Carg25039 (gene) Silver-seed gourd

NameCarg25039
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionSerine--tRNA ligase
LocationCucurbita_argyrosperma_scaffold_176 : 362466 .. 366867 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAGGTTTAGGGTTTTTGCAGGTTAAAGCGTTTTCTCTGTCTCTCCCTCTCTCAAGATTGCAGTTTTTGGAATTTATTTGTCGTGCTGTGGGAGGGGGAATCTCAGAGGCGGATAGTGAGAGATGTTGGACATCAATTTGTTCAGGGAAGAGAAGGGCAACAATCCTGAGATCATTCGTGAGTCTCAACGCCGCCGATTTGCCAGCGTTGAACTCGTTGATGAAGTAATTAGGCTTGACAAGGAATGGCGTCAACGTAATCCCCTCAATCTTTTCCATCGCTTTCATTTCTACCGTTATTTGTTACTTTTTTCTTATTTTCTGTTTGGAATTCGATTTTGTTTAGGCCAATTTGAGCTTGAGAATCTCAGGAAGGAGTTCAATAAGATCAACAAGCAAATTGCTCAGCTCAGAATAGTAACGCATCTATCTTTTTTCTTCAAAGCTATTTGTGCTTTCTTGTTTGCGGGTTTAGATTCCACATCTTATCTTCTCTATATTGGTTGCAAGACAAATTAGTTTTGGAACTAGCATTAATTTGAAAGTCTAGGACATTGTCAGTTTCCGATTTGGGGATTTAATACGTTCCATTATTTACTTTGAAAATTTTGGGAATGAAGGCTGGTGAGGATGCGTCGGAGAAGATTAAAGACACTGAAGAAAACAAAAAGTTGTCTGCAGAGAAAGACGTTGAAGCTAAGGATGTGTTAAAACAGTTGAATTCAAAATTGGAAATAATTGGAAACCTTGTGCACGATTCCGTTCCCGTCAGCAATGATGAGGTGTGGTATTTGGGATATCTGCCTTTGTTCTATTCTTAGTAGCGCCATCCTTTTTTTTTTTATTTAAATTTTTAATATTGTACTTAGCGACATTTTGTAGGCTAATAATGCTGTAATCAGATCGTGGGGTGAGAAACTGGTAAAACCAAAACTAAAGAATCATGTTGATCTTGTTGAGCTTCTTGGAATTGCAGATAGCAAAAAAGGTAGTTTGATTTACAGGCTTCTATGGATACAAGATTCATCTTTTATGTCTTCTATTAATTTTGCTGTGGCATTAGGTGTTTCGATCTTATGGTCTTGCTTCTAATCTGTGTTAACTGAAATCTCTTTGTTTTGACTAGATGCTGTATTTGATATCCTTATATCTACAGGAAAAAAAAAAATGATAAAATGAAGAGTAACAAGAATCATATGCTATCTGCACTATAAATGTTTCAAGTTAACCATGTAGAAGGAGATATTACGGCTGCAGATTATTAGATTAGAGCCTTTTTAAGGACATTATTGAATCAAACGGAATAACCACACTTTCAATGAAATTTATCTCTTTTGATAGTTTCACAGATACGGTTCTTATACTTGCTCTAAGTTGGTTTAAAGTTTACTCTGGTTACAAATTACAGATTGAATAGACAGAATTTATCTTTAGATTTTATGTAGCTTTTTTCAGCTCTGGTTGTTCACTTTTATGCTAATTCCAACTTGATGGAAATATGTAGGTTCTGAAATAGCAGGTGGTAGAGGTTACTACCTCAAAGGAGATGGTGTGCGTTTGAACCAAGCTTTGATCAATTATGGCCTCGACTTTTTAGAGAAAAGGGGTTACACAGCAATGCAGACTCCTTTCTTCATGAGAAAAGATATCATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTTTATAAAGTCTGTTATTACCCTCATCTTGTTTCAATGGGATTTTAAGGAATTTTAAATTCTAATGAATTTGAAATGAATCCTTTATTTCTTGAAATTAAATCTTGATTTAAAATCCTGTTTTTCAAGAATTTTAACACGAAGGATCTTACAGGATTTCAATGTCAATTGCCAATAATGAATTTTCTTTTTCATTTCCAAACGAATTCAAATTCATTTCAAATGATTCTCATATTAAACCACACAACAATTTTTAAAATCATTTCTAGTTTCTCATATGATGTGATATAAAATCTTTTCTTATATGTTTTTTTTATTCTAAACATAACCATACTGATTTCCTTCTTTGGAAGTACAGTTTTTAACTGTATTTCAGAACAAATTAGCAGTACAGTTTTTTGCTTTGTTGGCCTGTGGAAACGTGACAAAATGTGACTGAATTAATTGATGTTAATTAGGTTACTGGTGAGGGGGATGATAAGTATCTAATTGCCACTGCTGAACAACCAATTTGTTCATATCATCTTGAAGATTGGATTCATCCTTCCCAATTGCCTATAAGGTTCTTACGTCTCATTGAAAGCCTGTCCACCCTCTGACACCGTAAGAAAGCATTTTTTAAAAAAATAAAATTTACCATTGTTTATGATCTACACAGATATGCTGGGTATTCAACTTGCTTCCGCAAAGAAGCGGGTTCCCATGGTCGAGATACTCTTGGAATTTTCCGAGTCCACCAATTCGAGAAAATAGAGCAATTTTGTGTCACCAGCCCAAATGGCAATGATTCGTGGGAAATGCATGAAGAGATGATTAAGAATTCTGAGGATTTCTACAAGACGGTATCTAACTTCTATTCCTTATGTTAGTGATTGCTTTTTTACATAATTACAATTTTCATGTTCGCAAGCTGGTTTATTTGGCTATTTTGTGAAACATTTGCTTAAACGAGAAATATTGTTATTTTTAGAGGGGTCGAGAGATCTTAGGAAGAGGTTAGTTATATTCTGTCTGATGTGAAATCCAATGTTTTGGCAGTTAAATCTCCCATACCAAGTTGTGGCTATAGTTTCTGGTGCATTAAATGATGCGGCTGCCAAGAAATACGATCTGGAAGCATGGTTTCCTGCATCCCAGACTTACAGGGAGTTAGTATCCTGTTCAAATTGCACTGATTATCAGTCCAGAAGATTAGAGACCCGATTTGGTCAGAAAAAGGTTAGTTCATTTCTTACTTCTGAATTATTGGAGGATTTATTTCTGTCATTAAAATTAAACCCGGTGGTACTAACGTTTTAATAGGCTTCTAATAGGGAGGAATTGATTCTTATAGCTAATTACGTCTGTGACTATGATGCTTTTTGTTCGGGTTTTTTTTAATGCTTTTGTACATTCTTTCATTTCCCCCTTTTTACAAAGTATATGATTCTTGCATTCTTGTAAGTTTAGTATTGAATTTTCAGGTCTTGTGTCCATTCTTTGTGTTTAGTCAAGTTTTTTATTGTTTTAAAAAGTTCGTAGGAGTTTAAATATTTGACTTGTCTCTTTTAGTCCTTATATTTTCAAAAGATTCTGATATGCCCTTTGAGTTTTTAATTCTGTATTTAATCTCTATTTATAATTCTATACTTTAATGCCTATGCCACACCGTATCTGATCTGATTTAAGTTTTAATGCTTAGGCCACACAATATCTAAACCAGCCTGAAATCAACTCAGCACTACACACTTAGATATTACAGTCAACGTGCCTCATATGCACGAAATTGACTGTTTTAACATCAGTGACCAACCTACTACTGATCCATTAGACACAAAATGAGAGTAACTTGGTAGAGAAAACTATTAGAAACCTTTTCAATTACAAGAATTAGATAGATACAACCTTGAAAGGTTAGGGATTAACTATGAACATTGTAAAAGCACATAGACGATAGACAACATTGAGAAGTTTAAAAGTATACAAACAACATGGATGCAATCTTACGAGTGCAAGGAAGAAACCAATAATCTTTCTTTTCCGTTCCAATATTTGCAGAGCAACGAGCAGACAAAGCAATATGTGCACATGCTGAATTCCACGCTCACAGCAACAGAAAGGACTTTGTGCTGCATTCTTGAGAACTACCAGAAAGAAGATGGAGTGGAGATACCAGAAGTTCTACAACCATTTATGGGTGGAAAAACTTTTATGCCCTTCAAGGCGAAACCAACAACAAGCGATGCCAAAGGGAAGAAATCGAAGGCCTGATTGCATAATTTTCTTCTCTCCCATTTGATTTATAAAATTTCTGACTCAAGGAAAGGATATATTACTCACAGTATTGTTGGAACTTCAAATTATCTTTTTGGTGGGCATGGAGTTATATTACACTAATAACAGATGGCTGTTCTTATTTATATGCTTCAATTGTGGGTACTCTCTCTATAAAAGAGTTGTAGATTGAATTTTCTGATTTAGAGAAGCTTGGCAATCGTTTCTGTCGACTTAGGTTCTTAATTGATTTTATTTAGACTGAAACAACAAGTATTTGGAAGCTCATTTGAGAGAGAATATGTTCGGATTCTAAACTAATTTTCTTGGGCTTTTGCAGGGCTGCCAATCGTTTTCATCAACCCTGTCAAGCAAGCCCAAAGCTGAGTCCAGTAATGAAATTCATGGACCACTCCCACATGGTCTGGAAACTCTTATTTAGAAAATTCAGATTATTAGG

mRNA sequence

CTCAGGTTTAGGGTTTTTGCAGGTTAAAGCGTTTTCTCTGTCTCTCCCTCTCTCAAGATTGCAGTTTTTGGAATTTATTTGTCGTGCTGTGGGAGGGGGAATCTCAGAGGCGGATAGTGAGAGATGTTGGACATCAATTTGTTCAGGGAAGAGAAGGGCAACAATCCTGAGATCATTCGTGAGTCTCAACGCCGCCGATTTGCCAGCGTTGAACTCGTTGATGAAGTAATTAGGCTTGACAAGGAATGGCGTCAACGCCAATTTGAGCTTGAGAATCTCAGGAAGGAGTTCAATAAGATCAACAAGCAAATTGCTCAGCTCAGAATAGCTGGTGAGGATGCGTCGGAGAAGATTAAAGACACTGAAGAAAACAAAAAGTTGTCTGCAGAGAAAGACGTTGAAGCTAAGGATGTGTTAAAACAGTTGAATTCAAAATTGGAAATAATTGGAAACCTTGTGCACGATTCCGTTCCCGTCAGCAATGATGAGGCTAATAATGCTGTAATCAGATCGTGGGGTGAGAAACTGGTAAAACCAAAACTAAAGAATCATGTTGATCTTGTTGAGCTTCTTGGAATTGCAGATAGCAAAAAAGGTTCTGAAATAGCAGGTGGTAGAGGTTACTACCTCAAAGGAGATGGTGTGCGTTTGAACCAAGCTTTGATCAATTATGGCCTCGACTTTTTAGAGAAAAGGGGTTACACAGCAATGCAGACTCCTTTCTTCATGAGAAAAGATATCATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTTTATAAAGTTACTGGTGAGGGGGATGATAAGTATCTAATTGCCACTGCTGAACAACCAATTTGTTCATATCATCTTGAAGATTGGATTCATCCTTCCCAATTGCCTATAAGATATGCTGGGTATTCAACTTGCTTCCGCAAAGAAGCGGGTTCCCATGGTCGAGATACTCTTGGAATTTTCCGAGTCCACCAATTCGAGAAAATAGAGCAATTTTGTGTCACCAGCCCAAATGGCAATGATTCGTGGGAAATGCATGAAGAGATGATTAAGAATTCTGAGGATTTCTACAAGACGTTAAATCTCCCATACCAAGTTGTGGCTATAGTTTCTGGTGCATTAAATGATGCGGCTGCCAAGAAATACGATCTGGAAGCATGGTTTCCTGCATCCCAGACTTACAGGGAGTTAGTATCCTGTTCAAATTGCACTGATTATCAGTCCAGAAGATTAGAGACCCGATTTGGTCAGAAAAAGAGCAACGAGCAGACAAAGCAATATGTGCACATGCTGAATTCCACGCTCACAGCAACAGAAAGGACTTTGTGCTGCATTCTTGAGAACTACCAGAAAGAAGATGGAGTGGAGATACCAGAAGTTCTACAACCATTTATGGGTGGAAAAACTTTTATGCCCTTCAAGGCGAAACCAACAACAAGCGATGCCAAAGGGAAGAAATCGAAGGCCTGATTGCATAATTTTCTTCTCTCCCATTTGATTTATAAAATTTCTGACTCAAGGAAAGGATATATTACTCACAGTATTGTTGGAACTTCAAATTATCTTTTTGGTGGGCATGGAGTTATATTACACTAATAACAGATGGCTGTTCTTATTTATATGCTTCAATTGTGGGTACTCTCTCTATAAAAGAGTTGTAGATTGAATTTTCTGATTTAGAGAAGCTTGGCAATCGTTTCTGTCGACTTAGGTTCTTAATTGATTTTATTTAGACTGAAACAACAAGTATTTGGAAGCTCATTTGAGAGAGAATATGTTCGGATTCTAAACTAATTTTCTTGGGCTTTTGCAGGGCTGCCAATCGTTTTCATCAACCCTGTCAAGCAAGCCCAAAGCTGAGTCCAGTAATGAAATTCATGGACCACTCCCACATGGTCTGGAAACTCTTATTTAGAAAATTCAGATTATTAGG

Coding sequence (CDS)

ATGTTGGACATCAATTTGTTCAGGGAAGAGAAGGGCAACAATCCTGAGATCATTCGTGAGTCTCAACGCCGCCGATTTGCCAGCGTTGAACTCGTTGATGAAGTAATTAGGCTTGACAAGGAATGGCGTCAACGCCAATTTGAGCTTGAGAATCTCAGGAAGGAGTTCAATAAGATCAACAAGCAAATTGCTCAGCTCAGAATAGCTGGTGAGGATGCGTCGGAGAAGATTAAAGACACTGAAGAAAACAAAAAGTTGTCTGCAGAGAAAGACGTTGAAGCTAAGGATGTGTTAAAACAGTTGAATTCAAAATTGGAAATAATTGGAAACCTTGTGCACGATTCCGTTCCCGTCAGCAATGATGAGGCTAATAATGCTGTAATCAGATCGTGGGGTGAGAAACTGGTAAAACCAAAACTAAAGAATCATGTTGATCTTGTTGAGCTTCTTGGAATTGCAGATAGCAAAAAAGGTTCTGAAATAGCAGGTGGTAGAGGTTACTACCTCAAAGGAGATGGTGTGCGTTTGAACCAAGCTTTGATCAATTATGGCCTCGACTTTTTAGAGAAAAGGGGTTACACAGCAATGCAGACTCCTTTCTTCATGAGAAAAGATATCATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTTTATAAAGTTACTGGTGAGGGGGATGATAAGTATCTAATTGCCACTGCTGAACAACCAATTTGTTCATATCATCTTGAAGATTGGATTCATCCTTCCCAATTGCCTATAAGATATGCTGGGTATTCAACTTGCTTCCGCAAAGAAGCGGGTTCCCATGGTCGAGATACTCTTGGAATTTTCCGAGTCCACCAATTCGAGAAAATAGAGCAATTTTGTGTCACCAGCCCAAATGGCAATGATTCGTGGGAAATGCATGAAGAGATGATTAAGAATTCTGAGGATTTCTACAAGACGTTAAATCTCCCATACCAAGTTGTGGCTATAGTTTCTGGTGCATTAAATGATGCGGCTGCCAAGAAATACGATCTGGAAGCATGGTTTCCTGCATCCCAGACTTACAGGGAGTTAGTATCCTGTTCAAATTGCACTGATTATCAGTCCAGAAGATTAGAGACCCGATTTGGTCAGAAAAAGAGCAACGAGCAGACAAAGCAATATGTGCACATGCTGAATTCCACGCTCACAGCAACAGAAAGGACTTTGTGCTGCATTCTTGAGAACTACCAGAAAGAAGATGGAGTGGAGATACCAGAAGTTCTACAACCATTTATGGGTGGAAAAACTTTTATGCCCTTCAAGGCGAAACCAACAACAAGCGATGCCAAAGGGAAGAAATCGAAGGCCTGA

Protein sequence

MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKINKQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSNDEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQALINYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPICSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGNDSWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVLQPFMGGKTFMPFKAKPTTSDAKGKKSKA
BLAST of Carg25039 vs. NCBI nr
Match: XP_022926250.1 (serine--tRNA ligase [Cucurbita moschata])

HSP 1 Score: 896.0 bits (2314), Expect = 5.3e-257
Identity = 447/448 (99.78%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           QPFMGGKTFMPFKAKPTTSDAKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of Carg25039 vs. NCBI nr
Match: XP_023530248.1 (serine--tRNA ligase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 894.8 bits (2311), Expect = 1.2e-256
Identity = 446/448 (99.55%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           QPFMGGKTFMPFKAKPTTSDAKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of Carg25039 vs. NCBI nr
Match: XP_022969645.1 (serine--tRNA ligase [Cucurbita maxima])

HSP 1 Score: 892.9 bits (2306), Expect = 4.5e-256
Identity = 445/448 (99.33%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPAS TYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASHTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           QPFMGGKTFMPFKAKPTTSDAKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of Carg25039 vs. NCBI nr
Match: XP_022145958.1 (serine--tRNA ligase [Momordica charantia] >XP_022145959.1 serine--tRNA ligase [Momordica charantia])

HSP 1 Score: 875.5 bits (2261), Expect = 7.5e-251
Identity = 430/448 (95.98%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANNA+IRSWGEKLVKPKLKNHVDLVELLGIAD+KKGSEIAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           INYGLDFLEKRGYTA+QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           C+YHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           QPFMGGKTF+PFKAKP+TS+AKGKKSKA
Sbjct: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448

BLAST of Carg25039 vs. NCBI nr
Match: XP_008464736.1 (PREDICTED: serine--tRNA ligase, cytoplasmic [Cucumis melo] >XP_008464737.1 PREDICTED: serine--tRNA ligase, cytoplasmic [Cucumis melo])

HSP 1 Score: 854.4 bits (2206), Expect = 1.8e-244
Identity = 416/448 (92.86%), Postives = 440/448 (98.21%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASE+IKDTEENK+LSAEKDVEAKDVLK+LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANN VIRSWGEKLVKPKLKNHVDLVELL IAD+K+GSEIAGGRGYYLKG GVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           INYGLDFLEKRGYT +QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYHLEDWIHPSQLP+RYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVE+PEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           +P++GGK F+PFK KPT +D+KGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of Carg25039 vs. TAIR10
Match: AT5G27470.1 (seryl-tRNA synthetase / serine--tRNA ligase)

HSP 1 Score: 740.0 bits (1909), Expect = 8.9e-214
Identity = 353/451 (78.27%), Postives = 409/451 (90.69%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFASVE+VDE+I+LDKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQ+AQL+I  EDASE I+ TE+NK+ S  K+ E ++    L +KLE +GNLVHDSVPV  
Sbjct: 61  KQVAQLKIKKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDK 120

Query: 121 DEANNAVIRSWGEKLVKP---KLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLN 180
           DEANN VI+ WGEK       KLKNHVDLVELLGIAD+K+G+EIAG RG++LKGDG+ LN
Sbjct: 121 DEANNLVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLN 180

Query: 181 QALINYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAE 240
           QALIN+GL FL+KRG+T +Q PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAE
Sbjct: 181 QALINFGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAE 240

Query: 241 QPICSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPN 300
           QP+C+YH+++WIHP++LP+RYAGYS+CFRKEAGSHGRDTLGIFRVHQFEKIEQFC+T PN
Sbjct: 241 QPLCAYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPN 300

Query: 301 GNDSWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELV 360
            N SWEM +EM+KNSEDFY+ L LPYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELV
Sbjct: 301 ENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELV 360

Query: 361 SCSNCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIP 420
           SCSNCTDYQ+RRLE R+GQKKSNEQTKQYVHMLNSTLTATERT+CCILENYQ+EDGV+IP
Sbjct: 361 SCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIP 420

Query: 421 EVLQPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           EVLQPFMGG+TF+PFKAKP  +D KGKKSKA
Sbjct: 421 EVLQPFMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of Carg25039 vs. TAIR10
Match: AT1G11870.2 (Seryl-tRNA synthetase)

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 141/426 (33.10%), Postives = 227/426 (53.29%), Query Frame = 0

Query: 13  NNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKINKQIAQLRIAGED 72
           +N E +  + R R ++  L + V++L +     Q E+E LR+E N + K++ + ++   +
Sbjct: 86  DNKEAVEINIRNRNSNANL-EAVLQLYENMVNLQKEVERLREERNNVAKKM-KGKLEPSE 145

Query: 73  ASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSNDEANNAVIRSWG 132
               ++                             I N+ H  VPV  ++++        
Sbjct: 146 RERLVEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIPNMTHPDVPVGGEDSSAIRQEVGS 205

Query: 133 EKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQALINYGLDFLEKRG 192
            +     +K+H+ L + L + D    +E++G + +YLK + V L  AL+N+ L  + K+G
Sbjct: 206 PREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALLNWTLSQVMKKG 265

Query: 193 YTAMQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQPICSYHLEDWIHP 252
           YT + TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE P+   H++  +  
Sbjct: 266 YTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPVGGIHMDSILLE 325

Query: 253 SQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGNDSWEMHEEMIKN 312
           S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F +  P   DS   H+E+I+ 
Sbjct: 326 SALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQP--EDSESFHQELIQI 385

Query: 313 SEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQSRRLE 372
            ED + +L L ++ + + +  L   A +K+D+EAW P    + E+ S SNCTDYQSRRL 
Sbjct: 386 EEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSRRLG 445

Query: 373 TRF--------GQK--KSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDG-VEIPEVL 427
            R+        G K  K+N    ++VH LN+T  A  R + C+LENYQ+EDG V IPE L
Sbjct: 446 IRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGSVVIPEPL 505

BLAST of Carg25039 vs. Swiss-Prot
Match: sp|Q39230|SYSC_ARATH (Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g27470 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 1.6e-212
Identity = 353/451 (78.27%), Postives = 409/451 (90.69%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFASVE+VDE+I+LDKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQ+AQL+I  EDASE I+ TE+NK+ S  K+ E ++    L +KLE +GNLVHDSVPV  
Sbjct: 61  KQVAQLKIKKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDK 120

Query: 121 DEANNAVIRSWGEKLVKP---KLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLN 180
           DEANN VI+ WGEK       KLKNHVDLVELLGIAD+K+G+EIAG RG++LKGDG+ LN
Sbjct: 121 DEANNLVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLN 180

Query: 181 QALINYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAE 240
           QALIN+GL FL+KRG+T +Q PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAE
Sbjct: 181 QALINFGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAE 240

Query: 241 QPICSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPN 300
           QP+C+YH+++WIHP++LP+RYAGYS+CFRKEAGSHGRDTLGIFRVHQFEKIEQFC+T PN
Sbjct: 241 QPLCAYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPN 300

Query: 301 GNDSWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELV 360
            N SWEM +EM+KNSEDFY+ L LPYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELV
Sbjct: 301 ENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELV 360

Query: 361 SCSNCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIP 420
           SCSNCTDYQ+RRLE R+GQKKSNEQTKQYVHMLNSTLTATERT+CCILENYQ+EDGV+IP
Sbjct: 361 SCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIP 420

Query: 421 EVLQPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           EVLQPFMGG+TF+PFKAKP  +D KGKKSKA
Sbjct: 421 EVLQPFMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of Carg25039 vs. Swiss-Prot
Match: sp|O81983|SYS_HELAN (Serine--tRNA ligase OS=Helianthus annuus OX=4232 PE=2 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 2.0e-210
Identity = 351/435 (80.69%), Postives = 396/435 (91.03%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKG NPEI+RESQRRR ASVE+VDE+I+LDKEWRQRQFELE LRK+FN+IN
Sbjct: 1   MLDINLFREEKGGNPEIVRESQRRRGASVEIVDEIIKLDKEWRQRQFELEQLRKDFNRIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           K++A+LRI+G DAS  IK+TEENK  +A+K  E ++    L SKLE +GNLVHDSVPVSN
Sbjct: 61  KEVAKLRISGGDASSLIKNTEENKDSTAKKQAEVQEARTALYSKLETVGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEA+NAV+R+WGE+  +  LKNHV+LVELLGIAD KKG+ +AGGRGYYLKGDGVRLNQAL
Sbjct: 121 DEADNAVVRTWGERREETNLKNHVELVELLGIADLKKGANVAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           IN+GLDFLEKR YTA+QTPFFMRKDIM +CAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INFGLDFLEKRKYTALQTPFFMRKDIMGKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           C+YH++DWIHP++LP+RYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+T P+GND
Sbjct: 241 CAYHIDDWIHPNELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCLTKPSGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWEMHEEMIKNSE+FY+ L LPYQVV+IVSGALNDAAAKKYDLEAWFPAS+TYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEEFYQMLKLPYQVVSIVSGALNDAAAKKYDLEAWFPASKTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSR+LE R GQKK NEQ KQY H+LNSTLTATERTLCCILENYQ E GV IPEVL
Sbjct: 361 NCTDYQSRKLEIRCGQKKGNEQAKQYCHLLNSTLTATERTLCCILENYQTEKGVAIPEVL 420

Query: 421 QPFMGGKTFMPFKAK 436
           QPFMGGKTF+ F  K
Sbjct: 421 QPFMGGKTFLDFLKK 435

BLAST of Carg25039 vs. Swiss-Prot
Match: sp|Q7KWQ2|SYSC_DICDI (Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=serS PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 4.8e-140
Identity = 249/436 (57.11%), Postives = 319/436 (73.17%), Query Frame = 0

Query: 2   LDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKINK 61
           LDI LFR +KG NP++IR+SQ+ R+A+V+ V EVI LDK  ++ ++ L+N   E+ K+NK
Sbjct: 3   LDIVLFRADKGGNPDLIRQSQKVRYANVDAVQEVIDLDKVVKETKYRLDNTNAEYAKLNK 62

Query: 62  QIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSND 121
            +A  + AGE A E I   EE  +   +   E  +V   L  KL  IGN+VH+SVP+ N+
Sbjct: 63  SVAMKKKAGESADEIIAQAEELNQSIIKLKAEVSEVELLLRKKLRPIGNIVHESVPIDNN 122

Query: 122 EANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQALI 181
           E NN +I++WGE      L +H +L+E++   D ++G+ ++G R Y+LKG GV LNQA+I
Sbjct: 123 EDNNQIIKTWGECKTSEGLLHHHELLEMIDGYDPERGTLVSGHRCYFLKGIGVLLNQAII 182

Query: 182 NYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPIC 241
           N+ L  + KRG   +QTPFFM KD+MA+ AQL QFD+ELYKVTG+ ++KYLIAT+EQPI 
Sbjct: 183 NFALMHMTKRGSVPLQTPFFMNKDVMAKTAQLEQFDDELYKVTGDNEEKYLIATSEQPIS 242

Query: 242 SYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGNDS 301
           ++H ++WI    LP +Y GYSTCFRKEAGSHGRDT GIFRVHQFEKIEQFC+T P    S
Sbjct: 243 AFHQDEWIEEKDLPKKYVGYSTCFRKEAGSHGRDTWGIFRVHQFEKIEQFCITEP--EKS 302

Query: 302 WEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSN 361
           W+M EEMI NSE FY+ L +PY+VV IVSGALN+AA+KKYDLE WFP    YRELVSCSN
Sbjct: 303 WDMMEEMINNSEQFYQELGIPYRVVNIVSGALNNAASKKYDLEGWFPGYNQYRELVSCSN 362

Query: 362 CTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVLQ 421
           CTDYQSR LE R G KK  +Q K+YVHMLNSTL AT R +CCILENYQ E G+ +P  L+
Sbjct: 363 CTDYQSRDLEIRCGMKKQGQQQKKYVHMLNSTLAATTRVICCILENYQTEGGITVPVPLR 422

Query: 422 PFMGGKTFMPF-KAKP 437
           P++ GK F+PF KA P
Sbjct: 423 PYL-GKDFIPFVKAAP 435

BLAST of Carg25039 vs. Swiss-Prot
Match: sp|O14018|SYSC_SCHPO (Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29A4.15 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.1e-139
Identity = 264/456 (57.89%), Postives = 329/456 (72.15%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLF+ EKG NPEIIRESQR+R A V +VD+VI + KEW   +FEL+N  K  N++ 
Sbjct: 1   MLDINLFQVEKGGNPEIIRESQRKRGADVGVVDKVIEMYKEWVSLRFELDNTNKSINRVQ 60

Query: 61  KQIAQLRIAGEDASEKIKD----TEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSV 120
           K+I     A EDASE +++    T     L  ++  + K++L  ++S    IGN+VHDSV
Sbjct: 61  KEIGLKMKAKEDASELLEEKNSLTXXXXXLIEQETAKNKEMLNVVSS----IGNIVHDSV 120

Query: 121 PVSNDEANNAVIRSWGEKLVKPKLKN---HVDLVELLGIADSKKGSEIAGGRGYYLKGDG 180
           PVS DE NN +IR W  + V  + KN   H +++  L   D ++G +++G RGY+L+  G
Sbjct: 121 PVSMDEDNNEIIRKWAPEGVTVEKKNCLSHHEVLTRLDGYDPERGVKVSGHRGYFLRQYG 180

Query: 181 VRLNQALINYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLI 240
           V  N ALI YGLDFLEKRGY A+Q P  + KD+MA+ AQL QFDEELYKV    +++YLI
Sbjct: 181 VFFNLALIQYGLDFLEKRGYIALQAPTMLNKDVMAKTAQLEQFDEELYKVIDGDEERYLI 240

Query: 241 ATAEQPICSYHLEDWIH-PS-QLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQF 300
           AT+EQPI +YH  +W   PS QLP++YAGYSTC+R+EAGSHGRD  GIFRVH FEKIEQF
Sbjct: 241 ATSEQPISAYHSGEWFEKPSEQLPLKYAGYSTCYRREAGSHGRDAWGIFRVHAFEKIEQF 300

Query: 301 CVTSPNGNDSWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQ 360
            +T P    SWE   EMI ++EDFYK+L LPY++VAIVSGALN+AAAKKYDLEAWFP   
Sbjct: 301 VLTDP--EKSWEAFTEMINHAEDFYKSLELPYRIVAIVSGALNNAAAKKYDLEAWFPFQG 360

Query: 361 TYRELVSCSNCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKE 420
            Y+ELVSCSNCTDYQSR LE R G KK  ++ K+YVH LNSTL ATER LCCILENYQ  
Sbjct: 361 EYKELVSCSNCTDYQSRNLEIRCGVKKMGDREKKYVHCLNSTLCATERALCCILENYQTP 420

Query: 421 DGVEIPEVLQPFMGGKTFMPF-KAKPTTSDAKGKKS 447
           DGV +P+VLQP+MGGKTF+PF K  P  S +K  K+
Sbjct: 421 DGVNVPKVLQPYMGGKTFLPFTKELPKNSTSKKGKN 450

BLAST of Carg25039 vs. Swiss-Prot
Match: sp|Q9HGT6|SYSC_CANAL (Serine--tRNA ligase, cytoplasmic OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=SES1 PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 9.0e-131
Identity = 258/465 (55.48%), Postives = 322/465 (69.25%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDIN F  EKG +PEII+ SQ++R  SVELVDE+I   KEW + +F+L+   K+ N + 
Sbjct: 1   MLDINAFLVEKGGDPEIIKASQKKRGDSVELVDEIIAEYKEWVKLRFDLDEHNKKLNSVQ 60

Query: 61  KQIAQLRIAGEDASEKIKDTEE---NKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVP 120
           K+I +   A EDA + I + E+    KK   EK+ EA    K L SK+  +GN+VH+SV 
Sbjct: 61  KEIGKRFKAKEDAKDLIAEKEKLSNEKKEIIEKEAEAD---KNLRSKINQVGNIVHESVV 120

Query: 121 VSNDEANNAVIRSW-GEKLVKP-----------KLKNHVDLVELLGIADSKKGSEIAGGR 180
            S DE NN ++R+W  E   KP           KL +H  L+ L G  D ++G  I G R
Sbjct: 121 DSQDEENNELVRTWTPENYKKPEQIAAATGAPAKLSHHEVLLRLDGY-DPERGVRIVGHR 180

Query: 181 GYYLKGDGVRLNQALINYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTG 240
           GY+L+  GV LNQALINYGL FL  +GY  +Q P  M K++MA+ AQL+QFDEELYKV  
Sbjct: 181 GYFLRNYGVFLNQALINYGLSFLSSKGYVPLQAPVMMNKEVMAKTAQLSQFDEELYKVID 240

Query: 241 EGDDKYLIATAEQPICSYHLEDWIH--PSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVH 300
             D+KYLIAT+EQPI +YH  +W      QLP+RYAGYS+CFR+EAGSHG+D  GIFRVH
Sbjct: 241 GEDEKYLIATSEQPISAYHAGEWFESPAEQLPVRYAGYSSCFRREAGSHGKDAWGIFRVH 300

Query: 301 QFEKIEQFCVTSPNGNDSWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDL 360
            FEKIEQF +T P    SWE  + MI  SE+FY++L LPY+VV IVSG LN+AAAKKYDL
Sbjct: 301 AFEKIEQFVLTEP--EKSWEEFDRMIGCSEEFYQSLGLPYRVVGIVSGELNNAAAKKYDL 360

Query: 361 EAWFPASQTYRELVSCSNCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCC 420
           EAWFP  Q Y+ELVSCSNCTDYQSR LE R G K+ N+Q K+YVH LNSTL+ATERT+CC
Sbjct: 361 EAWFPFQQEYKELVSCSNCTDYQSRNLEIRCGIKQQNQQEKKYVHCLNSTLSATERTICC 420

Query: 421 ILENYQKEDGVEIPEVLQPFMGGK-TFMPF---KAKPTTSDAKGK 445
           ILENYQKEDG+ IPEVL+ ++ G+  F+P+     K TTS  K K
Sbjct: 421 ILENYQKEDGLVIPEVLRKYIPGEPEFIPYIKELPKNTTSVKKAK 459

BLAST of Carg25039 vs. TrEMBL
Match: tr|A0A1S3CM54|A0A1S3CM54_CUCME (serine--tRNA ligase, cytoplasmic OS=Cucumis melo OX=3656 GN=LOC103502550 PE=4 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 1.2e-244
Identity = 416/448 (92.86%), Postives = 440/448 (98.21%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASE+IKDTEENK+LSAEKDVEAKDVLK+LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANN VIRSWGEKLVKPKLKNHVDLVELL IAD+K+GSEIAGGRGYYLKG GVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           INYGLDFLEKRGYT +QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYHLEDWIHPSQLP+RYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVE+PEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           +P++GGK F+PFK KPT +D+KGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of Carg25039 vs. TrEMBL
Match: tr|A0A0A0KL58|A0A0A0KL58_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G002590 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 2.0e-244
Identity = 418/448 (93.30%), Postives = 439/448 (97.99%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASE+IKDTEENKKLSAEKDVEAKDVLK+LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANN VIRSWGEKLVKPKLKNHVDLVELL IADSK+GSEIAGGRGYYLKG GVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           INYGLDFLEKRGYT +QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPN ND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSE+FYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVE+PEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           +P++GGK F+PFK KPT SD+KGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 448

BLAST of Carg25039 vs. TrEMBL
Match: tr|M5VY64|M5VY64_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G327000 PE=4 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 1.5e-231
Identity = 391/448 (87.28%), Postives = 431/448 (96.21%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRF +VE+VDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFKAVEIVDEVIQLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           K++AQLRIAGEDA+  IKDTEENK+L+AEK+VE ++ L QLNSKLE+IGNLVHDSVP+SN
Sbjct: 61  KRVAQLRIAGEDATAVIKDTEENKRLAAEKEVEVREALTQLNSKLEVIGNLVHDSVPISN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DE NNAVIRSWGEK ++PKLKNHV+LVELLGIAD KKG+E+AGGRG+YLKG GVRLNQAL
Sbjct: 121 DEENNAVIRSWGEKRMEPKLKNHVELVELLGIADLKKGAEVAGGRGFYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           IN+GLDFLEKRGYT +QTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INFGLDFLEKRGYTELQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYH++DWIHPSQLP+RYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSP+G+D
Sbjct: 241 CSYHIDDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPDGSD 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWEMHEEMIKNSEDFYK+LNLPY VVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEDFYKSLNLPYHVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSR+LETR+GQKKSNEQTK+YVH+LNSTLTATERTLCCILENYQ+EDGV+IPEVL
Sbjct: 361 NCTDYQSRKLETRYGQKKSNEQTKKYVHLLNSTLTATERTLCCILENYQREDGVDIPEVL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           QPFMGGKTF+PFK KP   + KGKKSKA
Sbjct: 421 QPFMGGKTFLPFKNKP-APEVKGKKSKA 447

BLAST of Carg25039 vs. TrEMBL
Match: tr|A0A2P5DSW6|A0A2P5DSW6_PARAD (Serine-tRNA ligase, type OS=Parasponia andersonii OX=3476 GN=PanWU01x14_034380 PE=4 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 1.8e-229
Identity = 387/448 (86.38%), Postives = 428/448 (95.54%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+V+LVDEVI+LDKEWR RQ++LE+LRKEFN+IN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVDLVDEVIKLDKEWRLRQYDLEDLRKEFNRIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           K++A+L+I G+DA+EKIK TE+ KK  AEK+VE ++ L+QLNS+LEIIGNLVHDSVP+SN
Sbjct: 61  KEVARLKIEGKDATEKIKSTEDTKKSIAEKEVEVREALQQLNSRLEIIGNLVHDSVPISN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANNAVIRSWGEK  +PKLKNHV+LVELLGIAD+KKGS++AGGRGYYLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKRTEPKLKNHVELVELLGIADTKKGSDVAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           IN+GLDFLE RGYTA+QTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INFGLDFLENRGYTALQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           CSYHL+DWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLDDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKVEQFCLTSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYK LN+PYQ+VAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKMLNIPYQIVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQ KQYVH+LNSTLTATERTLCCILENYQKEDGVEIPE L
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQAKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEAL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           + FMGGKTF+PFK KP  S+ KGKKS+A
Sbjct: 421 REFMGGKTFIPFKTKP-ASETKGKKSRA 447

BLAST of Carg25039 vs. TrEMBL
Match: tr|D7U1Z6|D7U1Z6_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_14s0128g00370 PE=4 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 3.1e-229
Identity = 387/448 (86.38%), Postives = 427/448 (95.31%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFRE+KG+NPEIIRESQRRR+A+VELVDEVI LDKEWRQRQFE ENLRKEFNKIN
Sbjct: 1   MLDINLFREDKGHNPEIIRESQRRRYANVELVDEVIHLDKEWRQRQFEYENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQ+A+L+I+GEDASE IK T+ENK+L+++K+ E ++ L  LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQVAKLKISGEDASEMIKSTDENKRLTSKKEAEVQEALAALNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180
           DEANNAVIRSWGEK V+PKLKNHV+LVELLGIAD KKG+ IAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKKVEPKLKNHVELVELLGIADLKKGANIAGGRGFYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240
           IN+GLDFLEKRGYTA+QTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INFGLDFLEKRGYTALQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300
           C+YHL+DWIHPSQLP+RYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CAYHLDDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYK LN+PYQVVAIVSGALNDAAAKKYDLE WFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKMLNIPYQVVAIVSGALNDAAAKKYDLEGWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQ KQYVH+LNSTLTATERT+CCILENYQKEDGVE+PE L
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQIKQYVHLLNSTLTATERTICCILENYQKEDGVEVPEPL 420

Query: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 449
           +PFMGGKTF+PF+  P T +AKGKKSKA
Sbjct: 421 RPFMGGKTFLPFQTSPAT-EAKGKKSKA 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022926250.15.3e-25799.78serine--tRNA ligase [Cucurbita moschata][more]
XP_023530248.11.2e-25699.55serine--tRNA ligase [Cucurbita pepo subsp. pepo][more]
XP_022969645.14.5e-25699.33serine--tRNA ligase [Cucurbita maxima][more]
XP_022145958.17.5e-25195.98serine--tRNA ligase [Momordica charantia] >XP_022145959.1 serine--tRNA ligase [M... [more]
XP_008464736.11.8e-24492.86PREDICTED: serine--tRNA ligase, cytoplasmic [Cucumis melo] >XP_008464737.1 PREDI... [more]
Match NameE-valueIdentityDescription
AT5G27470.18.9e-21478.27seryl-tRNA synthetase / serine--tRNA ligase[more]
AT1G11870.21.5e-5933.10Seryl-tRNA synthetase[more]
Match NameE-valueIdentityDescription
sp|Q39230|SYSC_ARATH1.6e-21278.27Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g27470 PE... [more]
sp|O81983|SYS_HELAN2.0e-21080.69Serine--tRNA ligase OS=Helianthus annuus OX=4232 PE=2 SV=1[more]
sp|Q7KWQ2|SYSC_DICDI4.8e-14057.11Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=serS PE... [more]
sp|O14018|SYSC_SCHPO3.1e-13957.89Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
sp|Q9HGT6|SYSC_CANAL9.0e-13155.48Serine--tRNA ligase, cytoplasmic OS=Candida albicans (strain SC5314 / ATCC MYA-2... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CM54|A0A1S3CM54_CUCME1.2e-24492.86serine--tRNA ligase, cytoplasmic OS=Cucumis melo OX=3656 GN=LOC103502550 PE=4 SV... [more]
tr|A0A0A0KL58|A0A0A0KL58_CUCSA2.0e-24493.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G002590 PE=4 SV=1[more]
tr|M5VY64|M5VY64_PRUPE1.5e-23187.28Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G327000 PE=4 SV=1[more]
tr|A0A2P5DSW6|A0A2P5DSW6_PARAD1.8e-22986.38Serine-tRNA ligase, type OS=Parasponia andersonii OX=3476 GN=PanWU01x14_034380 P... [more]
tr|D7U1Z6|D7U1Z6_VITVI3.1e-22986.38Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_14s0128g00370 PE=4 SV=... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004812aminoacyl-tRNA ligase activity
GO:0004828serine-tRNA ligase activity
GO:0005524ATP binding
GO:0000166nucleotide binding
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
GO:0006434seryl-tRNA aminoacylation
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: INTERPRO
TermDefinition
IPR010978tRNA-bd_arm
IPR033729SerRS_core
IPR006195aa-tRNA-synth_II
IPR002314aa-tRNA-synt_IIb
IPR015866Ser-tRNA-synth_1_N
IPR002317Ser-tRNA-ligase_type_1
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006544 glycine metabolic process
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0006434 seryl-tRNA aminoacylation
biological_process GO:0006566 threonine metabolic process
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004828 serine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25039-RACarg25039-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 49..83
NoneNo IPR availableGENE3DG3DSA:1.10.287.40coord: 1..109
e-value: 5.9E-34
score: 118.6
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 110..446
e-value: 4.7E-143
score: 478.2
NoneNo IPR availablePANTHERPTHR11778:SF7SERINE--TRNA LIGASE, CYTOPLASMICcoord: 2..445
NoneNo IPR availableSUPERFAMILYSSF55681Class II aaRS and biotin synthetasescoord: 121..431
IPR002317Serine-tRNA ligase, type1PRINTSPR00981TRNASYNTHSERcoord: 339..355
score: 61.66
coord: 357..373
score: 65.58
coord: 268..280
score: 66.38
coord: 322..335
score: 45.24
coord: 280..293
score: 59.79
IPR002317Serine-tRNA ligase, type1TIGRFAMTIGR00414TIGR00414coord: 1..424
e-value: 9.5E-148
score: 490.6
IPR002317Serine-tRNA ligase, type1PIRSFPIRSF001529Ser-tRNA_ligasecoord: 1..433
e-value: 1.3E-185
score: 615.0
IPR002317Serine-tRNA ligase, type1PANTHERPTHR11778SERYL-TRNA SYNTHETASEcoord: 2..445
IPR015866Serine-tRNA synthetase, type1, N-terminalPFAMPF02403Seryl_tRNA_Ncoord: 1..112
e-value: 1.2E-25
score: 89.8
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 221..408
e-value: 1.6E-37
score: 129.1
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 142..417
score: 22.29
IPR033729Serine-tRNA ligase catalytic core domainCDDcd00770SerRS_corecoord: 124..424
e-value: 0.0
score: 525.202
IPR010978Class I and II aminoacyl-tRNA synthetase, tRNA-binding armSUPERFAMILYSSF46589tRNA-binding armcoord: 1..117