Carg25467 (gene) Silver-seed gourd

NameCarg25467
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionWAT1-related protein
LocationCucurbita_argyrosperma_scaffold_256 : 179964 .. 181497 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAACATACTACTTAAGGAGATTCTTAATGAAGGCATCAGTCAGCTACTCATCGTCGTGTATCGACAGACGATTTCCTCTCTTTTTTTGTTGCCAATAGCCTATTTTTGGGAAAGGTAAAGGCACCTTGGTTCATACTAGACACTGCCTTTTCCTTCTTACTTGTCCAAGTTCTTCTTTGTTATGTGAAAAGCTTTTAAATGATTCAAAGCTATAGAGTTCTCTGTTTTTGGTGAAATGCAGGAAAACCAGACCTCAGCTTACACCTTACATCTTATTCCTCCTTTTCATTAGTTCCTTACTTGGGTTAGTATAGACATCCTTTTGTAAGCTTCACCATAAATTTATGATGTTTTTCTCAGTAAAGATACACTAATGCAGGCTAACACTAACATTATACCTATTCCTAATTGGTCTGCACTACACATCTGCAACTTTCTCTTGTGCATTTCTCAATCTGGTGCCCGTAAATACGTTCGTTCTAGCCGTATTACTCCGGTGCGTACCGAAACGAATCATAGTTCATTTTCTTTTCCAATGTGATGATAGTAGTAACTTTGGTTTCAAAATCTTTCTAGAATGGAAAAAGTGGACACGAAAAGCAAGGGTGGAAGAGCCAAAGTGCTTGGGACTCTGGTGTGCATTTCAGGAAACTTACTTCTGATTCTGTACAAAGGAATGCCGTTGACCAGCCCGGGTTCAGCAACTGCGCAAACGAACGGAGCTCGTGTAACAGTTCAAGCTGACACACCAGGAAAGTGGCTAATAGGTTCATTGGTTTTGACTGCAGGGTGCTTTATGTGGTCCTCTTGGTTCCTTATGCAATCAAGAGTTGGCAAGGTTTATCCATGTCAATATTCTAGTACCTGTATCATGTCCTTCTTCAGTGCCATTCAATCAGCTGTTTTACACTTAATTATAGACAGGAAGAACTCCGTGTTCATCGTGAAAGGGACGTTCGCTATCTTAAGCCTCGTATATGCTGTAAGTATCAGTCTTTTTTCCCTCATAACCTATTTGTTGCTAAATTTGTTTGATATTTAATGATGTTGTAATGGGCTTAACAGGGATCAGTGGGTTCAGGGCTGTGCTATGTGGGGATGTCTTGGTGTGTGAAACAGAAAGGGCCAGTTTTTACAGCTGCTTTTACTCCTTTCATGGAGGTTTTTGCAGCCATATTTGATTTCTTCATCTTGCATGAACAGATTCATCTAGGAAGGTCTGCTTTTGTTCTTCATTTTTCTTCCTATTTCTTGATCAAAACTGAGAAATTGTTTCTAAGCTGGGGTTTCTTAAGATCAAAACTAAAAGTTCTGTAGTGTGTGTGGCTTGATTAGGACCAAAAAAATGCTGAAAAATGTGATGCTGTGGCAGTGTTATAGGGTCAGTGATGATCATAAGTGGAATGTATATCCTTCTGTGGGGAAAGGACAGAGAAGCAAAGGAGTGTGCAGTGAACCAAAGGGTATCAGTGGAAGAAGGCTATCGTAAATCAAACATACCAACCACAAGCACAGATGCAGTGGAGAAGATA

mRNA sequence

GTGAACATACTACTTAAGGAGATTCTTAATGAAGGCATCAGTCAGCTACTCATCGTCGTGTATCGACAGACGATTTCCTCTCTTTTTTTGTTGCCAATAGCCTATTTTTGGGAAAGGAAAACCAGACCTCAGCTTACACCTTACATCTTATTCCTCCTTTTCATTAGTTCCTTACTTGGGCTAACACTAACATTATACCTATTCCTAATTGGTCTGCACTACACATCTGCAACTTTCTCTTGTGCATTTCTCAATCTGGTGCCCGTAAATACGTTCGTTCTAGCCGTATTACTCCGAATGGAAAAAGTGGACACGAAAAGCAAGGGTGGAAGAGCCAAAGTGCTTGGGACTCTGGTGTGCATTTCAGGAAACTTACTTCTGATTCTGTACAAAGGAATGCCGTTGACCAGCCCGGGTTCAGCAACTGCGCAAACGAACGGAGCTCGTGTAACAGTTCAAGCTGACACACCAGGAAAGTGGCTAATAGGTTCATTGGTTTTGACTGCAGGGTGCTTTATGTGGTCCTCTTGGTTCCTTATGCAATCAAGAGTTGGCAAGGTTTATCCATGTCAATATTCTAGTACCTGTATCATGTCCTTCTTCAGTGCCATTCAATCAGCTGTTTTACACTTAATTATAGACAGGAAGAACTCCGTGTTCATCGTGAAAGGGACGTTCGCTATCTTAAGCCTCGTATATGCTGGATCAGTGGGTTCAGGGCTGTGCTATGTGGGGATGTCTTGGTGTGTGAAACAGAAAGGGCCAGTTTTTACAGCTGCTTTTACTCCTTTCATGGAGGTTTTTGCAGCCATATTTGATTTCTTCATCTTGCATGAACAGATTCATCTAGGAAGTGTTATAGGGTCAGTGATGATCATAAGTGGAATGTATATCCTTCTGTGGGGAAAGGACAGAGAAGCAAAGGAGTGTGCAGTGAACCAAAGGGTATCAGTGGAAGAAGGCTATCGTAAATCAAACATACCAACCACAAGCACAGATGCAGTGGAGAAGATA

Coding sequence (CDS)

GTGAACATACTACTTAAGGAGATTCTTAATGAAGGCATCAGTCAGCTACTCATCGTCGTGTATCGACAGACGATTTCCTCTCTTTTTTTGTTGCCAATAGCCTATTTTTGGGAAAGGAAAACCAGACCTCAGCTTACACCTTACATCTTATTCCTCCTTTTCATTAGTTCCTTACTTGGGCTAACACTAACATTATACCTATTCCTAATTGGTCTGCACTACACATCTGCAACTTTCTCTTGTGCATTTCTCAATCTGGTGCCCGTAAATACGTTCGTTCTAGCCGTATTACTCCGAATGGAAAAAGTGGACACGAAAAGCAAGGGTGGAAGAGCCAAAGTGCTTGGGACTCTGGTGTGCATTTCAGGAAACTTACTTCTGATTCTGTACAAAGGAATGCCGTTGACCAGCCCGGGTTCAGCAACTGCGCAAACGAACGGAGCTCGTGTAACAGTTCAAGCTGACACACCAGGAAAGTGGCTAATAGGTTCATTGGTTTTGACTGCAGGGTGCTTTATGTGGTCCTCTTGGTTCCTTATGCAATCAAGAGTTGGCAAGGTTTATCCATGTCAATATTCTAGTACCTGTATCATGTCCTTCTTCAGTGCCATTCAATCAGCTGTTTTACACTTAATTATAGACAGGAAGAACTCCGTGTTCATCGTGAAAGGGACGTTCGCTATCTTAAGCCTCGTATATGCTGGATCAGTGGGTTCAGGGCTGTGCTATGTGGGGATGTCTTGGTGTGTGAAACAGAAAGGGCCAGTTTTTACAGCTGCTTTTACTCCTTTCATGGAGGTTTTTGCAGCCATATTTGATTTCTTCATCTTGCATGAACAGATTCATCTAGGAAGTGTTATAGGGTCAGTGATGATCATAAGTGGAATGTATATCCTTCTGTGGGGAAAGGACAGAGAAGCAAAGGAGTGTGCAGTGAACCAAAGGGTATCAGTGGAAGAAGGCTATCGTAAATCAAACATACCAACCACAAGCACAGATGCAGTGGAGAAGATA

Protein sequence

VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLGLTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILLWGKDREAKECAVNQRVSVEEGYRKSNIPTTSTDAVEKI
BLAST of Carg25467 vs. NCBI nr
Match: XP_022927181.1 (WAT1-related protein At3g30340-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 636.7 bits (1641), Expect = 4.4e-179
Identity = 332/338 (98.22%), Postives = 334/338 (98.82%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT YILFLLFISSLLG
Sbjct: 6   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFISSLLG 65

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD KSKGGRAKVLGTLVC
Sbjct: 66  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDMKSKGGRAKVLGTLVC 125

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           ISGNL+LILYKGM LTSPGSATA+TNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM
Sbjct: 126 ISGNLILILYKGMSLTSPGSATAKTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 185

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG
Sbjct: 186 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 245

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL
Sbjct: 246 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 305

Query: 301 WGKDREAKECAVNQRVSVEEGYRKSNIPTTSTDAVEKI 339
           WGKDREAKECAVNQRVSVEEGYR SNIPTTSTDAVEKI
Sbjct: 306 WGKDREAKECAVNQRVSVEEGYRNSNIPTTSTDAVEKI 343

BLAST of Carg25467 vs. NCBI nr
Match: XP_022927182.1 (WAT1-related protein At3g30340-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 636.7 bits (1641), Expect = 4.4e-179
Identity = 332/338 (98.22%), Postives = 334/338 (98.82%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT YILFLLFISSLLG
Sbjct: 3   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFISSLLG 62

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD KSKGGRAKVLGTLVC
Sbjct: 63  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDMKSKGGRAKVLGTLVC 122

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           ISGNL+LILYKGM LTSPGSATA+TNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM
Sbjct: 123 ISGNLILILYKGMSLTSPGSATAKTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 182

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG
Sbjct: 183 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 242

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL
Sbjct: 243 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 302

Query: 301 WGKDREAKECAVNQRVSVEEGYRKSNIPTTSTDAVEKI 339
           WGKDREAKECAVNQRVSVEEGYR SNIPTTSTDAVEKI
Sbjct: 303 WGKDREAKECAVNQRVSVEEGYRNSNIPTTSTDAVEKI 340

BLAST of Carg25467 vs. NCBI nr
Match: XP_022927179.1 (WAT1-related protein At3g30340-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 636.7 bits (1641), Expect = 4.4e-179
Identity = 332/338 (98.22%), Postives = 334/338 (98.82%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT YILFLLFISSLLG
Sbjct: 17  VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFISSLLG 76

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD KSKGGRAKVLGTLVC
Sbjct: 77  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDMKSKGGRAKVLGTLVC 136

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           ISGNL+LILYKGM LTSPGSATA+TNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM
Sbjct: 137 ISGNLILILYKGMSLTSPGSATAKTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 196

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG
Sbjct: 197 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 256

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL
Sbjct: 257 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 316

Query: 301 WGKDREAKECAVNQRVSVEEGYRKSNIPTTSTDAVEKI 339
           WGKDREAKECAVNQRVSVEEGYR SNIPTTSTDAVEKI
Sbjct: 317 WGKDREAKECAVNQRVSVEEGYRNSNIPTTSTDAVEKI 354

BLAST of Carg25467 vs. NCBI nr
Match: XP_023520075.1 (WAT1-related protein At3g30340-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.9 bits (1626), Expect = 2.4e-177
Identity = 329/338 (97.34%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT YILFLLFISSLLG
Sbjct: 3   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFISSLLG 62

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLTLYLFLIGLHYTSATFSCAFLNLV VNTFVLAVLLRMEKVD KSKGGRAKVLGTLVC
Sbjct: 63  LTLTLYLFLIGLHYTSATFSCAFLNLVHVNTFVLAVLLRMEKVDMKSKGGRAKVLGTLVC 122

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           ISGNLLLILYKGMPLTSPGSATA+TNGARVTVQA+TPGKWLIGSLVLTAGCFMWSSWFLM
Sbjct: 123 ISGNLLLILYKGMPLTSPGSATAKTNGARVTVQAETPGKWLIGSLVLTAGCFMWSSWFLM 182

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG
Sbjct: 183 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 242

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSV+GSVMIISGMYILL
Sbjct: 243 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVLGSVMIISGMYILL 302

Query: 301 WGKDREAKECAVNQRVSVEEGYRKSNIPTTSTDAVEKI 339
           WGKDREAKECAV QRVSVEEGYR SNIPTTSTDAVE+I
Sbjct: 303 WGKDREAKECAVKQRVSVEEGYRNSNIPTTSTDAVEQI 340

BLAST of Carg25467 vs. NCBI nr
Match: XP_023520074.1 (WAT1-related protein At3g30340-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.9 bits (1626), Expect = 2.4e-177
Identity = 329/338 (97.34%), Postives = 333/338 (98.52%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT YILFLLFISSLLG
Sbjct: 6   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFISSLLG 65

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLTLYLFLIGLHYTSATFSCAFLNLV VNTFVLAVLLRMEKVD KSKGGRAKVLGTLVC
Sbjct: 66  LTLTLYLFLIGLHYTSATFSCAFLNLVHVNTFVLAVLLRMEKVDMKSKGGRAKVLGTLVC 125

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           ISGNLLLILYKGMPLTSPGSATA+TNGARVTVQA+TPGKWLIGSLVLTAGCFMWSSWFLM
Sbjct: 126 ISGNLLLILYKGMPLTSPGSATAKTNGARVTVQAETPGKWLIGSLVLTAGCFMWSSWFLM 185

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG
Sbjct: 186 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 245

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSV+GSVMIISGMYILL
Sbjct: 246 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVLGSVMIISGMYILL 305

Query: 301 WGKDREAKECAVNQRVSVEEGYRKSNIPTTSTDAVEKI 339
           WGKDREAKECAV QRVSVEEGYR SNIPTTSTDAVE+I
Sbjct: 306 WGKDREAKECAVKQRVSVEEGYRNSNIPTTSTDAVEQI 343

BLAST of Carg25467 vs. TAIR10
Match: AT3G30340.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-86
Identity = 162/314 (51.59%), Postives = 227/314 (72.29%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN++ K++++EG+++++   YR  + +LFL+P A F ER  RP+LT  IL  LF S+LLG
Sbjct: 25  VNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLTGRILCSLFFSALLG 84

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
            +L  Y FLIGL YTS+TFS AF N+VP  TF LA++ R E ++ KS  GRAK+LGT++C
Sbjct: 85  TSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIKSNVGRAKLLGTMIC 144

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           I G L+L LYKG  L+   S   +T+    +  A T  KW +GS++L     +WSSWF++
Sbjct: 145 ICGALVLTLYKGTALSREHSTHMETHTRTDSTGAMTQ-KWAMGSIMLVISIIIWSSWFIV 204

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+++ +VYPCQY+ST I+SFF  IQSA+L LI +R  S+++VK  F +L+L+Y+G VGSG
Sbjct: 205 QAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVKDKFQVLALLYSGIVGSG 264

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWC++Q+G VFT++F P ++VFAAIF F  LHEQI+ GSVIGS++II G+YILL
Sbjct: 265 LCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYCGSVIGSMVIIVGLYILL 324

Query: 301 WGKDREAKECAVNQ 315
           WGK ++       Q
Sbjct: 325 WGKSKDKSASVTKQ 337

BLAST of Carg25467 vs. TAIR10
Match: AT4G01440.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 295.8 bits (756), Expect = 3.3e-80
Identity = 154/308 (50.00%), Postives = 215/308 (69.81%), Query Frame = 0

Query: 2   NILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLGL 61
           N L+K++L+ G++ ++I  YR  IS+LFL PIA+FWERKTRP LT  IL  LF S+L+G 
Sbjct: 25  NALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWERKTRPTLTLNILVQLFFSALVGA 84

Query: 62  TLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVCI 121
           +LT Y FL+GL YTSAT +CAF+++ P  TFV+A++ R+EK++ KSK G   V+G L+CI
Sbjct: 85  SLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKLNMKSKAGMGMVMGALICI 144

Query: 122 SGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLMQ 181
            G LLL +YKG+PLT       +T+       A  P  W+IG ++L AG   + SW L+Q
Sbjct: 145 GGALLLTMYKGVPLTK--LRKLETHQLINNNHAMKPENWIIGCVLLFAGSSCFGSWMLIQ 204

Query: 182 SRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSGL 241
           ++V + YPCQYSST ++SFF  IQ A+L LI  R  + +I+     I+++VYAG+V  G+
Sbjct: 205 AKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILTDKLDIVTIVYAGAVAQGI 264

Query: 242 CYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILLW 301
           C VG SWC++++GP+FT+ FTP   +FA +FDF ILH QI LGSV+GS ++I G+YI L 
Sbjct: 265 CTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFLGSVVGSGVVIFGLYIFLL 324

Query: 302 GKDREAKE 310
           GK R  KE
Sbjct: 325 GKVRLMKE 330

BLAST of Carg25467 vs. TAIR10
Match: AT4G01450.2 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 270.0 bits (689), Expect = 2.0e-72
Identity = 142/312 (45.51%), Postives = 207/312 (66.35%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN L+K++L+ GI+ ++I  YR  IS+LFLLP+AYFWERKTRP+LT  I   LF+S+L G
Sbjct: 24  VNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWERKTRPKLTLSISCQLFVSALFG 83

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
            +L  Y +L+GL YTSAT   AF  ++P  TFV+A++   EK+  K+K G   VLGTL+ 
Sbjct: 84  ASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKLSLKTKIGYGVVLGTLIS 143

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           + G LLL +Y+G+PLT+     A +N             W+ G   L  G  ++SSW L+
Sbjct: 144 LVGGLLLTMYQGIPLTNSPEQAANSN------NHTGHENWIKGCFFLLTGVVLFSSWMLI 203

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+++   YPC YSST I+S F  +Q A+L LI  R    +I++    I+++V AG V  G
Sbjct: 204 QAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWILRDELTIITVVIAGVVAQG 263

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           +C VGMSWC+KQ+GPV +++F+P + + A +FDF ILH +I+LGSVIGSV+++ G+YI L
Sbjct: 264 MCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIYLGSVIGSVVVVIGLYIFL 323

Query: 301 WGKDREAKECAV 313
           W + ++  EC +
Sbjct: 324 WSRSKQIVECKI 329

BLAST of Carg25467 vs. TAIR10
Match: AT4G01430.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 242.3 bits (617), Expect = 4.4e-64
Identity = 139/328 (42.38%), Postives = 195/328 (59.45%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN L+K+ L+ G++ ++   YR  IS+L L+P +Y WERKTRPQLT  +L   FIS LLG
Sbjct: 23  VNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWERKTRPQLTFMLLCEHFISGLLG 82

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKV-DTKSKGGRAKVLGTLV 120
            +L  + FL+GL YTSAT S A ++++P  TF LA++ R+E   + KSK G  KV+GTL+
Sbjct: 83  ASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIFRIENAQNLKSKAGVLKVMGTLI 142

Query: 121 CISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFL 180
           CI G +LL  YKG  L++P S              D   KWL+G L L  G  + S W L
Sbjct: 143 CIMGAMLLTFYKGPELSNPHSHPQARXXXXXXXXXDQTKKWLLGCLYLVIGTVLLSLWML 202

Query: 181 MQSRVGKVYP-CQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVG 240
            Q ++   YP  +YSSTC+MS F++ Q A+L L   R    +I++  F IL  +YAG VG
Sbjct: 203 FQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRDVKDWIIEDKFVILVTLYAGIVG 262

Query: 241 SGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYI 300
             +  V  SW +K  G VF + F+P   V A +FDF ILH  ++LGS++GSV+ I+G+Y+
Sbjct: 263 QAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLILHSPLYLGSILGSVVTITGLYV 322

Query: 301 LLWGKDREAKECAVNQRVSVEEGYRKSN 327
            LWG+  E  +       S +    K N
Sbjct: 323 FLWGRKNETDQSVSKTLNSSQFSQNKDN 350

BLAST of Carg25467 vs. TAIR10
Match: AT1G01070.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 241.5 bits (615), Expect = 7.5e-64
Identity = 142/337 (42.14%), Postives = 199/337 (59.05%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN L+K+ L+ G++ ++I  YR  IS+L L+P AY  ERKTRPQ+T  ++   F+S LLG
Sbjct: 30  VNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRPQITFRLMVDHFVSGLLG 89

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD-TKSKGGRAKVLGTLV 120
            +L  + FL+GL YTSAT SCA ++++P  TF LA++ R E V   K+K G  KV+GTL+
Sbjct: 90  ASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENVKILKTKAGMLKVIGTLI 149

Query: 121 CISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFL 180
           CISG L L  YKG P  S                      WL+G L LT G  + S W L
Sbjct: 150 CISGALFLTFYKG-PQISNXXXXXXXXXXXXXXXXXXANNWLLGCLYLTIGTVLLSLWML 209

Query: 181 MQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGS 240
            Q  +   YPC+YSSTC+MS F+A Q A+L L   R  + +I+   F I  ++YAG VG 
Sbjct: 210 FQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWIIDDRFVITVIIYAGVVGQ 269

Query: 241 GLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYIL 300
            +  V  +W +K+ G VF +AF P   + A +FDF ILH  ++LGSVIGS++ I+G+Y+ 
Sbjct: 270 AMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLYLGSVIGSLVTITGLYMF 329

Query: 301 LWGKDREAK-ECAVNQRVSVEEGYRKSNIPTTSTDAV 336
           LWGK++E +   A++  +  E  Y   N    S   V
Sbjct: 330 LWGKNKETESSTALSSGMDNEAQYTTPNKDNDSKSPV 365

BLAST of Carg25467 vs. Swiss-Prot
Match: sp|Q9LI65|WTR24_ARATH (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-85
Identity = 162/314 (51.59%), Postives = 227/314 (72.29%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN++ K++++EG+++++   YR  + +LFL+P A F ER  RP+LT  IL  LF S+LLG
Sbjct: 25  VNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLTGRILCSLFFSALLG 84

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
            +L  Y FLIGL YTS+TFS AF N+VP  TF LA++ R E ++ KS  GRAK+LGT++C
Sbjct: 85  TSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIKSNVGRAKLLGTMIC 144

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           I G L+L LYKG  L+   S   +T+    +  A T  KW +GS++L     +WSSWF++
Sbjct: 145 ICGALVLTLYKGTALSREHSTHMETHTRTDSTGAMTQ-KWAMGSIMLVISIIIWSSWFIV 204

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+++ +VYPCQY+ST I+SFF  IQSA+L LI +R  S+++VK  F +L+L+Y+G VGSG
Sbjct: 205 QAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVKDKFQVLALLYSGIVGSG 264

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWC++Q+G VFT++F P ++VFAAIF F  LHEQI+ GSVIGS++II G+YILL
Sbjct: 265 LCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYCGSVIGSMVIIVGLYILL 324

Query: 301 WGKDREAKECAVNQ 315
           WGK ++       Q
Sbjct: 325 WGKSKDKSASVTKQ 337

BLAST of Carg25467 vs. Swiss-Prot
Match: sp|Q9M130|WTR29_ARATH (WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 6.0e-79
Identity = 154/308 (50.00%), Postives = 215/308 (69.81%), Query Frame = 0

Query: 2   NILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLGL 61
           N L+K++L+ G++ ++I  YR  IS+LFL PIA+FWERKTRP LT  IL  LF S+L+G 
Sbjct: 25  NALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWERKTRPTLTLNILVQLFFSALVGA 84

Query: 62  TLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVCI 121
           +LT Y FL+GL YTSAT +CAF+++ P  TFV+A++ R+EK++ KSK G   V+G L+CI
Sbjct: 85  SLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKLNMKSKAGMGMVMGALICI 144

Query: 122 SGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLMQ 181
            G LLL +YKG+PLT       +T+       A  P  W+IG ++L AG   + SW L+Q
Sbjct: 145 GGALLLTMYKGVPLTK--LRKLETHQLINNNHAMKPENWIIGCVLLFAGSSCFGSWMLIQ 204

Query: 182 SRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSGL 241
           ++V + YPCQYSST ++SFF  IQ A+L LI  R  + +I+     I+++VYAG+V  G+
Sbjct: 205 AKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILTDKLDIVTIVYAGAVAQGI 264

Query: 242 CYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILLW 301
           C VG SWC++++GP+FT+ FTP   +FA +FDF ILH QI LGSV+GS ++I G+YI L 
Sbjct: 265 CTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFLGSVVGSGVVIFGLYIFLL 324

Query: 302 GKDREAKE 310
           GK R  KE
Sbjct: 325 GKVRLMKE 330

BLAST of Carg25467 vs. Swiss-Prot
Match: sp|Q9M129|WTR30_ARATH (WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.5e-71
Identity = 142/312 (45.51%), Postives = 207/312 (66.35%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN L+K++L+ GI+ ++I  YR  IS+LFLLP+AYFWERKTRP+LT  I   LF+S+L G
Sbjct: 24  VNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWERKTRPKLTLSISCQLFVSALFG 83

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
            +L  Y +L+GL YTSAT   AF  ++P  TFV+A++   EK+  K+K G   VLGTL+ 
Sbjct: 84  ASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKLSLKTKIGYGVVLGTLIS 143

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           + G LLL +Y+G+PLT+     A +N             W+ G   L  G  ++SSW L+
Sbjct: 144 LVGGLLLTMYQGIPLTNSPEQAANSN------NHTGHENWIKGCFFLLTGVVLFSSWMLI 203

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+++   YPC YSST I+S F  +Q A+L LI  R    +I++    I+++V AG V  G
Sbjct: 204 QAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWILRDELTIITVVIAGVVAQG 263

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           +C VGMSWC+KQ+GPV +++F+P + + A +FDF ILH +I+LGSVIGSV+++ G+YI L
Sbjct: 264 MCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIYLGSVIGSVVVVIGLYIFL 323

Query: 301 WGKDREAKECAV 313
           W + ++  EC +
Sbjct: 324 WSRSKQIVECKI 329

BLAST of Carg25467 vs. Swiss-Prot
Match: sp|Q9M131|WTR28_ARATH (WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 7.9e-63
Identity = 139/328 (42.38%), Postives = 195/328 (59.45%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN L+K+ L+ G++ ++   YR  IS+L L+P +Y WERKTRPQLT  +L   FIS LLG
Sbjct: 23  VNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWERKTRPQLTFMLLCEHFISGLLG 82

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKV-DTKSKGGRAKVLGTLV 120
            +L  + FL+GL YTSAT S A ++++P  TF LA++ R+E   + KSK G  KV+GTL+
Sbjct: 83  ASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIFRIENAQNLKSKAGVLKVMGTLI 142

Query: 121 CISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFL 180
           CI G +LL  YKG  L++P S              D   KWL+G L L  G  + S W L
Sbjct: 143 CIMGAMLLTFYKGPELSNPHSHPQARXXXXXXXXXDQTKKWLLGCLYLVIGTVLLSLWML 202

Query: 181 MQSRVGKVYP-CQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVG 240
            Q ++   YP  +YSSTC+MS F++ Q A+L L   R    +I++  F IL  +YAG VG
Sbjct: 203 FQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRDVKDWIIEDKFVILVTLYAGIVG 262

Query: 241 SGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYI 300
             +  V  SW +K  G VF + F+P   V A +FDF ILH  ++LGS++GSV+ I+G+Y+
Sbjct: 263 QAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLILHSPLYLGSILGSVVTITGLYV 322

Query: 301 LLWGKDREAKECAVNQRVSVEEGYRKSN 327
            LWG+  E  +       S +    K N
Sbjct: 323 FLWGRKNETDQSVSKTLNSSQFSQNKDN 350

BLAST of Carg25467 vs. Swiss-Prot
Match: sp|Q5XEZ0|WTR1_ARATH (WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-62
Identity = 142/337 (42.14%), Postives = 199/337 (59.05%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN L+K+ L+ G++ ++I  YR  IS+L L+P AY  ERKTRPQ+T  ++   F+S LLG
Sbjct: 30  VNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRPQITFRLMVDHFVSGLLG 89

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVD-TKSKGGRAKVLGTLV 120
            +L  + FL+GL YTSAT SCA ++++P  TF LA++ R E V   K+K G  KV+GTL+
Sbjct: 90  ASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENVKILKTKAGMLKVIGTLI 149

Query: 121 CISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFL 180
           CISG L L  YKG P  S                      WL+G L LT G  + S W L
Sbjct: 150 CISGALFLTFYKG-PQISNXXXXXXXXXXXXXXXXXXANNWLLGCLYLTIGTVLLSLWML 209

Query: 181 MQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGS 240
            Q  +   YPC+YSSTC+MS F+A Q A+L L   R  + +I+   F I  ++YAG VG 
Sbjct: 210 FQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWIIDDRFVITVIIYAGVVGQ 269

Query: 241 GLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYIL 300
            +  V  +W +K+ G VF +AF P   + A +FDF ILH  ++LGSVIGS++ I+G+Y+ 
Sbjct: 270 AMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLYLGSVIGSLVTITGLYMF 329

Query: 301 LWGKDREAK-ECAVNQRVSVEEGYRKSNIPTTSTDAV 336
           LWGK++E +   A++  +  E  Y   N    S   V
Sbjct: 330 LWGKNKETESSTALSSGMDNEAQYTTPNKDNDSKSPV 365

BLAST of Carg25467 vs. TrEMBL
Match: tr|A0A1S3BCC0|A0A1S3BCC0_CUCME (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488142 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 5.5e-162
Identity = 296/338 (87.57%), Postives = 315/338 (93.20%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT Y LFLLFISSLLG
Sbjct: 17  VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYTLFLLFISSLLG 76

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLTLYLFLIGLH+TSATFSCAFLNLVPVNTF LAVL RMEKV+ KSKGG AKV+GTLVC
Sbjct: 77  LTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFFLAVLFRMEKVNMKSKGGIAKVVGTLVC 136

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           ISGNLLLILYKG PLTSPGS   +TNG R  V+A+T GKWLIGSLVLT GCFMWSSWFLM
Sbjct: 137 ISGNLLLILYKGTPLTSPGSTNGKTNGGRAMVEAETQGKWLIGSLVLTGGCFMWSSWFLM 196

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+RVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNS+FIVKG FAILSL+YAGSVGSG
Sbjct: 197 QTRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSLFIVKGKFAILSLIYAGSVGSG 256

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHE IHLGSV+GSV+IISGMYILL
Sbjct: 257 LCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEPIHLGSVVGSVLIISGMYILL 316

Query: 301 WGKDREAKECAVNQRVSVEEGYRKSNIPTTSTDAVEKI 339
           WGKD+EAKEC V Q +S+EE Y KSNI +T+TDA+E+I
Sbjct: 317 WGKDKEAKECVVKQTISIEEAYHKSNISSTNTDALEQI 354

BLAST of Carg25467 vs. TrEMBL
Match: tr|A0A0A0M167|A0A0A0M167_CUCSA (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G538830 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 8.6e-139
Identity = 258/284 (90.85%), Postives = 270/284 (95.07%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT YILFLLFISSLLG
Sbjct: 17  VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFISSLLG 76

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLTLYLFLIGLH+TSATFSCAFLNLVPVNTF+LAVL RMEKV+ KSKGG AKV+GTLVC
Sbjct: 77  LTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNMKSKGGIAKVVGTLVC 136

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           ISGNLLLILYKG PLTSPGS   +TNG R TV+A T GKWLIGSLVLT GCFMWSSWFLM
Sbjct: 137 ISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSLVLTGGCFMWSSWFLM 196

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+RVG+VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNS+FIVKG FAILSL+YAGSVGSG
Sbjct: 197 QTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGKFAILSLIYAGSVGSG 256

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLG 285
           LCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHEQIHLG
Sbjct: 257 LCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300

BLAST of Carg25467 vs. TrEMBL
Match: tr|A0A2R6R7G7|A0A2R6R7G7_ACTCH (WAT1-related protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc08322 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 5.3e-104
Identity = 193/310 (62.26%), Postives = 247/310 (79.68%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VNILLK++LNEGIS LLI+ YRQ+IS++FL P+AYFWERK RP+LT  IL  LF S+L+G
Sbjct: 23  VNILLKKVLNEGISHLLIITYRQSISAIFLAPVAYFWERKGRPKLTVLILCHLFFSALVG 82

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
            TLT Y FL+GL YTSATF+C+F+N+VPVNTF++A+   +EKV+ K+KGGRAK+LG LV 
Sbjct: 83  ATLTQYFFLLGLQYTSATFTCSFINMVPVNTFIMALPFGLEKVNMKNKGGRAKILGMLVS 142

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           + G L+L LYKG+PLT   SA A  +      +     +W IGS+VLTAG  MWSSWFLM
Sbjct: 143 VGGALILTLYKGIPLTKSPSARAIVHKRHHGTK-----RWAIGSIVLTAGSIMWSSWFLM 202

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+++GK YPCQYSST +MSFFSA+QSA+L LI D   S + +KG F I ++VYAG VGSG
Sbjct: 203 QAKIGKRYPCQYSSTALMSFFSAVQSAILVLITDWNASKWTIKGKFEITTVVYAGIVGSG 262

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYV MSWCVKQ+GPVFTAAF+PF++ F A+FD  ILHE++H+GSV+GS ++++GMYILL
Sbjct: 263 LCYVVMSWCVKQRGPVFTAAFSPFIQAFVAVFDISILHEEVHIGSVLGSTLVVAGMYILL 322

Query: 301 WGKDREAKEC 311
           WGK  EA++C
Sbjct: 323 WGKSNEAEDC 327

BLAST of Carg25467 vs. TrEMBL
Match: tr|B9I970|B9I970_POPTR (WAT1-related protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G108500v3 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 4.9e-102
Identity = 187/320 (58.44%), Postives = 248/320 (77.50%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN+LLK+++++G + ++I  YR + S++FL PIAYFWERK RP+LT  I   LF+ + +G
Sbjct: 23  VNLLLKKMIDQGTNNMVIATYRLSSSAIFLAPIAYFWERKGRPKLTASIFCHLFLGAFVG 82

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           LTLT YLFL+GL Y S TFSCAFLN VPVNTF+LA+L R+EK++  SK GRAKVLGT +C
Sbjct: 83  LTLTQYLFLLGLEYISVTFSCAFLNTVPVNTFILALLFRLEKLNMSSKAGRAKVLGTFIC 142

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQADTPGKWLIGSLVLTAGCFMWSSWFLM 180
           + G +LLILYKG+PLT+P S    T+   + +       W+ GS++  AGCFMWS+WFLM
Sbjct: 143 MGGAVLLILYKGIPLTNPRSEATTTHD--ILISGKKKRSWVAGSILSLAGCFMWSAWFLM 202

Query: 181 QSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGSG 240
           Q+++ K+YPCQYSST IMSFF AIQSA L  I+ RK S++I+K    I+S++ AG +GSG
Sbjct: 203 QAKISKIYPCQYSSTAIMSFFGAIQSAALSSILKRKFSMWILKSKLEIISVLNAGIIGSG 262

Query: 241 LCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYILL 300
           LCYVGMSWC+KQ+GPVFT+AFTPF+++FAA+FDF ILHEQI+LGSV+GS+++I G+YILL
Sbjct: 263 LCYVGMSWCIKQRGPVFTSAFTPFIQIFAAMFDFSILHEQIYLGSVLGSIVVILGLYILL 322

Query: 301 WGKDREAKECAVNQRVSVEE 321
           WGK  EA +C   Q    +E
Sbjct: 323 WGKRTEAVDCGEKQAQLTDE 340

BLAST of Carg25467 vs. TrEMBL
Match: tr|W9S3Y4|W9S3Y4_9ROSA (WAT1-related protein OS=Morus notabilis OX=981085 GN=L484_019587 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.9e-101
Identity = 193/322 (59.94%), Postives = 251/322 (77.95%), Query Frame = 0

Query: 1   VNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTPYILFLLFISSLLG 60
           VN+LLK+ L++G++ L+IV YRQ IS++FL PIA FWERK+  ++T  +L  LF S+L+G
Sbjct: 23  VNLLLKKTLDQGLNYLIIVTYRQAISAIFLTPIACFWERKSGTEITSRLLCYLFFSALVG 82

Query: 61  LTLTLYLFLIGLHYTSATFSCAFLNLVPVNTFVLAVLLRMEKVDTKSKGGRAKVLGTLVC 120
           +TLT +LFL+GL  TSATFSCAFLN+VPV TF+LA+ L +EK++ +SK GRAK+LGTL+C
Sbjct: 83  VTLTQFLFLLGLENTSATFSCAFLNMVPVFTFILALPLGLEKLNLESKSGRAKILGTLIC 142

Query: 121 ISGNLLLILYKGMPLTSPGSATAQTNGARVTVQA-DTPGKWLIGSLVLTAGCFMWSSWFL 180
           I G L+L  Y+G  LT P S   Q  G  V   +   P  W +G+++LTAGC MWSSWFL
Sbjct: 143 ILGALILTFYRGTQLTHPHS---QAVGNIVNANSPQKPKNWALGAVLLTAGCLMWSSWFL 202

Query: 181 MQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSVFIVKGTFAILSLVYAGSVGS 240
           MQ+R+GK +P QYSST I+SFF AIQSA+L LI++R  S++I+KG   I+S+VYAG VGS
Sbjct: 203 MQARIGKSFPFQYSSTAIISFFGAIQSAILSLILERNLSMWILKGKLEIISVVYAGMVGS 262

Query: 241 GLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHEQIHLGSVIGSVMIISGMYIL 300
            LCYVGMSWCVK KGPVFT+AFTPF ++F AIFDF I HEQ++LGS++GS ++I GMY L
Sbjct: 263 ALCYVGMSWCVKHKGPVFTSAFTPFTQIFVAIFDFSIFHEQVYLGSLLGSALVIIGMYTL 322

Query: 301 LWGKDREAKECAVNQRVSVEEG 322
           LWGK++EAKEC V Q  + EEG
Sbjct: 323 LWGKNKEAKECLVKQSQTTEEG 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022927181.14.4e-17998.22WAT1-related protein At3g30340-like isoform X3 [Cucurbita moschata][more]
XP_022927182.14.4e-17998.22WAT1-related protein At3g30340-like isoform X4 [Cucurbita moschata][more]
XP_022927179.14.4e-17998.22WAT1-related protein At3g30340-like isoform X1 [Cucurbita moschata][more]
XP_023520075.12.4e-17797.34WAT1-related protein At3g30340-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_023520074.12.4e-17797.34WAT1-related protein At3g30340-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G30340.11.1e-8651.59nodulin MtN21 /EamA-like transporter family protein[more]
AT4G01440.13.3e-8050.00nodulin MtN21 /EamA-like transporter family protein[more]
AT4G01450.22.0e-7245.51nodulin MtN21 /EamA-like transporter family protein[more]
AT4G01430.14.4e-6442.38nodulin MtN21 /EamA-like transporter family protein[more]
AT1G01070.17.5e-6442.14nodulin MtN21 /EamA-like transporter family protein[more]
Match NameE-valueIdentityDescription
sp|Q9LI65|WTR24_ARATH1.9e-8551.59WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2... [more]
sp|Q9M130|WTR29_ARATH6.0e-7950.00WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2... [more]
sp|Q9M129|WTR30_ARATH3.5e-7145.51WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2... [more]
sp|Q9M131|WTR28_ARATH7.9e-6342.38WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2... [more]
sp|Q5XEZ0|WTR1_ARATH1.3e-6242.14WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BCC0|A0A1S3BCC0_CUCME5.5e-16287.57WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488142 PE=3 SV=1[more]
tr|A0A0A0M167|A0A0A0M167_CUCSA8.6e-13990.85WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G538830 PE=3 SV=1[more]
tr|A0A2R6R7G7|A0A2R6R7G7_ACTCH5.3e-10462.26WAT1-related protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_A... [more]
tr|B9I970|B9I970_POPTR4.9e-10258.44WAT1-related protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G108500v3 PE=3 S... [more]
tr|W9S3Y4|W9S3Y4_9ROSA1.9e-10159.94WAT1-related protein OS=Morus notabilis OX=981085 GN=L484_019587 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR030184WAT1-related
IPR000620EamA_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25467-RACarg25467-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 7..123
e-value: 1.8E-13
score: 50.8
coord: 162..300
e-value: 2.3E-13
score: 50.4
IPR030184WAT1-related proteinPANTHERPTHR31218FAMILY NOT NAMEDcoord: 1..324
NoneNo IPR availablePANTHERPTHR31218:SF10SUBFAMILY NOT NAMEDcoord: 1..324
NoneNo IPR availableSUPERFAMILYSSF103481Multidrug resistance efflux transporter EmrEcoord: 21..131
NoneNo IPR availableSUPERFAMILYSSF103481Multidrug resistance efflux transporter EmrEcoord: 225..305