Carg23536 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23536
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	RNA-binding protein pno1
Location	Cucurbita_argyrosperma_scaffold_376 : 42295 .. 43609 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGGATCGTTAAACCCTAAGAGAGCCGCCTCATTTCTTCGCACTTTCTCCTCTTCCTCTTGGCAGTTAACTTAGCACCTTCCCGCCGTCTCCTCCGTCGTTCTCTGTGCCACCGTCGACGCCGTACCCCTTCTTCACATATTTGTCTACCTTTCTTGGTCTCAGGTATTCTCCCTATTTACCCCATATTTGTCTACCTTTCAAGTTTCATTCCGTTGCAGTGGATGACATCCCAATGGGAACTGATTTTAAATTTTGTTTTGCCGAAGTTTCATTAGTTTGGTTGCTCATTCGTATGATCCGAGGCCGACGCATTCTATTCTTGTGTTACCTAAAATTGAAATTGGATTGTATATTTTAGAACCCGGCACTGTTTTTAGTTCAATCCTACTTGTTTGTTATTAGTTAATAGTTATATTGGGTTATAGTATCTTAGAATCAATGCATCTTTTACATGAGAAAAGGAATTTTTGTTTCTGAAATTCTTCTTGGGAGGATAAGAATGAAGTTTATCTGATTTATTTTCAATTTTTGTTGCTTATCATGTTGATGGGGTTTTGTTTTCATTTGATCTATTACTTCGTCGTTTAACACTAGTTCATTGGTTGTTTACAGTGTGGAAACTCAGAACAATTCTATAATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCATCTCAACCGAAGCCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAAATTTACACCCCAATTTATGAGCAGATGAAAATTGACATCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCGTTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACCTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAAAGGTTTTGA

mRNA sequence

AAAGGATCGTTAAACCCTAAGAGAGCCGCCTCATTTCTTCGCACTTTCTCCTCTTCCTCTTGGCAGTTAACTTAGCACCTTCCCGCCGTCTCCTCCGTCGTTCTCTGTGCCACCGTCGACGCCGTACCCCTTCTTCACATATTTGTCTACCTTTCTTGGTCTCAGTGTGGAAACTCAGAACAATTCTATAATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCATCTCAACCGAAGCCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAAATTTACACCCCAATTTATGAGCAGATGAAAATTGACATCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCGTTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACCTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAAAGGTTTTGA

Coding sequence (CDS)

ATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCATCTCAACCGAAGCCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCTCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAAATTTACACCCCAATTTATGAGCAGATGAAAATTGACATCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCGTTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACCTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAAAGGTTTTGA

Protein sequence

MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF

Homology

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BLAST of Carg23536 vs. NCBI nr
Match: XP_022951370.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >XP_022951376.1 RNA-binding protein pno1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 434.5 bits (1116), Expect = 2.2e-118
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

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BLAST of Carg23536 vs. NCBI nr
Match: XP_023005535.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita maxima] >XP_023005536.1 RNA-binding protein pno1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 433.0 bits (1112), Expect = 6.4e-118
Identity = 222/224 (99.11%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

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BLAST of Carg23536 vs. NCBI nr
Match: XP_023540523.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540524.1 RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432.2 bits (1110), Expect = 1.1e-117
Identity = 221/224 (98.66%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

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BLAST of Carg23536 vs. NCBI nr
Match: XP_022951385.1 (RNA-binding protein pno1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 420.6 bits (1080), Expect = 3.3e-114
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 10  MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 69
           MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT
Sbjct: 1   MEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 60

Query: 70  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE 129
           PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE
Sbjct: 61  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDE 120

Query: 130 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 189
           LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN
Sbjct: 121 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 180

Query: 190 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 215

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BLAST of Carg23536 vs. NCBI nr
Match: XP_008449001.1 (PREDICTED: RNA-binding protein pno1 [Cucumis melo])

HSP 1 Score: 420.2 bits (1079), Expect = 4.3e-114
Identity = 215/224 (95.98%), Postives = 220/224 (98.21%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVE +S+Q K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIY+QMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

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BLAST of Carg23536 vs. TAIR10
Match: AT3G13230.1 (RNA-binding KH domain-containing protein)

HSP 1 Score: 345.9 bits (886), Expect = 1.9e-95
Identity = 174/216 (80.56%), Postives = 197/216 (91.20%), Query Frame = 0


Query: 7   STSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWME 66
           ST MEVE  +    P    LPPKP F PLK HEM+DG+VQFRK++VPP+RYSPLKKAW++
Sbjct: 4   STQMEVETATEGTVP----LPPKPTFKPLKAHEMSDGKVQFRKIAVPPNRYSPLKKAWLD 63

Query: 67  IYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLR 126
           IYTPIY+QMK+DIRMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFD+ DA++LLR
Sbjct: 64  IYTPIYDQMKVDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDIPDAISLLR 123

Query: 127 VDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGS 186
           +DELYVESFEIKDVKTL+GEHLSRAIGRLSGK GKTKFAIEN+TKTRIVIADT+IHILG+
Sbjct: 124 MDELYVESFEIKDVKTLKGEHLSRAIGRLSGKGGKTKFAIENSTKTRIVIADTRIHILGA 183

Query: 187 FANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 223
           F+NIK+AR SLCSLI+GSPAGKVYSKLR+V+ARL E
Sbjct: 184 FSNIKVARSSLCSLIMGSPAGKVYSKLRSVSARLNE 215

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BLAST of Carg23536 vs. Swiss-Prot
Match: sp|Q9VR89|PNO1_DROME (RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 3.9e-66
Identity = 123/187 (65.78%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 37  PHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADT 96
           P +    R + RKVSVPPHRYS LK+ WM+I+TP+ E MK+ IR N+KAR+VEL+   +T
Sbjct: 53  PPKAKRARSELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPET 112

Query: 97  PDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLS 156
           PDI+NLQ+ ADFV AF+ GF+V DA+ALLR+++L+VESFEIKDVKTLRG+H SRAIGRL+
Sbjct: 113 PDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDVKTLRGDHQSRAIGRLA 172

Query: 157 GKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAV 216
           GK G+TKF IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LRAV
Sbjct: 173 GKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKVYGNLRAV 232

Query: 217 TARLAER 224
            +RL+ER
Sbjct: 233 ASRLSER 239

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BLAST of Carg23536 vs. Swiss-Prot
Match: sp|Q29IG6|PNO1_DROPS (RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l(1)G0004 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 4.3e-65
Identity = 120/178 (67.42%), Postives = 153/178 (85.96%), Query Frame = 0


Query: 46  QFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKC 105
           + RKVSVPPHRYS LK+ WM+I+TP+ E MK+ IR N+KAR+VEL+   +TPDI+NLQK 
Sbjct: 60  ELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPETPDIANLQKG 119

Query: 106 ADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFA 165
           ADFV AF+ GF+V DA+ALLR+++L+VE+FEIKDVKTLRG+H SRAIGRL+GK G+TKF 
Sbjct: 120 ADFVKAFLCGFEVDDALALLRLEDLFVETFEIKDVKTLRGDHQSRAIGRLAGKGGRTKFT 179

Query: 166 IENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAER 224
           IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LR+V +RL+ER
Sbjct: 180 IENVTKTRIVLADSKIHILGSYQNIQLARRAICNLILGSPPSKVYGNLRSVASRLSER 237

[Close]

BLAST of Carg23536 vs. Swiss-Prot
Match: sp|A7RP64|PNO1_NEMVE (RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.6e-65
Identity = 119/196 (60.71%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 29  KPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKV 88
           +P F P+K  ++  G+ + RK+ VP HRY+PLK+ WM+I+TP+ E +K+ IR NL +R V
Sbjct: 43  RPSFPPVKAQKLAGGKSETRKIPVPSHRYTPLKENWMKIFTPVVEHLKLQIRFNLGSRHV 102

Query: 89  ELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHL 148
           E++   +T DI  +QK ADFV AF+LGF+V DA+AL+R+D+L++ESFEI DVK L+G+HL
Sbjct: 103 EIRASKETSDIGAVQKAADFVQAFILGFEVEDALALIRLDDLFLESFEIADVKPLKGDHL 162

Query: 149 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 208
           SRAIGR++GK GKTKF IEN TKTRIV+A+TKIHILGSF NIKIAR ++C+LILGSP  K
Sbjct: 163 SRAIGRVAGKGGKTKFTIENVTKTRIVLAETKIHILGSFQNIKIARTAICNLILGSPPSK 222

Query: 209 VYSKLRAVTARLAERF 225
           VY  +RAV +R AERF
Sbjct: 223 VYGNMRAVASRSAERF 238

[Close]

BLAST of Carg23536 vs. Swiss-Prot
Match: sp|Q6VBQ6|PNO1_ORYLA (RNA-binding protein PNO1 OS=Oryzias latipes OX=8090 GN=pno1 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-64
Identity = 119/196 (60.71%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 29  KPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDIRMNLKARKV 88
           +PHF P+   ++  G  + RKV+VP HRY+PLK+ W++I+TPI E +++ +R NLK R V
Sbjct: 49  RPHFPPISADKLR-GPDEMRKVAVPAHRYTPLKENWLKIFTPIVENLQLQVRFNLKTRNV 108

Query: 89  ELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVESFEIKDVKTLRGEHL 148
           E+KT  +T DI+ L K ADF+ AF+LGF V DA+AL+R+DEL++ESF++ DVK L+G+HL
Sbjct: 109 EIKTCKETQDIAALTKAADFIKAFVLGFQVDDAMALIRLDELFLESFDVTDVKPLKGDHL 168

Query: 149 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 208
           SRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+AR ++C+LILGSP  K
Sbjct: 169 SRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMARTAICNLILGSPPSK 228

Query: 209 VYSKLRAVTARLAERF 225
           VY  +RAV +R AERF
Sbjct: 229 VYGNIRAVASRTAERF 243

[Close]

BLAST of Carg23536 vs. Swiss-Prot
Match: sp|Q6VEU3|PNO1_DANRE (RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 6.2e-64
Identity = 123/211 (58.29%), Postives = 161/211 (76.30%), Query Frame = 0


Query: 15  VSSQPKPVVSTLPPK-PHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYE 74
           + +Q +   S+ P K PHF  L   ++  G  + RKV VP HRY+PLK+ W++I+TPI E
Sbjct: 42  MDTQTEESSSSAPVKRPHFPALSGDQLGGGVDEMRKVPVPSHRYTPLKENWLKIFTPIVE 101

Query: 75  QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAVALLRVDELYVE 134
            +++ +R NLK R+VE+KT  +T DI  L + ADFV AF+LGF V DA+AL+R+DEL++E
Sbjct: 102 NLQLQVRFNLKTRQVEIKTCKETQDIGALTRAADFVKAFVLGFQVEDALALIRLDELFLE 161

Query: 135 SFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIA 194
           +F++ DVK L+G+HLSRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+A
Sbjct: 162 TFDVTDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMA 221

Query: 195 RDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           R ++C+LILGSP  KVY  LRAV  R AERF
Sbjct: 222 RTAICNLILGSPPSKVYGNLRAVATRSAERF 252

[Close]

BLAST of Carg23536 vs. TrEMBL
Match: tr|A0A1S3BLP2|A0A1S3BLP2_CUCME (RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 2.8e-114
Identity = 215/224 (95.98%), Postives = 220/224 (98.21%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTEASTSMEVE +S+Q K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIY+QMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. TrEMBL
Match: tr|A0A0A0L7D9|A0A0A0L7D9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045150 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 5.9e-112
Identity = 212/224 (94.64%), Postives = 218/224 (97.32%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EASTSM+VE +S+Q K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSNEASTSMDVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWM+IYTPIY+QMKIDIRMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFDVID
Sbjct: 61  KKAWMDIYTPIYDQMKIDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. TrEMBL
Match: tr|A0A0A0KQC5|A0A0A0KQC5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139880 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.5e-110
Identity = 209/224 (93.30%), Postives = 214/224 (95.54%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTE   SMEVE VSS+    + TLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEVPVSMEVETVSSKTVTEIGTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIY+QMKIDIRMNLKARKVELKTRADTPD+SNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRADTPDVSNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. TrEMBL
Match: tr|A0A1S3AUA6|A0A1S3AUA6_CUCME (RNA-binding protein pno1-like OS=Cucumis melo OX=3656 GN=LOC103482927 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-109
Identity = 208/224 (92.86%), Postives = 214/224 (95.54%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQSTE   SMEVE VSS+    V T+PPKP F+PLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEVPVSMEVETVSSKTVTEVGTMPPKPQFEPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KKAWMEIYTPIY+QMKIDIRMNLKARKVELKTRA+TPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDIRMNLKARKVELKTRAETPDISNLQKCADFVHAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

[Close]

BLAST of Carg23536 vs. TrEMBL
Match: tr|A0A251NL97|A0A251NL97_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G032300 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 5.4e-105
Identity = 198/224 (88.39%), Postives = 209/224 (93.30%), Query Frame = 0


Query: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60
           MQS EA  SMEVEAV S+P    + LPPKP F+PLK HEM+DGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSNEAPVSMEVEAVPSKPSSASAALPPKPIFEPLKAHEMSDGRVQFRKVSVPPHRYSPL 60

Query: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120
           KK WMEIYTP+YEQMKIDIRMNLK RKVELK+RADTPD+SNLQKC+DFV AFMLGFDVID
Sbjct: 61  KKVWMEIYTPVYEQMKIDIRMNLKGRKVELKSRADTPDVSNLQKCSDFVQAFMLGFDVID 120

Query: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180
           A+ALLR+DELYVESFEIKDVKTLRGEHLSRAIGRLSGK GKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRMDELYVESFEIKDVKTLRGEHLSRAIGRLSGKGGKTKFAIENATKTRIVIADTK 180

Query: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF 225
           IHILGSFANIK+ARDSLCSLILGSPAGKVYSKLRAVTARLAERF
Sbjct: 181 IHILGSFANIKVARDSLCSLILGSPAGKVYSKLRAVTARLAERF 224

The following BLAST results are available for this feature:

BLAST of Carg23536 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951370.1	2.2e-118	100.00	RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >XP_022951376.1 RN...	[more]
XP_023005535.1	6.4e-118	99.11	RNA-binding protein pno1-like isoform X1 [Cucurbita maxima] >XP_023005536.1 RNA-...	[more]
XP_023540523.1	1.1e-117	98.66	RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540...	[more]
XP_022951385.1	3.3e-114	100.00	RNA-binding protein pno1-like isoform X2 [Cucurbita moschata]	[more]
XP_008449001.1	4.3e-114	95.98	PREDICTED: RNA-binding protein pno1 [Cucumis melo]	[more]

BLAST of Carg23536 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G13230.1	1.9e-95	80.56	RNA-binding KH domain-containing protein	[more]

BLAST of Carg23536 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9VR89\|PNO1_DROME	3.9e-66	65.78	RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV...	[more]
sp\|Q29IG6\|PNO1_DROPS	4.3e-65	67.42	RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l...	[more]
sp\|A7RP64\|PNO1_NEMVE	5.6e-65	60.71	RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1	[more]
sp\|Q6VBQ6\|PNO1_ORYLA	1.2e-64	60.71	RNA-binding protein PNO1 OS=Oryzias latipes OX=8090 GN=pno1 PE=2 SV=1	[more]
sp\|Q6VEU3\|PNO1_DANRE	6.2e-64	58.29	RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2	[more]

BLAST of Carg23536 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BLP2\|A0A1S3BLP2_CUCME	2.8e-114	95.98	RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=4 SV=1	[more]
tr\|A0A0A0L7D9\|A0A0A0L7D9_CUCSA	5.9e-112	94.64	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045150 PE=4 SV=1	[more]
tr\|A0A0A0KQC5\|A0A0A0KQC5_CUCSA	1.5e-110	93.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139880 PE=4 SV=1	[more]
tr\|A0A1S3AUA6\|A0A1S3AUA6_CUCME	1.2e-109	92.86	RNA-binding protein pno1-like OS=Cucumis melo OX=3656 GN=LOC103482927 PE=4 SV=1	[more]
tr\|A0A251NL97\|A0A251NL97_PRUPE	5.4e-105	88.39	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G032300 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0003676	nucleic acid binding

Vocabulary: **INTERPRO**
Term	Definition
IPR036612	KH_dom_type_1_sf
IPR004087	KH_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23536-RA	Carg23536-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004087	K Homology domain	SMART	SM00322	kh_6	coord: 129..202 e-value: 1.7E-4 score: 31.0
IPR036612	K Homology domain, type 1 superfamily	GENE3D	G3DSA:3.30.1370.10		coord: 128..224 e-value: 1.7E-24 score: 87.8
IPR036612	K Homology domain, type 1 superfamily	SUPERFAMILY	SSF54791	Eukaryotic type KH-domain (KH-domain type I)	coord: 129..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..38
None	No IPR available	PANTHER	PTHR12826	FAMILY NOT NAMED	coord: 20..224
None	No IPR available	PANTHER	PTHR12826:SF13	RNA-BINDING PROTEIN PNO1	coord: 20..224
None	No IPR available	CDD	cd00105	KH-I	coord: 149..195 e-value: 0.00871914 score: 32.1468

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg23536	Cla004443	Watermelon (97103) v1	carwmB0010
Carg23536	Csa3G045150	Cucumber (Chinese Long) v2	carcuB0008
Carg23536	MELO3C014081	Melon (DHL92) v3.5.1	carmeB0006
Carg23536	ClCG10G004100	Watermelon (Charleston Gray)	carwcgB0008
Carg23536	CSPI03G03920	Wild cucumber (PI 183967)	carcpiB0008
Carg23536	Cucsa.041830	Cucumber (Gy14) v1	carcgyB0007
Carg23536	CmaCh06G006890	Cucurbita maxima (Rimu)	carcmaB0013
Carg23536	CmaCh16G009770	Cucurbita maxima (Rimu)	carcmaB0012
Carg23536	Cp4.1LG14g07990	Cucurbita pepo (Zucchini)	carcpeB0010
Carg23536	CsGy3G003920	Cucumber (Gy14) v2	carcgybB0007
Carg23536	MELO3C014081.2	Melon (DHL92) v3.6.1	carmedB0007
Carg23536	Cla97C10G188570	Watermelon (97103) v2	carwmbB0007
Carg23536	Bhi11G000341	Wax gourd	carwgoB0009

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg23536	Cucurbita moschata (Rifu)	carcmoB0012
Carg23536	Cucurbita moschata (Rifu)	carcmoB0013
Carg23536	Cucurbita pepo (Zucchini)	carcpeB0011
Carg23536	Cucumber (Chinese Long) v3	carcucB0008