Carg25014 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg25014
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	tRNA (Guanosine(18)-2'-O)-methyltransferase
Location	Cucurbita_argyrosperma_scaffold_176 : 81771 .. 82872 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTACAGAATAGTATCGACACATCTAGGATCGAATGCGGTATTGATTGATTTTGCAAACTTTCTCCCTTCCACAATGTCCCATGAAGTAGCTTTACATTGAAATCAATTCTGTTCTTATATCGATTGTCTTATGCCAATTTTATTATGTTTCAACCATTTTGAGCTTATCCTGACAGCTGAGTTGTGATTGTCCTGTTTTAGGTTTCCGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGTAATTATCTTCTTTGCTTCAATAATACTGAGTTTGATCAAGTTTTTGTACACTTTTTAAATCTCTGCTAGTTGGGAGATATCAGTTATATTTAGCAAATCATAACTTAGTCCATGTTTAATAAACTGGCCTACACGTTTTGTTTTCCTGCAATTCTAGTCCGCTCATCCTTCGATTAGTGAGTTTCGAGTTGTGAATATTGTCCGAACCAGGAGACATTATACTTGTAGCTAGTTATGACCTTTACCATTTATGTTAGTATCTGAGGTTTAGTTTTCTTTGGTCTCAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCCATGAATGGAATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGATTGGTAACTCTAAAGTCATGTTTGATGTTTTGAATTCGTTTACGCCTAAGTGGTAGTTCAACTAGTTGGGGCATTACCAGGGGTGTTGTCTGGAAATATGTCGAAGGTCCGACGCAAAGGATGTCAAAATGGACATATACTGTTTCTAGTACTGAATTGATGGTATGTTCTTTATCAAACTTTTCAGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCCCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTCGGAGGTGTTGCTGTAG

mRNA sequence

ATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTACAGAATAGTATCGACACATCTAGGATCGAATGCGGTTTCCGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCCATGAATGGAATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGATTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCCCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTCGGAGGTGTTGCTGTAG

Coding sequence (CDS)

ATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTACAGAATAGTATCGACACATCTAGGATCGAATGCGGTTTCCGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGAAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCAGTATTCCCATGAATGGAATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGATTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCCCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTCGGAGGTGTTGCTGTAG

Protein sequence

MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL

Homology

[Close]

BLAST of Carg25014 vs. NCBI nr
Match: XP_022933839.1 (uncharacterized protein LOC111441136 [Cucurbita moschata])

HSP 1 Score: 314.3 bits (804), Expect = 2.3e-82
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG
Sbjct: 222 MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 281

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL
Sbjct: 282 ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 341

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 155
           AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL
Sbjct: 342 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 375

[Close]

BLAST of Carg25014 vs. NCBI nr
Match: XP_023530258.1 (uncharacterized protein LOC111792877 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 314.3 bits (804), Expect = 2.3e-82
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG
Sbjct: 222 MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 281

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL
Sbjct: 282 ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 341

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 155
           AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL
Sbjct: 342 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 375

[Close]

BLAST of Carg25014 vs. NCBI nr
Match: XP_022969711.1 (uncharacterized protein LOC111468788 [Cucurbita maxima])

HSP 1 Score: 310.8 bits (795), Expect = 2.5e-81
Identity = 152/154 (98.70%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG
Sbjct: 222 MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 281

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           ISDEALELSDLHC+IPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL
Sbjct: 282 ISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 341

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 155
           AEFSLRHNKNAISVAHEYAKRKSSSTSSG EVLL
Sbjct: 342 AEFSLRHNKNAISVAHEYAKRKSSSTSSGLEVLL 375

[Close]

BLAST of Carg25014 vs. NCBI nr
Match: KGN59530.1 (hypothetical protein Csa_3G824750 [Cucumis sativus])

HSP 1 Score: 282.3 bits (721), Expect = 9.6e-73
Identity = 139/154 (90.26%), Postives = 149/154 (96.75%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELWNSTPECF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRG
Sbjct: 218 MGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRG 277

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           IS+EALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILL
Sbjct: 278 ISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILL 337

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 155
           AEFSLRH+K+AIS+A+E AKRK S+ SS +EVLL
Sbjct: 338 AEFSLRHSKSAISIANELAKRKGSAISS-TEVLL 370

[Close]

BLAST of Carg25014 vs. NCBI nr
Match: XP_004136796.2 (PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus])

HSP 1 Score: 282.3 bits (721), Expect = 9.6e-73
Identity = 139/154 (90.26%), Postives = 149/154 (96.75%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELWNSTPECF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRG
Sbjct: 261 MGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRG 320

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           IS+EALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILL
Sbjct: 321 ISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILL 380

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 155
           AEFSLRH+K+AIS+A+E AKRK S+ SS +EVLL
Sbjct: 381 AEFSLRHSKSAISIANELAKRKGSAISS-TEVLL 413

[Close]

BLAST of Carg25014 vs. TAIR10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 242.3 bits (617), Expect = 2.0e-64
Identity = 111/148 (75.00%), Postives = 132/148 (89.19%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIE W++  ECF++LKSRGYRI +THLG + VS+YDMDWSCPTAIVVGNE RG
Sbjct: 202 MGAEKWLDIEFWDTPKECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRG 261

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           ISDEALELSDL CSIPMNGMVDSFNVSVAAGILMHHAV DRT+RLG HGDL+  E++IL+
Sbjct: 262 ISDEALELSDLRCSIPMNGMVDSFNVSVAAGILMHHAVSDRTTRLGSHGDLSEAEKEILM 321

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSS 149
           AEFSLRH++++I +A+E+AKRK   ++S
Sbjct: 322 AEFSLRHSRSSICIAYEFAKRKQQHSTS 349

[Close]

BLAST of Carg25014 vs. TAIR10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 40.8 bits (94), Expect = 8.8e-04
Identity = 20/47 (42.55%), Postives = 32/47 (68.09%), Query Frame = 0


Query: 49  PTAIVVGNESRGISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMH 96
           P  +++G+E  G+S++A ++  L  SIPM G  +S NVSVA GI ++
Sbjct: 301 PLCLILGSEGNGLSEQARKVCVL-VSIPMAGDFESLNVSVAGGIFLY 346

[Close]

BLAST of Carg25014 vs. Swiss-Prot
Match: sp|O67577|TRMH_AQUAE (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trmH PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.5e-16
Identity = 41/103 (39.81%), Postives = 66/103 (64.08%), Query Frame = 0


Query: 2   GAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGI 61
           G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT +VVGNE +G+
Sbjct: 73  GSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPTVLVVGNELQGV 132

Query: 62  SDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR 105
           S E +E++D    IPM GM  S NVSVA GI+++ A   R  +
Sbjct: 133 SPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEK 175

[Close]

BLAST of Carg25014 vs. Swiss-Prot
Match: sp|P0AGJ3|TRMH_ECO57 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334 GN=trmH PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.3e-16
Identity = 46/124 (37.10%), Postives = 72/124 (58.06%), Query Frame = 0


Query: 2   GAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGI 61
           G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E  GI
Sbjct: 66  GSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGI 125

Query: 62  SDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSIERQI 121
           + EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   E+Q 
Sbjct: 126 TQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEAEQQR 185

Query: 122 LLAE 123
           LL E
Sbjct: 186 LLFE 189

[Close]

BLAST of Carg25014 vs. Swiss-Prot
Match: sp|P0AGJ2|TRMH_ECOLI (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmH PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.3e-16
Identity = 46/124 (37.10%), Postives = 72/124 (58.06%), Query Frame = 0


Query: 2   GAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGI 61
           G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E  GI
Sbjct: 66  GSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGI 125

Query: 62  SDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSIERQI 121
           + EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   E+Q 
Sbjct: 126 TQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEAEQQR 185

Query: 122 LLAE 123
           LL E
Sbjct: 186 LLFE 189

[Close]

BLAST of Carg25014 vs. Swiss-Prot
Match: sp|P0AGJ4|TRMH_SHIFL (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.3e-16
Identity = 46/124 (37.10%), Postives = 72/124 (58.06%), Query Frame = 0


Query: 2   GAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGI 61
           G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E  GI
Sbjct: 66  GSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGI 125

Query: 62  SDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSIERQI 121
           + EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   E+Q 
Sbjct: 126 TQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEAEQQR 185

Query: 122 LLAE 123
           LL E
Sbjct: 186 LLFE 189

[Close]

BLAST of Carg25014 vs. Swiss-Prot
Match: sp|Q72GI1|TRMH_THET2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) OX=262724 GN=trmH PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.1e-15
Identity = 40/96 (41.67%), Postives = 60/96 (62.50%), Query Frame = 0


Query: 2   GAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGI 61
           G+ KW+ + +     E FR LK RG+ + +T L  +A    ++D++ PTA++ G E  G+
Sbjct: 69  GSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGAEKWGV 128

Query: 62  SDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 98
           S+EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 129 SEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

[Close]

BLAST of Carg25014 vs. TrEMBL
Match: tr|A0A0A0LC15|A0A0A0LC15_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 6.4e-73
Identity = 139/154 (90.26%), Postives = 149/154 (96.75%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELWNSTPECF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRG
Sbjct: 218 MGAEKWLDIELWNSTPECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRG 277

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           IS+EALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVC+RTSRLGCHGDLTS ERQILL
Sbjct: 278 ISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILL 337

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVLL 155
           AEFSLRH+K+AIS+A+E AKRK S+ SS +EVLL
Sbjct: 338 AEFSLRHSKSAISIANELAKRKGSAISS-TEVLL 370

[Close]

BLAST of Carg25014 vs. TrEMBL
Match: tr|A0A1S3B8M3|A0A1S3B8M3_CUCME (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486969 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.4e-72
Identity = 136/153 (88.89%), Postives = 145/153 (94.77%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELWNST ECF+ILKSRGYRIVSTHLG+NAVSVYDMDWSCPTAIVVGNESRG
Sbjct: 217 MGAEKWLDIELWNSTSECFKILKSRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRG 276

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           IS+EALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTS ERQILL
Sbjct: 277 ISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILL 336

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSSTSSGSEVL 154
           AEFSLRH+K+AIS+A+E AKRK S+ SS   +L
Sbjct: 337 AEFSLRHSKSAISIANELAKRKGSAISSTGVLL 369

[Close]

BLAST of Carg25014 vs. TrEMBL
Match: tr|A0A2C9WB01|A0A2C9WB01_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G050600 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.8e-68
Identity = 125/145 (86.21%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELW+ST ECF +LKSRGYRI +TH+G +AVSVYDMDWSCPTAIVVGNE+RG
Sbjct: 218 MGAEKWLDIELWDSTQECFEVLKSRGYRIATTHVGMDAVSVYDMDWSCPTAIVVGNENRG 277

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           ISDEALELSDLHCSIPM GMVDSFNVSVAAGILMHHAVCDR+SRLGCHGDLTS E QILL
Sbjct: 278 ISDEALELSDLHCSIPMKGMVDSFNVSVAAGILMHHAVCDRSSRLGCHGDLTSEESQILL 337

Query: 121 AEFSLRHNKNAISVAHEYAKRKSSS 146
           AEFSLRH+K+AIS+AHEYAKRK+++
Sbjct: 338 AEFSLRHSKSAISIAHEYAKRKAAT 362

[Close]

BLAST of Carg25014 vs. TrEMBL
Match: tr|A0A251Q5N4|A0A251Q5N4_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G259900 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.2e-68
Identity = 123/144 (85.42%), Postives = 137/144 (95.14%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELW+STPECF +LKSRGYRI +TH+G +AVSVYDMDWSCPTAIVVGNE+RG
Sbjct: 214 MGAEKWLDIELWDSTPECFEVLKSRGYRIATTHVGMDAVSVYDMDWSCPTAIVVGNENRG 273

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           ISDEAL+LSDLHCSIPM GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLT  E QILL
Sbjct: 274 ISDEALDLSDLHCSIPMKGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTFEESQILL 333

Query: 121 AEFSLRHNKNAISVAHEYAKRKSS 145
           AEFSLRH+K+++S+AHEYAKRK++
Sbjct: 334 AEFSLRHSKSSMSIAHEYAKRKAA 357

[Close]

BLAST of Carg25014 vs. TrEMBL
Match: tr|M5WSN7|M5WSN7_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa008118mg PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.2e-68
Identity = 123/144 (85.42%), Postives = 137/144 (95.14%), Query Frame = 0


Query: 1   MGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRG 60
           MGAEKWLDIELW+STPECF +LKSRGYRI +TH+G +AVSVYDMDWSCPTAIVVGNE+RG
Sbjct: 195 MGAEKWLDIELWDSTPECFEVLKSRGYRIATTHVGMDAVSVYDMDWSCPTAIVVGNENRG 254

Query: 61  ISDEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILL 120
           ISDEAL+LSDLHCSIPM GMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLT  E QILL
Sbjct: 255 ISDEALDLSDLHCSIPMKGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTFEESQILL 314

Query: 121 AEFSLRHNKNAISVAHEYAKRKSS 145
           AEFSLRH+K+++S+AHEYAKRK++
Sbjct: 315 AEFSLRHSKSSMSIAHEYAKRKAA 338

The following BLAST results are available for this feature:

BLAST of Carg25014 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022933839.1	2.3e-82	100.00	uncharacterized protein LOC111441136 [Cucurbita moschata]	[more]
XP_023530258.1	2.3e-82	100.00	uncharacterized protein LOC111792877 [Cucurbita pepo subsp. pepo]	[more]
XP_022969711.1	2.5e-81	98.70	uncharacterized protein LOC111468788 [Cucurbita maxima]	[more]
KGN59530.1	9.6e-73	90.26	hypothetical protein Csa_3G824750 [Cucumis sativus]	[more]
XP_004136796.2	9.6e-73	90.26	PREDICTED: uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus]	[more]

BLAST of Carg25014 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G15390.1	2.0e-64	75.00	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT4G38020.1	8.8e-04	42.55	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

BLAST of Carg25014 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O67577\|TRMH_AQUAE	2.5e-16	39.81	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=...	[more]
sp\|P0AGJ3\|TRMH_ECO57	4.3e-16	37.10	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334...	[more]
sp\|P0AGJ2\|TRMH_ECOLI	4.3e-16	37.10	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=...	[more]
sp\|P0AGJ4\|TRMH_SHIFL	4.3e-16	37.10	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH ...	[more]
sp\|Q72GI1\|TRMH_THET2	2.1e-15	41.67	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain HB27...	[more]

BLAST of Carg25014 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LC15\|A0A0A0LC15_CUCSA	6.4e-73	90.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1	[more]
tr\|A0A1S3B8M3\|A0A1S3B8M3_CUCME	5.4e-72	88.89	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486...	[more]
tr\|A0A2C9WB01\|A0A2C9WB01_MANES	2.8e-68	86.21	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G050600 PE=3 SV=...	[more]
tr\|A0A251Q5N4\|A0A251Q5N4_PRUPE	6.2e-68	85.42	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G259900 PE=3 SV=1	[more]
tr\|M5WSN7\|M5WSN7_PRUPE	6.2e-68	85.42	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa008118mg PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0008173	RNA methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006396	RNA processing

Vocabulary: **INTERPRO**
Term	Definition
IPR029028	Alpha/beta_knot_MTases
IPR029026	tRNA_m1G_MTases_N
IPR001537	SpoU_MeTrfase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009451	RNA modification
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25014-RA	Carg25014-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 1..95 e-value: 1.7E-23 score: 83.2
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	G3DSA:3.40.1280.10		coord: 1..138 e-value: 2.1E-38 score: 133.5
None	No IPR available	PANTHER	PTHR43453	FAMILY NOT NAMED	coord: 1..146
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 1..146
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	SSF75217	alpha/beta knot	coord: 1..104

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg25014	Melon (DHL92) v3.6.1	carmedB0259
Carg25014	Watermelon (97103) v2	carwmbB0251
Carg25014	Wax gourd	carwgoB0313
Carg25014	Cucumber (Gy14) v2	carcgybB0261
Carg25014	Cucurbita maxima (Rimu)	carcmaB0342
Carg25014	Cucurbita moschata (Rifu)	carcmoB0346
Carg25014	Cucurbita pepo (Zucchini)	carcpeB0347
Carg25014	Wild cucumber (PI 183967)	carcpiB0278
Carg25014	Cucumber (Chinese Long) v2	carcuB0274
Carg25014	Cucumber (Chinese Long) v3	carcucB0277
Carg25014	Bottle gourd (USVL1VR-Ls)	carlsiB220