Carg24220 (gene) Silver-seed gourd

BLAST of Carg24220 vs. NCBI nr
Match: XP_022937987.1 (transcription initiation factor TFIID subunit 4b-like [Cucurbita moschata])

HSP 1 Score: 1635.9 bits (4235), Expect = 0.0e+00
Identity = 885/900 (98.33%), Postives = 885/900 (98.33%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 81
           DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE
Sbjct: 14  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 73

Query: 82  SHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQVS 141
           SHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQVS
Sbjct: 74  SHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQVS 133

Query: 142 AHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ 201
           AHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ
Sbjct: 134 AHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ 193

Query: 202 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 261
           VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ
Sbjct: 194 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 253

Query: 262 SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSWY 321
           SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSWY
Sbjct: 254 SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSWY 313

Query: 322 PAKDKNTKSLREVEQRSDGVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMYG 381
           PAKDKNTKSLREVEQRSDGVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMYG
Sbjct: 314 PAKDKNTKSLREVEQRSDGVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMYG 373

Query: 382 NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPHLH 441
           NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPHLH
Sbjct: 374 NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPHLH 433

Query: 442 NNLTAQQVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQVE 501
           NNLTAQQVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQVE
Sbjct: 434 NNLTAQQVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQVE 493

Query: 502 HVKTTPGIGKDALAKQTSKMGFPINNVMPPTSSNAANFISSDASSLHDSNAVLCSQVPSA 561
           HVKTTPGIGKDALAKQTSKMGFPINNVMPPTSSNAANFISSDASSLHDSNAVLCSQVPSA
Sbjct: 494 HVKTTPGIGKDALAKQTSKMGFPINNVMPPTSSNAANFISSDASSLHDSNAVLCSQVPSA 553

Query: 562 ATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVN 621
           ATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVN
Sbjct: 554 ATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVN 613

Query: 622 IEEEEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVE 681
           IEEEEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVE
Sbjct: 614 IEEEEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVE 673

Query: 682 RCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKKR 741
           RCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKKR
Sbjct: 674 RCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKKR 733

Query: 742 GEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDM 801
           GEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDM
Sbjct: 734 GEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDM 793

Query: 802 LSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKATCL 861
           LSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQE            
Sbjct: 794 LSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQE------------ 853

Query: 862 YSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAEATDE 921
              GTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAEATDE
Sbjct: 854 ---GTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAEATDE 898

BLAST of Carg24220 vs. NCBI nr
Match: XP_023538301.1 (transcription initiation factor TFIID subunit 4b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1568.5 bits (4060), Expect = 0.0e+00
Identity = 855/902 (94.79%), Postives = 868/902 (96.23%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 81
           DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE
Sbjct: 14  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 73

Query: 82  SHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQVS 141
           SHVQLD+NFRLKQEQ SSLMEPERCTSVP+NQQQHNAAPLRASKNQPHADREQVEAEQV 
Sbjct: 74  SHVQLDQNFRLKQEQHSSLMEPERCTSVPDNQQQHNAAPLRASKNQPHADREQVEAEQVF 133

Query: 142 AHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ 201
           AHFSQTEELQV+EQVPILMN SNRMQYPDNESQYLKLQKMSNQ+AMVSEQANNSL+RSKQ
Sbjct: 134 AHFSQTEELQVSEQVPILMNNSNRMQYPDNESQYLKLQKMSNQDAMVSEQANNSLSRSKQ 193

Query: 202 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 261
           VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ
Sbjct: 194 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 253

Query: 262 SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSWY 321
           SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFA+LHQKGMNSPAVQSYIPSSASQGQSSSW 
Sbjct: 254 SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFARLHQKGMNSPAVQSYIPSSASQGQSSSWN 313

Query: 322 PAKDKNTKSLREVEQRSDGVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMYG 381
           PA DKN KSLREVEQR DGVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMYG
Sbjct: 314 PAIDKNIKSLREVEQRPDGVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMYG 373

Query: 382 NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPHLH 441
           NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAP+LGRQVTIND KRVQGGNVPHL 
Sbjct: 374 NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPNLGRQVTINDSKRVQGGNVPHLP 433

Query: 442 NNLTAQQ--VSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ 501
           NNLTAQQ  VSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ
Sbjct: 434 NNLTAQQNSVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ 493

Query: 502 VEHVKTTPGIGKDALAKQTSKMGFPINNVMPPTSSNAANFISSDASSLHDSNAVLCSQVP 561
            EHVKTTPGIGKDAL KQTSKMGFPINNVMP TSS+AANFISSDASSLHDSNAVL SQVP
Sbjct: 494 AEHVKTTPGIGKDALDKQTSKMGFPINNVMPLTSSSAANFISSDASSLHDSNAVLSSQVP 553

Query: 562 SAATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG 621
           SAATPGMQNRAPQIK VVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG
Sbjct: 554 SAATPGMQNRAPQIKAVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG 613

Query: 622 VNIEEEEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSNE 681
           VNIEEEE+QLFSSAKENSRAFEASRKVVQEEERLLFQKG LQK LVEI+AKCGLKGM+NE
Sbjct: 614 VNIEEEEQQLFSSAKENSRAFEASRKVVQEEERLLFQKGPLQKMLVEIVAKCGLKGMNNE 673

Query: 682 VERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEK 741
           VERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEK
Sbjct: 674 VERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEK 733

Query: 742 KRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGD 801
           KRGEGEELRRLNDPED QRVAG+KEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGD
Sbjct: 734 KRGEGEELRRLNDPEDDQRVAGDKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGD 793

Query: 802 DMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKAT 861
           DMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQE          
Sbjct: 794 DMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQE---------- 853

Query: 862 CLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAEAT 921
                GTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTT+YRSYNRVRAEAT
Sbjct: 854 -----GTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTMYRSYNRVRAEAT 900

BLAST of Carg24220 vs. NCBI nr
Match: XP_022969558.1 (transcription initiation factor TFIID subunit 4b-like [Cucurbita maxima])

HSP 1 Score: 1476.8 bits (3822), Expect = 0.0e+00
Identity = 818/902 (90.69%), Postives = 830/902 (92.02%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 81
           DETMHSGA VAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE
Sbjct: 14  DETMHSGAVVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 73

Query: 82  SHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQVS 141
           SHVQLD+NFRLKQEQ SSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQ E EQVS
Sbjct: 74  SHVQLDQNFRLKQEQHSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQGEVEQVS 133

Query: 142 AHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ 201
           A FSQTE LQV+EQVPILMN SNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ
Sbjct: 134 AQFSQTEGLQVSEQVPILMNNSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ 193

Query: 202 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 261
           VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ
Sbjct: 194 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 253

Query: 262 SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSWY 321
           SQPPPSVRQPSPRMPSMG GAPNFSDSRPFA+LHQKGMNSPAVQSYIPSSASQGQSSSWY
Sbjct: 254 SQPPPSVRQPSPRMPSMGAGAPNFSDSRPFARLHQKGMNSPAVQSYIPSSASQGQSSSWY 313

Query: 322 PAKDKNTKSLREVEQRSDGVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMYG 381
           PA DKN KS REVEQR DGVNQWTSSSSGTIQERK SSVPVSGLEKQKLHLQQKSFSMYG
Sbjct: 314 PAIDKNIKSSREVEQRPDGVNQWTSSSSGTIQERKGSSVPVSGLEKQKLHLQQKSFSMYG 373

Query: 382 NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPHLH 441
           NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAP+LGRQVTI+D KRVQGGNVPHLH
Sbjct: 374 NSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPNLGRQVTIDDSKRVQGGNVPHLH 433

Query: 442 NNLTAQQ--VSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ 501
           NNLTAQQ  VSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ
Sbjct: 434 NNLTAQQNSVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ 493

Query: 502 VEHVKTTPGIGKDALAKQTSKMGFPINNVMPPTSSNAANFISSDASSLHDSNAVLCSQVP 561
            EHVKTTPGIGKDAL KQTSKMGFPINNVMPPTS+NA N ISSDASSL DSNAVL SQVP
Sbjct: 494 AEHVKTTPGIGKDALEKQTSKMGFPINNVMPPTSANAENLISSDASSLDDSNAVLSSQVP 553

Query: 562 SAATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG 621
           SAATPGMQ+RAPQIK VVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG
Sbjct: 554 SAATPGMQDRAPQIKAVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG 613

Query: 622 VNIEEEEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSNE 681
           VNIEEEEEQLFSSAKENSRAFEASRKVVQEEERLLFQKG LQKKLVEIIAKCGLKGMSNE
Sbjct: 614 VNIEEEEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGPLQKKLVEIIAKCGLKGMSNE 673

Query: 682 VERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEK 741
           VERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTL+TS+VRQQI LANQKAREEWEK
Sbjct: 674 VERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLITSNVRQQIALANQKAREEWEK 733

Query: 742 KRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGD 801
           KRGEGEELRRLNDPEDGQ VA +                           VAARAAFGGD
Sbjct: 734 KRGEGEELRRLNDPEDGQTVAVD-TXXXXXXXXXXXXXXXXXXXXXXXXXVAARAAFGGD 793

Query: 802 DMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKAT 861
           DMLSKWQLMAEQARQKREGRM+SASGSQSGNDAVRKFSSAAGR RKDNQ+          
Sbjct: 794 DMLSKWQLMAEQARQKREGRMDSASGSQSGNDAVRKFSSAAGRCRKDNQK---------- 853

Query: 862 CLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAEAT 921
                GTSRS GSTQINETQTRVARSISVKDLIAVL REPQMCRSTTIYRSYNRVRAEAT
Sbjct: 854 -----GTSRSLGSTQINETQTRVARSISVKDLIAVLLREPQMCRSTTIYRSYNRVRAEAT 899

BLAST of Carg24220 vs. NCBI nr
Match: XP_022952261.1 (transcription initiation factor TFIID subunit 4b isoform X1 [Cucurbita moschata] >XP_022952262.1 transcription initiation factor TFIID subunit 4b isoform X1 [Cucurbita moschata] >XP_022952263.1 transcription initiation factor TFIID subunit 4b isoform X1 [Cucurbita moschata])

HSP 1 Score: 1200.7 bits (3105), Expect = 0.0e+00
Identity = 682/903 (75.53%), Postives = 742/903 (82.17%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 81
           DETMHSGAAV AFQAA NR+IEGDVP VSQ   SD AF  G+NG S L LQA SQN+ TE
Sbjct: 14  DETMHSGAAVEAFQAALNRDIEGDVPAVSQAPESDAAFPRGSNGPSNLSLQA-SQNEKTE 73

Query: 82  SHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQVS 141
           SH Q D+NFR KQEQ SSLME ERC+S+PENQQQHNAAPL+ASKNQP ADREQ + EQVS
Sbjct: 74  SHAQQDQNFRPKQEQHSSLMELERCSSLPENQQQHNAAPLQASKNQPQADREQGDTEQVS 133

Query: 142 AHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ 201
           A FSQT  LQ +E+ PIL+N SNRMQ  DNESQYLKLQKMSNQ+ MVSEQANN LNRSKQ
Sbjct: 134 AQFSQTAGLQGSEKAPILVNDSNRMQNRDNESQYLKLQKMSNQQTMVSEQANNPLNRSKQ 193

Query: 202 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 261
           VPFASLMPVLMPQLDKDR MQLQ+LFN+LKRNEMNKDDF+R +R VVG+QMLRLAV QVQ
Sbjct: 194 VPFASLMPVLMPQLDKDRAMQLQTLFNRLKRNEMNKDDFIRLMRGVVGDQMLRLAVCQVQ 253

Query: 262 SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSWY 321
           +QPPPSVRQ SPRMPSMGPGAPNFSD RPF+QLHQKGMNSPAVQSYIPSSASQG+SSS Y
Sbjct: 254 AQPPPSVRQLSPRMPSMGPGAPNFSDPRPFSQLHQKGMNSPAVQSYIPSSASQGRSSSGY 313

Query: 322 PAKDKNTKSLREVEQRSDG-VNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMY 381
           PA DKN +SLREVEQR+DG  NQ TSSSSGTIQER+RSS+PV GLEKQ+LH QQKSF MY
Sbjct: 314 PAMDKNMQSLREVEQRTDGNANQLTSSSSGTIQERERSSIPVPGLEKQQLHFQQKSFPMY 373

Query: 382 GNSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPHL 441
           GNSGNYHPYTGS+IN SSLS KPQ HEG  KQI QQAP+  RQVTIND KRVQGGNV HL
Sbjct: 374 GNSGNYHPYTGSNINTSSLSLKPQPHEGQAKQISQQAPNFDRQVTINDPKRVQGGNVSHL 433

Query: 442 HNNLTAQQVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQV 501
            NNLT+Q   WKSS SKEQNTGPLSSMSYIKQ+ S QVSEQNK Q SNLQGLSS+ SMQ 
Sbjct: 434 RNNLTSQPSPWKSSTSKEQNTGPLSSMSYIKQEPSDQVSEQNKTQHSNLQGLSSIPSMQA 493

Query: 502 EHVKTTPGIGKDALAKQTSKMGFPI-NNVMPPTSSNAANFISSDASSLHDSNAVLCSQVP 561
           E V TTPGI KD   KQTSK+ FP  NNVMP +S+NAAN ISSDA+SLH+S+AVL SQVP
Sbjct: 494 EQVNTTPGIAKDPFEKQTSKVAFPTSNNVMPQSSTNAANPISSDANSLHESSAVLSSQVP 553

Query: 562 SAATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG 621
           SA TPGMQNRAPQ K  VGQKKPLE LGSSPPL SKKQK+SGAFSDQSIEQLNDVT VSG
Sbjct: 554 SATTPGMQNRAPQKKGAVGQKKPLEALGSSPPLSSKKQKVSGAFSDQSIEQLNDVTAVSG 613

Query: 622 VNIEE-EEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSN 681
           VNI E                                    LQKKL+EI+AK GLK MSN
Sbjct: 614 VNIREXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPLQKKLMEIMAKYGLKSMSN 673

Query: 682 EVERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWE 741
           +VE+CLSL VEERLRGIISNLIRLSKQRVD EKPRHRT +TSDVRQQI L NQKAREEWE
Sbjct: 674 DVEKCLSLSVEERLRGIISNLIRLSKQRVDTEKPRHRTHITSDVRQQIMLVNQKAREEWE 733

Query: 742 KKRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGG 801
           KK+ E E+LR+LNDPEDG  +AG+K+KDEGRMKSVK NKEEDDKMRTTAANVAARAA GG
Sbjct: 734 KKQAEEEKLRKLNDPEDGSDIAGDKDKDEGRMKSVKVNKEEDDKMRTTAANVAARAAVGG 793

Query: 802 DDMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKA 861
           DDMLSKWQLMAEQARQKREG M+SASGSQSG D VRK SSAAGR  KDNQEG+++     
Sbjct: 794 DDMLSKWQLMAEQARQKREGGMDSASGSQSGKDTVRKSSSAAGRHGKDNQEGDRK----- 853

Query: 862 TCLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAEA 921
                 GTS+ FG  Q++ +QTRVARSISVKD+IAVLQREPQM RSTTIYR +N+VR+EA
Sbjct: 854 ------GTSKKFGRNQMSASQTRVARSISVKDVIAVLQREPQMSRSTTIYRLFNKVRSEA 904

BLAST of Carg24220 vs. NCBI nr
Match: XP_023554163.1 (transcription initiation factor TFIID subunit 4b isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554164.1 transcription initiation factor TFIID subunit 4b isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554165.1 transcription initiation factor TFIID subunit 4b isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 682/903 (75.53%), Postives = 742/903 (82.17%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 81
           DETMHSGAAV AFQAA NR+IEGDVP VSQ   SD AF  G+NG S L LQA SQN+ TE
Sbjct: 14  DETMHSGAAVEAFQAALNRDIEGDVPAVSQAPESDAAFPRGSNGPSSLSLQA-SQNEKTE 73

Query: 82  SHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQVS 141
           SH Q D+NFR KQEQ SSLME ERC+S+PENQQQHNAAPL+ASKNQP ADREQ +AEQVS
Sbjct: 74  SHAQQDQNFRPKQEQHSSLMELERCSSLPENQQQHNAAPLQASKNQPQADREQGDAEQVS 133

Query: 142 AHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSKQ 201
           A FSQT  LQ +E+ PIL+N SNRMQ  DNESQYLKLQKMSNQ+ MVSEQANN LNRSKQ
Sbjct: 134 AQFSQTAGLQGSEKAPILVNDSNRMQNRDNESQYLKLQKMSNQQTMVSEQANNPLNRSKQ 193

Query: 202 VPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQ 261
           VPFASLMPVLMPQLDKDR MQLQ+LFN+LKRNEMNKDDF+R +R VVG+QMLRLAV QVQ
Sbjct: 194 VPFASLMPVLMPQLDKDRAMQLQTLFNRLKRNEMNKDDFIRLMRGVVGDQMLRLAVCQVQ 253

Query: 262 SQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSWY 321
           +QPPPSVRQ SPRMPSMGPGAPNFSD RPF+QLHQKGMNSPAVQSY+PSSASQG+ SS Y
Sbjct: 254 AQPPPSVRQLSPRMPSMGPGAPNFSDPRPFSQLHQKGMNSPAVQSYMPSSASQGRGSSGY 313

Query: 322 PAKDKNTKSLREVEQRSDG-VNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSMY 381
           PA DKN +SLREVEQR+DG  NQ TSSSSGTIQER+RSS+PV GLEKQ+LH QQKSF MY
Sbjct: 314 PAMDKNMQSLREVEQRTDGNANQLTSSSSGTIQERERSSIPVPGLEKQQLHFQQKSFPMY 373

Query: 382 GNSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPHL 441
           GNSGNYHPYTGS+IN SSLS KPQ HEG  KQI QQAP+  RQV+IND KRVQGGNV HL
Sbjct: 374 GNSGNYHPYTGSNINTSSLSLKPQPHEGQAKQISQQAPNFDRQVSINDPKRVQGGNVSHL 433

Query: 442 HNNLTAQQVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQV 501
            NNLT+Q   WKSS SKEQNTGPLSSMSYIKQ+ S QVSEQNK Q SNLQGLSS+ SMQ 
Sbjct: 434 RNNLTSQPSPWKSSTSKEQNTGPLSSMSYIKQEPSDQVSEQNKTQHSNLQGLSSIPSMQA 493

Query: 502 EHVKTTPGIGKDALAKQTSKMGFPI-NNVMPPTSSNAANFISSDASSLHDSNAVLCSQVP 561
           E V TTPGI KD   KQTSK+ FP  NNVMP +S+NAAN ISSDA+SLH+S+AVL SQVP
Sbjct: 494 EQVNTTPGIAKDPFEKQTSKVAFPTSNNVMPQSSTNAANPISSDANSLHESSAVLSSQVP 553

Query: 562 SAATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSG 621
           SA TPGMQNRAPQ K  VGQKKPLE LGSSPPL SKKQK+SGAFSDQSIEQLNDVT VSG
Sbjct: 554 SATTPGMQNRAPQKKGAVGQKKPLEALGSSPPLSSKKQKVSGAFSDQSIEQLNDVTAVSG 613

Query: 622 VNIEE-EEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSN 681
           VNI E                                    LQKKL+EI+AK GLK MSN
Sbjct: 614 VNIREXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPLQKKLMEIMAKYGLKSMSN 673

Query: 682 EVERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWE 741
           +VE+CLSL VEERLRGIISNLIRLSKQRVD EKPRHRT +TSDVRQQI L NQKAREEWE
Sbjct: 674 DVEKCLSLSVEERLRGIISNLIRLSKQRVDTEKPRHRTHITSDVRQQIMLVNQKAREEWE 733

Query: 742 KKRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGG 801
           KK+ E E+LR+LNDPEDG  VAG+K+KDEGRMKSVK NKEEDDKMRTTAANVAARAA GG
Sbjct: 734 KKQAEEEKLRKLNDPEDGSDVAGDKDKDEGRMKSVKVNKEEDDKMRTTAANVAARAAVGG 793

Query: 802 DDMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKA 861
           DDMLSKWQLMAEQARQKREG M+SASGSQSG D VRK SSAAGR  KDNQEG+++     
Sbjct: 794 DDMLSKWQLMAEQARQKREGGMDSASGSQSGKDTVRKSSSAAGRHGKDNQEGDRK----- 853

Query: 862 TCLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAEA 921
                 GTS+ FG  Q+N +QTRVARSISVKD+IAVLQREPQM RSTTIYR +N+VR+EA
Sbjct: 854 ------GTSKKFGRNQMNASQTRVARSISVKDVIAVLQREPQMSRSTTIYRLFNKVRSEA 904

BLAST of Carg24220 vs. TAIR10
Match: AT5G43130.2 (TBP-associated factor 4)

HSP 1 Score: 447.2 bits (1149), Expect = 2.4e-125
Identity = 378/949 (39.83%), Postives = 501/949 (52.79%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 81
           DE+MHSGA V AFQAA NR+IEG     S T +     + GNN S       S Q  +T 
Sbjct: 14  DESMHSGADVDAFQAALNRDIEG-----SMTTSIPHVTNPGNNHS-------SRQQFSTW 73

Query: 82  SHVQLDKNFRLK-QEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVE---- 141
            +   D N  ++ Q    S    E+  S  ENQ QH+        N+PH    Q +    
Sbjct: 74  KNGIGDSNINVQTQHSLESTQMKEQEGSTLENQHQHDL----KRANEPHLQHNQPQDLHR 133

Query: 142 AEQVSAHFSQTEE---LQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQAN 201
           A Q+  + SQ  +   L ++E+ P   N S+R    ++ESQY+KLQKMS+Q+A   E   
Sbjct: 134 AGQLWENPSQVPQSTGLPISEKNP-TGNESDRSHNQESESQYMKLQKMSSQQARGVEPPV 193

Query: 202 NSL------NRSKQVPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSV 261
           N +         KQVPFA+L+P LM QLDKDR +QL++L+ +LK+NE+ K+ F R ++ +
Sbjct: 194 NPMXXXXXXXXXKQVPFAALLPTLMNQLDKDRALQLRTLYARLKKNEIPKEGFTRHMKDI 253

Query: 262 VGNQMLRLAVSQVQSQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSY 321
           VG+QMLR+AVS++Q                                  Q   N   +   
Sbjct: 254 VGDQMLRMAVSKLQ----------------------------------QVNYNQGKIGIQ 313

Query: 322 IPSSASQGQSSSWYPAKDKNTKSLREVEQRSDGVNQWTSSSSGTIQERKRSSVPVSGLEK 381
            PS+    Q S              + + R+  +NQ  SS+SGT+     SSVPV GL K
Sbjct: 314 APSTEINNQKS--------------QSDPRAVHLNQLPSSASGTL----GSSVPVQGLTK 373

Query: 382 QKLHLQQ---KSFSMYGNSGNYHPYTGSSINASSLSSKPQTHEGHVKQI----------- 441
              H  Q    SF MY  SG++H + G + NAS  + +P  H+ H++ +           
Sbjct: 374 HPQHQMQHPPSSFPMYTTSGSFHSFPGPNTNASGSTLRPHLHDSHMRHVAHNQPMGSTGL 433

Query: 442 ---PQQA------PSLGRQVTINDFKRVQGGNVPHLHNNLTAQQVSWKSSISKEQNTGPL 501
              PQ        P   R  ++ND  RVQGG   H  N+         SS+      G  
Sbjct: 434 GGPPQSTTNMMTMPKFERPSSVNDPSRVQGGATSHFQNS---------SSLPLNSAPGQG 493

Query: 502 SSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQVEHVKTTPGIGKDALAKQTSKMGFP 561
           SS+S++KQ+S  Q  E+N     N   ++S                 + L K++S+M   
Sbjct: 494 SSVSHVKQESVDQSFEKN-----NAASMTS----------------NEDLEKESSRMVLS 553

Query: 562 INNVMPPTSSNAANFISSDASSLHDSNAVLCSQVPSAATPGMQN-RAPQIKVVVGQKKPL 621
             N M P SS     +S   ++  D++  + S+ P   + G  N R P  K  VGQKKPL
Sbjct: 554 TPNNMAPASS-----VSPSMTTQLDASTTMNSRGPLGTSQGGANARMPPKKPSVGQKKPL 613

Query: 622 ETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVNIEEEEEQLFSSAKENSRAFEAS 681
           ETLGSS        K++G   DQSIEQLNDVT VSGVN+ EEEEQLFS AKE+ R  EAS
Sbjct: 614 ETLGSSXXXXXXXXKVAGNSMDQSIEQLNDVTAVSGVNLREEEEQLFSGAKEDGRVSEAS 673

Query: 682 RKVV-QEEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVERCLSLCVEERLRGIISNLIRL 741
           R+VV +EEERL+ QK  LQ+KL EI+AK GLK +SN+VERCLSLCVEER+RG++S++IRL
Sbjct: 674 RRVVHEEEERLILQKNPLQRKLAEIMAKAGLKQISNDVERCLSLCVEERMRGLLSHIIRL 733

Query: 742 SKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKK-RGEGEELRRLNDPEDGQRVAG 801
           SKQRVDAEK RHRT +TSD+R QI   NQK +EEWEKK                      
Sbjct: 734 SKQRVDAEKSRHRTFITSDIRLQINEMNQKVKEEWEKKXXXXXXXXXXXXXXXXXXXXXX 793

Query: 802 EKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDMLSKWQLMAEQARQKREGRMN 861
                + R K VK NKE+DDKMRTTAANVAARAA GGDD   KWQLMAE ARQK      
Sbjct: 794 XXXXXDNRSKGVKGNKEDDDKMRTTAANVAARAAVGGDDAFLKWQLMAE-ARQK------ 849

Query: 862 SASGSQSGNDAVRKFSSAAGRRRKDNQEGEKR-SHLKATC----LYSPGTSR------SF 910
             S S++G D  +K +S      KD Q+G +R S  +++C    +Y   +SR      SF
Sbjct: 854 --SVSEAGKDGNQKTTSXXXXNSKDRQDGGRRFSGTESSCGVGIVYRVSSSRFWFAMMSF 849

BLAST of Carg24220 vs. TAIR10
Match: AT1G27720.1 (TBP-associated factor 4B)

HSP 1 Score: 291.6 bits (745), Expect = 1.7e-78
Identity = 269/776 (34.66%), Postives = 383/776 (49.36%), Query Frame = 0

Query: 172 ESQYLKLQKMSNQEAMVSEQANNSLNRSKQVPFASLMPVLMPQLDKDRGMQLQ--SLFNK 231
           + +Y+KLQKMS++E    E+  + +N + ++  A +  +L   +D   G +    +L  K
Sbjct: 55  QPRYMKLQKMSSKETPWVEKTVDPVNHNLRL--ARVTDLLRTVVDHQPGKKTHCLNLHYK 114

Query: 232 LKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQSQPPPSVRQPSPRMPSMGPGAPNFSDSR 291
           LKR E+  ++F+R +R +VG+Q++R  +SQ               +P + PG        
Sbjct: 115 LKRKELTMEEFMRQLRDLVGDQIIRSVISQ---------------LPQLKPG-------- 174

Query: 292 PFAQLHQKGMNSPAVQSYIPSSASQGQSSSWYPAKDKNTKSLREVEQRSD----GVNQWT 351
                   G+  P            G+S+      DK +KS     Q SD     VNQ +
Sbjct: 175 ------NMGIKVP------------GRSN-----HDKVSKSAEFTAQESDPREVHVNQLS 234

Query: 352 SSSSGTIQERKRSSVPVSGLEK---QKLHLQQKSFSMYGNSGNYHPYTGSSINASSLSSK 411
           S++SGT+     SS  V GL K   Q + L   SF M   SG+ +PY G+++ +   SS+
Sbjct: 235 STTSGTL----NSSTTVQGLNKHPEQHMQLPSSSFHMDTKSGSLNPYPGTNVTSPGSSSR 294

Query: 412 PQTHEGHVKQIPQQ------------------APSLGRQVTINDFKRVQGGNVPHLHNNL 471
            +  +   ++  Q                    P   R   +N   RVQ G +     N 
Sbjct: 295 AKLPDFQHRENNQNVGIASVGGPTKSTINMTTVPKFERPTFVNGPSRVQDGPISDFPKNS 354

Query: 472 TAQQVS--WKSSISKEQNTGPLSSMSYIKQQSSGQVSEQ-NKPQLSNLQGLSSVSSMQVE 531
           +    S  W+ S++K+   GP SS+ +++ +   Q  EQ +KP+    QG+++V   Q  
Sbjct: 355 SFPLYSAPWQGSVTKDHTVGPSSSVIHVEHKLIDQSFEQAHKPRYLVQQGVTNVPLKQKN 414

Query: 532 HVKTTPGIGKDALAKQTSKMGFPINNVMPPTSSNAANFISSDASSLHDSNAVLCSQVPSA 591
            +   P    D L KQ+SKMG         TS+ +A+ +    ++  DS+ ++    PS 
Sbjct: 415 AI---PISSNDDLEKQSSKMGL-------FTSTTSASSVFPSMTTQLDSSTMVNMPAPSE 474

Query: 592 ATPGMQNRAPQIKV-VVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGV 651
             P + N     K+  VGQKKPLE LGSS P   KKQK+ G  SD+SIE+ NDVT VSG+
Sbjct: 475 TIPKIANVTVTPKMPSVGQKKPLEALGSSLPPSRKKQKICGTSSDESIEKFNDVTAVSGI 534

Query: 652 NIEEEEEQLFSSA-KENSRAFEASRKVVQ-EEERLLFQKGQLQKKLVEIIAKCGLKGMSN 711
           N+ EEE+QL  S  K+N R  +A R++V  EEER L QK  LQ+KL EI+ K GLK + +
Sbjct: 535 NLREEEKQLLDSGPKKNDRVSKAYRRLVHGEEERTLLQKIPLQRKLTEIMGKSGLKHIDH 594

Query: 712 EVERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWE 771
           +VERCLSLCVEER+RG++ N+IR+SKQR DAEK R+RT +TSD+R++I   NQK +EEWE
Sbjct: 595 DVERCLSLCVEERMRGLLFNIIRISKQRTDAEKCRNRTFITSDIRKEINEMNQKVKEEWE 654

Query: 772 KKRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGG 831
           KK                                               ANVA RAA GG
Sbjct: 655 KKHS-----------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXANVAVRAAVGG 714

Query: 832 DDMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKA 891
           DD  SKW+LMAE ARQ+                     S   GR  K             
Sbjct: 715 DDRFSKWKLMAE-ARQRS--------------------SPGPGRNSKK------------ 716

Query: 892 TCLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRV 915
               S GT   FG    N+   +V RSISVKD+IAV+++EPQM RST +YR YNR+
Sbjct: 775 ---LSGGT--QFGK---NQGLPKVVRSISVKDVIAVVEKEPQMSRSTLLYRVYNRI 716

BLAST of Carg24220 vs. Swiss-Prot
Match: sp|F4K4L7|TAF4B_ARATH (Transcription initiation factor TFIID subunit 4b OS=Arabidopsis thaliana OX=3702 GN=TAF4B PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.4e-124
Identity = 378/949 (39.83%), Postives = 501/949 (52.79%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAFSGGNNGSSGLLLQASSQNKNTE 81
           DE+MHSGA V AFQAA NR+IEG     S T +     + GNN S       S Q  +T 
Sbjct: 14  DESMHSGADVDAFQAALNRDIEG-----SMTTSIPHVTNPGNNHS-------SRQQFSTW 73

Query: 82  SHVQLDKNFRLK-QEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVE---- 141
            +   D N  ++ Q    S    E+  S  ENQ QH+        N+PH    Q +    
Sbjct: 74  KNGIGDSNINVQTQHSLESTQMKEQEGSTLENQHQHDL----KRANEPHLQHNQPQDLHR 133

Query: 142 AEQVSAHFSQTEE---LQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQAN 201
           A Q+  + SQ  +   L ++E+ P   N S+R    ++ESQY+KLQKMS+Q+A   E   
Sbjct: 134 AGQLWENPSQVPQSTGLPISEKNP-TGNESDRSHNQESESQYMKLQKMSSQQARGVEPPV 193

Query: 202 NSL------NRSKQVPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSV 261
           N +         KQVPFA+L+P LM QLDKDR +QL++L+ +LK+NE+ K+ F R ++ +
Sbjct: 194 NPMXXXXXXXXXKQVPFAALLPTLMNQLDKDRALQLRTLYARLKKNEIPKEGFTRHMKDI 253

Query: 262 VGNQMLRLAVSQVQSQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSY 321
           VG+QMLR+AVS++Q                                  Q   N   +   
Sbjct: 254 VGDQMLRMAVSKLQ----------------------------------QVNYNQGKIGIQ 313

Query: 322 IPSSASQGQSSSWYPAKDKNTKSLREVEQRSDGVNQWTSSSSGTIQERKRSSVPVSGLEK 381
            PS+    Q S              + + R+  +NQ  SS+SGT+     SSVPV GL K
Sbjct: 314 APSTEINNQKS--------------QSDPRAVHLNQLPSSASGTL----GSSVPVQGLTK 373

Query: 382 QKLHLQQ---KSFSMYGNSGNYHPYTGSSINASSLSSKPQTHEGHVKQI----------- 441
              H  Q    SF MY  SG++H + G + NAS  + +P  H+ H++ +           
Sbjct: 374 HPQHQMQHPPSSFPMYTTSGSFHSFPGPNTNASGSTLRPHLHDSHMRHVAHNQPMGSTGL 433

Query: 442 ---PQQA------PSLGRQVTINDFKRVQGGNVPHLHNNLTAQQVSWKSSISKEQNTGPL 501
              PQ        P   R  ++ND  RVQGG   H  N+         SS+      G  
Sbjct: 434 GGPPQSTTNMMTMPKFERPSSVNDPSRVQGGATSHFQNS---------SSLPLNSAPGQG 493

Query: 502 SSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQVEHVKTTPGIGKDALAKQTSKMGFP 561
           SS+S++KQ+S  Q  E+N     N   ++S                 + L K++S+M   
Sbjct: 494 SSVSHVKQESVDQSFEKN-----NAASMTS----------------NEDLEKESSRMVLS 553

Query: 562 INNVMPPTSSNAANFISSDASSLHDSNAVLCSQVPSAATPGMQN-RAPQIKVVVGQKKPL 621
             N M P SS     +S   ++  D++  + S+ P   + G  N R P  K  VGQKKPL
Sbjct: 554 TPNNMAPASS-----VSPSMTTQLDASTTMNSRGPLGTSQGGANARMPPKKPSVGQKKPL 613

Query: 622 ETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVNIEEEEEQLFSSAKENSRAFEAS 681
           ETLGSS        K++G   DQSIEQLNDVT VSGVN+ EEEEQLFS AKE+ R  EAS
Sbjct: 614 ETLGSSXXXXXXXXKVAGNSMDQSIEQLNDVTAVSGVNLREEEEQLFSGAKEDGRVSEAS 673

Query: 682 RKVV-QEEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVERCLSLCVEERLRGIISNLIRL 741
           R+VV +EEERL+ QK  LQ+KL EI+AK GLK +SN+VERCLSLCVEER+RG++S++IRL
Sbjct: 674 RRVVHEEEERLILQKNPLQRKLAEIMAKAGLKQISNDVERCLSLCVEERMRGLLSHIIRL 733

Query: 742 SKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKK-RGEGEELRRLNDPEDGQRVAG 801
           SKQRVDAEK RHRT +TSD+R QI   NQK +EEWEKK                      
Sbjct: 734 SKQRVDAEKSRHRTFITSDIRLQINEMNQKVKEEWEKKXXXXXXXXXXXXXXXXXXXXXX 793

Query: 802 EKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDMLSKWQLMAEQARQKREGRMN 861
                + R K VK NKE+DDKMRTTAANVAARAA GGDD   KWQLMAE ARQK      
Sbjct: 794 XXXXXDNRSKGVKGNKEDDDKMRTTAANVAARAAVGGDDAFLKWQLMAE-ARQK------ 849

Query: 862 SASGSQSGNDAVRKFSSAAGRRRKDNQEGEKR-SHLKATC----LYSPGTSR------SF 910
             S S++G D  +K +S      KD Q+G +R S  +++C    +Y   +SR      SF
Sbjct: 854 --SVSEAGKDGNQKTTSXXXXNSKDRQDGGRRFSGTESSCGVGIVYRVSSSRFWFAMMSF 849

BLAST of Carg24220 vs. Swiss-Prot
Match: sp|Q6SJR1|TAF4_ARATH (Transcription initiation factor TFIID subunit 4 OS=Arabidopsis thaliana OX=3702 GN=TAF4 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 3.1e-77
Identity = 269/776 (34.66%), Postives = 383/776 (49.36%), Query Frame = 0

Query: 172 ESQYLKLQKMSNQEAMVSEQANNSLNRSKQVPFASLMPVLMPQLDKDRGMQLQ--SLFNK 231
           + +Y+KLQKMS++E    E+  + +N + ++  A +  +L   +D   G +    +L  K
Sbjct: 55  QPRYMKLQKMSSKETPWVEKTVDPVNHNLRL--ARVTDLLRTVVDHQPGKKTHCLNLHYK 114

Query: 232 LKRNEMNKDDFVRCIRSVVGNQMLRLAVSQVQSQPPPSVRQPSPRMPSMGPGAPNFSDSR 291
           LKR E+  ++F+R +R +VG+Q++R  +SQ               +P + PG        
Sbjct: 115 LKRKELTMEEFMRQLRDLVGDQIIRSVISQ---------------LPQLKPG-------- 174

Query: 292 PFAQLHQKGMNSPAVQSYIPSSASQGQSSSWYPAKDKNTKSLREVEQRSD----GVNQWT 351
                   G+  P            G+S+      DK +KS     Q SD     VNQ +
Sbjct: 175 ------NMGIKVP------------GRSN-----HDKVSKSAEFTAQESDPREVHVNQLS 234

Query: 352 SSSSGTIQERKRSSVPVSGLEK---QKLHLQQKSFSMYGNSGNYHPYTGSSINASSLSSK 411
           S++SGT+     SS  V GL K   Q + L   SF M   SG+ +PY G+++ +   SS+
Sbjct: 235 STTSGTL----NSSTTVQGLNKHPEQHMQLPSSSFHMDTKSGSLNPYPGTNVTSPGSSSR 294

Query: 412 PQTHEGHVKQIPQQ------------------APSLGRQVTINDFKRVQGGNVPHLHNNL 471
            +  +   ++  Q                    P   R   +N   RVQ G +     N 
Sbjct: 295 AKLPDFQHRENNQNVGIASVGGPTKSTINMTTVPKFERPTFVNGPSRVQDGPISDFPKNS 354

Query: 472 TAQQVS--WKSSISKEQNTGPLSSMSYIKQQSSGQVSEQ-NKPQLSNLQGLSSVSSMQVE 531
           +    S  W+ S++K+   GP SS+ +++ +   Q  EQ +KP+    QG+++V   Q  
Sbjct: 355 SFPLYSAPWQGSVTKDHTVGPSSSVIHVEHKLIDQSFEQAHKPRYLVQQGVTNVPLKQKN 414

Query: 532 HVKTTPGIGKDALAKQTSKMGFPINNVMPPTSSNAANFISSDASSLHDSNAVLCSQVPSA 591
            +   P    D L KQ+SKMG         TS+ +A+ +    ++  DS+ ++    PS 
Sbjct: 415 AI---PISSNDDLEKQSSKMGL-------FTSTTSASSVFPSMTTQLDSSTMVNMPAPSE 474

Query: 592 ATPGMQNRAPQIKV-VVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGV 651
             P + N     K+  VGQKKPLE LGSS P   KKQK+ G  SD+SIE+ NDVT VSG+
Sbjct: 475 TIPKIANVTVTPKMPSVGQKKPLEALGSSLPPSRKKQKICGTSSDESIEKFNDVTAVSGI 534

Query: 652 NIEEEEEQLFSSA-KENSRAFEASRKVVQ-EEERLLFQKGQLQKKLVEIIAKCGLKGMSN 711
           N+ EEE+QL  S  K+N R  +A R++V  EEER L QK  LQ+KL EI+ K GLK + +
Sbjct: 535 NLREEEKQLLDSGPKKNDRVSKAYRRLVHGEEERTLLQKIPLQRKLTEIMGKSGLKHIDH 594

Query: 712 EVERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWE 771
           +VERCLSLCVEER+RG++ N+IR+SKQR DAEK R+RT +TSD+R++I   NQK +EEWE
Sbjct: 595 DVERCLSLCVEERMRGLLFNIIRISKQRTDAEKCRNRTFITSDIRKEINEMNQKVKEEWE 654

Query: 772 KKRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGG 831
           KK                                               ANVA RAA GG
Sbjct: 655 KKHS-----------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXANVAVRAAVGG 714

Query: 832 DDMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKA 891
           DD  SKW+LMAE ARQ+                     S   GR  K             
Sbjct: 715 DDRFSKWKLMAE-ARQRS--------------------SPGPGRNSKK------------ 716

Query: 892 TCLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRV 915
               S GT   FG    N+   +V RSISVKD+IAV+++EPQM RST +YR YNR+
Sbjct: 775 ---LSGGT--QFGK---NQGLPKVVRSISVKDVIAVVEKEPQMSRSTLLYRVYNRI 716

BLAST of Carg24220 vs. Swiss-Prot
Match: sp|Q92750|TAF4B_HUMAN (Transcription initiation factor TFIID subunit 4B OS=Homo sapiens OX=9606 GN=TAF4B PE=1 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 6.0e-04
Identity = 55/214 (25.70%), Postives = 108/214 (50.47%), Query Frame = 0

Query: 585 TLGSSPPLPSK-KQKLSGAFSDQSIEQLNDVTVVSGVNIEEEEEQLFSSAKENSRAFEAS 644
           +L +SP   ++ K+ ++  F D+  + +NDVT ++GVN+ EE   + ++  E       S
Sbjct: 591 SLQASPTQKNRIKENVTSCFRDE--DDINDVTSMAGVNLNEENACILATNSELVGTLIQS 650

Query: 645 RKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVERCLSLCVEERLRGIISNLIRLS 704
            K    +E  LF  G LQK++++I  K  +  ++++    +S   +ERLRG++  L  ++
Sbjct: 651 CK----DEPFLF-IGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIA 710

Query: 705 KQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKKRGEGEELRRLNDPEDGQRVAGEK 764
           + R+   K     ++ SD R Q+    +   ++ EK+R + EE   L      +    + 
Sbjct: 711 QHRMTTYKASENYILCSDTRSQLKFLEK--LDQLEKQRKDLEEREMLLKAAKSRSNKEDP 770

Query: 765 EKDEGRMKSVKANKEEDDKMRTTAANVAARAAFG 798
           E+   + K+ +  + E  +++   AN+ A AA G
Sbjct: 771 EQLRLKQKAKELQQLELAQIQHRDANLTALAAIG 795

BLAST of Carg24220 vs. TrEMBL
Match: tr|A0A1S3CQ23|A0A1S3CQ23_CUCME (transcription initiation factor TFIID subunit 4b OS=Cucumis melo OX=3656 GN=LOC103503522 PE=4 SV=1)

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 662/904 (73.23%), Postives = 723/904 (79.98%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAF-SGGNNGSSGLLLQASSQNKNT 81
           DE+MHSGAAV AFQAA NR+IEGD   VSQT+ SD AF  G NNGSS L LQASSQ++NT
Sbjct: 14  DESMHSGAAVDAFQAALNRDIEGDAQAVSQTSESDAAFPQGNNNGSSTLSLQASSQSENT 73

Query: 82  ESHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQV 141
           ESHVQ ++NFRLKQEQ SSLME +R  SVPENQQQH++AP + SKNQP ADREQ E EQV
Sbjct: 74  ESHVQQNQNFRLKQEQHSSLMELDR--SVPENQQQHSSAPFQVSKNQPQADREQGEGEQV 133

Query: 142 SAHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSK 201
           SA FSQT  LQV+E+ PIL+N SNRMQ  DNESQYLKLQKMSNQ+++V+EQANN LNRSK
Sbjct: 134 SAQFSQTAGLQVSEKAPILVNDSNRMQNRDNESQYLKLQKMSNQQSIVAEQANNPLNRSK 193

Query: 202 QVPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQV 261
           QVPFASLMPVLMPQLDKDRGMQLQ+LFN+LKRNEMNKDDF+R +R VVG+QMLRLAV QV
Sbjct: 194 QVPFASLMPVLMPQLDKDRGMQLQTLFNRLKRNEMNKDDFIRLMRGVVGDQMLRLAVCQV 253

Query: 262 QSQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSW 321
           QSQPPPSVRQ SPRMPSMGPG PNFSD RPF QLH KGMN PAVQSYIPS ASQG+SSS 
Sbjct: 254 QSQPPPSVRQLSPRMPSMGPGTPNFSDPRPFTQLHPKGMNPPAVQSYIPSPASQGRSSSG 313

Query: 322 YPAKDKNTKSLREVEQRSDG-VNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSM 381
           YPA +KN +SLREVEQR DG  NQ TSSS+ TIQ+R+RSSV V GLEKQ+LH QQKSF M
Sbjct: 314 YPAMEKNMQSLREVEQRPDGNGNQITSSSTSTIQDRERSSVSVPGLEKQQLHFQQKSFPM 373

Query: 382 YGNSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPH 441
           YGNSGNYH YTGS+INASSLS KPQ HEG VKQI QQA +  RQVTIND KRVQGG+VPH
Sbjct: 374 YGNSGNYHTYTGSNINASSLSLKPQPHEGQVKQISQQASNFDRQVTINDSKRVQGGSVPH 433

Query: 442 LHNNLTAQQVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ 501
           LHNNLT+QQ  WKSS SKEQN      MSY+KQ+ S QVSEQNK Q SNLQGLSS+ SMQ
Sbjct: 434 LHNNLTSQQNPWKSSTSKEQNI-----MSYVKQEPSDQVSEQNKTQHSNLQGLSSIPSMQ 493

Query: 502 VEHVKTTPGIGKDALAKQTSKMGFPI-NNVMPPTSSNAANFISSDASSLHDSNAVLCSQV 561
            EHV TTPGI KD   KQTSKMGFP  NNV+P                  ++NAVL SQV
Sbjct: 494 AEHVNTTPGIAKDPFDKQTSKMGFPTSNNVVPXXXXXXXXXXXXXXXXXXETNAVLNSQV 553

Query: 562 PSAATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVS 621
           PSA TPGMQNRAPQ K  VGQKKPLE LGSSPPL SKKQK+SGAF+DQSIEQLNDVT VS
Sbjct: 554 PSATTPGMQNRAPQKKAAVGQKKPLEALGSSPPLSSKKQKVSGAFADQSIEQLNDVTAVS 613

Query: 622 GVNIEE-EEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMS 681
           GVNI E                                    LQKKLVEI+AKCGLKGMS
Sbjct: 614 GVNIREXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPLQKKLVEIMAKCGLKGMS 673

Query: 682 NEVERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEW 741
           N+VE+CLSLCVEERLRG+ISNLIRLSKQRVDAEKPRHRT++TSDVRQQITL NQKAREEW
Sbjct: 674 NDVEKCLSLCVEERLRGVISNLIRLSKQRVDAEKPRHRTVITSDVRQQITLVNQKAREEW 733

Query: 742 EKKRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFG 801
           EKK+ E E+LR+LNDPEDG  VAG+K+KDEGRMKS+K NKEEDDKMRTTAANVAARAA G
Sbjct: 734 EKKQAEEEKLRKLNDPEDGSGVAGDKDKDEGRMKSLKVNKEEDDKMRTTAANVAARAAVG 793

Query: 802 GDDMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLK 861
           GDDMLSKWQLMAEQARQKREG M+SAS SQ+G DAVRK SSAAGR  KDN EGE++    
Sbjct: 794 GDDMLSKWQLMAEQARQKREGGMDSASSSQAGKDAVRKSSSAAGRHGKDNLEGERK---- 853

Query: 862 ATCLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAE 921
                  GTSR FG  Q N TQT+VARSISVKD+IAVLQREPQM RSTTIYR +NRV  E
Sbjct: 854 -------GTSRKFGRNQTNATQTKVARSISVKDVIAVLQREPQMSRSTTIYRLFNRVHPE 899

BLAST of Carg24220 vs. TrEMBL
Match: tr|A0A0A0LF46|A0A0A0LF46_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G903480 PE=4 SV=1)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 648/904 (71.68%), Postives = 710/904 (78.54%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAF-SGGNNGSSGLLLQASSQNKNT 81
           DE+MHSGAAV AFQAA NR+IEGDV  V+QT+ SD AF  G NNGSS             
Sbjct: 14  DESMHSGAAVDAFQAALNRDIEGDVQAVAQTSESDAAFPQGNNNGSSTXXXXXXXXXXXX 73

Query: 82  ESHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQHNAAPLRASKNQPHADREQVEAEQV 141
                          Q SSLME ER  SVPENQQQHN+AP + SKNQP ADREQ E EQV
Sbjct: 74  XXXXXXXXXXXXXXXQHSSLMELER--SVPENQQQHNSAPFQVSKNQPQADREQGEGEQV 133

Query: 142 SAHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQANNSLNRSK 201
           SA FSQT  LQV+E+ PIL+N SNRMQ  DNESQYLKLQKMSNQ++MV+EQANN LNRSK
Sbjct: 134 SAQFSQTAGLQVSEKAPILVNDSNRMQNRDNESQYLKLQKMSNQQSMVAEQANNPLNRSK 193

Query: 202 QVPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQMLRLAVSQV 261
           QVPFASLMPVLMPQLDKDRGMQLQ+LFN+LKRNEMNKDDF+R +R VVG+QMLRLAV QV
Sbjct: 194 QVPFASLMPVLMPQLDKDRGMQLQTLFNRLKRNEMNKDDFIRLMRGVVGDQMLRLAVCQV 253

Query: 262 QSQPPPSVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAVQSYIPSSASQGQSSSW 321
           QSQPPPSVRQ  PRMPSMGPG PNFSD RPF QLH KGMN PAVQSY+PS ASQG+SSS 
Sbjct: 254 QSQPPPSVRQLPPRMPSMGPGTPNFSDPRPFTQLHPKGMNPPAVQSYMPSPASQGRSSSG 313

Query: 322 YPAKDKNTKSLREVEQRSD-GVNQWTSSSSGTIQERKRSSVPVSGLEKQKLHLQQKSFSM 381
           YPA DKN +SLREVEQR D   NQ TSSS+ TIQ+R+RSSV V GLEKQ+LH QQKSF+M
Sbjct: 314 YPAMDKNMQSLREVEQRPDCNGNQITSSSTSTIQDRERSSVSVPGLEKQQLHFQQKSFNM 373

Query: 382 YGNSGNYHPYTGSSINASSLSSKPQTHEGHVKQIPQQAPSLGRQVTINDFKRVQGGNVPH 441
           YGNSGNYHPYTGS++NASSLS KPQ HEG VKQI QQAP+  RQVTIND KRVQ G+VPH
Sbjct: 374 YGNSGNYHPYTGSNMNASSLSLKPQPHEGQVKQISQQAPNFDRQVTINDSKRVQAGSVPH 433

Query: 442 LHNNLTAQQVSWKSSISKEQNTGPLSSMSYIKQQSSGQVSEQNKPQLSNLQGLSSVSSMQ 501
           LHNNLT+QQ SWKSS SKEQ     +  SY+KQ+ S QVSEQ+K Q SNLQGLSS+ SMQ
Sbjct: 434 LHNNLTSQQNSWKSSTSKEQ-----TITSYVKQEPSDQVSEQSKTQHSNLQGLSSIPSMQ 493

Query: 502 VEHVKTTPGIGKDALAKQTSKMGFPI-NNVMPPTSSNAANFISSDASSLHDSNAVLCSQV 561
            E V T PGI KD   KQTSKMGFP  NNVMPPTS+NAAN ISSD+SSLH+SNA     V
Sbjct: 494 AEQVNTNPGIAKDPFDKQTSKMGFPTPNNVMPPTSTNAANSISSDSSSLHESNAA----V 553

Query: 562 PSAATPGMQNRAPQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVS 621
           PSA TPGMQNRAPQ K  VGQKKPLE LGSSPPL SKKQK+SGAF+DQSIEQLNDVT VS
Sbjct: 554 PSATTPGMQNRAPQKKAAVGQKKPLEALGSSPPLSSKKQKVSGAFADQSIEQLNDVTAVS 613

Query: 622 GVNIEE-EEEQLFSSAKENSRAFEASRKVVQEEERLLFQKGQLQKKLVEIIAKCGLKGMS 681
           GVNI E                                    LQKKLVEI+AKCGLKGMS
Sbjct: 614 GVNIREXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPLQKKLVEIMAKCGLKGMS 673

Query: 682 NEVERCLSLCVEERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEW 741
           N+VE+CLSLCVEERLRG+ISNLIRLSKQRVDAEKPRHRT++TSDVRQQITL NQKAREEW
Sbjct: 674 NDVEKCLSLCVEERLRGVISNLIRLSKQRVDAEKPRHRTVITSDVRQQITLVNQKAREEW 733

Query: 742 EKKRGEGEELRRLNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFG 801
           EKK+ E E+LR+LNDP+DG  V+G+KEKDEGRMKS+K NKEEDDKMRTTAANVAARAA G
Sbjct: 734 EKKQAEEEKLRKLNDPDDGSGVSGDKEKDEGRMKSLKVNKEEDDKMRTTAANVAARAAVG 793

Query: 802 GDDMLSKWQLMAEQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLK 861
           GDDMLSKWQLMAEQARQKREG ++SAS SQ+G DAVRK SSAAGR  KDN EGE++    
Sbjct: 794 GDDMLSKWQLMAEQARQKREGGVDSASSSQAGKDAVRKSSSAAGRHGKDNLEGERK---- 853

Query: 862 ATCLYSPGTSRSFGSTQINETQTRVARSISVKDLIAVLQREPQMCRSTTIYRSYNRVRAE 921
                  GTSR FG  Q N TQT+VARSISVKD+IAVLQREPQM RSTTIYR +NRV  E
Sbjct: 854 -------GTSRKFGRNQTNATQTKVARSISVKDVIAVLQREPQMSRSTTIYRLFNRVHPE 895

BLAST of Carg24220 vs. TrEMBL
Match: tr|A0A251P1F1|A0A251P1F1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G007400 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.5e-222
Identity = 512/952 (53.78%), Postives = 629/952 (66.07%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAF--SGGNNGSSGLLLQ--ASSQN 81
           DETMHSGA V AFQAA NR+IEGDV +VSQ + SD+A    G NN SS  L Q   ++Q+
Sbjct: 15  DETMHSGADVEAFQAALNRDIEGDV-SVSQPSDSDSAVLSQGSNNTSSQSLPQFHTATQD 74

Query: 82  KNTESHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQ-----HNAAPLRASKNQPHADR 141
           +NT    Q DK    ++E  S  ME ++  S  EN QQ     H        + QP  D 
Sbjct: 75  ENTACQTQHDKKIAQQREMHSYEMELKQYGSGAENIQQKKDASHEFNQFPLPQKQPQGDL 134

Query: 142 EQVEAEQVSAHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQA 201
           +Q +AEQ   H  +T  + ++ ++PI  +  +    P++ESQYLKLQKMS+Q+AM+ EQ 
Sbjct: 135 QQGQAEQKPLHKPETAGIPISGKIPISKHEQDVTPTPESESQYLKLQKMSSQQAMIPEQP 194

Query: 202 NNSLNRSKQVPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQM 261
           +N +NRSKQVPF  L+PVL+PQLDKDR MQL +LF KLK NE++KD FVR IRSVVG+QM
Sbjct: 195 SNPMNRSKQVPFGLLLPVLLPQLDKDRAMQLTTLFGKLKNNEISKDAFVRHIRSVVGDQM 254

Query: 262 LRLAVSQVQSQPPP--------SVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAV 321
           L+LAV +VQSQP P        SV+Q  PRMPS+  G+  F+D R FA LHQ+G N P  
Sbjct: 255 LKLAVMKVQSQPVPKHQLPPQSSVQQQPPRMPSISVGSTPFTDPRSFA-LHQRGANPPTD 314

Query: 322 QSYIPSSASQGQSSSWYPAKDKNTKSLREVEQRSDG----VNQWTS-----SSSGTIQER 381
            S+IPSSA Q QS S +   + + K LRE E+ SD     V+Q  S              
Sbjct: 315 PSHIPSSAVQVQSDSSHSVIENSAKKLREAERPSDSHGMQVSQMPSXXXXXXXXXXXXXX 374

Query: 382 KRSSVPVSGLEKQKLHLQQKSFSMYGNS-GNYHPYTGSSINASSLSSKPQTHEGHVKQIP 441
                          H  Q SF+MYG++ GNYHPY+G+SIN S+L  K Q H+  ++QIP
Sbjct: 375 XXXXXXXXXXXXXXXHYPQSSFAMYGSTGGNYHPYSGTSINTSTLPLKQQPHDSQLRQIP 434

Query: 442 Q-------------------QAPSLGRQVTINDFKRVQGGNVPHLHNNLTAQQVSW-KSS 501
           Q                       L RQ ++ND  R+QGG+V H  NN   QQ S  + S
Sbjct: 435 QHQGMGSTQSGGEPQGVNITNVSKLERQNSLNDPSRLQGGSVSHFTNNSNLQQNSVPRQS 494

Query: 502 ISKEQNTGPLSSMSYIKQQSSGQVSE-QNKPQLSNLQGLSSVSSMQVEHVKTTPGIGKD- 561
            +KEQN GP+SSMSY+KQ+   Q +E Q KP LSN QGL S S+ Q+E     PGI  D 
Sbjct: 495 SNKEQNPGPVSSMSYVKQEPIDQTAEQQQKPPLSNQQGLPSASAAQLEQGSALPGISTDE 554

Query: 562 ALAKQTSKMGFPINNVMPPTSSNAA--NFISSDASSLHDSNAVLCSQVPSAATPGMQNRA 621
           ++ KQ+S+MGF  + ++  +S+     N +S    +  D+N  L  ++PS  T G+ NRA
Sbjct: 555 SIEKQSSRMGFATSGMVTSSSTGTVPPNSVSPSIMTQVDTNVSLGHRIPS-GTAGISNRA 614

Query: 622 PQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVNIEEEEEQLF 681
           P  K  +GQKKPLE  GSSPP  SKKQKLSG F DQSIEQLNDVT VSGVN+ EEEEQLF
Sbjct: 615 PPKKPSIGQKKPLEVPGSSPPPSSKKQKLSGNFLDQSIEQLNDVTAVSGVNLREEEEQLF 674

Query: 682 SSAKENSRAFEASRKVVQ-EEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVERCLSLCVE 741
           S  KE+SRA EASRK VQ EEERL+ QK  LQKKL EI+ KCGLK +SN+VERCLSLCVE
Sbjct: 675 SGPKEDSRASEASRKFVQEEEERLILQKAPLQKKLAEIMVKCGLKSISNDVERCLSLCVE 734

Query: 742 ERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKKRGEGEELRR 801
           ER+RG+I+NLIRLSKQRVDAEKPRH T+ TSDVRQQ+   NQ AREE+EKK+ E E+LRR
Sbjct: 735 ERMRGLINNLIRLSKQRVDAEKPRHHTITTSDVRQQVMNLNQNAREEFEKKQAEAEKLRR 794

Query: 802 LNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDMLSKWQLMA 861
           LN+PE    V G+K+KD+GR KS K NKEEDDKMRTTAANVAARAA GGDDMLSKWQLMA
Sbjct: 795 LNEPEVNNGVDGDKDKDDGRSKSFKPNKEEDDKMRTTAANVAARAAVGGDDMLSKWQLMA 854

Query: 862 EQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKATCLYSPGTSRS 921
           EQARQKREG ++ ASGSQ G D  RK +S AGR  KDNQE EKR     T + + GT R 
Sbjct: 855 EQARQKREGGVDVASGSQPGKDVNRKPTSTAGRIMKDNQEAEKRG--GGTPVAAAGTFRK 914

BLAST of Carg24220 vs. TrEMBL
Match: tr|A0A251P4Z6|A0A251P4Z6_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G007400 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.8e-222
Identity = 511/950 (53.79%), Postives = 628/950 (66.11%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAF--SGGNNGSSGLLLQ--ASSQN 81
           DETMHSGA V AFQAA NR+IEGDV +VSQ + SD+A    G NN SS  L Q   ++Q+
Sbjct: 15  DETMHSGADVEAFQAALNRDIEGDV-SVSQPSDSDSAVLSQGSNNTSSQSLPQFHTATQD 74

Query: 82  KNTESHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQ-----HNAAPLRASKNQPHADR 141
           +NT    Q DK    ++E  S  ME ++  S  EN QQ     H        + QP  D 
Sbjct: 75  ENTACQTQHDKKIAQQREMHSYEMELKQYGSGAENIQQKKDASHEFNQFPLPQKQPQGDL 134

Query: 142 EQVEAEQVSAHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQA 201
           +Q +AEQ   H  +T  + ++ ++PI  +  +    P++ESQYLKLQKMS+Q+AM+ EQ 
Sbjct: 135 QQGQAEQKPLHKPETAGIPISGKIPISKHEQDVTPTPESESQYLKLQKMSSQQAMIPEQP 194

Query: 202 NNSLNRSKQVPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQM 261
           +N +NRSKQVPF  L+PVL+PQLDKDR MQL +LF KLK NE++KD FVR IRSVVG+QM
Sbjct: 195 SNPMNRSKQVPFGLLLPVLLPQLDKDRAMQLTTLFGKLKNNEISKDAFVRHIRSVVGDQM 254

Query: 262 LRLAVSQVQSQPPP--------SVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAV 321
           L+LAV +VQSQP P        SV+Q  PRMPS+  G+  F+D R FA LHQ+G N P  
Sbjct: 255 LKLAVMKVQSQPVPKHQLPPQSSVQQQPPRMPSISVGSTPFTDPRSFA-LHQRGANPPTD 314

Query: 322 QSYIPSSASQGQSSSWYPAKDKNTKSLREVEQRSDG----VNQWTS-----SSSGTIQER 381
            S+IPSSA Q QS S +   + + K LRE E+ SD     V+Q  S              
Sbjct: 315 PSHIPSSAVQVQSDSSHSVIENSAKKLREAERPSDSHGMQVSQMPSXXXXXXXXXXXXXX 374

Query: 382 KRSSVPVSGLEKQKLHLQQKSFSMYGNS-GNYHPYTGSSINASSLSSKPQTHEGHVKQIP 441
                          H  Q SF+MYG++ GNYHPY+G+SIN S+L  K Q H+  ++QIP
Sbjct: 375 XXXXXXXXXXXXXXXHYPQSSFAMYGSTGGNYHPYSGTSINTSTLPLKQQPHDSQLRQIP 434

Query: 442 Q-------------------QAPSLGRQVTINDFKRVQGGNVPHLHNNLTAQQVSW-KSS 501
           Q                       L RQ ++ND  R+QGG+V H  NN   QQ S  + S
Sbjct: 435 QHQGMGSTQSGGEPQGVNITNVSKLERQNSLNDPSRLQGGSVSHFTNNSNLQQNSVPRQS 494

Query: 502 ISKEQNTGPLSSMSYIKQQSSGQVSE-QNKPQLSNLQGLSSVSSMQVEHVKTTPGIGKD- 561
            +KEQN GP+SSMSY+KQ+   Q +E Q KP LSN QGL S S+ Q+E     PGI  D 
Sbjct: 495 SNKEQNPGPVSSMSYVKQEPIDQTAEQQQKPPLSNQQGLPSASAAQLEQGSALPGISTDE 554

Query: 562 ALAKQTSKMGFPINNVMPPTSSNAA--NFISSDASSLHDSNAVLCSQVPSAATPGMQNRA 621
           ++ KQ+S+MGF  + ++  +S+     N +S    +  D+N  L  ++PS  T G+ NRA
Sbjct: 555 SIEKQSSRMGFATSGMVTSSSTGTVPPNSVSPSIMTQVDTNVSLGHRIPS-GTAGISNRA 614

Query: 622 PQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVNIEEEEEQLF 681
           P  K  +GQKKPLE  GSSPP  SKKQKLSG F DQSIEQLNDVT VSGVN+ EEEEQLF
Sbjct: 615 PPKKPSIGQKKPLEVPGSSPPPSSKKQKLSGNFLDQSIEQLNDVTAVSGVNLREEEEQLF 674

Query: 682 SSAKENSRAFEASRKVVQ-EEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVERCLSLCVE 741
           S  KE+SRA EASRK VQ EEERL+ QK  LQKKL EI+ KCGLK +SN+VERCLSLCVE
Sbjct: 675 SGPKEDSRASEASRKFVQEEEERLILQKAPLQKKLAEIMVKCGLKSISNDVERCLSLCVE 734

Query: 742 ERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKKRGEGEELRR 801
           ER+RG+I+NLIRLSKQRVDAEKPRH T+ TSDVRQQ+   NQ AREE+EKK+ E E+LRR
Sbjct: 735 ERMRGLINNLIRLSKQRVDAEKPRHHTITTSDVRQQVMNLNQNAREEFEKKQAEAEKLRR 794

Query: 802 LNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDMLSKWQLMA 861
           LN+PE    V G+K+KD+GR KS K NKEEDDKMRTTAANVAARAA GGDDMLSKWQLMA
Sbjct: 795 LNEPEVNNGVDGDKDKDDGRSKSFKPNKEEDDKMRTTAANVAARAAVGGDDMLSKWQLMA 854

Query: 862 EQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKATCLYSPGTSRS 920
           EQARQKREG ++ ASGSQ G D  RK +S AGR  KDNQE EKR     T + + GT R 
Sbjct: 855 EQARQKREGGVDVASGSQPGKDVNRKPTSTAGRIMKDNQEAEKRG--GGTPVAAAGTFRK 914

BLAST of Carg24220 vs. TrEMBL
Match: tr|A0A251P1D7|A0A251P1D7_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G007400 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.8e-222
Identity = 511/950 (53.79%), Postives = 628/950 (66.11%), Query Frame = 0

Query: 22  DETMHSGAAVAAFQAASNREIEGDVPTVSQTNASDTAF--SGGNNGSSGLLLQ--ASSQN 81
           DETMHSGA V AFQAA NR+IEGDV +VSQ + SD+A    G NN SS  L Q   ++Q+
Sbjct: 15  DETMHSGADVEAFQAALNRDIEGDV-SVSQPSDSDSAVLSQGSNNTSSQSLPQFHTATQD 74

Query: 82  KNTESHVQLDKNFRLKQEQRSSLMEPERCTSVPENQQQ-----HNAAPLRASKNQPHADR 141
           +NT    Q DK    ++E  S  ME ++  S  EN QQ     H        + QP  D 
Sbjct: 75  ENTACQTQHDKKIAQQREMHSYEMELKQYGSGAENIQQKKDASHEFNQFPLPQKQPQGDL 134

Query: 142 EQVEAEQVSAHFSQTEELQVAEQVPILMNYSNRMQYPDNESQYLKLQKMSNQEAMVSEQA 201
           +Q +AEQ   H  +T  + ++ ++PI  +  +    P++ESQYLKLQKMS+Q+AM+ EQ 
Sbjct: 135 QQGQAEQKPLHKPETAGIPISGKIPISKHEQDVTPTPESESQYLKLQKMSSQQAMIPEQP 194

Query: 202 NNSLNRSKQVPFASLMPVLMPQLDKDRGMQLQSLFNKLKRNEMNKDDFVRCIRSVVGNQM 261
           +N +NRSKQVPF  L+PVL+PQLDKDR MQL +LF KLK NE++KD FVR IRSVVG+QM
Sbjct: 195 SNPMNRSKQVPFGLLLPVLLPQLDKDRAMQLTTLFGKLKNNEISKDAFVRHIRSVVGDQM 254

Query: 262 LRLAVSQVQSQPPP--------SVRQPSPRMPSMGPGAPNFSDSRPFAQLHQKGMNSPAV 321
           L+LAV +VQSQP P        SV+Q  PRMPS+  G+  F+D R FA LHQ+G N P  
Sbjct: 255 LKLAVMKVQSQPVPKHQLPPQSSVQQQPPRMPSISVGSTPFTDPRSFA-LHQRGANPPTD 314

Query: 322 QSYIPSSASQGQSSSWYPAKDKNTKSLREVEQRSDG----VNQWTS-----SSSGTIQER 381
            S+IPSSA Q QS S +   + + K LRE E+ SD     V+Q  S              
Sbjct: 315 PSHIPSSAVQVQSDSSHSVIENSAKKLREAERPSDSHGMQVSQMPSXXXXXXXXXXXXXX 374

Query: 382 KRSSVPVSGLEKQKLHLQQKSFSMYGNS-GNYHPYTGSSINASSLSSKPQTHEGHVKQIP 441
                          H  Q SF+MYG++ GNYHPY+G+SIN S+L  K Q H+  ++QIP
Sbjct: 375 XXXXXXXXXXXXXXXHYPQSSFAMYGSTGGNYHPYSGTSINTSTLPLKQQPHDSQLRQIP 434

Query: 442 Q-------------------QAPSLGRQVTINDFKRVQGGNVPHLHNNLTAQQVSW-KSS 501
           Q                       L RQ ++ND  R+QGG+V H  NN   QQ S  + S
Sbjct: 435 QHQGMGSTQSGGEPQGVNITNVSKLERQNSLNDPSRLQGGSVSHFTNNSNLQQNSVPRQS 494

Query: 502 ISKEQNTGPLSSMSYIKQQSSGQVSE-QNKPQLSNLQGLSSVSSMQVEHVKTTPGIGKD- 561
            +KEQN GP+SSMSY+KQ+   Q +E Q KP LSN QGL S S+ Q+E     PGI  D 
Sbjct: 495 SNKEQNPGPVSSMSYVKQEPIDQTAEQQQKPPLSNQQGLPSASAAQLEQGSALPGISTDE 554

Query: 562 ALAKQTSKMGFPINNVMPPTSSNAA--NFISSDASSLHDSNAVLCSQVPSAATPGMQNRA 621
           ++ KQ+S+MGF  + ++  +S+     N +S    +  D+N  L  ++PS  T G+ NRA
Sbjct: 555 SIEKQSSRMGFATSGMVTSSSTGTVPPNSVSPSIMTQVDTNVSLGHRIPS-GTAGISNRA 614

Query: 622 PQIKVVVGQKKPLETLGSSPPLPSKKQKLSGAFSDQSIEQLNDVTVVSGVNIEEEEEQLF 681
           P  K  +GQKKPLE  GSSPP  SKKQKLSG F DQSIEQLNDVT VSGVN+ EEEEQLF
Sbjct: 615 PPKKPSIGQKKPLEVPGSSPPPSSKKQKLSGNFLDQSIEQLNDVTAVSGVNLREEEEQLF 674

Query: 682 SSAKENSRAFEASRKVVQ-EEERLLFQKGQLQKKLVEIIAKCGLKGMSNEVERCLSLCVE 741
           S  KE+SRA EASRK VQ EEERL+ QK  LQKKL EI+ KCGLK +SN+VERCLSLCVE
Sbjct: 675 SGPKEDSRASEASRKFVQEEEERLILQKAPLQKKLAEIMVKCGLKSISNDVERCLSLCVE 734

Query: 742 ERLRGIISNLIRLSKQRVDAEKPRHRTLVTSDVRQQITLANQKAREEWEKKRGEGEELRR 801
           ER+RG+I+NLIRLSKQRVDAEKPRH T+ TSDVRQQ+   NQ AREE+EKK+ E E+LRR
Sbjct: 735 ERMRGLINNLIRLSKQRVDAEKPRHHTITTSDVRQQVMNLNQNAREEFEKKQAEAEKLRR 794

Query: 802 LNDPEDGQRVAGEKEKDEGRMKSVKANKEEDDKMRTTAANVAARAAFGGDDMLSKWQLMA 861
           LN+PE    V G+K+KD+GR KS K NKEEDDKMRTTAANVAARAA GGDDMLSKWQLMA
Sbjct: 795 LNEPEVNNGVDGDKDKDDGRSKSFKPNKEEDDKMRTTAANVAARAAVGGDDMLSKWQLMA 854

Query: 862 EQARQKREGRMNSASGSQSGNDAVRKFSSAAGRRRKDNQEGEKRSHLKATCLYSPGTSRS 920
           EQARQKREG ++ ASGSQ G D  RK +S AGR  KDNQE EKR     T + + GT R 
Sbjct: 855 EQARQKREGGVDVASGSQPGKDVNRKPTSTAGRIMKDNQEAEKRG--GGTPVAAAGTFRK 914