Carg22132 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg22132
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Heavy metal-associated domain protein
Location	Cucurbita_argyrosperma_scaffold_229 : 234049 .. 234282 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATGAAGGAAAAGAAAATGACGGTGATCGGGGAAGTTGATCCAATATGCATGGTGCGAAAACTAAGAAGGTTTCGTCAAACAGAAATAATCTCAGTGGGGCCACCGAAAGAAGAGGAGAAAAAGAAGGCCGAAGATAAAATTGAAGAGGTTCTCAACTACTACAGATATAATGTTCCATTTTATTCGGTTCAACCATATGAACAGCCTCCTACTTGTGTTATTTGTTAG

mRNA sequence

ATGGAAATGAAGGAAAAGAAAATGACGGTGATCGGGGAAGTTGATCCAATATGCATGGTGCGAAAACTAAGAAGGTTTCGTCAAACAGAAATAATCTCAGTGGGGCCACCGAAAGAAGAGGAGAAAAAGAAGGCCGAAGATAAAATTGAAGAGGTTCTCAACTACTACAGATATAATGTTCCATTTTATTCGGTTCAACCATATGAACAGCCTCCTACTTGTGTTATTTGTTAG

Coding sequence (CDS)

ATGGAAATGAAGGAAAAGAAAATGACGGTGATCGGGGAAGTTGATCCAATATGCATGGTGCGAAAACTAAGAAGGTTTCGTCAAACAGAAATAATCTCAGTGGGGCCACCGAAAGAAGAGGAGAAAAAGAAGGCCGAAGATAAAATTGAAGAGGTTCTCAACTACTACAGATATAATGTTCCATTTTATTCGGTTCAACCATATGAACAGCCTCCTACTTGTGTTATTTGTTAG

Protein sequence

MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNYYRYNVPFYSVQPYEQPPTCVIC

Homology

[Close]

BLAST of Carg22132 vs. NCBI nr
Match: XP_022945780.1 (heavy metal-associated isoprenylated plant protein 39-like [Cucurbita moschata])

HSP 1 Score: 158.7 bits (400), Expect = 8.0e-36
Identity = 77/77 (100.00%), Postives = 77/77 (100.00%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNYYRYNV 60
           MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNYYRYNV
Sbjct: 36  MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNYYRYNV 95

Query: 61  PFYSVQPYEQPPTCVIC 78
           PFYSVQPYEQPPTCVIC
Sbjct: 96  PFYSVQPYEQPPTCVIC 112

[Close]

BLAST of Carg22132 vs. NCBI nr
Match: XP_016201007.1 (heavy metal-associated isoprenylated plant protein 39 [Arachis ipaensis] >XP_025656929.1 heavy metal-associated isoprenylated plant protein 39-like [Arachis hypogaea])

HSP 1 Score: 70.9 bits (172), Expect = 2.2e-09
Identity = 38/83 (45.78%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAED----KIEEV--LN 60
           M+M EKK+T+ G++DP+ +  KLR+   TEI+SVGP KEEEKK + +    K+ E   L+
Sbjct: 34  MDMAEKKLTLTGDIDPVHVAGKLRKCCHTEIVSVGPAKEEEKKASSEEPAIKVFETYPLH 93

Query: 61  YYRYNVPFYSVQPYEQPPTCVIC 78
           YY+   P+Y     E P +CVIC
Sbjct: 94  YYQARAPYYVSSMEENPNSCVIC 116

[Close]

BLAST of Carg22132 vs. NCBI nr
Match: XP_015967352.1 (heavy metal-associated isoprenylated plant protein 39 [Arachis duranensis])

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-09
Identity = 37/83 (44.58%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAED----KIEEV--LN 60
           M+M EKK+T+ G++DP+ +  KLR+   T+I+SVGP KEEEKK + +    K+ E   L+
Sbjct: 34  MDMAEKKLTLTGDIDPVHVAGKLRKCCHTQIVSVGPAKEEEKKASSEETAIKVFETYPLH 93

Query: 61  YYRYNVPFYSVQPYEQPPTCVIC 78
           YY+   P+Y     E P +CVIC
Sbjct: 94  YYQARAPYYVTSMEENPNSCVIC 116

[Close]

BLAST of Carg22132 vs. NCBI nr
Match: XP_010671618.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 39 [Beta vulgaris subsp. vulgaris] >KMT16170.1 hypothetical protein BVRB_3g053310 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-08
Identity = 39/81 (48.15%), Postives = 56/81 (69.14%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEK-KKAEDKIE--EVLNY-Y 60
           ++MKE+K+TVIG++DP+ +V+KLR+   TE++SVGP KE EK K   D +   +  NY Y
Sbjct: 35  VDMKEQKLTVIGDMDPMAVVKKLRKNFYTELVSVGPAKEPEKPKSVADFVNTMQAYNYSY 94

Query: 61  RYNVPFYSVQPYEQPPTCVIC 78
            YN P+Y V+  + P  CVIC
Sbjct: 95  GYNPPYYYVE--QNPNGCVIC 113

[Close]

BLAST of Carg22132 vs. NCBI nr
Match: GAV76128.1 (HMA domain-containing protein [Cephalotus follicularis])

HSP 1 Score: 67.4 bits (163), Expect = 2.4e-08
Identity = 39/91 (42.86%), Postives = 55/91 (60.44%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEE----------KKKAEDKIE 60
           ++MKEKK+TVIG+ DP+ +V KLR+  QTEI++VGP KEE+               ++I 
Sbjct: 34  IDMKEKKLTVIGDADPVNIVEKLRKQWQTEILTVGPAKEEKXXXXXXXXXXXXXXNEQIS 93

Query: 61  EVLNYYR----YNVPFYSVQPYEQPPTCVIC 78
           E+ N Y+    Y   +Y+V   E P TCVIC
Sbjct: 94  ELANAYKAYNPYMATYYNVVSEENPNTCVIC 124

[Close]

BLAST of Carg22132 vs. TAIR10
Match: AT1G01490.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 5.6e-07
Identity = 26/43 (60.47%), Postives = 34/43 (79.07%), Query Frame = 0


Query: 1  MEMKEKKMTVIGEVDPICMVRKLRRF-RQTEIISVGPPKEEEK 43
          M+MKEKK+TVIG VDP+ +V KLR++   T+I+ VGP KE EK
Sbjct: 34 MDMKEKKLTVIGTVDPVNVVSKLRKYWPMTDIVLVGPAKEPEK 76

[Close]

BLAST of Carg22132 vs. TAIR10
Match: AT5G52740.1 (Copper transport protein family)

HSP 1 Score: 48.9 bits (115), Expect = 1.6e-06
Identity = 24/44 (54.55%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 1  MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKK 45
          +E+K+ K+TV GE+D   +V+KL++   TE ISVGP KE EKKK
Sbjct: 32 VEVKDGKLTVTGEIDAYMIVKKLKKICHTEFISVGPVKEPEKKK 75

[Close]

BLAST of Carg22132 vs. TAIR10
Match: AT5G52760.1 (Copper transport protein family)

HSP 1 Score: 46.2 bits (108), Expect = 1.1e-05
Identity = 34/89 (38.20%), Postives = 44/89 (49.44%), Query Frame = 0


Query: 4   KEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNY-------Y 63
           K  KMT++GEVD   +V KLR+   TEI+SV   K   KK   +K  E + Y       Y
Sbjct: 38  KSGKMTIVGEVDVPAVVMKLRKLCNTEIVSVDDVKPPVKKPEPEKPAESIAYPVPMNYAY 97

Query: 64  RYNVPFYSVQPYEQP---------PTCVI 77
           ++N P Y+   Y QP         P CVI
Sbjct: 98  QFN-PAYANSYYHQPYGNCRVVDEPNCVI 125

[Close]

BLAST of Carg22132 vs. TAIR10
Match: AT5G23760.1 (Copper transport protein family)

HSP 1 Score: 44.7 bits (104), Expect = 3.1e-05
Identity = 19/39 (48.72%), Postives = 32/39 (82.05%), Query Frame = 0


Query: 2  EMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEE 41
          +MK++K+TVIG +D + +V+KL++  + ++ISVGP KEE
Sbjct: 37 DMKDQKLTVIGLMDAVAVVKKLKKVGKVDLISVGPAKEE 75

[Close]

BLAST of Carg22132 vs. Swiss-Prot
Match: sp|O03982|HIP39_ARATH (Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX=3702 GN=HIPP39 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.0e-05
Identity = 26/43 (60.47%), Postives = 34/43 (79.07%), Query Frame = 0


Query: 1  MEMKEKKMTVIGEVDPICMVRKLRRF-RQTEIISVGPPKEEEK 43
          M+MKEKK+TVIG VDP+ +V KLR++   T+I+ VGP KE EK
Sbjct: 34 MDMKEKKLTVIGTVDPVNVVSKLRKYWPMTDIVLVGPAKEPEK 76

[Close]

BLAST of Carg22132 vs. Swiss-Prot
Match: sp|Q9LTE3|HIP12_ARATH (Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX=3702 GN=HIPP12 PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 2.9e-05
Identity = 24/44 (54.55%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 1  MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKK 45
          +E+K+ K+TV GE+D   +V+KL++   TE ISVGP KE EKKK
Sbjct: 32 VEVKDGKLTVTGEIDAYMIVKKLKKICHTEFISVGPVKEPEKKK 75

[Close]

BLAST of Carg22132 vs. Swiss-Prot
Match: sp|Q9LTE1|HIP14_ARATH (Heavy metal-associated isoprenylated plant protein 14 OS=Arabidopsis thaliana OX=3702 GN=HIPP14 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 1.9e-04
Identity = 34/89 (38.20%), Postives = 44/89 (49.44%), Query Frame = 0


Query: 4   KEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNY-------Y 63
           K  KMT++GEVD   +V KLR+   TEI+SV   K   KK   +K  E + Y       Y
Sbjct: 38  KSGKMTIVGEVDVPAVVMKLRKLCNTEIVSVDDVKPPVKKPEPEKPAESIAYPVPMNYAY 97

Query: 64  RYNVPFYSVQPYEQP---------PTCVI 77
           ++N P Y+   Y QP         P CVI
Sbjct: 98  QFN-PAYANSYYHQPYGNCRVVDEPNCVI 125

[Close]

BLAST of Carg22132 vs. TrEMBL
Match: tr|A0A1Q3C7S5|A0A1Q3C7S5_CEPFO (HMA domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_19603 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-08
Identity = 39/91 (42.86%), Postives = 55/91 (60.44%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEE----------KKKAEDKIE 60
           ++MKEKK+TVIG+ DP+ +V KLR+  QTEI++VGP KEE+               ++I 
Sbjct: 34  IDMKEKKLTVIGDADPVNIVEKLRKQWQTEILTVGPAKEEKXXXXXXXXXXXXXXNEQIS 93

Query: 61  EVLNYYR----YNVPFYSVQPYEQPPTCVIC 78
           E+ N Y+    Y   +Y+V   E P TCVIC
Sbjct: 94  ELANAYKAYNPYMATYYNVVSEENPNTCVIC 124

[Close]

BLAST of Carg22132 vs. TrEMBL
Match: tr|A0A022RI30|A0A022RI30_ERYGU (Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv11b021772mg PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-08
Identity = 37/90 (41.11%), Postives = 54/90 (60.00%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKK--------KAEDKIEEV 60
           M+MKEKK+TV+G+ DPI +V KL+++   E+++VGP KE EKK        K  + IEE+
Sbjct: 33  MDMKEKKLTVVGDADPIKIVGKLKKYCHVELLTVGPSKEPEKKEGVKKEENKNSEPIEEL 92

Query: 61  LNYYRYNVPFYS-----VQPYEQPPTCVIC 78
           +  Y  + P Y+         E P +CVIC
Sbjct: 93  MKLYNNHFPHYTQYYRVYSAEENPNSCVIC 122

[Close]

BLAST of Carg22132 vs. TrEMBL
Match: tr|A0A022RIQ8|A0A022RIQ8_ERYGU (Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a020391mg PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-08
Identity = 37/90 (41.11%), Postives = 54/90 (60.00%), Query Frame = 0


Query: 1   MEMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKK--------KAEDKIEEV 60
           M+MKEKK+TV+G+ DPI +V KL+++   E+++VGP KE EKK        K  + IEE+
Sbjct: 34  MDMKEKKLTVVGDADPIKIVGKLKKYCHVELLTVGPSKEPEKKEGVKKEENKNSEPIEEL 93

Query: 61  LNYYRYNVPFYS-----VQPYEQPPTCVIC 78
           +  Y  + P Y+         E P +CVIC
Sbjct: 94  MKLYNNHFPHYTQYYHVYSAEENPNSCVIC 123

[Close]

BLAST of Carg22132 vs. TrEMBL
Match: tr|A0A2I4HF45|A0A2I4HF45_9ROSI (uncharacterized protein LOC109016874 OS=Juglans regia OX=51240 GN=LOC109016874 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-07
Identity = 37/79 (46.84%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 2   EMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNYYRYNVP 61
           ++++KKMTVIG+VDP+ +V KLR+   TEI+SVGP K+ E  K +D  E +  Y  YN  
Sbjct: 35  DLEDKKMTVIGDVDPVDIVSKLRKLCPTEILSVGPAKKVE-PKMDDMAELLKVYQAYNYN 94

Query: 62  FYSVQPY---EQPPTCVIC 78
            Y    Y   E+P +CVIC
Sbjct: 95  PYKATHYYAEEEPNSCVIC 112

[Close]

BLAST of Carg22132 vs. TrEMBL
Match: tr|A0A2I4G3X7|A0A2I4G3X7_9ROSI (uncharacterized protein LOC109004502 isoform X2 OS=Juglans regia OX=51240 GN=LOC109004502 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-07
Identity = 37/79 (46.84%), Postives = 51/79 (64.56%), Query Frame = 0


Query: 2   EMKEKKMTVIGEVDPICMVRKLRRFRQTEIISVGPPKEEEKKKAEDKIEEVLNYYRYNVP 61
           ++++KKMTVIG+VDP+ +V KLR+   TEI+SVGP K+ E  K +D  E +  Y  YN  
Sbjct: 80  DLEDKKMTVIGDVDPVDIVSKLRKLCPTEILSVGPAKKVE-PKMDDMAELLKVYQAYNYN 139

Query: 62  FYSVQPY---EQPPTCVIC 78
            Y    Y   E+P +CVIC
Sbjct: 140 PYKATHYYAEEEPNSCVIC 157

The following BLAST results are available for this feature:

BLAST of Carg22132 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945780.1	8.0e-36	100.00	heavy metal-associated isoprenylated plant protein 39-like [Cucurbita moschata]	[more]
XP_016201007.1	2.2e-09	45.78	heavy metal-associated isoprenylated plant protein 39 [Arachis ipaensis] >XP_025...	[more]
XP_015967352.1	2.9e-09	44.58	heavy metal-associated isoprenylated plant protein 39 [Arachis duranensis]	[more]
XP_010671618.1	1.4e-08	48.15	PREDICTED: heavy metal-associated isoprenylated plant protein 39 [Beta vulgaris ...	[more]
GAV76128.1	2.4e-08	42.86	HMA domain-containing protein [Cephalotus follicularis]	[more]

BLAST of Carg22132 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G01490.1	5.6e-07	60.47	Heavy metal transport/detoxification superfamily protein	[more]
AT5G52740.1	1.6e-06	54.55	Copper transport protein family	[more]
AT5G52760.1	1.1e-05	38.20	Copper transport protein family	[more]
AT5G23760.1	3.1e-05	48.72	Copper transport protein family	[more]

BLAST of Carg22132 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O03982\|HIP39_ARATH	1.0e-05	60.47	Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX...	[more]
sp\|Q9LTE3\|HIP12_ARATH	2.9e-05	54.55	Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX...	[more]
sp\|Q9LTE1\|HIP14_ARATH	1.9e-04	38.20	Heavy metal-associated isoprenylated plant protein 14 OS=Arabidopsis thaliana OX...	[more]

BLAST of Carg22132 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1Q3C7S5\|A0A1Q3C7S5_CEPFO	1.6e-08	42.86	HMA domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_1960...	[more]
tr\|A0A022RI30\|A0A022RI30_ERYGU	2.7e-08	41.11	Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv11b021772mg P...	[more]
tr\|A0A022RIQ8\|A0A022RIQ8_ERYGU	2.7e-08	41.11	Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a020391mg PE...	[more]
tr\|A0A2I4HF45\|A0A2I4HF45_9ROSI	1.4e-07	46.84	uncharacterized protein LOC109016874 OS=Juglans regia OX=51240 GN=LOC109016874 P...	[more]
tr\|A0A2I4G3X7\|A0A2I4G3X7_9ROSI	1.4e-07	46.84	uncharacterized protein LOC109004502 isoform X2 OS=Juglans regia OX=51240 GN=LOC...	[more]

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22132-RA	Carg22132-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 1..35 e-value: 2.9E-6 score: 29.4
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 1..68
None	No IPR available	PANTHER	PTHR22814:SF279	SUBFAMILY NOT NAMED	coord: 1..68

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg22132	Cla97C05G090530	Watermelon (97103) v2	carwmbB0772

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg22132	Silver-seed gourd	carcarB583
Carg22132	Silver-seed gourd	carcarB594
Carg22132	Cucumber (Gy14) v2	carcgybB0733
Carg22132	Cucumber (Gy14) v1	carcgyB0828
Carg22132	Cucurbita maxima (Rimu)	carcmaB1031
Carg22132	Cucurbita maxima (Rimu)	carcmaB1032
Carg22132	Cucurbita moschata (Rifu)	carcmoB1002
Carg22132	Cucurbita moschata (Rifu)	carcmoB1003
Carg22132	Cucurbita pepo (Zucchini)	carcpeB1038
Carg22132	Cucurbita pepo (Zucchini)	carcpeB1039
Carg22132	Wild cucumber (PI 183967)	carcpiB0771
Carg22132	Cucumber (Chinese Long) v2	carcuB0754
Carg22132	Cucumber (Chinese Long) v3	carcucB0774
Carg22132	Bottle gourd (USVL1VR-Ls)	carlsiB675
Carg22132	Melon (DHL92) v3.5.1	carmeB0732
Carg22132	Melon (DHL92) v3.6.1	carmedB0743
Carg22132	Watermelon (Charleston Gray)	carwcgB0715
Carg22132	Watermelon (97103) v1	carwmB0736
Carg22132	Wax gourd	carwgoB0869