Carg21070 (gene) Silver-seed gourd

NameCarg21070
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRBR-type E3 ubiquitin transferase
LocationCucurbita_argyrosperma_scaffold_122 : 778203 .. 781414 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAACAACGATGGCGGATACTGTAGACGACGATTACCTTCAAGACTTGATCACCGAGCAACGTTCCGAAATCCTTGCCGCCAAAACCCTCATCTCCGACCTCGATTTGGCCTACCAACTTCAAATGCAGGAGGCAATGAACGCCTCTCTTGGTTCGAAATCCTCGTCCAAACGACCTGCAACCGTTAACAGGGTCGTCCCTGTCTTGGAGTCTGAAGAAACTGGCGCGTTGGACTTCGCGACCACGCTTTTGCTTGAGGACATTGCAAGGATTGCCATGGAGCACAAGGACCGGGAGCAGTGTCAGGCGGAGATGAGGAAAATGAAGGAGGAGCTTGACCGTCGGATTCACGATCAGAACTTTGCGAATTACATTCTGAATGTTCCCGATGATGAGTGGAGTAAGTATGGGGATAATTATGAGAAGCCGTATGGTGAGAGTTATTCGTCGTCGTCGTCGTCGTCTTCTTCTTCTTCGTCTGTTGCTTCGTCGAGGGGTTTGTTGGATTCTGAATGTTTTAGGGTGTATTCGAAGGGGCTGATCAGTGAAGAGAGAATTAGAGATATGACGGTTAGGGTTGCCGGTATTGGGGTTGCGGTTTGTGACCCTAAGGATAATCTGTTGTTTGAGAAGACGAGGCCTCTGGAATCTGTGGTGGATGGTAAGGAGACCAGCTGCGAATGCGCTGAGCTTGAAGCGCTGGTTGAAGGGCTTCATATTGCTCTTGTTTTAGGCTTGAAAAAGGTCACTTTTTTCTGTGCAGATTACATGCTCTACCAGTATGTAAGTTCCTACACTTTTCATTATGTCTATACTGTTCTGTGTTTAGAGTTCATTGAATTTTGTCTTTTAATATAATTCTCTGTTGGAAACTACTGATTACCTTTAGAATGCTAAGTGTATGAGCATGAGACTATACTTGCTTTATAATCCCATGATAAGATGTTTTATTGTCAACAAGAAAACTCTTTGAGAAGATTAATCAAATAACAATTAAAATGTCATTGATTATAGGATAACTTGATTACCGATCATTACTTGTGTGCATCTCACATCTCACATGTTTCTTGAGATTTCCCTACAAGTCGTCCTCCCAATCCAAGAGTCTTTGGAGCTTAAAGAAGTGTCTAGTGATGATCCTAAGATATTTAGACCTTCTACTAAGATTCGATATCATCCTACGTGTTAACTTGATTATCGGATAACTTGATTACCAATAATCGTAGGGTTAACTGTGAGAATAATTGAGGTAGGTGCAAACAGGACACTCACAGATATTGAAAACAAACAAGCAAAGTATGGGGGAGTAATTGGATTGAGTGCTTAAATAAAGAGCTTTCTTTGGAAGCCATAATAACGATACAATCTGCGAGGTTGAAATTTACCTTACAGACTAACAAGGTGGTGCGATGGTCATTCTAGCTAGATCATTTTGTTCTAGGATTTTTCATCTCAATTTGTATTTGACTTTATTTGACTTTCTTTTTTGGATTTTTACTGGTGGTATTGGTTTCTTACTCAAGGTTGGTATGAACTTTTTCTCTGTTGCATCATCATCTCCATTGTTCTTAGTGGTTGGCTGAATAATAATGACCGAGACTAGTTTTAAGTAATATGTTTCTAGCTTAATGTTAATATGCTATTTATCATGATTTGAATAGCCAGCTTTTCACTCATTCTTGTTGACAAGTAAAGAAAAAAAATTCCCTATGGCCTTTTCCTTTGAATGATTTATCTTGATTCACATCAGTAATACTGGTGACCTTTCTCATTTTCCGTATCCGATATGAAGAATTCATGTAAGAAAGTGTTGTGATTTAACATCATAGTTCATATTCTGTGGTTACAGATTATACGTAGCGTGGCTCCAGGCTCGAGCTCCATGGCAACATTGGTTAATGAGGTGTCTCTTCTTCAGGGAAAATTTACCTACTGTAACCCGTCCCTCGTGACACGTAATGATATCAAGTTTGCTTTTAGACTTGCAAGAGAGGCTATAGTTTCTCAGATCACCTGGCCTGCAGAATCTGAAAATGGAAAGTGCTTGAAGGAAACTTGTACCATTTGCTTTGAAGATGTTTGTGTGGATCAGATGTTCTCAGTTGATGGTTGTTTGCACAGATACTGCTTTTCTTGTATGAAGCAGGATTTAGAAATAAGGTTGCTCAATGGGAATGGGATGCAGGCAAACTGTCCTCATCTGGGTTGTACGTCGGAAATTAATATCGAAAGCTGTGGAAAGTTCTTGGATAGTAGGGTGATTGAGATTATCAGCCAAAGGATTAAGGAAGCGTCTATGCCCGTTCTGGAGAAAGTTTACTGCCCCTATTCCAGGTGCTCGGCATTAATGTCCAAAAGCGAGCTTTTAAAATATAGCGAGGCTTCATATTTCGATGCAGAACGAACTGGGGCAAGGAAGTGCATGAAATGCAATCTGTTTTTCTGTATTAATTGCAAGGCCCCTTGGCATTATAATATGACTTGCTACGACTTCAGAAAGTTAAATCCCGTTCTCCGTCCTGATGAGGAAATGCTGAAGTCTCTTGCTTCGAAGAAACTTTGGCGCCAATGTGTGACGTGTAACACGATGGTCGAATTGGCCGAGGGCTGCTACCACATCACCTGCAGGTACATTATTTACCTTTATCCATCTCGCTTGCTCCTTTTCTCCCTTGATGAATATGACAGTTCAAAATTCAATATTTCCTTAGCACCCAATGAGCATTAATGCAATTCACTAATGTCTACTTGCTTGGAAGATGGAGTGAGGGTAACAGCATCTGAAAATTGATACATTTTCTTAGAGTAATTATAAAACGAATTCTGAAAAACACCCCTTCGAGTTGGTACTTCGACGAGTATTGTACTTTACTGGATACTTGGTATTGATTTGTCTTGTCAAATAAATGCTAGTAATTGACATTGATTTGTTGAGGTCAGCTCAGTTTTAGAGAGAATTTTATCCGAGTTCCATGAAAGCAAAGACGAGACGATCGACTCGTGATGTTGCTACAAGTTCTTTGTTAAAGTTTTGCTATACATTGGAGTTTTCTTTCCCTACTTTGTAAATATAAATGGATTCTGTGTGCAGATGCGGATATGAGTTTTGCTATACATGTGGAGCCCCCTGGAAAGGTAAGAAGCCTACATGCACCTGTCCAATATGGGACGAGCGGAACATCATACGCAATCAACGTCAACGTCGACGATAA

mRNA sequence

GCAACAACGATGGCGGATACTGTAGACGACGATTACCTTCAAGACTTGATCACCGAGCAACGTTCCGAAATCCTTGCCGCCAAAACCCTCATCTCCGACCTCGATTTGGCCTACCAACTTCAAATGCAGGAGGCAATGAACGCCTCTCTTGGTTCGAAATCCTCGTCCAAACGACCTGCAACCGTTAACAGGGTCGTCCCTGTCTTGGAGTCTGAAGAAACTGGCGCGTTGGACTTCGCGACCACGCTTTTGCTTGAGGACATTGCAAGGATTGCCATGGAGCACAAGGACCGGGAGCAGTGTCAGGCGGAGATGAGGAAAATGAAGGAGGAGCTTGACCGTCGGATTCACGATCAGAACTTTGCGAATTACATTCTGAATGTTCCCGATGATGAGTGGAGTAAGTATGGGGATAATTATGAGAAGCCGTATGGTGAGAGTTATTCGTCGTCGTCGTCGTCGTCTTCTTCTTCTTCGTCTGTTGCTTCGTCGAGGGGTTTGTTGGATTCTGAATGTTTTAGGGTGTATTCGAAGGGGCTGATCAGTGAAGAGAGAATTAGAGATATGACGGTTAGGGTTGCCGGTATTGGGGTTGCGGTTTGTGACCCTAAGGATAATCTGTTGTTTGAGAAGACGAGGCCTCTGGAATCTGTGGTGGATGGTAAGGAGACCAGCTGCGAATGCGCTGAGCTTGAAGCGCTGGTTGAAGGGCTTCATATTGCTCTTGTTTTAGGCTTGAAAAAGGTCACTTTTTTCTGTGCAGATTACATGCTCTACCAGTATATTATACGTAGCGTGGCTCCAGGCTCGAGCTCCATGGCAACATTGGTTAATGAGGTGTCTCTTCTTCAGGGAAAATTTACCTACTGTAACCCGTCCCTCGTGACACGTAATGATATCAAGTTTGCTTTTAGACTTGCAAGAGAGGCTATAGTTTCTCAGATCACCTGGCCTGCAGAATCTGAAAATGGAAAGTGCTTGAAGGAAACTTGTACCATTTGCTTTGAAGATGTTTGTGTGGATCAGATGTTCTCAGTTGATGGTTGTTTGCACAGATACTGCTTTTCTTGTATGAAGCAGGATTTAGAAATAAGGTTGCTCAATGGGAATGGGATGCAGGCAAACTGTCCTCATCTGGGTTGTACGTCGGAAATTAATATCGAAAGCTGTGGAAAGTTCTTGGATAGTAGGGTGATTGAGATTATCAGCCAAAGGATTAAGGAAGCGTCTATGCCCGTTCTGGAGAAAGTTTACTGCCCCTATTCCAGGTGCTCGGCATTAATGTCCAAAAGCGAGCTTTTAAAATATAGCGAGGCTTCATATTTCGATGCAGAACGAACTGGGGCAAGGAAGTGCATGAAATGCAATCTGTTTTTCTGTATTAATTGCAAGGCCCCTTGGCATTATAATATGACTTGCTACGACTTCAGAAAGTTAAATCCCGTTCTCCGTCCTGATGAGGAAATGCTGAAGTCTCTTGCTTCGAAGAAACTTTGGCGCCAATGTGTGACGTGTAACACGATGGTCGAATTGGCCGAGGGCTGCTACCACATCACCTGCAGATGCGGATATGAGTTTTGCTATACATGTGGAGCCCCCTGGAAAGGTAAGAAGCCTACATGCACCTGTCCAATATGGGACGAGCGGAACATCATACGCAATCAACGTCAACGTCGACGATAA

Coding sequence (CDS)

GCAACAACGATGGCGGATACTGTAGACGACGATTACCTTCAAGACTTGATCACCGAGCAACGTTCCGAAATCCTTGCCGCCAAAACCCTCATCTCCGACCTCGATTTGGCCTACCAACTTCAAATGCAGGAGGCAATGAACGCCTCTCTTGGTTCGAAATCCTCGTCCAAACGACCTGCAACCGTTAACAGGGTCGTCCCTGTCTTGGAGTCTGAAGAAACTGGCGCGTTGGACTTCGCGACCACGCTTTTGCTTGAGGACATTGCAAGGATTGCCATGGAGCACAAGGACCGGGAGCAGTGTCAGGCGGAGATGAGGAAAATGAAGGAGGAGCTTGACCGTCGGATTCACGATCAGAACTTTGCGAATTACATTCTGAATGTTCCCGATGATGAGTGGAGTAAGTATGGGGATAATTATGAGAAGCCGTATGGTGAGAGTTATTCGTCGTCGTCGTCGTCGTCTTCTTCTTCTTCGTCTGTTGCTTCGTCGAGGGGTTTGTTGGATTCTGAATGTTTTAGGGTGTATTCGAAGGGGCTGATCAGTGAAGAGAGAATTAGAGATATGACGGTTAGGGTTGCCGGTATTGGGGTTGCGGTTTGTGACCCTAAGGATAATCTGTTGTTTGAGAAGACGAGGCCTCTGGAATCTGTGGTGGATGGTAAGGAGACCAGCTGCGAATGCGCTGAGCTTGAAGCGCTGGTTGAAGGGCTTCATATTGCTCTTGTTTTAGGCTTGAAAAAGGTCACTTTTTTCTGTGCAGATTACATGCTCTACCAGTATATTATACGTAGCGTGGCTCCAGGCTCGAGCTCCATGGCAACATTGGTTAATGAGGTGTCTCTTCTTCAGGGAAAATTTACCTACTGTAACCCGTCCCTCGTGACACGTAATGATATCAAGTTTGCTTTTAGACTTGCAAGAGAGGCTATAGTTTCTCAGATCACCTGGCCTGCAGAATCTGAAAATGGAAAGTGCTTGAAGGAAACTTGTACCATTTGCTTTGAAGATGTTTGTGTGGATCAGATGTTCTCAGTTGATGGTTGTTTGCACAGATACTGCTTTTCTTGTATGAAGCAGGATTTAGAAATAAGGTTGCTCAATGGGAATGGGATGCAGGCAAACTGTCCTCATCTGGGTTGTACGTCGGAAATTAATATCGAAAGCTGTGGAAAGTTCTTGGATAGTAGGGTGATTGAGATTATCAGCCAAAGGATTAAGGAAGCGTCTATGCCCGTTCTGGAGAAAGTTTACTGCCCCTATTCCAGGTGCTCGGCATTAATGTCCAAAAGCGAGCTTTTAAAATATAGCGAGGCTTCATATTTCGATGCAGAACGAACTGGGGCAAGGAAGTGCATGAAATGCAATCTGTTTTTCTGTATTAATTGCAAGGCCCCTTGGCATTATAATATGACTTGCTACGACTTCAGAAAGTTAAATCCCGTTCTCCGTCCTGATGAGGAAATGCTGAAGTCTCTTGCTTCGAAGAAACTTTGGCGCCAATGTGTGACGTGTAACACGATGGTCGAATTGGCCGAGGGCTGCTACCACATCACCTGCAGATGCGGATATGAGTTTTGCTATACATGTGGAGCCCCCTGGAAAGGTAAGAAGCCTACATGCACCTGTCCAATATGGGACGAGCGGAACATCATACGCAATCAACGTCAACGTCGACGATAA

Protein sequence

ATTMADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVNRVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFANYILNVPDDEWSKYGDNYEKPYGESYSSSSSSSSSSSSVASSRGLLDSECFRVYSKGLISEERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALVLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFAFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTCPIWDERNIIRNQRQRRR
BLAST of Carg21070 vs. NCBI nr
Match: XP_022964983.1 (uncharacterized protein LOC111464930 [Cucurbita moschata])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 556/557 (99.82%), Postives = 556/557 (99.82%), Query Frame = 0

Query: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVN 63
           MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSK PATVN
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKGPATVN 60

Query: 64  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 123
           RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 120

Query: 124 YILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISE 183
           YILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISE 180

Query: 184 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 243
           ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 240

Query: 244 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 303
           LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 304 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 363
           FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 364 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 423
           IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 420

Query: 424 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 483
           CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 484 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 543
           RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC
Sbjct: 481 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 544 PIWDERNIIRNQRQRRR 561
           PIWDERNIIRNQRQRRR
Sbjct: 541 PIWDERNIIRNQRQRRR 557

BLAST of Carg21070 vs. NCBI nr
Match: XP_023519052.1 (uncharacterized protein LOC111782521 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1071.2 bits (2769), Expect = 1.2e-309
Identity = 548/557 (98.38%), Postives = 555/557 (99.64%), Query Frame = 0

Query: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVN 63
           MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAM+ASLGSKSSSKRPA+VN
Sbjct: 1   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMDASLGSKSSSKRPASVN 60

Query: 64  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 123
           RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEE+DRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEEIDRRIHDQNFAN 120

Query: 124 YILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISE 183
           YILNVPDDEWSKYGDNY XXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISE
Sbjct: 121 YILNVPDDEWSKYGDNYEXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISE 180

Query: 184 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 243
           ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGL+IALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLNIALV 240

Query: 244 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 303
           LGLKKVTFFCADYMLYQYIIRSVAPGSSSMAT+VNEVSLLQGKFTYCNPSLVTRNDIKFA
Sbjct: 241 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATVVNEVSLLQGKFTYCNPSLVTRNDIKFA 300

Query: 304 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 363
           FRLAREAIVSQITWPAESENGKCLKETCTICFED+CVDQMFSVDGCLHRYCFSCMKQDLE
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDICVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 364 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 423
           IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVI+IISQRIKEASMPVLEKVYCPYSR
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIDIISQRIKEASMPVLEKVYCPYSR 420

Query: 424 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 483
           CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL
Sbjct: 421 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 480

Query: 484 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 543
           RPDEEMLKSLASKKLWRQCV CNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC
Sbjct: 481 RPDEEMLKSLASKKLWRQCVACNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 540

Query: 544 PIWDERNIIRNQRQRRR 561
           PIWDERNIIRNQRQRRR
Sbjct: 541 PIWDERNIIRNQRQRRR 557

BLAST of Carg21070 vs. NCBI nr
Match: XP_022970417.1 (uncharacterized protein LOC111469406 [Cucurbita maxima])

HSP 1 Score: 1039.6 bits (2687), Expect = 3.7e-300
Identity = 535/557 (96.05%), Postives = 547/557 (98.20%), Query Frame = 0

Query: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVN 63
           MADTVDD+YL DLITEQRSEILAAKTLISDLD AYQLQMQEAMNASLGSKSSSKRPATVN
Sbjct: 1   MADTVDDNYLLDLITEQRSEILAAKTLISDLDFAYQLQMQEAMNASLGSKSSSKRPATVN 60

Query: 64  RVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFAN 123
           RVVPVLESEETGALDFATTLLLEDIARIAMEH DREQCQAEMRKMKEELDRRIHDQNFAN
Sbjct: 61  RVVPVLESEETGALDFATTLLLEDIARIAMEHTDREQCQAEMRKMKEELDRRIHDQNFAN 120

Query: 124 YILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISE 183
           Y+LNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLIS+
Sbjct: 121 YLLNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLISQ 180

Query: 184 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIALV 243
           ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGL+IALV
Sbjct: 181 ERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLNIALV 240

Query: 244 LGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKFA 303
           LGLKKVTFFCADY LYQYIIRSVAPGSSSMATLVNEVSLLQGKFT CNPSL+TRNDIKFA
Sbjct: 241 LGLKKVTFFCADYRLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTCCNPSLLTRNDIKFA 300

Query: 304 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 363
           FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE
Sbjct: 301 FRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLE 360

Query: 364 IRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSR 423
           IRLLNGNGMQANCPHLGCTSEINI+SCGKFLDSRVIEIISQRIKEAS+PVLEKVYCPYSR
Sbjct: 361 IRLLNGNGMQANCPHLGCTSEINIKSCGKFLDSRVIEIISQRIKEASVPVLEKVYCPYSR 420

Query: 424 CSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVL 483
           CS LMSKSELLKYSEAS+F+A+ TGARKCMKCNLFFCINCK PWHY+MTCY+FRKLNPVL
Sbjct: 421 CSTLMSKSELLKYSEASFFNAKLTGARKCMKCNLFFCINCKVPWHYDMTCYNFRKLNPVL 480

Query: 484 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCTC 543
           RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWK KKPTCTC
Sbjct: 481 RPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCTC 540

Query: 544 PIWDERNIIRNQRQRRR 561
           PIWDERNII NQRQRRR
Sbjct: 541 PIWDERNIICNQRQRRR 557

BLAST of Carg21070 vs. NCBI nr
Match: XP_022157931.1 (uncharacterized protein LOC111024540 [Momordica charantia])

HSP 1 Score: 878.6 bits (2269), Expect = 1.1e-251
Identity = 447/560 (79.82%), Postives = 491/560 (87.68%), Query Frame = 0

Query: 4   MADTV-DDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATV 63
           MADT+ DDDYLQ++++EQRSE+LAAKTL SDLDLA+QLQ+QEAMNASL SK S++   + 
Sbjct: 1   MADTMDDDDYLQEVVSEQRSELLAAKTLFSDLDLAFQLQLQEAMNASLDSKPSTQPSPSR 60

Query: 64  NRVVPVLESEE-TGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNF 123
           NRVVP LESE+ + ALD ATTL+LEDIAR AME KDREQC  EMRKMKEELDRRIHDQNF
Sbjct: 61  NRVVPKLESEDSSSALDLATTLMLEDIARFAMEFKDREQCHTEMRKMKEELDRRIHDQNF 120

Query: 124 ANYILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLI 183
           A YI NVP+DEW +YGDNY          XXXXXXXXXXXXX R  LDSE FRVYSKGLI
Sbjct: 121 ATYIRNVPEDEWRRYGDNY----EKPYSGXXXXXXXXXXXXXARVCLDSEIFRVYSKGLI 180

Query: 184 SEERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIA 243
           SEERIRDM VRVA IGVAVCDPKDNLLFEK  PLESVVDGKE + ECAELEALVEGL++A
Sbjct: 181 SEERIRDMKVRVAAIGVAVCDPKDNLLFEKMMPLESVVDGKEINSECAELEALVEGLNVA 240

Query: 244 LVLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIK 303
           L+LGLK VTFFCADYMLYQY+   V PG+SS+ATLVNEV LLQ +FTYCNPSLVTRND+K
Sbjct: 241 LILGLKNVTFFCADYMLYQYLTGRVPPGASSLATLVNEVILLQRRFTYCNPSLVTRNDMK 300

Query: 304 FAFRLAREAIVSQITWPAESENGKCL-KETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQ 363
           FAF+LAREAIVSQITWP E+ NGKCL KETC ICFEDV VDQMFSVDGCLHRYCFSCMKQ
Sbjct: 301 FAFKLAREAIVSQITWPTEAGNGKCLKKETCIICFEDVRVDQMFSVDGCLHRYCFSCMKQ 360

Query: 364 DLEIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCP 423
            +E+RLLNGNGM+ NCPH GCTS INIESCGKFL+ +VIEI+SQRIKEAS+P+LEKVYCP
Sbjct: 361 HVEVRLLNGNGMKVNCPHQGCTSGINIESCGKFLEPKVIEIMSQRIKEASVPILEKVYCP 420

Query: 424 YSRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLN 483
           YSRCSALMSKSELLKY+EAS+  AE++GARKCMKCN FFCINCK PWHYN+TCYD+RK N
Sbjct: 421 YSRCSALMSKSELLKYTEASFIGAEQSGARKCMKCNQFFCINCKVPWHYNLTCYDYRKSN 480

Query: 484 PVLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPT 543
           P  RPDE MLKSLAS+KLWRQCV CN MVELAEGCYHITCRCGYEFCY CGAPWK KK T
Sbjct: 481 PNPRPDETMLKSLASRKLWRQCVKCNNMVELAEGCYHITCRCGYEFCYICGAPWKNKKGT 540

Query: 544 CTCPIWDERNIIRNQRQRRR 561
           CTCPIWDERNIIR+QRQ+RR
Sbjct: 541 CTCPIWDERNIIRDQRQQRR 556

BLAST of Carg21070 vs. NCBI nr
Match: XP_008463535.1 (PREDICTED: uncharacterized protein LOC103501665 [Cucumis melo])

HSP 1 Score: 867.1 bits (2239), Expect = 3.3e-248
Identity = 424/553 (76.67%), Postives = 477/553 (86.26%), Query Frame = 0

Query: 4   MADTV-DDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATV 63
           MADT+ DDD +Q+LIT+QRSE+L+AKTL SDLD A+QLQ+QEAM+ASL S  S +  +++
Sbjct: 1   MADTLNDDDCVQELITQQRSELLSAKTLFSDLDYAFQLQLQEAMDASLTSIPSIESSSSL 60

Query: 64  NRVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFA 123
           N   PVL+SEET  LD ATTL+LEDIAR AME KDRE CQ EMRKMKEELDRRIHDQ FA
Sbjct: 61  NTADPVLDSEETSGLDLATTLMLEDIARFAMEFKDREHCQTEMRKMKEELDRRIHDQKFA 120

Query: 124 NYILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLIS 183
            YI +VP++EW +YGDNY       XXXXXXXXX           +DSECFRVYSKGLIS
Sbjct: 121 EYIRSVPENEWREYGDNYEKPYGESXXXXXXXXXWN---------VDSECFRVYSKGLIS 180

Query: 184 EERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIAL 243
           EERIRD  VRVAGIG+AVCDPKDNLLFEK  P+ES+V+GKETS ECAELEALVEGL++AL
Sbjct: 181 EERIRDTVVRVAGIGIAVCDPKDNLLFEKMSPIESMVEGKETSNECAELEALVEGLNVAL 240

Query: 244 VLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKF 303
           +LGLK +TFFCADYMLYQY+   V P +SS A LVNEV +LQGKFTYCNPSLVTRNDIKF
Sbjct: 241 ILGLKSITFFCADYMLYQYLTGRVPPATSSTAKLVNEVVVLQGKFTYCNPSLVTRNDIKF 300

Query: 304 AFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDL 363
           AF+LAREAIVSQITWPAE++NG CLKETCTICFEDV VDQMFSVDGCLHRYCFSCMKQ +
Sbjct: 301 AFKLAREAIVSQITWPAEADNGNCLKETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHV 360

Query: 364 EIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYS 423
           E++LLNGNGMQANCPH GCTSE+NIESCGKFL+ +V EI+SQRIKEAS+P+ EKVYCPYS
Sbjct: 361 EVKLLNGNGMQANCPHQGCTSEVNIESCGKFLEPKVFEIMSQRIKEASVPIQEKVYCPYS 420

Query: 424 RCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPV 483
           RCSALMSK+ELL+Y+EASY DAERTGARKCMKCN FFCINCK  WHYN+TCYD+R LNP 
Sbjct: 421 RCSALMSKTELLRYTEASYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDYRNLNPN 480

Query: 484 LRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCT 543
           L PDE+MLKSLA++KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KKPTC 
Sbjct: 481 LHPDEQMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCK 540

Query: 544 CPIWDERNIIRNQ 556
           CPIWDERNII  +
Sbjct: 541 CPIWDERNIIHRR 544

BLAST of Carg21070 vs. TAIR10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 363.6 bits (932), Expect = 2.2e-100
Identity = 175/390 (44.87%), Postives = 255/390 (65.38%), Query Frame = 0

Query: 173 FRVYSKGLISEERIR-DMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAEL 232
           +R+Y KGL+S+E  +  MT  V+G GVA+CD +DNLLFE   PL   +D    S + AEL
Sbjct: 145 YRLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPL---ID-NGMSRQGAEL 204

Query: 233 EALVEGLHIALVLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCN 292
           +AL+ GL  AL LG+K + FFC  Y ++QY+          ++ L++++  +   F+   
Sbjct: 205 KALIRGLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQ 264

Query: 293 PSLVTRNDIKFAFRLAREAIVSQITWPAESENGKC-LKETCTICFEDVCVDQMFSVDGCL 352
             LV RND+KFA++LARE+I+S +T   +    K  LKE C ICF D+  + MFSVD C 
Sbjct: 265 HVLVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCR 324

Query: 353 HRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEAS 412
           HR+CF C+KQ +E++LL  +GM   CPH GC SE+ I++CGK L  ++ ++  QR++E +
Sbjct: 325 HRFCFQCVKQHVEVKLL--HGMAPKCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENA 384

Query: 413 MPVLEKVYCPYSRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYN 472
           +PV E+VYCPY RCSALMSK+++ + +++      ++G R+C++C   FC++CK PWH N
Sbjct: 385 IPVTERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGN 444

Query: 473 MTCYDFRKLNPVLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTC 532
           ++C +++KL+P    D+  LKSLA+ K+WRQC  C  M+EL++GC HITCRCG+EFCY C
Sbjct: 445 LSCTEYKKLHPEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNC 504

Query: 533 GAPWKGKKPTC--TCPIWDERNIIRNQRQR 559
           G  W     TC   CP W+E  I R    R
Sbjct: 505 GGGWNKIMGTCLNRCPTWNEEYITRQDPAR 528

BLAST of Carg21070 vs. TAIR10
Match: AT2G25370.1 (RING/U-box superfamily protein)

HSP 1 Score: 292.7 bits (748), Expect = 4.7e-79
Identity = 156/370 (42.16%), Postives = 220/370 (59.46%), Query Frame = 0

Query: 168 LDSECFRVYSKGLISEERIRDMTVRV--AGIGVAVCDPKDNLLFEKTRPLESVVDGKETS 227
           +D+  +R+  KGL++ E + D   +   AG GVA+CD KDNLL E    L  +    E S
Sbjct: 149 MDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIKESLRDI----EIS 208

Query: 228 CECAELEALVEGLHIALVLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQG 287
               E+ ALV GL  +  LG++ V  +C D  +YQ II         +  LV EV  +  
Sbjct: 209 RRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSII-GRGKSKKKIDHLVEEVQGILE 268

Query: 288 KFTYCNPSLVTRNDIKFAFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFS 347
           K    +  LV RND+KFAFRLAREAI  + +    +E G    ETC I FE+  V+ MF 
Sbjct: 269 KMACIDAVLVARNDVKFAFRLAREAI-GRNSVDVNAEQG----ETCGIFFEETDVEHMFV 328

Query: 348 VDGCLHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQR 407
            + CLHR+CF C+KQ ++++L +G   +  C   GC  ++ +E C K L  ++IE+  Q+
Sbjct: 329 TEKCLHRHCFPCVKQHVKVKLRSGT--EPTCLEYGCKFKLTLERCSKVLTLKLIEMWKQK 388

Query: 408 IKEASMPVLEKVYCPYSRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKA 467
           +KE S+P  E++YCPY  CS LMSK+EL         +A+ +  R C+KC   FCI+CK 
Sbjct: 389 MKEDSIPAAERIYCPYPNCSMLMSKTELSS-------EADLSNVRTCVKCCGLFCIDCKV 448

Query: 468 PWHYNMTCYDFRKLNPVLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYE 527
           P H +++  D++KL+P    D+  LKSLA+ K+WRQCV C  M+EL+ GC H+TCRCGYE
Sbjct: 449 PSHTDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYE 499

Query: 528 FCYTCGAPWK 536
           FCY CG  W+
Sbjct: 509 FCYECGIEWQ 499

BLAST of Carg21070 vs. TAIR10
Match: AT2G19610.2 (RING/U-box superfamily protein)

HSP 1 Score: 288.9 bits (738), Expect = 6.8e-78
Identity = 151/374 (40.37%), Postives = 219/374 (58.56%), Query Frame = 0

Query: 173 FRVYSKGLISEERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELE 232
           +R+YSKGL+SEE I+D T+ V G+G+++CD  D    E  + L +         E AEL 
Sbjct: 50  YRLYSKGLVSEELIKDDTMLVVGLGLSLCDSHDYTKQEINKALRN--QKLAAHPEAAELA 109

Query: 233 ALVEGLHIALVLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNP 292
           A++ GL  AL LG++++ FFC D  +  Y+ R  AP  S +A L+  VSLLQ +FT C  
Sbjct: 110 AIIHGLKWALELGIERIQFFCDDSNILAYVTRKAAPNESIVAKLLEHVSLLQTRFTSCQA 169

Query: 293 -SLVTRNDIKFAFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLH 352
            + V+R+DI    +LA++AI SQ  W      G    E+C +C+  V  +  F V GC H
Sbjct: 170 LATVSRDDIVSVIKLAKDAIASQTRWC----EGDTEYESCPVCYAYVSPNDKFEVQGCFH 229

Query: 353 RYCFSCMKQDLEI-RLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEAS 412
           R C +CM++     ++L GN   A CP+  C +++  E C  F D+  I ++ QR KE +
Sbjct: 230 RICVTCMRKPFSSEQILRGN--TAICPYPDCENDLVPEDCRAFADADAITLMIQRKKEKA 289

Query: 413 MPVLEKVYCPYSRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYN 472
           +PV ++VYCP   CS LMS  +L+++   +   +E   ARKCM+C L FC  C  PWHY 
Sbjct: 290 IPVKDRVYCPNPSCSFLMSDLDLIRHISKNPRHSEE--ARKCMECGLSFCKKCHVPWHYK 349

Query: 473 MTCYDFRKLNPVLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCR-CGYEFCYT 532
            TC +F+K    L+ D  +L+S    + W++C  C ++V+   GC  +TCR C +EFCYT
Sbjct: 350 KTCDEFKKSESYLKSDAAILESFVKTQGWKKCSQCQSIVQHGGGCQQMTCRHCKHEFCYT 409

Query: 533 CGAPWKGKKPTCTC 544
           CGAP K KK TC C
Sbjct: 410 CGAPCKKKKLTCKC 413

BLAST of Carg21070 vs. TAIR10
Match: AT3G45580.1 (RING/U-box protein with C6HC-type zinc finger)

HSP 1 Score: 285.0 bits (728), Expect = 9.8e-77
Identity = 151/373 (40.48%), Postives = 219/373 (58.71%), Query Frame = 0

Query: 171 ECFRVYSKGLISEERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAE 230
           + +R+Y KGL+SEE +      +AG GVA+CD  DNLLF+     E V D + T  E  E
Sbjct: 26  DSYRLYFKGLVSEETVE----LLAGFGVAICDKDDNLLFQMK---EQVHDSRVTVLE-VE 85

Query: 231 LEALVEGLHIALVLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYC 290
           + AL  GL  A+ LG+  ++ +   Y +++ ++   A    + A L++ V  ++ + T  
Sbjct: 86  IMALKRGLTEAVGLGIDNISIYSDHYRIFELVMEKSASAEENFALLMDNVQHIRQRLTSS 145

Query: 291 NPSLVTRNDIKFAFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQ-MFSVDGC 350
            P LVTRN IKF + LA E IVS+I+      +     +TC+IC +D    + MFSV  C
Sbjct: 146 FPVLVTRNQIKFVYELAMETIVSEISIHIPDHD-----KTCSICSDDNFEPELMFSVALC 205

Query: 351 LHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEA 410
            H +C  C+K+ +E+RLL G      C H  C S++ + +C   L S++  +   RI+E 
Sbjct: 206 GHEFCVECVKRHIEVRLLAGG--VPRCLHYQCESKLTLANCANLLTSKLKAMWELRIEEE 265

Query: 411 SMPVLEKVYCPYSRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHY 470
           S+PV E+VYCP  RCS+LMS ++L      S    E    R C+KC   FCINCK PWH 
Sbjct: 266 SIPVEERVYCPNPRCSSLMSVTKL------SNSTREDVTMRSCVKCGEPFCINCKLPWHS 325

Query: 471 NMTCYDFRKLNPVLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYT 530
           N++C D++ L P    D+  LK+LA++K+WRQC  C  ++EL+EGC HITCRCG++FCY 
Sbjct: 326 NLSCNDYKSLGPNPTADDIKLKALANQKMWRQCENCKNVIELSEGCMHITCRCGHQFCYK 377

Query: 531 CGAPWKGKKPTCT 543
           CGA W   +  CT
Sbjct: 386 CGAKWITGRVFCT 377

BLAST of Carg21070 vs. TAIR10
Match: AT5G37560.1 (RING/U-box superfamily protein)

HSP 1 Score: 279.6 bits (714), Expect = 4.1e-75
Identity = 149/383 (38.90%), Postives = 222/383 (57.96%), Query Frame = 0

Query: 168 LDSECFRVYSKGLISEERIRDMTVRV-AGIGVAVCDPKDNLLFEKTRPLESVVDGKETSC 227
           +D+  +++Y KGL+S E   D    V  G GVA+CD  DNL +   + L   V  +E   
Sbjct: 31  VDNVVYKLYFKGLVSGETATDKKAIVKVGFGVAICDEADNLFYGMNKSLYEAVINREE-- 90

Query: 228 ECAELEALVEGLHIALVLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGK 287
             A++ AL+ GL+ ++  G+K V   C D  +YQ II    P   ++  L+ EV  L+G+
Sbjct: 91  --ADILALITGLYESIHRGVKNVVICCDDRQIYQTIIGREKPQQKNV-HLLEEVQRLRGR 150

Query: 288 FTYCNPSLVTRNDIKFAFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSV 347
                  LV   D  FA RLA +A+V       ++   K L  TC+IC +    + M   
Sbjct: 151 LASTGTVLVATRDDNFALRLAIDALV-------KATQEKPL--TCSICSDKTDAEHMLLN 210

Query: 348 DGCLHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRI 407
           D CLHR+CFSC+KQ ++++L   +G+   C   GC SE+ +ESC   L  ++IE+  +++
Sbjct: 211 DKCLHRHCFSCVKQQVKVKL--RSGIVPPCLEDGCKSELTLESCSMVLTPKLIEMWKRKM 270

Query: 408 KEASMPVLEKVYCPYSRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAP 467
           +E  +P  EK+YCPY  CS LMSK+EL +       +AE++  R C+KC+  FCI+CK P
Sbjct: 271 EEDLIPDAEKIYCPYRSCSMLMSKTELSR-------EAEQSNVRACIKCSELFCIDCKVP 330

Query: 468 WHYNMTCYDFRKLNPVLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEF 527
           WH +++C D+++++     ++ MLK LA+ ++WRQC  C  M+EL EGC HITCRCGYEF
Sbjct: 331 WHSDLSCADYKRIHSERLVNDMMLKVLANDQMWRQCSECKHMIELTEGCNHITCRCGYEF 389

Query: 528 CYTCGAPWKGKKPTCTCPIWDER 550
           CY CG  W  K    +C + D R
Sbjct: 391 CYRCGHKWT-KYHHYSCQLMDNR 389

BLAST of Carg21070 vs. Swiss-Prot
Match: sp|P0CE10|DEAHB_ARATH (ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01020 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-14
Identity = 53/204 (25.98%), Postives = 88/204 (43.14%), Query Frame = 0

Query: 331  CTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIESC 390
            C IC  +  VD  +S++GC H +C +C+ +  E  + N +     C H+ C + I +   
Sbjct: 1561 CPICLSE--VDDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVVADM 1620

Query: 391  GKFL-DSRVIEIISQRIKEASMPVLEKV-YCPYSRCSALMSKSELLKYSEASYFDAERTG 450
               L   ++ E+IS  +         K+ +C    C ++   +   +  E          
Sbjct: 1621 RALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSIYRVAGPQESGEPFI------- 1680

Query: 451  ARKCMKCNLFFCINCKAPWHYNMTCYDFRKL--NPVLRPDEEMLKSLASKKLWRQCVTCN 510
               C  C+   C  C   +H  +TC  ++K   NP L      LK  A  K  ++C  C 
Sbjct: 1681 ---CGACHSETCTRCHLEYHPLITCERYKKFKENPDL-----SLKDWAKGKDVKECPICK 1740

Query: 511  TMVELAEGCYHITCRCGYEFCYTC 531
            + +E  +GC H+ CRCG   C+TC
Sbjct: 1741 STIEKTDGCNHLQCRCGKHICWTC 1747

BLAST of Carg21070 vs. Swiss-Prot
Match: sp|F4KGU4|DEAHC_ARATH (ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10370 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.1e-13
Identity = 52/204 (25.49%), Postives = 86/204 (42.16%), Query Frame = 0

Query: 331  CTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIESC 390
            C IC  +  VD  +S++GC H +C +C+ +  E  + N +     C H+ C + I +   
Sbjct: 1564 CPICLSE--VDDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVLADM 1623

Query: 391  GKFL-DSRVIEIISQRIKEASMPVLEKV-YCPYSRCSALMSKSELLKYSEASYFDAERTG 450
               L   ++ E+ S  +         K  +C    C ++   +   +  E          
Sbjct: 1624 RALLSQEKLDELFSASLSSFVTSSDGKFRFCSTPDCPSVYRVAGPQESGEPFI------- 1683

Query: 451  ARKCMKCNLFFCINCKAPWHYNMTCYDFRKL--NPVLRPDEEMLKSLASKKLWRQCVTCN 510
               C  C+   C  C   +H  +TC  ++K   NP L      LK  A  K  ++C  C 
Sbjct: 1684 ---CGACHSEICTRCHLEYHPLITCERYKKFKENPDL-----SLKDWAKGKNVKECPICK 1743

Query: 511  TMVELAEGCYHITCRCGYEFCYTC 531
            + +E  +GC H+ CRCG   C+TC
Sbjct: 1744 STIEKTDGCNHMKCRCGKHICWTC 1750

BLAST of Carg21070 vs. Swiss-Prot
Match: sp|Q6T486|RBRA_DICDI (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.3e-13
Identity = 62/216 (28.70%), Postives = 91/216 (42.13%), Query Frame = 0

Query: 330 TCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLEIRLLNG-NGMQANCPHLGCTSEINIE 389
           +C IC ED    Q F++  C HRYC  C K  LEI++  G   +   CP   C   ++ +
Sbjct: 138 SCLICLEDYPPTQTFALI-CNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQD 197

Query: 390 SCGKFLDSRVIEIISQRIKEASMPVLEKV-YCPYSRCSALMSKSELLKYSEASYFDAERT 449
           +  + +   V E  +  I ++ +    +V +CP   C   +                ER 
Sbjct: 198 AFKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGCIYSIRCDR-----------KERK 257

Query: 450 GARKCMKCNLFFCINC---KAPWHYNMTCYDFRK-LNPVLRPDEEMLKSLASKKLWRQCV 509
            A  C KC   +C NC   +   H    C    K L       E +   LA+ K   +C 
Sbjct: 258 EAVNC-KCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCP 317

Query: 510 TCNTMVELAEGCYHITCR-----CGYEFCYTCGAPW 535
            C + +E   GC H+TCR     CG+EFC+ C  PW
Sbjct: 318 ECRSPIEKNGGCMHMTCRKNAGGCGFEFCWLCRGPW 337

BLAST of Carg21070 vs. Swiss-Prot
Match: sp|Q9US46|ITT1_SCHPO (E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=itt1 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.6e-12
Identity = 61/244 (25.00%), Postives = 98/244 (40.16%), Query Frame = 0

Query: 328 KETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEI-- 387
           K  C +CF++      F +  C H  C SC++    + +  G   Q  C  L C  +   
Sbjct: 171 KFQCNVCFDEFNGTDCFQLTRCGHVSCQSCLRDYYTMCIQEGMFSQIKCIDLDCGKDAPV 230

Query: 388 ----NIES-CGKFLDSRVIEIISQRIKEASMPVLEKVYCPYSRCSALMSKSELLKYSEAS 447
                +ES  G  L +R  E+  +R  E    +   ++CP S C     +    K +   
Sbjct: 231 LTLKELESIVGVQLTNRYKELEEKRRYENDSNI---IFCPRSFCQGPSKRDPGQKLA--- 290

Query: 448 YFDAERTGARKCMKCNLFFCINCKAPWHYNMT-CY---DFRKLNPVL------RPDE--- 507
                      C KC+  FC  C+A WH +++ C    D +KL  +        P++   
Sbjct: 291 ----------ICQKCDFAFCSFCQATWHGDLSPCKLEGDSKKLVEMYLNYQENEPEKALE 350

Query: 508 --------------EMLKSLASKKLW-----RQCVTCNTMVELAEGCYHITCRCGYEFCY 533
                         E +K+    + W     ++C TC+ +VE  +GC H+ C CG  FC+
Sbjct: 351 LEKRYGKRIIDRLVEQVKNDEEAEKWVLLNGQRCPTCDRVVERIDGCCHMNCLCGTHFCF 398

BLAST of Carg21070 vs. Swiss-Prot
Match: sp|Q4KLT0|RN217_XENLA (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.2e-11
Identity = 52/215 (24.19%), Postives = 86/215 (40.00%), Query Frame = 0

Query: 330 TCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDLEIRLLNGNGMQANCPHLGCTSEINIES 389
           +C +C ED  +  +     C    C  C+K+ L  ++  G   +  CP         I  
Sbjct: 2   SCRVCLEDRSIKPL---PCCKKPVCDECLKRYLSSQVQLGQA-EIQCP---------ITE 61

Query: 390 CGKFLDSRVI-------EIISQR-IKEASMPVLEKVYCPYSRCSALMSKSELLKYSEASY 449
           C K LD   I       +II  +   E S        CP  +      +   +     S 
Sbjct: 62  CNKHLDESTILYSLPHDDIIKYKYFLELSRMDSSTKPCPQCKHFTTFKRKTHIPNPTKS- 121

Query: 450 FDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPVLRPDEEMLKSLASKKLWRQ 509
              E     +C  C   +C  C APWH  + C +++K + +LR     ++    ++  ++
Sbjct: 122 ---ENKLKIQCPSCQFIWCFRCHAPWHEGVNCREYKKGDKLLRHWANEIEH--GQRNAQK 181

Query: 510 CVTCNTMVELAEGCYHITC-RCGYEFCYTCGAPWK 536
           C  C   ++  EGC H+TC +C   FCY CG  ++
Sbjct: 182 CPRCKVHIQRTEGCDHMTCSQCNTNFCYRCGERYR 197

BLAST of Carg21070 vs. TrEMBL
Match: tr|A0A1S3CJH8|A0A1S3CJH8_CUCME (RBR-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103501665 PE=3 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 2.2e-248
Identity = 424/553 (76.67%), Postives = 477/553 (86.26%), Query Frame = 0

Query: 4   MADTV-DDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATV 63
           MADT+ DDD +Q+LIT+QRSE+L+AKTL SDLD A+QLQ+QEAM+ASL S  S +  +++
Sbjct: 1   MADTLNDDDCVQELITQQRSELLSAKTLFSDLDYAFQLQLQEAMDASLTSIPSIESSSSL 60

Query: 64  NRVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFA 123
           N   PVL+SEET  LD ATTL+LEDIAR AME KDRE CQ EMRKMKEELDRRIHDQ FA
Sbjct: 61  NTADPVLDSEETSGLDLATTLMLEDIARFAMEFKDREHCQTEMRKMKEELDRRIHDQKFA 120

Query: 124 NYILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLIS 183
            YI +VP++EW +YGDNY       XXXXXXXXX           +DSECFRVYSKGLIS
Sbjct: 121 EYIRSVPENEWREYGDNYEKPYGESXXXXXXXXXWN---------VDSECFRVYSKGLIS 180

Query: 184 EERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIAL 243
           EERIRD  VRVAGIG+AVCDPKDNLLFEK  P+ES+V+GKETS ECAELEALVEGL++AL
Sbjct: 181 EERIRDTVVRVAGIGIAVCDPKDNLLFEKMSPIESMVEGKETSNECAELEALVEGLNVAL 240

Query: 244 VLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKF 303
           +LGLK +TFFCADYMLYQY+   V P +SS A LVNEV +LQGKFTYCNPSLVTRNDIKF
Sbjct: 241 ILGLKSITFFCADYMLYQYLTGRVPPATSSTAKLVNEVVVLQGKFTYCNPSLVTRNDIKF 300

Query: 304 AFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDL 363
           AF+LAREAIVSQITWPAE++NG CLKETCTICFEDV VDQMFSVDGCLHRYCFSCMKQ +
Sbjct: 301 AFKLAREAIVSQITWPAEADNGNCLKETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQHV 360

Query: 364 EIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYS 423
           E++LLNGNGMQANCPH GCTSE+NIESCGKFL+ +V EI+SQRIKEAS+P+ EKVYCPYS
Sbjct: 361 EVKLLNGNGMQANCPHQGCTSEVNIESCGKFLEPKVFEIMSQRIKEASVPIQEKVYCPYS 420

Query: 424 RCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPV 483
           RCSALMSK+ELL+Y+EASY DAERTGARKCMKCN FFCINCK  WHYN+TCYD+R LNP 
Sbjct: 421 RCSALMSKTELLRYTEASYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDYRNLNPN 480

Query: 484 LRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCT 543
           L PDE+MLKSLA++KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KKPTC 
Sbjct: 481 LHPDEQMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTCK 540

Query: 544 CPIWDERNIIRNQ 556
           CPIWDERNII  +
Sbjct: 541 CPIWDERNIIHRR 544

BLAST of Carg21070 vs. TrEMBL
Match: tr|A0A0A0LXM8|A0A0A0LXM8_CUCSA (RBR-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G088460 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 4.9e-248
Identity = 433/554 (78.16%), Postives = 482/554 (87.00%), Query Frame = 0

Query: 4   MADTV-DDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATV 63
           MADT+ DDD +QDLIT+QRSEIL+AKTL SDLD A+QLQ+QEAM+ASL SK S+   +++
Sbjct: 1   MADTMNDDDCVQDLITQQRSEILSAKTLFSDLDFAFQLQLQEAMDASLASKPSTDSSSSL 60

Query: 64  NRVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFA 123
           N   PVL+SE+T  LD ATTL+LEDIAR AME KDREQCQ EMRKMKEELDRRIHDQ FA
Sbjct: 61  NTPDPVLDSEDTSGLDLATTLMLEDIARFAMEFKDREQCQTEMRKMKEELDRRIHDQKFA 120

Query: 124 NYILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLIS 183
            +I +VP++EW +YGDNY      XXXXXXXXXXXXX        +DSEC RVYSKGLIS
Sbjct: 121 EFIRSVPENEWREYGDNYEKPYGEXXXXXXXXXXXXXS-------VDSECCRVYSKGLIS 180

Query: 184 EERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIAL 243
           EERIRDM VRVAGIGVAVCDPKDNLLFEK  P+ES+V+GKETS ECAELEALVEGL++AL
Sbjct: 181 EERIRDMVVRVAGIGVAVCDPKDNLLFEKMSPIESMVEGKETSSECAELEALVEGLNVAL 240

Query: 244 VLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKF 303
           +LGLK VTFFCADYMLYQY+   V P +SS   LVNEV +LQGKFTYCNPSLVTRNDIKF
Sbjct: 241 ILGLKTVTFFCADYMLYQYLTGRVPPATSSTEKLVNEVVVLQGKFTYCNPSLVTRNDIKF 300

Query: 304 AFRLAREAIVSQITWPAESENGKCLK-ETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQD 363
           AF+LAREAIVSQITWPA++ENGKCLK ETCTICFEDV VDQMFSVDGCLHRYCFSCMKQ 
Sbjct: 301 AFKLAREAIVSQITWPAQAENGKCLKEETCTICFEDVSVDQMFSVDGCLHRYCFSCMKQH 360

Query: 364 LEIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPY 423
           +E+RLLNGNGMQANCPH GCTSE+NIESCGKFL+ +V EI+SQRIKEAS+PV EKVYCPY
Sbjct: 361 VEVRLLNGNGMQANCPHQGCTSELNIESCGKFLEPKVFEIMSQRIKEASVPVQEKVYCPY 420

Query: 424 SRCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNP 483
           SRCSALMSK++LL+Y+EA Y DAERTGARKCMKCN FFCINCK  WHYN+TCYD RKLNP
Sbjct: 421 SRCSALMSKTDLLRYTEAFYIDAERTGARKCMKCNQFFCINCKVAWHYNLTCYDHRKLNP 480

Query: 484 VLRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTC 543
            L PDE+MLKSLA++KLWRQC+ CN MVELAEGCYHITCRCGYEFCYTCGAPWK KKPTC
Sbjct: 481 NLHPDEKMLKSLATRKLWRQCIVCNNMVELAEGCYHITCRCGYEFCYTCGAPWKNKKPTC 540

Query: 544 TCPIWDERNIIRNQ 556
            CPIWDERNII  +
Sbjct: 541 KCPIWDERNIIHRR 547

BLAST of Carg21070 vs. TrEMBL
Match: tr|A0A251NMJ3|A0A251NMJ3_PRUPE (RBR-type E3 ubiquitin transferase OS=Prunus persica OX=3760 GN=PRUPE_6G094500 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.4e-186
Identity = 329/553 (59.49%), Postives = 410/553 (74.14%), Query Frame = 0

Query: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVN 63
           M D  D D L  +++EQR +++AAKTL SDLD+A++LQMQEAM+ASL             
Sbjct: 1   MDDRTDPDDLHTILSEQRRDLMAAKTLDSDLDMAFKLQMQEAMSASLALNPXXXXXXXXX 60

Query: 64  RVVPVLESEETGA-LDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFA 123
                       A LD A TL+LED+ R A E +D E+  +EM K KE+L+RRIHDQ FA
Sbjct: 61  XXXXXXXXXXXDAILDLAATLMLEDVERFAQEWEDHERTVSEMMKTKEDLNRRIHDQKFA 120

Query: 124 NYILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLIS 183
             + +VPDD W+K+GD Y                 XXXXXXX   +++E  R+Y KGL+S
Sbjct: 121 ADLCDVPDDYWAKHGDYY--------ERPYSADESXXXXXXXXAAVETENLRLYCKGLVS 180

Query: 184 EERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIAL 243
           EER+RDM V VAG GVA+CDP+DNL+FE  + LE+V DG   S E AELEA++EGL+ AL
Sbjct: 181 EERVRDMKVVVAGAGVAICDPRDNLIFEARKNLEAVADGVVLSNEAAELEAIIEGLNKAL 240

Query: 244 VLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKF 303
            L LK VTF+C DYMLYQY+   V PG+S +ATLVN+V+LLQ KF YC+PSLVTR DIKF
Sbjct: 241 TLDLKSVTFYCDDYMLYQYVTNRVRPGNSKVATLVNQVALLQRKFEYCSPSLVTRTDIKF 300

Query: 304 AFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDL 363
           A ++AREAIVSQITW A+S NGK LKETC ICFE+  V +MFS+DGCLHRYC SCMKQ +
Sbjct: 301 ALKVAREAIVSQITWRADSSNGKSLKETCVICFEETDVAEMFSIDGCLHRYCCSCMKQHV 360

Query: 364 EIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYS 423
           E++ LNG G  A CPH GC +E+NI+SC KFL  +++E ISQRIKE+S+PV +KVYCP  
Sbjct: 361 EVKFLNGMG--AECPHEGCKNEVNIDSCAKFLAPKLVEAISQRIKESSIPVTDKVYCPNP 420

Query: 424 RCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPV 483
           RCSALMSK E+L+Y++ ++  AE+TGAR+CMKC+ +FCINCK PWH+NMTCYD+++ +P 
Sbjct: 421 RCSALMSKKEVLEYTKTTFVRAEQTGARRCMKCHYYFCINCKVPWHFNMTCYDYKRSHPY 480

Query: 484 LRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCT 543
              ++++L SLA+KKLWRQCV CN MVELAEGCYHITCRCGYEFCYTCGA WK KK TC+
Sbjct: 481 PHREDQLLNSLATKKLWRQCVKCNHMVELAEGCYHITCRCGYEFCYTCGAEWKNKKATCS 540

Query: 544 CPIWDERNIIRNQ 556
           C IWDERNIIR Q
Sbjct: 541 CRIWDERNIIREQ 543

BLAST of Carg21070 vs. TrEMBL
Match: tr|A0A251NMM0|A0A251NMM0_PRUPE (RBR-type E3 ubiquitin transferase OS=Prunus persica OX=3760 GN=PRUPE_6G094500 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 2.4e-186
Identity = 329/553 (59.49%), Postives = 410/553 (74.14%), Query Frame = 0

Query: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLGSKSSSKRPATVN 63
           M D  D D L  +++EQR +++AAKTL SDLD+A++LQMQEAM+ASL             
Sbjct: 1   MDDRTDPDDLHTILSEQRRDLMAAKTLDSDLDMAFKLQMQEAMSASLALNPXXXXXXXXX 60

Query: 64  RVVPVLESEETGA-LDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFA 123
                       A LD A TL+LED+ R A E +D E+  +EM K KE+L+RRIHDQ FA
Sbjct: 61  XXXXXXXXXXXDAILDLAATLMLEDVERFAQEWEDHERTVSEMMKTKEDLNRRIHDQKFA 120

Query: 124 NYILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLIS 183
             + +VPDD W+K+GD Y                 XXXXXXX   +++E  R+Y KGL+S
Sbjct: 121 ADLCDVPDDYWAKHGDYY--------ERPYSADESXXXXXXXXAAVETENLRLYCKGLVS 180

Query: 184 EERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIAL 243
           EER+RDM V VAG GVA+CDP+DNL+FE  + LE+V DG   S E AELEA++EGL+ AL
Sbjct: 181 EERVRDMKVVVAGAGVAICDPRDNLIFEARKNLEAVADGVVLSNEAAELEAIIEGLNKAL 240

Query: 244 VLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKF 303
            L LK VTF+C DYMLYQY+   V PG+S +ATLVN+V+LLQ KF YC+PSLVTR DIKF
Sbjct: 241 TLDLKSVTFYCDDYMLYQYVTNRVRPGNSKVATLVNQVALLQRKFEYCSPSLVTRTDIKF 300

Query: 304 AFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDL 363
           A ++AREAIVSQITW A+S NGK LKETC ICFE+  V +MFS+DGCLHRYC SCMKQ +
Sbjct: 301 ALKVAREAIVSQITWRADSSNGKSLKETCVICFEETDVAEMFSIDGCLHRYCCSCMKQHV 360

Query: 364 EIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYS 423
           E++ LNG G  A CPH GC +E+NI+SC KFL  +++E ISQRIKE+S+PV +KVYCP  
Sbjct: 361 EVKFLNGMG--AECPHEGCKNEVNIDSCAKFLAPKLVEAISQRIKESSIPVTDKVYCPNP 420

Query: 424 RCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPV 483
           RCSALMSK E+L+Y++ ++  AE+TGAR+CMKC+ +FCINCK PWH+NMTCYD+++ +P 
Sbjct: 421 RCSALMSKKEVLEYTKTTFVRAEQTGARRCMKCHYYFCINCKVPWHFNMTCYDYKRSHPY 480

Query: 484 LRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCT 543
              ++++L SLA+KKLWRQCV CN MVELAEGCYHITCRCGYEFCYTCGA WK KK TC+
Sbjct: 481 PHREDQLLNSLATKKLWRQCVKCNHMVELAEGCYHITCRCGYEFCYTCGAEWKNKKATCS 540

Query: 544 CPIWDERNIIRNQ 556
           C IWDERNIIR Q
Sbjct: 541 CRIWDERNIIREQ 543

BLAST of Carg21070 vs. TrEMBL
Match: tr|A0A2I4EHJ4|A0A2I4EHJ4_9ROSI (RBR-type E3 ubiquitin transferase OS=Juglans regia OX=51240 GN=LOC108989645 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.2e-183
Identity = 324/557 (58.17%), Postives = 416/557 (74.69%), Query Frame = 0

Query: 4   MADTVDDDYLQDLITEQRSEILAAKTLISDLDLAYQLQMQEAMNASLG-SKSSSKRPATV 63
           MA +   D L   ++EQ   +  AKTL SDLDLA++LQMQEAM+AS+    SSS+  A  
Sbjct: 1   MAKSSVMDDLNAALSEQSRALKVAKTLDSDLDLAFKLQMQEAMSASIALGPSSSRGKAQK 60

Query: 64  NRVVPVLESEETGALDFATTLLLEDIARIAMEHKDREQCQAEMRKMKEELDRRIHDQNFA 123
            +   V+ S    + D A TL+L+++ R+  E +DR+Q    MRKM+E+LD RIHDQ  A
Sbjct: 61  PQPNDVVSSPYDDSRDVAATLMLQEVDRVMRELEDRKQSXXXMRKMREDLDLRIHDQKLA 120

Query: 124 NYILNVPDDEWSKYGDNYXXXXXXXXXXXXXXXXXXXXXXXXRGLLDSECFRVYSKGLIS 183
             ILN+ D+EW   GDNY               XXXXXXXXX  L+D+ECFR+Y KGL+S
Sbjct: 121 AEILNISDEEWKINGDNY-------HRPYRMKAXXXXXXXXXXXLVDTECFRLYFKGLVS 180

Query: 184 EERIRDMTVRVAGIGVAVCDPKDNLLFEKTRPLESVVDGKETSCECAELEALVEGLHIAL 243
           EE +RDM V VAG GVA+CDP+D+L+ E  + +E++VDG+  + E AELEAL+EGL  AL
Sbjct: 181 EESVRDMKVTVAGAGVAICDPRDSLILEARKNVEALVDGQVVTNEVAELEALIEGLENAL 240

Query: 244 VLGLKKVTFFCADYMLYQYIIRSVAPGSSSMATLVNEVSLLQGKFTYCNPSLVTRNDIKF 303
            L LK++TFFC DYMLYQY+   V P  S + TLVN+V+LLQ KF +CNPS V RNDIKF
Sbjct: 241 SLDLKRLTFFCDDYMLYQYVTGRVLPRQSHVVTLVNQVTLLQRKFVFCNPSFVARNDIKF 300

Query: 304 AFRLAREAIVSQITWPAESENGKCLKETCTICFEDVCVDQMFSVDGCLHRYCFSCMKQDL 363
           AF+ AR+A+VSQITWP E+  GK LKETC ICFED   ++MFSVDGCLHRYCFSCMKQ +
Sbjct: 301 AFKSARDAVVSQITWPEETSKGKSLKETCVICFEDTDANKMFSVDGCLHRYCFSCMKQHV 360

Query: 364 EIRLLNGNGMQANCPHLGCTSEINIESCGKFLDSRVIEIISQRIKEASMPVLEKVYCPYS 423
           E++LLNG G  A CPH GC SE+N++SCGKFL  +++E+++QR+KE+S+PV+++VYCPY 
Sbjct: 361 EVKLLNGMG--AKCPHEGCNSEVNVDSCGKFLAPQLVEVMTQRMKESSIPVIQRVYCPYP 420

Query: 424 RCSALMSKSELLKYSEASYFDAERTGARKCMKCNLFFCINCKAPWHYNMTCYDFRKLNPV 483
           RCSALMSKSE+L+Y++     AE++G RKCMKC+ FFCINCK PWH+ M+CYD+++ NP 
Sbjct: 421 RCSALMSKSEVLQYTKNINVTAEQSGLRKCMKCHYFFCINCKVPWHFKMSCYDYKRSNPH 480

Query: 484 LRPDEEMLKSLASKKLWRQCVTCNTMVELAEGCYHITCRCGYEFCYTCGAPWKGKKPTCT 543
             P++  LKSLA+ K WRQC  CN ++ELAEGCYHITCRCGYEFCYTCGA WK KK TC+
Sbjct: 481 SHPEDAKLKSLATTKHWRQCQKCNHVIELAEGCYHITCRCGYEFCYTCGAQWKNKKATCS 540

Query: 544 CPIWDERNIIRNQRQRR 560
           CPIWD RNIIR++++RR
Sbjct: 541 CPIWDVRNIIRDEQRRR 548

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022964983.10.0e+0099.82uncharacterized protein LOC111464930 [Cucurbita moschata][more]
XP_023519052.11.2e-30998.38uncharacterized protein LOC111782521 [Cucurbita pepo subsp. pepo][more]
XP_022970417.13.7e-30096.05uncharacterized protein LOC111469406 [Cucurbita maxima][more]
XP_022157931.11.1e-25179.82uncharacterized protein LOC111024540 [Momordica charantia][more]
XP_008463535.13.3e-24876.67PREDICTED: uncharacterized protein LOC103501665 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G60250.12.2e-10044.87zinc finger (C3HC4-type RING finger) family protein[more]
AT2G25370.14.7e-7942.16RING/U-box superfamily protein[more]
AT2G19610.26.8e-7840.37RING/U-box superfamily protein[more]
AT3G45580.19.8e-7740.48RING/U-box protein with C6HC-type zinc finger[more]
AT5G37560.14.1e-7538.90RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
sp|P0CE10|DEAHB_ARATH3.0e-1425.98ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
sp|F4KGU4|DEAHC_ARATH1.1e-1325.49ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
sp|Q6T486|RBRA_DICDI3.3e-1328.70Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G... [more]
sp|Q9US46|ITT1_SCHPO3.6e-1225.00E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
sp|Q4KLT0|RN217_XENLA5.2e-1124.19Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CJH8|A0A1S3CJH8_CUCME2.2e-24876.67RBR-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103501665 PE=3 S... [more]
tr|A0A0A0LXM8|A0A0A0LXM8_CUCSA4.9e-24878.16RBR-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G088460 PE=... [more]
tr|A0A251NMJ3|A0A251NMJ3_PRUPE2.4e-18659.49RBR-type E3 ubiquitin transferase OS=Prunus persica OX=3760 GN=PRUPE_6G094500 PE... [more]
tr|A0A251NMM0|A0A251NMM0_PRUPE2.4e-18659.49RBR-type E3 ubiquitin transferase OS=Prunus persica OX=3760 GN=PRUPE_6G094500 PE... [more]
tr|A0A2I4EHJ4|A0A2I4EHJ4_9ROSI3.2e-18358.17RBR-type E3 ubiquitin transferase OS=Juglans regia OX=51240 GN=LOC108989645 PE=3... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
GO:0003676nucleic acid binding
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR017907Znf_RING_CS
IPR031127E3_UB_ligase_RBR
IPR036397RNaseH_sf
IPR013083Znf_RING/FYVE/PHD
IPR002867IBR_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004842 ubiquitin-protein transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21070-RACarg21070-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 91..118
NoneNo IPR availablePFAMPF13456RVT_3coord: 193..310
e-value: 7.8E-16
score: 57.9
NoneNo IPR availableGENE3DG3DSA:1.20.120.1750coord: 471..560
e-value: 2.6E-9
score: 39.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 144..163
NoneNo IPR availablePANTHERPTHR11685:SF132SUBFAMILY NOT NAMEDcoord: 158..543
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 330..412
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 487..550
IPR002867IBR domainSMARTSM00647ibrneu5coord: 483..543
e-value: 0.0099
score: 24.1
coord: 400..473
e-value: 2.6E-14
score: 63.6
IPR002867IBR domainPFAMPF01485IBRcoord: 491..541
e-value: 5.0E-8
score: 33.0
coord: 405..473
e-value: 2.4E-12
score: 46.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 325..409
e-value: 5.9E-13
score: 50.7
IPR036397Ribonuclease H superfamilyGENE3DG3DSA:3.30.420.10coord: 175..312
e-value: 4.8E-7
score: 31.8
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 158..543
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 349..358
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 331..377
score: 9.022