Carg25254 (gene) Silver-seed gourd

Overview
NameCarg25254
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionTransmembrane ascorbate ferrireductase 1
LocationCucurbita_argyrosperma_scaffold_210 : 170049 .. 170957 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACACTGTGAACAGGCCAGGTTTCAGGCATACTGCCTCTGCCATTGCAGCCCTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTACGCTTGTTTGGCTCCTGCATTATCGAGGCGGTCTCGCCTACGATTCTCGGAGTGCCGACCTCGTTTTCAATGTAAGGCTCACATTTAATTTGTAGTTTTAAAGGAACTGATATTAAGAGATTTACTTTTGGCAGTGCCACCCGTTCTTCATGCTCCTTGGTTTCGTGATCGTGTCGGGGGAAGGTATAGCTCGATCGAGTTTGTTTAATTTATATCTTGAGATGATTCATAACTATAATTGAATTGGATTTGAGCAGCAATGATGGCTTACAAGACAGTGAGGGCAGACAAAATGACAAGGAAGATAGTTCATATGGCGTTGCATTTTGTAGCCATTTGTATGGGAATTGTAGGCATAAATGCTTCTTTCAAGTTCCATGATATGATCAAATTGGAGCATATGTTTAGTTTGCATTCGTGGATTGGCATTGTCACTTTCTGCCTCTATGGCTTACAGGTACATAATATAATATAATATGCTGTTTGTCGTTAAAGTTTCAATTATGTTCTTAAACTTTTATACACGAATGGTATGGCAGTGGCTGTTGGGTCTGTGCACGTTCTTGTTTCCAGGAGCAGGCAGTGAGACCAAAGCCAGGCTGCTTCCATGGCACATAAGTGGGGGAAGGGCTCTTCTGCTGATGATTATCTGCACAGCCTTGACTGGTTTGTCTCAGAAGGCTACTTCTTCGACACTCACTGGCAACCATGAGGCTCGTTTGCTTAAGTTCACTGGTTTCTTCGTCCTCCTCTTCGGCATTTCCATCGATATCGCCATCGGTTTTGATCGGTTTCGCCACGTCGATCCCACGACTTAA

mRNA sequence

ATGGACACTGTGAACAGGCCAGGTTTCAGGCATACTGCCTCTGCCATTGCAGCCCTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTACGCTTGTTTGGCTCCTGCATTATCGAGGCGGTCTCGCCTACGATTCTCGGAGTGCCGACCTCGTTTTCAATTGCCACCCGTTCTTCATGCTCCTTGGTTTCGTGATCGTGTCGGGGGAAGCAATGATGGCTTACAAGACAGTGAGGGCAGACAAAATGACAAGGAAGATAGTTCATATGGCGTTGCATTTTGTAGCCATTTGTATGGGAATTGTAGGCATAAATGCTTCTTTCAAGTTCCATGATATGATCAAATTGGAGCATATGTTTAGTTTGCATTCGTGGATTGGCATTGTCACTTTCTGCCTCTATGGCTTACAGTGGCTGTTGGGTCTGTGCACGTTCTTGTTTCCAGGAGCAGGCAGTGAGACCAAAGCCAGGCTGCTTCCATGGCACATAAGTGGGGGAAGGGCTCTTCTGCTGATGATTATCTGCACAGCCTTGACTGGTTTGTCTCAGAAGGCTACTTCTTCGACACTCACTGGCAACCATGAGGCTCGTTTGCTTAAGTTCACTGGTTTCTTCGTCCTCCTCTTCGGCATTTCCATCGATATCGCCATCGGTTTTGATCGGTTTCGCCACGTCGATCCCACGACTTAA

Coding sequence (CDS)

ATGGACACTGTGAACAGGCCAGGTTTCAGGCATACTGCCTCTGCCATTGCAGCCCTTGCACATATTTTTGGTCTCACTGCCTGCATTCTTACGCTTGTTTGGCTCCTGCATTATCGAGGCGGTCTCGCCTACGATTCTCGGAGTGCCGACCTCGTTTTCAATTGCCACCCGTTCTTCATGCTCCTTGGTTTCGTGATCGTGTCGGGGGAAGCAATGATGGCTTACAAGACAGTGAGGGCAGACAAAATGACAAGGAAGATAGTTCATATGGCGTTGCATTTTGTAGCCATTTGTATGGGAATTGTAGGCATAAATGCTTCTTTCAAGTTCCATGATATGATCAAATTGGAGCATATGTTTAGTTTGCATTCGTGGATTGGCATTGTCACTTTCTGCCTCTATGGCTTACAGTGGCTGTTGGGTCTGTGCACGTTCTTGTTTCCAGGAGCAGGCAGTGAGACCAAAGCCAGGCTGCTTCCATGGCACATAAGTGGGGGAAGGGCTCTTCTGCTGATGATTATCTGCACAGCCTTGACTGGTTTGTCTCAGAAGGCTACTTCTTCGACACTCACTGGCAACCATGAGGCTCGTTTGCTTAAGTTCACTGGTTTCTTCGTCCTCCTCTTCGGCATTTCCATCGATATCGCCATCGGTTTTGATCGGTTTCGCCACGTCGATCCCACGACTTAA

Protein sequence

MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Homology
[Close]
BLAST of Carg25254 vs. NCBI nr
Match: XP_022961824.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita moschata])

HSP 1 Score: 464.9 bits (1195), Expect = 1.6e-127
Identity = 228/229 (99.56%), Postives = 229/229 (100.00%), Query Frame = 0 

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM
Sbjct: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF
Sbjct: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           S+HSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SIHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 230
           LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Sbjct: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 229

[Close]
BLAST of Carg25254 vs. NCBI nr
Match: XP_023521256.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.7 bits (1153), Expect = 1.2e-122
Identity = 221/229 (96.51%), Postives = 225/229 (98.25%), Query Frame = 0 

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM
Sbjct: 13  MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 72

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFVIVSGEA+MAYKTVRADKMT KIVHMALHFVAICMGIVGINA+FKFHDMIKLEHMF
Sbjct: 73  LLGFVIVSGEAIMAYKTVRADKMTTKIVHMALHFVAICMGIVGINAAFKFHDMIKLEHMF 132

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGLCTFLF GA  ETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 133 SLHSWIGIVTFCLYGLQWLLGLCTFLFTGATRETKARLLPWHISGGRALLLMIICTALTG 192

Query: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 230
           LSQKATSSTLTGN+E+RLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT
Sbjct: 193 LSQKATSSTLTGNNESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 241

[Close]
BLAST of Carg25254 vs. NCBI nr
Match: XP_022991076.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima])

HSP 1 Score: 443.4 bits (1139), Expect = 4.8e-121
Identity = 217/229 (94.76%), Postives = 221/229 (96.51%), Query Frame = 0 

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MDTV+RPGFRHTASAIA LAHIFGLTACILTLVWLLHYRGGLAYDSRSA+LVFNCHPFFM
Sbjct: 1   MDTVDRPGFRHTASAIAVLAHIFGLTACILTLVWLLHYRGGLAYDSRSAELVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV VSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGI GINASFKFHDMIKLEH+F
Sbjct: 61  LLGFVFVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIAGINASFKFHDMIKLEHVF 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGLCTFLFPGA  ETKARLLPWHISGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGASRETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPTT 230
           LSQKATSSTLTGNHE+RLL FTGFFVLLFGISIDIAIGFDRFRH D TT
Sbjct: 181 LSQKATSSTLTGNHESRLLNFTGFFVLLFGISIDIAIGFDRFRHADLTT 229

[Close]
BLAST of Carg25254 vs. NCBI nr
Match: XP_004142817.1 (PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis sativus] >KGN51448.1 hypothetical protein Csa_5G548140 [Cucumis sativus])

HSP 1 Score: 378.6 bits (971), Expect = 1.5e-101
Identity = 182/228 (79.82%), Postives = 201/228 (88.16%), Query Frame = 0 

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MD + R  FRHTAS I   AHIFGLTACILTLVWLLHYRGGLAYDS +++LVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV +SGEAMMAY+TVRA+K T+KIVHMALH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GL TFLFPGA  ETK RLLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADRETKTRLLPWHITGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 229
           LS+KATS  LT +HE+RLL FT FFVLLFGISIDI+IGF RF HVD T
Sbjct: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHT 228

[Close]
BLAST of Carg25254 vs. NCBI nr
Match: XP_008466953.1 (PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis melo])

HSP 1 Score: 374.0 bits (959), Expect = 3.6e-100
Identity = 181/228 (79.39%), Postives = 198/228 (86.84%), Query Frame = 0 

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MD + R  FRHTAS IA  AHI GLTACIL LVWLLHYRGGLAYDS  A+LVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTIAVFAHISGLTACILMLVWLLHYRGGLAYDSPDANLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV +SGEAMMAY+TVRA+K+T+KIVHM LH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKITKKIVHMGLHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGL TFLFPGA + TK RLLPWHISGGRALL+MIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLFTFLFPGADAMTKTRLLPWHISGGRALLMMIICTALTG 180

Query: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 229
           LS+KATS  LTG+HE+RL  FT FFVLLFGISIDI+I F RF HVD T
Sbjct: 181 LSEKATSLRLTGSHESRLFNFTAFFVLLFGISIDISISFARFMHVDHT 228

[Close]
BLAST of Carg25254 vs. TAIR10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 219.2 bits (557), Expect = 2.7e-57
Identity = 105/218 (48.17%), Postives = 143/218 (65.60%), Query Frame = 0 

Query: 5   NRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGF 64
           +R   +  +S    +AH FG+ A +L L+WLLHYR G+ Y S +   V N HPF M  GF
Sbjct: 6   DRTTLKRHSSLSTLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGF 65

Query: 65  VIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHS 124
           + + G+AMM YKT  A    +K+VH  LH + + +GIVGI A+F+FHD + L+ M SLHS
Sbjct: 66  LFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHS 125

Query: 125 WIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQK 184
           WIG+ TF L G+QWL G  TFL P + S T+ R++PWH+ GGRALL M I  ALTGL Q+
Sbjct: 126 WIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQR 185

Query: 185 ATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRF 223
           AT    + N E+RL+ F G  +LLFG+S+D ++   R+
Sbjct: 186 ATMLGQSTNAESRLINFLGLAILLFGVSVDFSVALGRY 223

[Close]
BLAST of Carg25254 vs. TAIR10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 169.5 bits (428), Expect = 2.4e-42
Identity = 82/205 (40.00%), Postives = 125/205 (60.98%), Query Frame = 0 

Query: 10  RHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSG 69
           R  A A+  +AH   + A I+ LVW + YRGGLA+++ + +L+FN HP  ML+GF+I+ G
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 70  EAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIV 129
           EA+++YK++  +K  +K++H+ LH +A+ +GI GI A+FK H+   + +++SLHSWIGI 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 130 TFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSST 189
              LYG QW+     F FPG  +  K+ LLPWH   G  + ++ +  A  G  +K T   
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 190 LTG----NHEARLLKFTGFFVLLFG 211
             G      EA L+ FT    +LFG
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFG 208

[Close]
BLAST of Carg25254 vs. TAIR10
Match: AT5G38630.1 (cytochrome B561-1)

HSP 1 Score: 151.8 bits (382), Expect = 5.3e-37
Identity = 72/200 (36.00%), Postives = 117/200 (58.50%), Query Frame = 0 

Query: 16  IAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAMMAY 75
           I  +  + G     L L W +HYRGGLA  S + D +FN HP  M++G ++ +GEAM+AY
Sbjct: 11  IFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLAY 70

Query: 76  KTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTFCLYG 135
           K+V+  K  +K+VH+ L   A  + ++G+ A+ KFH    +E+ +SLHSW+G+    L+ 
Sbjct: 71  KSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFA 130

Query: 136 LQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKAT----SSTLT 195
            QW  G  T+ +PG    ++A L+PWH+  G ++  + + TA TG+ +K T    +  +T
Sbjct: 131 FQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVIT 190

Query: 196 G-NHEARLLKFTGFFVLLFG 211
             + EA L+   G  +L+ G
Sbjct: 191 RYSTEAMLVNTMGVLILILG 210

[Close]
BLAST of Carg25254 vs. TAIR10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family)

HSP 1 Score: 96.3 bits (238), Expect = 2.6e-20
Identity = 54/173 (31.21%), Postives = 90/173 (52.02%), Query Frame = 0 

Query: 15  AIAALAHIFGLTACILTLVWLLHYRG-GLAYDSRSADLVFNCHPFFMLLGFVIVSGEAMM 74
           ++   A + GL   +  L W L     GL+Y +         HP  M++GF++VSGEA++
Sbjct: 10  SLVLFARLSGLVVAVSVLYWALFLPNLGLSYST--------LHPLLMVIGFILVSGEAIL 69

Query: 75  AYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTFCL 134
            ++ +   + T+K VH+ L  +A+   + GI   F +   +   + +SLHSW+G+++  L
Sbjct: 70  IHRWLPGSRKTKKAVHLWLQGMALASAVFGIWTKFHYQRGV-FANFYSLHSWMGLLSVSL 129

Query: 135 YGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKAT 187
           +  QW+ G  +F   G    T+   LPWH+  G     + I TA TGL +K T
Sbjct: 130 FAAQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLT 173

[Close]
BLAST of Carg25254 vs. Swiss-Prot
Match: sp|Q67ZF6|ACFR3_ARATH (Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=3702 GN=CYB561C PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 4.9e-56
Identity = 105/218 (48.17%), Postives = 143/218 (65.60%), Query Frame = 0 

Query: 5   NRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGF 64
           +R   +  +S    +AH FG+ A +L L+WLLHYR G+ Y S +   V N HPF M  GF
Sbjct: 6   DRTTLKRHSSLSTLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGF 65

Query: 65  VIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHS 124
           + + G+AMM YKT  A    +K+VH  LH + + +GIVGI A+F+FHD + L+ M SLHS
Sbjct: 66  LFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHS 125

Query: 125 WIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQK 184
           WIG+ TF L G+QWL G  TFL P + S T+ R++PWH+ GGRALL M I  ALTGL Q+
Sbjct: 126 WIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQR 185

Query: 185 ATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRF 223
           AT    + N E+RL+ F G  +LLFG+S+D ++   R+
Sbjct: 186 ATMLGQSTNAESRLINFLGLAILLFGVSVDFSVALGRY 223

[Close]
BLAST of Carg25254 vs. Swiss-Prot
Match: sp|A3A9H6|ACET1_ORYSJ (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 4.0e-42
Identity = 84/209 (40.19%), Postives = 128/209 (61.24%), Query Frame = 0 

Query: 13  ASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAM 72
           A+    +AH   + A  + LVW +H+RGGLA+++ + +L+FN HP  ML+G++I+  EA+
Sbjct: 7   AAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSEAI 66

Query: 73  MAYKTVRADKM-TRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTF 132
           M YK +   K  T K++H+ LH +A+  G VGI  +FKFH+   + +++SLHSW+GI T 
Sbjct: 67  MVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTI 126

Query: 133 CLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLT 192
           CLYG+QW+ G   F FP A    +  +LPWHI  G  + ++ + TA  G  +K T    +
Sbjct: 127 CLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQSS 186

Query: 193 G----NHEARLLKFTGFFVLLFGISIDIA 217
           G      EA L+ FT   V+LFG S+ +A
Sbjct: 187 GLDKYGAEAFLVNFTALIVVLFGASVVVA 215

[Close]
BLAST of Carg25254 vs. Swiss-Prot
Match: sp|Q8L856|ACFR1_ARATH (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 4.4e-41
Identity = 82/205 (40.00%), Postives = 125/205 (60.98%), Query Frame = 0 

Query: 10  RHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSG 69
           R  A A+  +AH   + A I+ LVW + YRGGLA+++ + +L+FN HP  ML+GF+I+ G
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 70  EAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIV 129
           EA+++YK++  +K  +K++H+ LH +A+ +GI GI A+FK H+   + +++SLHSWIGI 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 130 TFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSST 189
              LYG QW+     F FPG  +  K+ LLPWH   G  + ++ +  A  G  +K T   
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 190 LTG----NHEARLLKFTGFFVLLFG 211
             G      EA L+ FT    +LFG
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFG 208

[Close]
BLAST of Carg25254 vs. Swiss-Prot
Match: sp|Q7XMK3|ACET2_ORYSJ (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 169.1 bits (427), Expect = 5.8e-41
Identity = 82/209 (39.23%), Postives = 129/209 (61.72%), Query Frame = 0 

Query: 13  ASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAM 72
           A+    +AH   + A ++ LVW + +RGGLA+++ + +L+FN HP  ML+G++I+  EA+
Sbjct: 9   AAPFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSEAI 68

Query: 73  MAYKTV-RADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTF 132
           M YK   + +  T K++H+ LH +AI +G VGI  +FKFH+   + +++SLHSW+GI T 
Sbjct: 69  MIYKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIGTI 128

Query: 133 CLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLT 192
            LYG+QW+ G   F +PGA    +   LPWH+  G  + ++ + TA  GL +K T    +
Sbjct: 129 SLYGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQSS 188

Query: 193 G----NHEARLLKFTGFFVLLFGISIDIA 217
           G      EA L+ FTG  V LFG ++ +A
Sbjct: 189 GLDKYGAEAFLVNFTGLVVALFGAAVVVA 217

[Close]
BLAST of Carg25254 vs. Swiss-Prot
Match: sp|C4IYS8|ACFR2_MAIZE (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.5e-41
Identity = 83/209 (39.71%), Postives = 129/209 (61.72%), Query Frame = 0 

Query: 13  ASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVSGEAM 72
           A+     AH   + A  + LVW +++RGGLA ++ + +L+FN HP  ML+G++I+ GEA+
Sbjct: 9   AAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGEAI 68

Query: 73  MAYKTV-RADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGIVTF 132
           M Y+ +  ++  T K++H+ LH +A+ +G VGI  +FK H+   + +++SLHSWIGI T 
Sbjct: 69  MVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTI 128

Query: 133 CLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSSTLT 192
            LYG+QW++G  TF FPGA    K  +LPWHI  G  + ++ +  A  G  +K T    +
Sbjct: 129 TLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLESS 188

Query: 193 G----NHEARLLKFTGFFVLLFGISIDIA 217
           G      EA L+ FT   V+LFG S+ +A
Sbjct: 189 GLDKYGTEAFLVNFTALVVVLFGASVVVA 217

[Close]
BLAST of Carg25254 vs. TrEMBL
Match: tr|A0A0A0KPB5|A0A0A0KPB5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G548140 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 9.7e-102
Identity = 182/228 (79.82%), Postives = 201/228 (88.16%), Query Frame = 0 

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MD + R  FRHTAS I   AHIFGLTACILTLVWLLHYRGGLAYDS +++LVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV +SGEAMMAY+TVRA+K T+KIVHMALH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GL TFLFPGA  ETK RLLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADRETKTRLLPWHITGGRALLLMIICTALTG 180

Query: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 229
           LS+KATS  LT +HE+RLL FT FFVLLFGISIDI+IGF RF HVD T
Sbjct: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHT 228

[Close]
BLAST of Carg25254 vs. TrEMBL
Match: tr|A0A1S3CSD9|A0A1S3CSD9_CUCME (probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC103504338 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.4e-100
Identity = 181/228 (79.39%), Postives = 198/228 (86.84%), Query Frame = 0 

Query: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60
           MD + R  FRHTAS IA  AHI GLTACIL LVWLLHYRGGLAYDS  A+LVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTIAVFAHISGLTACILMLVWLLHYRGGLAYDSPDANLVFNCHPFFM 60

Query: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120
           LLGFV +SGEAMMAY+TVRA+K+T+KIVHM LH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKITKKIVHMGLHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWLLGL TFLFPGA + TK RLLPWHISGGRALL+MIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLFTFLFPGADAMTKTRLLPWHISGGRALLMMIICTALTG 180

Query: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 229
           LS+KATS  LTG+HE+RL  FT FFVLLFGISIDI+I F RF HVD T
Sbjct: 181 LSEKATSLRLTGSHESRLFNFTAFFVLLFGISIDISISFARFMHVDHT 228

[Close]
BLAST of Carg25254 vs. TrEMBL
Match: tr|B9HVR8|B9HVR8_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_010G102400v3 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.1e-68
Identity = 124/214 (57.94%), Postives = 160/214 (74.77%), Query Frame = 0 

Query: 9   FRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVS 68
           +R +AS +  +AH+FG+ A IL LVWLLHYRGG+ Y S + D VFN HPFFM  GF+ + 
Sbjct: 8   YRQSASRLTVVAHLFGILAFILMLVWLLHYRGGIEYHSDNPDRVFNAHPFFMFCGFIFLV 67

Query: 69  GEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSLHSWIGI 128
           GEAMMAYKTV +  + +K +HM LH +A+C+GIVGI A FK+HDMI  E ++SLHSWIG+
Sbjct: 68  GEAMMAYKTVLSRGIVKKSIHMILHLIALCLGIVGICAVFKYHDMIHAEDVYSLHSWIGL 127

Query: 129 VTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATSS 188
           VTFCL+ LQW+LG   F+FP A   T+A +LPWH+SGGRALL M IC ALTGL +K+T  
Sbjct: 128 VTFCLFCLQWVLGFSAFMFPKAAPSTRASMLPWHVSGGRALLYMAICAALTGLMEKSTFL 187

Query: 189 TLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRF 223
            L  + EARL+ FTG FVLLFGI +D+++   R+
Sbjct: 188 NLKNHREARLINFTGLFVLLFGIFVDLSVALARY 221

[Close]
BLAST of Carg25254 vs. TrEMBL
Match: tr|A0A1Q3ANB3|A0A1Q3ANB3_CEPFO (Cytochrom_B561 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_00779 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 5.4e-68
Identity = 124/220 (56.36%), Postives = 163/220 (74.09%), Query Frame = 0 

Query: 3   TVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLL 62
           ++NR  ++ +A  +  +AH+FG+ A IL LVWLLH+RGG+ YDS S++ VFN HPF M  
Sbjct: 4   SINRETYKMSAYHLTKVAHLFGILAIILMLVWLLHFRGGIEYDSDSSNRVFNVHPFLMFC 63

Query: 63  GFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMFSL 122
           GF+ +SGEA+MAYKTVRA +  +K VHM L+  +IC+GIVGI A FKFHDM+ +E ++SL
Sbjct: 64  GFIFLSGEAIMAYKTVRATRNVQKYVHMILNLCSICLGIVGICAVFKFHDMVNVEDVYSL 123

Query: 123 HSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLS 182
           HSWIGI TFC++GLQWL GL TF+FP A    +A +LPWH+ GGRA+  M IC ALTGL 
Sbjct: 124 HSWIGISTFCMFGLQWLFGLVTFMFPKASPNARAMMLPWHVCGGRAIFFMAICAALTGLM 183

Query: 183 QKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRF 223
           +KAT S L    EARL+ FTG  +LLFGI +D+++   RF
Sbjct: 184 EKATFSGLQHQPEARLINFTGLSILLFGIFVDLSVALARF 223

[Close]
BLAST of Carg25254 vs. TrEMBL
Match: tr|A0A2I4FEQ8|A0A2I4FEQ8_9ROSI (probable transmembrane ascorbate ferrireductase 3 OS=Juglans regia OX=51240 GN=LOC108998128 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.5e-65
Identity = 122/216 (56.48%), Postives = 160/216 (74.07%), Query Frame = 0 

Query: 9   FRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFMLLGFVIVS 68
           ++ +AS +  +AH+FGL + +L LVWLLHY+GGL YDS  A  VFN HPF M  GF+ ++
Sbjct: 14  YQGSASRVTIVAHLFGLLSLVLILVWLLHYQGGLEYDSNEAYRVFNVHPFLMFFGFIFMA 73

Query: 69  GEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMI-KLEHMFSLHSWIG 128
           GEAMMA++TV A++  RK +HM  H +A+C+GIVGI A FKFH+MI     ++SLHSWIG
Sbjct: 74  GEAMMAFRTVAAEREVRKFIHMIFHLIALCLGIVGIKAVFKFHEMINNSPDVYSLHSWIG 133

Query: 129 IVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTGLSQKATS 188
           I TF L+ LQWL+G  TF+FP A   T+AR LPWH+SGGRALL M IC ALTGL +KAT 
Sbjct: 134 IGTFSLFCLQWLIGFVTFMFPRASHPTRARFLPWHMSGGRALLYMAICAALTGLMEKATF 193

Query: 189 STLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFR 224
             L  ++EAR++ FTG F+LLFGI +D++I   R+R
Sbjct: 194 LKLQHSYEARVINFTGLFILLFGIFVDLSIALGRYR 229

The following BLAST results are available for this feature:
BLAST of Carg25254 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022961824.11.6e-12799.56probable transmembrane ascorbate ferrireductase 3 [Cucurbita moschata][more]
XP_023521256.11.2e-12296.51probable transmembrane ascorbate ferrireductase 3 [Cucurbita pepo subsp. pepo][more]
XP_022991076.14.8e-12194.76probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima][more]
XP_004142817.11.5e-10179.82PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis sativus] >... [more]
XP_008466953.13.6e-10079.39PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis melo][more]
BLAST of Carg25254 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G14730.12.7e-5748.17Cytochrome b561/ferric reductase transmembrane protein family[more]
AT4G25570.12.4e-4240.00Cytochrome b561/ferric reductase transmembrane protein family[more]
AT5G38630.15.3e-3736.00cytochrome B561-1[more]
AT1G26100.12.6e-2031.21Cytochrome b561/ferric reductase transmembrane protein family[more]
BLAST of Carg25254 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q67ZF6|ACFR3_ARATH4.9e-5648.17Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=370... [more]
sp|A3A9H6|ACET1_ORYSJ4.0e-4240.19Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa... [more]
sp|Q8L856|ACFR1_ARATH4.4e-4140.00Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5... [more]
sp|Q7XMK3|ACET2_ORYSJ5.8e-4139.23Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa... [more]
sp|C4IYS8|ACFR2_MAIZE7.5e-4139.71Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2... [more]
BLAST of Carg25254 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KPB5|A0A0A0KPB5_CUCSA9.7e-10279.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G548140 PE=4 SV=1[more]
tr|A0A1S3CSD9|A0A1S3CSD9_CUCME2.4e-10079.39probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC... [more]
tr|B9HVR8|B9HVR8_POPTR3.1e-6857.94Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_010G102400v3 PE=... [more]
tr|A0A1Q3ANB3|A0A1Q3ANB3_CEPFO5.4e-6856.36Cytochrom_B561 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=C... [more]
tr|A0A2I4FEQ8|A0A2I4FEQ8_9ROSI1.5e-6556.48probable transmembrane ascorbate ferrireductase 3 OS=Juglans regia OX=51240 GN=L... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006593Cyt_b561/ferric_Rdtase_TM
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25254-RACarg25254-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006593Cytochrome b561/ferric reductase transmembraneSMARTSM00665561_7coord: 55..181
e-value: 2.9E-43
score: 159.7
IPR006593Cytochrome b561/ferric reductase transmembranePFAMPF03188Cytochrom_B561coord: 56..186
e-value: 2.3E-37
score: 128.1
IPR006593Cytochrome b561/ferric reductase transmembranePROSITEPS50939CYTOCHROME_B561coord: 19..218
score: 42.737
NoneNo IPR availableGENE3DG3DSA:1.20.120.1770coord: 10..224
e-value: 1.6E-69
score: 235.6
NoneNo IPR availablePANTHERPTHR10106CYTOCHROME B561-RELATEDcoord: 12..222
NoneNo IPR availablePANTHERPTHR10106:SF15TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 3-RELATEDcoord: 12..222
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..28
score: 5.0
NoneNo IPR availableCDDcd08766Cyt_b561_ACYB-1_likecoord: 46..186
e-value: 3.55843E-60
score: 187.514