Carg21792 (gene) Silver-seed gourd

Overview
NameCarg21792
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description3-oxo-Delta(4,5)-steroid 5-beta-reductase
LocationCucurbita_argyrosperma_scaffold_198 : 298631 .. 299306 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTGGTGGTGGGCCGGAGCGATTGGCGCTGCTAAGGTAACTAGTAACTCCATATCTGTACCCATTCCTCTGTTTCTATCTCTGGAATGCCTCCACGTTTCTCTTATCGACGTCTCTGTTCCCGATTTTGCAGAAGAAATTTGAAGAAGGTGAACCTCCGAGGAGCTTTGAGGGCGTCGCCCTGGTGATCGGCGTTACTGGTATCGTTGGCAACAGTCTCGCCGAGATTCTCCCTCTTTCCGACACTCCCGGCGGTCCATGGAAGGTCTACGGCGTCGCTCGGAGGTCGAGGCCAAGTTGGAACGCCGACCATCCCATCGAATACATTCAATGTGATATCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGCAGCTCGTTGATGTGACTCATCTCTTCTATGTCACATGGACCAAGCGATCCACCGAGCTCGAGAACTGCGAGGCCAACGTTAAAATGCTTCGCAACGTGTTGCGATCAGTGATCCCCAACGCGCCCAATCTCCGCCATATCTGCCTTCAAACTGGTACTAAGCACTACGTCGGATCCTTCGAGAGCATTGTCAACAAATCCGTGCAGCCGCACGATTCGCCCTTCACGGAGGATTTGCCTCGATTGAAATGCCCAAATTTCTATTACCCGCAAGAAGATTTGTTGTTGAAGAAATTGTAA

mRNA sequence

ATGAGCTGGTGGTGGGCCGGAGCGATTGGCGCTGCTAAGAAGAAATTTGAAGAAGGTGAACCTCCGAGGAGCTTTGAGGGCGTCGCCCTGGTGATCGGCGTTACTGGTATCGTTGGCAACAGTCTCGCCGAGATTCTCCCTCTTTCCGACACTCCCGGCGGTCCATGGAAGGTCTACGGCGTCGCTCGGAGGTCGAGGCCAAGTTGGAACGCCGACCATCCCATCGAATACATTCAATGTGATATCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGCAGCTCGTTGATGTGACTCATCTCTTCTATGTCACATGGACCAAGCGATCCACCGAGCTCGAGAACTGCGAGGCCAACGTTAAAATGCTTCGCAACGTGTTGCGATCAGTGATCCCCAACGCGCCCAATCTCCGCCATATCTGCCTTCAAACTGGTACTAAGCACTACGTCGGATCCTTCGAGAGCATTGTCAACAAATCCGTGCAGCCGCACGATTCGCCCTTCACGGAGGATTTGCCTCGATTGAAATGCCCAAATTTCTATTACCCGCAAGAAGATTTGTTGTTGAAGAAATTGTAA

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCGATTGGCGCTGCTAAGAAGAAATTTGAAGAAGGTGAACCTCCGAGGAGCTTTGAGGGCGTCGCCCTGGTGATCGGCGTTACTGGTATCGTTGGCAACAGTCTCGCCGAGATTCTCCCTCTTTCCGACACTCCCGGCGGTCCATGGAAGGTCTACGGCGTCGCTCGGAGGTCGAGGCCAAGTTGGAACGCCGACCATCCCATCGAATACATTCAATGTGATATCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGCAGCTCGTTGATGTGACTCATCTCTTCTATGTCACATGGACCAAGCGATCCACCGAGCTCGAGAACTGCGAGGCCAACGTTAAAATGCTTCGCAACGTGTTGCGATCAGTGATCCCCAACGCGCCCAATCTCCGCCATATCTGCCTTCAAACTGGTACTAAGCACTACGTCGGATCCTTCGAGAGCATTGTCAACAAATCCGTGCAGCCGCACGATTCGCCCTTCACGGAGGATTTGCCTCGATTGAAATGCCCAAATTTCTATTACCCGCAAGAAGATTTGTTGTTGAAGAAATTGTAA

Protein sequence

MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANVKMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNFYYPQEDLLLKKL
Homology
[Close]
BLAST of Carg21792 vs. NCBI nr
Match: XP_022936254.1 (uncharacterized protein LOC111442928 [Cucurbita moschata])

HSP 1 Score: 397.1 bits (1019), Expect = 3.3e-107
Identity = 187/192 (97.40%), Postives = 190/192 (98.96%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QEDLL +++
Sbjct: 181 YYTQEDLLFEEI 192

[Close]
BLAST of Carg21792 vs. NCBI nr
Match: XP_023535925.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 393.7 bits (1010), Expect = 3.7e-106
Identity = 186/192 (96.88%), Postives = 189/192 (98.44%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQ HDSPFTEDLPRLKCPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQRHDSPFTEDLPRLKCPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QEDLL +++
Sbjct: 181 YYTQEDLLFEEI 192

[Close]
BLAST of Carg21792 vs. NCBI nr
Match: XP_022977039.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 379.4 bits (973), Expect = 7.2e-102
Identity = 180/192 (93.75%), Postives = 184/192 (95.83%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK F+E EPPR FEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKTFDEDEPPRRFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           KMLRNVLRSVI NAPNLRHICLQTGTKHYVGSFESIVNKSVQP  SPFTEDLPRLKCPNF
Sbjct: 121 KMLRNVLRSVISNAPNLRHICLQTGTKHYVGSFESIVNKSVQPPGSPFTEDLPRLKCPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QEDLL +++
Sbjct: 181 YYTQEDLLFEEI 192

[Close]
BLAST of Carg21792 vs. NCBI nr
Match: XP_004152001.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothetical protein Csa_3G610790 [Cucumis sativus])

HSP 1 Score: 359.4 bits (921), Expect = 7.7e-96
Identity = 166/192 (86.46%), Postives = 176/192 (91.67%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIG     FEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGXXXXXFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+TE+ENCEANV
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS Q HD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QEDLL +++
Sbjct: 181 YYKQEDLLWEEI 192

[Close]
BLAST of Carg21792 vs. NCBI nr
Match: XP_008447374.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo])

HSP 1 Score: 352.1 bits (902), Expect = 1.2e-93
Identity = 161/192 (83.85%), Postives = 174/192 (90.62%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIG     FEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGXXXXXFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+ E++NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES++NKS Q H+ PFTEDLPRL+CPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QEDLL +++
Sbjct: 181 YYKQEDLLFEEI 192

[Close]
BLAST of Carg21792 vs. TAIR10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 307.8 bits (787), Expect = 4.8e-84
Identity = 141/192 (73.44%), Postives = 161/192 (83.85%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +E EP +SFE VAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP+WNADHPI+YIQCD+SD ED  +KLS L DVTH+FYVTWT R +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F    N     HD PFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QED+L +++
Sbjct: 181 YYTQEDILFEEI 189

[Close]
BLAST of Carg21792 vs. TAIR10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 109.8 bits (273), Expect = 1.9e-24
Identity = 69/173 (39.88%), Postives = 98/173 (56.65%), Query Frame = 0 

Query: 26  EGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRSRPSWNA-DHPIEYIQCDISD 85
           E VAL+ GVTG+VG  + + L L   PG  W++YGVAR   P  N+      +I CD+ +
Sbjct: 16  ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVAR--NPEINSMTKMYNFISCDLLN 75

Query: 86  PEDAEAKLSQLVD-VTHLFYVTWTKR---STELENCEANVKMLRNVLRSVIPNAPNLRHI 145
             + + +LS L D V+H+F+VTW+      T+ E C  N  ML N L +++PNA  L+H 
Sbjct: 76  ASETKQRLSPLQDIVSHVFWVTWSGEFPLDTD-ECCVQNKTMLMNALDAILPNAKRLKHF 135

Query: 146 CLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKC-PNFYYPQEDLLLKKL 193
            LQTG KHYV   E  + +        ++E+ PR     NFYY  EDLL +K+
Sbjct: 136 SLQTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKI 182

[Close]
BLAST of Carg21792 vs. Swiss-Prot
Match: sp|Q9STX2|VEP1_ARATH (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 8.7e-83
Identity = 141/192 (73.44%), Postives = 161/192 (83.85%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +E EP +SFE VAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP+WNADHPI+YIQCD+SD ED  +KLS L DVTH+FYVTWT R +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F    N     HD PFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QED+L +++
Sbjct: 181 YYTQEDILFEEI 189

[Close]
BLAST of Carg21792 vs. Swiss-Prot
Match: sp|A0A1C9CX66|CYC2_CAMAC (Iridoid synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 2.2e-78
Identity = 133/190 (70.00%), Postives = 153/190 (80.53%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA+KKFEE + PR  + VAL+IGVTGIVGNSLAEILP+SDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP+WNADHP+EYIQCDISD  D   KLS L DVTH+F+VTW  R TE E CE N 
Sbjct: 61  VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
            MLRNVL ++IP A NL HICLQTG KHY+G FE+     ++PH+ PFTED+PRL  PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAF--GKIKPHEPPFTEDMPRLNAPNF 180

Query: 181 YYPQEDLLLK 191
           YY  ED+L++
Sbjct: 181 YYTLEDMLVE 188

[Close]
BLAST of Carg21792 vs. Swiss-Prot
Match: sp|Q6PQJ9|5BPOR_DIGLA (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 8.4e-78
Identity = 132/192 (68.75%), Postives = 158/192 (82.29%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EE +       VAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR+RP+W+ D+PI Y+QCDISDP+D++AKLS L DVTH+FYVTW  RSTE ENCEAN 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G FES     ++ HD P+TEDLPRLK  NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYPQEDLLLKKL 193
           YY  ED++L+++
Sbjct: 181 YYDLEDIMLEEV 190

[Close]
BLAST of Carg21792 vs. Swiss-Prot
Match: sp|A0A1C9CX56|CYC1_CAMAC (Iridoid synthase OS=Camptotheca acuminata OX=16922 GN=CYC1 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-69
Identity = 122/191 (63.87%), Postives = 148/191 (77.49%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWA ++G A+K+F+E     S++ V L+IGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWARSLG-AQKEFKENGALTSYQSVGLIIGVTGIVGNSLAEILPLPDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP+WN DHPIEYIQCD++  ED  AKLS L ++TH+FYV WT      E+CE N 
Sbjct: 61  VARRPRPAWNTDHPIEYIQCDVASTEDTLAKLSPLTNITHIFYVAWTGS----EDCEVNE 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
            M  NVL++VIPNAPNLRHIC+QTG KHY G  +   +  +QPHDSPFTEDLPRL  PNF
Sbjct: 121 TMFHNVLKAVIPNAPNLRHICIQTGIKHYTGPLDP--DGGIQPHDSPFTEDLPRLNAPNF 180

Query: 181 YYPQEDLLLKK 192
           Y+  ED+L+++
Sbjct: 181 YHNLEDILIEE 184

[Close]
BLAST of Carg21792 vs. Swiss-Prot
Match: sp|K7WDL7|IRIS_CATRO (Iridoid synthase OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.9e-61
Identity = 113/194 (58.25%), Postives = 140/194 (72.16%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAK---KKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWK 60
           MSWWW  +IGA K    + +E    +S++ VALV+GVTGIVG+SLAE+L L DTPGGPWK
Sbjct: 1   MSWWWKRSIGAGKNLPNQNKENGVCKSYKSVALVVGVTGIVGSSLAEVLKLPDTPGGPWK 60

Query: 61  VYGVARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCE 120
           VYGVARR  P W A  P+EYIQCD+SD ++  +KLS L D+TH+FYV+W       E+C+
Sbjct: 61  VYGVARRPCPVWLAKKPVEYIQCDVSDNQETISKLSPLKDITHIFYVSWIGS----EDCQ 120

Query: 121 ANVKMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKC 180
            N  M +N+L SVIPNA NL+H+CLQTG KHY G FE      V PHDSPFTEDLPRL  
Sbjct: 121 TNATMFKNILNSVIPNASNLQHVCLQTGIKHYFGIFEE--GSKVVPHDSPFTEDLPRLNV 180

Query: 181 PNFYYPQEDLLLKK 192
           PNFY+  ED+L ++
Sbjct: 181 PNFYHDLEDILYEE 188

[Close]
BLAST of Carg21792 vs. TrEMBL
Match: tr|A0A0A0L8Y0|A0A0A0L8Y0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 5.1e-96
Identity = 166/192 (86.46%), Postives = 176/192 (91.67%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIG     FEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGXXXXXFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+TE+ENCEANV
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS Q HD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QEDLL +++
Sbjct: 181 YYKQEDLLWEEI 192

[Close]
BLAST of Carg21792 vs. TrEMBL
Match: tr|A0A1S3BHA7|A0A1S3BHA7_CUCME (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 8.1e-94
Identity = 161/192 (83.85%), Postives = 174/192 (90.62%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIG     FEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGXXXXXFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+ E++NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES++NKS Q H+ PFTEDLPRL+CPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QEDLL +++
Sbjct: 181 YYKQEDLLFEEI 192

[Close]
BLAST of Carg21792 vs. TrEMBL
Match: tr|A0A1U8JBY3|A0A1U8JBY3_GOSHI (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Gossypium hirsutum OX=3635 GN=LOC107905623 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.8e-86
Identity = 150/192 (78.12%), Postives = 165/192 (85.94%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE E PRSF+ V L+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEAPRSFQSVGLIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RP+WNADHPIEYIQCDISDP D E+KLSQL DVTH+FYV+W  R++E ENCE N 
Sbjct: 61  VARRPRPNWNADHPIEYIQCDISDPNDTESKLSQLTDVTHIFYVSWINRTSEAENCEING 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
            MLRNVL SVIPNAPNLRHICLQTG KHY+G FE +    +QPHD PFTEDLPRL  PNF
Sbjct: 121 SMLRNVLHSVIPNAPNLRHICLQTGAKHYLGPFELL--GKIQPHDPPFTEDLPRLNAPNF 180

Query: 181 YYPQEDLLLKKL 193
           YY QED+L +++
Sbjct: 181 YYHQEDILFEEI 190

[Close]
BLAST of Carg21792 vs. TrEMBL
Match: tr|V7ASV6|V7ASV6_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_009G070100g PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 8.2e-86
Identity = 151/191 (79.06%), Postives = 166/191 (86.91%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE EPPRSFE V LVIGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVGLVIGVTGIVGNSLAEILPLHDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RPSWNADHPIEY+QCD+SDP DAE+KLS L DVTH+F+V+WT R TE ENCE N 
Sbjct: 61  VARRPRPSWNADHPIEYVQCDVSDPADAESKLSVLTDVTHVFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
            ML+NVLR+VIPNAPNLRH+ LQTGTKHY+GSFESI    +Q H+ PFTEDLPRL  PNF
Sbjct: 121 AMLQNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYPQEDLLLKK 192
           YY QED+L ++
Sbjct: 181 YYNQEDILFEE 189

[Close]
BLAST of Carg21792 vs. TrEMBL
Match: tr|V7AWY5|V7AWY5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_009G070100g PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 8.2e-86
Identity = 151/191 (79.06%), Postives = 166/191 (86.91%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE EPPRSFE V LVIGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVGLVIGVTGIVGNSLAEILPLHDTPGGPWKVYG 60

Query: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120
           VARR RPSWNADHPIEY+QCD+SDP DAE+KLS L DVTH+F+V+WT R TE ENCE N 
Sbjct: 61  VARRPRPSWNADHPIEYVQCDVSDPADAESKLSVLTDVTHVFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180
            ML+NVLR+VIPNAPNLRH+ LQTGTKHY+GSFESI    +Q H+ PFTEDLPRL  PNF
Sbjct: 121 AMLQNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYPQEDLLLKK 192
           YY QED+L ++
Sbjct: 181 YYNQEDILFEE 189

The following BLAST results are available for this feature:
BLAST of Carg21792 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022936254.13.3e-10797.40uncharacterized protein LOC111442928 [Cucurbita moschata][more]
XP_023535925.13.7e-10696.883-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo][more]
XP_022977039.17.2e-10293.753-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima][more]
XP_004152001.17.7e-9686.46PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303... [more]
XP_008447374.11.2e-9383.85PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo][more]
BLAST of Carg21792 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G24220.14.8e-8473.44NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G58750.11.9e-2439.88NAD(P)-binding Rossmann-fold superfamily protein[more]
BLAST of Carg21792 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9STX2|VEP1_ARATH8.7e-8373.443-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
sp|A0A1C9CX66|CYC2_CAMAC2.2e-7870.00Iridoid synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1[more]
sp|Q6PQJ9|5BPOR_DIGLA8.4e-7868.753-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1[more]
sp|A0A1C9CX56|CYC1_CAMAC1.1e-6963.87Iridoid synthase OS=Camptotheca acuminata OX=16922 GN=CYC1 PE=1 SV=1[more]
sp|K7WDL7|IRIS_CATRO1.9e-6158.25Iridoid synthase OS=Catharanthus roseus OX=4058 PE=1 SV=1[more]
BLAST of Carg21792 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0L8Y0|A0A0A0L8Y0_CUCSA5.1e-9686.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 PE=4 SV=1[more]
tr|A0A1S3BHA7|A0A1S3BHA7_CUCME8.1e-9483.853-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983... [more]
tr|A0A1U8JBY3|A0A1U8JBY3_GOSHI2.8e-8678.133-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Gossypium hirsutum OX=3635 GN=... [more]
tr|V7ASV6|V7ASV6_PHAVU8.2e-8679.06Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_009G070100g PE=4 ... [more]
tr|V7AWY5|V7AWY5_PHAVU8.2e-8679.06Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_009G070100g PE=4 ... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0050662coenzyme binding
Vocabulary: INTERPRO
TermDefinition
IPR036291NAD(P)-bd_dom_sf
IPR001509Epimerase_deHydtase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050662 coenzyme binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21792-RACarg21792-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 29..156
e-value: 1.5E-5
score: 24.5
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 27..192
e-value: 4.2E-50
score: 172.7
NoneNo IPR availablePANTHERPTHR32487:SF03-OXO-DELTA 4,5 -STEROID 5-BETA-REDUCTASEcoord: 26..190
NoneNo IPR availablePANTHERPTHR32487FAMILY NOT NAMEDcoord: 26..190
IPR036291NAD(P)-binding domain superfamilySUPERFAMILYSSF51735NAD(P)-binding Rossmann-fold domainscoord: 28..173

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg21792Silver-seed gourdcarcarB216
Carg21792Silver-seed gourdcarcarB344
Carg21792Silver-seed gourdcarcarB346
Carg21792Cucumber (Gy14) v2carcgybB0330
Carg21792Cucumber (Gy14) v2carcgybB0331
Carg21792Cucumber (Gy14) v1carcgyB0378
Carg21792Cucumber (Gy14) v1carcgyB0379
Carg21792Cucurbita maxima (Rimu)carcmaB0448
Carg21792Cucurbita maxima (Rimu)carcmaB0449
Carg21792Cucurbita maxima (Rimu)carcmaB0450
Carg21792Cucurbita maxima (Rimu)carcmaB0452
Carg21792Cucurbita moschata (Rifu)carcmoB0443
Carg21792Cucurbita moschata (Rifu)carcmoB0439
Carg21792Cucurbita moschata (Rifu)carcmoB0440
Carg21792Cucurbita moschata (Rifu)carcmoB0441
Carg21792Cucurbita pepo (Zucchini)carcpeB0446
Carg21792Cucurbita pepo (Zucchini)carcpeB0447
Carg21792Cucurbita pepo (Zucchini)carcpeB0448
Carg21792Cucurbita pepo (Zucchini)carcpeB0449
Carg21792Wild cucumber (PI 183967)carcpiB0352
Carg21792Wild cucumber (PI 183967)carcpiB0353
Carg21792Cucumber (Chinese Long) v2carcuB0346
Carg21792Cucumber (Chinese Long) v2carcuB0347
Carg21792Cucumber (Chinese Long) v3carcucB0351
Carg21792Cucumber (Chinese Long) v3carcucB0352
Carg21792Bottle gourd (USVL1VR-Ls)carlsiB294
Carg21792Bottle gourd (USVL1VR-Ls)carlsiB295
Carg21792Melon (DHL92) v3.5.1carmeB0324
Carg21792Melon (DHL92) v3.5.1carmeB0325
Carg21792Melon (DHL92) v3.6.1carmedB0334
Carg21792Melon (DHL92) v3.6.1carmedB0333
Carg21792Watermelon (Charleston Gray)carwcgB0314
Carg21792Watermelon (Charleston Gray)carwcgB0315
Carg21792Watermelon (97103) v1carwmB0310
Carg21792Watermelon (97103) v1carwmB0313
Carg21792Watermelon (97103) v2carwmbB0330
Carg21792Watermelon (97103) v2carwmbB0332
Carg21792Wax gourdcarwgoB0404
Carg21792Wax gourdcarwgoB0406