Carg23491 (gene) Silver-seed gourd

BLAST of Carg23491 vs. NCBI nr
Match: XP_022956242.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 812.8 bits (2098), Expect = 5.3e-232
Identity = 397/397 (100.00%), Postives = 397/397 (100.00%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60
           MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN
Sbjct: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120
           PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180
           TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND
Sbjct: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180

Query: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240
           VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP
Sbjct: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 398
           VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH
Sbjct: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 397

BLAST of Carg23491 vs. NCBI nr
Match: XP_022979578.1 (probable polygalacturonase At3g15720 [Cucurbita maxima])

HSP 1 Score: 770.0 bits (1987), Expect = 3.9e-219
Identity = 373/397 (93.95%), Postives = 387/397 (97.48%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60
           MKL VSFVFLF   VLSSLTES SSYFNVLDYGA+GNGK DDSEAFLKAWNDACASTEDN
Sbjct: 1   MKLLVSFVFLFSTAVLSSLTESTSSYFNVLDYGANGNGKKDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120
           PTLRVP GKTFLLNPLKFQGPCKS+RINFDLGGIVMAPSKDEWS+DGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPFGKTFLLNPLKFQGPCKSERINFDLGGIVMAPSKDEWSNDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180
           TVKGDG+FDGQGSSWWDKDCHKHCKRPTA+FFHNCN LHL+KTKHVNSGRNHMSIN+CND
Sbjct: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTALFFHNCNGLHLNKTKHVNSGRNHMSINNCND 180

Query: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240
           VIISDIHITAP+ESPNTDGIDISGSKNVRI DSFIGTGDDC+AINDGSSNIDIIGLTCGP
Sbjct: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+GDYNVVENVYVS+CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKDGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYTAYAY RKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYTAYAYRRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 398
           VNITTS+S+EEAKSFCQNADG+STSTVPPVPCLSKSH
Sbjct: 361 VNITTSSSNEEAKSFCQNADGKSTSTVPPVPCLSKSH 397

BLAST of Carg23491 vs. NCBI nr
Match: XP_023526924.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 750.0 bits (1935), Expect = 4.2e-213
Identity = 368/397 (92.70%), Postives = 377/397 (94.96%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60
           MKL VSFVFLF I VLSSLTESASSYFNVLDYGA+GNGK DDSEAFLKAWND CASTEDN
Sbjct: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120
           PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGG VMAP+KDEWS DGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180
           TVKGDG+FDGQGSSWWDKDCHKHCKRPTAIFFHNCN LHLSKTKHVNSG+NHMSIN CND
Sbjct: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180

Query: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240
           VIISDIHITAP+ESPNTDGIDISGSKNVRI DSFIGTGDDC+AINDGSSNIDI GLTCGP
Sbjct: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGKNGDYNVVENVYVS+CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYT         GREIKVSDVTYR+VHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYT---------GREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 398
           VNITTSTSDEEAKSFCQNADG+STSTVPPVPCLSKSH
Sbjct: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 388

BLAST of Carg23491 vs. NCBI nr
Match: XP_023552838.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.1 bits (1468), Expect = 5.9e-159
Identity = 277/397 (69.77%), Postives = 312/397 (78.59%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60
           MKL  + +FLF++ VL SLT SA SYFNVLDYGA GNGKTDDSEAFLKAWNDACA  EDN
Sbjct: 1   MKLLFAVIFLFYVTVLRSLTVSALSYFNVLDYGATGNGKTDDSEAFLKAWNDACAVIEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120
           PTLRVP GKT+LL PLKFQGPCKSK++NFDLGG +MAPSKDEW SDG DKW+QFY+IDSL
Sbjct: 61  PTLRVPFGKTYLLKPLKFQGPCKSKQVNFDLGGALMAPSKDEWPSDGEDKWVQFYNIDSL 120

Query: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180
           TV+GDG+F+GQGSSWW+KDCH HC RPTA+FFHNCN L LSK KH+NSG+NH+SIN CN+
Sbjct: 121 TVQGDGRFNGQGSSWWNKDCHNHCNRPTALFFHNCNGLRLSKMKHINSGKNHISINDCNN 180

Query: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240
           V+ISDIHI+AP +SPNTDGIDIS SKNV I +S I TGDDCIAIN+GSSNI+I+G+TCGP
Sbjct: 181 VVISDIHISAPEQSPNTDGIDISQSKNVLIQNSLIATGDDCIAINNGSSNINIVGITCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+GDYNVVEN+YV  C L+DTQNGVRIKTW                    
Sbjct: 241 GHGISIGSLGKDGDYNVVENIYVRNCHLKDTQNGVRIKTWXXXXXXXXXXXXXXXXXXXX 300

Query: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360
                                   KVS+VTYR+VHGTSANE AI L+CSRARCTNIIM  
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXKVSNVTYREVHGTSANENAIILNCSRARCTNIIMNN 360

Query: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 398
           VNI TST D EAKS CQNADG + S +PPVPCLSKSH
Sbjct: 361 VNIRTSTPDTEAKSVCQNADGRAISVIPPVPCLSKSH 397

BLAST of Carg23491 vs. NCBI nr
Match: XP_022141108.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 269/397 (67.76%), Postives = 321/397 (80.86%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60
           MKL ++ VF+F I +LSS   S S  FNVLDYGA GNG+TDDS+AFL+AW DAC +TE  
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120
           P L  P GK ++LNPLKFQGPCKSK++ F LGG V+AP+KD W SD  D+WIQF DID L
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180
            V G GQFDGQGSSWW KDC KHC RPTA+F HNCN L L + KH+NSGRNH+SIN+CND
Sbjct: 121 MVNGSGQFDGQGSSWW-KDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACND 180

Query: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240
            IISD+HI AP +SPNTDGIDISGS NV I +SF+GTGDDCIA+N+GS+NI+I+G+TCGP
Sbjct: 181 AIISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLG+NG YNVVE+++V  C+L+ TQNGVRIKTW+ GYGYA+ ITFE+ITL +V
Sbjct: 241 GHGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDV 300

Query: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQ+Y+  A  +KN+   IKVSDVTYR+VHGTSANE AI L+CSR+RC+NIIME 
Sbjct: 301 KNPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMEN 360

Query: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 398
           V+I      +EAK  CQN DG STS +P VPCLSK +
Sbjct: 361 VSIKNIDPXKEAKXLCQNVDGRSTSAIPSVPCLSKQN 396

BLAST of Carg23491 vs. TAIR10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 350.1 bits (897), Expect = 1.8e-96
Identity = 194/392 (49.49%), Postives = 247/392 (63.01%), Query Frame = 0

Query: 10  LFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGK 69
           LFF+ + +S     S+  +V  +GA G+G TDDS+AFLKAW   C+ T D   + VP+G 
Sbjct: 12  LFFLQIFTS-----SNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFV-VPAGM 71

Query: 70  TFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSLTVKGDGQFD 129
           TF+L PLKFQG CKS  +   + G ++APSK  W  D  D+WI F DI+ L ++GDG+ +
Sbjct: 72  TFMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEIN 131

Query: 130 GQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHIT 189
           GQGSSWW+   HK   RPTA+ F +CN L LS   H++S   H+ I+ CN V IS + I 
Sbjct: 132 GQGSSWWE---HKG-SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRIN 191

Query: 190 APNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSL 249
           AP  SPNTDGID+  S NV I D  I TGDDCIAIN G+SNI I G+ CGPGHGISIGSL
Sbjct: 192 APESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSL 251

Query: 250 GKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQH 309
           GK+G+   VENV V  C  + T NG RIKTW+GG GYA+ ITF  ITL+NV+NPIIIDQ 
Sbjct: 252 GKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQF 311

Query: 310 YTA--YAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCS-RARCTNIIMEEVNITT- 369
           Y        +  K   ++VS V + +  GTS +E  +   CS R  CT I + ++ I T 
Sbjct: 312 YNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETA 371

Query: 370 -STSDEEAKSFCQNADGESTSTVPPVPCLSKS 397
            S S + A+  C N  G ST  VP + CL  S
Sbjct: 372 SSGSGQVAQGQCLNVRGASTIAVPGLECLELS 392

BLAST of Carg23491 vs. TAIR10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 349.4 bits (895), Expect = 3.0e-96
Identity = 183/362 (50.55%), Postives = 236/362 (65.19%), Query Frame = 0

Query: 27  FNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKR 86
           +NVL++ A G+G+TDDSEAFL+AW  AC    D  TL +PSGKTFLL P  FQGPCKS  
Sbjct: 24  YNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQGPCKSSS 83

Query: 87  INFDLGGIVMAPS-KDEWSSDGVDKWIQFYDIDSLTVKGDGQFDGQGSSWWDKDCHKHCK 146
           I   L G ++APS K  WS      WI+F  +  L + G G  D +GSS+W+ +  K  +
Sbjct: 84  IKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELNL-KASQ 143

Query: 147 RPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPNESPNTDGIDISGS 206
           RPTA+    C+ L ++    ++S +NH+SI +CN V IS+I++ AP  SPNTDGIDIS S
Sbjct: 144 RPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPNTDGIDISDS 203

Query: 207 KNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKNGDYNVVENVYVSE 266
            N+ I DS I TGDDCIAIN GSSNI+I G+ CGPGHGIS+GSLG  G    V +V V+ 
Sbjct: 204 TNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEAKVSDVQVTH 263

Query: 267 CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTAYAYGRKNKGREIK 326
           C    T NG RIKTW GG GYA+NI+F  ITL N KNPIIIDQHY     GR  +   + 
Sbjct: 264 CTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHY--IDKGRLTEESAVA 323

Query: 327 VSDVTYRDVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEEAKSFCQNADGEST 386
           +S+V + D  GTS+N+ AITL CS    C +++M+ ++IT +    + K  CQ  DGES+
Sbjct: 324 ISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITMANGG-KPKVNCQYVDGESS 381

BLAST of Carg23491 vs. TAIR10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 339.3 bits (869), Expect = 3.1e-93
Identity = 174/360 (48.33%), Postives = 235/360 (65.28%), Query Frame = 0

Query: 27  FNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKR 86
           +NVL +GA+G+G+TDDS AFL+AWN  C   E+  TL +PSGKT+LL P++F+GPCKS  
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 87  INFDLGGIVMAPSK-DEWSSDGVDKWIQFYDIDSLTVKGDGQFDGQGSSWWDKDCHKHCK 146
           I   L GI++APS    WS+     WI F  +  L + G G  +G+GSS+W+        
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWE-------- 172

Query: 147 RPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPNESPNTDGIDISGS 206
              A+   NCN L ++    ++S ++H+SI +C+ V IS I+I AP  SPNTDGIDIS S
Sbjct: 173 ---ALHICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYS 232

Query: 207 KNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKNGDYNVVENVYVSE 266
            NV I DS I TGDDCIAIN GSS+I+I  + CGPGHGIS+GSLG +G+   V +VYV++
Sbjct: 233 TNVNIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQ 292

Query: 267 CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTAYAYGRKNKGREIK 326
           C    T NG RIKTW+GG GYA+NI+FE ITL NV+NPIIIDQ YT        K   + 
Sbjct: 293 CTFNKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVA 352

Query: 327 VSDVTYRDVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEEAKSFCQNADGEST 385
           +S V Y    GT+ NE AI L CS    C +++++++ +T   + E+ K  C+N +GES+
Sbjct: 353 ISSVKYVGFQGTTLNEDAIMLKCSAITYCKDMVIDDIEVTME-NGEKPKVECENVEGESS 400

BLAST of Carg23491 vs. TAIR10
Match: AT5G17200.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 318.2 bits (814), Expect = 7.4e-87
Identity = 169/381 (44.36%), Postives = 231/381 (60.63%), Query Frame = 0

Query: 23  ASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGKTFLLNPLKFQGPC 82
           ++ + +V  +GA G+GKTDD++AFLKAW   C     N  + VP GKTF+L PL F GPC
Sbjct: 35  SNGFISVTSFGAIGDGKTDDTKAFLKAWEAVCKGGH-NRKILVPQGKTFMLKPLTFIGPC 94

Query: 83  KSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSLTVKGDGQFDGQGSSWWDKDCHK 142
           KS  I+  + G ++AP    W +     WI F  I+ L V G G  DG+GS WW    + 
Sbjct: 95  KSSTISLSIRGNLVAPGY-TWYAGRYTTWISFDSINGLVVTGGGTIDGRGSLWWGNVNN- 154

Query: 143 HCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPNESPNTDGIDI 202
              RP A+ F+NCN L +S  +H+NS RNH+ ++   ++ +  + +TAP +SPNTDGIDI
Sbjct: 155 ---RPCAMHFNNCNGLRISNLRHLNSPRNHVGLSCSQNIEVRGLRMTAPGDSPNTDGIDI 214

Query: 203 SGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKNGDYNVVENVY 262
           S    V I DS I TGDDCIAIN GSS+I+I G+ CGPGHGIS+GSLG  GD+  VE V 
Sbjct: 215 SNCIGVHIHDSVIATGDDCIAINSGSSHINITGIFCGPGHGISVGSLGVTGDFETVEEVR 274

Query: 263 VSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQ-------------- 322
           V  C    TQNGVRIKT++ G GYA+ I+FE I +   +NPIIIDQ              
Sbjct: 275 VKNCTFTKTQNGVRIKTYQNGSGYARKISFEDINMVASENPIIIDQTYHNGGTNGGISKS 334

Query: 323 -------HYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDC-SRARCTNIIMEE 382
                  H TA        G+ +KV+DV Y  + G+SA+++ ITL+C +   C++I+M+ 
Sbjct: 335 SSSYQNCHLTAKQRTPSGNGKGVKVTDVRYARIRGSSASDQDITLNCDADLGCSDIVMDN 394

BLAST of Carg23491 vs. TAIR10
Match: AT5G44840.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 318.2 bits (814), Expect = 7.4e-87
Identity = 169/382 (44.24%), Postives = 241/382 (63.09%), Query Frame = 0

Query: 10  LFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGK 69
           +F + +L S+  +   Y +VL++GADGNG TDDS+AF+KAW+  C S  ++ T  +PS K
Sbjct: 8   VFLLTLLHSILVNGQIY-DVLEFGADGNGITDDSKAFVKAWSAMCGSGGNSKTFIIPSNK 67

Query: 70  TFLLNPLKFQGPCKSKRINFDLGGIVMAP-SKDEWSSDGVDKWIQFYDIDSLTVKGDGQF 129
           TFLL PL FQGPCKS  +     G ++AP +K  WS   + +W+ F +I  LTV G G  
Sbjct: 68  TFLLQPLTFQGPCKSPSVQVKFDGKIVAPINKAAWSDYKLFRWVSFKEIIGLTVNGSGTI 127

Query: 130 DGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHI 189
            G+GSS+W +           + F  CN L ++     NS +NH+SI+ C  V I+ I +
Sbjct: 128 HGRGSSFWKQ-----------LHFQRCNDLMITGITSFNSPKNHISISECKRVKITKIKL 187

Query: 190 TAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGS 249
            AP++SPNTDGI+IS S +V I D+ IGTGDDC+AIN GS NI+I  + CGPGHGIS+GS
Sbjct: 188 VAPHDSPNTDGINISESSDVDIYDTVIGTGDDCVAINSGSMNINIARMNCGPGHGISVGS 247

Query: 250 LGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQ 309
           +G++G+ ++VENV V+ C    T NG RIKTW  G GYAKNI F+ +T    KNPIIIDQ
Sbjct: 248 VGRDGEESIVENVQVTNCTFIRTDNGARIKTWPNGKGYAKNILFKSLTFRETKNPIIIDQ 307

Query: 310 HYTAYAYGRKNKGR------EIKVSDVTYRDVHGTSANEKAITLDCSRAR-CTNIIMEEV 369
           +Y        +KGR       + +S+VT+ D+ GTS  ++ I +DCS+   C +I+++++
Sbjct: 308 NYV-------DKGRLDVEESAVAISNVTFTDLRGTSKLDEIIKIDCSKVTYCKDIVLDKI 367

Query: 370 NITTSTSDEEAKSFCQNADGES 384
           +I T   ++     C N  G+S
Sbjct: 368 DIATVDGNKPIVE-CSNVYGKS 369

BLAST of Carg23491 vs. Swiss-Prot
Match: sp|Q9LW07|PGLR3_ARATH (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 3.2e-95
Identity = 194/392 (49.49%), Postives = 247/392 (63.01%), Query Frame = 0

Query: 10  LFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGK 69
           LFF+ + +S     S+  +V  +GA G+G TDDS+AFLKAW   C+ T D   + VP+G 
Sbjct: 12  LFFLQIFTS-----SNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFV-VPAGM 71

Query: 70  TFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSLTVKGDGQFD 129
           TF+L PLKFQG CKS  +   + G ++APSK  W  D  D+WI F DI+ L ++GDG+ +
Sbjct: 72  TFMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEIN 131

Query: 130 GQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHIT 189
           GQGSSWW+   HK   RPTA+ F +CN L LS   H++S   H+ I+ CN V IS + I 
Sbjct: 132 GQGSSWWE---HKG-SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRIN 191

Query: 190 APNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSL 249
           AP  SPNTDGID+  S NV I D  I TGDDCIAIN G+SNI I G+ CGPGHGISIGSL
Sbjct: 192 APESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSL 251

Query: 250 GKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQH 309
           GK+G+   VENV V  C  + T NG RIKTW+GG GYA+ ITF  ITL+NV+NPIIIDQ 
Sbjct: 252 GKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQF 311

Query: 310 YTA--YAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCS-RARCTNIIMEEVNITT- 369
           Y        +  K   ++VS V + +  GTS +E  +   CS R  CT I + ++ I T 
Sbjct: 312 YNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETA 371

Query: 370 -STSDEEAKSFCQNADGESTSTVPPVPCLSKS 397
            S S + A+  C N  G ST  VP + CL  S
Sbjct: 372 SSGSGQVAQGQCLNVRGASTIAVPGLECLELS 392

BLAST of Carg23491 vs. Swiss-Prot
Match: sp|Q94AJ5|PGLR5_ARATH (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 3.2e-79
Identity = 156/374 (41.71%), Postives = 224/374 (59.89%), Query Frame = 0

Query: 28  NVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKRI 87
           +V ++GA GNG TDD++AF  AW  AC+S      L VP   T LL P+   GPCK+ R+
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRIL-VPENYTCLLRPIDLSGPCKA-RL 113

Query: 88  NFDLGGIVMAPS-KDEWSSDGVDKWIQFYDIDSLTVKGDGQFDGQGSSWWDKDC-HKH-- 147
              + G ++AP+  D W      KW+ F+ +  LTV+G G  +G G  WW++ C H H  
Sbjct: 114 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 173

Query: 148 -CK-RPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPNESPNTDGID 207
            C+  PTA+ FH C  + +     ++S + H+++ SC  V IS + + AP  SPNTDGI 
Sbjct: 174 PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIH 233

Query: 208 ISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKNGDYNVVENV 267
           IS S+ + I ++ + TGDDCI+I   S+ I I  + CGPGHGISIGSLGK+  +  V ++
Sbjct: 234 ISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDI 293

Query: 268 YVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTAYAYGRKNKG 327
            V   I+ DT NGVRIKTW+GG G    I F  I + NV NPIIIDQ+Y        N+ 
Sbjct: 294 TVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQT 353

Query: 328 REIKVSDVTYRDVHGTSANEKAITLDCS-RARCTNIIMEEVNITTSTSDEEAKSFCQNAD 387
             I + ++++  V GTSA+++AI + CS  + C NI+++++++  S  D   +SFC  A 
Sbjct: 354 SAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAY 413

Query: 388 GESTSTVPPVPCLS 395
           G S+  V P PCLS
Sbjct: 414 GSSSGQVYPPPCLS 425

BLAST of Carg23491 vs. Swiss-Prot
Match: sp|Q02096|PGLR_PERAE (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.9e-76
Identity = 157/363 (43.25%), Postives = 215/363 (59.23%), Query Frame = 0

Query: 22  SASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGKTFLLNPLKFQGP 81
           S  +  +V D+GA G+G TDD++AF KAW DAC+S      L VP  K +LL  + F GP
Sbjct: 80  SPDTDISVDDFGARGDG-TDDTKAFEKAWKDACSS---GSVLIVPENKNYLLKQITFSGP 139

Query: 82  CKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSLTVKGDGQFDGQGSSWWDKDCH 141
           CKS       G I  +  + +W      +WI+F DI +LT++G G  +G G +WWD  C 
Sbjct: 140 CKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCK 199

Query: 142 KH----CK-RPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPNESPN 201
           +     CK  PTA+ F +C  L +S     +S + H+S + C DVI S++ +TAP  SPN
Sbjct: 200 RKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPN 259

Query: 202 TDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKNGDYN 261
           TDGI I+G++ + +++S IGTGDDCI+I  GS  +    +TCGPGHGISIGSLG      
Sbjct: 260 TDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEA 319

Query: 262 VVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTAYAYG 321
            V  V V    L DT NG+RIKTW+GG G AKNI F+ I + NV NPIIIDQ+Y      
Sbjct: 320 HVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDP 379

Query: 322 RKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRAR-CTNIIMEEVNITTSTSDEEAKSF 379
              +   +KVS+V Y ++ GTSA+E A+  DCS++  C   I+  +N+  +   E   S 
Sbjct: 380 CPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKETTMS- 437

BLAST of Carg23491 vs. Swiss-Prot
Match: sp|Q8RY29|ADPG2_ARATH (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 283.9 bits (725), Expect = 2.8e-75
Identity = 159/370 (42.97%), Postives = 222/370 (60.00%), Query Frame = 0

Query: 28  NVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKRI 87
           +V D+GA G+GKTDD++AF+ AW  AC S+     L VP G T+LL  ++  GPC S  +
Sbjct: 69  SVSDFGAKGDGKTDDTQAFVNAWKKAC-SSNGAVNLLVPKGNTYLLKSIQLTGPCNS-IL 128

Query: 88  NFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSLTVKG--DGQFDGQGSSWWDKDCHKH-- 147
              + G + A  K     D + KWI F  +++L+V G   G  DG G +WW   C ++  
Sbjct: 129 TVQIFGTLSASQKRSDYKD-ISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKA 188

Query: 148 ---CKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPNESPNTDGI 207
               K PTA+ F+N   L +   K  N+ +  +SI  C++V +S++ +TAP +SPNTDGI
Sbjct: 189 KPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGI 248

Query: 208 DISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKNGDYNVVEN 267
            I+ ++N+R+ +S IGTGDDCI+I  GS N+ I  +TCGPGHGISIGSLG +     V  
Sbjct: 249 HITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSG 308

Query: 268 VYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTAYAYGRKNK 327
           V V    L  T NGVRIKT++GG G A NI F+ I ++NVKNPIIIDQ Y   +     K
Sbjct: 309 VTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEK 368

Query: 328 GREIKVSDVTYRDVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEEAKSFCQNA 387
              ++V +V YRD+ GTSA+E AIT +CS+   C  I+++ VNI      +  K+ C NA
Sbjct: 369 S-AVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI------KGGKATCTNA 428

Query: 388 DGESTSTVPP 390
           +      V P
Sbjct: 429 NVVDKGAVLP 428

BLAST of Carg23491 vs. Swiss-Prot
Match: sp|Q9SFB7|QRT2_ARATH (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-73
Identity = 152/379 (40.11%), Postives = 222/379 (58.58%), Query Frame = 0

Query: 22  SASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVPSGKTFLLNPLKFQGP 81
           S+   FNV  +GA  NG  DDS+AF+KAW  AC+ST     +  P  + ++L  + F GP
Sbjct: 66  SSPRSFNVNTFGAKANG-NDDSKAFMKAWEAACSST-GIVYIVAPKNRDYMLKAVTFSGP 125

Query: 82  CKSKRINFDLGGIVMAPSKDEWSSDGVDK----WIQFYDIDSLTVKGDGQFDGQGSSWWD 141
           CKS  I F + G + A     W +    K    WI F ++++L V+G G+ DG G  WW 
Sbjct: 126 CKSSLIIFKIYGRIEA-----WENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWP 185

Query: 142 KDCHKHCK-----RPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPN 201
           K C  + +      PTA+ F  CN L +S  +  N+ + H++   C +V   ++ +T+P 
Sbjct: 186 KSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPA 245

Query: 202 ESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKN 261
           +SPNTDGI +SG++N+ I DS + TGDDCI+I  GS N+   G+TCGPGHGISIGSLG++
Sbjct: 246 DSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGED 305

Query: 262 GDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTA 321
                V NV V++  L  T NGVRIKTW+GG+G AKNI F+ I ++NV NPIII+Q Y  
Sbjct: 306 NSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCD 365

Query: 322 YAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEE 381
                  +   ++VS+V Y+++ GTS+   A+   CS+   C  I M+ V +   T  + 
Sbjct: 366 RVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDV 425

Query: 382 AKSFCQNADGESTSTVPPV 391
           +K+ C N   ++   V P+
Sbjct: 426 SKASCSNVKLDTRGNVSPL 437

BLAST of Carg23491 vs. TrEMBL
Match: tr|A0A0A0KZ95|A0A0A0KZ95_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 6.5e-138
Identity = 249/398 (62.56%), Postives = 299/398 (75.13%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60
           MKL  + +FL+ I +LSS T   SS FNVLDYGA GNGKTDDS+AFLKAWND C +TE++
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120
           PTL VP GKT+LLNPLKFQGPCKSK+    LGG +MAP+KDEW SDG  +WIQF+DI+ L
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQ----LGGTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 TVKGDGQFDGQGSSWWDKDC-HKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCN 180
            ++G G+FDGQGS WW KDC HKHCKRPTA+FFHNCN L +   KH+NS +NH+SIN C+
Sbjct: 121 NLQGGGRFDGQGSLWW-KDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCH 180

Query: 181 DVIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCG 240
           DVI S +HI+AP +SPNTDGIDIS S N+ I DSF+ TGDDCIAIN+GSSNI+I G+TCG
Sbjct: 181 DVIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCG 240

Query: 241 PGHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLEN 300
           PGHGISIGSLGK+G+YNVVENV+VS C+L+ TQNG+RIKTWE                  
Sbjct: 241 PGHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEXXXXXXXXXXXXXXXXXX 300

Query: 301 VKNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIME 360
                               KG +IKVSDVTYR+V+GTSANE AIT +CS+ARC+NII++
Sbjct: 301 XXXXXXXXXXXXXXXXXXXMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILD 360

Query: 361 EVNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 398
            VNI  +   E+AK+ CQNADG+  S VPPV CLSKSH
Sbjct: 361 NVNIKMTNPGEKAKAVCQNADGKVLSAVPPVSCLSKSH 393

BLAST of Carg23491 vs. TrEMBL
Match: tr|A0A1S3BYT5|A0A1S3BYT5_CUCME (probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 4.5e-131
Identity = 235/397 (59.19%), Postives = 289/397 (72.80%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60
           MKL ++ +  + I +LSS T  +SS FNVLDYGA GNGKTDDS+AFLKAWND C +T+D+
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120
           PTL VP GKT+LL+PLKFQGPCKS ++ FDLGG +MAP+KDEW +DG  KW+QF D++ L
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180
            V+G G+FDGQGS WW            A+FFHNCN L ++  KHVNS +NH+SIN CND
Sbjct: 121 NVQGGGRFDGQGSCWW-----------KALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240
           VI S +HI+AP +SPNTDGIDIS S N+ I DSFIGTGDDC+AIN+GSSNI+I  +TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+G+YNVVENV+VS C+L++TQNG+RIKTW                    
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWXXXXXXXXXXXXXXXXXXXX 300

Query: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360
                               G +IKVSDVTYR+V+GTSANE+AIT +CS+ARC+NII++ 
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

Query: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 398
           VNI  +   EEAK+ CQN DG+  S VPPV CLSKSH
Sbjct: 361 VNIKMTNPGEEAKAVCQNVDGKVLSAVPPVSCLSKSH 386

BLAST of Carg23491 vs. TrEMBL
Match: tr|A0A2I4GY97|A0A2I4GY97_9ROSI (probable polygalacturonase At3g15720 OS=Juglans regia OX=51240 GN=LOC109011923 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 3.6e-120
Identity = 219/404 (54.21%), Postives = 292/404 (72.28%), Query Frame = 0

Query: 1   MKLFVSFVFLFFIVVLSS---------LTESASSYFNVLDYGADGNGKTDDSEAFLKAWN 60
           M+  + F+ L  I++L+          L+ +A   FNV+ YGA GNGKTDDS++FLKAW 
Sbjct: 1   MQGLIIFILLSCILILTPPLSSCARLLLSLAAGDTFNVIHYGAVGNGKTDDSQSFLKAWE 60

Query: 61  DACASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKW 120
           D C ++E  PTL +P+G TF+L+P+ F+GPCK+  ++  L G ++APS+D W  +G DKW
Sbjct: 61  DVCGASEGTPTLEIPNGMTFMLSPVAFKGPCKTNSVHVLLQGTIVAPSRDAW--NGNDKW 120

Query: 121 IQFYDIDSLTVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRN 180
           IQF +ID L + G G  DGQGSSWW +DCH +CKRPTA+  ++C+   L+  +H+NS +N
Sbjct: 121 IQFSEIDGLIIDGGGLIDGQGSSWW-QDCHSNCKRPTALHINDCHGSRLTGIRHLNSAKN 180

Query: 181 HMSINSCNDVIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNI 240
           H+SI SC D  ISD+ ITAP ESPNTDGIDIS S ++ I  S IGTGDDCIAIN G+SNI
Sbjct: 181 HISIASCTDFYISDLEITAPGESPNTDGIDISRSTSITIEKSKIGTGDDCIAINGGTSNI 240

Query: 241 DIIGLTCGPGHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNIT 300
            I  +TCGPGHGISIGSLG++G Y+ VE VYV  C  +  QNG RIKTW GG GYA+ IT
Sbjct: 241 KINQVTCGPGHGISIGSLGEDGSYDTVEEVYVRGCTFKGCQNGARIKTWAGGAGYARKIT 300

Query: 301 FEKITLENVKNPIIIDQHYTAY-AYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSR 360
           FE ITLE+ ++PIIIDQ Y+++      +KG  + VSDVTYRDV GTSA+++AI  +CS 
Sbjct: 301 FEDITLEDSQHPIIIDQFYSSHDGTQGPSKGSSVAVSDVTYRDVRGTSADDEAIIFNCSE 360

Query: 361 ARCTNIIMEEVNITTSTSDEEAKSFCQNADGESTSTVPPVPCLS 395
             C NI+M++++IT+S   ++A + C+NA+G +TSTVP VPCLS
Sbjct: 361 ESCKNIVMDQIDITSSVPGKDAHAVCKNAEGTATSTVPSVPCLS 401

BLAST of Carg23491 vs. TrEMBL
Match: tr|A0A2N9FAP3|A0A2N9FAP3_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11736 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 4.6e-115
Identity = 205/387 (52.97%), Postives = 274/387 (70.80%), Query Frame = 0

Query: 10  LFFIV---VLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDNPTLRVP 69
           +FFI    V +     A + FNV+DYGA G+G+TDDS+AFLKAW + C +++  PTL +P
Sbjct: 10  IFFITSPSVYARTIPQAQTIFNVIDYGAVGDGQTDDSQAFLKAWENVCGASQSTPTLNIP 69

Query: 70  SGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSLTVKGDG 129
            GKTF+L P++FQGPCKS  I F L G ++AP++D W  D  DKWIQF  +D L + G G
Sbjct: 70  QGKTFMLGPVEFQGPCKSNSIQFQLQGTLVAPTRDAWKGD--DKWIQFVYVDGLIINGGG 129

Query: 130 QFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDI 189
             DGQG+SWW+     +C RPTA+  H+CN   +++  H+NS RNH+SIN+C+ V +S +
Sbjct: 130 LIDGQGASWWESCI--NCDRPTALSIHSCNGSQITEISHLNSARNHISINACSGVHVSKL 189

Query: 190 HITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISI 249
            ITAP++SPNTDGIDIS S ++ I +S +GTGDDC+AIN G+ +I+I  + CGPGHGISI
Sbjct: 190 EITAPDDSPNTDGIDISHSNSITIENSTMGTGDDCVAINGGTYDINITNVVCGPGHGISI 249

Query: 250 GSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIII 309
           GSLG NG Y  VE+V+V  C+ + +QNGVRIKTW GG GYA+NI+FE+ITL   + PIII
Sbjct: 250 GSLGANGAYETVEDVHVKGCLFKGSQNGVRIKTWPGGSGYARNISFEQITLVGARRPIII 309

Query: 310 DQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEEVNITTS 369
           DQ+Y  ++  + +K   + VSDVTY D+HGTSA+EKAI LDCS   C NI+ E +NIT+S
Sbjct: 310 DQYY--FSSDKNSKKPSVVVSDVTYLDIHGTSADEKAIDLDCSDQGCKNIVFERINITSS 369

Query: 370 TSDEEAKSFCQNADGESTSTVPPVPCL 394
              +   + C NA G  TS+ P V CL
Sbjct: 370 IPGDNTYASCNNAYGSITSSFPSVSCL 390

BLAST of Carg23491 vs. TrEMBL
Match: tr|A0A1U8JJK3|A0A1U8JJK3_GOSHI (probable polygalacturonase At3g15720 OS=Gossypium hirsutum OX=3635 GN=LOC107907649 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.7e-109
Identity = 202/369 (54.74%), Postives = 257/369 (69.65%), Query Frame = 0

Query: 29  VLDYGADGNGKTDDSEAFLKAWNDACASTE--DNPTLRVPSGKTFLLNPLKFQGPCKSKR 88
           V+DYGA GNG+ DDS+AF KAW D C +    +  TL +P GKTFLLNPL FQGPCK+K 
Sbjct: 49  VMDYGATGNGQIDDSQAFNKAWKDICNANAGGETVTLEIPRGKTFLLNPLVFQGPCKAKA 108

Query: 89  INFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSLTVKGDGQFDGQGSSWWDKDCHKHCKR 148
           I   LGG ++AP    W+++GVD WIQF ++D+L + GDG+ DG+GS WW     + C +
Sbjct: 109 IRIQLGGTLIAPLSTSWANEGVDTWIQFSNVDNLYLDGDGKIDGRGSIWW-----QSCMK 168

Query: 149 PTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCNDVIISDIHITAPNESPNTDGIDISGSK 208
            +A+ F+NCN L L    H+NS R H+SI +C DVI+SD+ I+AP+ESPNTDGIDIS S 
Sbjct: 169 KSALHFNNCNGLQLKGLTHLNSPRAHISIKNCKDVIVSDLKISAPDESPNTDGIDISDST 228

Query: 209 NVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGPGHGISIGSLGKNGDYNVVENVYVSEC 268
           NV+IL S I TGDDC+AIN GSS I+I G+ CGPGHGISIGSLG  G Y+ VE V+V  C
Sbjct: 229 NVQILQSIIATGDDCVAINAGSSFINITGVVCGPGHGISIGSLGDGGAYDTVEQVHVKNC 288

Query: 269 ILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTAYAYGRKNKGREIKV 328
            L+DTQNGVRIKT++G  GYA+ I+FEKI L N +NPIII+Q +    Y    K   I++
Sbjct: 289 TLRDTQNGVRIKTFQGCSGYARKISFEKIVLSNARNPIIINQLFIPSIY--DTKAGAIEI 348

Query: 329 SDVTYRDVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEEAKSFCQNADGESTS 388
           SDVTY D+ GTSAN++AI L C     C NI+M  ++IT      +  + C NA G +T 
Sbjct: 349 SDVTYSDIRGTSANDQAIDLRCDNVVGCRNIVMRNIDITPGVDCPKTYTVCNNAHGTATE 408

Query: 389 TVPPVPCLS 395
           T P VPCLS
Sbjct: 409 TQPRVPCLS 410