Carg23927 (gene) Silver-seed gourd

NameCarg23927
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCadmium/zinc-transporting ATPase
LocationCucurbita_argyrosperma_scaffold_173 : 182658 .. 186473 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGGTGGGTGAGGTGGCAATGGAGAAGAACAAGAAAGTAAGGAGAGAGAACAAATTTGAAAGAAGCCATTTTGATGTGTTGGGAATTTGCTGTTCTTCAGAAGTTCCTCTCATTGAAAACATCCTCAAACCCCTCGAAGGAATCAAACAAATCTCTGTTATTGTCCCGACAAGAACTCTCATTGTCGTCCATGATTCCCTCCTCGTTTCTCAGCTTCAAATCGGTACTCTTCTCTCCTTTCGCTTCTTAATCGATAATATATGTGTTAATCAGTTGAACTATGTTCAAATTGGACGAGTTATTAATATTTATTAAATAATTCATTTAAGTTTGACTTTTGAATTTATTTGATAGAAATCTATAACATAGAAAATGTCTAATAAGTCCGATCGAAACCTATCATATCACTGATGGACATCGTTTGTTATCAAACTTGATAGTGAAAGCACTAAACGAAGCAAGATTGGAGGCAAATGTACAATTAAAAGGAAAGGGAATTAGCAAGAAGAAATGGCCAAGTCCTTTTGCAATAGCCAGTGGGTTGCTTCTAACAGCATCCTTTTTGAAGTATGTCTACCACCCATTGCGGTGGCTGGCTGTGGCTGCGGTGGCCGCCGGCATCTTCCCCATTCTCCTAAAAGCCATCTCCGCGGTTCGCCATCTGAGGATCGATGTCAACATTCTTGCCATTATTGCGGGTAAAGTATGGTTCTTGGGCGTTGGTAAAAATTAAGATCGAAAAGTGGAGTTTATGTTTGATATGTGTATGGCAGTGGTGGGGACAATGAGTATGAATGACTACATGGAAGGTGGGAGCATAGTGTTTTTGTTTTCCATTGCTGAATGGCTTGAGTCAAGAGCAAGCCACAAGGTTAGCAAAACAATGATTATGAGCAAAACAATGATTATGAGGAAAACAATGATTATGAGCAAAACAATGATACTGAGCAAAACAATGATACTGAATTCAAAATGGGTTTAGTTTAAAGTGAAGTGTGTTAATGAAAAAGTTGAATTGAATTAGGCGAATGCTGCAATGTGGTCGCTGATGAGCCTGGCCCCTCAAAAGGCCACCATAGCTGAAACTGGAGAGGTTGTGGAGGTTAAAGAGGTGAAATTGAAGAGTGTGTTGGCAGTGAAGGGTGGTGAAGTTATTCCCATTGATGGCATTGTTGTGGATGGAAAATGTGATGTTGATGAGAAATCTTTTGACGGAGAGACTTTCCCAGTTCCCAAACTTAAAGATTCTCTTGTTTGGGCTGGTACCATCAACTTAAATGGTATTATATCTTCTTCCTTCCTTCCCCGTATAACTTTTTGAAATAGTTATATAATTAAAATTCCAACTCATCTATACCTTAAGGGGTAGTTTGGAAAGAAGTTAATGATTATTCCTTCTCTTTTTGTAGGTTATATTAGTGTTCAAACCACTGTTGTTGCTGAGGACTGCGTGGTGGCAAAGATGGCTGAGTTTGTAGAAGAAGCTCAAAACAACAAATCAAAAACTCAGAGATTCATTGATGAATGTGCCAAATATTACACTCCAGGTACCCCTTACACTTGTTATCCCAAAACCATCACTTTGGGATTCAAGCTCAAAAACACAGCATGTTTTTGTATGCAGCTGTAGTTGTCATATCAGCTTGTGTAGCAGCCATTCCAGCTGCATTCCGAGTTCACAATCTCAGCCACTGGTTTCACTTAGCTCTGGTGGTCTTAGTGAGTGCATGTCCCTGTGCCCTTATCCTCTCCACCCCGGTCGCCGCCTTCTGCGCCCTCACCAAGGCCGCCATGGCCGGTGTTCTCATCAAAGGCAGCGACCACCTTGAAGTTCTTGCAAAGATTAAGGTTGTAGCCTTCGACAAGACAGGCACAATCACTAGAGGTGAATTTGTGGTAACACATTTTCAAGCTCTACGAGATGATATCAGCTTCCACACCTTACTTCATTGGTAATTGCACAAAAAACTTCTCACCCAATTCGTAATTCATTGAAATATTTCATTTCATCGATTGAAGTGTTTATGTTGAAATAAGGGTTTCAAGCATTGAGAGCAAATCAAGCCATCCAATGGCAGCTGCCCTTGTCAACCATGCAAAGCTGCTTTCTATTGATATTAAACCTGAAAAAGTTGAGGAATTTGAGAACTTTCCAGGAGAAGGTGTTCGTGGGAAGATTGATGGGAAGGATATTTACATTGGAAGTACAAAAATTGCTGCCCGAGCTGGCTGCTCGTCAGGTTTATGATCAAACCCTCTTTATCTCCAAACATGTTTTCTGTTAGGAATCACGACCCTCCACAATAGTATGATACTGTCCACTTTGACCTTATCCTCCCTTCGAACAAAGTACACTATAGAGTCTCCCCTAAGGCCTATGCATAATCGAGCCCTAGAACAGCCTCCCCTTAATCGAGGCTCAACTCCTTTTCTAGAGTCCTTGAACAAAGTACACCCTTTGTTCGACACTTGAGTTACTTTTTATTACACCTTCGAGCCTATGAAGACTGTCTTCCTCACTTCTAAATCCACGATCATTCCTTGAGGATCAATGCGAGACTTTGACAAAGTTATAGAGCATCCTCTGAAGTTCTGAACAACCTTCTCTTAATCGATGCTTGATCTTCACTGGAGTCCTCCAACAAAGTATGTTCGACACTTGAGTCATTTTGACTACACCTGAGGCTCTTCTTATGTTCCGACACTTGAGAATTCTGCATGACTAAAAAAGGACATAGCTCTGAGGAATCATACCTCCATGGAGATGTATGATCCTTACTAGACATAAGCTCTCATTTAACGCTTCCCAAAATCCTCAACAGTTTCATGATCTTAATATAAACTTAATTTCTTCAGCTATGAAGTTAGAGGAGGAAATGAAACAAGGCCAAACCCTTGGATATGTATTTTGTGAGGAGATGGCTGTGGGATCCTTTGGTCTTTCGGATTCTTGTCGGTCAGGAGCTAAAGAAGGCATGGAGGAGCTCAAGAGTCTTGGCATTAAAACAGCTATGCTCACTGGGGACTGTAGTGCAGCTGCCATGCACGCCCAACAACAGGTTTTGGTTCATATTTTTGTTAAGTGAAATTAGAATCATTCAACATTCATAACGATGATGAGTTGTTTGCAGCTGGAGAACGCGGTTGATGTAATCCATTCAGAGCTTCTTCCAAAGGAGAAAGCAAATGTGATAAAAGAATTCAAAAGGGATTATGGAGCAACCGCCATGGTTGGCGATGGCTTAAATGATACCCCAGCATTAGCCACAGCTGATATTGGCATATCAATGGGCATTTCTGGTTCACCTCTCGCTACTCAAACTGGAAATGTCATCTTAATGTCAAATGACATCACCAAAATCCCACAAGCCATCAAACTAGCTAGGAAATCTCATGCAAAAGTTGTTCAAAATGTCATTTTGTCAATCGCTACCAAGATTGCAATCCTTGGCTTGGCCATCGCCGGACATCCGCTCGTTTGGGCGGCCGTTCTCGCCGATGTCGGTACCTGCCTATTGGTCATCTTGAACAGCATGCTTCTGTTGAGAGGAAGTGATCATAAACATGGGAACAAATGTTGCAAATCTTCCAAGCTATGCTCGACGAAACATGGACGATGTGATGAAAGTAACACTCGACCGTCTCTCATCATCATCATCACGATCATCGATGCCATGTCGTCGATGATAAATCGCCTAGTCGAGATAATCACAATGGAGATTGTGGGGGTAAATTTCATCACAATCATTCAAATAATGGGAATTGTTCAAAGAAAGTGGGTGAACCCAATTGTAATTGCCACTCACATCATGTAG

mRNA sequence

AATGGTGGGTGAGGTGGCAATGGAGAAGAACAAGAAAGTAAGGAGAGAGAACAAATTTGAAAGAAGCCATTTTGATGTGTTGGGAATTTGCTGTTCTTCAGAAGTTCCTCTCATTGAAAACATCCTCAAACCCCTCGAAGGAATCAAACAAATCTCTGTTATTGTCCCGACAAGAACTCTCATTGTCGTCCATGATTCCCTCCTCGTTTCTCAGCTTCAAATCGTGAAAGCACTAAACGAAGCAAGATTGGAGGCAAATGTACAATTAAAAGGAAAGGGAATTAGCAAGAAGAAATGGCCAAGTCCTTTTGCAATAGCCAGTGGGTTGCTTCTAACAGCATCCTTTTTGAAGTATGTCTACCACCCATTGCGGTGGCTGGCTGTGGCTGCGGTGGCCGCCGGCATCTTCCCCATTCTCCTAAAAGCCATCTCCGCGGTTCGCCATCTGAGGATCGATGTCAACATTCTTGCCATTATTGCGGTGGTGGGGACAATGAGTATGAATGACTACATGGAAGGTGGGAGCATAGTGTTTTTGTTTTCCATTGCTGAATGGCTTGAGTCAAGAGCAAGCCACAAGGCGAATGCTGCAATGTGGTCGCTGATGAGCCTGGCCCCTCAAAAGGCCACCATAGCTGAAACTGGAGAGGTTGTGGAGGTTAAAGAGGTGAAATTGAAGAGTGTGTTGGCAGTGAAGGGTGGTGAAGTTATTCCCATTGATGGCATTGTTGTGGATGGAAAATGTGATGTTGATGAGAAATCTTTTGACGGAGAGACTTTCCCAGTTCCCAAACTTAAAGATTCTCTTGTTTGGGCTGGTACCATCAACTTAAATGGTTATATTAGTGTTCAAACCACTGTTGTTGCTGAGGACTGCGTGGTGGCAAAGATGGCTGAGTTTGTAGAAGAAGCTCAAAACAACAAATCAAAAACTCAGAGATTCATTGATGAATGTGCCAAATATTACACTCCAGCTGTAGTTGTCATATCAGCTTGTGTAGCAGCCATTCCAGCTGCATTCCGAGTTCACAATCTCAGCCACTGGTTTCACTTAGCTCTGGTGGTCTTAGTGAGTGCATGTCCCTGTGCCCTTATCCTCTCCACCCCGGTCGCCGCCTTCTGCGCCCTCACCAAGGCCGCCATGGCCGGTGTTCTCATCAAAGGCAGCGACCACCTTGAAGTTCTTGCAAAGATTAAGGTTGTAGCCTTCGACAAGACAGGCACAATCACTAGAGGTGAATTTGTGGTAACACATTTTCAAGCTCTACGAGATGATATCAGCTTCCACACCTTACTTCATTGGGTTTCAAGCATTGAGAGCAAATCAAGCCATCCAATGGCAGCTGCCCTTGTCAACCATGCAAAGCTGCTTTCTATTGATATTAAACCTGAAAAAGTTGAGGAATTTGAGAACTTTCCAGGAGAAGGTGTTCGTGGGAAGATTGATGGGAAGGATATTTACATTGGAAGTACAAAAATTGCTGCCCGAGCTGGCTGCTCGTCAGCTATGAAGTTAGAGGAGGAAATGAAACAAGGCCAAACCCTTGGATATGTATTTTGTGAGGAGATGGCTGTGGGATCCTTTGGTCTTTCGGATTCTTGTCGGTCAGGAGCTAAAGAAGGCATGGAGGAGCTCAAGAGTCTTGGCATTAAAACAGCTATGCTCACTGGGGACTGTAGTGCAGCTGCCATGCACGCCCAACAACAGCTGGAGAACGCGGTTGATGTAATCCATTCAGAGCTTCTTCCAAAGGAGAAAGCAAATGTGATAAAAGAATTCAAAAGGGATTATGGAGCAACCGCCATGGTTGGCGATGGCTTAAATGATACCCCAGCATTAGCCACAGCTGATATTGGCATATCAATGGGCATTTCTGGTTCACCTCTCGCTACTCAAACTGGAAATGTCATCTTAATGTCAAATGACATCACCAAAATCCCACAAGCCATCAAACTAGCTAGGAAATCTCATGCAAAAGTTGTTCAAAATGTCATTTTGTCAATCGCTACCAAGATTGCAATCCTTGGCTTGGCCATCGCCGGACATCCGCTCGTTTGGGCGGCCGTTCTCGCCGATGTCGGTACCTGCCTATTGGTCATCTTGAACAGCATGCTTCTGTTGAGAGGAAGTGATCATAAACATGGGAACAAATGTTGCAAATCTTCCAAGCTATGCTCGACGAAACATGGACGATGTGATGAAAGTAACACTCGACCGTCTCTCATCATCATCATCACGATCATCGATGCCATGTCGTCGATGATAAATCGCCTAGTCGAGATAATCACAATGGAGATTGTGGGGGTAAATTTCATCACAATCATTCAAATAATGGGAATTGTTCAAAGAAAGTGGGTGAACCCAATTGTAATTGCCACTCACATCATGTAG

Coding sequence (CDS)

ATGGTGGGTGAGGTGGCAATGGAGAAGAACAAGAAAGTAAGGAGAGAGAACAAATTTGAAAGAAGCCATTTTGATGTGTTGGGAATTTGCTGTTCTTCAGAAGTTCCTCTCATTGAAAACATCCTCAAACCCCTCGAAGGAATCAAACAAATCTCTGTTATTGTCCCGACAAGAACTCTCATTGTCGTCCATGATTCCCTCCTCGTTTCTCAGCTTCAAATCGTGAAAGCACTAAACGAAGCAAGATTGGAGGCAAATGTACAATTAAAAGGAAAGGGAATTAGCAAGAAGAAATGGCCAAGTCCTTTTGCAATAGCCAGTGGGTTGCTTCTAACAGCATCCTTTTTGAAGTATGTCTACCACCCATTGCGGTGGCTGGCTGTGGCTGCGGTGGCCGCCGGCATCTTCCCCATTCTCCTAAAAGCCATCTCCGCGGTTCGCCATCTGAGGATCGATGTCAACATTCTTGCCATTATTGCGGTGGTGGGGACAATGAGTATGAATGACTACATGGAAGGTGGGAGCATAGTGTTTTTGTTTTCCATTGCTGAATGGCTTGAGTCAAGAGCAAGCCACAAGGCGAATGCTGCAATGTGGTCGCTGATGAGCCTGGCCCCTCAAAAGGCCACCATAGCTGAAACTGGAGAGGTTGTGGAGGTTAAAGAGGTGAAATTGAAGAGTGTGTTGGCAGTGAAGGGTGGTGAAGTTATTCCCATTGATGGCATTGTTGTGGATGGAAAATGTGATGTTGATGAGAAATCTTTTGACGGAGAGACTTTCCCAGTTCCCAAACTTAAAGATTCTCTTGTTTGGGCTGGTACCATCAACTTAAATGGTTATATTAGTGTTCAAACCACTGTTGTTGCTGAGGACTGCGTGGTGGCAAAGATGGCTGAGTTTGTAGAAGAAGCTCAAAACAACAAATCAAAAACTCAGAGATTCATTGATGAATGTGCCAAATATTACACTCCAGCTGTAGTTGTCATATCAGCTTGTGTAGCAGCCATTCCAGCTGCATTCCGAGTTCACAATCTCAGCCACTGGTTTCACTTAGCTCTGGTGGTCTTAGTGAGTGCATGTCCCTGTGCCCTTATCCTCTCCACCCCGGTCGCCGCCTTCTGCGCCCTCACCAAGGCCGCCATGGCCGGTGTTCTCATCAAAGGCAGCGACCACCTTGAAGTTCTTGCAAAGATTAAGGTTGTAGCCTTCGACAAGACAGGCACAATCACTAGAGGTGAATTTGTGGTAACACATTTTCAAGCTCTACGAGATGATATCAGCTTCCACACCTTACTTCATTGGGTTTCAAGCATTGAGAGCAAATCAAGCCATCCAATGGCAGCTGCCCTTGTCAACCATGCAAAGCTGCTTTCTATTGATATTAAACCTGAAAAAGTTGAGGAATTTGAGAACTTTCCAGGAGAAGGTGTTCGTGGGAAGATTGATGGGAAGGATATTTACATTGGAAGTACAAAAATTGCTGCCCGAGCTGGCTGCTCGTCAGCTATGAAGTTAGAGGAGGAAATGAAACAAGGCCAAACCCTTGGATATGTATTTTGTGAGGAGATGGCTGTGGGATCCTTTGGTCTTTCGGATTCTTGTCGGTCAGGAGCTAAAGAAGGCATGGAGGAGCTCAAGAGTCTTGGCATTAAAACAGCTATGCTCACTGGGGACTGTAGTGCAGCTGCCATGCACGCCCAACAACAGCTGGAGAACGCGGTTGATGTAATCCATTCAGAGCTTCTTCCAAAGGAGAAAGCAAATGTGATAAAAGAATTCAAAAGGGATTATGGAGCAACCGCCATGGTTGGCGATGGCTTAAATGATACCCCAGCATTAGCCACAGCTGATATTGGCATATCAATGGGCATTTCTGGTTCACCTCTCGCTACTCAAACTGGAAATGTCATCTTAATGTCAAATGACATCACCAAAATCCCACAAGCCATCAAACTAGCTAGGAAATCTCATGCAAAAGTTGTTCAAAATGTCATTTTGTCAATCGCTACCAAGATTGCAATCCTTGGCTTGGCCATCGCCGGACATCCGCTCGTTTGGGCGGCCGTTCTCGCCGATGTCGGTACCTGCCTATTGGTCATCTTGAACAGCATGCTTCTGTTGAGAGGAAGTGATCATAAACATGGGAACAAATGTTGCAAATCTTCCAAGCTATGCTCGACGAAACATGGACGATGTGATGAAAGTAACACTCGACCGTCTCTCATCATCATCATCACGATCATCGATGCCATGTCGTCGATGATAAATCGCCTAGTCGAGATAATCACAATGGAGATTGTGGGGGTAAATTTCATCACAATCATTCAAATAATGGGAATTGTTCAAAGAAAGTGGGTGAACCCAATTGTAATTGCCACTCACATCATGTAG

Protein sequence

MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKSSKLCSTKHGRCDESNTRPSLIIIITIIDAMSSMINRLVEIITMEIVGVNFITIIQIMGIVQRKWVNPIVIATHIM
BLAST of Carg23927 vs. NCBI nr
Match: XP_022929619.1 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1392.9 bits (3604), Expect = 0.0e+00
Identity = 731/740 (98.78%), Postives = 731/740 (98.78%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVG MSMNDYME GSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEV LKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKS  GETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQ 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVT FQ
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 480
           ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLS DIKPEKVEEFENFPGEGVRGK
Sbjct: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGK 480

Query: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540
           IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE
Sbjct: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540

Query: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600
           GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT
Sbjct: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600

Query: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660
           AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV
Sbjct: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660

Query: 661 VQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720
           VQNVILSI TKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK
Sbjct: 661 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720

Query: 721 SSKLCSTKHGRCDESNTRPS 741
           SSKLCSTKHGRCD SNTRPS
Sbjct: 721 SSKLCSTKHGRCDGSNTRPS 740

BLAST of Carg23927 vs. NCBI nr
Match: XP_022929620.1 (cadmium/zinc-transporting ATPase HMA2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1392.9 bits (3604), Expect = 0.0e+00
Identity = 731/740 (98.78%), Postives = 731/740 (98.78%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVG MSMNDYME GSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEV LKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKS  GETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQ 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVT FQ
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 480
           ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLS DIKPEKVEEFENFPGEGVRGK
Sbjct: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGK 480

Query: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540
           IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE
Sbjct: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540

Query: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600
           GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT
Sbjct: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600

Query: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660
           AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV
Sbjct: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660

Query: 661 VQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720
           VQNVILSI TKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK
Sbjct: 661 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720

Query: 721 SSKLCSTKHGRCDESNTRPS 741
           SSKLCSTKHGRCD SNTRPS
Sbjct: 721 SSKLCSTKHGRCDGSNTRPS 740

BLAST of Carg23927 vs. NCBI nr
Match: XP_023545589.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 723/740 (97.70%), Postives = 728/740 (98.38%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKN KV RENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNMKVTRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           IVVHDSLLVSQLQI KALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIAKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGT+SMNDYME GSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTISMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKS  GETFPVPKLK SLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKGSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAA RVHNL+HWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAALRVHNLTHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQ 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVT+F+
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFE 420

Query: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 480
           ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK
Sbjct: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 480

Query: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540
           IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFC EMAVGSFGLSDSCRSGAKE
Sbjct: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCGEMAVGSFGLSDSCRSGAKE 540

Query: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600
           GMEELKS GIKTAMLTGDC+AAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT
Sbjct: 541 GMEELKSFGIKTAMLTGDCNAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600

Query: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660
           AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV
Sbjct: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660

Query: 661 VQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720
           VQNVILSI TKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK
Sbjct: 661 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720

Query: 721 SSKLCSTKHGRCDESNTRPS 741
           SSKLCSTKHGRCD SNTRPS
Sbjct: 721 SSKLCSTKHGRCDGSNTRPS 740

BLAST of Carg23927 vs. NCBI nr
Match: XP_022929621.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1374.8 bits (3557), Expect = 0.0e+00
Identity = 724/740 (97.84%), Postives = 725/740 (97.97%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKK    NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKK----NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           IVVHDSLLVSQLQIVKALNEARLEA VQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEAKVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYME GSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEV LKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKS  GETFPVPKLKDSLVWAGTINLNGYISVQTT VAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQ 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVT FQ
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 480
           ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLS DIKPEKVEEFENFPGEGVRGK
Sbjct: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGK 480

Query: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540
           IDGKDIYIGSTKIAARAGCSSAMKLEEEMK GQTLGYVFCEEMAVGSFGLSDSCRSGAKE
Sbjct: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540

Query: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600
           GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKAN+IKEFKRDYGAT
Sbjct: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 600

Query: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660
           AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV
Sbjct: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660

Query: 661 VQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720
           VQNVILSI TKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK
Sbjct: 661 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720

Query: 721 SSKLCSTKHGRCDESNTRPS 741
           SSKLCSTKHGRCD SNTRPS
Sbjct: 721 SSKLCSTKHGRCDGSNTRPS 736

BLAST of Carg23927 vs. NCBI nr
Match: XP_022929622.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1374.8 bits (3557), Expect = 0.0e+00
Identity = 724/740 (97.84%), Postives = 725/740 (97.97%), Query Frame = 0

Query: 1   MVGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60
           MVGEVAMEKNKK    NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL
Sbjct: 1   MVGEVAMEKNKK----NKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTL 60

Query: 61  IVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120
           IVVHDSLLVSQLQIVKALNEARLEA VQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY
Sbjct: 61  IVVHDSLLVSQLQIVKALNEARLEAKVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVY 120

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYME GSIVFLF
Sbjct: 121 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLF 180

Query: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPID 240
           SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEV LKSVLAVKGGEVIPID
Sbjct: 181 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 240

Query: 241 GIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 300
           GIVVDGKCDVDEKS  GETFPVPKLKDSLVWAGTINLNGYISVQTT VAEDCVVAKMAEF
Sbjct: 241 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEF 300

Query: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC
Sbjct: 301 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 360

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQ 420
           PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVT FQ
Sbjct: 361 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 420

Query: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 480
           ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLS DIKPEKVEEFENFPGEGVRGK
Sbjct: 421 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGK 480

Query: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540
           IDGKDIYIGSTKIAARAGCSSAMKLEEEMK GQTLGYVFCEEMAVGSFGLSDSCRSGAKE
Sbjct: 481 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 540

Query: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 600
           GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKAN+IKEFKRDYGAT
Sbjct: 541 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 600

Query: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660
           AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV
Sbjct: 601 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 660

Query: 661 VQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720
           VQNVILSI TKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK
Sbjct: 661 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 720

Query: 721 SSKLCSTKHGRCDESNTRPS 741
           SSKLCSTKHGRCD SNTRPS
Sbjct: 721 SSKLCSTKHGRCDGSNTRPS 736

BLAST of Carg23927 vs. TAIR10
Match: AT4G30110.1 (heavy metal atpase 2)

HSP 1 Score: 955.3 bits (2468), Expect = 2.4e-278
Identity = 482/709 (67.98%), Postives = 592/709 (83.50%), Query Frame = 0

Query: 18  KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSLLVSQLQIVKA 77
           K  +S+FDVLGICC+SEVPLIENIL  ++G+K+ SVIVP+RT+IVVHD+L++SQ QIVKA
Sbjct: 5   KMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKA 64

Query: 78  LNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFP 137
           LN+A+LEANV++ G+   K KWPSPFA+ SG+LL  SF KY+Y P RWLAVAAV AGI+P
Sbjct: 65  LNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYP 124

Query: 138 ILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLESRASHKANAA 197
           IL KA++++   RID+NIL ++ V  T+ M DY E   +VFLF+IAEWL+SRAS+KA+A 
Sbjct: 125 ILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAV 184

Query: 198 MWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSFDG 257
           M SLMSLAPQKA IAETGE VEV E+K  +V+AVK GE IPIDG+VVDG C+VDEK+  G
Sbjct: 185 MQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTG 244

Query: 258 ETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDE 317
           E FPVPKLKDS VWAGTINLNGYI+V TT +AEDCVVAKMA+ VEEAQN+K++TQRFID+
Sbjct: 245 EAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDK 304

Query: 318 CAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 377
           C+KYYTPA+++IS C  AIP A +VHNL HW HLALVVLVSACPC LILSTPVA FCALT
Sbjct: 305 CSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALT 364

Query: 378 KAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDISFHTLLHWVSS 437
           KAA +G+LIKG+D+LE LAKIK+VAFDKTGTITRGEF+V  FQ+L +DIS  +LL+WVSS
Sbjct: 365 KAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLLYWVSS 424

Query: 438 IESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIYIGSTKIAARA 497
            ESKSSHPMAAA+V++A+ +S++ KPE VE+++NFPGEG+ GKIDGK++YIG+ +IA+RA
Sbjct: 425 TESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRA 484

Query: 498 GCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKSLGIKTAMLTG 557
           GC S   ++ + K G+T+GYV+  E   G F LSD+CRSG  + M+ELKSLGIK AMLTG
Sbjct: 485 GCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKIAMLTG 544

Query: 558 DCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGLNDTPALATAD 617
           D  AAAMHAQ+QL NA+D++ +ELLP++K+ +IK+ KR+ G TAMVGDGLND PALATAD
Sbjct: 545 DNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPALATAD 604

Query: 618 IGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILSIATKIAILGL 677
           IGISMG+SGS LAT+TGN+ILMSNDI +IPQAIKLA+++  KVV+NV++SI  K AIL L
Sbjct: 605 IGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAILAL 664

Query: 678 AIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKSSKLCS 727
           A AGHPL+WAAVLADVGTCLLVILNSMLLL    HK GNKC + S   S
Sbjct: 665 AFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESSSSS 712

BLAST of Carg23927 vs. TAIR10
Match: AT2G19110.1 (heavy metal atpase 4)

HSP 1 Score: 946.4 bits (2445), Expect = 1.1e-275
Identity = 485/724 (66.99%), Postives = 601/724 (83.01%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSL 67
           E+ KKV+   K ++S+FDVLGICC+SEVP+IENILK L+G+K+ SVIVP+RT+IVVHDSL
Sbjct: 8   EEKKKVK---KLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSL 67

Query: 68  LVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
           L+S  QI KALNEARLEANV++ G+   K KWPSPFA+ SGLLL  SFLK+VY PLRWLA
Sbjct: 68  LISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLA 127

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLE 187
           VAAVAAGI+PIL KA ++++  RID+NIL II V+ T++M D+ME  ++VFLF+I++WLE
Sbjct: 128 VAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLE 187

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGK 247
           +RAS+KA + M SLMSLAPQKA IAETGE VEV EVK+ +V+AVK GE IPIDGIVVDG 
Sbjct: 188 TRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGN 247

Query: 248 CDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           C+VDEK+  GE FPVPK +DS VWAGTINLNGYI V+TT +A DCVVAKMA+ VEEAQ++
Sbjct: 248 CEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSS 307

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           K+K+QR ID+C++YYTPA++++SACVA +P   +VHNL HWFHLALVVLVS CPC LILS
Sbjct: 308 KTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILS 367

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDIS 427
           TPVA FCALTKAA +G+LIK +D+L+ L+KIK+VAFDKTGTITRGEF+V  F++L  DI+
Sbjct: 368 TPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDIN 427

Query: 428 FHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIY 487
             +LL+WVSS+ESKSSHPMAA +V++AK +S++ +PE+VE+++NFPGEG+ GKIDG DI+
Sbjct: 428 LRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIF 487

Query: 488 IGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKS 547
           IG+ KIA+RAGCS+  ++E + K G+T+GYV+  E   G F LSD+CRSG  + M ELKS
Sbjct: 488 IGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKS 547

Query: 548 LGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGL 607
           LGIKTAMLTGD  AAAMHAQ+QL N +DV+H +LLP++K+ +I+EFK++ G TAMVGDG+
Sbjct: 548 LGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE-GPTAMVGDGV 607

Query: 608 NDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILS 667
           ND PALATADIGISMGISGS LATQTGN+ILMSNDI +IPQA+KLAR++  KVV+NV LS
Sbjct: 608 NDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLS 667

Query: 668 IATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCC---KSSKL 727
           I  K  IL LA AGHPL+WAAVL DVGTCLLVI NSMLLLR    K GNK C    +SKL
Sbjct: 668 IILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLR-EKKKIGNKKCYRASTSKL 726

Query: 728 CSTK 729
              K
Sbjct: 728 NGRK 726

BLAST of Carg23927 vs. TAIR10
Match: AT4G30120.1 (heavy metal atpase 3)

HSP 1 Score: 648.7 bits (1672), Expect = 4.8e-186
Identity = 328/542 (60.52%), Postives = 424/542 (78.23%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSL 67
           E++KK+      + S+FDV+GICCSSEV ++ N+L+ ++G+K+ SVIVP+RT+IVVHD+ 
Sbjct: 5   EESKKM----NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHDTF 64

Query: 68  LVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
           L+S LQIVKALN+ARLEA+V+  G+   K +WPSPFAI SG+LL  SF KY Y PL WLA
Sbjct: 65  LISPLQIVKALNQARLEASVRPYGETSLKSQWPSPFAIVSGVLLVLSFFKYFYSPLEWLA 124

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLE 187
           + AV AG+FPIL KA+++V   R+D+N L +IAV+ T+ M D+ E  +IVFLFS+A+WLE
Sbjct: 125 IVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVADWLE 184

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGK 247
           S A+HKA+  M SLMSLAP+KA IA+TG  V+V EV + +V++VK GE IPIDG+VVDG 
Sbjct: 185 SSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVVDGS 244

Query: 248 CDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           CDVDEK+  GE+FPV K ++S V A TINLNGYI V+TT +A DCVVAKM + VEEAQ +
Sbjct: 245 CDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEAQKS 304

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           ++KTQRFID+C++YYTPAVVV +AC A IP   +V +LSHWFHLALVVLVS CPC LILS
Sbjct: 305 QTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGLILS 364

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDIS 427
           TPVA FCALTKAA +G LIK  D LE LAKIK+VAFDKTGTIT+ EF+V+ F++L   I+
Sbjct: 365 TPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSPSIN 424

Query: 428 FHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIY 487
            H LL+WVSSIE KSSHPMAAAL+++A+ +S++ KP+ VE F+NFPGEGV G+IDG+DIY
Sbjct: 425 LHKLLYWVSSIECKSSHPMAAALIDYARSVSVEPKPDIVENFQNFPGEGVYGRIDGQDIY 484

Query: 488 IGSTKIAARAGC--SSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEEL 547
           IG+ +IA RAGC   +   +E  MK+G+T+GY++      GSF L D CR G  + ++EL
Sbjct: 485 IGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGCRYGVAQALKEL 542

BLAST of Carg23927 vs. TAIR10
Match: AT4G33520.2 (P-type ATP-ase 1)

HSP 1 Score: 229.2 bits (583), Expect = 9.0e-60
Identity = 176/588 (29.93%), Postives = 291/588 (49.49%), Query Frame = 0

Query: 154 NILAIIAVVGTMSMNDYMEGGSIVFLF-SIAEWLESRASHKANAAMWSLMSLAPQKATIA 213
           ++ ++ A++  +    + E   ++  F  +   LE RA  KA + M  L+S+ P KA + 
Sbjct: 331 SVSSLAAMIPKLGWKTFFEEPVMLIAFVLLGRNLEQRAKIKATSDMTGLLSVLPSKARLL 390

Query: 214 ETGEV------VEVKEVKLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSFDGETFPVPKLK 273
             G++      V    + +  ++ +  G+ +P DG+V  G+  +DE SF GE  PV K  
Sbjct: 391 LDGDLQNSTVEVPCNSLSVGDLVVILPGDRVPADGVVKSGRSTIDESSFTGEPLPVTKES 450

Query: 274 DSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDECAKYYTPAV 333
            S V AG+INLNG ++V+      +  V  +   VEEAQ+ ++  Q+ +D+ A  +T  V
Sbjct: 451 GSQVAAGSINLNGTLTVEVHRSGGETAVGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGV 510

Query: 334 VVISACVAAIPAAFRVHNLSHWFH----------LALVVLVSACPCALILSTPVAAFCAL 393
           + +SA        F  H L    H          L+  VLV ACPCAL L+TP A     
Sbjct: 511 MALSAATFTFWNLFGAHVLPSALHNGSPMSLALQLSCSVLVVACPCALGLATPTAMLVGT 570

Query: 394 TKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHF-------QALRDDISFH 453
           +  A  G+L++G D LE  + +  V FDKTGT+T+G  VVT           L D  S  
Sbjct: 571 SLGARRGLLLRGGDILEKFSLVDTVVFDKTGTLTKGHPVVTEVIIPENPRHNLNDTWSEV 630

Query: 454 TLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIYIG 513
            +L   +++ES ++HP+  A+V  A+  +      +   F   PG G    ++ K + +G
Sbjct: 631 EVLMLAAAVESNTTHPVGKAIVKAARARNCQTMKAEDGTFTEEPGSGAVAIVNNKRVTVG 690

Query: 514 STKIAARAGC--SSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKS 573
           + +   R G   +S + LEE     Q++ Y+  +          D  R  A + +E L  
Sbjct: 691 TLEWVKRHGATGNSLLALEEHEINNQSVVYIGVDNTLAAVIRFEDKVREDAAQVVENLTR 750

Query: 574 LGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGL 633
            GI   ML+GD   AA +    +    + + + + P EK N I E +++    AMVGDG+
Sbjct: 751 QGIDVYMLSGDKRNAANYVASVVGINHERVIAGVKPAEKKNFINELQKNKKIVAMVGDGI 810

Query: 634 NDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNV--- 693
           ND  ALA++++G++MG  G+  A++   V+LM N +T++  A++L+R++   V QN+   
Sbjct: 811 NDAAALASSNVGVAMG-GGAGAASEVSPVVLMGNRLTQLLDAMELSRQTMKTVKQNLWWA 870

Query: 694 ----ILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLR 709
               I+ I     +L L + G  L  +   A +G   L ++ + LLLR
Sbjct: 871 FGYNIVGIPIAAGVL-LPLTGTMLTPSMAGALMGVSSLGVMTNSLLLR 916

BLAST of Carg23927 vs. TAIR10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1))

HSP 1 Score: 208.0 bits (528), Expect = 2.2e-53
Identity = 180/606 (29.70%), Postives = 299/606 (49.34%), Query Frame = 0

Query: 153 VNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLESRASHKANAAMWSLMSLAPQKA--- 212
           V  L   AV G  S   +     ++    + ++LES A  K + AM  L+ L P  A   
Sbjct: 386 VGALLYGAVTGFWSPTYFDASAMLITFVLLGKYLESLAKGKTSDAMKKLVQLTPATAILL 445

Query: 213 TIAETGEVVEVKEVKLKSV-----LAVKGGEVIPIDGIVVDGKCDVDEKSFDGETFPVPK 272
           T  + G++V  +E+    +     L V  G  IP DG+VV G   V+E    GE+ PV K
Sbjct: 446 TEGKGGKLVGEREIDALLIQPGDTLKVHPGAKIPADGVVVWGSSYVNESMVTGESVPVSK 505

Query: 273 LKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDECAKYYTP 332
             DS V  GTIN++G + ++ T V  D V++++   VE AQ +K+  Q+F D  A  + P
Sbjct: 506 EVDSPVIGGTINMHGALHMKATKVGSDAVLSQIISLVETAQMSKAPIQKFADYVASIFVP 565

Query: 333 AVVV----------ISACVAAIPAAFRVHNLSHW---FHLALVVLVSACPCALILSTPVA 392
            V+           I   V A P  +   N +H+      ++ V+              A
Sbjct: 566 VVITLALFTLVGWSIGGAVGAYPDEWLPENGTHFVFSLMFSISVVXXXXXXXXXXXXXXA 625

Query: 393 AFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDISFHTL 452
              A    A  GVLIKG D LE   K+K V FDKTGT+T+G+  VT  +    ++     
Sbjct: 626 VMVATGVGATNGVLIKGGDALEKAHKVKYVIFDKTGTLTQGKATVTTTKVF-SEMDRGEF 685

Query: 453 LHWVSSIESKSSHPMAAALVNHAKLLS-IDIKPEKVE----------------EFENFPG 512
           L  V+S E+ S HP+A A+V +A+     D   E  E                +F   PG
Sbjct: 686 LTLVASAEASSEHPLAKAIVAYARHFHFFDESTEDGETNNKDLQNSGWLLDTSDFSALPG 745

Query: 513 EGVRGKIDGKDIYIGSTKIAARAGCS---SAMKLEEEMKQ-GQTLGYVFCEEMAVGSFGL 572
           +G++  ++ K I +G+ K+ +    +      K  E++++ G+T   V      VG  G+
Sbjct: 746 KGIQCLVNEKMILVGNRKLMSENAINIPDHVEKFVEDLEESGKTGVIVAYNGKLVGVMGI 805

Query: 573 SDSCRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVI 632
           +D  +  A   +E L  +G++  M+TGD    A    +++   ++ + +E++P  KA+VI
Sbjct: 806 ADPLKREAALVVEGLLRMGVRPIMVTGDNWRTARAVAKEV--GIEDVRAEVMPAGKADVI 865

Query: 633 KEFKRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAI 692
           +  ++D    AMVGDG+ND+PALA AD+G+++G +G+ +A +  + +LM N++  +  AI
Sbjct: 866 RSLQKDGSTVAMVGDGINDSPALAAADVGMAIG-AGTDVAIEAADYVLMRNNLEDVITAI 925

Query: 693 KLARKSHAKVVQNVILSIATKIAILGLAIAG--HPLV------WAAVLADVGTCLLVILN 709
            L+RK+  ++  N + ++A  +  + +A AG   P++      WAA      + + V+ +
Sbjct: 926 DLSRKTLTRIRLNYVFAMAYNVVSIPIA-AGVFFPVLRVQLPPWAAGACMALSSVSVVCS 985

BLAST of Carg23927 vs. Swiss-Prot
Match: sp|Q9SZW4|HMA2_ARATH (Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE=2 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 4.3e-277
Identity = 482/709 (67.98%), Postives = 592/709 (83.50%), Query Frame = 0

Query: 18  KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSLLVSQLQIVKA 77
           K  +S+FDVLGICC+SEVPLIENIL  ++G+K+ SVIVP+RT+IVVHD+L++SQ QIVKA
Sbjct: 5   KMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKA 64

Query: 78  LNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFP 137
           LN+A+LEANV++ G+   K KWPSPFA+ SG+LL  SF KY+Y P RWLAVAAV AGI+P
Sbjct: 65  LNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYP 124

Query: 138 ILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLESRASHKANAA 197
           IL KA++++   RID+NIL ++ V  T+ M DY E   +VFLF+IAEWL+SRAS+KA+A 
Sbjct: 125 ILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAV 184

Query: 198 MWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSFDG 257
           M SLMSLAPQKA IAETGE VEV E+K  +V+AVK GE IPIDG+VVDG C+VDEK+  G
Sbjct: 185 MQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTG 244

Query: 258 ETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDE 317
           E FPVPKLKDS VWAGTINLNGYI+V TT +AEDCVVAKMA+ VEEAQN+K++TQRFID+
Sbjct: 245 EAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDK 304

Query: 318 CAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 377
           C+KYYTPA+++IS C  AIP A +VHNL HW HLALVVLVSACPC LILSTPVA FCALT
Sbjct: 305 CSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALT 364

Query: 378 KAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDISFHTLLHWVSS 437
           KAA +G+LIKG+D+LE LAKIK+VAFDKTGTITRGEF+V  FQ+L +DIS  +LL+WVSS
Sbjct: 365 KAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLLYWVSS 424

Query: 438 IESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIYIGSTKIAARA 497
            ESKSSHPMAAA+V++A+ +S++ KPE VE+++NFPGEG+ GKIDGK++YIG+ +IA+RA
Sbjct: 425 TESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRA 484

Query: 498 GCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKSLGIKTAMLTG 557
           GC S   ++ + K G+T+GYV+  E   G F LSD+CRSG  + M+ELKSLGIK AMLTG
Sbjct: 485 GCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKIAMLTG 544

Query: 558 DCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGLNDTPALATAD 617
           D  AAAMHAQ+QL NA+D++ +ELLP++K+ +IK+ KR+ G TAMVGDGLND PALATAD
Sbjct: 545 DNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPALATAD 604

Query: 618 IGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILSIATKIAILGL 677
           IGISMG+SGS LAT+TGN+ILMSNDI +IPQAIKLA+++  KVV+NV++SI  K AIL L
Sbjct: 605 IGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAILAL 664

Query: 678 AIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKSSKLCS 727
           A AGHPL+WAAVLADVGTCLLVILNSMLLL    HK GNKC + S   S
Sbjct: 665 AFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESSSSS 712

BLAST of Carg23927 vs. Swiss-Prot
Match: sp|O64474|HMA4_ARATH (Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 GN=HMA4 PE=1 SV=2)

HSP 1 Score: 946.4 bits (2445), Expect = 2.0e-274
Identity = 485/724 (66.99%), Postives = 601/724 (83.01%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSL 67
           E+ KKV+   K ++S+FDVLGICC+SEVP+IENILK L+G+K+ SVIVP+RT+IVVHDSL
Sbjct: 8   EEKKKVK---KLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSL 67

Query: 68  LVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
           L+S  QI KALNEARLEANV++ G+   K KWPSPFA+ SGLLL  SFLK+VY PLRWLA
Sbjct: 68  LISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLA 127

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLE 187
           VAAVAAGI+PIL KA ++++  RID+NIL II V+ T++M D+ME  ++VFLF+I++WLE
Sbjct: 128 VAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLE 187

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGK 247
           +RAS+KA + M SLMSLAPQKA IAETGE VEV EVK+ +V+AVK GE IPIDGIVVDG 
Sbjct: 188 TRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGN 247

Query: 248 CDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           C+VDEK+  GE FPVPK +DS VWAGTINLNGYI V+TT +A DCVVAKMA+ VEEAQ++
Sbjct: 248 CEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSS 307

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           K+K+QR ID+C++YYTPA++++SACVA +P   +VHNL HWFHLALVVLVS CPC LILS
Sbjct: 308 KTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILS 367

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDIS 427
           TPVA FCALTKAA +G+LIK +D+L+ L+KIK+VAFDKTGTITRGEF+V  F++L  DI+
Sbjct: 368 TPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDIN 427

Query: 428 FHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIY 487
             +LL+WVSS+ESKSSHPMAA +V++AK +S++ +PE+VE+++NFPGEG+ GKIDG DI+
Sbjct: 428 LRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIF 487

Query: 488 IGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKS 547
           IG+ KIA+RAGCS+  ++E + K G+T+GYV+  E   G F LSD+CRSG  + M ELKS
Sbjct: 488 IGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKS 547

Query: 548 LGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGL 607
           LGIKTAMLTGD  AAAMHAQ+QL N +DV+H +LLP++K+ +I+EFK++ G TAMVGDG+
Sbjct: 548 LGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE-GPTAMVGDGV 607

Query: 608 NDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILS 667
           ND PALATADIGISMGISGS LATQTGN+ILMSNDI +IPQA+KLAR++  KVV+NV LS
Sbjct: 608 NDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLS 667

Query: 668 IATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCC---KSSKL 727
           I  K  IL LA AGHPL+WAAVL DVGTCLLVI NSMLLLR    K GNK C    +SKL
Sbjct: 668 IILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLR-EKKKIGNKKCYRASTSKL 726

Query: 728 CSTK 729
              K
Sbjct: 728 NGRK 726

BLAST of Carg23927 vs. Swiss-Prot
Match: sp|P0CW78|HMA3B_ARATH (Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 1.4e-246
Identity = 442/717 (61.65%), Postives = 566/717 (78.94%), Query Frame = 0

Query: 8   EKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSL 67
           E++KK+      + S+FDV+GICCSSEV ++ N+L+ ++G+K+ SVIVP+RT+IVVHD+ 
Sbjct: 5   EESKKM----NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHDTF 64

Query: 68  LVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 127
           L+S LQIVKALN+ARLEA+V+  G+   K +WPSPFAI SG+LL  SF KY Y PL WLA
Sbjct: 65  LISPLQIVKALNQARLEASVRPYGETSLKSQWPSPFAIVSGVLLVLSFFKYFYSPLEWLA 124

Query: 128 VAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLE 187
           + AV AG+FPIL KA+++V   R+D+N L +IAV+ T+ M D+ E  +IVFLFS+A+WLE
Sbjct: 125 IVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVADWLE 184

Query: 188 SRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGK 247
           S A+HKA+  M SLMSLAP+KA IA+TG  V+V EV + +V++VK GE IPIDG+VVDG 
Sbjct: 185 SSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVVDGS 244

Query: 248 CDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNN 307
           CDVDEK+  GE+FPV K ++S V A TINLNGYI V+TT +A DCVVAKM + VEEAQ +
Sbjct: 245 CDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEAQKS 304

Query: 308 KSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILS 367
           ++KTQRFID+C++YYTPAVVV +AC A IP   +V +LSHWFHLALVVLVS CPC LILS
Sbjct: 305 QTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGLILS 364

Query: 368 TPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDIS 427
           TPVA FCALTKAA +G LIK  D LE LAKIK+VAFDKTGTIT+ EF+V+ F++L   I+
Sbjct: 365 TPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSPSIN 424

Query: 428 FHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIY 487
            H LL+WVSSIE KSSHPMAAAL+++A  +S++ KP+ VE F+NFPGEGV G+IDG+DIY
Sbjct: 425 LHKLLNWVSSIECKSSHPMAAALIDYAISVSVEPKPDIVENFQNFPGEGVYGRIDGQDIY 484

Query: 488 IGSTKIAARAGC--SSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEEL 547
           IG+ +IA RAGC   +   +E  MK+G+T+GY++      GSF L D CR G  + ++EL
Sbjct: 485 IGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGCRYGVAQALKEL 544

Query: 548 KSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGD 607
           KSLGI+TAMLTGD   AAM  Q+QLENA+D++HSELLP++KA +I +FK   G T MVGD
Sbjct: 545 KSLGIQTAMLTGDNQDAAMSTQEQLENALDIVHSELLPQDKARIIDDFKIQ-GPTMMVGD 604

Query: 608 GLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVI 667
           GLND PALA ADIGISMGISGS LAT+TG++ILMSNDI KIP+ ++LA++SH KV++NV+
Sbjct: 605 GLNDAPALAKADIGISMGISGSALATETGDIILMSNDIRKIPKGMRLAKRSHKKVIENVV 664

Query: 668 LSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKSS 723
           LS++ K AI+ L   G+PLVWAAVLAD GTCLLVILNSM+LLR  + +  + C +SS
Sbjct: 665 LSVSIKGAIMVLGFVGYPLVWAAVLADAGTCLLVILNSMILLR-DEREAVSTCYRSS 715

BLAST of Carg23927 vs. Swiss-Prot
Match: sp|A3BF39|HMA2_ORYSJ (Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA2 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 1.0e-233
Identity = 408/684 (59.65%), Postives = 538/684 (78.65%), Query Frame = 0

Query: 20  ERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSLLVSQLQIVKALN 79
           ++S+FDVLGICC SEVPL+E +L+PLEG+++++VIVP+RT+IVVHD   +SQ QIVKALN
Sbjct: 9   QKSYFDVLGICCPSEVPLVEKLLQPLEGVQKVTVIVPSRTVIVVHDVDAISQSQIVKALN 68

Query: 80  EARLEANVQLKGKGISK--KKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFP 139
           +ARLEA+V+  G G  K   KWPSP+ +  GLLL  S  ++ +HPL+W A+ A AAG+ P
Sbjct: 69  QARLEASVRAYGNGSEKITNKWPSPYVLLCGLLLVVSLFEHFWHPLKWFALVAAAAGLPP 128

Query: 140 ILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLESRASHKANAA 199
           I+L++I+A+R L +DVNIL +IAV G +++ DY E G IVFLF+ AEWLE+RASHKA A 
Sbjct: 129 IVLRSIAAIRRLTLDVNILMLIAVAGAIALKDYSEAGFIVFLFTTAEWLETRASHKATAG 188

Query: 200 MWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSFDG 259
           M +LMS+APQKA +AETGEVV  ++VK+ +V+AVK GEVIPIDG+VVDG+ +VDE +  G
Sbjct: 189 MSALMSMAPQKAILAETGEVVAARDVKVNTVIAVKAGEVIPIDGVVVDGRSEVDESTLTG 248

Query: 260 ETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDE 319
           E+FPV K  DS VWAGT+N++GYI+V+TT +A++  VAKMA  VEEAQN++S TQR ID 
Sbjct: 249 ESFPVSKQPDSQVWAGTLNIDGYIAVRTTAMADNSAVAKMARLVEEAQNSRSSTQRLIDT 308

Query: 320 CAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 379
           CAKYYTPAVVV++  VAAIPA  + HNL HWF LALV+LVSACPCAL+LSTP+A FCAL 
Sbjct: 309 CAKYYTPAVVVMAGSVAAIPAIAKAHNLKHWFQLALVLLVSACPCALVLSTPIATFCALL 368

Query: 380 KAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDISFHTLLHWVSS 439
           +AA  G+LIKG D LE LA IKV AFDKTGTITRGEF V  FQ + + +S   LL+WVSS
Sbjct: 369 RAARTGLLIKGGDVLESLASIKVAAFDKTGTITRGEFSVEEFQPVGERVSLQQLLYWVSS 428

Query: 440 IESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIYIGSTKIAARA 499
           +ES+SSHPMA+ LV++A+  S++ K E V EF+ +PGEG+ G+IDG  IYIG+ +I +RA
Sbjct: 429 VESRSSHPMASVLVDYAQSKSVEPKSENVSEFQIYPGEGIYGEIDGAGIYIGNKRILSRA 488

Query: 500 GCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKSLGIKTAMLTG 559
            C +   +++   +G T+GYV C    +G F LSD+CR+G+ E ++EL+SLGIK+ MLTG
Sbjct: 489 SCETVPDMKD--MKGVTIGYVACNNELIGVFTLSDACRTGSAEAIKELRSLGIKSVMLTG 548

Query: 560 DCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGLNDTPALATAD 619
           D SAAA +AQ QL N +  +H+ELLP++K  ++ E K   G T MVGDG+ND PALA AD
Sbjct: 549 DSSAAATYAQNQLGNILAEVHAELLPEDKVRIVGELKEKDGPTLMVGDGMNDAPALAKAD 608

Query: 620 IGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILSIATKIAILGL 679
           +G+SMG+SGS +A +T +V LMSNDI +IP+A++LAR++H  ++ N+I S+ TK+AI+GL
Sbjct: 609 VGVSMGVSGSAVAMETSHVALMSNDIRRIPKAVRLARRTHRTIIVNIIFSVITKLAIVGL 668

Query: 680 AIAGHPLVWAAVLADVGTCLLVIL 702
           A AGHPL+WAAVLADVGTCLLVI+
Sbjct: 669 AFAGHPLIWAAVLADVGTCLLVIM 690

BLAST of Carg23927 vs. Swiss-Prot
Match: sp|Q8H384|HMA3_ORYSJ (Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 1.2e-205
Identity = 374/687 (54.44%), Postives = 498/687 (72.49%), Query Frame = 0

Query: 23  HFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSLLVSQLQIVKALNEAR 82
           + DVLG+CCS+EV L+E +L PL+G++ +SV+V +RT++V HD     +  IVKALN+A 
Sbjct: 45  YLDVLGVCCSAEVALVERLLAPLDGVRVVSVVVASRTVVVEHDPAAAPESAIVKALNKAG 104

Query: 83  LEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFPILLKA 142
           LEA+V+  G      +WPSP+ +ASG+LLTASF ++++ PL+ LAVAAV AG  P++ + 
Sbjct: 105 LEASVRAYGSSGVVSRWPSPYIVASGVLLTASFFEWLFPPLQCLAVAAVVAGAPPMVRRG 164

Query: 143 ISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLESRASHKANAAMWSLM 202
            +A   L +D+N+L +IAV G + + DY E G+IVFLF+ AEWLE+ A  KA+A M SLM
Sbjct: 165 FAAASRLSLDINVLMLIAVAGALCLGDYTEAGAIVFLFTTAEWLETLACTKASAGMSSLM 224

Query: 203 SLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSFDGETFPV 262
            + P KA IA TGEVV V++V++  V+AV+ GE++P+DG+VVDG+ +VDE+S  GE+FPV
Sbjct: 225 GMLPVKAVIATTGEVVSVRDVRVGDVVAVRAGEIVPVDGVVVDGQSEVDERSLTGESFPV 284

Query: 263 PKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDECAKYY 322
           PK   S VWAGT+N +GYI+V+TT +AE+  VAKM   VE AQN++SKTQR ID CAKYY
Sbjct: 285 PKQPHSEVWAGTMNFDGYIAVRTTALAENSTVAKMERLVEAAQNSRSKTQRLIDSCAKYY 344

Query: 323 TPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALTKAAMA 382
           TPAVVV++A VA IPA      L  W+ LALV+LVSACPCAL+LSTPVA+FCA+ +AA  
Sbjct: 345 TPAVVVVAAGVALIPALLGADGLEQWWKLALVMLVSACPCALVLSTPVASFCAMLRAARM 404

Query: 383 GVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRD-DISFHTLLHWVSSIESK 442
           G+ IKG D LE L +I+ VAFDKTGTITRGEF +  F  + D  +    LL+W++SIESK
Sbjct: 405 GIFIKGGDVLESLGEIRAVAFDKTGTITRGEFSIDSFHLVGDHKVEMDHLLYWIASIESK 464

Query: 443 SSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIYIGSTKIAARAGCSS 502
           SSHPMAAALV +A+  SI   PE V +F  +PGEG+ G+I GK IYIG+ +  ARA    
Sbjct: 465 SSHPMAAALVEYAQSKSIQPNPENVGDFRIYPGEGIYGEIHGKHIYIGNRRTLARASSPQ 524

Query: 503 AMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKSLGIKTAMLTGDCSA 562
           + +   EM +G ++GYV C+    G F LSD CR+GA E + EL SLGIK+ MLTGD SA
Sbjct: 525 STQEMGEMIKGVSIGYVICDGELAGVFSLSDDCRTGAAEAIRELGSLGIKSVMLTGDSSA 584

Query: 563 AAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGLNDTPALATADIGIS 622
           AA HAQ QL   ++ +HSELLP++K  ++   K  +G T MVGDG+ND  ALA AD+G+S
Sbjct: 585 AATHAQGQLGGVMEELHSELLPEDKVRLVSGLKARFGPTMMVGDGMNDAAALAAADVGVS 644

Query: 623 MGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILSIATKIAILGLAIAG 682
           MGISGS  A +T +  LMS+D+ ++P+A++L R +   +  NV  S+A K A+L LA A 
Sbjct: 645 MGISGSAAAMETSHATLMSSDVLRVPEAVRLGRCARRTIAVNVAGSVAVKAAVLALAAAW 704

Query: 683 HPLVWAAVLADVGTCLLVILNSMLLLR 709
            P++WAAVLADVGTCLLV+LNSM LLR
Sbjct: 705 RPVLWAAVLADVGTCLLVVLNSMTLLR 731

BLAST of Carg23927 vs. TrEMBL
Match: tr|A0A1S3BSI1|A0A1S3BSI1_CUCME (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492700 PE=3 SV=1)

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 622/742 (83.83%), Postives = 667/742 (89.89%), Query Frame = 0

Query: 2   VGEVAMEK--NKKVRREN-KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTR 61
           + E A EK  NKKV REN KFERSHFDVLGICCSSE+PLIENILKPLEGIKQI+VIVPTR
Sbjct: 1   MSEEATEKTINKKVTRENKKFERSHFDVLGICCSSEIPLIENILKPLEGIKQITVIVPTR 60

Query: 62  TLIVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKY 121
           TLIVVHDSLL+SQLQIVKALNEARLEAN+QLKGKGI KKKWPSP+AIASGLLLTASFLKY
Sbjct: 61  TLIVVHDSLLISQLQIVKALNEARLEANIQLKGKGIMKKKWPSPYAIASGLLLTASFLKY 120

Query: 122 VYHPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVF 181
           VYHPLRWLAVAAVAAGIFPILLKAISA+RHLRIDVNILAIIAVVGT++M DYME GSIVF
Sbjct: 121 VYHPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAIIAVVGTIAMEDYMEAGSIVF 180

Query: 182 LFSIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIP 241
           LFSIAEWLESRAS KAN AM SLM LAPQKATIAE+GEVV+V++VKLKSVL+VK GEVIP
Sbjct: 181 LFSIAEWLESRASQKANGAMSSLMRLAPQKATIAESGEVVDVRDVKLKSVLSVKAGEVIP 240

Query: 242 IDGIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMA 301
           IDGIVV+G C+VDEK+  GETFPV K KDSLVWAGTINLNGY+SVQTTVVAEDCVVAKMA
Sbjct: 241 IDGIVVEGNCEVDEKTLSGETFPVTKQKDSLVWAGTINLNGYVSVQTTVVAEDCVVAKMA 300

Query: 302 EFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVS 361
           E VEEAQ NKSKTQ FIDECAKYYTPAV++ISAC+AAIPAA RVHNLSHW HLALVVLVS
Sbjct: 301 ELVEEAQKNKSKTQTFIDECAKYYTPAVIIISACLAAIPAALRVHNLSHWLHLALVVLVS 360

Query: 362 ACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTH 421
           ACPCALILSTPVAAFCALTKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVVTH
Sbjct: 361 ACPCALILSTPVAAFCALTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVVTH 420

Query: 422 FQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVR 481
           FQALRDDISF+TLL WVSSIESKSSHPMA ALVN+ KL SID+KPE VEEFENFPGEGVR
Sbjct: 421 FQALRDDISFNTLLQWVSSIESKSSHPMATALVNYGKLHSIDLKPENVEEFENFPGEGVR 480

Query: 482 GKIDGKDIYIGSTKIAARAG-----CSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDS 541
           GKIDG DIYIGS KIAARAG      S     ++E +Q QTLGYVFC    +GSFGL DS
Sbjct: 481 GKIDGNDIYIGSKKIAARAGYDIPVSSELNNFDDETRQEQTLGYVFCGGTIIGSFGLLDS 540

Query: 542 CRSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEF 601
           CRSG KE +EE+KS GIKTAMLTGDC AAAMH Q+QL N  +VIHSELLPKEKAN+IKEF
Sbjct: 541 CRSGVKEAIEEIKSFGIKTAMLTGDCRAAAMHVQEQLGNNFEVIHSELLPKEKANIIKEF 600

Query: 602 KRDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLA 661
           K++ GA AMVGDGLNDTPALATADIG+SMGISGS LAT+TGNVILMSND+ KIP+ IKLA
Sbjct: 601 KKNDGAIAMVGDGLNDTPALATADIGMSMGISGSALATETGNVILMSNDMRKIPKVIKLA 660

Query: 662 RKSHAKVVQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHK 721
           +  H KVVQNVILSI TK AILGLA AGHPL+WAAVLADVGTCLLVILNSMLLLRG+DHK
Sbjct: 661 KTFHTKVVQNVILSIGTKTAILGLAFAGHPLIWAAVLADVGTCLLVILNSMLLLRGTDHK 720

Query: 722 HG-NKCCKSSKLCSTKHGR-CD 734
           HG  KCCKSSK C TKHG+ CD
Sbjct: 721 HGKKKCCKSSKPCLTKHGQLCD 742

BLAST of Carg23927 vs. TrEMBL
Match: tr|A0A1S3BS81|A0A1S3BS81_CUCME (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492700 PE=3 SV=1)

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 621/739 (84.03%), Postives = 666/739 (90.12%), Query Frame = 0

Query: 2   VGEVAMEK--NKKVRREN-KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTR 61
           + E A EK  NKKV REN KFERSHFDVLGICCSSE+PLIENILKPLEGIKQI+VIVPTR
Sbjct: 1   MSEEATEKTINKKVTRENKKFERSHFDVLGICCSSEIPLIENILKPLEGIKQITVIVPTR 60

Query: 62  TLIVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKY 121
           TLIVVHDSLL+SQLQIVKALNEARLEAN+QLKGKGI KKKWPSP+AIASGLLLTASFLKY
Sbjct: 61  TLIVVHDSLLISQLQIVKALNEARLEANIQLKGKGIMKKKWPSPYAIASGLLLTASFLKY 120

Query: 122 VYHPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVF 181
           VYHPLRWLAVAAVAAGIFPILLKAISA+RHLRIDVNILAIIAVVGT++M DYME GSIVF
Sbjct: 121 VYHPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAIIAVVGTIAMEDYMEAGSIVF 180

Query: 182 LFSIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIP 241
           LFSIAEWLESRAS KAN AM SLM LAPQKATIAE+GEVV+V++VKLKSVL+VK GEVIP
Sbjct: 181 LFSIAEWLESRASQKANGAMSSLMRLAPQKATIAESGEVVDVRDVKLKSVLSVKAGEVIP 240

Query: 242 IDGIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMA 301
           IDGIVV+G C+VDEK+  GETFPV K KDSLVWAGTINLNGY+SVQTTVVAEDCVVAKMA
Sbjct: 241 IDGIVVEGNCEVDEKTLSGETFPVTKQKDSLVWAGTINLNGYVSVQTTVVAEDCVVAKMA 300

Query: 302 EFVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVS 361
           E VEEAQ NKSKTQ FIDECAKYYTPAV++ISAC+AAIPAA RVHNLSHW HLALVVLVS
Sbjct: 301 ELVEEAQKNKSKTQTFIDECAKYYTPAVIIISACLAAIPAALRVHNLSHWLHLALVVLVS 360

Query: 362 ACPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTH 421
           ACPCALILSTPVAAFCALTKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVVTH
Sbjct: 361 ACPCALILSTPVAAFCALTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVVTH 420

Query: 422 FQALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVR 481
           FQALRDDISF+TLL WVSSIESKSSHPMA ALVN+ KL SID+KPE VEEFENFPGEGVR
Sbjct: 421 FQALRDDISFNTLLQWVSSIESKSSHPMATALVNYGKLHSIDLKPENVEEFENFPGEGVR 480

Query: 482 GKIDGKDIYIGSTKIAARAG--CSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRS 541
           GKIDG DIYIGS KIAARAG         ++E +Q QTLGYVFC    +GSFGL DSCRS
Sbjct: 481 GKIDGNDIYIGSKKIAARAGYDIPELNNFDDETRQEQTLGYVFCGGTIIGSFGLLDSCRS 540

Query: 542 GAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRD 601
           G KE +EE+KS GIKTAMLTGDC AAAMH Q+QL N  +VIHSELLPKEKAN+IKEFK++
Sbjct: 541 GVKEAIEEIKSFGIKTAMLTGDCRAAAMHVQEQLGNNFEVIHSELLPKEKANIIKEFKKN 600

Query: 602 YGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKS 661
            GA AMVGDGLNDTPALATADIG+SMGISGS LAT+TGNVILMSND+ KIP+ IKLA+  
Sbjct: 601 DGAIAMVGDGLNDTPALATADIGMSMGISGSALATETGNVILMSNDMRKIPKVIKLAKTF 660

Query: 662 HAKVVQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHG- 721
           H KVVQNVILSI TK AILGLA AGHPL+WAAVLADVGTCLLVILNSMLLLRG+DHKHG 
Sbjct: 661 HTKVVQNVILSIGTKTAILGLAFAGHPLIWAAVLADVGTCLLVILNSMLLLRGTDHKHGK 720

Query: 722 NKCCKSSKLCSTKHGR-CD 734
            KCCKSSK C TKHG+ CD
Sbjct: 721 KKCCKSSKPCLTKHGQLCD 739

BLAST of Carg23927 vs. TrEMBL
Match: tr|A0A076MEZ2|A0A076MEZ2_CUCSA (Heavy metal ATPase 3 OS=Cucumis sativus OX=3659 GN=HMA3 PE=2 SV=1)

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 612/721 (84.88%), Postives = 654/721 (90.71%), Query Frame = 0

Query: 20  ERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVVHDSLLVSQLQIVKALN 79
           ERSHFDVLGICCSSE+PLIENILKPL+GIKQI+VIVPTRTLIVVHDSLL+SQLQIVKALN
Sbjct: 2   ERSHFDVLGICCSSEIPLIENILKPLQGIKQITVIVPTRTLIVVHDSLLISQLQIVKALN 61

Query: 80  EARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFPIL 139
           EARLEAN+QLKGKGI KKKWPSP+AIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFPIL
Sbjct: 62  EARLEANIQLKGKGIMKKKWPSPYAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFPIL 121

Query: 140 LKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIAEWLESRASHKANAAMW 199
           LKAISA+RHLR+DVNILAIIAVVGT++M+DYME GSIVFLFSIAEWLESRASHKAN AM 
Sbjct: 122 LKAISAIRHLRVDVNILAIIAVVGTIAMDDYMEAGSIVFLFSIAEWLESRASHKANGAMC 181

Query: 200 SLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIVVDGKCDVDEKSFDGET 259
           SLM LAPQKATIAE+GEVV+V++VKLKSVL VK GEVIPIDGIVV+G C+VDEK+  GET
Sbjct: 182 SLMRLAPQKATIAESGEVVDVRDVKLKSVLGVKAGEVIPIDGIVVEGNCEVDEKTLSGET 241

Query: 260 FPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEEAQNNKSKTQRFIDECA 319
           FPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE VEEAQNNKSKTQ FIDECA
Sbjct: 242 FPVTKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNKSKTQTFIDECA 301

Query: 320 KYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALTKA 379
           KYYTPAVV+ISAC+AAIPAA RVHNL HW HLALVVLVSACPCALILSTPVAAFCALTKA
Sbjct: 302 KYYTPAVVIISACLAAIPAALRVHNLRHWLHLALVVLVSACPCALILSTPVAAFCALTKA 361

Query: 380 AMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALRDDISFHTLLHWVSSIE 439
           AMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVVTHFQALRDDI+FHTLL WVSSIE
Sbjct: 362 AMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVVTHFQALRDDINFHTLLQWVSSIE 421

Query: 440 SKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDGKDIYIGSTKIAARAG- 499
           SKSSHPMA ALVN+ KL SID+KPE VEEFENF GEGVRGKIDG DIYIGS KIAARAG 
Sbjct: 422 SKSSHPMATALVNYGKLHSIDLKPENVEEFENFLGEGVRGKIDGNDIYIGSKKIAARAGY 481

Query: 500 ----CSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGMEELKSLGIKTAM 559
                S     ++E +Q QTLGYVFC  M +GSFGL DSCRSG KE +EE+KS GIKTAM
Sbjct: 482 DIPVSSKLNNFDDETRQEQTLGYVFCGGMMIGSFGLLDSCRSGVKEAIEEIKSFGIKTAM 541

Query: 560 LTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMVGDGLNDTPALA 619
           LTGDC AAAMH Q+QL N +DVIHSELLPKEKAN+IKEFK + GA AMVGDGLNDTPALA
Sbjct: 542 LTGDCRAAAMHVQEQLGNTLDVIHSELLPKEKANIIKEFKNNDGAIAMVGDGLNDTPALA 601

Query: 620 TADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQNVILSIATKIAI 679
           TADIG+SMGISGS LAT+TGNVILMSND+ KIP+AIKLA+  H KVVQNVILSI TK AI
Sbjct: 602 TADIGMSMGISGSALATETGNVILMSNDMRKIPKAIKLAKTFHTKVVQNVILSIGTKTAI 661

Query: 680 LGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHG-NKCCKSSKLCSTKHGR-C 734
           LGLA AGHPL+WAAVLADVGTCLLVILNSMLLLRG DHKHG  KCCKSSK C TKHG+ C
Sbjct: 662 LGLAFAGHPLIWAAVLADVGTCLLVILNSMLLLRGMDHKHGKKKCCKSSKPCLTKHGQLC 721

BLAST of Carg23927 vs. TrEMBL
Match: tr|A0A0A0LJB5|A0A0A0LJB5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060450 PE=3 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 593/704 (84.23%), Postives = 636/704 (90.34%), Query Frame = 0

Query: 2   VGEVAMEK-NKKVRREN-KFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRT 61
           + E A EK NKKV +EN K ERSHFDVLGICCSSE+PLIENILKPL+GIKQI+VIVPTRT
Sbjct: 1   MSEEATEKMNKKVIKENKKMERSHFDVLGICCSSEIPLIENILKPLQGIKQITVIVPTRT 60

Query: 62  LIVVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYV 121
           LIVVHDSLL+SQLQIVKALNEARLEAN+QLKGKGI KKKWPSP+AIASGLLLTASFLKYV
Sbjct: 61  LIVVHDSLLISQLQIVKALNEARLEANIQLKGKGIMKKKWPSPYAIASGLLLTASFLKYV 120

Query: 122 YHPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFL 181
           YHPLRWLAVAAVAAGIFPILLKAISA+RHLR+DVNILAIIAVVGT++M+DYME GSIVFL
Sbjct: 121 YHPLRWLAVAAVAAGIFPILLKAISAIRHLRVDVNILAIIAVVGTIAMDDYMEAGSIVFL 180

Query: 182 FSIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPI 241
           FSIAEWLESRASHKAN AM SLM LAPQKATIAE+GEVV+V++VKLKSVL VK GEVIPI
Sbjct: 181 FSIAEWLESRASHKANGAMCSLMRLAPQKATIAESGEVVDVRDVKLKSVLGVKAGEVIPI 240

Query: 242 DGIVVDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE 301
           DGIVV+G C+VDEK+  GETFPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE
Sbjct: 241 DGIVVEGNCEVDEKTLSGETFPVTKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE 300

Query: 302 FVEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSA 361
            VEEAQNNKSKTQ FIDECAKYYTPAVV+ISAC+AAIPAA RVHNL HW HLALVVLVSA
Sbjct: 301 LVEEAQNNKSKTQTFIDECAKYYTPAVVIISACLAAIPAALRVHNLRHWLHLALVVLVSA 360

Query: 362 CPCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHF 421
           CPCALILSTPVAAFCALTKAAMAGVLIKG +HLEVLAKIKV+AFDKTGTITRGEFVVTHF
Sbjct: 361 CPCALILSTPVAAFCALTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVVTHF 420

Query: 422 QALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRG 481
           QALRDDI+FHTLL WVSSIESKSSHPMA ALVN+ KL SID+KPE VEEFENF GEGVRG
Sbjct: 421 QALRDDINFHTLLQWVSSIESKSSHPMATALVNYGKLHSIDLKPENVEEFENFLGEGVRG 480

Query: 482 KIDGKDIYIGSTKIAARAG-----CSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSC 541
           KIDG DIYIGS KIAARAG      S     ++E +Q QTLGYVFC  M +GSFGL DSC
Sbjct: 481 KIDGNDIYIGSKKIAARAGYDIPVSSKLNNFDDETRQEQTLGYVFCGGMMIGSFGLLDSC 540

Query: 542 RSGAKEGMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFK 601
           RSG KE +EE+KS GIKTAMLTGDC AAAMH Q+QL N +DVIHSELLPKEKAN+IKEFK
Sbjct: 541 RSGVKEAIEEIKSFGIKTAMLTGDCRAAAMHVQEQLGNTLDVIHSELLPKEKANIIKEFK 600

Query: 602 RDYGATAMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLAR 661
            + GA AMVGDGLNDTPALATADIG+SMGISGS LAT+TGNVILMSND+ KIP+AIKLA+
Sbjct: 601 NNDGAIAMVGDGLNDTPALATADIGMSMGISGSALATETGNVILMSNDMRKIPKAIKLAK 660

Query: 662 KSHAKVVQNVILSIATKIAILGLAIAGHPLVWAAVLADVGTCLL 699
             H KVVQNVILSI TK AILGLA AGHPL+WAAVLADVGTCLL
Sbjct: 661 TFHTKVVQNVILSIGTKTAILGLAFAGHPLIWAAVLADVGTCLL 704

BLAST of Carg23927 vs. TrEMBL
Match: tr|A0A2I4HLD6|A0A2I4HLD6_9ROSI (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 OS=Juglans regia OX=51240 GN=LOC109019139 PE=3 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.0e-283
Identity = 508/735 (69.12%), Postives = 619/735 (84.22%), Query Frame = 0

Query: 4   EVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIVV 63
           E  M+  +K + + KF++S+FDVLG+CCSSEVPLI NILKPL+G+K++SVIVP+RT++VV
Sbjct: 8   EETMDSQEKSKTQKKFQKSYFDVLGLCCSSEVPLIVNILKPLDGVKEVSVIVPSRTVVVV 67

Query: 64  HDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHPL 123
           HD+LL+SQ+QIVKALN+ARLEANV++ GK   KKKWPSPFA+A G LL  SFLKY Y PL
Sbjct: 68  HDNLLISQIQIVKALNQARLEANVKIYGKENYKKKWPSPFAVACGALLLLSFLKYAYQPL 127

Query: 124 RWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEGGSIVFLFSIA 183
           +WLAV AVA GIFPI LK + A+R++R+DVNIL ++AV+GT+++NDY+E G+IVFLF+IA
Sbjct: 128 QWLAVGAVAVGIFPITLKGLVAIRNIRLDVNILVLVAVMGTLALNDYLEAGTIVFLFTIA 187

Query: 184 EWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVKLKSVLAVKGGEVIPIDGIV 243
           EWLESRAS+KANA M SLMS+APQKA IAETGE V+  EVKL +VLAVK GEVIPIDGIV
Sbjct: 188 EWLESRASYKANAVMSSLMSMAPQKAVIAETGEEVDADEVKLSTVLAVKAGEVIPIDGIV 247

Query: 244 VDGKCDVDEKSFDGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFVEE 303
           V+G+C+VDEK+  GE+FPV K K+S+VWAGTINL+GYISV+TT +AE+CVVAKMA+ VE+
Sbjct: 248 VEGQCEVDEKTLTGESFPVAKQKESIVWAGTINLDGYISVKTTALAEECVVAKMAKLVED 307

Query: 304 AQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPCA 363
           AQN+KS TQR ID+C K+YTPA+++IS   A +PAA +V N +HWFHLALVVLVSACPC+
Sbjct: 308 AQNSKSSTQRIIDKCTKFYTPAILIISIGFAVVPAALKVKNRNHWFHLALVVLVSACPCS 367

Query: 364 LILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTHFQALR 423
           LILSTPVA FCALTKAA +G+LIKG D+LE LAKIKVVAFDKTGTITRGEFVVT FQ+LR
Sbjct: 368 LILSTPVATFCALTKAATSGLLIKGGDYLETLAKIKVVAFDKTGTITRGEFVVTDFQSLR 427

Query: 424 DDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKIDG 483
           DDIS +TLL+WVSSIESKSSHPMAAALV+HA+ LS++ KPE V EF+NFPGEG+ GKIDG
Sbjct: 428 DDISLNTLLYWVSSIESKSSHPMAAALVDHARSLSVEPKPENVMEFQNFPGEGIHGKIDG 487

Query: 484 KDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKEGME 543
            DIYIG+ KIA RAGC +   LE  MK+G+T+GY++     VG F LSD+CRSG  E + 
Sbjct: 488 DDIYIGNRKIALRAGCETVPALEGNMKEGKTIGYIYSGTSPVGIFCLSDACRSGVAEAIR 547

Query: 544 ELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGATAMV 603
           E+K LGIKTAMLTGD  AAAMH Q+QL++ ++V+H+ELLP++KA +IKEFK + G TAMV
Sbjct: 548 EMKLLGIKTAMLTGDRHAAAMHVQEQLDHRLEVVHAELLPEDKARIIKEFKEE-GPTAMV 607

Query: 604 GDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVVQN 663
           GDG+ND PALATADIGISMGISGS LAT+TG VILMSNDI KIP+AI+LARK+  KV+ N
Sbjct: 608 GDGVNDAPALATADIGISMGISGSTLATETGQVILMSNDIRKIPEAIQLARKAQRKVILN 667

Query: 664 VILSIATKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDH--KHGNKCCKS 723
           +ILS  TK AIL LA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+    +  +KC ++
Sbjct: 668 IILSFTTKSAILALAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTQKPVRTSSKCHRA 727

Query: 724 SKLCSTKHGRCDESN 737
               + KH  C+ SN
Sbjct: 728 QH--THKHA-CNASN 738

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022929619.10.0e+0098.78cadmium/zinc-transporting ATPase HMA2-like isoform X1 [Cucurbita moschata][more]
XP_022929620.10.0e+0098.78cadmium/zinc-transporting ATPase HMA2-like isoform X2 [Cucurbita moschata][more]
XP_023545589.10.0e+0097.70putative inactive cadmium/zinc-transporting ATPase HMA3 [Cucurbita pepo subsp. p... [more]
XP_022929621.10.0e+0097.84putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 [Cucurbita mo... [more]
XP_022929622.10.0e+0097.84putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 [Cucurbita mo... [more]
Match NameE-valueIdentityDescription
AT4G30110.12.4e-27867.98heavy metal atpase 2[more]
AT2G19110.11.1e-27566.99heavy metal atpase 4[more]
AT4G30120.14.8e-18660.52heavy metal atpase 3[more]
AT4G33520.29.0e-6029.93P-type ATP-ase 1[more]
AT5G44790.12.2e-5329.70copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPas... [more]
Match NameE-valueIdentityDescription
sp|Q9SZW4|HMA2_ARATH4.3e-27767.98Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE... [more]
sp|O64474|HMA4_ARATH2.0e-27466.99Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 G... [more]
sp|P0CW78|HMA3B_ARATH1.4e-24661.65Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE... [more]
sp|A3BF39|HMA2_ORYSJ1.0e-23359.65Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
sp|Q8H384|HMA3_ORYSJ1.2e-20554.44Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BSI1|A0A1S3BSI1_CUCME0.0e+0083.83putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucumis me... [more]
tr|A0A1S3BS81|A0A1S3BS81_CUCME0.0e+0084.03putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 OS=Cucumis me... [more]
tr|A0A076MEZ2|A0A076MEZ2_CUCSA0.0e+0084.88Heavy metal ATPase 3 OS=Cucumis sativus OX=3659 GN=HMA3 PE=2 SV=1[more]
tr|A0A0A0LJB5|A0A0A0LJB5_CUCSA0.0e+0084.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G060450 PE=3 SV=1[more]
tr|A0A2I4HLD6|A0A2I4HLD6_9ROSI1.0e-28369.12putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 OS=Juglans re... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0030001metal ion transport
GO:0006812cation transport
Vocabulary: Molecular Function
TermDefinition
GO:0046872metal ion binding
GO:0000166nucleotide binding
GO:0019829cation-transporting ATPase activity
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: INTERPRO
TermDefinition
IPR036412HAD-like_sf
IPR023298ATPase_P-typ_TM_dom_sf
IPR036163HMA_dom_sf
IPR008250ATPase_P-typ_transduc_dom_A_sf
IPR006121HMA_dom
IPR018303ATPase_P-typ_P_site
IPR023299ATPase_P-typ_cyto_dom_N
IPR027256P-typ_ATPase_IB
IPR023214HAD_sf
IPR001757P_typ_ATPase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030001 metal ion transport
biological_process GO:0006812 cation transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0019829 cation-transporting ATPase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23927-RACarg23927-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00119CATATPASEcoord: 548..558
score: 55.63
coord: 251..265
score: 37.7
coord: 603..622
score: 63.66
coord: 627..639
score: 21.16
coord: 402..416
score: 48.57
NoneNo IPR availableGENE3DG3DSA:2.70.150.20coord: 181..305
e-value: 1.0E-24
score: 88.7
NoneNo IPR availablePFAMPF00122E1-E2_ATPasecoord: 204..380
e-value: 4.0E-37
score: 127.4
NoneNo IPR availableGENE3DG3DSA:3.30.70.100coord: 16..89
e-value: 5.3E-12
score: 47.9
NoneNo IPR availablePFAMPF00702Hydrolasecoord: 398..617
e-value: 3.1E-33
score: 115.7
NoneNo IPR availableSFLDSFLDF00027p-type_atpasecoord: 384..655
e-value: 4.204E-45
score: 149.0
NoneNo IPR availableSFLDSFLDG00002C1.7:_P-type_atpase_likecoord: 384..655
e-value: 4.204E-45
score: 149.0
NoneNo IPR availablePANTHERPTHR43338FAMILY NOT NAMEDcoord: 11..711
NoneNo IPR availableCDDcd02079P-type_ATPase_HMcoord: 103..705
e-value: 0.0
score: 647.735
IPR001757P-type ATPaseTIGRFAMTIGR01494TIGR01494coord: 176..680
e-value: 2.7E-70
score: 235.1
IPR023214HAD superfamilyGENE3DG3DSA:3.40.50.1000coord: 534..655
e-value: 2.5E-75
score: 255.7
coord: 383..412
e-value: 2.5E-75
score: 255.7
IPR027256P-type ATPase, subfamily IBTIGRFAMTIGR01525TIGR01525coord: 153..706
e-value: 2.4E-167
score: 556.1
IPR023299P-type ATPase, cytoplasmic domain NGENE3DG3DSA:3.40.1110.10coord: 413..533
e-value: 2.5E-75
score: 255.7
IPR018303P-type ATPase, phosphorylation sitePROSITEPS00154ATPASE_E1_E2coord: 404..410
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 21..87
score: 14.243
IPR008250P-type ATPase, A domain superfamilySUPERFAMILYSSF81653Calcium ATPase, transduction domain Acoord: 216..301
IPR036163Heavy metal-associated domain superfamilySUPERFAMILYSSF55008HMA, heavy metal-associated domaincoord: 16..86
IPR023298P-type ATPase, transmembrane domain superfamilySUPERFAMILYSSF81665Calcium ATPase, transmembrane domain Mcoord: 730..774
coord: 302..396
coord: 154..207
IPR036412HAD-like superfamilySUPERFAMILYSSF56784HAD-likecoord: 400..420
coord: 462..704