Carg25117 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25117
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cytosolic Fe-S cluster assembly factor narfl
Location	Cucurbita_argyrosperma_scaffold_211 : 78921 .. 79321 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGAGTTGCGATTCCCCTTGTTCTGCTTTATTTGTTTTGTTAACTCTTGAATTTGAATTCGGTGATTAGGAGATGTCTGAGAAATTCTCGGCAACATTGAGGATCGGGGATCTGAATGATTATATAGCGCCTTCCCAGGCTTGCATAGTTTCTCTCAAGGGGCTTAAAGCCACTTCCACGAAGCCGGATAAAGTTGAGGTACCTCTTCAATTCGCTTAAAAGTTGCAGCACATTGTATCCTTTTAAAACTGACCGAAAGAGATTCTTTCAGAAATTTTCTGTTCATTGATTTGTCTTCTTCATTTCTGCGTATGTTATCTAGGTTTCAGCTTCTAGAAAGCAATTGAAAGCTGAACCAGTTAAGATATCACTCAAGGATTGCTTAGCATGCAGGTAA

mRNA sequence

ATGGAGATGTCTGAGAAATTCTCGGCAACATTGAGGATCGGGGATCTGAATGATTATATAGCGCCTTCCCAGGCTTGCATAGTTTCTCTCAAGGGGCTTAAAGCCACTTCCACGAAGCCGGATAAAGTTGAGGTTTCAGCTTCTAGAAAGCAATTGAAAGCTGAACCAGTTAAGATATCACTCAAGGATTGCTTAGCATGCAGGTAA

Coding sequence (CDS)

ATGGAGATGTCTGAGAAATTCTCGGCAACATTGAGGATCGGGGATCTGAATGATTATATAGCGCCTTCCCAGGCTTGCATAGTTTCTCTCAAGGGGCTTAAAGCCACTTCCACGAAGCCGGATAAAGTTGAGGTTTCAGCTTCTAGAAAGCAATTGAAAGCTGAACCAGTTAAGATATCACTCAAGGATTGCTTAGCATGCAGGTAA

Protein sequence

MEMSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLKDCLACR

Homology

[Close]

BLAST of Carg25117 vs. NCBI nr
Match: XP_023001117.1 (protein NAR1-like [Cucurbita maxima])

HSP 1 Score: 126.7 bits (317), Expect = 3.0e-26
Identity = 65/65 (100.00%), Postives = 65/65 (100.00%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK
Sbjct: 1  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 65

[Close]

BLAST of Carg25117 vs. NCBI nr
Match: XP_022927572.1 (protein NAR1-like [Cucurbita moschata] >XP_022927574.1 protein NAR1-like [Cucurbita moschata])

HSP 1 Score: 125.2 bits (313), Expect = 8.7e-26
Identity = 64/65 (98.46%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVS SRKQLKAEPVKISLK
Sbjct: 1  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSTSRKQLKAEPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 65

[Close]

BLAST of Carg25117 vs. NCBI nr
Match: XP_023520381.1 (protein NAR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 124.4 bits (311), Expect = 1.5e-25
Identity = 64/65 (98.46%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQ KAEPVKISLK
Sbjct: 1  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQSKAEPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 65

[Close]

BLAST of Carg25117 vs. NCBI nr
Match: XP_022142658.1 (protein NAR1 [Momordica charantia])

HSP 1 Score: 124.0 bits (310), Expect = 1.9e-25
Identity = 63/65 (96.92%), Postives = 65/65 (100.00%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFSATLRIGDLND+IAPSQACIVSLKGLKAT+TKPDKVEVSASRKQLKAEPVKISLK
Sbjct: 1  MSEKFSATLRIGDLNDFIAPSQACIVSLKGLKATTTKPDKVEVSASRKQLKAEPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 65

[Close]

BLAST of Carg25117 vs. NCBI nr
Match: KGN65029.1 (hypothetical protein Csa_1G181420 [Cucumis sativus])

HSP 1 Score: 123.6 bits (309), Expect = 2.5e-25
Identity = 63/66 (95.45%), Postives = 65/66 (98.48%), Query Frame = 0


Query: 2   EMSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISL 61
           EMSEKFSATLRIGDLND+IAPSQACIVSLKGLKAT+TKPDKVEVSASR QLKAEPVKISL
Sbjct: 134 EMSEKFSATLRIGDLNDFIAPSQACIVSLKGLKATATKPDKVEVSASRMQLKAEPVKISL 193

Query: 62  KDCLAC 68
           KDCLAC
Sbjct: 194 KDCLAC 199

[Close]

BLAST of Carg25117 vs. TAIR10
Match: AT4G16440.1 (ferredoxin hydrogenases)

HSP 1 Score: 82.4 bits (202), Expect = 1.2e-16
Identity = 43/65 (66.15%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFS TLR+GDLND+IAPSQAC++SLK  K    K D+ +V  + KQ + EPVKISLK
Sbjct: 1  MSEKFSPTLRLGDLNDFIAPSQACVISLKDSKPIVKKSDRPQVVIAPKQ-QLEPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 64

[Close]

BLAST of Carg25117 vs. Swiss-Prot
Match: sp|Q94CL6|NAR1_ARATH (Protein NAR1 OS=Arabidopsis thaliana OX=3702 GN=NAR1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.1e-15
Identity = 43/65 (66.15%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFS TLR+GDLND+IAPSQAC++SLK  K    K D+ +V  + KQ + EPVKISLK
Sbjct: 1  MSEKFSPTLRLGDLNDFIAPSQACVISLKDSKPIVKKSDRPQVVIAPKQ-QLEPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 64

[Close]

BLAST of Carg25117 vs. Swiss-Prot
Match: sp|B4GXC8|NARF_DROPE (Probable cytosolic Fe-S cluster assembly factor GL21135 OS=Drosophila persimilis OX=7234 GN=GL21135 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 30/71 (42.25%), Postives = 40/71 (56.34%), Query Frame = 0


Query: 6  KFSATLRIGDLNDYIAPSQACI---------VSLKGLKATSTKPDKVEVSASRKQLKAEP 65
          +FS  L++ DL+D+I PSQ CI          S  G K T  +    E S S KQ K + 
Sbjct: 3  RFSGALQLTDLDDFITPSQECIKPVTVDKTATSKTGAKITVQEDGYYEESESGKQ-KLQK 62

Query: 66 VKISLKDCLAC 68
          V+I+L+DCLAC
Sbjct: 63 VEITLQDCLAC 72

[Close]

BLAST of Carg25117 vs. Swiss-Prot
Match: sp|B5DK31|NARF_DROPS (Probable cytosolic Fe-S cluster assembly factor GA29080 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA29080 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 30/71 (42.25%), Postives = 40/71 (56.34%), Query Frame = 0


Query: 6  KFSATLRIGDLNDYIAPSQACI---------VSLKGLKATSTKPDKVEVSASRKQLKAEP 65
          +FS  L++ DL+D+I PSQ CI          S  G K T  +    E S S KQ K + 
Sbjct: 3  RFSGALQLTDLDDFITPSQECIKPVTVDKTATSKTGAKITVQEDGYYEESESGKQ-KLQK 62

Query: 66 VKISLKDCLAC 68
          V+I+L+DCLAC
Sbjct: 63 VEITLQDCLAC 72

[Close]

BLAST of Carg25117 vs. Swiss-Prot
Match: sp|A4FV58|NARFL_BOVIN (Cytosolic Fe-S cluster assembly factor NARFL OS=Bos taurus OX=9913 GN=NARFL PE=2 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 1.7e-04
Identity = 29/74 (39.19%), Postives = 39/74 (52.70%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKV---------EVSASRKQLK 62
          M+  FS  L++ DL+D+IAPSQ CI  +K  +   +   K+         +VS      K
Sbjct: 1  MASPFSGALQLTDLDDFIAPSQDCIKPMKVDRRPGSGVAKIHIEDDGSYFQVSQDGGMKK 60

Query: 63 AEPVKISLKDCLAC 68
           E  KISL DCLAC
Sbjct: 61 LEKAKISLDDCLAC 74

[Close]

BLAST of Carg25117 vs. Swiss-Prot
Match: sp|Q7TMW6|NARFL_MOUSE (Cytosolic Fe-S cluster assembly factor NARFL OS=Mus musculus OX=10090 GN=Narfl PE=1 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 1.7e-04
Identity = 27/74 (36.49%), Postives = 39/74 (52.70%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKV---------EVSASRKQLK 62
          M+  FS  L++ DL+D+I PSQ CI  +K  K   +   K+         +V+   +  K
Sbjct: 1  MASPFSGALQLTDLDDFIGPSQNCIKPVKVAKKPGSGIAKIHIEDDGSYFQVNQDGRTQK 60

Query: 63 AEPVKISLKDCLAC 68
           E  K+SL DCLAC
Sbjct: 61 LEKAKVSLNDCLAC 74

[Close]

BLAST of Carg25117 vs. TrEMBL
Match: tr|A0A0A0LVS1|A0A0A0LVS1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G181420 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-25
Identity = 63/66 (95.45%), Postives = 65/66 (98.48%), Query Frame = 0


Query: 2   EMSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISL 61
           EMSEKFSATLRIGDLND+IAPSQACIVSLKGLKAT+TKPDKVEVSASR QLKAEPVKISL
Sbjct: 134 EMSEKFSATLRIGDLNDFIAPSQACIVSLKGLKATATKPDKVEVSASRMQLKAEPVKISL 193

Query: 62  KDCLAC 68
           KDCLAC
Sbjct: 194 KDCLAC 199

[Close]

BLAST of Carg25117 vs. TrEMBL
Match: tr|A0A2P4ITF7|A0A2P4ITF7_QUESU (Protein nar1 OS=Quercus suber OX=58331 GN=CFP56_31867 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 8.6e-22
Identity = 56/65 (86.15%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFSATLRIGDL+D+IAPSQACIVSLKGLKA  TKPDK EVS S+KQL++EPVKISLK
Sbjct: 1  MSEKFSATLRIGDLSDFIAPSQACIVSLKGLKANPTKPDKPEVSISKKQLQSEPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 65

[Close]

BLAST of Carg25117 vs. TrEMBL
Match: tr|A0A2I4EWU4|A0A2I4EWU4_9ROSI (protein NAR1-like OS=Juglans regia OX=51240 GN=LOC108993411 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-21
Identity = 55/65 (84.62%), Postives = 60/65 (92.31%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPDKVEVSASRKQLKAEPVKISLK 62
          MSEKFSATLRIGDL+D+IAPSQACIVSLKGLK+ STKPDK EVS S KQL+ +PVKISLK
Sbjct: 1  MSEKFSATLRIGDLSDFIAPSQACIVSLKGLKSNSTKPDKAEVSTSNKQLQTDPVKISLK 60

Query: 63 DCLAC 68
          DCLAC
Sbjct: 61 DCLAC 65

[Close]

BLAST of Carg25117 vs. TrEMBL
Match: tr|M5WJ36|M5WJ36_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_4G007100 PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 4.3e-21
Identity = 58/66 (87.88%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPD-KVEVSASRKQLKAEPVKISL 62
          MSEKFSATLRIGDLND+IAPSQACIVSLKGLKA+STKP  K EVS S KQLK EPVKISL
Sbjct: 1  MSEKFSATLRIGDLNDFIAPSQACIVSLKGLKASSTKPSAKPEVSTSSKQLKTEPVKISL 60

Query: 63 KDCLAC 68
          KDCLAC
Sbjct: 61 KDCLAC 66

[Close]

BLAST of Carg25117 vs. TrEMBL
Match: tr|A0A140B4A7|A0A140B4A7_PRUPE ([FeFe]-hydrogenase-like protein 36 OS=Prunus persica var. persica OX=323852 GN=NAR1 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 4.3e-21
Identity = 58/66 (87.88%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 3  MSEKFSATLRIGDLNDYIAPSQACIVSLKGLKATSTKPD-KVEVSASRKQLKAEPVKISL 62
          MSEKFSATLRIGDLND+IAPSQACIVSLKGLKA+STKP  K EVS S KQLK EPVKISL
Sbjct: 1  MSEKFSATLRIGDLNDFIAPSQACIVSLKGLKASSTKPSAKPEVSTSSKQLKTEPVKISL 60

Query: 63 KDCLAC 68
          KDCLAC
Sbjct: 61 KDCLAC 66

The following BLAST results are available for this feature:

BLAST of Carg25117 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023001117.1	3.0e-26	100.00	protein NAR1-like [Cucurbita maxima]	[more]
XP_022927572.1	8.7e-26	98.46	protein NAR1-like [Cucurbita moschata] >XP_022927574.1 protein NAR1-like [Cucurb...	[more]
XP_023520381.1	1.5e-25	98.46	protein NAR1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022142658.1	1.9e-25	96.92	protein NAR1 [Momordica charantia]	[more]
KGN65029.1	2.5e-25	95.45	hypothetical protein Csa_1G181420 [Cucumis sativus]	[more]

BLAST of Carg25117 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16440.1	1.2e-16	66.15	ferredoxin hydrogenases	[more]

BLAST of Carg25117 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q94CL6\|NAR1_ARATH	2.1e-15	66.15	Protein NAR1 OS=Arabidopsis thaliana OX=3702 GN=NAR1 PE=1 SV=1	[more]
sp\|B4GXC8\|NARF_DROPE	1.3e-04	42.25	Probable cytosolic Fe-S cluster assembly factor GL21135 OS=Drosophila persimilis...	[more]
sp\|B5DK31\|NARF_DROPS	1.3e-04	42.25	Probable cytosolic Fe-S cluster assembly factor GA29080 OS=Drosophila pseudoobsc...	[more]
sp\|A4FV58\|NARFL_BOVIN	1.7e-04	39.19	Cytosolic Fe-S cluster assembly factor NARFL OS=Bos taurus OX=9913 GN=NARFL PE=2...	[more]
sp\|Q7TMW6\|NARFL_MOUSE	1.7e-04	36.49	Cytosolic Fe-S cluster assembly factor NARFL OS=Mus musculus OX=10090 GN=Narfl P...	[more]

BLAST of Carg25117 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LVS1\|A0A0A0LVS1_CUCSA	1.7e-25	95.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G181420 PE=3 SV=1	[more]
tr\|A0A2P4ITF7\|A0A2P4ITF7_QUESU	8.6e-22	86.15	Protein nar1 OS=Quercus suber OX=58331 GN=CFP56_31867 PE=3 SV=1	[more]
tr\|A0A2I4EWU4\|A0A2I4EWU4_9ROSI	1.1e-21	84.62	protein NAR1-like OS=Juglans regia OX=51240 GN=LOC108993411 PE=3 SV=1	[more]
tr\|M5WJ36\|M5WJ36_PRUPE	4.3e-21	87.88	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_4G007100 PE=3 SV=1	[more]
tr\|A0A140B4A7\|A0A140B4A7_PRUPE	4.3e-21	87.88	[FeFe]-hydrogenase-like protein 36 OS=Prunus persica var. persica OX=323852 GN=N...	[more]

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25117-RA	Carg25117-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11615:SF6	CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR CG17683-RELATED	coord: 7..67
None	No IPR available	PANTHER	PTHR11615	NITRATE, FROMATE, IRON DEHYDROGENASE	coord: 7..67

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg25117	CmoCh18G005870	Cucurbita moschata (Rifu)	carcmoB1391

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg25117	Silver-seed gourd	carcarB055
Carg25117	Cucumber (Gy14) v2	carcgybB1020
Carg25117	Cucumber (Gy14) v2	carcgybB1021
Carg25117	Cucumber (Gy14) v1	carcgyB1154
Carg25117	Cucurbita maxima (Rimu)	carcmaB1435
Carg25117	Cucurbita maxima (Rimu)	carcmaB1436
Carg25117	Cucurbita moschata (Rifu)	carcmoB1390
Carg25117	Cucurbita pepo (Zucchini)	carcpeB1443
Carg25117	Cucurbita pepo (Zucchini)	carcpeB1444
Carg25117	Wild cucumber (PI 183967)	carcpiB1073
Carg25117	Wild cucumber (PI 183967)	carcpiB1074
Carg25117	Cucumber (Chinese Long) v2	carcuB1051
Carg25117	Cucumber (Chinese Long) v2	carcuB1052
Carg25117	Cucumber (Chinese Long) v3	carcucB1083
Carg25117	Cucumber (Chinese Long) v3	carcucB1084
Carg25117	Cucumber (Chinese Long) v3	carcucB1085
Carg25117	Bottle gourd (USVL1VR-Ls)	carlsiB947
Carg25117	Melon (DHL92) v3.5.1	carmeB1006
Carg25117	Melon (DHL92) v3.6.1	carmedB1017
Carg25117	Watermelon (Charleston Gray)	carwcgB1011
Carg25117	Watermelon (Charleston Gray)	carwcgB1012
Carg25117	Watermelon (97103) v1	carwmB1039
Carg25117	Watermelon (97103) v1	carwmB1040
Carg25117	Watermelon (97103) v2	carwmbB1097
Carg25117	Watermelon (97103) v2	carwmbB1098
Carg25117	Wax gourd	carwgoB1216
Carg25117	Wax gourd	carwgoB1217
Carg25117	Wax gourd	carwgoB1218