Carg22516 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg22516
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Carbonic anhydrase
Location	Cucurbita_argyrosperma_scaffold_156 : 321777 .. 324754 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGGATTTTCTCTTTGAAATGTTAGAAAATTAGGAAAGTGGCCAAACGCAAACACGATATGATCTCTATGTCATCTCCAACGAAGTGGGGATCGATGTGGATAAGCTTGAAACGAGATGTTCATCTTTACAATTTCGCGTGTATAAATGGAACAGAAATTCAGCTGTAAGAAACTTTTCAATACAGTCGATCATTTCAGTTGGGAAAGGCGGGAACCCAAATGGGTCGTTTAATTCGTAAGTTTTCTGTACATTTTTCTTCATCCAATTGCTTGATTTCGTGCCTGTGTGCTTGTTGGGTACGTTTCAGATTCAATTTCTTTGTGAAATTATCGTTGCTGATAGGTCCAAGAGCTTGCTGGAGTTGGTTATTTTCAGAAATGGCTGCAATTAGACCGACCTTAATCCACAAGAATCCTTTCCTTGTCTCATCATTTTCAGTCGGGACAAACTCTCTTTCTGTCAGTTCTTGTATCTCTCTGTTTTTCCTTTTTAATTCTTTGTTAATTCCAGTTTTAAATAGGTTTCTTAATGGACTTGGAATTTGAATCGACAGATGAAGGAAGAAATCGACGATAATCATATGGAAGTGCCATCAAATCGCAATCACGAGTGGGTTCTTTCTGCTAATTTACTTTATGAATCTGTTAATGGAAACTCCCATGAATTTTATCTTCTGGTTCTTGATTTTTTTTTTTCTCTTTATTACAAGGCTGGAGGTCTCAAGAAACTCATTAGGGCTTGCCCAAGAACGATCATCAAGGATGGGAGTGAAAAATGTGGCTCAAGTAAAGGATGCAGATAAGGTGTTTGATGAAATGAAAATGAGATTTCTGAGTTTTAAGAAGCACAACTACTTGTAAGTAGCAGTCGATACATGATGATGAGCTAATCTTTGGATCTGTTAGGCAAGATAAAACTCAATTTGTGCTTGGTTTTTAATCCAATGATTTCATTGAAACAGGAAAAATTTAGAGCATTTTCAAGCTCTGGCAGAGCTACAAGCCCCTAAGGTAGTTAAGATGATGAACAAGTCCCTATGATTTCTCTTTGATTATTGTGTCCTGTTTGTACTATCTCTGCTCATTATCTTGAATATTTCAACACAAGCTCACAAGTAGAACAGTTCATGGTGATTTCTTGTGTCGATTCTAGAGTATGCCCATCTAACATTCTTGGATTTCAACCCGGAGAAGCTTTCACCGTTCGCAACGTAGCGAACATCGTTCCGTTATGGGAGGTATATATATAGTCTAGTGGGAATGTCTTGTATTATCTAAGTCAGACAAGCAAAGAACTAACTTCAAGTTCTTATGCAGAATGGACCAACTGAAACCAAAGCAGCCCTTGAATTTGCAGTAAATACACTTAAAGTGGGTTGTTTTCCGTTCTTCATTCAGTTGAATTTGATCTTTATAATCTCTAGACTTCATATTAAGAGAGCTATTTGTATACTGAAGGTTGAGAACATTTTAGTCATTGGCCACAGTAGCTGTGCTGGAATTCAGAGCCTTATGAGCATGGAAGATGATGCTACCGACTCGAGGTTTCACTTTCTCAAACTCCATCGTGTCTTCTTAATCTGTTTTTCTCGATTGTGCTCGTTTTCGATCTGTAATTTATAGCTTATTCTGCTATGGTAGCTTTGTTCATAAATGGATGGTGAATGCAAAAGTTGCTAAATTAAGAGCAAAAGCTGCTGCTGCACATCTTAATTTTGACCAGCAATGTAAACATTGTGAGAAGGTATTGATCTTCTTGTTGCATGAACTTTTAGATTAGAATCCATTTCTTTATTAGTTCGTATTCATAATTCCTTATTCTGTTACCCCGTTGCGTTAGTCCACAAGCTAAGACTCTATGACTGATGATGCTATATTCTCGGGCCATGGTAAAAGGCTCGAAGGGAATGAGTTCATGTCATGACAACTAATTGCTTAGAATGTTATTCTACGAGTTTTCTCAGCAAGCGAGTGTAGTGGGGTTGATATGGTATTTAGTGCAAATAGTCGAGGTGCACACAAGTTAACTCAAAAGATTACCAATCTCGGTTCCCCTTTTGCCACCGAAGAATCAATTTCCTTATTAGTTCGTATGCATAATTCCTTACTTTGTTACCCTTGGGTTAGTCCAAAAGATTTATATTGATTTTGACAAGCTAAGACTATATGCTTGATGTTATATTCTCGGGCCATAGTAAAAGGCTCGAAGGTAATGAGTTCATGTCATGAAGACTCGTTGCCTAGAATATTATTCTACGAGTTTCCTCGGAAAGTGAGCATAGTGGGGTTGATCTGTTATCTGGTGCAAATAGTCGAGGTGCATACAAGTTAACTCAAAAGATTACTAATCTCGGTTCCCATTTTGCCACTAAGAATCAATCAACTTTTATATTAGAATCCATTTCTTTATTAGTTCGTATGCATAATTCGTTACTCTATTATCCCGTTGAGTTAGTCCAAAAGCTTTATATTTCTTTCGACAAGCTAAGTCTACATGACCGATGCTATATTTTCGAGTCATAGTAAAAGGTTCGAAGGGAATGAGTTCATGTCATGATGACTCATCGCCTAGAATATTATTCTACGAGTTCCCTCAGCAAGCGAGTGTAGTGGGGTTGATATGTTAGTCAGTGCAAATAGTCGAGGTGCACACAAGTTACCTCAAAAGATTACTAATCTCAACGGTTTTCCTTTTTGCCACCAGGAATCGATCAACCTTTCATTGAAGAACTTAATGACATATCCATGGATCGAAGAGCGGTTAAAACAGGAATCGCTTGCTGTTCATGGAGGATACTATGATTTCTTGAGTTGTACATTTGAGAAGTGGACTCTTGATTACAAGAACACCTGCAAAGCTGCTGGTGATGATGATCATGGATTTCAGATCAAAAATCAATCAGTATGGTGTTGATATCATCTTTGTAAAGTTCAAAAGTCGCTGCTATATTTGTAAATGGTGACAAATATTTTTACC

mRNA sequence

GGGGATTTTCTCTTTGAAATGTTAGAAAATTAGGAAAGTGGCCAAACGCAAACACGATATGATCTCTATGTCATCTCCAACGAAGTGGGGATCGATGTGGATAAGCTTGAAACGAGATGTTCATCTTTACAATTTCGCGTGTATAAATGGAACAGAAATTCAGCTGTAAGAAACTTTTCAATACAGTCGATCATTTCAGTTGGGAAAGGCGGGAACCCAAATGGGTCGTTTAATTCGTCCAAGAGCTTGCTGGAGTTGGTTATTTTCAGAAATGGCTGCAATTAGACCGACCTTAATCCACAAGAATCCTTTCCTTGTCTCATCATTTTCAGTCGGGACAAACTCTCTTTCTATGAAGGAAGAAATCGACGATAATCATATGGAAGTGCCATCAAATCGCAATCACGAGCTGGAGGTCTCAAGAAACTCATTAGGGCTTGCCCAAGAACGATCATCAAGGATGGGAGTGAAAAATGTGGCTCAAGTAAAGGATGCAGATAAGGTGTTTGATGAAATGAAAATGAGATTTCTGAGTTTTAAGAAGCACAACTACTTGAAAAATTTAGAGCATTTTCAAGCTCTGGCAGAGCTACAAGCCCCTAAGTTCATGGTGATTTCTTGTGTCGATTCTAGAGTATGCCCATCTAACATTCTTGGATTTCAACCCGGAGAAGCTTTCACCGTTCGCAACGTAGCGAACATCGTTCCGTTATGGGAGAATGGACCAACTGAAACCAAAGCAGCCCTTGAATTTGCAGTAAATACACTTAAAGTGGGTTGTTTTCCGTTCTTCATTCAGTTGAATTTGATCTTTATAATCTCTAGACTTCATATTAAGAGAGCTATTTGTATACTGAAGGTTGAGAACATTTTAGTCATTGGCCACAGTAGCTGTGCTGGAATTCAGAGCCTTATGAGCATGGAAGATGATGCTACCGACTCGAGCTTATTCTGCTATGGTAGCTTTGTTCATAAATGGATGGTGAATGCAAAAGTTGCTAAATTAAGAGCAAAAGCTGCTGCTGCACATCTTAATTTTGACCAGCAATGTAAACATTGTGAGAAGGAATCGATCAACCTTTCATTGAAGAACTTAATGACATATCCATGGATCGAAGAGCGGTTAAAACAGGAATCGCTTGCTGTTCATGGAGGATACTATGATTTCTTGAGTTGTACATTTGAGAAGTGGACTCTTGATTACAAGAACACCTGCAAAGCTGCTGGTGATGATGATCATGGATTTCAGATCAAAAATCAATCAGTATGGTGTTGATATCATCTTTGTAAAGTTCAAAAGTCGCTGCTATATTTGTAAATGGTGACAAATATTTTTACC

Coding sequence (CDS)

ATGGGTCGTTTAATTCGTCCAAGAGCTTGCTGGAGTTGGTTATTTTCAGAAATGGCTGCAATTAGACCGACCTTAATCCACAAGAATCCTTTCCTTGTCTCATCATTTTCAGTCGGGACAAACTCTCTTTCTATGAAGGAAGAAATCGACGATAATCATATGGAAGTGCCATCAAATCGCAATCACGAGCTGGAGGTCTCAAGAAACTCATTAGGGCTTGCCCAAGAACGATCATCAAGGATGGGAGTGAAAAATGTGGCTCAAGTAAAGGATGCAGATAAGGTGTTTGATGAAATGAAAATGAGATTTCTGAGTTTTAAGAAGCACAACTACTTGAAAAATTTAGAGCATTTTCAAGCTCTGGCAGAGCTACAAGCCCCTAAGTTCATGGTGATTTCTTGTGTCGATTCTAGAGTATGCCCATCTAACATTCTTGGATTTCAACCCGGAGAAGCTTTCACCGTTCGCAACGTAGCGAACATCGTTCCGTTATGGGAGAATGGACCAACTGAAACCAAAGCAGCCCTTGAATTTGCAGTAAATACACTTAAAGTGGGTTGTTTTCCGTTCTTCATTCAGTTGAATTTGATCTTTATAATCTCTAGACTTCATATTAAGAGAGCTATTTGTATACTGAAGGTTGAGAACATTTTAGTCATTGGCCACAGTAGCTGTGCTGGAATTCAGAGCCTTATGAGCATGGAAGATGATGCTACCGACTCGAGCTTATTCTGCTATGGTAGCTTTGTTCATAAATGGATGGTGAATGCAAAAGTTGCTAAATTAAGAGCAAAAGCTGCTGCTGCACATCTTAATTTTGACCAGCAATGTAAACATTGTGAGAAGGAATCGATCAACCTTTCATTGAAGAACTTAATGACATATCCATGGATCGAAGAGCGGTTAAAACAGGAATCGCTTGCTGTTCATGGAGGATACTATGATTTCTTGAGTTGTACATTTGAGAAGTGGACTCTTGATTACAAGAACACCTGCAAAGCTGCTGGTGATGATGATCATGGATTTCAGATCAAAAATCAATCAGTATGGTGTTGA

Protein sequence

MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC

Homology

[Close]

BLAST of Carg22516 vs. NCBI nr
Match: XP_023537827.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634.0 bits (1634), Expect = 3.0e-178
Identity = 315/351 (89.74%), Postives = 318/351 (90.60%), Query Frame = 0


Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEID+NH+EVPSNR
Sbjct: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

[Close]

BLAST of Carg22516 vs. NCBI nr
Match: XP_022951348.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 633.6 bits (1633), Expect = 3.9e-178
Identity = 316/351 (90.03%), Postives = 318/351 (90.60%), Query Frame = 0


Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NH+EV SNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

[Close]

BLAST of Carg22516 vs. NCBI nr
Match: XP_023001870.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 625.5 bits (1612), Expect = 1.1e-175
Identity = 312/351 (88.89%), Postives = 316/351 (90.03%), Query Frame = 0


Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NHMEVPSNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHMEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
            HELEVSRNSLGLAQERSS MGVKNVAQVKDADKVFDEMKMRFLSFKKH YL+NLEHFQA
Sbjct: 61  YHELEVSRNSLGLAQERSSWMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLENLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             LKVENILVIGHSSCAGIQSLMSMEDDATD
Sbjct: 181 NT-----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFCYGSFVHKWM NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE
Sbjct: 241 SSLFCYGSFVHKWMANAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAG+DDHGFQIKNQS+WC
Sbjct: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGNDDHGFQIKNQSLWC 322

[Close]

BLAST of Carg22516 vs. NCBI nr
Match: XP_023537825.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.2 bits (1611), Expect = 1.4e-175
Identity = 311/348 (89.37%), Postives = 314/348 (90.23%), Query Frame = 0


Query: 4   LIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHE 63
           LI PRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEID+NH+EVPSNRNHE
Sbjct: 46  LIGPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNRNHE 105

Query: 64  LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAE 123
           LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQALAE
Sbjct: 106 LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAE 165

Query: 124 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTL 183
           LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAVNT 
Sbjct: 166 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNT- 225

Query: 184 KVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSL 243
                                       LKVENILVIGHSSCAGIQSLMSMEDDATDSSL
Sbjct: 226 ----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATDSSL 285

Query: 244 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 303
           FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK
Sbjct: 286 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 345

Query: 304 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 346 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 364

[Close]

BLAST of Carg22516 vs. NCBI nr
Match: XP_022951346.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 1.8e-175
Identity = 312/348 (89.66%), Postives = 314/348 (90.23%), Query Frame = 0


Query: 4   LIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHE 63
           LI PRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEID+NH+EV SNRNHE
Sbjct: 46  LIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNRNHE 105

Query: 64  LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAE 123
           LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKH YLKNLEHFQALAE
Sbjct: 106 LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAE 165

Query: 124 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTL 183
           LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVP WENGPTET AALEFAVNT 
Sbjct: 166 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNT- 225

Query: 184 KVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSL 243
                                       LKVENILVIGHSSCAGIQSLMSMEDDATDSSL
Sbjct: 226 ----------------------------LKVENILVIGHSSCAGIQSLMSMEDDATDSSL 285

Query: 244 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 303
           FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK
Sbjct: 286 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 345

Query: 304 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 346 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 364

[Close]

BLAST of Carg22516 vs. TAIR10
Match: AT4G33580.2 (beta carbonic anhydrase 5)

HSP 1 Score: 275.0 bits (702), Expect = 6.3e-74
Identity = 148/291 (50.86%), Postives = 195/291 (67.01%), Query Frame = 0


Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLK-NLEHFQ 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK  Y++ + EH++
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYIRDDFEHYK 111

Query: 121 ALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFA 180
            LA+ QAPKF+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +E+GPTETKAALEF+
Sbjct: 112 NLADAQAPKFLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPYESGPTETKAALEFS 171

Query: 181 VNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDAT 240
           VNT                             L VENILVIGHS C GIQ+LM MED+  
Sbjct: 172 VNT-----------------------------LNVENILVIGHSRCGGIQALMKMEDEG- 231

Query: 241 DSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIE 300
           DS      SF+H W+V  K AK   KA A++L+FD QC+HCEK SIN SL+ L+ YPWIE
Sbjct: 232 DSR-----SFIHNWVVVGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIE 291

Query: 301 ERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
           E+++Q SL++HGGYY+F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 EKVRQGSLSLHGGYYNFVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 301

[Close]

BLAST of Carg22516 vs. TAIR10
Match: AT1G58180.2 (beta carbonic anhydrase 6)

HSP 1 Score: 234.2 bits (596), Expect = 1.2e-61
Identity = 130/330 (39.39%), Postives = 185/330 (56.06%), Query Frame = 0


Query: 21  IRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      +   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVC 140
                    + +    DEM+ RFL FK+  YL  +E F+ALA  Q+PK MVI C DSRVC
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKVMVIGCADSRVC 132

Query: 141 PSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFII 200
           PS +LGFQPGEAFT+RNVAN+V   +NGPTET +ALEFAV T                  
Sbjct: 133 PSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTT------------------ 192

Query: 201 SRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVA 260
                      L+VENI+V+GHS+C GI +LMS ++     S     S V +W++N K A
Sbjct: 193 -----------LQVENIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAA 252

Query: 261 KLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCT 320
           KLR + A++HL+FD+QC++CEKESI  S+ NL+TY WI +R+K+  + +HG YY+   C+
Sbjct: 253 KLRTQLASSHLSFDEQCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCS 289

Query: 321 FEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
            EKW L    T       ++GF I ++ +W
Sbjct: 313 LEKWRLSSDKT-------NYGFYISDREIW 289

[Close]

BLAST of Carg22516 vs. TAIR10
Match: AT1G70410.2 (beta carbonic anhydrase 4)

HSP 1 Score: 187.2 bits (474), Expect = 1.7e-47
Identity = 103/250 (41.20%), Postives = 145/250 (58.00%), Query Frame = 0


Query: 87  AQVKDADK----VFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPS 146
           A++K+ D       + +K  F  FK   YLKN   F  LA+ Q PKF+V +C DSRVCPS
Sbjct: 58  AELKELDSSNSDAIERIKTGFTQFKTEKYLKNSTLFNHLAKTQTPKFLVFACSDSRVCPS 117

Query: 147 NILGFQPGEAFTVRNVANIVPLW-ENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFIIS 206
           +IL FQPGEAF VRN+AN+VP + +   +   AA+E+AV                     
Sbjct: 118 HILNFQPGEAFVVRNIANMVPPFDQKRHSGVGAAVEYAV--------------------- 177

Query: 207 RLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAK 266
            +H       LKVENILVIGHS C GI+ LMS+EDDA  +       F+  W+     A+
Sbjct: 178 -VH-------LKVENILVIGHSCCGGIKGLMSIEDDAAPTQ----SDFIENWVKIGASAR 237

Query: 267 LRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTF 326
            + K     L++D QC  CEKE++N+SL NL++YP++   + + +LA+ GG+Y+F+  TF
Sbjct: 238 NKIKEEHKDLSYDDQCNKCEKEAVNVSLGNLLSYPFVRAEVVKNTLAIRGGHYNFVKGTF 274

Query: 327 EKWTLDYKNT 332
           + W LD+K T
Sbjct: 298 DLWELDFKTT 274

[Close]

BLAST of Carg22516 vs. TAIR10
Match: AT1G23730.1 (beta carbonic anhydrase 3)

HSP 1 Score: 179.5 bits (454), Expect = 3.6e-45
Identity = 108/286 (37.76%), Postives = 158/286 (55.24%), Query Frame = 0


Query: 58  SNRNHELEVSRNSLGLAQERSSRMGVKNVAQVK---------DADKV--FDEMKMRFLSF 117
           S  ++E  + R  LG    + S +G    A++K         D++K+   + +K  FL F
Sbjct: 2   STESYEDAIKR--LGELLSKKSDLGNVAAAKIKKLTDELEELDSNKLDAVERIKSGFLHF 61

Query: 118 KKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWE 177
           K +NY KN   + +LA+ Q PKF+V +C DSRV PS+IL FQ GEAF VRN+AN+VP ++
Sbjct: 62  KTNNYEKNPTLYNSLAKSQTPKFLVFACADSRVSPSHILNFQLGEAFIVRNIANMVPPYD 121

Query: 178 NGP-TETKAALEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSC 237
               +   AALE+                              I +L VENILVIGHS C
Sbjct: 122 KTKHSNVGAALEY-----------------------------PITVLNVENILVIGHSCC 181

Query: 238 AGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESI 297
            GI+ LM++ED+   +       F+  W+     AK R K     L+F+ QC +CEKE++
Sbjct: 182 GGIKGLMAIEDNTAPTKT----EFIENWIQICAPAKNRIKQDCKDLSFEDQCTNCEKEAV 241

Query: 298 NLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNT 332
           N+SL NL++YP++ ER+ +  LA+ G +YDF+  TF+ W LD+K T
Sbjct: 242 NVSLGNLLSYPFVRERVVKNKLAIRGAHYDFVKGTFDLWELDFKTT 252

[Close]

BLAST of Carg22516 vs. TAIR10
Match: AT5G14740.1 (carbonic anhydrase 2)

HSP 1 Score: 167.2 bits (422), Expect = 1.9e-41
Identity = 93/233 (39.91%), Postives = 136/233 (58.37%), Query Frame = 0


Query: 97  DEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVR 156
           + +K  F++FKK  Y  N   +  LA+ Q+PK+MV +C DSRVCPS++L F PG+AF VR
Sbjct: 125 ERIKEGFVTFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFHPGDAFVVR 184

Query: 157 NVANIVPLWENGP-TETKAALEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVE 216
           N+AN+VP ++        AA+E+AV                      LH       LKVE
Sbjct: 185 NIANMVPPFDKVKYAGVGAAIEYAV----------------------LH-------LKVE 244

Query: 217 NILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQ 276
           NI+VIGHS+C GI+ LMS   D  +S+      F+  W+     AK +  A +    F+ 
Sbjct: 245 NIVVIGHSACGGIKGLMSFPLDGNNST-----DFIEDWVKICLPAKSKVLAESESSAFED 304

Query: 277 QCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDY 329
           QC  CE+E++N+SL NL+TYP++ E + + +LA+ GGYYDF++ +FE W L +
Sbjct: 305 QCGRCEREAVNVSLANLLTYPFVREGVVKGTLALKGGYYDFVNGSFELWELQF 323

[Close]

BLAST of Carg22516 vs. Swiss-Prot
Match: sp|Q94CE3|BCA5_ARATH (Beta carbonic anhydrase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA5 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.0e-73
Identity = 148/290 (51.03%), Postives = 194/290 (66.90%), Query Frame = 0


Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK  Y+ + EH++ 
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYMDDFEHYKN 111

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LA+ QAPKF+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +E+GPTETKAALEF+V
Sbjct: 112 LADAQAPKFLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPYESGPTETKAALEFSV 171

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             L VENILVIGHS C GIQ+LM MED+  D
Sbjct: 172 NT-----------------------------LNVENILVIGHSRCGGIQALMKMEDEG-D 231

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           S      SF+H W+V  K AK   KA A++L+FD QC+HCEK SIN SL+ L+ YPWIEE
Sbjct: 232 SR-----SFIHNWVVVGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIEE 291

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
           +++Q SL++HGGYY+F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 KVRQGSLSLHGGYYNFVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 300

[Close]

BLAST of Carg22516 vs. Swiss-Prot
Match: sp|Q9C6F5|BCA6_ARATH (Beta carbonic anhydrase 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCA6 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-60
Identity = 130/330 (39.39%), Postives = 185/330 (56.06%), Query Frame = 0


Query: 21  IRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      +   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVC 140
                    + +    DEM+ RFL FK+  YL  +E F+ALA  Q+PK MVI C DSRVC
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKVMVIGCADSRVC 132

Query: 141 PSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFII 200
           PS +LGFQPGEAFT+RNVAN+V   +NGPTET +ALEFAV T                  
Sbjct: 133 PSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTT------------------ 192

Query: 201 SRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVA 260
                      L+VENI+V+GHS+C GI +LMS ++     S     S V +W++N K A
Sbjct: 193 -----------LQVENIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAA 252

Query: 261 KLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCT 320
           KLR + A++HL+FD+QC++CEKESI  S+ NL+TY WI +R+K+  + +HG YY+   C+
Sbjct: 253 KLRTQLASSHLSFDEQCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCS 289

Query: 321 FEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
            EKW L    T       ++GF I ++ +W
Sbjct: 313 LEKWRLSSDKT-------NYGFYISDREIW 289

[Close]

BLAST of Carg22516 vs. Swiss-Prot
Match: sp|Q94CE4|BCA4_ARATH (Beta carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=BCA4 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.1e-46
Identity = 103/250 (41.20%), Postives = 145/250 (58.00%), Query Frame = 0


Query: 87  AQVKDADK----VFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPS 146
           A++K+ D       + +K  F  FK   YLKN   F  LA+ Q PKF+V +C DSRVCPS
Sbjct: 58  AELKELDSSNSDAIERIKTGFTQFKTEKYLKNSTLFNHLAKTQTPKFLVFACSDSRVCPS 117

Query: 147 NILGFQPGEAFTVRNVANIVPLW-ENGPTETKAALEFAVNTLKVGCFPFFIQLNLIFIIS 206
           +IL FQPGEAF VRN+AN+VP + +   +   AA+E+AV                     
Sbjct: 118 HILNFQPGEAFVVRNIANMVPPFDQKRHSGVGAAVEYAV--------------------- 177

Query: 207 RLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAK 266
            +H       LKVENILVIGHS C GI+ LMS+EDDA  +       F+  W+     A+
Sbjct: 178 -VH-------LKVENILVIGHSCCGGIKGLMSIEDDAAPTQ----SDFIENWVKIGASAR 237

Query: 267 LRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTF 326
            + K     L++D QC  CEKE++N+SL NL++YP++   + + +LA+ GG+Y+F+  TF
Sbjct: 238 NKIKEEHKDLSYDDQCNKCEKEAVNVSLGNLLSYPFVRAEVVKNTLAIRGGHYNFVKGTF 274

Query: 327 EKWTLDYKNT 332
           + W LD+K T
Sbjct: 298 DLWELDFKTT 274

[Close]

BLAST of Carg22516 vs. Swiss-Prot
Match: sp|P17067|CAHC_PEA (Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.0e-45
Identity = 114/309 (36.89%), Postives = 166/309 (53.72%), Query Frame = 0


Query: 23  PTLIHKNPFLVSSFSVGTNSL--SMKEEIDDNHMEVPSNRNHELEVSRNSLGLAQERSSR 82
           P+LI   P   SS  + T  L   M +  D+   E+      + E+   +    ++ +++
Sbjct: 46  PSLIQDKPVFASSSPIITPVLREEMGKGYDEAIEELQKLLREKTELKATAAEKVEQITAQ 105

Query: 83  MGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAELQAPKFMVISCVDSRVC 142
           +G  + +      +  + +K  FL FKK  Y KN   +  LA+ Q+P FMV +C DSRVC
Sbjct: 106 LGTTSSSDGIPKSEASERIKTGFLHFKKEKYDKNPALYGELAKGQSPPFMVFACSDSRVC 165

Query: 143 PSNILGFQPGEAFTVRNVANIVPLWENGP-TETKAALEFAVNTLKVGCFPFFIQLNLIFI 202
           PS++L FQPGEAF VRNVAN+VP ++      T AA+E+AV                   
Sbjct: 166 PSHVLDFQPGEAFVVRNVANLVPPYDQAKYAGTGAAIEYAV------------------- 225

Query: 203 ISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKV 262
              LH       LKV NI+VIGHS+C GI+ L+S   D T S+      F+ +W+     
Sbjct: 226 ---LH-------LKVSNIVVIGHSACGGIKGLLSFPFDGTYST-----DFIEEWVKIGLP 285

Query: 263 AKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSC 322
           AK + KA      F + C HCEKE++N SL NL+TYP++ E L  ++LA+ GGYYDF+  
Sbjct: 286 AKAKVKAQHGDAPFAELCTHCEKEAVNASLGNLLTYPFVREGLVNKTLALKGGYYDFVKG 320

Query: 323 TFEKWTLDY 329
           +FE W L++
Sbjct: 346 SFELWGLEF 320

[Close]

BLAST of Carg22516 vs. Swiss-Prot
Match: sp|Q9ZUC2|BCA3_ARATH (Beta carbonic anhydrase 3 OS=Arabidopsis thaliana OX=3702 GN=BCA3 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.5e-44
Identity = 108/286 (37.76%), Postives = 158/286 (55.24%), Query Frame = 0


Query: 58  SNRNHELEVSRNSLGLAQERSSRMGVKNVAQVK---------DADKV--FDEMKMRFLSF 117
           S  ++E  + R  LG    + S +G    A++K         D++K+   + +K  FL F
Sbjct: 2   STESYEDAIKR--LGELLSKKSDLGNVAAAKIKKLTDELEELDSNKLDAVERIKSGFLHF 61

Query: 118 KKHNYLKNLEHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWE 177
           K +NY KN   + +LA+ Q PKF+V +C DSRV PS+IL FQ GEAF VRN+AN+VP ++
Sbjct: 62  KTNNYEKNPTLYNSLAKSQTPKFLVFACADSRVSPSHILNFQLGEAFIVRNIANMVPPYD 121

Query: 178 NGP-TETKAALEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSC 237
               +   AALE+                              I +L VENILVIGHS C
Sbjct: 122 KTKHSNVGAALEY-----------------------------PITVLNVENILVIGHSCC 181

Query: 238 AGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESI 297
            GI+ LM++ED+   +       F+  W+     AK R K     L+F+ QC +CEKE++
Sbjct: 182 GGIKGLMAIEDNTAPTKT----EFIENWIQICAPAKNRIKQDCKDLSFEDQCTNCEKEAV 241

Query: 298 NLSLKNLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNT 332
           N+SL NL++YP++ ER+ +  LA+ G +YDF+  TF+ W LD+K T
Sbjct: 242 NVSLGNLLSYPFVRERVVKNKLAIRGAHYDFVKGTFDLWELDFKTT 252

[Close]

BLAST of Carg22516 vs. TrEMBL
Match: tr|A0A1S3BCA0|A0A1S3BCA0_CUCME (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103488130 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 4.6e-135
Identity = 250/352 (71.02%), Postives = 275/352 (78.12%), Query Frame = 0


Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGR IRPRA W  L SEM AIRPTLIHKNPFL SSFSVG +SLS+KEE D+  +EVPSNR
Sbjct: 1   MGRSIRPRAFWGLLISEMVAIRPTLIHKNPFLFSSFSVGRSSLSVKEETDETQLEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
            HELE                 VKNVA+ KD  K+FDEM+ RFLSFKKH YL++LEHFQA
Sbjct: 61  IHELE-----------------VKNVAKSKDEHKLFDEMRRRFLSFKKHKYLEHLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAF VRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFMVRNVANIVPPWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             L+VENILVIGHSSCAGIQSLMSM+D+ATD
Sbjct: 181 NT-----------------------------LEVENILVIGHSSCAGIQSLMSMQDNATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           SSLFC+GSFVHKW+VNAK AKLRAKAAAAHL+FDQQCKHCEKESINLSLKNLM+YPWIEE
Sbjct: 241 SSLFCHGSFVHKWVVNAKAAKLRAKAAAAHLSFDQQCKHCEKESINLSLKNLMSYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGF-QIKNQSVWC 352
           RLKQ+ ++VHGGYYDFL+CTFEKW+LDYKNT +   DDD     IK+Q++WC
Sbjct: 301 RLKQDLISVHGGYYDFLNCTFEKWSLDYKNTSRVDNDDDDRLCHIKDQTIWC 306

[Close]

BLAST of Carg22516 vs. TrEMBL
Match: tr|A0A1S3BCE3|A0A1S3BCE3_CUCME (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103488130 PE=3 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 1.7e-129
Identity = 245/352 (69.60%), Postives = 269/352 (76.42%), Query Frame = 0


Query: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDDNHMEVPSNR 60
           MGR IRPRA W  L SEM AIRPTLIHKNPFL SSFSVG +SLS+KEE D+  +EVPSNR
Sbjct: 1   MGRSIRPRAFWGLLISEMVAIRPTLIHKNPFLFSSFSVGRSSLSVKEETDETQLEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQA 120
            HELE                 VKNVA+ KD  K+FDEM+ RFLSFKKH YL++LEHFQA
Sbjct: 61  IHELE-----------------VKNVAKSKDEHKLFDEMRRRFLSFKKHKYLEHLEHFQA 120

Query: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAV 180
           LAELQAPKFMVISCVDSRVCPSNILGFQPGEAF VRNVANIVP WENGPTET AALEFAV
Sbjct: 121 LAELQAPKFMVISCVDSRVCPSNILGFQPGEAFMVRNVANIVPPWENGPTETNAALEFAV 180

Query: 181 NTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATD 240
           NT                             L+VENILVIGHSSCAGIQSLMSM+D+ATD
Sbjct: 181 NT-----------------------------LEVENILVIGHSSCAGIQSLMSMQDNATD 240

Query: 241 SSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEE 300
           S      SFVHKW+VNAK AKLRAKAAAAHL+FDQQCKHCEKESINLSLKNLM+YPWIEE
Sbjct: 241 S------SFVHKWVVNAKAAKLRAKAAAAHLSFDQQCKHCEKESINLSLKNLMSYPWIEE 300

Query: 301 RLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGF-QIKNQSVWC 352
           RLKQ+ ++VHGGYYDFL+CTFEKW+LDYKNT +   DDD     IK+Q++WC
Sbjct: 301 RLKQDLISVHGGYYDFLNCTFEKWSLDYKNTSRVDNDDDDRLCHIKDQTIWC 300

[Close]

BLAST of Carg22516 vs. TrEMBL
Match: tr|A0A2P5E1C7|A0A2P5E1C7_9ROSA (Carbonic anhydrase OS=Trema orientalis OX=63057 GN=TorBCA6 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 9.7e-85
Identity = 170/355 (47.89%), Postives = 228/355 (64.23%), Query Frame = 0


Query: 13  WLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKE-------------EIDDNHM----E 72
           W+   +AA+RP+ ++++PF    FS   NS   +              ++++ H+     
Sbjct: 29  WIIPSLAALRPSTVYRDPFHCIPFSGLKNSFLGRPSGSDPRITQINLVQVEETHLRLLPS 88

Query: 73  VPSNRNHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNL 132
           V  N N  LE S +   LA++  +   V+NV +  +   +FDEM+ RFLSFKK  YL+NL
Sbjct: 89  VKRNPNFGLEASSDYSALAKDHHTNFIVRNVPKANNEVDLFDEMRHRFLSFKKRIYLENL 148

Query: 133 EHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAA 192
           EH+Q LAELQ PKFMVI+C DSRVCPSNILGFQPGEAF +RNVAN+VP  ENGP+ET AA
Sbjct: 149 EHYQRLAELQTPKFMVIACADSRVCPSNILGFQPGEAFMIRNVANLVPPLENGPSETNAA 208

Query: 193 LEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSME 252
           LEFAVNT                             L+VENILVIGHS C GI++LMSM+
Sbjct: 209 LEFAVNT-----------------------------LEVENILVIGHSRCGGIETLMSMQ 268

Query: 253 DDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTY 312
           ++          SF++KW++ AKVA+LR +AAA   +FDQQC+HCEKESIN SL NL+TY
Sbjct: 269 EELN-------SSFIYKWIIKAKVARLRTRAAALDHSFDQQCRHCEKESINDSLLNLLTY 328

Query: 313 PWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
           PWIE+R+K+  L++HGGYYDFL+CTFEKWTL+     + +  D+  + +K+Q+ W
Sbjct: 329 PWIEDRVKKNQLSIHGGYYDFLNCTFEKWTLEIN---EDSSIDEGRYLVKDQAFW 344

[Close]

BLAST of Carg22516 vs. TrEMBL
Match: tr|A0A2I4HQX8|A0A2I4HQX8_9ROSI (Carbonic anhydrase OS=Juglans regia OX=51240 GN=LOC109020529 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.3e-84
Identity = 164/288 (56.94%), Postives = 202/288 (70.14%), Query Frame = 0


Query: 64  LEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNLEHFQALAE 123
           L+ + +SLG AQE  +   +K+V ++     +F EMK RFL+FKK  Y+K  E+FQ +AE
Sbjct: 88  LKAASSSLGSAQEHKN-YNLKDVGEIDGGQDLFVEMKQRFLNFKKQKYIKESEYFQTIAE 147

Query: 124 LQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAALEFAVNTL 183
            Q PKFMVI+C DSRVCPSN+LGFQPGEAF +RNVAN+VP  ENGP+ET AALEFAVNT 
Sbjct: 148 AQYPKFMVIACADSRVCPSNVLGFQPGEAFMIRNVANLVPPLENGPSETNAALEFAVNT- 207

Query: 184 KVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSMEDDATDSSL 243
                                       L+VENILVIGHSSC GIQ+LMSM+DDA DS  
Sbjct: 208 ----------------------------LEVENILVIGHSSCGGIQALMSMQDDA-DSK- 267

Query: 244 FCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLK 303
               SF+HKW+VNAK AKLRA+A AAH +FDQQC+HCEK+S+N SL NL+TYPWIE+R +
Sbjct: 268 ----SFLHKWVVNAKAAKLRAEADAAHYSFDQQCRHCEKDSVNRSLMNLLTYPWIEDRFR 327

Query: 304 QESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 352
           +E L +HGGYYDFL+CTFEKWTLD K +    G     F +K+Q+ WC
Sbjct: 328 KELLCIHGGYYDFLNCTFEKWTLDLKGSSIEEGG---AFSVKDQAFWC 336

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BLAST of Carg22516 vs. TrEMBL
Match: tr|A0A2P5CR37|A0A2P5CR37_PARAD (Carbonic anhydrase OS=Parasponia andersonii OX=3476 GN=PanBCA6 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.8e-84
Identity = 170/355 (47.89%), Postives = 227/355 (63.94%), Query Frame = 0


Query: 13  WLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKE-------------EIDDNHM----E 72
           W+   +AA+RP+ ++++PF    FS  TNS   +              ++++ H+     
Sbjct: 29  WIIPSLAALRPSTVYRDPFRCIPFSRLTNSFLGRPSRSDPRIRQINLVQVEETHLRLLPS 88

Query: 73  VPSNRNHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHNYLKNL 132
           V  N N  LE S +   LA++  +   V+NV +  +   +FDEM+ RFLSFKK  YL+NL
Sbjct: 89  VKRNPNFGLEASSDDSALAKDHHTNFIVRNVPKANNEVDLFDEMRQRFLSFKKRIYLENL 148

Query: 133 EHFQALAELQAPKFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPLWENGPTETKAA 192
           EH+Q LAE Q PKFMVI+C DSRVCPSNILGFQPGEAF +RNVAN+VP  ENGP+ET AA
Sbjct: 149 EHYQRLAEEQTPKFMVIACADSRVCPSNILGFQPGEAFMIRNVANLVPPLENGPSETNAA 208

Query: 193 LEFAVNTLKVGCFPFFIQLNLIFIISRLHIKRAICILKVENILVIGHSSCAGIQSLMSME 252
           LEFAVNT                             L+VENILVIGHS C GI++LMSM+
Sbjct: 209 LEFAVNT-----------------------------LEVENILVIGHSRCGGIETLMSMQ 268

Query: 253 DDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTY 312
           +++         SF++KW++ AKVA+LR +AAA   +FDQQC+HCEKESIN SL NL+TY
Sbjct: 269 EESN-------SSFIYKWIIKAKVARLRTRAAALDHSFDQQCRHCEKESINDSLLNLLTY 328

Query: 313 PWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 351
           PWIE+R+K+  L++HGGYYDFL+ TFEKWTLD     + +  D   + +K+Q+ W
Sbjct: 329 PWIEDRVKKNQLSIHGGYYDFLNFTFEKWTLDIN---EDSSIDGGRYLVKDQAFW 344

The following BLAST results are available for this feature:

BLAST of Carg22516 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537827.1	3.0e-178	89.74	beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita pepo subsp. ...	[more]
XP_022951348.1	3.9e-178	90.03	beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita moschata]	[more]
XP_023001870.1	1.1e-175	88.89	beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita maxima]	[more]
XP_023537825.1	1.4e-175	89.37	beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita pepo subsp. ...	[more]
XP_022951346.1	1.8e-175	89.66	beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg22516 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G33580.2	6.3e-74	50.86	beta carbonic anhydrase 5	[more]
AT1G58180.2	1.2e-61	39.39	beta carbonic anhydrase 6	[more]
AT1G70410.2	1.7e-47	41.20	beta carbonic anhydrase 4	[more]
AT1G23730.1	3.6e-45	37.76	beta carbonic anhydrase 3	[more]
AT5G14740.1	1.9e-41	39.91	carbonic anhydrase 2	[more]

BLAST of Carg22516 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q94CE3\|BCA5_ARATH	1.0e-73	51.03	Beta carbonic anhydrase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA5...	[more]
sp\|Q9C6F5\|BCA6_ARATH	2.2e-60	39.39	Beta carbonic anhydrase 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCA6...	[more]
sp\|Q94CE4\|BCA4_ARATH	3.1e-46	41.20	Beta carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=BCA4 PE=1 SV=1	[more]
sp\|P17067\|CAHC_PEA	2.0e-45	36.89	Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1	[more]
sp\|Q9ZUC2\|BCA3_ARATH	6.5e-44	37.76	Beta carbonic anhydrase 3 OS=Arabidopsis thaliana OX=3702 GN=BCA3 PE=2 SV=1	[more]

BLAST of Carg22516 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BCA0\|A0A1S3BCA0_CUCME	4.6e-135	71.02	Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103488130 PE=3 SV=1	[more]
tr\|A0A1S3BCE3\|A0A1S3BCE3_CUCME	1.7e-129	69.60	Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103488130 PE=3 SV=1	[more]
tr\|A0A2P5E1C7\|A0A2P5E1C7_9ROSA	9.7e-85	47.89	Carbonic anhydrase OS=Trema orientalis OX=63057 GN=TorBCA6 PE=3 SV=1	[more]
tr\|A0A2I4HQX8\|A0A2I4HQX8_9ROSI	1.3e-84	56.94	Carbonic anhydrase OS=Juglans regia OX=51240 GN=LOC109020529 PE=3 SV=1	[more]
tr\|A0A2P5CR37\|A0A2P5CR37_PARAD	2.8e-84	47.89	Carbonic anhydrase OS=Parasponia andersonii OX=3476 GN=PanBCA6 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0004089	carbonate dehydratase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036874	Carbonic_anhydrase_sf
IPR001765	Carbonic_anhydrase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0006730	one-carbon metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22516-RA	Carg22516-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001765	Carbonic anhydrase	SMART	SM00947	Pro_CA_2	coord: 122..325 e-value: 3.5E-33 score: 126.2
IPR001765	Carbonic anhydrase	PFAM	PF00484	Pro_CA	coord: 130..185 e-value: 4.7E-15 score: 56.1 coord: 205..316 e-value: 3.0E-17 score: 63.2
IPR036874	Carbonic anhydrase superfamily	GENE3D	G3DSA:3.40.1050.10		coord: 201..335 e-value: 7.6E-31 score: 109.1 coord: 84..187 e-value: 1.3E-29 score: 105.1
IPR036874	Carbonic anhydrase superfamily	SUPERFAMILY	SSF53056	beta-carbonic anhydrase, cab	coord: 89..185
IPR036874	Carbonic anhydrase superfamily	SUPERFAMILY	SSF53056	beta-carbonic anhydrase, cab	coord: 205..329
None	No IPR available	PANTHER	PTHR11002:SF12	BETA CARBONIC ANHYDRASE 5, CHLOROPLASTIC	coord: 46..186
None	No IPR available	PANTHER	PTHR11002	CARBONIC ANHYDRASE	coord: 208..349
None	No IPR available	PANTHER	PTHR11002:SF12	BETA CARBONIC ANHYDRASE 5, CHLOROPLASTIC	coord: 208..349
None	No IPR available	PANTHER	PTHR11002	CARBONIC ANHYDRASE	coord: 46..186

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg22516	Cla022161	Watermelon (97103) v1	carwmB0818
Carg22516	MELO3C011095	Melon (DHL92) v3.5.1	carmeB0816
Carg22516	ClCG08G011710	Watermelon (Charleston Gray)	carwcgB0795
Carg22516	CSPI02G21680	Wild cucumber (PI 183967)	carcpiB0857
Carg22516	Cucsa.160660	Cucumber (Gy14) v1	carcgyB0937
Carg22516	CmaCh05G005490	Cucurbita maxima (Rimu)	carcmaB1141
Carg22516	CmaCh12G001970	Cucurbita maxima (Rimu)	carcmaB1140
Carg22516	CmoCh12G001500	Cucurbita moschata (Rifu)	carcmoB1111
Carg22516	Lsi08G010280	Bottle gourd (USVL1VR-Ls)	carlsiB751
Carg22516	Cp4.1LG11g04900	Cucurbita pepo (Zucchini)	carcpeB1151
Carg22516	Cp4.1LG07g01640	Cucurbita pepo (Zucchini)	carcpeB1153
Carg22516	CsGy2G021400	Cucumber (Gy14) v2	carcgybB0810
Carg22516	MELO3C011095.2	Melon (DHL92) v3.6.1	carmedB0827
Carg22516	CsaV3_2G030320	Cucumber (Chinese Long) v3	carcucB0860
Carg22516	Cla97C08G154780	Watermelon (97103) v2	carwmbB0855
Carg22516	Bhi04G000767	Wax gourd	carwgoB0959

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg22516	Carg08239	Silver-seed gourd	carcarB069

The following block(s) are covering this gene:


Gene	Organism	Block
Carg22516	Silver-seed gourd	carcarB557
Carg22516	Cucumber (Gy14) v2	carcgybB0812
Carg22516	Cucumber (Gy14) v1	carcgyB0939
Carg22516	Cucurbita maxima (Rimu)	carcmaB1139
Carg22516	Cucurbita moschata (Rifu)	carcmoB1110
Carg22516	Cucurbita moschata (Rifu)	carcmoB1112
Carg22516	Wild cucumber (PI 183967)	carcpiB0859
Carg22516	Cucumber (Chinese Long) v2	carcuB0835
Carg22516	Cucumber (Chinese Long) v2	carcuB0837
Carg22516	Cucumber (Chinese Long) v3	carcucB0862
Carg22516	Bottle gourd (USVL1VR-Ls)	carlsiB750
Carg22516	Melon (DHL92) v3.5.1	carmeB0817
Carg22516	Melon (DHL92) v3.6.1	carmedB0828
Carg22516	Watermelon (Charleston Gray)	carwcgB0794
Carg22516	Watermelon (97103) v1	carwmB0821
Carg22516	Watermelon (97103) v2	carwmbB0854
Carg22516	Wax gourd	carwgoB0960