Carg21335 (gene) Silver-seed gourd

BLAST of Carg21335 vs. NCBI nr
Match: XP_022940845.1 (centrosomal protein of 83 kDa [Cucurbita moschata])

HSP 1 Score: 1359.0 bits (3516), Expect = 0.0e+00
Identity = 810/817 (99.14%), Postives = 812/817 (99.39%), Query Frame = 0

Query: 1   MDVELNNQVEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNST 60
           MDVELNNQVEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNST
Sbjct: 1   MDVELNNQVEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNST 60

Query: 61  ADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ 120
           ADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ
Sbjct: 61  ADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ 120

Query: 121 NSLQITDPSQVATITNTPASAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAXXXX 180
           NSLQITDPSQVATI NTP SAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDA XXX
Sbjct: 121 NSLQITDPSQVATIMNTPGSAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAHXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED 240

Query: 241 VIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI 300
           VIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI
Sbjct: 241 VIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI 300

Query: 301 QRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV 360
           QRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV
Sbjct: 301 QRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV 360

Query: 361 EALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHL 420
           EALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHL
Sbjct: 361 EALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHL 420

Query: 421 SNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLA 480
           SNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLA
Sbjct: 421 SNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLA 480

Query: 481 LVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSV 540
           LVEEKKREIEENDSLIDAVEKRISVAI GFQHVHSNMDAKYDSLQSTLSQLQLESQSLSV
Sbjct: 481 LVEEKKREIEENDSLIDAVEKRISVAIPGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSV 540

Query: 541 KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKASLSQS 600
           KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAY+EIARLRKFLMSNILKIREDKASLSQS
Sbjct: 541 KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYKEIARLRKFLMSNILKIREDKASLSQS 600

Query: 601 EDKLSKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVA 660
           EDKLSKDVQM XXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVA
Sbjct: 601 EDKLSKDVQMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVA 660

Query: 661 AAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQS 720
           AAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQS
Sbjct: 661 AAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQS 720

Query: 721 KEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH 780
           KEKEVAKARF+RLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH
Sbjct: 721 KEKEVAKARFKRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH 780

Query: 781 EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN 818
           EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN
Sbjct: 781 EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN 817

BLAST of Carg21335 vs. NCBI nr
Match: XP_023525124.1 (centrosomal protein of 83 kDa [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1327.8 bits (3435), Expect = 0.0e+00
Identity = 780/817 (95.47%), Postives = 782/817 (95.72%), Query Frame = 0

Query: 1   MDVELNNQVEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNST 60
           MDVELNNQ+EDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNS 
Sbjct: 1   MDVELNNQLEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNSP 60

Query: 61  ADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ 120
           ADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ
Sbjct: 61  ADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ 120

Query: 121 NSLQITDPSQVATITNTPASAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAXXXX 180
           NSLQITDPSQVATI NTP SAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDA XXX
Sbjct: 121 NSLQITDPSQVATIMNTPGSAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAHXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED 240

Query: 241 VIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI 300
           VIMGSPNQQDANRLEK+SVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI
Sbjct: 241 VIMGSPNQQDANRLEKSSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI 300

Query: 301 QRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV 360
           QRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV
Sbjct: 301 QRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV 360

Query: 361 EALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHL 420
           EAL DAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHL
Sbjct: 361 EALFDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHL 420

Query: 421 SNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLA 480
           SNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLA
Sbjct: 421 SNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLA 480

Query: 481 LVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSV 540
           LVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSV
Sbjct: 481 LVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSV 540

Query: 541 KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKASLSQS 600
           KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILK REDKASLSQS
Sbjct: 541 KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKTREDKASLSQS 600

Query: 601 EDKLSKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVA 660
           EDKLSKDVQML            XXXXXXXXXXX     KQRISFIDKRVPELEAEKKVA
Sbjct: 601 EDKLSKDVQMLQQEFHSASSSLQXXXXXXXXXXXDIVSSKQRISFIDKRVPELEAEKKVA 660

Query: 661 AAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQS 720
           AAGRNFKEAARVAA            CIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQS
Sbjct: 661 AAGRNFKEAARVAAXXXXXXXXXXXXCIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQS 720

Query: 721 KEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH 780
           KEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH
Sbjct: 721 KEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH 780

Query: 781 EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN 818
           EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN
Sbjct: 781 EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN 817

BLAST of Carg21335 vs. NCBI nr
Match: XP_022981337.1 (centrosomal protein of 83 kDa [Cucurbita maxima])

HSP 1 Score: 1283.5 bits (3320), Expect = 0.0e+00
Identity = 767/817 (93.88%), Postives = 775/817 (94.86%), Query Frame = 0

Query: 1   MDVELNNQVEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNST 60
           MDVELNNQVEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHP NST
Sbjct: 1   MDVELNNQVEDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPPNST 60

Query: 61  ADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ 120
           ADKVTT                 HLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ
Sbjct: 61  ADKVTTXXXXXXXXXXXXXXXXXHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQ 120

Query: 121 NSLQITDPSQVATITNTPASAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAXXXX 180
           NSLQITDPSQVATI NTP S VGEATERDRGVVSISRQISRRKRRPGLRIGYGRDA XXX
Sbjct: 121 NSLQITDPSQVATIMNTPGSVVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAHXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQED 240

Query: 241 VIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI 300
           VIMG PNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI
Sbjct: 241 VIMGCPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVI 300

Query: 301 QRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV 360
           QRRRKIMDNL ISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV
Sbjct: 301 QRRRKIMDNLNISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDV 360

Query: 361 EALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHL 420
           EALCDAMDSRMHEVLDFLIATEEKCDSLL+TFATDAANDVVLGLNTAEAESAKELEKWHL
Sbjct: 361 EALCDAMDSRMHEVLDFLIATEEKCDSLLQTFATDAANDVVLGLNTAEAESAKELEKWHL 420

Query: 421 SNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLA 480
           SNEVLEAKKMETEIESLIIQESCMVLN+SVELLVEDDKREKNVLCQRKAVLTDELEKLLA
Sbjct: 421 SNEVLEAKKMETEIESLIIQESCMVLNNSVELLVEDDKREKNVLCQRKAVLTDELEKLLA 480

Query: 481 LVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSV 540
           LVEEKKREIEENDSLI AVE+RISVA+SGFQH+HSNMDAKYDSLQSTLSQLQLESQSLSV
Sbjct: 481 LVEEKKREIEENDSLIGAVERRISVAVSGFQHLHSNMDAKYDSLQSTLSQLQLESQSLSV 540

Query: 541 KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKASLSQS 600
           KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYRE+ARLRKFLMSNILKIREDKASLSQS
Sbjct: 541 KRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREVARLRKFLMSNILKIREDKASLSQS 600

Query: 601 EDKLSKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVA 660
           EDKLSKDVQML    XXXXXXXXXXXXXXXXXXXXXXX KQRISFIDKRVPELEAEKKVA
Sbjct: 601 EDKLSKDVQMLQQEFXXXXXXXXXXXXXXXXXXXXXXXSKQRISFIDKRVPELEAEKKVA 660

Query: 661 AAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQS 720
           AAGRNFKEAARVAA            C+DIDRALLDLEKLEEKTR+TMKWLQETEVSIQS
Sbjct: 661 AAGRNFKEAARVAAXXXXXXXXXXXXCVDIDRALLDLEKLEEKTRETMKWLQETEVSIQS 720

Query: 721 KEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH 780
           KEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH
Sbjct: 721 KEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFH 780

Query: 781 EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN 818
           EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN
Sbjct: 781 EDELSTIPKHFISAELVFNLGREKLADLVASICHPEN 817

BLAST of Carg21335 vs. NCBI nr
Match: XP_022131371.1 (uncharacterized protein LOC111004611 [Momordica charantia])

HSP 1 Score: 998.0 bits (2579), Expect = 1.8e-287
Identity = 591/819 (72.16%), Postives = 643/819 (78.51%), Query Frame = 0

Query: 1   MDVELNNQV-EDENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNS 60
           MDVELN+Q+ ED NENDSLFEGMVLFDPSEY IQI PT E  DSD PGP +S+QP   NS
Sbjct: 1   MDVELNDQIEEDGNENDSLFEGMVLFDPSEYRIQIHPTRE--DSDDPGPIVSDQPDLPNS 60

Query: 61  TADKVTTTASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLD 120
            AD+VT                            FSDLTLVT  SMH    K QTQ QLD
Sbjct: 61  AADEVTXXXXXXXXXXXXXXXXXXXXXXXXXXXXFSDLTLVT--SMH----KDQTQIQLD 120

Query: 121 QNSLQITDPSQVATITNTPASAVGEATERDRG-VVSISRQISRRKRRPGLRIGYGRDAXX 180
           QNSL ITDP Q ATI   P S VGEA +RD G +VS+SRQ+SRRKRR GLRIGYGRDA  
Sbjct: 121 QNSLPITDPLQAATIMKIPGSVVGEAADRDPGLIVSVSRQMSRRKRRAGLRIGYGRDA-H 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVD--KMESPNFV 240
                                       L +IQPSASPPQTL+ H  SVD   +ES +FV
Sbjct: 181 TPNPTPDLHSTHFSNHIRGDDDDQHPDALPQIQPSASPPQTLAPHGPSVDNNNIESTSFV 240

Query: 241 SQEDVIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASR 300
           SQED I    NQQDA+R +KNSVE EV +SPEFKLEQVRIQISE L  ARNSVASVS SR
Sbjct: 241 SQEDHITERQNQQDADRHKKNSVEAEVRDSPEFKLEQVRIQISEKLGHARNSVASVSTSR 300

Query: 301 KDVIQRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNE 360
           K+VIQR+RKIMDNLKISLD+YS+LE+QLEEACEAEDFETAERLSESLASAE EKQAFL E
Sbjct: 301 KEVIQRKRKIMDNLKISLDRYSHLEKQLEEACEAEDFETAERLSESLASAEREKQAFLME 360

Query: 361 LKDVEALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELE 420
           LKD EALCDAMDSRMHEVLDFLIATEE CDSLL+TFA DAAN VVL LN A++ESA+ELE
Sbjct: 361 LKDAEALCDAMDSRMHEVLDFLIATEENCDSLLQTFAMDAANSVVLALNMADSESAEELE 420

Query: 421 KWHLSNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELE 480
           KWHLSNEVLEAKKM  EIESLII+ESCMVLNDS+ELLVE D+REK VLCQ+KA LTDELE
Sbjct: 421 KWHLSNEVLEAKKMGIEIESLIIRESCMVLNDSIELLVEGDRREKEVLCQKKAALTDELE 480

Query: 481 KLLALVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQ 540
           KLLA+VEEKKREIEENDS+IDAVEKRIS AISGFQHV SNMDAKY +LQSTLSQ+QLESQ
Sbjct: 481 KLLAMVEEKKREIEENDSVIDAVEKRISDAISGFQHVQSNMDAKYAALQSTLSQMQLESQ 540

Query: 541 SLSVKRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKAS 600
           +LS +RREI+EFL LEK+KG  LKKIA LS+EDAEAYRE+ARLRKFLMS ILK REDKA+
Sbjct: 541 TLSNRRREIEEFLTLEKEKGANLKKIAHLSVEDAEAYREVARLRKFLMSTILKTREDKAT 600

Query: 601 LSQSEDKLSKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAE 660
           L+Q+ED LSKDVQML                            KQRISFIDKRVPELEAE
Sbjct: 601 LTQTEDDLSKDVQMLQQEFHSARSSLQELSSRKSNIQQDIVSSKQRISFIDKRVPELEAE 660

Query: 661 KKVAAAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEV 720
           KKV AAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALL+LEKLEE+TR TMKWLQ+TE 
Sbjct: 661 KKVVAAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLELEKLEEETRGTMKWLQKTEE 720

Query: 721 SIQSKEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPI 780
            IQ KEKEVAKARFQRLL+IAGAAAA+GAAALE GDTGEANLL  E+E+ARCEA+KLQP+
Sbjct: 721 LIQLKEKEVAKARFQRLLIIAGAAAADGAAALELGDTGEANLLFVESESARCEARKLQPV 780

Query: 781 YNFHEDELSTIPKHFISAELVFNLGREKLADLVASICHP 816
           Y+FHEDE S IPKHFIS ELVFNLGREKLADLVASI  P
Sbjct: 781 YDFHEDEFSNIPKHFISLELVFNLGREKLADLVASIHDP 810

BLAST of Carg21335 vs. NCBI nr
Match: XP_002274237.1 (PREDICTED: myosin heavy chain, fast skeletal muscle [Vitis vinifera])

HSP 1 Score: 524.6 bits (1350), Expect = 5.9e-145
Identity = 370/811 (45.62%), Postives = 493/811 (60.79%), Query Frame = 0

Query: 11  DENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNSTADKVTTTASN 70
           + +E DSLFEGM+LF+PS+       T  DQD    G D +  P PL        T AS 
Sbjct: 4   EADEMDSLFEGMILFNPSQ-------TLADQD----GVDGNCDPIPL--------TDASA 63

Query: 71  SASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQNSLQITDPSQ 130
           S SS+          S+PLDE+LFSDLTL+T +           QF   +N L    P+ 
Sbjct: 64  SLSSS---------SSQPLDENLFSDLTLITPN-----------QF---RNPLLDPSPTP 123

Query: 131 VATITNTPASAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAXXXXXXXXXXXXXX 190
           ++T T    +             S+SRQISR+K+R  LRIGYGRD+              
Sbjct: 124 ISTSTTREETP------------SVSRQISRKKKRASLRIGYGRDSLNQLSLVDDAEVQS 183

Query: 191 XXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQEDVIMGS----- 250
                                 S  P +TL SH S     ++       D+++       
Sbjct: 184 SPSDDSHSQTKSF---------SLLPDETLDSHPSPPTTSQAILVREDHDLVLDDVQFKP 243

Query: 251 -PNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVIQRRR 310
            P Q +  +++    +T    S E   ++VR  ISE L  AR   AS+SASRKD ++RRR
Sbjct: 244 FPQQNEQEQIQLEEEKTTCSTSTEANFDRVRAIISEKLHHARQLAASISASRKDSMRRRR 303

Query: 311 KIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDVEALC 370
           K   NL ++  K   LE++LEEACEAEDFE AERLS+SLA AE +KQAF+  L+D EA C
Sbjct: 304 KAAQNLNLASTKSMELEKELEEACEAEDFEMAERLSQSLAGAEKDKQAFIAALRDAEAEC 363

Query: 371 DAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHLSNEV 430
           DA+DS+M EVL+  I  EE+C SLL  FATDAAN+       A+  S+KE+++W  S E 
Sbjct: 364 DAIDSKMQEVLERQITAEEECVSLLEQFATDAANNANEISKRAQFLSSKEMDQWFSSTEA 423

Query: 431 LEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLALVEE 490
           LE +K+E EIES +I ++ ++LNDS+E   ED++RE+  LC+RK  L DELEKLLALV E
Sbjct: 424 LEVRKLELEIESHLISDTRLLLNDSIEHAAEDERREREFLCKRKDTLMDELEKLLALVRE 483

Query: 491 KKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSVKRRE 550
           K+ EI END+ I AV+KRI+  +SGFQ +H +++ KYD+LQS+LSQ +LES++LS+K++E
Sbjct: 484 KEDEIAENDANIKAVDKRIADVVSGFQEMHLSINMKYDNLQSSLSQTELESEALSMKKKE 543

Query: 551 IDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKASLSQSEDKL 610
           ID+ L+ E+++G +L+++A++S ++A+AY E+  LRK    +I+K REDK  L+++E+KL
Sbjct: 544 IDDLLSEEEERGAKLRELARISADEAKAYEEVVGLRKSFALSIVKSREDKLRLAKTEEKL 603

Query: 611 SKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVAAAGR 670
           S++VQ+L           XXXXXXXXXXXX     KQRI FIDK VPELEAEKKVAAA R
Sbjct: 604 SEEVQILRQEVSAARASLXXXXXXXXXXXXEIASSKQRIFFIDKIVPELEAEKKVAAAAR 663

Query: 671 NFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQSKEKE 730
           NFK                         A+L+L KLEE+ +DT+ WL E +  I  KEKE
Sbjct: 664 NFKXXXXXXXXXXXXXXXXXXXXXXXXXAMLELGKLEEEIKDTVNWLVEADELILFKEKE 723

Query: 731 VAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFHEDEL 790
           VA ARFQRLLL+AGAA AE  AA+E GD  EANLLLAEAEAA  EAK L+  YNF E+E 
Sbjct: 724 VAMARFQRLLLVAGAAKAERLAAVELGDLEEANLLLAEAEAAEVEAKNLEQSYNFKEEEF 751

Query: 791 STIPKHFISAELVFNLGREKLADLVASICHP 816
             +PKHF+S ELV NLG ++LA+L AS+  P
Sbjct: 784 ENLPKHFMSMELVSNLGGKQLAELAASVHLP 751

BLAST of Carg21335 vs. TAIR10
Match: AT5G25070.1 (unknown protein)

HSP 1 Score: 219.9 bits (559), Expect = 5.6e-57
Identity = 247/809 (30.53%), Postives = 353/809 (43.63%), Query Frame = 0

Query: 13  NENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNSTADKVTTTASNS- 72
           ++ DSLFEGM LF           TP  Q S      +S+ P    + A + T   + S 
Sbjct: 5   DDMDSLFEGMELF-----------TPASQFSG--DSKVSSPPQSEETKAAEATLITAPSX 64

Query: 73  ---ASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQNSLQITDP 132
                                DE+LFSDLT+VT         +HQ +             
Sbjct: 65  XXXXXXXXXXXXXXXXXXXXXDENLFSDLTIVT-------PVQHQPE-----------PM 124

Query: 133 SQVATITNTPASAVGEATERDRGVVSISRQISRRKRR-PGLRIGYGRDAXXXXXXXXXXX 192
             V T   +PA   G             RQ+SRRK+R  GLRIGYGR             
Sbjct: 125 EAVITTHQSPAKNYG-------------RQVSRRKKRAAGLRIGYGR------------- 184

Query: 193 XXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQEDVIMGSPN 252
                              L + +  A                                 
Sbjct: 185 --------------HETNNLDEDEDDAV--------XXXXXXXXXXXXXXXXXXXXXXXX 244

Query: 253 QQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVIQRRRKIM 312
                      V T V  +   +LE V+ QI   L  +R+  ASV+++RK+ I+++R+  
Sbjct: 245 XXXXXXXXXXPVVTVVVGNGSSRLELVKAQIEAKLNRSRDLAASVTSARKNAIRKKRQAS 304

Query: 313 DNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDVEALCDAM 372
           +NL+++   +  LE+QLEEA E EDF+ AER+SESLA+ E ++ A L  L+  E+ CDA+
Sbjct: 305 ENLRLASTTHEELEKQLEEAIETEDFDAAERISESLAAKERDRLALLALLRQAESDCDAI 364

Query: 373 DSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHLSNEVLEA 432
           +S+M EVL   IA EE+   LLR+F TDA ND    L  AEA  + E+EKWH  +E +E 
Sbjct: 365 ESKMEEVLLSQIAAEEESACLLRSFGTDAENDAGSILEKAEAFYSDEMEKWHSCSEDVEV 424

Query: 433 KKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLALVEEKKR 492
           +K+E +IES+++    + LN  +E  VE D +EK +L ++K  L +ELE+LLALV+ K++
Sbjct: 425 RKVELDIESVVVDNVRLSLNGILEGSVEQDMKEKEILQKKKEHLANELEELLALVKAKEK 484

Query: 493 EIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSVKRREIDE 552
                                                                K++++DE
Sbjct: 485 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKKKDVDE 544

Query: 553 FLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKASLSQSEDKLSKD 612
           F+  EK++G +L+ +A++S ++A  Y E+ +LRK LMS + K RE++A L   E+     
Sbjct: 545 FMTSEKERGAKLRDLARVSADEACEYEEVIKLRKGLMSYVSKTREERAKLVNIEEXXXXX 604

Query: 613 VQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVAAAGRNFK 672
               XXXXXXXXXXX                   +I FI+KR+PELEAEKKVAA+ RNFK
Sbjct: 605 XXXXXXXXXXXXXXXKERSSKKSIIQQNITSFMDKIMFIEKRMPELEAEKKVAASTRNFK 664

Query: 673 EAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQSKEKEVAK 732
           EA R+A                                                  E+A 
Sbjct: 665 EAGRIAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXELAI 724

Query: 733 ARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAA----RCEAKKLQPIYNFHEDE 792
           +RFQRL + +G A AE +AALE  D  EANLLL EA+ A                     
Sbjct: 725 SRFQRLRIDSGTAKAERSAALELSDLEEANLLLEEAQEAXXXXXXXXXXXXXXXXXXXXX 734

Query: 793 LSTIPKHFISAELVFNLGREKLADLVASI 813
                + F+S EL+  +G +KL +LV S+
Sbjct: 785 XXXXXEVFVSMELIATVGLKKLQELVESV 734

BLAST of Carg21335 vs. TrEMBL
Match: tr|F6I6Y5|F6I6Y5_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_18s0122g00110 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 3.9e-145
Identity = 370/811 (45.62%), Postives = 493/811 (60.79%), Query Frame = 0

Query: 11  DENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNSTADKVTTTASN 70
           + +E DSLFEGM+LF+PS+       T  DQD    G D +  P PL        T AS 
Sbjct: 4   EADEMDSLFEGMILFNPSQ-------TLADQD----GVDGNCDPIPL--------TDASA 63

Query: 71  SASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQNSLQITDPSQ 130
           S SS+          S+PLDE+LFSDLTL+T +           QF   +N L    P+ 
Sbjct: 64  SLSSS---------SSQPLDENLFSDLTLITPN-----------QF---RNPLLDPSPTP 123

Query: 131 VATITNTPASAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAXXXXXXXXXXXXXX 190
           ++T T    +             S+SRQISR+K+R  LRIGYGRD+              
Sbjct: 124 ISTSTTREETP------------SVSRQISRKKKRASLRIGYGRDSLNQLSLVDDAEVQS 183

Query: 191 XXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFVSQEDVIMGS----- 250
                                 S  P +TL SH S     ++       D+++       
Sbjct: 184 SPSDDSHSQTKSF---------SLLPDETLDSHPSPPTTSQAILVREDHDLVLDDVQFKP 243

Query: 251 -PNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASRKDVIQRRR 310
            P Q +  +++    +T    S E   ++VR  ISE L  AR   AS+SASRKD ++RRR
Sbjct: 244 FPQQNEQEQIQLEEEKTTCSTSTEANFDRVRAIISEKLHHARQLAASISASRKDSMRRRR 303

Query: 311 KIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNELKDVEALC 370
           K   NL ++  K   LE++LEEACEAEDFE AERLS+SLA AE +KQAF+  L+D EA C
Sbjct: 304 KAAQNLNLASTKSMELEKELEEACEAEDFEMAERLSQSLAGAEKDKQAFIAALRDAEAEC 363

Query: 371 DAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELEKWHLSNEV 430
           DA+DS+M EVL+  I  EE+C SLL  FATDAAN+       A+  S+KE+++W  S E 
Sbjct: 364 DAIDSKMQEVLERQITAEEECVSLLEQFATDAANNANEISKRAQFLSSKEMDQWFSSTEA 423

Query: 431 LEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLALVEE 490
           LE +K+E EIES +I ++ ++LNDS+E   ED++RE+  LC+RK  L DELEKLLALV E
Sbjct: 424 LEVRKLELEIESHLISDTRLLLNDSIEHAAEDERREREFLCKRKDTLMDELEKLLALVRE 483

Query: 491 KKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSVKRRE 550
           K+ EI END+ I AV+KRI+  +SGFQ +H +++ KYD+LQS+LSQ +LES++LS+K++E
Sbjct: 484 KEDEIAENDANIKAVDKRIADVVSGFQEMHLSINMKYDNLQSSLSQTELESEALSMKKKE 543

Query: 551 IDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKASLSQSEDKL 610
           ID+ L+ E+++G +L+++A++S ++A+AY E+  LRK    +I+K REDK  L+++E+KL
Sbjct: 544 IDDLLSEEEERGAKLRELARISADEAKAYEEVVGLRKSFALSIVKSREDKLRLAKTEEKL 603

Query: 611 SKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVAAAGR 670
           S++VQ+L           XXXXXXXXXXXX     KQRI FIDK VPELEAEKKVAAA R
Sbjct: 604 SEEVQILRQEVSAARASLXXXXXXXXXXXXEIASSKQRIFFIDKIVPELEAEKKVAAAAR 663

Query: 671 NFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQSKEKE 730
           NFK                         A+L+L KLEE+ +DT+ WL E +  I  KEKE
Sbjct: 664 NFKXXXXXXXXXXXXXXXXXXXXXXXXXAMLELGKLEEEIKDTVNWLVEADELILFKEKE 723

Query: 731 VAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPIYNFHEDEL 790
           VA ARFQRLLL+AGAA AE  AA+E GD  EANLLLAEAEAA  EAK L+  YNF E+E 
Sbjct: 724 VAMARFQRLLLVAGAAKAERLAAVELGDLEEANLLLAEAEAAEVEAKNLEQSYNFKEEEF 751

Query: 791 STIPKHFISAELVFNLGREKLADLVASICHP 816
             +PKHF+S ELV NLG ++LA+L AS+  P
Sbjct: 784 ENLPKHFMSMELVSNLGGKQLAELAASVHLP 751

BLAST of Carg21335 vs. TrEMBL
Match: tr|A0A2R6PZC7|A0A2R6PZC7_ACTCH (Centromere protein like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc23447 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 1.0e-132
Identity = 282/544 (51.84%), Postives = 387/544 (71.14%), Query Frame = 0

Query: 268 EFKLEQVRIQISENLTSARNSVASVSASRKDVIQRRRKIMDNLKISLDKYSNLERQLEEA 327
           E + EQ+R QIS+ +   R  VASVSA+RKD  +RRRK  D L +   +Y+ LE++LEEA
Sbjct: 277 ELRFEQIRAQISQKIRHVRELVASVSAARKDSTRRRRKAADTLNLESSRYAQLEKELEEA 336

Query: 328 CEAEDFETAERLSESLASAEGEKQAFLNELKDVEALCDAMDSRMHEVLDFLIATEEKCDS 387
           CEAEDFETAER+SES+ASAE  K+     L+  EA CDA+DS+M EVL+  IA EE+C S
Sbjct: 337 CEAEDFETAERVSESVASAEKNKERLAIGLRLAEADCDAVDSKMQEVLEQQIAAEEECAS 396

Query: 388 LLRTFATDAANDVVLGLNTAEAESAKELEKWHLSNEVLEAKKMETEIESLIIQESCMVLN 447
           LL+  + DAA+D       A  ES +EL++W    E LE KKME +IES ++  + ++++
Sbjct: 397 LLKILSMDAASDADQLQENATVESERELDRWLSLTEDLELKKMELDIESHLMNSARLMVS 456

Query: 448 DSVELLVEDDKREKNVLCQRKAVLTDELEKLLALVEEKKREIEENDSLIDAVEKRISVAI 507
           DS+E  VEDD+R++ +LC+RK +LT+EL KLLALV+EK+ EI E D  I+ VEKRI+  +
Sbjct: 457 DSIEHSVEDDRRQRELLCKRKEMLTEELVKLLALVKEKEVEIAEIDLNIETVEKRIADVV 516

Query: 508 SGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSVKRREIDEFLNLEKDKGEQLKKIAQLSI 567
           S FQ V S++ +KYD+LQS LSQ++L++++L+ K++EID+FL+ EKD+  +L+++AQ+  
Sbjct: 517 SSFQEVQSSVKSKYDNLQSGLSQMELDNEALTRKKKEIDDFLSQEKDRARKLQELAQIFA 576

Query: 568 EDAEAYREIARLRKFLMSNILKIREDKASLSQSEDKLSKDVQMLXXXXXXXXXXXXXXXX 627
            +A  Y+E+  LRK L+  ILK REDK  L+++E+KLS+DV ML                
Sbjct: 577 NEASMYQEVVELRKSLVLFILKSREDKVRLAKAEEKLSEDVHMLRQEISATRASLQKLSS 636

Query: 628 XXXXXXXXXXXXKQRISFIDKRVPELEAEKKVAAAGRNFKEAARVAAEAKSLSNEKDSIC 687
                       KQR+ F+DKRVP+LE EKKVAAA RNFKEAAR+AAE+K+L  EK+S+ 
Sbjct: 637 NKYSFQQEIESLKQRLVFVDKRVPDLETEKKVAAAARNFKEAARIAAESKALGVEKESLQ 696

Query: 688 IDIDRALLDLEKLEEKTRDTMKWLQETEVSIQSKEKEVAKARFQRLLLIAGAAAAEGAAA 747
           I +  A+L+L K+EE   + +  LQETE  I +KEKEVA ARFQRLLLIAGAA AE +A+
Sbjct: 697 IKLAGAVLELGKIEEDICNAVNKLQETEGQISTKEKEVAMARFQRLLLIAGAAKAERSAS 756

Query: 748 LESGDTGEANLLLAEAEAARCEAKKLQPIYNFHEDELSTIPKHFISAELVFNLGREKLAD 807
           LE GD  EA++LLAEAEAA  EA+K+QP+YNF E+E + +PK +IS ELV NLG ++LA+
Sbjct: 757 LELGDLDEADILLAEAEAAESEARKIQPVYNFKEEEFANLPKQYISMELVSNLGGKQLAE 816

Query: 808 LVAS 812
           L AS
Sbjct: 817 LAAS 820

BLAST of Carg21335 vs. TrEMBL
Match: tr|A0A1U7Y309|A0A1U7Y309_NICSY (LOW QUALITY PROTEIN: spindle pole body component 110 OS=Nicotiana sylvestris OX=4096 GN=LOC104243932 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.0e-121
Identity = 330/815 (40.49%), Postives = 453/815 (55.58%), Query Frame = 0

Query: 10  EDENEND---SLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNSTADKVTT 69
           ED +E D   SLFEGMVLF+PS                    D+      L++ AD    
Sbjct: 7   EDSSEKDEMVSLFEGMVLFNPS-------------------ADVGQ----LSTVAD--AD 66

Query: 70  TASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQNSLQIT 129
           T+++S S+++ +P  S   +EPLDE+LFSDLTL+T                         
Sbjct: 67  TSASSPSTSMQLPAVS---NEPLDENLFSDLTLMT------------------------- 126

Query: 130 DPSQVATITNTPASAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAXXXXXXXXXX 189
            PSQ                                    GLRIGYGRD           
Sbjct: 127 -PSQ---SLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGLRIGYGRDRDYASDDRPQS 186

Query: 190 XXXXXXXXXXXXXXXXXXXXLSKIQ----------PSASPPQTLSSHSSSVDKMESPNFV 249
                               L +            PS+ P  T    S+  D   + N  
Sbjct: 187 HPLPNIHLNEIGSASHEEQKLEEEAEEWRQQENNIPSSIPAAT-EQDSNGTDSDRNGNLA 246

Query: 250 SQEDVIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASR 309
           S   +        DA+ L   +      +  E K EQ+R  I++ L  AR +V SVSA R
Sbjct: 247 SVTPI----SENMDASHLNAKA------DPVELKFEQIRASIADKLKLAREAVVSVSAMR 306

Query: 310 KDVIQRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNE 369
           K+ I+RRRK +D+   +  ++  LE++L+EACEAEDFETAER+SE+LA AE EK   +  
Sbjct: 307 KESIRRRRKAVDDFNGASSRHRELEQKLDEACEAEDFETAERVSENLALAEKEKDRLVIL 366

Query: 370 LKDVEALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELE 429
           LKD E  CD +D++M EVLD LI  EE+C  LLR F+ +AA++    L  AEA S+K+  
Sbjct: 367 LKDAETHCDVVDTKMQEVLDSLIQAEEECVYLLRRFSEEAASNADSVLMNAEAMSSKDNA 426

Query: 430 KWHLSNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELE 489
           +W  S E +E +K E EI+S ++ E+  +LN+S++  VEDD+RE +VLC RK  L +EL 
Sbjct: 427 EWLSSVEAIEVRKFELEIQSQLVHEAKSILNESIDQSVEDDQREIDVLCMRKEELAEELR 486

Query: 490 KLLALVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQ 549
           KLLALV+EK+ EI END+LI  VE RI   +S F+ V +++D  YD LQS LS+L +E++
Sbjct: 487 KLLALVKEKEAEIAENDALIQKVENRIDGVVSCFKEVQASIDNNYDKLQSCLSELMVENE 546

Query: 550 SLSVKRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKAS 609
            L  K+++ID +L+ E+ +GE++K +++ S ++A  Y E+A LRK L   I K +  + +
Sbjct: 547 VLLRKKKDIDNYLSQEESRGEKIKNLSRSSADEANMYEEVAGLRKSLSMFISKSKAYRVN 606

Query: 610 LSQSEDKLSKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAE 669
           L++SE+++S+DVQ+L                            KQR+ FID+R+PELEAE
Sbjct: 607 LAKSEERISEDVQILKQETFAARSSLQELSSTKSSIQQEVESCKQRLLFIDRRIPELEAE 666

Query: 670 KKVAAAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEV 729
           KKVAA  RNFKEAAR+AAEAK+L  EK+ I   +D   L+L+KLEEK   T   LQETEV
Sbjct: 667 KKVAATTRNFKEAARLAAEAKALCIEKEEIQCKMDSTKLELKKLEEKICHTFDRLQETEV 726

Query: 730 SIQSKEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPI 789
            I  KEKE+A  RFQRL+LIA A  AE +AALE G   EA  L+AEA+AA  EA+KLQPI
Sbjct: 727 XISLKEKELAMTRFQRLILIARATRAERSAALELGHQEEAEALMAEADAAEVEARKLQPI 753

Query: 790 YNFHEDELSTIPKHFISAELVFNLGREKLADLVAS 812
           YNF E+E+  +PK FIS+ELV  LG ++LA+L AS
Sbjct: 787 YNFKEEEIEDLPKLFISSELVSMLGGKQLAELAAS 753

BLAST of Carg21335 vs. TrEMBL
Match: tr|A0A1J6JX45|A0A1J6JX45_NICAT (Uncharacterized protein OS=Nicotiana attenuata OX=49451 GN=A4A49_39060 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 3.0e-121
Identity = 339/815 (41.60%), Postives = 459/815 (56.32%), Query Frame = 0

Query: 10  EDENEND---SLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNSTADKVTT 69
           ED ++ D   SLFEGMVLF+P    +  L T  D D+    P IS Q             
Sbjct: 33  EDSSKKDEMGSLFEGMVLFNPGA-DVGQLSTVADADASASSPSISMQ------------- 92

Query: 70  TASNSASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQNSLQIT 129
                      +P  S   +EPLDE+LFSDLTL+T S              L+       
Sbjct: 93  -----------LPAVS---NEPLDENLFSDLTLMTPSQ----------SLLLEXXXXXXQ 152

Query: 130 DPSQVATITNTPASAVGEATERDRGVVSISRQIS-RRKRRPGLRIGYGRD---------A 189
            PS +                      ++SRQ S    ++ GLRIGYGRD          
Sbjct: 153 SPSDLP---------------------ALSRQSSXXXXKKAGLRIGYGRDRDYASDNRPQ 212

Query: 190 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQPSASPPQTLSSHSSSVDKMESPNFV 249
                            XXXXXXXXXXXXX                 S+  D   + N  
Sbjct: 213 SHPLPHTHLNEIVSASHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDSNGTDSDRNGNLA 272

Query: 250 SQEDVIMGSPNQQDANRLEKNSVETEVCNSPEFKLEQVRIQISENLTSARNSVASVSASR 309
           S   +        DA+ L   +      +  E K EQ+R  I++ L  AR +V SVSA R
Sbjct: 273 SATPI----SENMDASHLNAKA------HPVELKFEQIRASIADKLKLAREAVTSVSAMR 332

Query: 310 KDVIQRRRKIMDNLKISLDKYSNLERQLEEACEAEDFETAERLSESLASAEGEKQAFLNE 369
           K+ I RRRK +D+   +  ++  LE++L+EACEAEDFETAER+SE+LA  E EK   +  
Sbjct: 333 KESIHRRRKAVDDFNGASSRHRELEKKLDEACEAEDFETAERVSENLALVEKEKDRLVIL 392

Query: 370 LKDVEALCDAMDSRMHEVLDFLIATEEKCDSLLRTFATDAANDVVLGLNTAEAESAKELE 429
           L+D E  CD +D++M EVLD LI  EE+C  LLR F+ +AA+     L  AE  S+K+  
Sbjct: 393 LRDAETYCDVVDTKMQEVLDSLIQAEEECVYLLRRFSEEAASSADSVLMNAETMSSKDSA 452

Query: 430 KWHLSNEVLEAKKMETEIESLIIQESCMVLNDSVELLVEDDKREKNVLCQRKAVLTDELE 489
           +W  S E +E  K E EI+S ++ E+  +LN+S++  VEDD+RE +VLC RK  L +EL 
Sbjct: 453 EWLSSVEAIEVSKFELEIQSQLVHEAKSILNESIDQSVEDDQREIDVLCMRKEELAEELR 512

Query: 490 KLLALVEEKKREIEENDSLIDAVEKRISVAISGFQHVHSNMDAKYDSLQSTLSQLQLESQ 549
           KLLALV+EK+ EI END+LI  VE RI   +S F+ V +++D  YD LQS LS+L +E++
Sbjct: 513 KLLALVKEKEAEIAENDALIQKVENRIDGVVSCFKEVQASIDNNYDKLQSCLSELMVENE 572

Query: 550 SLSVKRREIDEFLNLEKDKGEQLKKIAQLSIEDAEAYREIARLRKFLMSNILKIREDKAS 609
            L  K+++ID +L+ E+ +GE++K +++ + ++A  Y E+A LRK L   I K +  + +
Sbjct: 573 VLLRKKKDIDNYLSQEESRGEKIKNLSRSAADEANMYEEVAGLRKSLSMFISKSKAYRVN 632

Query: 610 LSQSEDKLSKDVQMLXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQRISFIDKRVPELEAE 669
           L++SE+++S+DVQ+L                            KQR+ FID+R+PELEAE
Sbjct: 633 LAKSEERISEDVQILKQETFAARSSLQELSSTKSSIQQEVESCKQRLLFIDRRIPELEAE 692

Query: 670 KKVAAAGRNFKEAARVAAEAKSLSNEKDSICIDIDRALLDLEKLEEKTRDTMKWLQETEV 729
           KKVAA  RNFKEAAR+AAEAK+L  EK+ I    D   L+L+KLEEK   T+  LQETEV
Sbjct: 693 KKVAATTRNFKEAARLAAEAKALCIEKEEIQCKKDSTKLELKKLEEKICHTVDRLQETEV 752

Query: 730 SIQSKEKEVAKARFQRLLLIAGAAAAEGAAALESGDTGEANLLLAEAEAARCEAKKLQPI 789
            I SKEKE+A  RFQRL+LIA AA AE +AALE G   EA  L+AEA+AA  EA+KLQPI
Sbjct: 753 QISSKEKELAMIRFQRLILIARAARAERSAALELGHQEEAEALMAEADAAEVEARKLQPI 778

Query: 790 YNFHEDELSTIPKHFISAELVFNLGREKLADLVAS 812
           YNF E+E+  +PK  IS+ELV  LG ++LA+L AS
Sbjct: 813 YNFKEEEIEDLPKLLISSELVSMLGGKQLAELAAS 778

BLAST of Carg21335 vs. TrEMBL
Match: tr|A0A1U8ATW4|A0A1U8ATW4_NELNU (calponin homology domain-containing protein DDB_G0272472 OS=Nelumbo nucifera OX=4432 GN=LOC104603853 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 2.2e-119
Identity = 336/847 (39.67%), Postives = 459/847 (54.19%), Query Frame = 0

Query: 12  ENENDSLFEGMVLFDPSEYPIQILPTPEDQDSDHPGPDISNQPHPLNSTADKVTTTASNS 71
           E E  SLFEGMVLF+PS+       TP+D   D                           
Sbjct: 4   EEEMGSLFEGMVLFNPSQI------TPDDDSED--------------------------- 63

Query: 72  ASSTVTMPTSSSHLSEPLDEDLFSDLTLVTTSSMHKGQAKHQTQFQLDQNSLQITDPSQV 131
             +  + P++SS  S PLDE+LFSDLT+V                      L   +P  +
Sbjct: 64  -KTPSSFPSTSSSTSLPLDENLFSDLTVVPL-----------------LQPLTSQEPPNI 123

Query: 132 ATIT------------NTPASAVGEATERDRGVVSISRQISRRKRRPGLRIGYGRDAXXX 191
           + +T                            + ++SRQISR+K+R G+RIGY RD    
Sbjct: 124 SPLTXXXXXXXXXXXXXXXXXXXXXXXXXXXXIPTVSRQISRKKKRAGIRIGYARDTQVS 183

Query: 192 XXXXXXXXXXXXXXXXXXXXXXXXXXXLSKIQP---SASPPQTLSSHSSSVDKMESPNFV 251
                         XXXXXXXXXXXXX     P    AS P     + S+V+   S +  
Sbjct: 184 DDHHNYPSFSLTESXXXXXXXXXXXXXXXXXGPVDLRASKPVISEEYISTVEDHLSFS-A 243

Query: 252 SQEDVIMGSPNQQ-----DANRLEKNSVETEVCNSP------------------------ 311
            Q   ++   N +         +  N+ + E   SP                        
Sbjct: 244 EQSSCLLSDSNSELSPGVTVTEISYNNSKDESSCSPVRASNQDLRKDNELSLQSKQEAGV 303

Query: 312 --EFKLEQVRIQISENLTSARNSVASVSASRKDVIQRRRKIMDNLKISLDKYSNLERQLE 371
             E K E++R QISE L   R   + V A+RK+V +RRRK  +++ +   KY  LER+LE
Sbjct: 304 LTEEKWEKIRAQISEKLEQTRKLASFVLATRKEVARRRRKASEDVNMVSVKYKQLERELE 363

Query: 372 EACEAEDFETAERLSESLASAEGEKQAFLNELKDVEALCDAMDSRMHEVLDFLIATEEKC 431
           EACEAEDFE AER+SE                   EA CD ++ +M EVL+  I  EE+ 
Sbjct: 364 EACEAEDFERAERVSEXXXXXXXXXXXXXXXXXXAEADCDLVELKMQEVLELQITAEEEG 423

Query: 432 DSLLRTFATDAANDVVLGLNTAEAESAKELEKWHLSNEVLEAKKMETEIESLIIQESCMV 491
            SLL  FA DAA +  L L  AE+   KE+ +W  S EVLE KKME EIES +I + C+ 
Sbjct: 424 VSLLERFAEDAARNADLILKNAESLHLKEMNEWQSSTEVLEVKKMELEIESQLISDVCLG 483

Query: 492 LNDSVELLVEDDKREKNVLCQRKAVLTDELEKLLALVEEKKREIEENDSLIDAVEKRISV 551
           LNDS+E L++DD+ EK  LC+++ +LT+ELE LL LV +K+ EI +N+S I AVEKRI  
Sbjct: 484 LNDSIEQLIKDDRIEKEFLCKKRDILTEELENLLDLVRQKEAEIADNNSNIQAVEKRIEN 543

Query: 552 AISGFQHVHSNMDAKYDSLQSTLSQLQLESQSLSVKRREIDEFLNLEKDKGEQLKKIAQL 611
            +SGF+   S++D KY+SL+S LS+++ E+++LS+K+++ D  L+ E+  G +L+++A +
Sbjct: 544 TVSGFRDAQSSIDMKYNSLKSALSEVESENEALSMKKKQADCILSQEEKIGGKLRELASV 603

Query: 612 SIEDAEAYREIARLRKFLMSNILKIREDKASLSQSEDKLSKDVQMLXXXXXXXXXXXXXX 671
           S+++A+  +E+  LRK L  +ILK REDK  L+++E+K+ +DVQ L              
Sbjct: 604 SMDEAKTCQELLGLRKSLALSILKCREDKVRLAKTEEKILEDVQNLRQEISAARAFLQEL 663

Query: 672 XXXXXXXXXXXXXXKQRISFIDKRVPELEAEKKVAAAGRNFKEAARVAAEAKSLSNEKDS 731
                         KQR+ FIDKR PEL+AEKKVAA  RNFKEA R+AAEAK+L+ EK+ 
Sbjct: 664 SSTKSNIQQEIASFKQRLFFIDKRGPELDAEKKVAATARNFKEAGRLAAEAKALTVEKEG 723

Query: 732 ICIDIDRALLDLEKLEEKTRDTMKWLQETEVSIQSKEKEVAKARFQRLLLIAGAAAAEGA 791
           I   +  A L+LEK+EE+   T   LQETE  I SKEKE A AR  RL L+AGAA AE +
Sbjct: 724 IQAKMQTATLELEKIEEEVNCTATGLQETEALILSKEKEAAMARCGRLRLVAGAAMAESS 783

Query: 792 AALESGDTGEANLLLAEAEAARCEAKKLQPIYNFHEDELSTIPKHFISAELVFNLGREKL 813
            A+E GD  EAN+L  EAEAA CEA KLQ  Y+F EDE   +PKHFIS  LV NLG ++L
Sbjct: 784 GAIELGDLEEANILTTEAEAADCEAIKLQQAYDFKEDEFGNLPKHFISMVLVTNLGGKEL 798