Carg23928 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23928
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Mediator of RNA polymerase II transcription subunit 22-like protein
Location	Cucurbita_argyrosperma_scaffold_173 : 187125 .. 190343 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGCTTCTTCTTCCCCGACATTTCTACTTCTCTCTCTTTTCAATTACCTTGCCGCCATTGCAGAGCCGATTCAATTGCCATTTCCATTTTTATTATTATTATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACCGCAGCGGCTGCTGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTTCAGAGAGTCGAAACCGATATAGCCAACATTGTCGACAATTTCACCCATCTTGTCAACGTCTCAAGGGTAACGCTCCTCTTTCCTTTTCTTTTTTGCTTTTTCTTCATTGTTTTCTCAACTCAATTCTTCTTCTACTACTTGGAATGGGAAATTTGTCATGTAATTCTCTTTGGTTGGTTGTTTTCCTGTTCTTGATTGAACGATCCCTTGTTTGGAATCCATAATTCGAACTTAGCGTTCTCGTTTCCTTAGGTGAACGATCCACCTGTTAGGAATTCACAGGAATCTTTCATGATGGAAATGCGTGCGTCCAGAATGGTAAGATAATGATTTTTCCTTTTTTTGTTTACCTACTGTTTTTGCCAAGCTTATTTTAAGTTAATTTCTGGATAGATAGCTCTCTGTTTCCTTCCACAATTGAATGATAACATCTTATATGTTATTTGAAGCCTTGATTCCTAGGGATTTACTGATTTTTCAATAAAATCAGCTGCTTCTGCATCTCTTCCAGTATGTTTTTCTTCTCCTTCACCAGCAATTCCAAGCAAATTCTCTTCTTTAATTAACGTCTATGGTCTTCAAATTCGTAACCCTCCGGCGCTACCAATTGGTTCTTCAGATAAATTTACTTGAGTTACATTTTTAGCTTGAGCAGTGAAAGTTATCTCGTGGAATACTAGAGACCTTGGGGATCGTTCTAAAAGAACAACATTGAAAAATTCATTCAACGTCACTGTCTTGATATGGTGTTAGTTCATGAGACAAAGATTGCTTTTTTTTTTTTTTTTTTTTTTTTTTGACTGAAAGGTTATCAGATCTTTATGGTTCTCCAAAGAAATCGGTTGGATTAATGTTGGATCTATTGAGACATCAGGTGGTTTACTGATTTTGTGGGATTTTGTGGGATGCTAGTAAGATAAATGTGAGATCCCACATGGGTTGGGGAGGACAACGAAGCATTCTTTATAAGGGTGTGGAAACCTTTCCCCAGCAGACGCTTTTTAAAATCCTTGAGGGGAAGCCCGAAAGGGAAAACCCAAAGAGGAGAATATCTGCTCGTGGTGAGCTTGGACCATTACAAATGGTATCAGAGCCAAGCATCAGACGATATGCCAGCCAAGAGCTGAGCCTCGAAGGGGGGTTGATTGGGGGGTCCCACATCGATTGGAGAAGGGAACGAGTGCCAACGAGAACGCTGGGCCCTGAAAGGGGTGGATTGTGAGATCCCAAATTGATGGAAGAAGAGTATGAGTACCAGCGAGGACGCTGGCCCTGAAGGGGGTGGATTGTGAAATCCCACATCGGTTGGGGAGGAGAACGAAGCATTCTTTATAATGGTGTGGAAACTTCTCCCTAGCAGATGTGTTATATAAAAAACTTGAGGGAAAGCCCGGAACGGAAAGCCCAAAGAGGACAATATCTGCTAGTGGTGGGCTTGGGCCGTTACAACAAAAATCTTGGAGTTTCTTAAAGGAGGCTGCACTCTCTCAATTGAAATACTTTTCCAGAACAAGTGAATTTGTTGGGTAACACACATTTATGAACCAATTGACTATAGAGAACGAAAATGTCTTCGGAGTGAATGCGGTCTTTATTCTCTCATACTGTGAAGGAGCCTTGGTACATTTGTGGCAATTTTTATATAACTCGTTGGGTTCATGAAAGATCACCATTAGGAAGAAAAGGCAAAAGTTGGGAGATAATTCATAGCATTCTTTGATCAATAGCTTTTTTTGTTTCGTAGGAACAAGAATAGTTATTTGATGATTCAACAGTATAATGACAAGCTCAGATTTTTTTATTGAGCTCTATCCTTTACTCTCGGAAGCTGGACGTTTTGTTTGGGTGTTATCCCACTTTAGATTTTATATCAGTTGGTTAGAAATATCAGTTTGTGAGGTCATCATCAGGGAAGCTCTTTCCATAGATTTTCATGTCGATGCCTTTGTGGAATTCAGTTGGTTAAATCTTTTTGGTGAAAGAATTTTGGCCAGCTTTTATTAGTATTGGCTCTTGTTGTCATCTGTTGTTGCTTTTCGAGGTTTCTCAGCAGTCGAATTTTGGTTTGATGGGTCTTGTTTCGTTTCTTGACAATTCTCATTTGATTGTAATTGTTTTAAAATCTTGTTCAATTTCTTATTTCATTCCCACATAAGATTTTAGGCAATAATAAATTTGGGATCTGTTCTTTTTTGTTCTTATCAGCTCGATATAGATGATAGCATTTCATTATAATCATAATGGCTACTAATTTAGAGCTTTTTACTTGTTTCTAAACAGGTTCAGGCAGCTGATTCTCTGCTTAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGATCACGTGGAGCAAAGAATCGTGGAATTCAATCAGCAGGCGGAGCAGACCGACCGTATCCTATCTAAAATTGGTGAAGAAGCTGCTGTAGGGCTTAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACATGATTTGTAAGTAGTGAATGTAAATAAGGATGTGATATTGTTGGCTTCTGAAACTCAATTTCTCGCTAACATGGATAGTAAAACATAACCGAACTCTTCTAGCCTATGTTCTTAGCATTTTTCTTTGGCTTTTATTGTTTTGGCATGCTAGATATGCAGGAACTAGCAGAAGGGTTTGGTGTCTTTTCTTACTAAGCTTTTTAGAAGAGTAATTAGGATGGGGAAGAAGGGCTTGACAAGGATGTAAAACATTATTTGGGAAATATGAACAATCAGAATATTATTGTTCAAACTAATGTTATTGCTGAGGACTACGGTGGCAAAAATGGCCGAGCTTGTAGAAGGTCAAAATAACAAATAAAAACTCATAGATTCATTGATGAATGTCCCAAATATTACTTACACCTCGGGTACGTGAGGAAACATATTATTATTGTAACCCTCCAAGTCCACTACTACTAGATATTGTCCGATTTGGTCTGTTACACGTCACTGTCAGCTTCACAATTTTAATACATGTT

mRNA sequence

GTGCTTCTTCTTCCCCGACATTTCTACTTCTCTCTCTTTTCAATTACCTTGCCGCCATTGCAGAGCCGATTCAATTGCCATTTCCATTTTTATTATTATTATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACCGCAGCGGCTGCTGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTTCAGAGAGTCGAAACCGATATAGCCAACATTGTCGACAATTTCACCCATCTTGTCAACGTCTCAAGGGTGAACGATCCACCTGTTAGGAATTCACAGGAATCTTTCATGATGGAAATGCGTGCGTCCAGAATGGTTCAGGCAGCTGATTCTCTGCTTAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGATCACGTGGAGCAAAGAATCGTGGAATTCAATCAGCAGGCGGAGCAGACCGACCGTATCCTATCTAAAATTGGTGAAGAAGCTGCTGTAGGGCTTAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACATGATTTGTAAGTAGTGAATGTAAATAAGGATGTGATATTGTTGGCTTCTGAAACTCAATTTCTCGCTAACATGGATAGTAAAACATAACCGAACTCTTCTAGCCTATGTTCTTAGCATTTTTCTTTGGCTTTTATTGTTTTGGCATGCTAGATATGCAGGAACTAGCAGAAGGGTTTGGTGTCTTTTCTTACTAAGCTTTTTAGAAGAGTAATTAGGATGGGGAAGAAGGGCTTGACAAGGATGTAAAACATTATTTGGGAAATATGAACAATCAGAATATTATTGTTCAAACTAATGTTATTGCTGAGGACTACGGTGGCAAAAATGGCCGAGCTTGTAGAAGGTCAAAATAACAAATAAAAACTCATAGATTCATTGATGAATGTCCCAAATATTACTTACACCTCGGGTACGTGAGGAAACATATTATTATTGTAACCCTCCAAGTCCACTACTACTAGATATTGTCCGATTTGGTCTGTTACACGTCACTGTCAGCTTCACAATTTTAATACATGTT

Coding sequence (CDS)

ATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACCGCAGCGGCTGCTGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTTCAGAGAGTCGAAACCGATATAGCCAACATTGTCGACAATTTCACCCATCTTGTCAACGTCTCAAGGGTGAACGATCCACCTGTTAGGAATTCACAGGAATCTTTCATGATGGAAATGCGTGCGTCCAGAATGGTTCAGGCAGCTGATTCTCTGCTTAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGATCACGTGGAGCAAAGAATCGTGGAATTCAATCAGCAGGCGGAGCAGACCGACCGTATCCTATCTAAAATTGGTGAAGAAGCTGCTGTAGGGCTTAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACATGA

Protein sequence

MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQRTSQNSLT

Homology

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BLAST of Carg23928 vs. NCBI nr
Match: XP_023545554.1 (mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo] >XP_023545555.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo] >XP_023545556.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 236.1 bits (601), Expect = 7.7e-59
Identity = 149/150 (99.33%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120
           SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDR+LS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 151
           KIGEEAAVGLKELESHYYSSTQRTSQNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150

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BLAST of Carg23928 vs. NCBI nr
Match: XP_022929626.1 (mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata] >XP_022929627.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata] >XP_022929628.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata])

HSP 1 Score: 233.8 bits (595), Expect = 3.8e-58
Identity = 148/150 (98.67%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120
           SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRI+S
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRIIS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 151
           KIGEEAAVGLKELESHYYSSTQRTSQN LT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNRLT 150

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BLAST of Carg23928 vs. NCBI nr
Match: XP_022996928.1 (mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbita maxima] >XP_022996929.1 mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbita maxima] >XP_022996930.1 mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 230.7 bits (587), Expect = 3.2e-57
Identity = 147/150 (98.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120
           SFMMEMRASRMVQAADSLLKLVSELKQ AIFSGFASLNDHVEQRIVEFN QAEQTDRILS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQAAIFSGFASLNDHVEQRIVEFNMQAEQTDRILS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 151
           KIGEEAAVGLKELESHYYSSTQRT QNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTCQNSLT 150

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BLAST of Carg23928 vs. NCBI nr
Match: XP_004145443.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Cucumis sativus] >KGN66872.1 hypothetical protein Csa_1G701970 [Cucumis sativus])

HSP 1 Score: 226.9 bits (577), Expect = 4.7e-56
Identity = 143/150 (95.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 151
           +IGEEAA  LKELESHYYSSTQRT QNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

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BLAST of Carg23928 vs. NCBI nr
Match: XP_022155216.1 (mediator of RNA polymerase II transcription subunit 22b-like [Momordica charantia])

HSP 1 Score: 226.9 bits (577), Expect = 4.7e-56
Identity = 141/150 (94.00%), Postives = 145/150 (96.67%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG+GXXXXXXXXXXXXXXXXXXX  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGVGXXXXXXXXXXXXXXXXXXXLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 151
           +IGEEAA  LKELESHYYSSTQRTSQN LT
Sbjct: 121 RIGEEAATSLKELESHYYSSTQRTSQNILT 150

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BLAST of Carg23928 vs. TAIR10
Match: AT1G07950.1 (Surfeit locus protein 5 subunit 22 of Mediator complex)

HSP 1 Score: 183.7 bits (465), Expect = 8.2e-47
Identity = 92/116 (79.31%), Postives = 109/116 (93.97%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           QRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++MMEMRASRMVQAADSLLKLVSELKQ
Sbjct: 34  QRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYMMEMRASRMVQAADSLLKLVSELKQ 93

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQR 144
           TAIFSGFASLNDHVEQRI EF+Q+AE+T+R+L++I ++A+  LKELESHYYSS QR
Sbjct: 94  TAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIADDASANLKELESHYYSSAQR 149

[Close]

BLAST of Carg23928 vs. TAIR10
Match: AT1G16430.1 (Surfeit locus protein 5 subunit 22 of Mediator complex)

HSP 1 Score: 178.3 bits (451), Expect = 3.5e-45
Identity = 87/118 (73.73%), Postives = 110/118 (93.22%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           QRV+TDI ++VDNF  +VNV+RV+DPP++NSQE++MMEMRASR+VQAADSLLKLVSELKQ
Sbjct: 31  QRVDTDITSVVDNFNQIVNVARVSDPPMKNSQEAYMMEMRASRLVQAADSLLKLVSELKQ 90

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQRTS 146
           TAIFSGFASLNDHVEQRI EF+Q+AE+T+R+L++I ++A+  LKELESHYYSS QR++
Sbjct: 91  TAIFSGFASLNDHVEQRIAEFDQEAEKTNRLLARIADDASASLKELESHYYSSAQRST 148

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BLAST of Carg23928 vs. Swiss-Prot
Match: sp|Q8LCH5|MD22B_ARATH (Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana OX=3702 GN=MED22B PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.5e-45
Identity = 92/116 (79.31%), Postives = 109/116 (93.97%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           QRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++MMEMRASRMVQAADSLLKLVSELKQ
Sbjct: 34  QRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYMMEMRASRMVQAADSLLKLVSELKQ 93

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQR 144
           TAIFSGFASLNDHVEQRI EF+Q+AE+T+R+L++I ++A+  LKELESHYYSS QR
Sbjct: 94  TAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIADDASANLKELESHYYSSAQR 149

[Close]

BLAST of Carg23928 vs. Swiss-Prot
Match: sp|Q9SA42|MD22A_ARATH (Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana OX=3702 GN=MED22A PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 6.2e-44
Identity = 87/118 (73.73%), Postives = 110/118 (93.22%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           QRV+TDI ++VDNF  +VNV+RV+DPP++NSQE++MMEMRASR+VQAADSLLKLVSELKQ
Sbjct: 31  QRVDTDITSVVDNFNQIVNVARVSDPPMKNSQEAYMMEMRASRLVQAADSLLKLVSELKQ 90

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQRTS 146
           TAIFSGFASLNDHVEQRI EF+Q+AE+T+R+L++I ++A+  LKELESHYYSS QR++
Sbjct: 91  TAIFSGFASLNDHVEQRIAEFDQEAEKTNRLLARIADDASASLKELESHYYSSAQRST 148

[Close]

BLAST of Carg23928 vs. Swiss-Prot
Match: sp|Q54XB0|MED22_DICDI (Putative mediator of RNA polymerase II transcription subunit 22 OS=Dictyostelium discoideum OX=44689 GN=med22 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 39/116 (33.62%), Postives = 79/116 (68.10%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           +++++ I  +++N+ +++ +S+VND P++N+ E + ME R S M+ A + LLK++ ELKQ
Sbjct: 27  KQIDSKIMQLLENYHNIIKISKVND-PLKNASEIYEMETRTSNMLNAGEGLLKIIEELKQ 86

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQR 144
             I + F+++ + V  + + F+++ E+T++ +  I EE +  LKELE  YY+S+ +
Sbjct: 87  NLILNDFSTMAEEVRIQNLVFHKENERTNKSIKLISEELSRSLKELEDEYYNSSYK 141

[Close]

BLAST of Carg23928 vs. Swiss-Prot
Match: sp|Q62276|MED22_MOUSE (Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 GN=Med22 PE=1 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 3.6e-15
Identity = 43/119 (36.13%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMMEMRASRMVQAADSLLKLV 87
           +R++ DI +I+DNFT ++  +++ D        +  Q+++ M +RA+ +V+A +SL+KLV
Sbjct: 20  KRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLV 79

Query: 88  SELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSST 142
           S+LKQ  I + F S+N+ ++QR  +     E+ DR L  + +E ++ L ELE  YYSS+
Sbjct: 80  SDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSIDLYELEEEYYSSS 138

[Close]

BLAST of Carg23928 vs. Swiss-Prot
Match: sp|A0JPN6|MED22_RAT (Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=10116 GN=Med22 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.6e-15
Identity = 43/119 (36.13%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMMEMRASRMVQAADSLLKLV 87
           +R++ DI +I+DNFT ++  +++ D        +  Q+++ M +RA+ +V+A +SL+KLV
Sbjct: 20  KRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLV 79

Query: 88  SELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSST 142
           S+LKQ  I + F S+N+ ++QR  +     E+ DR L  + +E ++ L ELE  YYSS+
Sbjct: 80  SDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSIDLYELEEEYYSSS 138

[Close]

BLAST of Carg23928 vs. TrEMBL
Match: tr|A0A0A0M174|A0A0A0M174_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.1e-56
Identity = 143/150 (95.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 151
           +IGEEAA  LKELESHYYSSTQRT QNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of Carg23928 vs. TrEMBL
Match: tr|A0A1S3C993|A0A1S3C993_CUCME (mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498265 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.0e-55
Identity = 141/150 (94.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGXXXXXXXXXXXXXXXXXXXXXQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QT+R+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 151
           +IGEEAA  LKELESHYYSSTQRT QN+LT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNNLT 150

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BLAST of Carg23928 vs. TrEMBL
Match: tr|A0A1S3CAG5|A0A1S3CAG5_CUCME (mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498265 PE=4 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.8e-54
Identity = 114/123 (92.68%), Postives = 120/123 (97.56%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRA+RMVQAADSLLKLVSELKQ
Sbjct: 75  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQ 134

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQRTSQN 147
           TAIFSGFASLNDHVEQRIVEFNQQA+QT+R+LS+IGEEAA  LKELESHYYSSTQRT QN
Sbjct: 135 TAIFSGFASLNDHVEQRIVEFNQQAQQTERLLSRIGEEAAANLKELESHYYSSTQRTDQN 194

Query: 148 SLT 151
           +LT
Sbjct: 195 NLT 197

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BLAST of Carg23928 vs. TrEMBL
Match: tr|M5W3R2|M5W3R2_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G260300 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.8e-49
Identity = 106/120 (88.33%), Postives = 115/120 (95.83%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           QRVETDIANIVDNF+ LVNVSRVNDPPVRNSQE+FMMEMRA+RMVQAADSLLKLVSELKQ
Sbjct: 37  QRVETDIANIVDNFSSLVNVSRVNDPPVRNSQEAFMMEMRAARMVQAADSLLKLVSELKQ 96

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQRTSQN 147
           TAIFSGFASLNDHVEQRIVEFNQQAE+TD++L++IGEEAA  LKELESHYYSS  RTS+N
Sbjct: 97  TAIFSGFASLNDHVEQRIVEFNQQAEKTDQMLARIGEEAAASLKELESHYYSSALRTSEN 156

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BLAST of Carg23928 vs. TrEMBL
Match: tr|A0A2I4GB41|A0A2I4GB41_9ROSI (mediator of RNA polymerase II transcription subunit 22a-like OS=Juglans regia OX=51240 GN=LOC109006332 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.8e-49
Identity = 104/119 (87.39%), Postives = 113/119 (94.96%), Query Frame = 0


Query: 28  QRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQ 87
           QRVETDI NIVDNF+HLVNVSRVNDPPVRNSQE+FMMEMRA+RMVQAADSLLKLVSELKQ
Sbjct: 38  QRVETDIGNIVDNFSHLVNVSRVNDPPVRNSQEAFMMEMRAARMVQAADSLLKLVSELKQ 97

Query: 88  TAIFSGFASLNDHVEQRIVEFNQQAEQTDRILSKIGEEAAVGLKELESHYYSSTQRTSQ 147
           TAIFSGFASLNDHVEQR VEFNQQAE+TDR+L++IGEEAA  LKELE+HYYSS +R SQ
Sbjct: 98  TAIFSGFASLNDHVEQRTVEFNQQAEKTDRMLARIGEEAAASLKELEAHYYSSAERASQ 156

The following BLAST results are available for this feature:

BLAST of Carg23928 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545554.1	7.7e-59	99.33	mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo sub...	[more]
XP_022929626.1	3.8e-58	98.67	mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata...	[more]
XP_022996928.1	3.2e-57	98.00	mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbi...	[more]
XP_004145443.1	4.7e-56	95.33	PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like [Cucumis...	[more]
XP_022155216.1	4.7e-56	94.00	mediator of RNA polymerase II transcription subunit 22b-like [Momordica charanti...	[more]

BLAST of Carg23928 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G07950.1	8.2e-47	79.31	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]
AT1G16430.1	3.5e-45	73.73	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]

BLAST of Carg23928 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8LCH5\|MD22B_ARATH	1.5e-45	79.31	Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana ...	[more]
sp\|Q9SA42\|MD22A_ARATH	6.2e-44	73.73	Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana ...	[more]
sp\|Q54XB0\|MED22_DICDI	2.7e-15	33.62	Putative mediator of RNA polymerase II transcription subunit 22 OS=Dictyostelium...	[more]
sp\|Q62276\|MED22_MOUSE	3.6e-15	36.13	Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 ...	[more]
sp\|A0JPN6\|MED22_RAT	3.6e-15	36.13	Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=1...	[more]

BLAST of Carg23928 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0M174\|A0A0A0M174_CUCSA	3.1e-56	95.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1	[more]
tr\|A0A1S3C993\|A0A1S3C993_CUCME	2.0e-55	94.00	mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucum...	[more]
tr\|A0A1S3CAG5\|A0A1S3CAG5_CUCME	3.8e-54	92.68	mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucum...	[more]
tr\|M5W3R2\|M5W3R2_PRUPE	2.8e-49	88.33	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G260300 PE=4 SV=1	[more]
tr\|A0A2I4GB41\|A0A2I4GB41_9ROSI	4.8e-49	87.39	mediator of RNA polymerase II transcription subunit 22a-like OS=Juglans regia OX...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003712	transcription cofactor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006357	regulation of transcription from RNA polymerase II promoter

Vocabulary: **Cellular Component**
Term	Definition
GO:0016592	mediator complex

Vocabulary: **INTERPRO**
Term	Definition
IPR038427	MED22_sf
IPR009332	Med22

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription from RNA polymerase II promoter
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003712	transcription cofactor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23928-RA	Carg23928-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PFAM	PF06179	Med22	coord: 28..126 e-value: 9.5E-28 score: 96.4
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PANTHER	PTHR12434	FAMILY NOT NAMED	coord: 9..146
IPR038427	MED22 superfamily	GENE3D	G3DSA:1.20.58.1600		coord: 13..136 e-value: 1.4E-28 score: 101.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg23928	Cla022796	Watermelon (97103) v1	carwmB0616
Carg23928	ClCG11G011870	Watermelon (Charleston Gray)	carwcgB0601
Carg23928	CmoCh05G007430	Cucurbita moschata (Rifu)	carcmoB0849
Carg23928	Lsi11G007800	Bottle gourd (USVL1VR-Ls)	carlsiB563
Carg23928	Cp4.1LG11g06210	Cucurbita pepo (Zucchini)	carcpeB0879
Carg23928	Cla97C11G217850	Watermelon (97103) v2	carwmbB0644
Carg23928	Bhi06G001093	Wax gourd	carwgoB0735

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg23928	Silver-seed gourd	carcarB547
Carg23928	Cucumber (Gy14) v2	carcgybB0616
Carg23928	Cucumber (Gy14) v1	carcgyB0692
Carg23928	Cucurbita maxima (Rimu)	carcmaB0870
Carg23928	Cucurbita maxima (Rimu)	carcmaB0872
Carg23928	Cucurbita moschata (Rifu)	carcmoB0847
Carg23928	Cucurbita pepo (Zucchini)	carcpeB0880
Carg23928	Wild cucumber (PI 183967)	carcpiB0650
Carg23928	Cucumber (Chinese Long) v2	carcuB0643
Carg23928	Cucumber (Chinese Long) v3	carcucB0651
Carg23928	Melon (DHL92) v3.5.1	carmeB0619
Carg23928	Melon (DHL92) v3.6.1	carmedB0626