Carg23752 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23752
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	chaperone protein DnaJ
Location	Cucurbita_argyrosperma_scaffold_213 : 373336 .. 374435 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCGATTCCGGTTTCGATTTCAAATCCCATTTGGCCTCTGAAATCTTCTCCTTCTCCGCTCGTACTGTCTCTTGCGCTCATCGCCATTTTAGCCGCCCTTTCTTCGATTGGTATCGCCTTCTTGGGGTATGGTTCCCCTGTTCTTCTGATTTCATGGATTTTTGTTTGTTTCTTTGTTCTTTAATCGATTTTGATAGGTCGAAGAAGATGCTGGAATCGATTCCATTCGTAGGAGATATCTTAAGCTTGGTGGGATTTCCCTTTTCTTTCTCTTTCCATTGCGATTCTGTTTAGTTTTTGGATTGTTTCGTTTTTGTTCATGAGAATGTGCTTTGGATTTGCAGCTCTCCAACTTCATCCAGACAAAAACCCTCACCCCAAAGCGGAATTTGCCTTCAAGCTTGTCTCTCAGGTTCGATTCGATTCATTTTCTGAAATGTTCTTTGTGATTTTGCCTTTTTTTTTTTTTTTTTTATCTTTTGTGATGGATTTTGGATCACTGTTCGTTTGTTTGTTTTGCATTTAATTTCTTTAATCATGAACTACTCGTGATCTTGATGAACTTTAATGCAATATCAATTTAGAGGTTGAGGAATTTGAATGGGTATCTGTAGGGATATGCTTGTTTGAGTGATAATGTGAAGAGAACGGCATTTGATTTGGATAGAATGAAGAATTTCTGCCCTGATTGCAACACAATTCCTTATACAAACTTGACTGCCTTGAATGTATTGGGAAGCTTTAACAAGAACAACAAACATGGAGTCGTCAAGAACATTAAGGAGAGATTGAGAGAAGAATCAAATGTGATTGAGAATTGTTTGCTGAAGAACACCAGCAGATTCCATCACATAAACCACAAAGAATCCCCAATCTTCAACCCTTCTGATTATGTAAGTCAAGGATACCCTCATTTTCGAACCCGAATCCACCCGAAACCGCAGAATTTTCGGTGTTTTCGGTCTGGGAATTCGCTGAAGTATGAACAAGGTAGGGGGCAGTTTGAGTACCCATTGTTTGAGGTTAGATCAGAAAGCTTTCATTTACAGAAACAATCAGCTTTTGTTTATTCAAATGATAGCAAATCCCATTGA

mRNA sequence

ATGGCTTCCGATTCCGGTTTCGATTTCAAATCCCATTTGGCCTCTGAAATCTTCTCCTTCTCCGCTCGTACTGTCTCTTGCGCTCATCGCCATTTTAGCCGCCCTTTCTTCGATTGGTATCGCCTTCTTGGGGTCGAAGAAGATGCTGGAATCGATTCCATTCGTAGGAGATATCTTAAGCTTGCTCTCCAACTTCATCCAGACAAAAACCCTCACCCCAAAGCGGAATTTGCCTTCAAGCTTGTCTCTCAGGGATATGCTTGTTTGAGTGATAATGTGAAGAGAACGGCATTTGATTTGGATAGAATGAAGAATTTCTGCCCTGATTGCAACACAATTCCTTATACAAACTTGACTGCCTTGAATGTATTGGGAAGCTTTAACAAGAACAACAAACATGGAGTCGTCAAGAACATTAAGGAGAGATTGAGAGAAGAATCAAATGTGATTGAGAATTGTTTGCTGAAGAACACCAGCAGATTCCATCACATAAACCACAAAGAATCCCCAATCTTCAACCCTTCTGATTATGTAAGTCAAGGATACCCTCATTTTCGAACCCGAATCCACCCGAAACCGCAGAATTTTCGGTGTTTTCGGTCTGGGAATTCGCTGAAGTATGAACAAGGTAGGGGGCAGTTTGAGTACCCATTGTTTGAGGTTAGATCAGAAAGCTTTCATTTACAGAAACAATCAGCTTTTGTTTATTCAAATGATAGCAAATCCCATTGA

Coding sequence (CDS)

ATGGCTTCCGATTCCGGTTTCGATTTCAAATCCCATTTGGCCTCTGAAATCTTCTCCTTCTCCGCTCGTACTGTCTCTTGCGCTCATCGCCATTTTAGCCGCCCTTTCTTCGATTGGTATCGCCTTCTTGGGGTCGAAGAAGATGCTGGAATCGATTCCATTCGTAGGAGATATCTTAAGCTTGCTCTCCAACTTCATCCAGACAAAAACCCTCACCCCAAAGCGGAATTTGCCTTCAAGCTTGTCTCTCAGGGATATGCTTGTTTGAGTGATAATGTGAAGAGAACGGCATTTGATTTGGATAGAATGAAGAATTTCTGCCCTGATTGCAACACAATTCCTTATACAAACTTGACTGCCTTGAATGTATTGGGAAGCTTTAACAAGAACAACAAACATGGAGTCGTCAAGAACATTAAGGAGAGATTGAGAGAAGAATCAAATGTGATTGAGAATTGTTTGCTGAAGAACACCAGCAGATTCCATCACATAAACCACAAAGAATCCCCAATCTTCAACCCTTCTGATTATGTAAGTCAAGGATACCCTCATTTTCGAACCCGAATCCACCCGAAACCGCAGAATTTTCGGTGTTTTCGGTCTGGGAATTCGCTGAAGTATGAACAAGGTAGGGGGCAGTTTGAGTACCCATTGTTTGAGGTTAGATCAGAAAGCTTTCATTTACAGAAACAATCAGCTTTTGTTTATTCAAATGATAGCAAATCCCATTGA

Protein sequence

MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTALNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQGYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAFVYSNDSKSH

Homology

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BLAST of Carg23752 vs. NCBI nr
Match: XP_022921687.1 (dnaJ homolog subfamily C member 12-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 506.1 bits (1302), Expect = 6.5e-140
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60
           MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK
Sbjct: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60

Query: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA 120
           LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA
Sbjct: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA 120

Query: 121 LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180
           LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ
Sbjct: 121 LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180

Query: 181 GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAFVYSNDS 240
           GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAFVYSNDS
Sbjct: 181 GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAFVYSNDS 240

Query: 241 KSH 244
           KSH
Sbjct: 241 KSH 243

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BLAST of Carg23752 vs. NCBI nr
Match: XP_022921601.1 (dnaJ homolog subfamily C member 12-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 488.8 bits (1257), Expect = 1.1e-134
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60
           MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK
Sbjct: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60

Query: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA 120
           LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA
Sbjct: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA 120

Query: 121 LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180
           LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ
Sbjct: 121 LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180

Query: 181 GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAF 235
           GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAF
Sbjct: 181 GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAF 234

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BLAST of Carg23752 vs. NCBI nr
Match: XP_023539077.1 (dnaJ homolog subfamily C member 12-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 488.8 bits (1257), Expect = 1.1e-134
Identity = 235/243 (96.71%), Postives = 237/243 (97.53%), Query Frame = 0


Query: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60
           MASDSG DFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK
Sbjct: 1   MASDSGCDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60

Query: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA 120
           LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKR AFDLDRMK FCPDCNTIPYTNL A
Sbjct: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRRAFDLDRMKKFCPDCNTIPYTNLNA 120

Query: 121 LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180
           LNVLGSFNKNNKHG VKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ
Sbjct: 121 LNVLGSFNKNNKHGGVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180

Query: 181 GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAFVYSNDS 240
           GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRG+FEYPLFEVRSESFHLQ+QSAFVYSNDS
Sbjct: 181 GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGKFEYPLFEVRSESFHLQEQSAFVYSNDS 240

Query: 241 KSH 244
           K H
Sbjct: 241 KPH 243

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BLAST of Carg23752 vs. NCBI nr
Match: XP_022972282.1 (dnaJ homolog subfamily C member 12-like [Cucurbita maxima])

HSP 1 Score: 456.4 bits (1173), Expect = 5.9e-125
Identity = 219/233 (93.99%), Postives = 222/233 (95.28%), Query Frame = 0


Query: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60
           MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK
Sbjct: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60

Query: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTA 120
           LALQLHPDKNPHPKAEF+FKLVSQGYACLSDNVKR AFDLDRMK FCPDCNTIPY NL A
Sbjct: 61  LALQLHPDKNPHPKAEFSFKLVSQGYACLSDNVKRRAFDLDRMKKFCPDCNTIPYRNLNA 120

Query: 121 LNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180
           LNVLGSFNKNNKHG  KNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ
Sbjct: 121 LNVLGSFNKNNKHGGFKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQ 180

Query: 181 GYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSA 234
           GYPHFRTRIHPKPQ F C RS NSLKYE+G G+FEYPLFEVRSESFHLQKQSA
Sbjct: 181 GYPHFRTRIHPKPQKFPCLRSRNSLKYEEGMGKFEYPLFEVRSESFHLQKQSA 233

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BLAST of Carg23752 vs. NCBI nr
Match: XP_023539078.1 (uncharacterized protein LOC111799831 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368.2 bits (944), Expect = 2.1e-98
Identity = 175/182 (96.15%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 62  ALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTAL 121
           ALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKR AFDLDRMK FCPDCNTIPYTNL AL
Sbjct: 35  ALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRRAFDLDRMKKFCPDCNTIPYTNLNAL 94

Query: 122 NVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQG 181
           NVLGSFNKNNKHG VKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQG
Sbjct: 95  NVLGSFNKNNKHGGVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSDYVSQG 154

Query: 182 YPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEVRSESFHLQKQSAFVYSNDSK 241
           YPHFRTRIHPKPQNFRCFRSGNSLKYEQGRG+FEYPLFEVRSESFHLQ+QSAFVYSNDSK
Sbjct: 155 YPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGKFEYPLFEVRSESFHLQEQSAFVYSNDSK 214

Query: 242 SH 244
            H
Sbjct: 215 PH 216

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BLAST of Carg23752 vs. TAIR10
Match: AT3G06778.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 155.6 bits (392), Expect = 3.9e-38
Identity = 85/204 (41.67%), Postives = 118/204 (57.84%), Query Frame = 0


Query: 6   GFDFKSHLASEIFSFSARTVSCAHRH---FSRPFFDWYRLLGVEEDAGIDSIRRRYLKLA 65
           G D K+ L S+I + S   +SC H +       F DWY +LG++EDA +  IR+RY KLA
Sbjct: 7   GPDPKAALVSDILALSRSPISCIHINRISSGSCFIDWYLILGIQEDAEVKVIRKRYHKLA 66

Query: 66  LQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTALN 125
           L++HPDKN HPKA+ AFKL+ + Y CLSD  KR +F++DR  N C  C+ + + +    N
Sbjct: 67  LKVHPDKNNHPKADIAFKLIHEAYLCLSDETKRRSFNIDRRNNICLKCSRVSHKSKENRN 126

Query: 126 VLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRF-----------------HHIN 185
                  N     +K+I+++ REE+ VIE CL  N++ F                 + IN
Sbjct: 127 ---DSKPNRFCQTLKDIRDKFREENMVIERCLKTNSAFFMGNRTNETPPVYGIPKQNRIN 186

Query: 186 HKESPIFNPSDYVSQGYPHFRTRI 190
            KESP+FNPSDY   GYPH R R+
Sbjct: 187 -KESPVFNPSDYRLWGYPHVRNRV 206

[Close]

BLAST of Carg23752 vs. TAIR10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977))

HSP 1 Score: 73.2 bits (178), Expect = 2.5e-13
Identity = 31/62 (50.00%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 38  DWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTA 97
           D+Y +LG+E++  +D IR+ Y KL+L++HPDKN  P +E AFK VS+ + CLSD   R  
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 98  FD 100
           FD
Sbjct: 159 FD 160

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BLAST of Carg23752 vs. TAIR10
Match: AT2G35540.1 (DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 70.1 bits (170), Expect = 2.1e-12
Identity = 30/65 (46.15%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 36  FFDWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKR 95
           F +WY++L VE  + I++I+++Y KLAL LHPDKNP+   E  FKL+++ +   SD V+R
Sbjct: 69  FPEWYKVLKVEPFSHINTIKQQYRKLALVLHPDKNPYVGCEEGFKLLNEAFRVFSDKVRR 128

Query: 96  TAFDL 101
           T +D+
Sbjct: 129 TEYDM 133

[Close]

BLAST of Carg23752 vs. TAIR10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977))

HSP 1 Score: 70.1 bits (170), Expect = 2.1e-12
Identity = 28/63 (44.44%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 38  DWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTA 97
           D+Y +LG+E +  +D +R+ Y KL+L++HPDKN  P +E AFK VS+ + CLS++  R  
Sbjct: 113 DYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKK 172

Query: 98  FDL 101
           +D+
Sbjct: 173 YDV 175

[Close]

BLAST of Carg23752 vs. TAIR10
Match: AT5G18750.1 (DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 69.7 bits (169), Expect = 2.8e-12
Identity = 36/82 (43.90%), Postives = 47/82 (57.32%), Query Frame = 0


Query: 25  VSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQ 84
           V CA    S    DWY++L VE+ A  ++I+++Y KLAL LHPDKN  P AE AFK + +
Sbjct: 53  VHCAALEKSGDETDWYKILQVEQTADENTIKKQYKKLALHLHPDKNKLPGAESAFKTIGE 112

Query: 85  GYACLSDNVKRTAFDLDRMKNF 107
               L D  KR   D+ R   F
Sbjct: 113 AQRVLLDKDKRRFHDMRRKPVF 134

[Close]

BLAST of Carg23752 vs. Swiss-Prot
Match: sp|Q9FH28|DNJ49_ARATH (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 4.6e-12
Identity = 31/62 (50.00%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 38  DWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTA 97
           D+Y +LG+E++  +D IR+ Y KL+L++HPDKN  P +E AFK VS+ + CLSD   R  
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 98  FD 100
           FD
Sbjct: 159 FD 160

[Close]

BLAST of Carg23752 vs. Swiss-Prot
Match: sp|Q8TQR1|DNAJ_METAC (Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 6.0e-12
Identity = 31/62 (50.00%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 38  DWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTA 97
           D+Y +LG+ +DA ++ I++ Y KLALQ HPD+N  P AE  FK +S+ YA LSD  KR  
Sbjct: 6   DYYEILGLPKDASVEDIKKTYRKLALQYHPDRNKDPGAEDKFKEISEAYAVLSDTEKRAQ 65

Query: 98  FD 100
           +D
Sbjct: 66  YD 67

[Close]

BLAST of Carg23752 vs. Swiss-Prot
Match: sp|P0CW07|DNAJ_METMA (Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 30/62 (48.39%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 38  DWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTA 97
           D+Y +LG+ +D+ ++ I++ Y KLALQ HPD+N  P AE  FK +S+ YA LSD  KR  
Sbjct: 6   DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 98  FD 100
           +D
Sbjct: 66  YD 67

[Close]

BLAST of Carg23752 vs. Swiss-Prot
Match: sp|P0CW06|DNAJ_METMZ (Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 30/62 (48.39%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 38  DWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTA 97
           D+Y +LG+ +D+ ++ I++ Y KLALQ HPD+N  P AE  FK +S+ YA LSD  KR  
Sbjct: 6   DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 98  FD 100
           +D
Sbjct: 66  YD 67

[Close]

BLAST of Carg23752 vs. Swiss-Prot
Match: sp|Q24133|DNAJ1_DROME (DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3)

HSP 1 Score: 70.1 bits (170), Expect = 3.9e-11
Identity = 31/62 (50.00%), Postives = 42/62 (67.74%), Query Frame = 0


Query: 38  DWYRLLGVEEDAGIDSIRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTA 97
           D+Y++LG+E  A  D I++ Y KLAL+ HPDKN  P+AE  FK +++ Y  LSD  KR  
Sbjct: 4   DFYKILGLERKASDDEIKKAYRKLALKYHPDKNKSPQAEERFKEIAEAYEVLSDKKKRDI 63

Query: 98  FD 100
           FD
Sbjct: 64  FD 65

[Close]

BLAST of Carg23752 vs. TrEMBL
Match: tr|A0A1S3AXH7|A0A1S3AXH7_CUCME (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.9e-95
Identity = 183/266 (68.80%), Postives = 204/266 (76.69%), Query Frame = 0


Query: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLK 60
           M  DS  DFKSHLA EI SFS+ TVSC HRHFS PF DWYRL GV++DA ID IR RYLK
Sbjct: 1   MGPDS--DFKSHLAYEISSFSSLTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLK 60

Query: 61  LALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPY----- 120
           LALQLHPDKN HPKAE AFKLVS+GY CLSDNVKR AFDLDR   FCPDCNTIPY     
Sbjct: 61  LALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTS 120

Query: 121 -TNLTALNVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTS--------------- 180
            TNL ALN+ G F+ N   G ++++KERLREE+NVIENCL  N S               
Sbjct: 121 ATNLNALNIFGGFHNNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPPGCN 180

Query: 181 RFHHINHKESPIFNPSDYVSQGYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLF 240
            FHH N+KE+PIFNPSDYV QGYPHFRTRIHPK ++FRCFRSGN+LKYEQGRG++EYP+F
Sbjct: 181 MFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEYPVF 240

Query: 241 EV---RSESFHLQKQSAFVYSNDSKS 243
           E+   RSESF LQKQSAFVYSN +KS
Sbjct: 241 EISSSRSESFLLQKQSAFVYSNVNKS 264

[Close]

BLAST of Carg23752 vs. TrEMBL
Match: tr|A0A0A0L5B2|A0A0A0L5B2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.5e-94
Identity = 185/260 (71.15%), Postives = 203/260 (78.08%), Query Frame = 0


Query: 8   DFKSHLASEIFSFSARTVSCAHRHFSRPFFDWYRLLGVEEDAGIDSIRRRYLKLALQLHP 67
           DFKSHLASEI SFS+ TVSC HR FS PF DWYRL GV++DA ID IR RYLKLALQLHP
Sbjct: 6   DFKSHLASEISSFSSLTVSCPHRLFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLALQLHP 65

Query: 68  DKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPY------TNLTAL 127
           DKN HPKAE AFKLVS+GY CLSDNVKR AFDLDR   FC DCNTIPY      TNL AL
Sbjct: 66  DKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKDKFCADCNTIPYRNYTSATNLNAL 125

Query: 128 NVLGSFNKNNKHG-VVKNIKERLREESNVIENCLLKNT---------------SRFHHIN 187
           NVLGSF+ NN HG  ++++KERLREE+NVIE CL  N                S FHH N
Sbjct: 126 NVLGSFH-NNIHGRGLRDVKERLREEANVIEYCLRANNSSTTTETSLFNPPGCSMFHHRN 185

Query: 188 HKESPIFNPSDYVSQGYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQFEYPLFEV---R 243
           +KE+PIFNPSDYV QGYPHFRTRIHPKPQ+FRCFRSGN+LKYEQGRG+ EYP+FE+   R
Sbjct: 186 YKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRSGNALKYEQGRGKCEYPVFEISSNR 245

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BLAST of Carg23752 vs. TrEMBL
Match: tr|M5XAD0|M5XAD0_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G129600 PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-58
Identity = 137/273 (50.18%), Postives = 170/273 (62.27%), Query Frame = 0


Query: 1   MASDSGFDFKSHLASEIFSFSARTVSCAHRH-------FSRPFFDWYRLLGVEEDAGIDS 60
           MA DS  DFKS L +EI S S R+++C+HRH        S  F DWYRLL V+E+AG D 
Sbjct: 1   MAHDS--DFKSQLVTEICSISTRSIACSHRHGCSNSPAKSSSFIDWYRLLRVQENAGRDV 60

Query: 61  IRRRYLKLALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTI 120
           IR+RY +LALQLHPDKN HPKAE AFKLVS+ Y+CLSDN KR AFDL+R + FC +C TI
Sbjct: 61  IRKRYHELALQLHPDKNKHPKAEIAFKLVSEAYSCLSDNAKRRAFDLERWRKFCFECGTI 120

Query: 121 PY-TNLTALNVLGSFNK-------NNKHGVVKNIKE---RLREESNVIENCLLKNTSR-- 180
           PY T+ T+ N   S +K       +    VVK +K+   R REE+ VIENCL  N +   
Sbjct: 121 PYTTHKTSSNASASEHKACNPTSSSRSCKVVKGLKDIRNRFREEARVIENCLRANAAAAP 180

Query: 181 ---------------FHHINHKESPIFNPSDYVSQGYPHFRTRIHPKPQNFRCFRSGNS- 238
                          F   + +ESPIFNPSD+  QGYPH RTRIH KPQNF   R+G+  
Sbjct: 181 RRESSLFSPPAAYDVFQSRSSRESPIFNPSDHKVQGYPHLRTRIHSKPQNFWHLRTGHQV 240

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BLAST of Carg23752 vs. TrEMBL
Match: tr|A0A2P6QYN7|A0A2P6QYN7_ROSCH (Putative DnaJ domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0423791 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 6.8e-53
Identity = 133/271 (49.08%), Postives = 164/271 (60.52%), Query Frame = 0


Query: 8   DFKSHLASEIFSFSARTVSCAHRH------FSRPFFDWYRLLGVEEDAGIDSIRRRYLKL 67
           DFKS L +EI S S  ++ C+HRH       S PF DWYRLLGVEE AGID IR+RY +L
Sbjct: 6   DFKSQLVTEICSISKFSIVCSHRHCTSPVRSSSPFVDWYRLLGVEETAGIDVIRKRYHEL 65

Query: 68  ALQLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNL-TA 127
           ALQLHPDKN H KAE AFKLVS+ Y+CLSD+  R AFDL+R   FC +C TIPYT   T 
Sbjct: 66  ALQLHPDKNKHAKAEVAFKLVSEAYSCLSDHENRRAFDLERWSKFCFECGTIPYTTYKTP 125

Query: 128 LNVLGSFNK-------NNKHGVVKNIKE---RLREESNVIENCLLKN------------- 187
            N   S +K       +    V+K +K+   R REE+ VIENCL  N             
Sbjct: 126 SNAKASEHKGWNPTSSSRSCKVIKGLKDMRNRFREEARVIENCLRANNAASRNESSLFSP 185

Query: 188 TSRFHHIN-----HKESPIFNPSDYVSQGYPHFRTRIH--PKPQNFRCFRSGNS-LKYEQ 238
           TS     N      +E+P+FNPSD+V QGYPH RTRI+   K ++F C R+G+  L YEQ
Sbjct: 186 TSYMFQSNSNPRLSRETPVFNPSDHVVQGYPHVRTRIYDKDKSRSFWCLRTGHHILNYEQ 245

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BLAST of Carg23752 vs. TrEMBL
Match: tr|A0A2I4EEZ3|A0A2I4EEZ3_9ROSI (pre-mRNA-splicing factor cwf23-like OS=Juglans regia OX=51240 GN=LOC108988981 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.4e-52
Identity = 121/257 (47.08%), Postives = 157/257 (61.09%), Query Frame = 0


Query: 8   DFKSHLASEIFSFSARTVSCAHRHFSRPF----FDWYRLLGVEEDAGIDSIRRRYLKLAL 67
           D KSHL +EI S S R VSC HRH   PF     DWYR+LGVEE+   D IR+RY KLAL
Sbjct: 9   DLKSHLVTEICSISTRPVSCTHRHGRSPFKSHLIDWYRVLGVEENEKTDVIRKRYHKLAL 68

Query: 68  QLHPDKNPHPKAEFAFKLVSQGYACLSDNVKRTAFDLDRMKNFCPDCNTIPYTNLTAL-- 127
           QLHPDKN HPKAE AFKLVS+ Y CLSD  KR AFDL+R KNFC +C T PY+  ++   
Sbjct: 69  QLHPDKNKHPKAEIAFKLVSEAYTCLSDGAKRRAFDLERWKNFCFECKTNPYSICSSRRN 128

Query: 128 -----NVLGSFNKNNKHGVVKNIKERLREESNVIENCLLKNTSRFHHINHKESPIFNPSD 187
                NV    +++  HG +K+I+ER +EE+ VIENC+  N +     + KESP+F PS+
Sbjct: 129 CNGSENVWNLESQSRSHG-LKDIRERFKEEAKVIENCIRANAT-----SRKESPLFTPSE 188

Query: 188 --------------------YVSQGYPHFRTRIHPKPQNFRCFRSGNSLKYEQGRGQ-FE 233
                               +  QGYPH RTRI  +P  +   ++G+ + YE GR + ++
Sbjct: 189 XXXXXXXXXXXXXXXXXXXXHNVQGYPHLRTRICRQPGKYWYLQTGSRVNYEGGRSRGYD 248

The following BLAST results are available for this feature:

BLAST of Carg23752 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022921687.1	6.5e-140	100.00	dnaJ homolog subfamily C member 12-like isoform X2 [Cucurbita moschata]	[more]
XP_022921601.1	1.1e-134	100.00	dnaJ homolog subfamily C member 12-like isoform X1 [Cucurbita moschata]	[more]
XP_023539077.1	1.1e-134	96.71	dnaJ homolog subfamily C member 12-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022972282.1	5.9e-125	93.99	dnaJ homolog subfamily C member 12-like [Cucurbita maxima]	[more]
XP_023539078.1	2.1e-98	96.15	uncharacterized protein LOC111799831 isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg23752 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G06778.1	3.9e-38	41.67	Chaperone DnaJ-domain superfamily protein	[more]
AT5G49060.1	2.5e-13	50.00	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT2G35540.1	2.1e-12	46.15	DNAJ heat shock N-terminal domain-containing protein	[more]
AT3G57340.1	2.1e-12	44.44	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT5G18750.1	2.8e-12	43.90	DNAJ heat shock N-terminal domain-containing protein	[more]

BLAST of Carg23752 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FH28\|DNJ49_ARATH	4.6e-12	50.00	Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2	[more]
sp\|Q8TQR1\|DNAJ_METAC	6.0e-12	50.00	Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 28...	[more]
sp\|P0CW07\|DNAJ_METMA	1.3e-11	48.39	Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 /...	[more]
sp\|P0CW06\|DNAJ_METMZ	1.3e-11	48.39	Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1	[more]
sp\|Q24133\|DNAJ1_DROME	3.9e-11	50.00	DnaJ protein homolog 1 OS=Drosophila melanogaster OX=7227 GN=DnaJ-1 PE=1 SV=3	[more]

BLAST of Carg23752 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3AXH7\|A0A1S3AXH7_CUCME	1.9e-95	68.80	chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1	[more]
tr\|A0A0A0L5B2\|A0A0A0L5B2_CUCSA	3.5e-94	71.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1	[more]
tr\|M5XAD0\|M5XAD0_PRUPE	1.4e-58	50.18	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G129600 PE=4 SV=1	[more]
tr\|A0A2P6QYN7\|A0A2P6QYN7_ROSCH	6.8e-53	49.08	Putative DnaJ domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_C...	[more]
tr\|A0A2I4EEZ3\|A0A2I4EEZ3_9ROSI	3.4e-52	47.08	pre-mRNA-splicing factor cwf23-like OS=Juglans regia OX=51240 GN=LOC108988981 PE...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR018253	DnaJ_domain_CS
IPR036869	J_dom_sf
IPR001623	DnaJ_domain

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23752-RA	Carg23752-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 58..73 score: 52.91 coord: 40..58 score: 38.04 coord: 74..94 score: 39.59
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 37..94 e-value: 2.5E-18 score: 76.9
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 38..99 e-value: 5.1E-18 score: 64.9
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 38..102 score: 15.854
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 38..91 e-value: 6.53546E-17 score: 71.0373
IPR036869	Chaperone J-domain superfamily	GENE3D	G3DSA:1.10.287.110		coord: 25..110 e-value: 3.3E-20 score: 74.0
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	SSF46565	Chaperone J-domain	coord: 26..102
None	No IPR available	PANTHER	PTHR44137	FAMILY NOT NAMED	coord: 38..105
None	No IPR available	PANTHER	PTHR44137:SF4	SUBFAMILY NOT NAMED	coord: 38..105
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 79..98

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg23752	Cla021766	Watermelon (97103) v1	carwmB0953
Carg23752	Csa3G165080	Cucumber (Chinese Long) v2	carcuB0967
Carg23752	MELO3C006689	Melon (DHL92) v3.5.1	carmeB0934
Carg23752	ClCG05G006300	Watermelon (Charleston Gray)	carwcgB0929
Carg23752	CSPI03G14720	Wild cucumber (PI 183967)	carcpiB0989
Carg23752	Cucsa.252800	Cucumber (Gy14) v1	carcgyB1072
Carg23752	CmaCh06G015440	Cucurbita maxima (Rimu)	carcmaB1307
Carg23752	CmoCh14G016410	Cucurbita moschata (Rifu)	carcmoB1273
Carg23752	CmoCh06G015420	Cucurbita moschata (Rifu)	carcmoB1276
Carg23752	Lsi05G015650	Bottle gourd (USVL1VR-Ls)	carlsiB869
Carg23752	Cp4.1LG08g05060	Cucurbita pepo (Zucchini)	carcpeB1321
Carg23752	Cp4.1LG03g11600	Cucurbita pepo (Zucchini)	carcpeB1320
Carg23752	CsGy3G014850	Cucumber (Gy14) v2	carcgybB0939
Carg23752	MELO3C006689.2	Melon (DHL92) v3.6.1	carmedB0940
Carg23752	CsaV3_3G014930	Cucumber (Chinese Long) v3	carcucB0995
Carg23752	Cla97C05G087880	Watermelon (97103) v2	carwmbB1003
Carg23752	Bhi01G000752	Wax gourd	carwgoB1105

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg23752	Carg19646	Silver-seed gourd	carcarB318

The following block(s) are covering this gene:


Gene	Organism	Block
Carg23752	Cucurbita maxima (Rimu)	carcmaB1304