Carg24449 (gene) Silver-seed gourd

NameCarg24449
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionaminoacylase-1-like
LocationCucurbita_argyrosperma_scaffold_353 : 135139 .. 139194 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAACAATTATGTACCGGAGCAGCACCGGTCTTCTGTCGCCGGCGAGTTGAAGAGGCAAAAAGCTAAGTTTTGACAATCCATTCATCGGCGGTTGTCTTCACCATTCGGCAGCTCTCCGGCTTACTCAACTTCAGAAGTTCCGACTTGCTCAACTGCTTGACCGCGGCACCAACGGAGGCGCCCATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTCATTTTCTCTCTCTTTCAGTCATTCGCAGTACGATGATGATGAATCCGATCAAAATTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCTGTCTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTCGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGATTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCGTCCGGACCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCTATTCGGAACCTCCGAAGTCGGAATTTCGTCCCTGACCGTACGATTCACATATCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGATAATTAGGGCTGAAGGAACCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGTGAGTAGTCCTTGTGAAATGAGTTGCAGGGTTCAAGTCCTTCTGCCTATGTCTCTTTTAGCCATTAATATTGTGATTTGTCTCAGGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCCACCGCCGACCCAGATGCTATTAGAAGAAGAATTGTGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAAGTAAGTGGCTAGCTGCCTGATTTGATTCCCAACACCATCTGGTTCCTACCCAATTTTGTTTTGATTCTGGTATTCATCTTTTTCTTTTGAGGTCCCCTGTTTTATTAGCTCCCATCATTAGTATTCTGTTCTTGTTCATGGCTTATGGTTCTGCAATCTAGAGAAAAAAGCTTGGAGAAGTCCTTATATGACTTGTCATTTTATCTTGATGAAAACAGAGTAAATATGTAAGATTATGATCTTAAACCAATTTTGATGTGGAATGAATTTGAATTCTGATGGAGGATGTTTTTGTTTGTTCTATATGTTATATTATGAAACTCGTCTACTCCATCCACAACAAAATGCACCGGGCAATGGCATGCGAATTTTCCCTTGTCATGGGAGGAATTGATTAGATTTTCTCATTTCTTGTGAGATCCAATATCGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAATCTCTCTCTAGCAGATACGTTTTGAAAACCTTGAGGGGAAACTCGAAAGGGAAAGCCCAAAGAGGACAATATTTGTTAGCGGTGGGTTTAGGCCGTTACAAATGGTATTAGAGCCAGACATTAGGCGATGTGCCAATGCAGAGGCGGAGTCCCGAAGGGGGTGGACACGAGGCGGTGTGCTAGCAAGGACGCTGGCCCTCGAAGGGGGTGGACTGTAAGATCCCACATCAGCTGGAGAGGGGAACAAAACGTTCTTTATAAGGTGTGGAAACCTCTCCCTAACAAACGCGTTTTTAAATCTTGAGGGGAAGCCCGAAAGGGAAAGCTCAAACAGGACAAGGGCTTGGGCCGTTACACTTCTGCTCTACGTACCCGAGATTGAATGCTTGTTTTATGCATGTCTAGCCTTTAGCAATTAGATATTCAAGTATTTGCATCATATAGCAGTCTCTTACGAATTACACACTATCTATTTGTACGTTATCTTAGCAATGTCTGATAGCTTAAACATAATGGATCTGATACCTGCAGATTATTGAGAAAGGACCCATTAGAGACTATTTGGGACGTCCATTATTGACGATGACTAACGATTCGAATCCGTGGTGGTCTGTTTTCAAGACAGCCATCTCAGATGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACAACTGATGCACGCTTCATGAGACAGTTAGCGATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGAACACAACGAGGTTATACTACTCCATTGTATCTCTCTTTTTTATCCGTGTTCTTGCTCGATTTGCGTTATATTCGTACTAATCGTCATCTCCTTTCCTCGTTCCTTGTTGCAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAAATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCTGTAAGTGCTCCCATGGATGAACACTGCTATTGTAAGTACTGAAGTTCTCTTTTCCACACAACGTCACAATTCATTTGTAAACATTCTCTTCCGTTTTATTACCTTCTCATGAGATTCGAATAGGGTAAATAATGAACAAAAAAAATGATCTTTTGTAGTATATTGAGAGTGTCTCCTCTAAGCGCATCCTTCCTAAATTAAATGGGTATCATGGGTATCAAATAACGAGATCTATTCTATAGGTCGTAAAAATGTGTCCTCGAATTCTAAGAAGTTATATAGCTATCAACAATGAAATGTATATTGTTCGAAATAGAAGTCATGTGTCGTACACCTTACTCGACATGCTAGGACAATTTGAGGGGCTAGCTCGAGCATAAACGAAGAATTGAATTACCGTGCTTAGATTGGGTCTTGTGGTTCTAACGTTGAGATTCTATTTAGAGAGGGTCTCCATGTTTGTAGGTGCACTCAAATGAGAAAACTTAAAAGGTCAACAAGGAGACGTATATAGTATTGCAATGCTAGTAGCTAATAAATTCTACGTACTTGCACATTCATCGTAGTGCCTCAACTTAGTTTATAGCATCCCAACATTGCCGCACAATTTCTTATAAAATGTAGATTTGTTAGTGCCTCTAAGGGATAAATAGTGGGTAGAAAAGTTGACCATCAACGATTGAGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACCATTAGAGAGTGGTGGAAAGAAATTTTGGAGGTTCGAATTGTTTTGGGTCTCTGAAAGGTGCATTTCTATACGTGGTGGGGTTCGTGGGTCATTGATTTTTAACTCATGCTAGAACATGTAGGTCAAGGGGCAGTCTTAAGTAGACAATTTCCTTTTATTTTCTTTTTATTTGAAAAATATAGATGGTTCTTTTTTACTCTCTGCCACATCATCCTCCAACACTCAATCAGATCCTGCCATTTGGCCACTAAACTCTGTAACTCGACAGCCACCCAACAAAGAAAATGGGTCTTGTAATTGCTGGTCCACCTGAAACTTACCATTCAGTGGCTGCAGATCAGAATTCCCAAGCTCTTCTGAAACACTTCCAGAGGATTCTGGCCATTCATAATAAACCTCAGAGCTCCGGTGGGCTTCAATGGTTGGCTCTGAGGAGGATGAAGCAGAAGGGTTTTGCAGCAGAGTGTGAGAAGCTCCGTGAAAATGGAGAATTGTAGTTATAGGAGGTTGATGATGATTTTTTATCCATTTTTTGGAGCAACCTAGGAATCCACAAATGATGAACTGTCTCTAAGAACTGTTGGCTGTTTGAGTTGATGTTGAGCTGTTTTGCTTGTTT

mRNA sequence

GTAAACAATTATGTACCGGAGCAGCACCGGTCTTCTGTCGCCGGCGAGTTGAAGAGGCAAAAAGCTAAGTTTTGACAATCCATTCATCGGCGGTTGTCTTCACCATTCGGCAGCTCTCCGGCTTACTCAACTTCAGAAGTTCCGACTTGCTCAACTGCTTGACCGCGGCACCAACGGAGGCGCCCATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTCATTTTCTCTCTCTTTCAGTCATTCGCAGTACGATGATGATGAATCCGATCAAAATTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCTGTCTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTCGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGATTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCGTCCGGACCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCTATTCGGAACCTCCGAAGTCGGAATTTCGTCCCTGACCGTACGATTCACATATCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGATAATTAGGGCTGAAGGAACCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCCACCGCCGACCCAGATGCTATTAGAAGAAGAATTGTGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAAATTATTGAGAAAGGACCCATTAGAGACTATTTGGGACGTCCATTATTGACGATGACTAACGATTCGAATCCGTGGTGGTCTGTTTTCAAGACAGCCATCTCAGATGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACAACTGATGCACGCTTCATGAGACAGTTAGCGATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGAACACAACGAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAAATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCTGTAAGTGCTCCCATGGATGAACACTGCTATTTGGCTGCAGATCAGAATTCCCAAGCTCTTCTGAAACACTTCCAGAGGATTCTGGCCATTCATAATAAACCTCAGAGCTCCGGTGGGCTTCAATGGTTGGCTCTGAGGAGGATGAAGCAGAAGGGTTTTGCAGCAGAGTGTGAGAAGCTCCGTGAAAATGGAGAATTGTAGTTATAGGAGGTTGATGATGATTTTTTATCCATTTTTTGGAGCAACCTAGGAATCCACAAATGATGAACTGTCTCTAAGAACTGTTGGCTGTTTGAGTTGATGTTGAGCTGTTTTGCTTGTTT

Coding sequence (CDS)

ATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTCATTTTCTCTCTCTTTCAGTCATTCGCAGTACGATGATGATGAATCCGATCAAAATTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCTGTCTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTCGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGATTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCGTCCGGACCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCTATTCGGAACCTCCGAAGTCGGAATTTCGTCCCTGACCGTACGATTCACATATCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGATAATTAGGGCTGAAGGAACCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCCACCGCCGACCCAGATGCTATTAGAAGAAGAATTGTGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAAATTATTGAGAAAGGACCCATTAGAGACTATTTGGGACGTCCATTATTGACGATGACTAACGATTCGAATCCGTGGTGGTCTGTTTTCAAGACAGCCATCTCAGATGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACAACTGATGCACGCTTCATGAGACAGTTAGCGATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGAACACAACGAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAAATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCTGTAA

Protein sequence

MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASL
BLAST of Carg24449 vs. NCBI nr
Match: XP_023553812.1 (aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553821.1 aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553828.1 aminoacylase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840.5 bits (2170), Expect = 2.7e-240
Identity = 420/448 (93.75%), Postives = 421/448 (93.97%), Query Frame = 0

Query: 1   MANFRLHCRLFSLAXXXXXXXXXXXXXXXXXXXXXXXXNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLA                        NS ISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVILFLFSFPLSFSDSKYVDDESDQNSSISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180
           LYPPFSAV TSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVLTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASL 449
           KDTVFLRGIKIYESIISALSSFVADASL
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASL 448

BLAST of Carg24449 vs. NCBI nr
Match: XP_022991055.1 (aminoacylase-1 [Cucurbita maxima])

HSP 1 Score: 838.6 bits (2165), Expect = 1.0e-239
Identity = 439/447 (98.21%), Postives = 443/447 (99.11%), Query Frame = 0

Query: 1   MANFRLHCRLFSLAXXXXXXXXXXXXXXXXXXXXXXXXNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAXXXXXXXXXXXXXXXXXXXXXXX NSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAXXXXXXXXXXXXXXXXXXXXXXXQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180
           LYPPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEG PGHGSRMY
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
           KDTVFLRGIKIYESIISALSSFVADAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

BLAST of Carg24449 vs. NCBI nr
Match: XP_022964785.1 (aminoacylase-1 [Cucurbita moschata])

HSP 1 Score: 837.8 bits (2163), Expect = 1.7e-239
Identity = 437/447 (97.76%), Postives = 439/447 (98.21%), Query Frame = 0

Query: 1   MANFRLHCRLFSLAXXXXXXXXXXXXXXXXXXXXXXXXNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLA  XXXXXXXXXXXXXXXXXXX   NSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFXXXXXXXXXXXXXXXXXXXSDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180
           LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
           KDTVFLRGIKIYESIISALSSFVADAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

BLAST of Carg24449 vs. NCBI nr
Match: KGN63599.1 (hypothetical protein Csa_1G005600 [Cucumis sativus])

HSP 1 Score: 745.7 bits (1924), Expect = 8.9e-212
Identity = 367/449 (81.74%), Postives = 399/449 (88.86%), Query Frame = 0

Query: 1   MANFRLHCRLFS--LAXXXXXXXXXXXXXXXXXXXXXXXXNSPISRFQHYLRIKTAHPDP 60
           M+NF LH RLFS  L                         +SP+SRFQ YLRIKTAHPDP
Sbjct: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSQSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPS 120
           DYASAV+FL SQA EIGLHT+ILEFV GKPLLLVTW GL+PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGLDPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEI 180
           KW++PPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR+R+FVP RTIHISYVPDEEI
Sbjct: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180

Query: 181 GGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSR 240
           GG DGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A+G+PGHGSR
Sbjct: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300
           MYDNSAMENLMKSVEI+++FRESQF++VKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTN 360
           QPSEAEAGFDLR+PPTADPDA+RRRI EEWAPARRN+T QI+EKGPIRDYLG PL+TMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDYLGSPLMTMTN 360

Query: 361 DSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNE 420
            SNPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNE
Sbjct: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLKDTVFLRGIKIYESIISALSSFVADAS 448
           FL D+VF+RGIK+YES+ISALSSF  D S
Sbjct: 421 FLSDSVFIRGIKVYESLISALSSFQEDVS 449

BLAST of Carg24449 vs. NCBI nr
Match: XP_022141532.1 (aminoacylase-1 [Momordica charantia] >XP_022141534.1 aminoacylase-1 [Momordica charantia])

HSP 1 Score: 744.6 bits (1921), Expect = 2.0e-211
Identity = 371/447 (83.00%), Postives = 393/447 (87.92%), Query Frame = 0

Query: 1   MANFRLHCRLFSLAXXXXXXXXXXXXXXXXXXXXXXXXNSPISRFQHYLRIKTAHPDPDY 60
           MAN   HC   SLA                        +SP+SRFQ+YLRIKTAHP+PDY
Sbjct: 1   MANLHHHCSFISLA--AFLLLLSFPLSFCHLQDDEVDPDSPVSRFQNYLRIKTAHPNPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           A AVSFL+SQAL IGL  +ILEFV GKPLLL+TW G NPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  AFAVSFLKSQALAIGLDAQILEFVEGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180
           L+PPFSAVR+SDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVP R+IHISYVPDEEIGG
Sbjct: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240
           FDGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRA GTPGHGS +Y
Sbjct: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFDMVKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLR+PPTADPDAIRRRI EEWAPARRN+T QI ++GPIRDYLGRPL+TMTN S
Sbjct: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420
           N WWSVF+TAISDAGGKLS PEILASTTDARFMRQL IPVLGFSPMSNTPILLH+HNEFL
Sbjct: 361 NRWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
            +TVFLRGIK+YESIISALSSF   AS
Sbjct: 421 SETVFLRGIKVYESIISALSSFEEGAS 445

BLAST of Carg24449 vs. TAIR10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein)

HSP 1 Score: 628.2 bits (1619), Expect = 3.8e-180
Identity = 288/409 (70.42%), Postives = 350/409 (85.57%), Query Frame = 0

Query: 39  NSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLN 98
           ++PI+RFQ YLR  TAHP+P+Y + +SFL +QA  IGL TK +EF+ GKP+LL+TW G N
Sbjct: 27  DTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTTKTIEFISGKPILLITWLGSN 86

Query: 99  PSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR 158
           P+LPSIL NSH+DSVPAE  KW YPPFSA +T DG I+ARG+QDDKCI +QYLE+IRNL+
Sbjct: 87  PNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLESIRNLK 146

Query: 159 SRNFVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYA 218
           SR F P RTIHISYVP+EEIGGFDG  KF  S EFK+LN+GF MDEGQA+PGDEFRVFYA
Sbjct: 147 SRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNLGFAMDEGQANPGDEFRVFYA 206

Query: 219 DRSPWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVIS 278
           DR PW  +I+AEG PGHG+++YDNSAMENLMKSVE+IS+FRESQFD VKAG+AA SEVIS
Sbjct: 207 DRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELISRFRESQFDFVKAGKAAYSEVIS 266

Query: 279 VNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQ 338
           VNPV++ AG P+ TGFVMNMQPSEAEAG+DLR+PP ADPD +++RI EEWAP+ RN+T  
Sbjct: 267 VNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMADPDVMKKRIAEEWAPSIRNMTYS 326

Query: 339 IIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAI 398
           I EKG +RD+LGRP++T  NDSNPWWS+FK A+   GGKL++PEILASTTD+RF+R L I
Sbjct: 327 IQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMGGKLAKPEILASTTDSRFIRTLGI 386

Query: 399 PVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADAS 448
           P  GFSPM+NTPILLH+HNEFLKDTVF++GI++YES+ISALSSF  +++
Sbjct: 387 PTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESVISALSSFEGESA 435

BLAST of Carg24449 vs. TAIR10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein)

HSP 1 Score: 614.8 bits (1584), Expect = 4.3e-176
Identity = 285/406 (70.20%), Postives = 347/406 (85.47%), Query Frame = 0

Query: 39  NSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLN 98
           ++PI+RFQ YLR  TAHP+P+Y + +SFL  QA  IGL ++ +EFVPGKP+LL+TW G N
Sbjct: 29  DTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIGLTSRTIEFVPGKPVLLLTWLGSN 88

Query: 99  PSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR 158
            +L SIL NSH+DSVPAE  KW++PPFSA RT DG I+ARG+QDDKCI +QYLEAIRNL+
Sbjct: 89  LNLSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGHIYARGAQDDKCIGVQYLEAIRNLK 148

Query: 159 SRNFVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYA 218
           SR+F P RTIHISYVP+EEIGGF G  KF  S EFK+LN+GF+MDEGQASPGDEFRVFYA
Sbjct: 149 SRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSEFKDLNLGFVMDEGQASPGDEFRVFYA 208

Query: 219 DRSPWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVIS 278
           +R+PW L IRA G PGHG+++YDNSAMENLMKSVE+IS+FRESQFD VKAG+AANSEVIS
Sbjct: 209 ERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSVELISRFRESQFDFVKAGKAANSEVIS 268

Query: 279 VNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQ 338
           VNPV++ AG PS TGFVMNMQPSEAE G+DLR+PP ADP A+++RI EEWAP+ RN+T  
Sbjct: 269 VNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLPPMADPVALKKRIAEEWAPSIRNMTYT 328

Query: 339 IIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAI 398
           + ++G + D+LGRP++T TND+NPWWS+FK A+   GGKL++PEIL STTD+RF+R L I
Sbjct: 329 LKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVEATGGKLAKPEILISTTDSRFIRTLGI 388

Query: 399 PVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           PVLGFSPM NTPIL+H+HNEFLKDTVF++GI +YES+ISALSSF A
Sbjct: 389 PVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVYESVISALSSFEA 434

BLAST of Carg24449 vs. TAIR10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein)

HSP 1 Score: 484.2 bits (1245), Expect = 8.8e-137
Identity = 223/402 (55.47%), Postives = 299/402 (74.38%), Query Frame = 0

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           +SRFQ YLRI T  P+P+Y  AV F+ SQA  + L ++ +EFV GKPLLL+ W G +P+L
Sbjct: 25  VSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSDPTL 84

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
           P+ LLNSH D VP E SKW + P  A     G I+ARGSQD KC+ +QYLEAIR L++  
Sbjct: 85  PAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQASG 144

Query: 162 FVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
           F P R++++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RVFY +RS
Sbjct: 145 FKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERS 204

Query: 222 PWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNP 281
           PW L+I+A+G PGHG+++YDNSAMENL+KS+E I +FR SQFD++KAG  A  +V+SVN 
Sbjct: 205 PWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVSVNM 264

Query: 282 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIE 341
            F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+VEEWAPA RN++    E
Sbjct: 265 AFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMS---FE 324

Query: 342 KGPIRDYL-GRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPV 401
            G  +  L G+  LT  +DSNPWW + + A+ +AGG+ S+PEI  ++TDAR+ R+  +P 
Sbjct: 325 LGQFKQKLTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGVPA 384

Query: 402 LGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSF 443
            GFSP+SNTP LLH+HNE+L    +L+GI++Y SII A +S+
Sbjct: 385 FGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASY 423

BLAST of Carg24449 vs. Swiss-Prot
Match: sp|Q99JW2|ACY1_MOUSE (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.1e-91
Identity = 172/401 (42.89%), Postives = 242/401 (60.35%), Query Frame = 0

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           ++ F+ YLRI T  P+PDY  A++FLE +A ++GL  + +E VPG  + ++TW G NPSL
Sbjct: 13  VTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLTWPGTNPSL 72

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
           PSILLNSH D VP     W + PF A + S+G I+ARGSQD K ++IQYLEA+R L+S  
Sbjct: 73  PSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEAVRRLKSEG 132

Query: 162 FVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
               RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERS 192

Query: 222 PWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAANSEVISVN 281
           PW + + + G PGH SR  +++A E L K +  I  FRE +   ++A        V SVN
Sbjct: 193 PWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHLKEGAVTSVN 252

Query: 282 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQII 341
              +  G+        N+ P+   A FD R+ P  D  A  +++      A   +T +  
Sbjct: 253 LTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQEAGEGVTFEFA 312

Query: 342 EKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPV 401
           +K         P +T T+DS+PWW+ F  A   A      PEI  + TD+R++R + IP 
Sbjct: 313 QK------FTEPRMTPTDDSDPWWAAFSGACK-AMNLTLEPEIFPAATDSRYIRAVGIPA 372

Query: 402 LGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSS 442
           LGFSPM+ TP+LLH+HNE L + +FLRG+ IY  ++SAL+S
Sbjct: 373 LGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

BLAST of Carg24449 vs. Swiss-Prot
Match: sp|P37111|ACY1_PIG (Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 3.1e-91
Identity = 169/403 (41.94%), Postives = 243/403 (60.30%), Query Frame = 0

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           ++ F+ YLRI+T  P+PDY +AV+FLE +A ++GL  + +E VPG  + ++TW G NP+L
Sbjct: 13  VTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPGHVVTVLTWPGTNPTL 72

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
            SILLNSH D VP     W + PF   + +DG I+ RG+QD KC++IQYLEA+R L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRLKVEG 132

Query: 162 FVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
               RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG ASP D F VFY++RS
Sbjct: 133 HHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFYSERS 192

Query: 222 PWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNP 281
           PW L + + G PGHGSR  +++A E L K +  I  FRE +   +++ +     V SVN 
Sbjct: 193 PWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVTSVNL 252

Query: 282 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIE 341
             +  G+        N+ P+   A FD R+ P  D  A   ++      A   +T + ++
Sbjct: 253 TMLEGGV------AYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTFEFVQ 312

Query: 342 KGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVL 401
           K           +T T+DS+PWW+ F     D    L   EI  ++TDAR++R   +P L
Sbjct: 313 KWM------ETQVTSTDDSDPWWAAFSGVFKDMKLALEL-EICPASTDARYIRAAGVPAL 372

Query: 402 GFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           GFSPM++TP+LLH+H+E L + VFLRG+ IY  ++SAL+S  A
Sbjct: 373 GFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALASVPA 402

BLAST of Carg24449 vs. Swiss-Prot
Match: sp|Q6AYS7|ACY1A_RAT (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.0e-89
Identity = 171/404 (42.33%), Postives = 242/404 (59.90%), Query Frame = 0

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           ++ F+ YLRI T  P+PDY SAV+FLE +A ++GL  + +E  PG  + ++TW G NP L
Sbjct: 13  VTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGTNPLL 72

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
            SILLNSH D VP     W + PF A + S+G I+ARG+QD K ++IQYLEA+R L+S  
Sbjct: 73  HSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRLKSEG 132

Query: 162 FVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
               RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERS 192

Query: 222 PWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAANSEVISVN 281
           PW + + + G PGH SR  +++A E L K V  I  FRE +   ++A        V SVN
Sbjct: 193 PWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAVTSVN 252

Query: 282 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQII 341
              +  G+        N+ P+   A FD R+ P  D  A  +++      A   +T +  
Sbjct: 253 LTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVTFEFA 312

Query: 342 EKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPV 401
           +K         P +T T+D++PWW+ F  A  +    L  PEI  + TD+R++R + IP 
Sbjct: 313 QK------FTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRYIRAVGIPA 372

Query: 402 LGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           LGFSPM+ TP+LLH+HNE L + VFLRG+ IY  +++AL+S  A
Sbjct: 373 LGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPA 403

BLAST of Carg24449 vs. Swiss-Prot
Match: sp|Q5RFB0|ACY1_PONAB (Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2)

HSP 1 Score: 330.1 bits (845), Expect = 3.8e-89
Identity = 170/404 (42.08%), Postives = 239/404 (59.16%), Query Frame = 0

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           ++ F+ YLRI+T  P PDY +AV+F E +A ++GL  + +E  PG  + ++TW G NP+L
Sbjct: 13  VTLFRQYLRIRTVQPKPDYGAAVAFFEERARQLGLGCQKVEVAPGYVVTVLTWPGTNPTL 72

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
            SILLNSH D VP     W + PF A + S+G I+ARG+QD KCI+IQYLEA+R L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCISIQYLEAVRRLKVEG 132

Query: 162 FVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
               RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERS 192

Query: 222 PWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAANSEVISVN 281
           PW + + + G PGH SR  +++A E L K V  I  FRE ++  +++        V SVN
Sbjct: 193 PWWVRVTSTGRPGHASRFMEDTAAEKLHKVVSSILAFREKEWQRLQSNPHLKEGSVTSVN 252

Query: 282 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQII 341
              +  G+        N+ P+   A FD R+ P  D  A   ++      A   +T +  
Sbjct: 253 LTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFA 312

Query: 342 EKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPV 401
           +K         P +T T+DSNPWW+ F     D    L  PEI+ + TD R++R + IP 
Sbjct: 313 QKWM------HPQVTPTDDSNPWWAAFSRVCKDMKLTL-EPEIMPAATDNRYIRAVGIPA 372

Query: 402 LGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           LGFSPM+ TP+LLH+H+E L + VFLRG+ IY  ++ AL+S  A
Sbjct: 373 LGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPA 403

BLAST of Carg24449 vs. Swiss-Prot
Match: sp|Q03154|ACY1_HUMAN (Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 6.5e-89
Identity = 168/404 (41.58%), Postives = 238/404 (58.91%), Query Frame = 0

Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           ++ F+ YLRI+T  P PDY +AV+F E  A ++GL  + +E  PG  + ++TW G NP+L
Sbjct: 13  VTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNPTL 72

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 161
            SILLNSH D VP     W + PF A + S+G I+ARG+QD KC++IQYLEA+R L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYLEAVRRLKVEG 132

Query: 162 FVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
               RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERS 192

Query: 222 PWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAANSEVISVN 281
           PW + + + G PGH SR  +++A E L K V  I  FRE ++  +++        V SVN
Sbjct: 193 PWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVN 252

Query: 282 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQII 341
              +  G+        N+ P+   A FD R+ P  D  A   ++      A   +T +  
Sbjct: 253 LTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFA 312

Query: 342 EKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPV 401
           +K         P +T T+DSNPWW+ F     D    L  PEI+ + TD R++R + +P 
Sbjct: 313 QKWM------HPQVTPTDDSNPWWAAFSRVCKDMNLTL-EPEIMPAATDNRYIRAVGVPA 372

Query: 402 LGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           LGFSPM+ TP+LLH+H+E L + VFLRG+ IY  ++ AL+S  A
Sbjct: 373 LGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPA 403

BLAST of Carg24449 vs. TrEMBL
Match: tr|A0A0A0LRI1|A0A0A0LRI1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 PE=4 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 5.9e-212
Identity = 367/449 (81.74%), Postives = 399/449 (88.86%), Query Frame = 0

Query: 1   MANFRLHCRLFS--LAXXXXXXXXXXXXXXXXXXXXXXXXNSPISRFQHYLRIKTAHPDP 60
           M+NF LH RLFS  L                         +SP+SRFQ YLRIKTAHPDP
Sbjct: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSQSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPS 120
           DYASAV+FL SQA EIGLHT+ILEFV GKPLLLVTW GL+PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGLDPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEI 180
           KW++PPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR+R+FVP RTIHISYVPDEEI
Sbjct: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180

Query: 181 GGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSR 240
           GG DGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A+G+PGHGSR
Sbjct: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300
           MYDNSAMENLMKSVEI+++FRESQF++VKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTN 360
           QPSEAEAGFDLR+PPTADPDA+RRRI EEWAPARRN+T QI+EKGPIRDYLG PL+TMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDYLGSPLMTMTN 360

Query: 361 DSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNE 420
            SNPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNE
Sbjct: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLKDTVFLRGIKIYESIISALSSFVADAS 448
           FL D+VF+RGIK+YES+ISALSSF  D S
Sbjct: 421 FLSDSVFIRGIKVYESLISALSSFQEDVS 449

BLAST of Carg24449 vs. TrEMBL
Match: tr|F6H0T0|F6H0T0_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g03230 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 4.1e-197
Identity = 331/404 (81.93%), Postives = 372/404 (92.08%), Query Frame = 0

Query: 40  SPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNP 99
           SPI+RFQ YLRI T HP+PDYASAVSFL+SQAL IGL TK LEF   KPLLL+TW G +P
Sbjct: 43  SPITRFQGYLRINTVHPNPDYASAVSFLKSQALYIGLQTKTLEFARSKPLLLLTWPGSDP 102

Query: 100 SLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRS 159
           SLPSILLNSH+DSVP EPSKWL+PPFSA R+ DGKIFARGSQDDKCIA+QYLEAIRNLR+
Sbjct: 103 SLPSILLNSHLDSVPVEPSKWLHPPFSAYRSPDGKIFARGSQDDKCIAMQYLEAIRNLRA 162

Query: 160 RNFVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYAD 219
           +NF P RTIHISYVPDEEIGGFDGAAKFV SKEF +LNVGFM+DEGQAS GDEFRVFYAD
Sbjct: 163 QNFQPTRTIHISYVPDEEIGGFDGAAKFVASKEFADLNVGFMLDEGQASTGDEFRVFYAD 222

Query: 220 RSPWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISV 279
           RSPW LII+A G PGHGSR+YDNSAMENLMKSVEII+KFRES FD+VKAG+AANSEVISV
Sbjct: 223 RSPWNLIIKAFGMPGHGSRLYDNSAMENLMKSVEIITKFRESLFDVVKAGKAANSEVISV 282

Query: 280 NPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQI 339
           NPV++ AGIPSPTGFVMNMQPSEAEAGFDLR+PPTADPD ++ RI EEWAPA RN+T QI
Sbjct: 283 NPVYLKAGIPSPTGFVMNMQPSEAEAGFDLRMPPTADPDLVKIRIAEEWAPAIRNMTYQI 342

Query: 340 IEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIP 399
           IEKGPIRDY+GRPL+T+TNDSNPWWS+FK AI++AGGKL++PEILASTTDAR+MRQ+ IP
Sbjct: 343 IEKGPIRDYMGRPLMTLTNDSNPWWSIFKQAITEAGGKLAKPEILASTTDARYMRQMGIP 402

Query: 400 VLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFV 444
            LGFSPM+NTPILLH+HNEFLKDT++LRGIK+YES+IS+LSSFV
Sbjct: 403 TLGFSPMTNTPILLHDHNEFLKDTIYLRGIKVYESVISSLSSFV 446

BLAST of Carg24449 vs. TrEMBL
Match: tr|A0A2I4ECL1|A0A2I4ECL1_9ROSI (aminoacylase-1-like OS=Juglans regia OX=51240 GN=LOC108988347 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 2.0e-191
Identity = 317/409 (77.51%), Postives = 366/409 (89.49%), Query Frame = 0

Query: 39  NSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLN 98
           ++PI+RFQ YLRI TAHP PDY+SA+SFLESQAL I L  K LEF PGKP+LL+TW G N
Sbjct: 44  DTPIARFQRYLRINTAHPRPDYSSAISFLESQALSINLQVKTLEFSPGKPVLLLTWPGSN 103

Query: 99  PSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR 158
           PSLPS+L NSH+DSVPAE SKW +PPFSAVR+ DGKIFARG+QDDKCI +QYLEAIRNL+
Sbjct: 104 PSLPSLLFNSHLDSVPAESSKWTHPPFSAVRSPDGKIFARGAQDDKCIGVQYLEAIRNLK 163

Query: 159 SRNFVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYA 218
            +NF P RT+H+SYVPDEEIGG DG  KFV S+EF++LNVGF MDEGQASPGDEFRVFYA
Sbjct: 164 EKNFAPVRTVHVSYVPDEEIGGVDGIFKFVASEEFRKLNVGFAMDEGQASPGDEFRVFYA 223

Query: 219 DRSPWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVIS 278
           DRSPW LII+A+G PGHGSRMYDNSAMENLMKSVEIIS+FRESQFD+VKAGEA NSEVIS
Sbjct: 224 DRSPWNLIIKAKGMPGHGSRMYDNSAMENLMKSVEIISRFRESQFDVVKAGEAPNSEVIS 283

Query: 279 VNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQ 338
           VNPV+V AGIPSP+GFVMNMQPSEAEAGF++R+PP ADP+ +RRRI EEWAPARRN+T Q
Sbjct: 284 VNPVYVKAGIPSPSGFVMNMQPSEAEAGFNIRLPPIADPELVRRRIAEEWAPARRNMTYQ 343

Query: 339 IIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAI 398
           IIE GP+RDY+GRPL+T TNDSNPWWS+FK AI+  GGKL++PEILASTTDAR++RQL I
Sbjct: 344 IIELGPLRDYMGRPLMTPTNDSNPWWSIFKQAITAVGGKLAKPEILASTTDARYIRQLGI 403

Query: 399 PVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADAS 448
           PVLGFSPM NTPILLHEHNEFL+DTV+++GI++YESII +LSSF   +S
Sbjct: 404 PVLGFSPMKNTPILLHEHNEFLEDTVYVKGIEVYESIIRSLSSFEESSS 452

BLAST of Carg24449 vs. TrEMBL
Match: tr|A0A1U8L2P9|A0A1U8L2P9_GOSHI (aminoacylase-1-like OS=Gossypium hirsutum OX=3635 GN=LOC107922227 PE=4 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 3.4e-191
Identity = 315/408 (77.21%), Postives = 362/408 (88.73%), Query Frame = 0

Query: 39  NSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLN 98
           ++PI+RF  YL+  TAHP+P YA  +SFL SQA  IGLH + LEF P KPLLL+TW G N
Sbjct: 22  DTPIARFLRYLQFNTAHPNPSYADPISFLISQANAIGLHARTLEFTPSKPLLLLTWPGSN 81

Query: 99  PSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR 158
           PSLPS+L NSH+DSVPAEPSKW+YPPFSA    DGKI+ARG+QDDKCIA+QYLEAIRNL+
Sbjct: 82  PSLPSVLFNSHLDSVPAEPSKWIYPPFSATLAPDGKIYARGAQDDKCIAMQYLEAIRNLK 141

Query: 159 SRNFVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYA 218
           +  F P RT+HISYVPDEEIGGFDG+AKF  SKEFK+LNVGF++DEGQAS G+EFRVFYA
Sbjct: 142 ANGFTPSRTVHISYVPDEEIGGFDGSAKFAESKEFKDLNVGFVLDEGQASTGEEFRVFYA 201

Query: 219 DRSPWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVIS 278
           DRSPWGLII+A G PGHGSR+YDN AMENLMKSVE+I+KFRESQFD+VK+GEA NSEVIS
Sbjct: 202 DRSPWGLIIKATGDPGHGSRLYDNGAMENLMKSVEVITKFRESQFDIVKSGEAMNSEVIS 261

Query: 279 VNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQ 338
           VNPV++ AGIPSPTGFVMNMQPSEAEAGFDLR+PPTADPD I++RI EEWAPARRN+T +
Sbjct: 262 VNPVYLKAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDLIKKRIAEEWAPARRNMTYE 321

Query: 339 IIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAI 398
           +IEKGPIRDYL RPL+T+TNDSNPWW VFK AI  AGGKLSRPEILASTTDARFMRQ  I
Sbjct: 322 LIEKGPIRDYLDRPLMTLTNDSNPWWPVFKQAIEAAGGKLSRPEILASTTDARFMRQRGI 381

Query: 399 PVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADA 447
           P LGFSPM+NTPILLH+HNEFLKDTV+LRGI++YES+IS+LSSF  ++
Sbjct: 382 PTLGFSPMTNTPILLHDHNEFLKDTVYLRGIEVYESVISSLSSFKGES 429

BLAST of Carg24449 vs. TrEMBL
Match: tr|A0A1U8AX57|A0A1U8AX57_NELNU (aminoacylase-1 OS=Nelumbo nucifera OX=4432 GN=LOC104608485 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 2.2e-190
Identity = 324/405 (80.00%), Postives = 363/405 (89.63%), Query Frame = 0

Query: 39  NSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLN 98
           ++PI+RFQ YLRIKTAHP+PDYASAVSF  S+   IGL T  LEF PGKPLLLVTW G +
Sbjct: 35  DTPITRFQRYLRIKTAHPNPDYASAVSFFTSEVHSIGLKTLTLEFSPGKPLLLVTWPGSD 94

Query: 99  PSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLR 158
           PSLPSILLNSH+DSVPAEPSKWL+PPFSA RTSDGKI+ARGSQDDKCIAIQYLEAIRNLR
Sbjct: 95  PSLPSILLNSHLDSVPAEPSKWLHPPFSATRTSDGKIYARGSQDDKCIAIQYLEAIRNLR 154

Query: 159 -SRNFVPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFY 218
             R F P RT+HISYVPDEEIGGFDGAAKF  SKEF++LN+GFM+DEGQAS  DEFRVFY
Sbjct: 155 MKRKFQPLRTVHISYVPDEEIGGFDGAAKFAASKEFQDLNIGFMLDEGQASTTDEFRVFY 214

Query: 219 ADRSPWGLIIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVI 278
           ADRSPW LII+A G PGHGSRMYDNSAMENLMKS+E IS+FRE+QFD+VKA  AANSEVI
Sbjct: 215 ADRSPWHLIIKAVGMPGHGSRMYDNSAMENLMKSMETISRFRENQFDLVKAKVAANSEVI 274

Query: 279 SVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTS 338
           SVNPV++ AG PSPTGFVMNMQPSEAEAG D+R+PPTADPD +R+RI +EWAPA RN+T 
Sbjct: 275 SVNPVYLKAGTPSPTGFVMNMQPSEAEAGLDVRLPPTADPDLLRKRIADEWAPATRNMTY 334

Query: 339 QIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLA 398
           QI EKGPIRDY+GRPL+T+TN+SNPWWSVFK AI  AGGKLSRPEILASTTDAR+MRQ+ 
Sbjct: 335 QISEKGPIRDYMGRPLMTLTNESNPWWSVFKQAIIAAGGKLSRPEILASTTDARYMRQMG 394

Query: 399 IPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSF 443
           IP LGFSPM NTPILLHEHNE+LKD+VFL+GI++YESIIS+LSSF
Sbjct: 395 IPTLGFSPMINTPILLHEHNEYLKDSVFLKGIEVYESIISSLSSF 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023553812.12.7e-24093.75aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553821.1 aminoacylase-1 [Cucu... [more]
XP_022991055.11.0e-23998.21aminoacylase-1 [Cucurbita maxima][more]
XP_022964785.11.7e-23997.76aminoacylase-1 [Cucurbita moschata][more]
KGN63599.18.9e-21281.74hypothetical protein Csa_1G005600 [Cucumis sativus][more]
XP_022141532.12.0e-21183.00aminoacylase-1 [Momordica charantia] >XP_022141534.1 aminoacylase-1 [Momordica c... [more]
Match NameE-valueIdentityDescription
AT1G44820.13.8e-18070.42Peptidase M20/M25/M40 family protein[more]
AT1G44180.14.3e-17670.20Peptidase M20/M25/M40 family protein[more]
AT4G38220.28.8e-13755.47Peptidase M20/M25/M40 family protein[more]
Match NameE-valueIdentityDescription
sp|Q99JW2|ACY1_MOUSE1.1e-9142.89Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1[more]
sp|P37111|ACY1_PIG3.1e-9141.94Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2[more]
sp|Q6AYS7|ACY1A_RAT1.0e-8942.33Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1[more]
sp|Q5RFB0|ACY1_PONAB3.8e-8942.08Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2[more]
sp|Q03154|ACY1_HUMAN6.5e-8941.58Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LRI1|A0A0A0LRI1_CUCSA5.9e-21281.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 PE=4 SV=1[more]
tr|F6H0T0|F6H0T0_VITVI4.1e-19781.93Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g03230 PE=4 SV=... [more]
tr|A0A2I4ECL1|A0A2I4ECL1_9ROSI2.0e-19177.51aminoacylase-1-like OS=Juglans regia OX=51240 GN=LOC108988347 PE=4 SV=1[more]
tr|A0A1U8L2P9|A0A1U8L2P9_GOSHI3.4e-19177.21aminoacylase-1-like OS=Gossypium hirsutum OX=3635 GN=LOC107922227 PE=4 SV=1[more]
tr|A0A1U8AX57|A0A1U8AX57_NELNU2.2e-19080.00aminoacylase-1 OS=Nelumbo nucifera OX=4432 GN=LOC104608485 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004046aminoacylase activity
GO:0016787hydrolase activity
Vocabulary: Biological Process
TermDefinition
GO:0006520cellular amino acid metabolic process
GO:0008152metabolic process
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: INTERPRO
TermDefinition
IPR036264Bact_exopeptidase_dim_dom
IPR010159N-acyl_aa_amidohydrolase
IPR011650Peptidase_M20_dimer
IPR002933Peptidase_M20
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004046 aminoacylase activity
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24449-RACarg24449-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 105..437
e-value: 5.5E-33
score: 114.3
NoneNo IPR availableGENE3DG3DSA:3.30.70.360coord: 225..331
e-value: 4.0E-9
score: 38.3
NoneNo IPR availableGENE3DG3DSA:3.30.70.1640coord: 361..442
e-value: 3.7E-32
score: 111.8
NoneNo IPR availablePIRSFPIRSF036696ACY-1coord: 31..444
e-value: 1.8E-166
score: 551.5
NoneNo IPR availableGENE3DG3DSA:3.40.630.10coord: 31..224
e-value: 3.3E-54
score: 185.5
NoneNo IPR availablePANTHERPTHR11014:SF56AMINOACYLASE-1coord: 13..443
NoneNo IPR availablePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 13..443
NoneNo IPR availableSUPERFAMILYSSF53187Zn-dependent exopeptidasescoord: 35..220
coord: 355..439
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 221..326
e-value: 2.5E-7
score: 30.5
IPR010159N-acyl-L-amino-acid amidohydrolaseTIGRFAMTIGR01880TIGR01880coord: 36..442
e-value: 2.3E-178
score: 590.9
IPR010159N-acyl-L-amino-acid amidohydrolaseCDDcd05646M20_AcylaseI_likecoord: 39..440
e-value: 0.0
score: 642.793
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILYSSF55031Bacterial exopeptidase dimerisation domaincoord: 225..329