct: 480  QYLLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAG 539

Query: 570  DGQAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFL 629
            + Q+ +K ++TLM+FEGCP+ L CT++LRG+  +ELKKVKHV+QYA FAAYHL+LET FL
Sbjct: 540  N-QSNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFL 599

Query: 630  ADEGASVPELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGND 689
            ADEGAS+P++ LK P +V        ID  IS I      SP ++ + ++ +ET    ++
Sbjct: 600  ADEGASLPKIRLKQPGMVRTASQRRIIDEGISLI----TQSPTET-DSQALLETAAHEDE 659

Query: 690  ---AVPTRD-SSMISHNVDNLNSLEPSLRLEKSNATSTSLSFLKQNVSTADRNYMLSSKL 749
                +P  +    +  + D      PS  +E   + + +  F    V+   R+Y      
Sbjct: 660  HTAPMPEHEVCESLCEDFDPTQIFPPSSEVETEQSDTLNGDFANNLVT---RSY------ 719

Query: 750  ASEANRNPDSEEYSMGIMT-IKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSLDANTQS 809
               +N+  D  E ++ + + I E   +       +G+G                +   Q 
Sbjct: 720  --SSNQLNDLHEPTLCLSSEIPETPTQQPSGEEDNGRG----------------EEENQL 779

Query: 810  SNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIK 869
             N     + ES   + V    S E F  + S  Q+ILVS S+RCV K ++C+R+ LLRIK
Sbjct: 780  VNPQDLPQHESFYEDDV----SSEYFSAADS-HQSILVSFSSRCVLKESVCERSRLLRIK 839

Query: 870  YYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGE 929
            +YGSFDKPLGR+L+D+LFD+   C SC    +AHV CY+H  G+LTI+ ++LP   LPGE
Sbjct: 840  FYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVLCYSHQNGNLTINVRRLPSMKLPGE 899

Query: 930  PEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGH 989
             +GKIWMWHRCL+C   +G PPATRRV+MSDAAWGLSFGKFLELSFSN+A A+RVA CGH
Sbjct: 900  QDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCGH 959

Query: 990  FLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFH-HNNLEWINKEANEVHNRAEV 1049
             L RDCLRFYGFG  VA F Y+ I++ +V +PPS+++F+ H   EWI  EA E+  +   
Sbjct: 960  SLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMRT 1019

Query: 1050 LFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHFNKASGETTV 1109
            ++  +   L  + +  S  E E     +L++ I  L   +  EK++++        E   
Sbjct: 1020 MYTEISDMLNRMEEKSSLLEPEQSEACDLHSRIIGLIDQLVKEKDEYDDALQPIFEENLQ 1079

Query: 1110 GQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEEKELDSMEE 1169
             Q ++DI E+N+L   ++  ++ W  +L    +  KK S                     
Sbjct: 1080 IQGSLDILELNRLRRALMIGAHAWDHQLYLLNSQLKKAS--------------------- 1139

Query: 1170 LVELTLAFGHDDLFGGFDNDSLPSESKHD---VDLDRGGNSGQMNRFNETEREKNIDLGG 1229
                        +F   D+++  +   HD   +D      S + +  + T+ E N D   
Sbjct: 1140 ------------VFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSHTDSEANGD--- 1199

Query: 1230 QEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSM-TDLSGTLDAVWKGA---LHPNSMLFD 1289
                      N +P ++             PS  T LS  +D+ W G+   L     + +
Sbjct: 1200 ----------NKDPENI-------------PSPGTSLSERIDSAWLGSFQNLEKAETIAE 1259

Query: 1290 DRYPQTSSSILEPLAGPDMEKCTANGVETARLLY-----SALVLTGVDSVETCTSLTSMP 1349
                   +S L  LA P   +   + +     +      S+L L+ + S        +M 
Sbjct: 1260 TEGFSAVNSSLRRLARPIRVQSFDSAIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMV 1319

Query: 1350 SSAYS------SKISLLNIPKPD-ISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPV 1409
                S      S++  L + K D I    P  I+S  ++   G   L      +D VVPV
Sbjct: 1320 RDPVSNVMRTYSQMLPLEVQKLDLIVGSAPTYISSASQM-ADGARMLIPQRGLNDIVVPV 1379

Query: 1410 YDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVY 1469
            YD++P S+++Y++   EY   +     V + L ++ S   + +      +  +      +
Sbjct: 1380 YDDDPASVVSYAINSKEYKEWI-----VNKGLASSSSSSNLNN------RESEPSAFSTW 1439

Query: 1470 RTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEA 1529
            R+L  + + I       S         T     R    +    G VK+++TCYFA +F+ 
Sbjct: 1440 RSLSMDVDYIQHAVYGSSQDDRKSPHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDT 1499

Query: 1530 LRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPA 1589
            LRK CCPSE+D+VRSLSRC+RW AQGGKSNV+FAK+LDERFIIKQV K EL+SF  FAP 
Sbjct: 1500 LRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPE 1559

Query: 1590 YFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRLYDLK 1649
            YF YL ES+ +G PTCLAK++G+YQVS KH KGGKE K+D++VMENL +   ++R+YDLK
Sbjct: 1560 YFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLK 1619

Query: 1650 GSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDVMD 1709
            GS+RSRYN +TSG +KVLLD NL+E++ T PIFLG KAKR LERA+WNDT+FLAS+DVMD
Sbjct: 1620 GSARSRYNPNTSGADKVLLDMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMD 1645

Query: 1710 YSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKR 1760
            YSLLVG DEE++ELV+GIIDFMRQYTWDKHLETWVK SGILGGPKNA+PT++SP QYK+R
Sbjct: 1680 YSLLVGFDEERKELVLGIIDFMRQYTWDKHLETWVKASGILGGPKNASPTIVSPKQYKRR 1645

BLAST of Carg25515 vs. TAIR10
Match: AT1G34260.1 (FORMS APLOID AND BINUCLEATE CELLS 1A)

HSP 1 Score: 639.4 bits (1648), Expect = 6.9e-183
Identity = 485/1454 (33.36%), Postives = 721/1454 (49.59%), Query Frame = 0

Query: 334  SGECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDG-WLDILTYLSW 393
            S E   KRK   + RR      +  F+ +VSQL+++    +     E G W +I+  L W
Sbjct: 135  SDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSI----EESGYWFEIVARLCW 194

Query: 394  EAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPR 453
            EAA++LKP       +DP  Y+KVKC+A G   +S + KG++ KK+ A + M  +   PR
Sbjct: 195  EAASMLKPAIDGKS-VDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATKYEHPR 254

Query: 454  FFVLGGALEYQRVTNHLSSFDTLLQQE---LDHLKMAVAKIDAHHPNVLLVEKSVSRHAQ 513
              ++ G L +    +  SS  ++ Q     L ++K  V  I+A  P+V+LVEKSVSR  Q
Sbjct: 255  IMLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSVSRDIQ 314

Query: 514  EYLLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSG 573
            + +L K +TLV ++K   L+RI+ C G+ I+ +VD ++  KL +CD FR++  VEEH + 
Sbjct: 315  KTILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVEEHNAA 374

Query: 574  DGQAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFL 633
             G++ KK TKTLMF EGCP  L CTILL+G   + LKKVK VVQY+   AYHL LE  FL
Sbjct: 375  -GESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVVQYSFILAYHLMLEASFL 434

Query: 634  ADEGASVPELPLK---SPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSME-TGQ 693
            AD       +  K   S ++     SPS   R     P      PV +   E +++  G+
Sbjct: 435  ADRHTMFSTIFAKEATSCVVEIENFSPSPSPRE---SPSEAVDIPVSNGFDEQTIQINGE 494

Query: 694  SGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNATSTSLSFLKQNVSTADRNYMLSSKL 753
            +  + V T +S     + D++ S EP   +  +  +S                  LS++L
Sbjct: 495  ADGEKVGTWES-----DGDHVFSHEPYNPVIFTGFSS------------------LSARL 554

Query: 754  ASEAN--RNPDSEEYSMG-----IMTIKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSL 813
            +      +NP+S   S+         +    + ++DT   +    PL     + + SSS 
Sbjct: 555  SKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLLDPELPVNSSSD 614

Query: 814  DANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRA 873
            D + +S  ++  +                     S  + Q+ILV +S R   +G +CD+ 
Sbjct: 615  DGDNKSQTENDIE---------------------STLESQSILVLVSKRNALRGIMCDQR 674

Query: 874  HLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLP- 933
            H   IK+Y  FD PL +FLRD +F+Q   C +C    EAH++ Y H    LTI  K++P 
Sbjct: 675  HFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPV 734

Query: 934  DFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAAS 993
               L GE +GKIWMW RC KC   N    +T+RV++S AA  LSFGKFLELSFS     +
Sbjct: 735  AKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLN 794

Query: 994  RVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFH-HNNLEWINKEANE 1053
            R + CGH    D L F+G G  VA   Y+ +   +V +PP  ++        W+ KE   
Sbjct: 795  RSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQT 854

Query: 1054 VHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHF-N 1113
            V  +   LF      L  +    S     D         +  +E+++  E+  FE +  N
Sbjct: 855  VFTKGISLFEDAAGFLKRL---RSQFTNSDLRYQRARKLLSNIEELLKHERCIFEENIKN 914

Query: 1114 KASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEE 1173
                  T+   +  +  +N++   +L  + IW  RL  ++ L  +  P   S+D   + E
Sbjct: 915  SFDKAKTIDDVSHRLLRLNRMRWELLLQALIWNYRLQ-SLVLSDRLLP---SSDETKIYE 974

Query: 1174 KELDSMEEL----VELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGNSGQMNRFNETE 1233
            + L ++ E      E           GG D    P     D+ +  G + G     N+  
Sbjct: 975  QGLKTVSEAGMTRYENDNKVSDSGSNGGIDT---PLVEHKDIPI-AGASVGD----NDQM 1034

Query: 1234 REKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTLDAVWKGALH-P 1293
             E  +    +   LC    +S P                 + + ++   D      +H  
Sbjct: 1035 AESYVPEDNESQTLC----SSSP----------------DTTSPINNHFDTHLAVNVHST 1094

Query: 1294 NSMLFDDRYPQTSSSILEPLAGPDMEKCTANGV------ETARLLYSALVLTGVDSVETC 1353
            N    D   P T  S+       D E  T+NG       E   L +  L    +  +E  
Sbjct: 1095 NGQEADKSIPVTGESL-------DDEVSTSNGPHILGWDEWFWLPFEELRSKRIVDIE-- 1154

Query: 1354 TSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPV 1413
                      Y  K   +N       ++    + +  ++  +   RL + +   D +V  
Sbjct: 1155 --------KEYLLKFEYVN-------NFTQENLQTVNQIITEESSRLRISLRDDDFIVSD 1214

Query: 1414 YDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVY 1473
            Y++E +S+IA +L     H    E ++ +       S    GS++       D + +D  
Sbjct: 1215 YEDELSSLIACALA----HLNNEESKKPL-------SRCIHGSLQGFLDNNQDSKQTDRD 1274

Query: 1474 RTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEA 1533
             +  S+E T     R E+L   ++L         V F +   +G  KY+I   +A  F  
Sbjct: 1275 VSRFSSEST----NRLETLPPPEVL---------VTFGSVKSVGKPKYSIVSLYADDFRD 1334

Query: 1534 LRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPA 1593
            LRK+CC SELDY+ SLSRCK W A+GGKS   FAKTLD+RFI+K++ K E ESF+ FA  
Sbjct: 1335 LRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATE 1394

Query: 1594 YFLYLSESIRTGCPTCLAKVVGMYQVSTKHLK-GGKEFKIDVLVMENLLFGHNVTRLYDL 1653
            YF Y+ +S   G  TCLAKV+G++QV+ +  K GGKE + D++VMENL F   VTR YDL
Sbjct: 1395 YFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDL 1446

Query: 1654 KGSSRSRYN-SDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDV 1713
            KG+  +R+  +  +G++ VLLDQN +  M  SP+++   +K+ L+RAV+NDTSFL SI+V
Sbjct: 1455 KGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINV 1446

Query: 1714 MDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYK 1757
            MDYSLLVG+D+E  ELV GIID++RQYTWDK LETWVK+S ++  PKN  PTVISP  YK
Sbjct: 1515 MDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSSLVV--PKNVQPTVISPIDYK 1446

BLAST of Carg25515 vs. TAIR10
Match: AT5G26360.1 (TCP-1/cpn60 chaperonin family protein)

HSP 1 Score: 78.6 bits (192), Expect = 4.7e-14
Identity = 66/253 (26.09%), Postives = 119/253 (47.04%), Query Frame = 0

Query: 413 YVKVKCVAGGKRTESIIVKGIICKKN-VAHRRMRKEISKPRFFVLGGALEYQRVTNHLSS 472
           Y+KV+ V GG+  +S ++KG++  K+ VA  +M+++I  PR  +L   LEY++  N  ++
Sbjct: 197 YIKVEKVPGGQFEDSEVLKGVMFNKDVVAPGKMKRKIVNPRIILLDCPLEYKKGENQTNA 256

Query: 473 -------FDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVK 532
                  ++ LL+ E ++++    +I    P++++ EK +S  A  Y     ++ +  ++
Sbjct: 257 ELVREEDWEVLLKLEEEYIENICVQILKFKPDLVITEKGLSDLACHYFSKAGVSAIRRLR 316

Query: 533 RSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFF 592
           ++   RIA   GA IV   D +    +G              G+G  +  K       F 
Sbjct: 317 KTDNNRIAKACGAVIVNRPDELQESDIG-------------TGAGLFEVKKIGDDFFSFI 376

Query: 593 EGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGAS----VPELP 652
             C +P  CT+LLRG S D + +V+  +Q A   A ++      +   GA+       L 
Sbjct: 377 VDCKEPKACTVLLRGPSKDFINEVERNLQDAMSVARNIIKNPKLVPGGGATELTVSATLK 436

Query: 653 LKSPIIVGLPHSP 654
            KS  I G+   P
Sbjct: 437 QKSATIEGIEKWP 436

BLAST of Carg25515 vs. Swiss-Prot
Match: sp|Q9LUM0|FAB1B_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 1597.8 bits (4136), Expect = 0.0e+00
Identity = 919/1806 (50.89%), Postives = 1164/1806 (64.45%), Query Frame = 0

Query: 9    SDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCSK 68
            S++VGL+KSW+PWRS+P  VSRDFWMPD+SCRVCY+CD QFT INRRHHCR CGRVFC K
Sbjct: 12   SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69   CTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDK--ATCSAISLSSSST 128
            CTAN IP   +D    RED E IRVCNYCF+QW +      DG    +  + +S S S T
Sbjct: 72   CTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQG-----DGGPHVSNITELSTSPSET 131

Query: 129  SIGSTKSGYTCQS---AISNIPSTPCSNDRQYY--DPFCSGSIADEQDSLRNGKTIKRST 188
            S+ S+K+  T  S   A+ ++P     N R ++  D    G  + E    + GK   R +
Sbjct: 132  SLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRS 191

Query: 189  SLMTS-----SSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLE 248
            S + +     S       RSDDE                H P+++ YY       G+ ++
Sbjct: 192  SFIATDVEDPSRFALNSIRSDDE--YDEYGAYQTDIETSHSPRANEYY-GPMEYNGMGID 251

Query: 249  -----------RENMSTKGSPVNFDTQSP--RGDEESSEDSDENDKGSECDFPTYGMQNK 308
                        +  S  GSP+         R   E  +   E+D   EC+  +    + 
Sbjct: 252  DVPCKHLGGETADQKSLSGSPLIHQCLESLIREGSEQFQKKSEHDGRDECEASSPADISD 311

Query: 309  DN--EAMDFQNNGILWLPPEPEDEEDEK--ALLFNGDDDACAPGEWGHL--PSSQGSGEC 368
            D   E +DF+NNG+LW+PPEPE+EEDE+  AL    D++  A GEWG+L   +S GSGE 
Sbjct: 312  DQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSGEY 371

Query: 369  PMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAV 428
              + +++ ++++  KNVV+GHFRAL++QLL+ EN+ V DE  ++ WL+I+T LSWEAA +
Sbjct: 372  RGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANL 431

Query: 429  LKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLG 488
            LKPD SKSG MDPGGYVKVKC+A G R +S++VKG++CKKNV +RRM  +I K R  +LG
Sbjct: 432  LKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILG 491

Query: 489  GALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKI 548
            G LEYQRV+N LSSFDTLLQQE DHLKMAVAKI A  PN+LLVEKSVSR AQEYLL+K I
Sbjct: 492  GGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDI 551

Query: 549  TLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKS 608
            +LVLN+KR LL+RIA CTGA I+P+VDH++  KLGYC+ FRV  + EEHGS  GQ GKK 
Sbjct: 552  SLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGS-TGQVGKKV 611

Query: 609  TKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGASVP 668
             KTLM+FE CPKPL  TILLRGA+ DELKKVKHVVQY  FAAYHLALET FLADEGAS P
Sbjct: 612  VKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS-P 671

Query: 669  ELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSS 728
            ELPL SPI V LP   ++I+RSIST+PGFT  +  KSP   S  E  ++  ++VP  +  
Sbjct: 672  ELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRA--NSVPVSELL 731

Query: 729  MISHNVDNLNSLEPSLRLE--KSNATSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDS 788
              + N+     + P       ++   + S  F + N+S    + ++ S+ +  + R+   
Sbjct: 732  STTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSGRSVPV 791

Query: 789  EEYSMGIMTIKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSLDANTQSSNDSTCQRPES 848
            +  +     I    +  ++++  SG+G       S  I +S +  N  +           
Sbjct: 792  DTPADKSNPIVVADETTNNSLHLSGQGFV---RKSSQIGTSIMVENQDNGXXXXXXXXXX 851

Query: 849  LIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGR 908
                           P SPSD Q+ILVSLS+R VWKGT+C+R+HL RIKYYGSFDKPLGR
Sbjct: 852  XXXXXXXXXXXXXXXP-SPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGR 911

Query: 909  FLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRC 968
            FLRD+LFDQ+YRC SC MPSEAHVHCYTH QGSLTIS KKL D+ LPGE EGKIWMWHRC
Sbjct: 912  FLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRC 971

Query: 969  LKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYG 1028
            L+CPR NGFPPAT RV+MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYG
Sbjct: 972  LRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYG 1031

Query: 1029 FGRTVACFHYAAIDVKSVHIPPSIIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLI 1088
            FG  VACF YA IDV SV++PPSI+ F++ N +WI +E +EV  RAE+LF+ V   +  I
Sbjct: 1032 FGNMVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQI 1091

Query: 1089 LKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHFNK-ASGETTVGQPTVDIFEIN 1148
               E G  +           I ELE+++  EK +FE +  K    E   GQP VDI E+ 
Sbjct: 1092 A--EKGFRRR----------IGELEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELY 1151

Query: 1149 KLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHD 1208
            ++H  +LF SY+W  RL  A TL K  + D +  +    E+  L   + L E+    G +
Sbjct: 1152 RIHRQLLFQSYMWDHRLINASTLHKLENSDDTKRE--ENEKPPLAKSQTLPEMNA--GTN 1211

Query: 1209 DLFGGFDNDSLPSESKHDVDLDRGGNSGQMNRFNETEREKNIDL------GGQEDDLCLY 1268
             L  G       SE   + D D  G++G +N   + E + N DL      GG+       
Sbjct: 1212 SLLTG-------SEVNLNPDGDSTGDTGSLNNV-QKEADTNSDLYQEKDDGGEVSPSKTL 1271

Query: 1269 QVNSEPLDVGTALRRTNSEGEYPSMTDLSGTLDAVWKG--------------ALHPNSML 1328
               S PL+    +RRT S+G+   M +LS TLDA W G              +L P++M 
Sbjct: 1272 PDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTMS 1331

Query: 1329 FDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDSVETCTSLTSMPSSA 1388
                +P  S  ++ P+  P+ +    N  + A  +  AL     ++ E   S  S+P   
Sbjct: 1332 NSSTFPPISEGLM-PIDLPEQQ----NEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLN 1391

Query: 1389 YSSKIS---LLNIPKPD-ISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPVYDEEPT 1448
            +   I+   LL+  K D   ++ P  I+SFRE E QG  RL + V  +D VVPVYD+EPT
Sbjct: 1392 FYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPT 1451

Query: 1449 SIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVYRTLRSN 1508
            S+IAY+L+ PEY  Q S     + +  +  ++P          +  D+   D  R+  S 
Sbjct: 1452 SMIAYALMSPEYQRQTSAEGESLVSYPSELNIP----------RPVDDTIFDPSRSNGSV 1511

Query: 1509 EETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEALRKKCC 1568
            +E+ILS++ + S    D L +TK LH RV +  +  LG VKYT+TCY+AKRFEALR  C 
Sbjct: 1512 DESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICL 1571

Query: 1569 PSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPAYFLYLS 1628
            PSEL+Y+RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+FAPAYF YLS
Sbjct: 1572 PSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLS 1631

Query: 1629 ESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRLYDLKGSSRSR 1688
            ESI T  PTCLAK++G+YQV+TK LK GKE K+DVL+MENLLFG  V RLYDLKGSSR+R
Sbjct: 1632 ESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRAR 1691

Query: 1689 YNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDVMDYSLLVG 1748
            YN D+SG NKVLLDQNLIE+MPTSPIF+G KAKRLLERAVWNDT+FLA  DVMDYSLLVG
Sbjct: 1692 YNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVG 1751

Query: 1749 LDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMT 1757
            +DEEK ELV+GIIDF+RQYTWDKHLE+WVK +GILGGPKN APTVISP QYK+RFRKAMT
Sbjct: 1752 VDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYKRRFRKAMT 1761

BLAST of Carg25515 vs. Swiss-Prot
Match: sp|Q0WUR5|FAB1A_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 1529.2 bits (3958), Expect = 0.0e+00
Identity = 875/1802 (48.56%), Postives = 1131/1802 (62.76%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P +S+  N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFC+KC AN IPSPS +  ++ E+ E IRVCNYC+KQW +      +G        S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
             S S+ S+ ST S             T                                 
Sbjct: 121  SSPSARSVASTTS--------XXXXCTIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXR------------------------- 240
                  SS +YG+     ++          XXXX                          
Sbjct: 181  XXXXRRSSDHYGHVLDSSDNQVEFFVNSSGXXXXXXXXXXXXXXXXXXXXXXGNDYYGAI 240

Query: 241  HIPQSDHYYCSANTEE-GLNLERENMSTKGSPVNFDTQSPRGDEESSEDSDENDKGSECD 300
            ++ + DH Y S    + G+ +E  N+S  G P + D  S   +        EN      +
Sbjct: 241  NLDEVDHIYGSHEAHDVGVKIE-PNIS--GFPPDQDLDSLNTETIDKTRQQENGWNDVKE 300

Query: 301  FPTYGMQNKDNEAMDFQNNGILWLPPEPEDEEDEK-ALLFNGDDDACAPGEWGHLPSSQG 360
                  ++ + E +DF+++G+LWLPPEPE+EEDE+ A+L +            +L  S  
Sbjct: 301  GSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDXXXXXXXXXXXYLRPSNS 360

Query: 361  SGECPM--KRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLS 420
              E     K KSSG      KNVVEGHFRALV+QLLE +NLP+ +E +E+GWLDI+T LS
Sbjct: 361  FNEKDFHSKDKSSG----AMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLS 420

Query: 421  WEAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKP 480
            WEAA +LKPDTSKSG MDPGGYVKVKC+  G+R+ES++VKG++CKKNVAHRRM  +I KP
Sbjct: 421  WEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            R  +LGGALEYQR++N LSSFDTLLQQE+DHLKMAVAKID+H+P++LLVEKSVSR AQEY
Sbjct: 481  RLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEY 540

Query: 541  LLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDG 600
            LL+K I+LVLN+KRSLLERI+ CTGA IVP++D +   KLGYCD F V+ FVE H S   
Sbjct: 541  LLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS-PC 600

Query: 601  QAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            Q  KK  KTLMFF+GCPKPL CTILL+GA  DELKKVKHV+QY  FAAYHLALET FLAD
Sbjct: 601  QVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAV 720
            EGAS+ ELPL++PI V LP  PS ++RSISTIPGFT  S  KSP  E   E  ++  D  
Sbjct: 661  EGASIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLT 720

Query: 721  P--TRDSSMISHNVDNLNSLEPSLRLEKSNATSTSLSFLKQNVSTADRNYMLSSKLASEA 780
               T   +     +D  + ++PS RL   +   T      + +++ D   +         
Sbjct: 721  GNFTSSKTHFQGKLDGNDRIDPSERL--LHNLDTVYCKPPETITSKDDGLV--------- 780

Query: 781  NRNPDSEEYSMGIMTIKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSLDANTQSSNDST 840
               P  E   +     +   ++D  +V S         G +  +       ++    +  
Sbjct: 781  ---PTLESRQLSFHVEEPSVQKDQWSVLS---------GATEQVTDGGYTNDSAVIGNQN 840

Query: 841  CQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSF 900
              R E +       +SS+ DF  S SD Q+ILVSLSTRCVWKG++C+RAHLLRIKYYGSF
Sbjct: 841  FNRQEQM-------ESSKGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSF 900

Query: 901  DKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGEPEGKI 960
            DKPLGRFLRDNLFDQ+  C SC MP+EAH+HCYTH QGSLTIS KKLP+  LPG+ EGKI
Sbjct: 901  DKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKI 960

Query: 961  WMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRD 1020
            WMWHRCLKCPR NGFPPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRD
Sbjct: 961  WMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRD 1020

Query: 1021 CLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFHHNNLEWINKEANEVHNRAEVLFAVVC 1080
            CLRFYGFGR VACF YA+I++ +V +PP+ + F++ N EW+ KE+ EV  +AEVLF  V 
Sbjct: 1021 CLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQ 1080

Query: 1081 KTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHFNKASGETTVGQPTVD 1140
            + L  I     G   +    N++   ++EL  ++   K++++    +       GQPT+D
Sbjct: 1081 EALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLNVVKDGQPTID 1140

Query: 1141 IFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEEKELDSMEELVELTL 1200
            I  INKL   ++F SY W + L+ A  + + +  ++  N  P +  + + S+E+L +  +
Sbjct: 1141 ILLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNV-SLEKLSDEKV 1200

Query: 1201 AFGHDDLFGGFDNDSLPSESKHDVDLDRGGNSGQMNRFNETEREKNI--DLGGQEDDLCL 1260
                  +     NDSL  ++ ++  L++G        F +T  +  I  D+G      C 
Sbjct: 1201 KSIPTHV--AICNDSLLQDADYETCLNQG------KSFADTSGKFAIPEDVGSDRPPDCR 1260

Query: 1261 YQVNSEP------LDVGTALRRTNSEGEYPSMTDLSGTLDAVWKGALHPNSMLFDDRYPQ 1320
             + +         ++    ++  ++E ++ + TDLS TLDA W G              Q
Sbjct: 1261 MEFDPSEGGKDNFVESSQVVKPAHTESQFQA-TDLSDTLDAAWIG-------------EQ 1320

Query: 1321 TSSSILEPLAGPDMEKCTANG--VETARLL--YSALVLTGVDSVETCTSLTSMPSSAY-- 1380
            T+S     +  P     + NG  +   RLL   S L   G  + +  T+   +PS ++  
Sbjct: 1321 TTSE--NGIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDEHTTQVQLPSPSFYY 1380

Query: 1381 -SSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPVYDEEPTSIIA 1440
              +K   LN  K  +++  P  ++S+RELE +   RL + +  +D V+PVYD+EPTSIIA
Sbjct: 1381 SLNKNYSLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIA 1440

Query: 1441 YSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVYRTLRSNEETI 1500
            Y+L   EY AQMS  ++  + LD+  S     SV + S+ +  +   D+ R+L S +E +
Sbjct: 1441 YALTSSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQV 1500

Query: 1501 LSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEALRKKCCPSEL 1560
              +  +         L+ KDLH R+ F +E P G VKY++TCY+AK FEALR  CCPSE 
Sbjct: 1501 SQLLHSS--------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSET 1560

Query: 1561 DYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPAYFLYLSESIR 1620
            D++RSL RC++WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESFI+F PAYF YL+ESI 
Sbjct: 1561 DFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESIS 1620

Query: 1621 TGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRLYDLKGSSRSRYNSD 1680
            T  PT LAK++G+YQVS+KHLKGGKEFK+DVLVMENLLF  N TRLYDLKGS+R+RYN D
Sbjct: 1621 TKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPD 1680

Query: 1681 TSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEE 1740
            TSG N VLLDQNL+E+MPTSPIF+G KAKRLLERAVWNDTSFLASI VMDYSLLVG+DEE
Sbjct: 1681 TSGSNTVLLDQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEE 1722

Query: 1741 KQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFL 1757
            + ELV+GIIDFMRQYTWDKHLETWVK SG+LGGPKN+ PTVISP QYKKRFRKAMT YFL
Sbjct: 1741 RNELVLGIIDFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFL 1722

BLAST of Carg25515 vs. Swiss-Prot
Match: sp|Q9SSJ8|FAB1C_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.4e-305
Identity = 615/1455 (42.27%), Postives = 859/1455 (59.04%), Query Frame = 0

Query: 330  SSQGSGECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTY 389
            SS  S   P K K   +     + VV  HFRALV++LL  E L   D+ +   WLDI+T 
Sbjct: 300  SSSFSSHIPTKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTA 359

Query: 390  LSWEAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEIS 449
            L+W+AA  +KPDT   G MDPG YVK+KCVA G + ESI+++GI+C KN+ H+RM  +  
Sbjct: 360  LAWQAANFVKPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYK 419

Query: 450  KPRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQ 509
             PR  +L G+LEYQRV   L+SF+TLLQQE +H+K  +AKI++  PNVLLVEKS S +AQ
Sbjct: 420  NPRVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQ 479

Query: 510  EYLLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSG 569
            +YLL K+I+LVLNVKRSLL+RIA CTGA + P++D ++  +LG+C+ FR +  +E+H +G
Sbjct: 480  QYLLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAG 539

Query: 570  DGQAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFL 629
            + Q+ +K ++TLM+FEGCP+ L CT++LRG+  +ELKKVKHV+QYA FAAYHL+LET FL
Sbjct: 540  N-QSNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFL 599

Query: 630  ADEGASVPELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGND 689
            ADEGAS+P++ LK P +V        ID  IS I      SP ++ + ++ +ET    ++
Sbjct: 600  ADEGASLPKIRLKQPGMVRTASQRRIIDEGISLI----TQSPTET-DSQALLETAAHEDE 659

Query: 690  ---AVPTRD-SSMISHNVDNLNSLEPSLRLEKSNATSTSLSFLKQNVSTADRNYMLSSKL 749
                +P  +    +  + D      PS  +E   + + +  F    V+   R+Y      
Sbjct: 660  HTAPMPEHEVCESLCEDFDPTQIFPPSSEVETEQSDTLNGDFANNLVT---RSY------ 719

Query: 750  ASEANRNPDSEEYSMGIMT-IKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSLDANTQS 809
               +N+  D  E ++ + + I E   +       +G+G                +   Q 
Sbjct: 720  --SSNQLNDLHEPTLCLSSEIPETPTQQPSGEEDNGRG----------------EEENQL 779

Query: 810  SNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIK 869
             N     + ES   + V    S E F  + S  Q+ILVS S+RCV K ++C+R+ LLRIK
Sbjct: 780  VNPQDLPQHESFYEDDV----SSEYFSAADS-HQSILVSFSSRCVLKESVCERSRLLRIK 839

Query: 870  YYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFLPGE 929
            +YGSFDKPLGR+L+D+LFD+   C SC    +AHV CY+H  G+LTI+ ++LP   LPGE
Sbjct: 840  FYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVLCYSHQNGNLTINVRRLPSMKLPGE 899

Query: 930  PEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGH 989
             +GKIWMWHRCL+C   +G PPATRRV+MSDAAWGLSFGKFLELSFSN+A A+RVA CGH
Sbjct: 900  QDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCGH 959

Query: 990  FLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFH-HNNLEWINKEANEVHNRAEV 1049
             L RDCLRFYGFG  VA F Y+ I++ +V +PPS+++F+ H   EWI  EA E+  +   
Sbjct: 960  SLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMRT 1019

Query: 1050 LFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHFNKASGETTV 1109
            ++  +   L  + +  S  E E     +L++ I  L   +  EK++++        E   
Sbjct: 1020 MYTEISDMLNRMEEKSSLLEPEQSEACDLHSRIIGLIDQLVKEKDEYDDALQPIFEENLQ 1079

Query: 1110 GQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEEKELDSMEE 1169
             Q ++DI E+N+L   ++  ++ W  +L    +  KK S                     
Sbjct: 1080 IQGSLDILELNRLRRALMIGAHAWDHQLYLLNSQLKKAS--------------------- 1139

Query: 1170 LVELTLAFGHDDLFGGFDNDSLPSESKHD---VDLDRGGNSGQMNRFNETEREKNIDLGG 1229
                        +F   D+++  +   HD   +D      S + +  + T+ E N D   
Sbjct: 1140 ------------VFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSHTDSEANGD--- 1199

Query: 1230 QEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSM-TDLSGTLDAVWKGA---LHPNSMLFD 1289
                      N +P ++             PS  T LS  +D+ W G+   L     + +
Sbjct: 1200 ----------NKDPENI-------------PSPGTSLSERIDSAWLGSFQNLEKAETIAE 1259

Query: 1290 DRYPQTSSSILEPLAGPDMEKCTANGVETARLLY-----SALVLTGVDSVETCTSLTSMP 1349
                   +S L  LA P   +   + +     +      S+L L+ + S        +M 
Sbjct: 1260 TEGFSAVNSSLRRLARPIRVQSFDSAIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMV 1319

Query: 1350 SSAYS------SKISLLNIPKPD-ISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPV 1409
                S      S++  L + K D I    P  I+S  ++   G   L      +D VVPV
Sbjct: 1320 RDPVSNVMRTYSQMLPLEVQKLDLIVGSAPTYISSASQM-ADGARMLIPQRGLNDIVVPV 1379

Query: 1410 YDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVY 1469
            YD++P S+++Y++   EY   +     V + L ++ S   + +      +  +      +
Sbjct: 1380 YDDDPASVVSYAINSKEYKEWI-----VNKGLASSSSSSNLNN------RESEPSAFSTW 1439

Query: 1470 RTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEA 1529
            R+L  + + I       S         T     R    +    G VK+++TCYFA +F+ 
Sbjct: 1440 RSLSMDVDYIQHAVYGSSQDDRKSPHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDT 1499

Query: 1530 LRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPA 1589
            LRK CCPSE+D+VRSLSRC+RW AQGGKSNV+FAK+LDERFIIKQV K EL+SF  FAP 
Sbjct: 1500 LRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPE 1559

Query: 1590 YFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRLYDLK 1649
            YF YL ES+ +G PTCLAK++G+YQVS KH KGGKE K+D++VMENL +   ++R+YDLK
Sbjct: 1560 YFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLK 1619

Query: 1650 GSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDVMD 1709
            GS+RSRYN +TSG +KVLLD NL+E++ T PIFLG KAKR LERA+WNDT+FLAS+DVMD
Sbjct: 1620 GSARSRYNPNTSGADKVLLDMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMD 1645

Query: 1710 YSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKR 1760
            YSLLVG DEE++ELV+GIIDFMRQYTWDKHLETWVK SGILGGPKNA+PT++SP QYK+R
Sbjct: 1680 YSLLVGFDEERKELVLGIIDFMRQYTWDKHLETWVKASGILGGPKNASPTIVSPKQYKRR 1645

BLAST of Carg25515 vs. Swiss-Prot
Match: sp|Q9XID0|FAB1D_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 1.2e-181
Identity = 485/1454 (33.36%), Postives = 721/1454 (49.59%), Query Frame = 0

Query: 334  SGECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDG-WLDILTYLSW 393
            S E   KRK   + RR      +  F+ +VSQL+++    +     E G W +I+  L W
Sbjct: 135  SDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSI----EESGYWFEIVARLCW 194

Query: 394  EAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPR 453
            EAA++LKP       +DP  Y+KVKC+A G   +S + KG++ KK+ A + M  +   PR
Sbjct: 195  EAASMLKPAIDGKS-VDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATKYEHPR 254

Query: 454  FFVLGGALEYQRVTNHLSSFDTLLQQE---LDHLKMAVAKIDAHHPNVLLVEKSVSRHAQ 513
              ++ G L +    +  SS  ++ Q     L ++K  V  I+A  P+V+LVEKSVSR  Q
Sbjct: 255  IMLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSVSRDIQ 314

Query: 514  EYLLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSG 573
            + +L K +TLV ++K   L+RI+ C G+ I+ +VD ++  KL +CD FR++  VEEH + 
Sbjct: 315  KTILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVEEHNAA 374

Query: 574  DGQAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFL 633
             G++ KK TKTLMF EGCP  L CTILL+G   + LKKVK VVQY+   AYHL LE  FL
Sbjct: 375  -GESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVVQYSFILAYHLMLEASFL 434

Query: 634  ADEGASVPELPLK---SPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSME-TGQ 693
            AD       +  K   S ++     SPS   R     P      PV +   E +++  G+
Sbjct: 435  ADRHTMFSTIFAKEATSCVVEIENFSPSPSPRE---SPSEAVDIPVSNGFDEQTIQINGE 494

Query: 694  SGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNATSTSLSFLKQNVSTADRNYMLSSKL 753
            +  + V T +S     + D++ S EP   +  +  +S                  LS++L
Sbjct: 495  ADGEKVGTWES-----DGDHVFSHEPYNPVIFTGFSS------------------LSARL 554

Query: 754  ASEAN--RNPDSEEYSMG-----IMTIKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSL 813
            +      +NP+S   S+         +    + ++DT   +    PL     + + SSS 
Sbjct: 555  SKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLLDPELPVNSSSD 614

Query: 814  DANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRA 873
            D + +S  ++  +                     S  + Q+ILV +S R   +G +CD+ 
Sbjct: 615  DGDNKSQTENDIE---------------------STLESQSILVLVSKRNALRGIMCDQR 674

Query: 874  HLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLP- 933
            H   IK+Y  FD PL +FLRD +F+Q   C +C    EAH++ Y H    LTI  K++P 
Sbjct: 675  HFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPV 734

Query: 934  DFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAAS 993
               L GE +GKIWMW RC KC   N    +T+RV++S AA  LSFGKFLELSFS     +
Sbjct: 735  AKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLN 794

Query: 994  RVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFH-HNNLEWINKEANE 1053
            R + CGH    D L F+G G  VA   Y+ +   +V +PP  ++        W+ KE   
Sbjct: 795  RSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQT 854

Query: 1054 VHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVHF-N 1113
            V  +   LF      L  +    S     D         +  +E+++  E+  FE +  N
Sbjct: 855  VFTKGISLFEDAAGFLKRL---RSQFTNSDLRYQRARKLLSNIEELLKHERCIFEENIKN 914

Query: 1114 KASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEE 1173
                  T+   +  +  +N++   +L  + IW  RL  ++ L  +  P   S+D   + E
Sbjct: 915  SFDKAKTIDDVSHRLLRLNRMRWELLLQALIWNYRLQ-SLVLSDRLLP---SSDETKIYE 974

Query: 1174 KELDSMEEL----VELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGNSGQMNRFNETE 1233
            + L ++ E      E           GG D    P     D+ +  G + G     N+  
Sbjct: 975  QGLKTVSEAGMTRYENDNKVSDSGSNGGIDT---PLVEHKDIPI-AGASVGD----NDQM 1034

Query: 1234 REKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTLDAVWKGALH-P 1293
             E  +    +   LC    +S P                 + + ++   D      +H  
Sbjct: 1035 AESYVPEDNESQTLC----SSSP----------------DTTSPINNHFDTHLAVNVHST 1094

Query: 1294 NSMLFDDRYPQTSSSILEPLAGPDMEKCTANGV------ETARLLYSALVLTGVDSVETC 1353
            N    D   P T  S+       D E  T+NG       E   L +  L    +  +E  
Sbjct: 1095 NGQEADKSIPVTGESL-------DDEVSTSNGPHILGWDEWFWLPFEELRSKRIVDIE-- 1154

Query: 1354 TSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVPV 1413
                      Y  K   +N       ++    + +  ++  +   RL + +   D +V  
Sbjct: 1155 --------KEYLLKFEYVN-------NFTQENLQTVNQIITEESSRLRISLRDDDFIVSD 1214

Query: 1414 YDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDVY 1473
            Y++E +S+IA +L     H    E ++ +       S    GS++       D + +D  
Sbjct: 1215 YEDELSSLIACALA----HLNNEESKKPL-------SRCIHGSLQGFLDNNQDSKQTDRD 1274

Query: 1474 RTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFEA 1533
             +  S+E T     R E+L   ++L         V F +   +G  KY+I   +A  F  
Sbjct: 1275 VSRFSSEST----NRLETLPPPEVL---------VTFGSVKSVGKPKYSIVSLYADDFRD 1334

Query: 1534 LRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAPA 1593
            LRK+CC SELDY+ SLSRCK W A+GGKS   FAKTLD+RFI+K++ K E ESF+ FA  
Sbjct: 1335 LRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATE 1394

Query: 1594 YFLYLSESIRTGCPTCLAKVVGMYQVSTKHLK-GGKEFKIDVLVMENLLFGHNVTRLYDL 1653
            YF Y+ +S   G  TCLAKV+G++QV+ +  K GGKE + D++VMENL F   VTR YDL
Sbjct: 1395 YFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDL 1446

Query: 1654 KGSSRSRYN-SDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDV 1713
            KG+  +R+  +  +G++ VLLDQN +  M  SP+++   +K+ L+RAV+NDTSFL SI+V
Sbjct: 1455 KGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINV 1446

Query: 1714 MDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYK 1757
            MDYSLLVG+D+E  ELV GIID++RQYTWDK LETWVK+S ++  PKN  PTVISP  YK
Sbjct: 1515 MDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSSLVV--PKNVQPTVISPIDYK 1446

BLAST of Carg25515 vs. Swiss-Prot
Match: sp|Q9Z1T6|FYV1_MOUSE (1-phosphatidylinositol 3-phosphate 5-kinase OS=Mus musculus OX=10090 GN=Pikfyve PE=1 SV=3)

HSP 1 Score: 413.3 bits (1061), Expect = 1.5e-113
Identity = 510/2114 (24.12%), Postives = 825/2114 (39.03%), Query Frame = 0

Query: 8    LSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCS 67
            LS ++  +K  M  +S   ++ + +WMPD  C+ CYDC  +FT   RRHHCR CG++FCS
Sbjct: 131  LSTVLKRLKEIMEGKSQDSDL-KQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCS 190

Query: 68   KCTANFIPSPSTDPTNAREDSETIRVCNYCFK------------QWGKEPSALLDGDKAT 127
            +C    IP      T        +R C YC K              G++ +AL D   +T
Sbjct: 191  RCCNQEIPGKFMGYTG------DLRACTYCRKIALSYAHSTDSNSIGEDLNALSD---ST 250

Query: 128  CSAISLSSSS--TSIGSTKSG--------YTCQSAISNIPSTPCSNDRQY-------YDP 187
            CS   L  S   T +GS K+            QS I    S    + R           P
Sbjct: 251  CSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSP 310

Query: 188  FCSGSIADEQDSL-RNGKTIKRSTSLMTSSSSYYGYCRSDD---------EDGXXXXXXX 247
              + S +    SL R+G  +  S     S  +   Y R++          +         
Sbjct: 311  ARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWK 370

Query: 248  XXXXXXRHIPQSDHYY-------------------------------------------- 307
                    +   DH Y                                            
Sbjct: 371  KICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLD 430

Query: 308  CSANTEEGLN--------LERENMSTKGSPVNFDTQSPRGDEESS-------EDSDENDK 367
            C ++ ++           L+    S   SP +    S  G  E S       +DSD    
Sbjct: 431  CVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQI 490

Query: 368  GSECD--FPTYGMQNKDNEAMDFQN--------------------------NGILWLPPE 427
              E D         +K      FQ+                          +    +PP 
Sbjct: 491  AEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPH 550

Query: 428  PEDEEDE-----------------KALLFN------GDDDACAPGEWGH----LPSSQGS 487
            P D+++                  K  LFN        +    P  W H    L   +  
Sbjct: 551  PADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENE 610

Query: 488  GECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEA 547
             +  M+R  S ++          H  AL+ QLL+ E+L          W DI+  L  + 
Sbjct: 611  EKQAMERLLSANH---------NHMMALLQQLLQNESL-------SSSWRDIIVSLVCQV 670

Query: 548  AAVLKPDTS-KSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPRF 607
               ++PD   +   MD   +V +K + GGK+ +S++V G +C KN+AH++M   I  P+ 
Sbjct: 671  VQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKI 730

Query: 608  FVLGGALEY-QRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYL 667
             +L  ++EY  R     +  D ++ QE + LK  V +I    P ++LVEK+VSR AQ+ L
Sbjct: 731  LLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDML 790

Query: 668  LSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACL-KLGYCDEFRVKTFVEEHGSGDG 727
            L   ITLV+NVK  +LERI+  T  ++V ++D +     LG C +F ++ F         
Sbjct: 791  LEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIF--------- 850

Query: 728  QAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 787
            Q   + TKTLMFFEGCP+ L CTI LRG S  EL +VK ++ +    AYH  LE  FL D
Sbjct: 851  QLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMD 910

Query: 788  E-------------------------------GASVPELP--------------LKSPII 847
            E                               G+S+P+ P              L+S  +
Sbjct: 911  EFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSEDSTLLESRTV 970

Query: 848  V--------GLPHSPSNIDRSISTIPGFTCPS-----------------------PVKSP 907
            +         +P + +++     T P  TCP+                        V + 
Sbjct: 971  LEKGELDNKSIPQAVASLKHQDYTTP--TCPAGIPCALFALVPESLLPLHMDQQDAVGNE 1030

Query: 908  EPESSMETGQSGN---------DAVPTRDSSMISHNVDN--------------------- 967
            +PE+S +T +  +         D +       ++  V +                     
Sbjct: 1031 QPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVIL 1090

Query: 968  -----LNSLEPSLRLEKSNATSTSLSFLKQ-----------NVSTADRNYMLSSKLASEA 1027
                 +   EP L  EK    ST   F +Q               + R   L   L+   
Sbjct: 1091 CISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVKEMESRRKKQLLRDLSGLQ 1150

Query: 1028 NRNPDSEEYSMGIMTIKE-------EAKEDDDTVS-------SSGKGVPLSHGNS-IHIR 1087
              N   +  S+ ++   E       E   D  T+        + G  +   H +  +H +
Sbjct: 1151 GMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSK 1210

Query: 1088 SSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKG-- 1147
             +S  +  +S N +       LI + V   +  +   L+P++ Q + V  S+        
Sbjct: 1211 DASCTSGGKSGNKTESDEERGLIPSDVIWPTKVD--CLNPANHQRLCVLFSSSSAQSSNA 1270

Query: 1148 -TICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHS--CGMPSEAHVHCYTHPQGSL 1207
             + C    ++ +++YG  D  LG FL    F  +Y+C S  C  P   H+  + H QG +
Sbjct: 1271 PSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCV 1330

Query: 1208 TISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELS 1267
             I  K+L D  +PG  +  I  +  C  C +       T  V +S+ +W +SF K+LEL 
Sbjct: 1331 QIILKEL-DSPVPGY-QHTILTYSWCRICKQ------VTPVVALSNESWSMSFAKYLELR 1390

Query: 1268 FSNNAAASRVAG--CGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIP-PSIIDFHHNN 1327
            F  +    R     CGH +H D  +++ + + VA F Y+ I +  V +P P I       
Sbjct: 1391 FYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKI------- 1450

Query: 1328 LEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVE 1387
              +I ++A                     LK     + +DF       Y+   E++ +++
Sbjct: 1451 --FIKRQAP--------------------LKVSLLQDLKDFFQKVSQVYLAVDERLASLK 1510

Query: 1388 KEDFEVHFNKASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSS 1447
             + F           T  +   DIF   ++          W +++   +      +P   
Sbjct: 1511 TDTF---------SKTREEKMEDIFAQKEMEEGEF---KNWTEKMQARLMSSSVDTP--- 1570

Query: 1448 SNDIPTLEEKELDSMEELVELTLAFGH--DDLFGGFDNDSLPSESKHDVDLDRGGNSGQM 1507
               + ++ E  +   + L E+  A+     DLF        PS       L R G   ++
Sbjct: 1571 -QQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRL-RQGEESKI 1630

Query: 1508 NRFNETEREKNIDL-GGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTLDAV 1567
            N  + + R  +  L  G+++D  L  ++S+     T L                      
Sbjct: 1631 NAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHL---------------------- 1690

Query: 1568 WKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVE---TARLLYSALVLTGVDS 1627
                          + P    ++ E + G   +  +A+G E      LL S        S
Sbjct: 1691 --------------QLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKS 1750

Query: 1628 VETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDT 1687
                     +P ++Y+       IP P    +DP             H  ++ H      
Sbjct: 1751 TMKAIFANLLPGNSYNP------IPFP----FDP-----------DKHYLMYEH---ERV 1810

Query: 1688 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSV-----KMSSIKT 1747
             + V ++EP+SIIA++L   EY   + E  +          LP   ++       S I+ 
Sbjct: 1811 PIAVCEKEPSSIIAFALSCKEYRNALEELSKATLRNSAEEGLPANSALDNRPKSSSPIRL 1870

Query: 1748 CDEEGSDVYRTLRSNEETI-------------------LSMARTESLQFEDLLLF----- 1757
             +  G    RT+ +  +                     LS  + E+L+  D   +     
Sbjct: 1871 PEISGGQTNRTVEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQA 1930

BLAST of Carg25515 vs. TrEMBL
Match: tr|A0A1S4DVR6|A0A1S4DVR6_CUCME (LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like OS=Cucumis melo OX=3656 GN=LOC103487265 PE=4 SV=1)

HSP 1 Score: 2942.5 bits (7627), Expect = 0.0e+00
Identity = 1478/1772 (83.41%), Postives = 1568/1772 (88.49%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPWRSDP NVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFCSKCTANFIP+ STDPTNAREDSE IRVCNYCFKQWGKEPS+L DGDKAT SA+S
Sbjct: 61   CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
            +SSSSTS+GSTKSGYTC SAIS+I STPCS  RQYYDP+CS +IADE D+LRNGKTI R+
Sbjct: 121  VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240
                                 XXXXX        RHI QSD YYC+ N + GLNLEREN 
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240

Query: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300
            STKGSPVNFDTQ PRG+EESSEDSDENDKGSECDFPTY MQNKD+E MDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360
            E                  CAPGEWGHL SS  SGECPMK KSS DY++TAKNVVEGHFR
Sbjct: 301  EXXXXXXXXXXXXXXXXXVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420
            ALVSQLLEAENLP+GD PNEDGWLDI+TYLSWEAAAVLKPDTSKSG MDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKR+ESIIVKGIICKKNVAHRRM + ISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK ITLVLN+KRSLLERIACCTGANI+
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVA LKLGYCD FRV+TFVEEH SGDGQ GKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPL SPI V LPH+PSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNA 720
            S IPGFTCP PVKSPEPE  ME GQSG DAV +RD S+ISHNV+NLNSLEP+L L +SNA
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLLESNA 720

Query: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780
            TSTS SFLKQ+ ST     MLSSK  SE  R+ DS+EYSMG+MTIKEEA+ED D VSSSG
Sbjct: 721  TSTSFSFLKQDFST-----MLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSG 780

Query: 781  KGVPLS-HGNS-IHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQ 840
            K    S H NS I +  +SLD+NTQS NDST Q PE+   +LVTTKSSREDFPLSPSDQQ
Sbjct: 781  KREASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQ 840

Query: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900
            NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH
Sbjct: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900

Query: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960
            VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW
Sbjct: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960

Query: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPS 1020
            GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI+V+SVHIPPS
Sbjct: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020

Query: 1021 IIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQE 1080
            +I+FH NNLEWINKEANEVHNRA+ LF  VCK LPLI K+ESG EQEDFG +ELNNYIQE
Sbjct: 1021 MIEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQE 1080

Query: 1081 LEQIINVEKEDFEVHFNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTL 1140
            LEQIIN+EKE+FEVHFNK  SG TTV QPTVDIFEINKLHMH+LFLSYIWVQRL+Y  TL
Sbjct: 1081 LEQIINMEKENFEVHFNKVLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATL 1140

Query: 1141 GKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDR 1200
             KKHSPD SSNDI TLEEKEL+S+EELVELTLA G D+ FGG DNDSLP ESK +  L R
Sbjct: 1141 RKKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPESKREAGLGR 1200

Query: 1201 GGNSGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLS 1260
             GNSG ++R NET REKNIDLGGQEDD CL  VNSEP+DVG  +RR NS GEYP MTDLS
Sbjct: 1201 EGNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLS 1260

Query: 1261 GTLDAVWKGALHPNSMLFDDRYPQTSSSILEPLAG-PDMEKCTANGVETARLLYSALVLT 1320
            GTLDAVWKG +HPNS++F+D + +T+S+ILEPLAG  DMEKCTANG+ETA LL+SALVLT
Sbjct: 1261 GTLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTANGIETAHLLHSALVLT 1320

Query: 1321 GVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVD 1380
              DSVET T+ TSMP SAY++KISLLNIPK DISDY    I+S+RELEKQ HLRL MHV 
Sbjct: 1321 SDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTC 1440
            SSDTVVPVYDEEPTSIIAYSLLMPEY AQMSEP + MEA DTARSLP IGSVKM+SIKTC
Sbjct: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSVKMNSIKTC 1440

Query: 1441 DEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITC 1500
            DEEGSD Y+TLRSNEETILSM RTESLQFEDL+  TKDLHTRVCF+NETPLGPVKYTITC
Sbjct: 1441 DEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLGPVKYTITC 1500

Query: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
            YFAKRFEALRKKCCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIELE
Sbjct: 1501 YFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHN 1620
            SFIQF+ AYF+YLS++I TGCPTCLAKVVGMYQVS+KHLKGGKE K+DVLVMENLLF HN
Sbjct: 1561 SFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRHN 1620

Query: 1621 VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSF 1680
            VTRLYDLKGSSRSRYN DTSG NKVLLDQNLIESMPTSPIFLG KAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680

Query: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740
            LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTTYFLMINGPPPRSALVSP 1769
            SPHQYKKRFRKAMTTYFLM+    P S+ VSP
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWP-SSTVSP 1766

BLAST of Carg25515 vs. TrEMBL
Match: tr|A0A0A0M3A5|A0A0A0M3A5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G662800 PE=4 SV=1)

HSP 1 Score: 2901.7 bits (7521), Expect = 0.0e+00
Identity = 1459/1772 (82.34%), Postives = 1560/1772 (88.04%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFCS+CTANFIP+PSTD TNAREDSE IRVCNYCFKQWGKEPS+L DGDKAT SA+S
Sbjct: 61   CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKTIKRS 180
            LSSSSTS+GSTKSGYTC SAIS+I STPCS  RQYYDP+CS ++ADE D+LRNGKTI R+
Sbjct: 121  LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181  TSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTEEGLNLERENM 240
                                 XXXXX        RHI QSD YYC+ N +EG NLEREN 
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241  STKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYGMQNKDNEAMDFQNNGILWLPP 300
            STKGSPVNFDTQSPRG+EESSEDSDENDKGSECDFP+Y MQNK++E MDFQNNGILWLPP
Sbjct: 241  STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301  EPEDEEDEKALLFNGDDDACAPGEWGHLPSSQGSGECPMKRKSSGDYRRTAKNVVEGHFR 360
                               CAPGEWG+L SS  SGECPMK KSS DY++TAKNVVEGHFR
Sbjct: 301  XXXXXXXXXXXXXXXXXXVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAAAVLKPDTSKSGCMDPGGYVKVKCVA 420
            ALVSQLLEAENLP+GD P EDGWLDI+TYLSWEAAAVLKPDTSKSG MDPGGYVKVKCVA
Sbjct: 361  ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480
            GGKR+ESIIVKGIICKKNVAHRRM + ISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKKITLVLNVKRSLLERIACCTGANIV 540
            DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK ITLVLN+KRSLLERIACCTGANI+
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVA LKLGYCD FRV+TFVEEH SGDGQ GKKSTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLKSPIIVGLPHSPSNIDRSI 660
            SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPL SPI V LPH+PSN+DRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNVDRSI 660

Query: 661  STIPGFTCPSPVKSPEPESSMETGQSGNDAVPTRDSSMISHNVDNLNSLEPSLRLEKSNA 720
            S IPGFTCP PVKSPEPE  ME GQSG DAV +RDSS+ISHNV NLNSLEP+  L +SNA
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSSLLESNA 720

Query: 721  TSTSLSFLKQNVSTADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSG 780
            TS S SFLKQ+ ST     M+SS   SE  R+  S+EYSMG+M IKEEA EDDD VSSSG
Sbjct: 721  TSPSFSFLKQDFST-----MISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDPVSSSG 780

Query: 781  KGVPLS-HGNS-IHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQ 840
            K    S H N+ + +  +SLDANTQS N+ST Q PE+   +LVTTKSSREDFPLSPSDQQ
Sbjct: 781  KREASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPLSPSDQQ 840

Query: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900
            NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH
Sbjct: 841  NILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAH 900

Query: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960
            VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW
Sbjct: 901  VHCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAW 960

Query: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPS 1020
            GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAI+V+SVHIPPS
Sbjct: 961  GLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS 1020

Query: 1021 IIDFHHNNLEWINKEANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQE 1080
            +I+FH NNLEWINKEANEVHNRA+ LF  VCK LPLI K+ESG EQEDFGV+ELNNYIQE
Sbjct: 1021 MIEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDELNNYIQE 1080

Query: 1081 LEQIINVEKEDFEVHFNK-ASGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTL 1140
            LEQIIN+EKE+FEVHFNK  SG TTV QPTVDIFEINKLHMH+LFLSYIWVQRL+Y  TL
Sbjct: 1081 LEQIINMEKENFEVHFNKLLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATL 1140

Query: 1141 GKKHSPDSSSNDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDR 1200
             KKHSPD SSNDI  L EKEL+S+EELVELTLA G D+ FGG DNDSLP+ESK +  L R
Sbjct: 1141 RKKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPAESKREAGLGR 1200

Query: 1201 GGNSGQMNRFNETEREKNIDLGGQEDDLCLYQVNSEPLDVGTALRRTNSEGEYPSMTDLS 1260
             GNSG ++R N+T+REKNIDLGGQEDD CL  VN +P+DVG  +RR NS GEYP MT + 
Sbjct: 1201 EGNSGNISRLNKTDREKNIDLGGQEDDFCLSPVNLDPVDVGIGIRRANSVGEYPIMT-VP 1260

Query: 1261 GTLDAVWKGALHPNSMLFDDRYPQTSSSILEPLAG-PDMEKCTANGVETARLLYSALVLT 1320
            GTLDAVWKG +HPNS++FDD + +T+S+ILEPLAG  DMEKCTAN +ETA LL+SALVLT
Sbjct: 1261 GTLDAVWKGVIHPNSLIFDDSFSRTTSAILEPLAGQSDMEKCTANRIETAHLLHSALVLT 1320

Query: 1321 GVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVD 1380
              DSVET  + TSMP SAY++KISLLNIPK DISDY    I+S+RELEKQ HLRL MHV 
Sbjct: 1321 RDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHVG 1380

Query: 1381 SSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTC 1440
            SSDTVVPVYDEEPTSIIAYS+LMPEY AQMSEP + MEA DTARSLP IGSVKM+SIKTC
Sbjct: 1381 SSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSLPVIGSVKMNSIKTC 1440

Query: 1441 DEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITC 1500
            DEEGSD Y+TLRSNEETILSM RTESLQFEDL+  +KDLHTRVCFINETPLGPVKYTITC
Sbjct: 1441 DEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFINETPLGPVKYTITC 1500

Query: 1501 YFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560
            YFAKRFEALRKKCCPSELD+VRSLSRC+RWGAQGGKSNVFFAKTLDERFIIKQVTKIELE
Sbjct: 1501 YFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELE 1560

Query: 1561 SFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHN 1620
            SFIQF+ AYF+YLS++I TGCPTCLAKVVGMYQVS+KHLKGGKE K+DVLVMENLLF HN
Sbjct: 1561 SFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRHN 1620

Query: 1621 VTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSF 1680
            VTRLYDLKGSSRSRYN DTSGKNKVLLDQNLIESMPTSPIFLG KAKRLLERAVWNDTSF
Sbjct: 1621 VTRLYDLKGSSRSRYNPDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSF 1680

Query: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740
            LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI
Sbjct: 1681 LASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVI 1740

Query: 1741 SPHQYKKRFRKAMTTYFLMINGPPPRSALVSP 1769
            SPHQYKKRFRKAMTTYFLM+    P S+ VSP
Sbjct: 1741 SPHQYKKRFRKAMTTYFLMVPDQWP-SSTVSP 1765

BLAST of Carg25515 vs. TrEMBL
Match: tr|A0A2C9UB18|A0A2C9UB18_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_16G089800 PE=4 SV=1)

HSP 1 Score: 1873.2 bits (4851), Expect = 0.0e+00
Identity = 1013/1813 (55.87%), Postives = 1254/1813 (69.17%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MG+ +NK SD V +VKSW+P RS+P NVSRDFWMPD+SCRVCY+CDSQFT  NRRHHCR 
Sbjct: 1    MGNSDNKRSDFVDIVKSWIPRRSEPANVSRDFWMPDQSCRVCYECDSQFTIFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFC+KCTA+ IP+PS DP    ED E IRVCNYCFKQW +  +A+ +G       +S
Sbjct: 61   CGRVFCAKCTASSIPAPSEDPRTGSEDWERIRVCNYCFKQWQQGTTAVDNGTNVGSPGLS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSG----------SIADEQDS 180
             S S+TS+ S+KS  TC S+ S + STPCS+      P+ SG              EQ++
Sbjct: 121  PSPSATSLVSSKSCCTCNSS-STVGSTPCSSGAYQSVPYSSGLSPQQSAPMDRTVVEQEN 180

Query: 181  LRNGKTIKRSTSLMTSSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSANTE 240
            L  G++     +   SS++ +GYC S  +D              RH   +D YY     +
Sbjct: 181  LTCGRSSDAPAAAFCSSANQFGYCMSRSDDEDDEYGVYHSGSGTRHFSHADGYYGPGTVD 240

Query: 241  E--------GLNLERE---NMSTKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYG 300
            +        G+  E +   ++S      NFDTQ     +   E++  +D  +EC+ P+YG
Sbjct: 241  DIDHMYEPCGIMHEDQIDRSISCPPLSENFDTQVVDKIKNDGEEAYRHD-CNECESPSYG 300

Query: 301  MQNKDNEAMDFQNNGILWLPPEPEDEEDEK-ALLFNGDD-DACAPGEWGHL--PSSQGSG 360
            +   D E +DF+NNG+LWLPPEPED   E+ A+LF+ DD D    GEWG+L   +S GSG
Sbjct: 301  VNGADAEPVDFENNGLLWLPPEPEDXXXEREAVLFDEDDGDEATTGEWGYLRPSNSFGSG 360

Query: 361  ECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLSWEAA 420
            E   K KSS ++R+  KNVVEGHFRALV+QLL+ ENL VGDE + + WL+I+T LSWEAA
Sbjct: 361  EYRSKDKSSEEHRKAMKNVVEGHFRALVAQLLQVENLSVGDEDDNESWLEIITSLSWEAA 420

Query: 421  AVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKPRFFV 480
             +LKPDTS SG MDPGGYVKVKC+A G+R+ES++VKG++CKKNVAHRRM  +I KPRF +
Sbjct: 421  TLLKPDTSMSGGMDPGGYVKVKCIASGRRSESMVVKGVVCKKNVAHRRMMSKIDKPRFLI 480

Query: 481  LGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSK 540
            LGGALEYQRV+NHLSS DTLLQQE+DHLKMAVAKIDAHHP+VLLVEKSVSR+AQ+YLL+K
Sbjct: 481  LGGALEYQRVSNHLSSVDTLLQQEMDHLKMAVAKIDAHHPSVLLVEKSVSRYAQDYLLAK 540

Query: 541  KITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDGQAGK 600
             I+LVLN+KR LLERIA CTGA+IVP++DH+   KLGYCD F V+ F+EEHGS  GQ GK
Sbjct: 541  DISLVLNIKRPLLERIARCTGAHIVPSIDHLNSQKLGYCDLFHVEKFLEEHGSA-GQGGK 600

Query: 601  KSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLADEGAS 660
            K TKTLMFFEGCPKPL  TILL+GA GD+LKKVKHVVQY  FAAYHLALET FLADEGAS
Sbjct: 601  KLTKTLMFFEGCPKPLGFTILLKGAHGDDLKKVKHVVQYGVFAAYHLALETSFLADEGAS 660

Query: 661  VPELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAV---- 720
            +PELPLKSP+ V LP  P +IDRSISTIPGFT P   K    +   E  +S ND +    
Sbjct: 661  LPELPLKSPLTVALPDKPLSIDRSISTIPGFTVPGTGKLSSCQPPSEVQKSNNDVISDVP 720

Query: 721  -PTRDSSMISHNVDNLNSLEPSLRLEKSNATS---------TSLSFLKQNVSTADRNYML 780
             PT    +      +  S   S ++E  N  S         TSLS L QN S    N + 
Sbjct: 721  SPTNFELVCKSGYADSTSPSNSSQIEYRNLGSNSTEHAGSWTSLSPLGQNTSGPCHNKLF 780

Query: 781  SSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSSGKGVPLSHGNSIHIRSSSLDAN 840
            S  +  + +   ++EE   G     ++A  D  +  +S     L  G        ++ A 
Sbjct: 781  SDHVPGK-DYMTEAEELFQG-----KKANSDSPSYYTS-----LEQGTGSGHADENVLAV 840

Query: 841  TQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLL 900
             Q  N            N     SS+EDFP SPSD Q+ILVSLSTRC+WKGT+C+RAHL 
Sbjct: 841  KQDGN------------NHEEIGSSKEDFPPSPSDHQSILVSLSTRCIWKGTVCERAHLF 900

Query: 901  RIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISAKKLPDFFL 960
            RIKYYGSFDKPLGRFLRD+LFDQNYRC SC MPSEAHV+CYTH QGSLTIS K+LP+F L
Sbjct: 901  RIKYYGSFDKPLGRFLRDHLFDQNYRCRSCEMPSEAHVYCYTHRQGSLTISVKRLPEFLL 960

Query: 961  PGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAG 1020
            PGE EGKIWMWHRCL+CPR+NGFPPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA 
Sbjct: 961  PGEREGKIWMWHRCLRCPRSNGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVAS 1020

Query: 1021 CGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFHHNNLEWINKEANEVHNRA 1080
            CGH LHRDCLRFYGFGR VACF YA+IDV SVH+PPS ++F+++N EWI KEANEV  RA
Sbjct: 1021 CGHSLHRDCLRFYGFGRMVACFRYASIDVHSVHLPPSKLEFNYDNQEWIQKEANEVRQRA 1080

Query: 1081 EVLFAVVCKTLPLILKH--ESGTEQEDFGVNELNNY-IQELEQIINVEKEDFEVH-FNKA 1140
            E+LF  V   L  I +     G++  D   +EL+ + + EL+ I+  EK +FE    N  
Sbjct: 1081 ELLFTEVQNALHRISEKILGEGSQNGDIKSSELSRFHVAELKSILQKEKAEFEESLLNTL 1140

Query: 1141 SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPTLEEKE 1200
            S E  VG P +D+ E+NKL   +LF SY+W QRL YAV+L   +  +S  N IP L  K 
Sbjct: 1141 SKEVKVGHPLIDLLEVNKLRRQILFHSYVWDQRLIYAVSLSNTNHQESMRNFIPKLGGKR 1200

Query: 1201 LDSMEELVELTLAFGHDDLFGGFDN-DSLPSESKHDVDLDRGGNSGQMNRFNETEREKNI 1260
            ++S+E LVE+  +        GF N DS+   +K +++L++GGN G   +        NI
Sbjct: 1201 INSVESLVEMDTS---PKPGKGFSNCDSVIVHTKPELNLNQGGNDGSSRQSVRVHEGGNI 1260

Query: 1261 --DLGGQEDDLCLYQVNS-----EPLDVGTALRRTNSEGEYPSMTDLSGTLDAVWKGALH 1320
              DL  ++ + CL    +     +P + G  +RR +SEGE+P + +LS TLDA W G  H
Sbjct: 1261 RLDLKLKDSEHCLSSCENSNEKIDPSESGNVMRRVHSEGEFPIVDNLSDTLDAAWTGKNH 1320

Query: 1321 PNSMLFDDRYPQ--TSSSILEPLAGPDMEKCTAN--GVETARLLYSALVLTGVDSVETCT 1380
              SM+  +       SS +   +   ++E+C  +  G++    L SAL      +VE  +
Sbjct: 1321 LESMILKENGVSLPDSSPVHSVVPNVELERCIVDKGGIDVVHSLDSALGAKVPQNVENSS 1380

Query: 1381 SLTSMPSSAYSS--KISLLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDTVVP 1440
             L +   + YSS  + S LN  K  I++Y+P  ++ FR+LE+  + RL + V  +DT++P
Sbjct: 1381 GLGTPFPNLYSSFKRTSSLNAQKLGINEYNPVYVSLFRDLERPNNARLLLPVGVNDTIIP 1440

Query: 1441 VYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEGSDV 1500
            V+D+EPTSIIAY+L+  +YH QMSE E+  +A +T  SLP   SV + S  + DE  SD+
Sbjct: 1441 VFDDEPTSIIAYALVSSDYHLQMSELEKPKDAGETTISLPLFDSVNLLSFNSFDESASDI 1500

Query: 1501 YRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAKRFE 1560
            YR++ S EE ILS+  +   Q  D L +TKDLH RV F +++  G VKY +TCY AKRFE
Sbjct: 1501 YRSVGSIEENILSIPGSRGTQILDPLSYTKDLHARVSFTDDSVQGKVKYMVTCYCAKRFE 1560

Query: 1561 ALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQFAP 1620
            ALR+ CCPSELD++RSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+F P
Sbjct: 1561 ALRRICCPSELDFIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFGP 1620

Query: 1621 AYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRLYDL 1680
            AYF YLS+SI  G PTCLAK++G+YQVS+KHLKGGKE K+D+LVMENLLF  N+ RLYDL
Sbjct: 1621 AYFKYLSDSIGNGSPTCLAKILGIYQVSSKHLKGGKETKMDLLVMENLLFKRNIVRLYDL 1680

Query: 1681 KGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASIDVM 1740
            KGSSRSRYNSDTSG NKVLLDQNLIESMPTSPIF+G KAKRLLERAVWNDTSFLASIDVM
Sbjct: 1681 KGSSRSRYNSDTSGSNKVLLDQNLIESMPTSPIFVGNKAKRLLERAVWNDTSFLASIDVM 1740

Query: 1741 DYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKK 1757
            DYSLLVG+D E+ ELV+GIIDFMRQYTWDKHLETWVK SGILGG KN  PTVISP QYKK
Sbjct: 1741 DYSLLVGVDTERHELVIGIIDFMRQYTWDKHLETWVKASGILGGSKNTTPTVISPQQYKK 1784

BLAST of Carg25515 vs. TrEMBL
Match: tr|M5W4R3|M5W4R3_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G191000 PE=4 SV=1)

HSP 1 Score: 1834.3 bits (4750), Expect = 0.0e+00
Identity = 1013/1850 (54.76%), Postives = 1260/1850 (68.11%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MG P+NKLS+LV + KSW+P RS+P NVSRDFWMPD+SCRVCYDCDSQFT  NRRHHCR 
Sbjct: 1    MGTPDNKLSELVDIFKSWIPRRSEPPNVSRDFWMPDQSCRVCYDCDSQFTIFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTNAREDSETIRVCNYCFKQWGKEPSALLDGDKATCSAIS 120
            CGRVFC+KCTAN +P+PS +    RED E IRVCNYCF+QW +  + + +G  A    +S
Sbjct: 61   CGRVFCAKCTANSVPAPSDEQRAGREDWERIRVCNYCFRQWEQGIATVDNGPPARSPGLS 120

Query: 121  LSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSG-----------SIADEQD 180
             S S+TS+ STKS  TC    S I STP S       P+ SG           S+   QD
Sbjct: 121  PSPSATSLASTKSSCTCHXXXSTIGSTPYSTGPYQRVPYTSGLSPNQSSPQIDSVTATQD 180

Query: 181  SLRNGKTIKRSTSLMTSSSSYYGY--CRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCSA 240
            +  + ++I    ++  SS +++G+   RSDDED               H   ++ YY + 
Sbjct: 181  NCTSQRSISSDAAMAESSPNHFGFGMNRSDDED--DDYGVYRLDSEPSHFSHANDYYGAV 240

Query: 241  NTEE--------GLNLERENMSTKGSPVNFDTQSPRGDEESSEDSDENDKGSECDFPTYG 300
            N EE         ++L+ +N S+   P  FDTQ   G +E  E+S E++   EC+   Y 
Sbjct: 241  NIEEFDNVYGPHNVHLDGDNTSSL-LPEGFDTQGVEGSQELREESYEHNNCDECETSPYD 300

Query: 301  MQNKDNEAMDFQNNGILWLP------PEPEDEEDEKALLFNGDDDACAPGEWGHLPSSQ- 360
            +Q+ + E +DF+NNG+LWLP                           A GEWG+L SS  
Sbjct: 301  LQSTNAEPVDFENNGLLWLPXXXXXXXXXXXXXXXXXXXXXXXXXXXATGEWGYLRSSNS 360

Query: 361  -GSGECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTYLS 420
             G+GEC  + KS  ++R   KNVVEGHFRALV+QLL+ E+LP+GDE N++ WLDI+T LS
Sbjct: 361  FGNGECRTREKSIEEHRNAMKNVVEGHFRALVAQLLQVESLPLGDEDNKESWLDIITSLS 420

Query: 421  WEAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEISKP 480
            WEAA +LKPDTSK G MDPGGYVKVKC+A G+R ES +VKG++CKKNVAHRRM  +I KP
Sbjct: 421  WEAATLLKPDTSKGGGMDPGGYVKVKCIACGRRNESTVVKGVVCKKNVAHRRMTSKIEKP 480

Query: 481  RFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQEY 540
            RF +LGGALEYQRV+N LSSFDTLLQQE+DHLKMAVAKID+HHPNVLLVEKSVSR+AQ+Y
Sbjct: 481  RFLILGGALEYQRVSNLLSSFDTLLQQEMDHLKMAVAKIDSHHPNVLLVEKSVSRYAQDY 540

Query: 541  LLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSGDG 600
            LL+K I+LVLN+KR LLERIA CTGA IVP++DH+   KLGYCD F V+ F E HGS  G
Sbjct: 541  LLAKDISLVLNIKRPLLERIARCTGAQIVPSIDHLISPKLGYCDIFHVEKFFEVHGSA-G 600

Query: 601  QAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFLAD 660
            Q GKK TKTLMFFEGCPKPL  TILLRGA+GDELKKVKHVVQY  FAAYHLALET FLAD
Sbjct: 601  QGGKKLTKTLMFFEGCPKPLGVTILLRGANGDELKKVKHVVQYGVFAAYHLALETSFLAD 660

Query: 661  EGASVPELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGNDAV 720
            EGAS+PELPLKS I V LP  PS+IDRSISTIPGF+ P+  K   PE+S E  +S   ++
Sbjct: 661  EGASLPELPLKSVITVALPDKPSSIDRSISTIPGFSVPAAGKPQGPEASSELQKSNKGSI 720

Query: 721  PTRDSSMISHNVDNLNSLEPSLRLEKSNATST-------------------SLSFLKQNV 780
               D   +  N+D + ++E +  +  S A  +                   SLS   +++
Sbjct: 721  SDSD---LCTNIDPILNMEGANSICSSKAACSQAFLGVHSSGSVAPRSPFGSLSHPGEDI 780

Query: 781  STADRNYMLSSKLASEANRNPDSEEYSMGIMTIKEEAKEDDDTVSSS-GKGVPLSH-GNS 840
              + R   L    ASE + +   +E  +       EA  +D  +S+S G    L H G +
Sbjct: 781  RDSFRK-KLPGICASENDIDMGCKESFLAKTDKAGEALFNDRLISNSFGASEALEHGGGN 840

Query: 841  IHIRSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVW 900
             H  +  L AN   +   T  +  S   N    +SS+E+FP SPSD Q+ILVSLSTRCVW
Sbjct: 841  SHADNVDLVANLGEAPGFTSIKNHSDNHN-EEVESSKEEFPPSPSDHQSILVSLSTRCVW 900

Query: 901  KGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLT 960
            KGT+C+R+HL RIKYYG+FDKPLGRFLRD+LFDQ+Y C SCGMPSEAHVHCYTH QGSLT
Sbjct: 901  KGTVCERSHLFRIKYYGNFDKPLGRFLRDHLFDQSYLCRSCGMPSEAHVHCYTHRQGSLT 960

Query: 961  ISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSF 1020
            IS KKLP+  LPGE EGKIWMWHRCL+CPRTNGFPPATRRV+MSDAAWGLSFGKFLELSF
Sbjct: 961  ISVKKLPETLLPGEREGKIWMWHRCLRCPRTNGFPPATRRVVMSDAAWGLSFGKFLELSF 1020

Query: 1021 SNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFHHNNLEWI 1080
            SN+AAA+RVA CGH LHRDCLRFYGFGR VACF YA+I V SV++PPS ++F+++N EWI
Sbjct: 1021 SNHAAANRVATCGHSLHRDCLRFYGFGRMVACFSYASIHVHSVYLPPSKLEFYYDNQEWI 1080

Query: 1081 NKEANEVHNRAEVLFAVVCKTLPLIL--KHESGTEQEDFGVNELNNYIQELEQIINVEKE 1140
             KEA+E+ +RAE+LF  +   L  IL  +  +GT+       E ++ I ELE+++  E+E
Sbjct: 1081 QKEADEMGHRAELLFTELRNALNQILGKRPLAGTQDGGKKAPESSHQIAELEEMLQKERE 1140

Query: 1141 DFEVHFNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSS 1200
            DFE    K    E   G P +DI EINKL   +LF SY+W QRL +A +L  K   +  S
Sbjct: 1141 DFEESLRKVMHREVKFGHPAIDILEINKLRRQLLFHSYVWDQRLIHAASLSNKGFQEGLS 1200

Query: 1201 NDIPTLEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGNSGQMNRF 1260
            + +P L+EK L SME+L E  +        G    DS   E+K D+++++GG+ G  +  
Sbjct: 1201 SSLPKLKEKPLSSMEKLAETNI--NSKPGKGVSICDSSLLETKPDINVNQGGDVGYFSPP 1260

Query: 1261 N--ETEREKNIDLG-GQEDDLCLYQV-----NSEPLDVGTALRRTNSEG-EYPSMTDLSG 1320
               + + E  +DL    E DL           S+PL+ G ++RR  SEG E P++ +LS 
Sbjct: 1261 GGVQNKTEMGLDLNHSNEADLSTPSFPNVIDKSDPLESGKSVRRALSEGDECPTVANLSD 1320

Query: 1321 TLDAVWKGALHPNS--------MLFDDRYPQTSSSILEPLAGPDMEKCTAN--GVETARL 1380
            TLDA W G  HP S         + D     + ++I +  +  D++  T +  GV+    
Sbjct: 1321 TLDAAWTGESHPTSTIPKDNGYSIPDSTLVNSPTAIRKVASNSDLQNYTIDQVGVQVTHS 1380

Query: 1381 LYSALVLTGVDSVETCTSLTSMPSSAYSSKISLLNIPKPDISDYDPALITSFRELEKQGH 1440
            L S L L G D                  K   LN  K  I + +P  +  FRELE+Q  
Sbjct: 1381 LSSPLHLKGFD------------------KNISLNAQKLFIGEGNPVYVPLFRELERQSG 1440

Query: 1441 LRLFMHVDSSDTVVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSV 1500
             RL + +  +DTV+PV+D+EPTSIIAY+L+ P+YH Q+SE ER  +ALD++ SLP   S 
Sbjct: 1441 ARLLLPIGVNDTVIPVFDDEPTSIIAYALVSPDYHLQISESERPKDALDSSVSLPLFDSA 1500

Query: 1501 KMSSIKTCDEEGSDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLG 1560
             + S+ + DE  S+ YR L S++E+++S +R+ S Q  D LL +KDLH RV F ++ PLG
Sbjct: 1501 NLLSLTSFDEAVSETYRNLGSSDESLISTSRSRSSQALDSLL-SKDLHARVSFTDDGPLG 1560

Query: 1561 PVKYTITCYFAKRFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIK 1620
             VKYT+TCY+A RFEALR+ CCPSE+D+VRSLSRCK+WGAQGGKSNVFFAKTLD+RFIIK
Sbjct: 1561 KVKYTVTCYYATRFEALRRTCCPSEIDFVRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIK 1620

Query: 1621 QVTKIELESFIQFAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVM 1680
            QVTK ELESFI+FAP+YF YLSESI T  PTCLAK++G+YQVS+KH KGGKE K+DVLVM
Sbjct: 1621 QVTKTELESFIKFAPSYFKYLSESISTRSPTCLAKILGIYQVSSKHGKGGKESKMDVLVM 1680

Query: 1681 ENLLFGHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLER 1740
            ENLLF  NVTRLYDLKGSSRSRYN DTSG NKVLLDQNLIE+MPTSPIF+G KAKRLLER
Sbjct: 1681 ENLLFRRNVTRLYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLER 1740

Query: 1741 AVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGP 1779
            AVWNDT+FLASIDVMDYSLLVG+DEEK ELV+GIIDF+RQYTWDKHLETWVK SG+LGGP
Sbjct: 1741 AVWNDTAFLASIDVMDYSLLVGVDEEKDELVLGIIDFVRQYTWDKHLETWVKTSGLLGGP 1800

BLAST of Carg25515 vs. TrEMBL
Match: tr|A0A2P5CYF2|A0A2P5CYF2_PARAD (Chaperonin Cpn60/TCP-1 family OS=Parasponia andersonii OX=3476 GN=PanWU01x14_113000 PE=4 SV=1)

HSP 1 Score: 1830.5 bits (4740), Expect = 0.0e+00
Identity = 1000/1816 (55.07%), Postives = 1244/1816 (68.50%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MG P+ KLS++V +V+SW+P RS+P NVSRDFWMPD+SCRVCY+CDSQFT  NRRHHCR 
Sbjct: 1    MGTPDKKLSEIVDMVRSWIPRRSEPTNVSRDFWMPDQSCRVCYECDSQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANFIPSPSTDPTN---AREDSETIRVCNYCFKQWGKEPSALLDGDKATCS 120
            CGRVFC+KCTAN IP+ S +P +    RED E IRVC+YC+KQW +  + + +G  A   
Sbjct: 61   CGRVFCAKCTANSIPALSNEPRSPRTGREDGERIRVCSYCYKQWEQGITTVDNGTGAPSP 120

Query: 121  AISLSSSSTSIGSTKSGYTCQSAISNIPSTPCSNDRQYYDPFCSGSIADEQDSLRNGKT- 180
             +S S S+ S+ STKS YT QS+ S + STP S     + P+ S S++  Q S  +  T 
Sbjct: 121  GLSPSPSAVSLASTKSSYTGQSSSSTVGSTPYSTGPYQHVPYGS-SLSPRQSSQMDSVTG 180

Query: 181  ------IKRSTSLMT----SSSSYYGYCRSDDEDGXXXXXXXXXXXXXRHIPQSDHYYCS 240
                   +RST+L T    S  + Y +C +  +D              RH    D YY +
Sbjct: 181  QEGNSASQRSTNLNTVMEDSFPNQYSFCSNRSDDEVDDYGAYHSESVTRHFSPVDGYYGA 240

Query: 241  ANTEE--------GLNLERENMSTKG-----SPVNFDTQSPRGDEESSEDSDENDKGSEC 300
             + +E         L+ E +N+  K       P  +D Q     E   E++DE++   E 
Sbjct: 241  VSIDEIDQVYGPHDLHPEEDNIDVKNLGYSPLPETYDAQGMVETERVGEEADEHENSDEH 300

Query: 301  DFPTYGMQNKDNEAMDFQNNGILWLPPEPEDEEDEKALLFNGDDDAC--APGEWGHLPSS 360
            + P+Y    ++ E +DF+N+G+LWLPP                        GEWG+L SS
Sbjct: 301  EAPSYDEDGRNVEPVDFENSGLLWLPPXXXXXXXXXXXXXXXXXXXXXGVTGEWGYLRSS 360

Query: 361  Q--GSGECPMKRKSSGDYRRTAKNVVEGHFRALVSQLLEAENLPVGDEPNEDGWLDILTY 420
               GSGE   + KSS ++R   KNVVEGHFRALV+QLL+ ENLPVGD+ +++ WLD++T 
Sbjct: 361  NSFGSGEYRSREKSSEEHRNAMKNVVEGHFRALVAQLLQVENLPVGDDNDKESWLDVITS 420

Query: 421  LSWEAAAVLKPDTSKSGCMDPGGYVKVKCVAGGKRTESIIVKGIICKKNVAHRRMRKEIS 480
            LSWEAA +LKPD SK G MDPGGYVKVKC+A G R+ES ++KG++CKKNVAHRRM   I 
Sbjct: 421  LSWEAATLLKPDMSKGGGMDPGGYVKVKCIACGSRSESTVIKGVVCKKNVAHRRMTSRID 480

Query: 481  KPRFFVLGGALEYQRVTNHLSSFDTLLQQELDHLKMAVAKIDAHHPNVLLVEKSVSRHAQ 540
            KPRF +LGGALEYQRV+N LSSFDTLLQQE+DHLKMAVAKIDAHHP+VLLVEKSVSR+AQ
Sbjct: 481  KPRFLILGGALEYQRVSNLLSSFDTLLQQEMDHLKMAVAKIDAHHPDVLLVEKSVSRYAQ 540

Query: 541  EYLLSKKITLVLNVKRSLLERIACCTGANIVPTVDHVACLKLGYCDEFRVKTFVEEHGSG 600
            +YLL+K I+LVLN+K+ LL+RI+ CTGA IV ++DH+A  KLGYCD F V+ F+EEHGS 
Sbjct: 541  DYLLAKNISLVLNIKKPLLDRISRCTGAQIVSSIDHLASPKLGYCDMFHVEKFLEEHGSA 600

Query: 601  DGQAGKKSTKTLMFFEGCPKPLCCTILLRGASGDELKKVKHVVQYATFAAYHLALETCFL 660
             GQ GKK  KTLMFFEGCPKPL CTILL+GASGDELKKVKHVVQY  FAAYHLALET FL
Sbjct: 601  -GQGGKKLIKTLMFFEGCPKPLGCTILLKGASGDELKKVKHVVQYGVFAAYHLALETSFL 660

Query: 661  ADEGASVPELPLKSPIIVGLPHSPSNIDRSISTIPGFTCPSPVKSPEPESSMETGQSGND 720
            ADEGAS+PE PL+SPI V LP  PS IDRSIST+     P+  K   PE+  ET +S N 
Sbjct: 661  ADEGASIPEFPLRSPITVALPDKPSGIDRSISTV---GVPATGKPLGPEAVNETEKSNNG 720

Query: 721  AVPTRDSSMISHNVDNLNSLEPSLRL-----EKSNATSTSLSFLKQNVSTADRNYMLSSK 780
             V  RD S   + +  L   E    L       S A++TSLS L Q+ S    N++    
Sbjct: 721  VVLDRDLSTNHNPIFKLEVDESPFHLAPRYSPHSVASTTSLSPLGQDSSAWSHNHLFPID 780

Query: 781  LASEANR-NPDSEEYSMGIMTIKEEAKEDDDTVSSS---------GKGVPLSHGN-SIHI 840
            ++ +AN  +P+ +  +    T   ++  D   VS+S         G G   + GN S+  
Sbjct: 781  VSKDANSLDPEFQAKT----TNNNKSLVDYSVVSTSFGTSETFEEGNGTRRADGNTSVDR 840

Query: 841  RSSSLDANTQSSNDSTCQRPESLIANLVTTKSSREDFPLSPSDQQNILVSLSTRCVWKGT 900
             +  + +  QS ND    R E +        SS+E+FP SPSD Q+ILVSLSTRCVWKGT
Sbjct: 841  GTLKIASIRQSGND----RGEEV-------GSSKEEFPPSPSDHQSILVSLSTRCVWKGT 900

Query: 901  ICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHVHCYTHPQGSLTISA 960
            +C+RAHL RIKYYG+ DKPLGRFLRD+LFDQ+Y C SCGMPSEAHVHCYTH QGSLTIS 
Sbjct: 901  VCERAHLFRIKYYGNCDKPLGRFLRDHLFDQSYHCRSCGMPSEAHVHCYTHRQGSLTISV 960

Query: 961  KKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNN 1020
            KKLP+F LPGE EGKIWMWHRCL+CPRTNGFPPATRRV+MSDAAWGLSFGKFLELSFSN+
Sbjct: 961  KKLPEFLLPGEKEGKIWMWHRCLRCPRTNGFPPATRRVVMSDAAWGLSFGKFLELSFSNH 1020

Query: 1021 AAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAIDVKSVHIPPSIIDFHHNNLEWINKE 1080
            AAASRVA CGH LHRDCLRFYGFGR VACF YA+IDV SV++PP  ++F++++ EWI KE
Sbjct: 1021 AAASRVASCGHSLHRDCLRFYGFGRMVACFRYASIDVHSVYLPPPKLEFYYDDREWIQKE 1080

Query: 1081 ANEVHNRAEVLFAVVCKTLPLILKHESGTEQEDFGVNELNNYIQELEQIINVEKEDFEVH 1140
            A+EV N+  +LF  V   L  + +  S     +  + E    I EL  ++  EKE+F+  
Sbjct: 1081 ADEVRNQVGLLFTEVQNALYQVSQKISTMGLPEAVLKESRQRIAELGGMLQKEKEEFQES 1140

Query: 1141 FNKA-SGETTVGQPTVDIFEINKLHMHVLFLSYIWVQRLSYAVTLGKKHSPDSSSNDIPT 1200
              KA   E  VGQP +DI EINKL   +LF SY W QRL +A +L K    +S S+ +P 
Sbjct: 1141 VQKALCSELKVGQPAIDILEINKLRRQILFSSYAWDQRLIHAASLTKNSHLESLSSSVPK 1200

Query: 1201 LEEKELDSMEELVELTLAFGHDDLFGGFDNDSLPSESKHDVDLDRGGNSGQM-NRFNETE 1260
            L+EK + S+E++ E+ +      + G    DS  SE+K + +L+   NS Q+  + +E +
Sbjct: 1201 LKEKSVSSLEKINEMNVT--SKPVKGFSSCDSYLSETKPETNLNERENSEQICQKDSEAQ 1260

Query: 1261 REKNIDLG---GQEDDLCL-----YQVNSEPLDVGTALRRTNSEGEYPSMTDLSGTLDAV 1320
                I LG     ED+ CL         S+PL+    +RRT+S+GEYP   DLS TLDA 
Sbjct: 1261 NGNKIGLGQSYSSEDEACLSSGADISDKSQPLESAKLVRRTHSDGEYPVGADLSDTLDAA 1320

Query: 1321 WKGALHPNSMLFDDRYPQTSSSILEPLAGPDMEKCTANGVETARLLYSALVLTGVDSVET 1380
            W G    +    DD    + S+++      D  K     VE  R + SAL     D++E 
Sbjct: 1321 WTGEYPTSVTSKDDGSFSSDSTVVNSTF--DQGK-----VEAIRSVGSALSFKEHDNLEN 1380

Query: 1381 CTSLTSMPSSAYSSKIS---LLNIPKPDISDYDPALITSFRELEKQGHLRLFMHVDSSDT 1440
             TSLTS+P   +SS ++    L   K    +Y+P  +  FRELE++   RL + V  +DT
Sbjct: 1381 STSLTSVPFMNFSSSVNKNLSLGAQKLCSGEYNPVYVLLFRELERKSGARLLLPVGDNDT 1440

Query: 1441 VVPVYDEEPTSIIAYSLLMPEYHAQMSEPERVMEALDTARSLPTIGSVKMSSIKTCDEEG 1500
            V+PVYD+EPTS+IAY+L+  EYH Q+SE E+  +A+DT+ SLP   S+   S+ + DE  
Sbjct: 1441 VIPVYDDEPTSVIAYTLVSKEYHLQLSESEKRKDAVDTSVSLPLFDSLNFLSLNSFDESV 1500

Query: 1501 SDVYRTLRSNEETILSMARTESLQFEDLLLFTKDLHTRVCFINETPLGPVKYTITCYFAK 1560
            +D YR+  S++E+ILSM+   S Q  D  L++KDLH R+ F ++ PLG VKYT+ CY+AK
Sbjct: 1501 ADSYRSFGSSDESILSMSALRSFQSVDPFLYSKDLHIRISFTDDGPLGKVKYTVACYYAK 1560

Query: 1561 RFEALRKKCCPSELDYVRSLSRCKRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFIQ 1620
             FEALR+ CCPSELD+VRSLSRCK+WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESFI+
Sbjct: 1561 SFEALRRICCPSELDFVRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIK 1620

Query: 1621 FAPAYFLYLSESIRTGCPTCLAKVVGMYQVSTKHLKGGKEFKIDVLVMENLLFGHNVTRL 1680
            F PAYF YLSESI TGCPTCLAK++G+YQVS+KH KGGKE ++DVLVMENLLF  +VTRL
Sbjct: 1621 FGPAYFKYLSESISTGCPTCLAKILGIYQVSSKHFKGGKEARMDVLVMENLLFRRHVTRL 1680

Query: 1681 YDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGGKAKRLLERAVWNDTSFLASI 1740
            YDLKGSSRSRYN DTSG NKVLLDQNLIE+MPTSPIF+G KAKRLLERAVWNDTSFLASI
Sbjct: 1681 YDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTSFLASI 1740

Query: 1741 DVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQ 1757
             VMDYSLLVG+DEEK ELV+GIIDFMRQYTWDKHLETWVK SG LGGPKN +PTVISP Q
Sbjct: 1741 GVMDYSLLVGVDEEKHELVLGIIDFMRQYTWDKHLETWVKTSGFLGGPKNTSPTVISPEQ 1787