Carg20991 (gene) Silver-seed gourd

Overview
NameCarg20991
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionE3 ubiquitin-protein ligase at3g02290-like protein
LocationCucurbita_argyrosperma_scaffold_170 : 219509 .. 221151 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTGCAGTGCCCGCTGTCTATGGAGGAGCGTGAGTCATCCGAAATTCATGGTGGCTCAGCGTCCATGCTCCTCGGTGCAGGACATGATTTGAACCTGGATTTATCGATACCGAACACTTACCGAGCCCCTCCGACACCTCTCCCATATGATGCAGCTTTGAAATGTCCACAACCGAAAGATCTCAACTCTTTTAAAGAAACAAAATGTGGATGCAGTTTTAAAACTTCATCAGCATTGAAAATTGTTGGGGAACTGGATTGTAAGTTCCAAGACAGCCCTTTGCTCGGAGGCCCTCCACGGAAACTCGATCATTCCAAGTCGAAAGGCAATACTCCTTGTCCGGTCACCGAAGAAGAGGATGTCTGTCCCATTTGTCTTGAAGGTGAGTTGCATTGGCATCTATAGAAACTGATACTCTTCTGCATATCATTCTTCTTTTCACTTATCAAATCACCAATTCATGTTTGTTTAACATTGTGTCTCCTATGTAGAGTATGGTTCTGAACATCCAGAAATTATCACAAAATGCAAGCATCACTTTCACCTTGCTTGCCTTCTCGAGTGGACCGAAAGAAGCAATGCCTGTCCTATCTGCGACAAGGTTTGTTCTAATGAGTTACGAGTATGCTGACAGAATTCGAATCTCGATCTTGTTAGCTATAATAGCCCAAGCCTACCGCTAACAGATATTGTCCTCTTTAGGCTTTCCCTTTCGGGTTTTCCCTTTCGGGTTTTTCCTCAAGGTTTTTAAAATGAGTCTGCCAAGAAGGATTTTCAGCACTTATAAAGAATGCTTCATTCCCCATTCCAACTGACGTGGGATCTCACAATCTACCTCCCTTTCTAGCCCAACGTCCTCGCTGACACACCGGCCCGTGACTGGTTCTGGTACAATTTGTAACAGCCCAAACCCACCACTAACAGATATTGTCCGTTTTGACTCGTTATATATCGTCATCAGCCTCACGGTTTTAAAAACGCATTTTATAACCTTGAGGGAAAGTCTAGAAGCCAAAGAGGACAATATTTGTTAGCAGTGGGCTTGGGACGTAACAAGCACTGTCCATCATTTACGTTGCCATATATGACAATTTTTCGAGTTCTATATAAATAAAAAAACACTTCCAAAGCTGATCGAACTTTTTGCTCGATTTTTTTATGCAGGAGACGATATTTGAGCTACCTTGAGTCTGCAGAAATGAGAAACTTCTCTAGTCCTTAAAATTCAAGCTTGAGCTCAGTTCATCACTGCGTTCAATTACAGGTTCATTTTCAAAACCATGGCTGTATAGATAAGTTGATTGCTTCATGGCTGCAAGTTCGAGATATAAATTGTCGTAGGCAGTCGATTTGATACATCGGCCGAGGAAGAAATAATGGTCTGGTTCCTCATGTTGTTGGATGTTCATGCATATTCATTCTTTTTTTCAATATTTGATCATCAGTTCTGATGGAACTCTCGTAAGATTCATACATGTCTTTCTCATTTATCTCTAGCTTTATGATCTCTCCAACTTTGTGTAGTTTTTAGATAGTTTAAATATATCATACATTTCTATATTGAAATCTAACACTGTATATGAATAAGAATTTTAGTACTGATAGCTGTTGTCGGTGAATACAAATTATTTTTGTAACA

mRNA sequence

CTTTCTGCAGTGCCCGCTGTCTATGGAGGAGCGTGAGTCATCCGAAATTCATGGTGGCTCAGCGTCCATGCTCCTCGGTGCAGGACATGATTTGAACCTGGATTTATCGATACCGAACACTTACCGAGCCCCTCCGACACCTCTCCCATATGATGCAGCTTTGAAATGTCCACAACCGAAAGATCTCAACTCTTTTAAAGAAACAAAATGTGGATGCAGTTTTAAAACTTCATCAGCATTGAAAATTGTTGGGGAACTGGATTGTAAGTTCCAAGACAGCCCTTTGCTCGGAGGCCCTCCACGGAAACTCGATCATTCCAAGTCGAAAGGCAATACTCCTTGTCCGGTCACCGAAGAAGAGGATGTCTGTCCCATTTGTCTTGAAGAGTATGGTTCTGAACATCCAGAAATTATCACAAAATGCAAGCATCACTTTCACCTTGCTTGCCTTCTCGAGTGGACCGAAAGAAGCAATGCCTGTCCTATCTGCGACAAGGAGACGATATTTGAGCTACCTTGAGTCTGCAGAAATGAGAAACTTCTCTAGTCCTTAAAATTCAAGCTTGAGCTCAGTTCATCACTGCGTTCAATTACAGGTTCATTTTCAAAACCATGGCTGTATAGATAAGTTGATTGCTTCATGGCTGCAAGTTCGAGATATAAATTGTCGTAGGCAGTCGATTTGATACATCGGCCGAGGAAGAAATAATGGTCTGGTTCCTCATGTTGTTGGATGTTCATGCATATTCATTCTTTTTTTCAATATTTGATCATCAGTTCTGATGGAACTCTCGTAAGATTCATACATGTCTTTCTCATTTATCTCTAGCTTTATGATCTCTCCAACTTTGTGTAGTTTTTAGATAGTTTAAATATATCATACATTTCTATATTGAAATCTAACACTGTATATGAATAAGAATTTTAGTACTGATAGCTGTTGTCGGTGAATACAAATTATTTTTGTAACA

Coding sequence (CDS)

ATGGAGGAGCGTGAGTCATCCGAAATTCATGGTGGCTCAGCGTCCATGCTCCTCGGTGCAGGACATGATTTGAACCTGGATTTATCGATACCGAACACTTACCGAGCCCCTCCGACACCTCTCCCATATGATGCAGCTTTGAAATGTCCACAACCGAAAGATCTCAACTCTTTTAAAGAAACAAAATGTGGATGCAGTTTTAAAACTTCATCAGCATTGAAAATTGTTGGGGAACTGGATTGTAAGTTCCAAGACAGCCCTTTGCTCGGAGGCCCTCCACGGAAACTCGATCATTCCAAGTCGAAAGGCAATACTCCTTGTCCGGTCACCGAAGAAGAGGATGTCTGTCCCATTTGTCTTGAAGAGTATGGTTCTGAACATCCAGAAATTATCACAAAATGCAAGCATCACTTTCACCTTGCTTGCCTTCTCGAGTGGACCGAAAGAAGCAATGCCTGTCCTATCTGCGACAAGGAGACGATATTTGAGCTACCTTGA

Protein sequence

MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKETKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPCPVTEEEDVCPICLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP
Homology
[Close]
BLAST of Carg20991 vs. NCBI nr
Match: XP_022980128.1 (probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima] >XP_022980129.1 probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima] >XP_022980130.1 probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima])

HSP 1 Score: 354.0 bits (907), Expect = 2.8e-94
Identity = 164/165 (99.39%), Postives = 164/165 (99.39%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE
Sbjct: 26  MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 85

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPCPVTEEEDVCPICL 120
           TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTP PVTEEEDVCPICL
Sbjct: 86  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPTPVTEEEDVCPICL 145

Query: 121 EEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP 166
           EEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP
Sbjct: 146 EEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP 190

[Close]
BLAST of Carg20991 vs. NCBI nr
Match: XP_022924496.1 (probable E3 ubiquitin-protein ligase RHB1A [Cucurbita moschata] >XP_022924497.1 probable E3 ubiquitin-protein ligase RHB1A [Cucurbita moschata] >XP_022924498.1 probable E3 ubiquitin-protein ligase RHB1A [Cucurbita moschata])

HSP 1 Score: 351.7 bits (901), Expect = 1.4e-93
Identity = 162/165 (98.18%), Postives = 163/165 (98.79%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEERESSEIHGGS SML+GAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE
Sbjct: 24  MEERESSEIHGGSGSMLIGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 83

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPCPVTEEEDVCPICL 120
           TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTP PVTEEEDVCPICL
Sbjct: 84  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPSPVTEEEDVCPICL 143

Query: 121 EEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP 166
           EEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP
Sbjct: 144 EEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP 188

[Close]
BLAST of Carg20991 vs. NCBI nr
Match: XP_023527676.1 (probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo] >XP_023527677.1 probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo] >XP_023527678.1 probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343.6 bits (880), Expect = 3.8e-91
Identity = 159/165 (96.36%), Postives = 162/165 (98.18%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEERESSEIHGGSAS+LLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQ K+LNSFKE
Sbjct: 24  MEERESSEIHGGSASILLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQLKELNSFKE 83

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPCPVTEEEDVCPICL 120
           TKCGCSFKTSSALK VGELDCKFQDSPLLGGPPRKLDHSKSKGNTP PVTEEEDVCPICL
Sbjct: 84  TKCGCSFKTSSALKTVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPTPVTEEEDVCPICL 143

Query: 121 EEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFELP 166
           EEYGSEHPEIIT+CKHHFHLACLLEWTERSNACPICDKETIFELP
Sbjct: 144 EEYGSEHPEIITQCKHHFHLACLLEWTERSNACPICDKETIFELP 188

[Close]
BLAST of Carg20991 vs. NCBI nr
Match: XP_008438020.1 (PREDICTED: E3 ubiquitin-protein ligase At3g02290 isoform X2 [Cucumis melo])

HSP 1 Score: 257.7 bits (657), Expect = 2.7e-65
Identity = 128/166 (77.11%), Postives = 138/166 (83.13%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEER +SEI+ GS+SMLL AGHDLNLD SIPNTYR+PPTPLPYD  LK PQ KD NS KE
Sbjct: 26  MEERMTSEINSGSSSMLLNAGHDLNLDFSIPNTYRSPPTPLPYDVVLKYPQQKDPNSAKE 85

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKG-NTPCPVTEE-EDVCPI 120
             CGCS KT+SA+K VGELD K QDS    GPPRKL+HSKSKG +T  PVTEE +DVCPI
Sbjct: 86  RICGCSLKTTSAVKSVGELDRKSQDS----GPPRKLEHSKSKGTSTTTPVTEEDQDVCPI 145

Query: 121 CLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFEL 165
           CLEEY S HPEIITKCKHHFHLACLLEWTERS+ CPICDKE IFEL
Sbjct: 146 CLEEYDSVHPEIITKCKHHFHLACLLEWTERSDVCPICDKEMIFEL 187

[Close]
BLAST of Carg20991 vs. NCBI nr
Match: XP_022147277.1 (probable E3 ubiquitin-protein ligase RHB1A [Momordica charantia])

HSP 1 Score: 254.6 bits (649), Expect = 2.3e-64
Identity = 125/165 (75.76%), Postives = 128/165 (77.58%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEERESSEI  GSASMLL AGHD +LDLSIPNTYRAPPTPLPYD   +CPQ KD NS KE
Sbjct: 26  MEERESSEIRSGSASMLLNAGHDSDLDLSIPNTYRAPPTPLPYDMVFRCPQSKDFNSIKE 85

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKG-NTPCPVTEEEDVCPIC 120
           T CGCSFKTS A KIVGE D K QDSPLLGGPPRKL+HSK KG NTP  V EEED CPIC
Sbjct: 86  TICGCSFKTSPAAKIVGEPDNKSQDSPLLGGPPRKLEHSKLKGNNTPTLVAEEEDACPIC 145

Query: 121 LEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFEL 165
           LEEY SEHPE ITKCKHHFHLACLLEWT             IFEL
Sbjct: 146 LEEYDSEHPESITKCKHHFHLACLLEWTXXXXXXXXXXXXMIFEL 190

[Close]
BLAST of Carg20991 vs. TAIR10
Match: AT4G00335.1 (RING-H2 finger B1A)

HSP 1 Score: 142.5 bits (358), Expect = 2.3e-34
Identity = 74/153 (48.37%), Postives = 95/153 (62.09%), Query Frame = 0 

Query: 11  GGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKETKCGCSFKTS 70
           G  ++   G   D+ L+ SIP+T+ A P PLPYD  L  PQ  D  S K    G SF+T 
Sbjct: 37  GVGSAFTTGLLVDIGLETSIPDTFCA-PAPLPYDLLLGRPQCTDSESIKGRMSGSSFETL 96

Query: 71  SALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPCPVTEEEDVCPICLEEYGSEHPEI 130
           +  + +GE DCK   S ++   PRK D SK +G     V EEED CPIC E+Y  E+P +
Sbjct: 97  ATCEDLGESDCKTLASSVILS-PRKSDFSKHQG-LKILVDEEEDCCPICFEDYDVENPRL 156

Query: 131 ITKCKHHFHLACLLEWTERSNACPICDKETIFE 164
            TKC+H FHL+CLLEW ERS+ CPICDKE +F+
Sbjct: 157 TTKCEHEFHLSCLLEWIERSDRCPICDKEVVFD 186

[Close]
BLAST of Carg20991 vs. TAIR10
Match: AT5G41350.1 (RING/U-box superfamily protein)

HSP 1 Score: 108.6 bits (270), Expect = 3.7e-24
Identity = 65/161 (40.37%), Postives = 83/161 (51.55%), Query Frame = 0 

Query: 23  DLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKETKC-------GCSFKTSSALKI 82
           D NL+ S P+ Y  PP P P+D  +  PQ     S +E  C         S  T SA + 
Sbjct: 53  DTNLETSSPDAYIPPPLPTPFDVPIGIPQTP--ASGEEATCVDIREVSVDSANTESAQET 112

Query: 83  VG-------------ELDCKFQDSPLLGGPPRKLDHSKSKGNTPCPVTEEEDVCPICLEE 142
           V              E D K Q    L     ++D   SK     P+ EEED CPICLEE
Sbjct: 113 VDGITLGVPTTCSHKETDSKIQTEIDLES-TEEIDPKLSKA-VFIPIEEEED-CPICLEE 172

Query: 143 YGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFE 164
           Y  E+P+++ KC HHFHLAC+LEW ERS  CP+C+KE +F+
Sbjct: 173 YDIENPKLVAKCDHHFHLACILEWMERSETCPVCNKEMVFD 208

[Close]
BLAST of Carg20991 vs. TAIR10
Match: AT5G38895.3 (RING/U-box superfamily protein)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-20
Identity = 54/148 (36.49%), Postives = 78/148 (52.70%), Query Frame = 0 

Query: 26  LDLSIPNTYRAPPTPLPYDAALKCPQ-PKDLNSFKETKCGCSFKTSSALKIVGELDCKFQ 85
           LD S+ + Y +PPTPLPYDA  +  +  K  +   E        T  + + +G+   K+ 
Sbjct: 174 LDDSLSSVYHSPPTPLPYDADPRYFRFVKGSSHSGEESEPLRGDTEMSSEALGDGGAKWS 233

Query: 86  DSPLLGGPPR-----KLDHSKSKGNTPCPV----TEEEDVCPICLEEYGSEHPEIITKCK 145
            S    G            +KSK      V    +++ED+CP CL++Y  E+P+IITKC 
Sbjct: 234 KSDSEDGSKEVYTKGSSTFTKSKTMPGIEVYYADSDDEDICPTCLDDYTLENPKIITKCS 293

Query: 146 HHFHLACLLEWTERSNACPICDKETIFE 164
           HHFHL+C+ EW ERS  CP+C K   F+
Sbjct: 294 HHFHLSCIYEWMERSETCPVCGKVMAFD 321

[Close]
BLAST of Carg20991 vs. TAIR10
Match: AT5G15790.1 (RING/U-box superfamily protein)

HSP 1 Score: 95.5 bits (236), Expect = 3.2e-20
Identity = 54/154 (35.06%), Postives = 82/154 (53.25%), Query Frame = 0 

Query: 27  DLSIPNTYRAPPTPLPYDAALKCPQPK--DLNSFKETKCGCSFKTSSALKIVGELD---- 86
           D  + NT+ + P PLPYDA  +  + +   L S ++     S + +  L+   ++D    
Sbjct: 71  DNFMSNTFHSTPRPLPYDADPRYFRSRRDSLVSRRDKGSSHSHEEAEPLRSDADVDSESF 130

Query: 87  ----CKFQDSPLLGGPPRKLDHSKSK-----------GNTPCPVT-EEEDVCPICLEEYG 146
                K+ +  ++ G   K + S+S             N    +T ++EDVCP CLEEY 
Sbjct: 131 SVEGSKWANKLIISGEDSKEEFSRSSRRILQSRTMSTSNEGLYITSDDEDVCPTCLEEYI 190

Query: 147 SEHPEIITKCKHHFHLACLLEWTERSNACPICDK 159
           SE+P+I+TKC HHFHL+C+ EW ERS  CP+C K
Sbjct: 191 SENPKIVTKCSHHFHLSCIYEWMERSENCPVCGK 224

[Close]
BLAST of Carg20991 vs. TAIR10
Match: AT3G02290.1 (RING/U-box superfamily protein)

HSP 1 Score: 89.0 bits (219), Expect = 3.0e-18
Identity = 33/50 (66.00%), Postives = 42/50 (84.00%), Query Frame = 0 

Query: 109 VTEEEDVCPICLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDK 159
           ++E+EDVCP CLEEY SE+P+I+TKC HHFHL+C+ EW ERS  CP+C K
Sbjct: 174 LSEDEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGK 223

[Close]
BLAST of Carg20991 vs. Swiss-Prot
Match: sp|Q2HIJ8|RHB1A_ARATH (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.2e-33
Identity = 74/153 (48.37%), Postives = 95/153 (62.09%), Query Frame = 0 

Query: 11  GGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKETKCGCSFKTS 70
           G  ++   G   D+ L+ SIP+T+ A P PLPYD  L  PQ  D  S K    G SF+T 
Sbjct: 37  GVGSAFTTGLLVDIGLETSIPDTFCA-PAPLPYDLLLGRPQCTDSESIKGRMSGSSFETL 96

Query: 71  SALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNTPCPVTEEEDVCPICLEEYGSEHPEI 130
           +  + +GE DCK   S ++   PRK D SK +G     V EEED CPIC E+Y  E+P +
Sbjct: 97  ATCEDLGESDCKTLASSVILS-PRKSDFSKHQG-LKILVDEEEDCCPICFEDYDVENPRL 156

Query: 131 ITKCKHHFHLACLLEWTERSNACPICDKETIFE 164
            TKC+H FHL+CLLEW ERS+ CPICDKE +F+
Sbjct: 157 TTKCEHEFHLSCLLEWIERSDRCPICDKEVVFD 186

[Close]
BLAST of Carg20991 vs. Swiss-Prot
Match: sp|Q8LE94|RING3_ARATH (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 5.5e-17
Identity = 33/50 (66.00%), Postives = 42/50 (84.00%), Query Frame = 0 

Query: 109 VTEEEDVCPICLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDK 159
           ++E+EDVCP CLEEY SE+P+I+TKC HHFHL+C+ EW ERS  CP+C K
Sbjct: 174 LSEDEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGK 223

[Close]
BLAST of Carg20991 vs. Swiss-Prot
Match: sp|Q93ZF6|AIRP1_ARATH (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.0e-15
Identity = 32/51 (62.75%), Postives = 40/51 (78.43%), Query Frame = 0 

Query: 111 EEEDVCPICLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETI 162
           E  D CPICLEEY  ++P+++TKC H FHLAC+L W ERS ACP+CDKE +
Sbjct: 99  EAIDECPICLEEYEIDNPKLLTKCGHDFHLACILAWMERSEACPVCDKELV 149

[Close]
BLAST of Carg20991 vs. Swiss-Prot
Match: sp|Q9ZT42|RHF2A_ARATH (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 22/44 (50.00%), Postives = 30/44 (68.18%), Query Frame = 0 

Query: 113 EDVCPICLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPIC 157
           +D C ICLE +    P  +T CKH +HL C+LEW +RS+ CP+C
Sbjct: 30  DDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMC 73

[Close]
BLAST of Carg20991 vs. Swiss-Prot
Match: sp|Q4TU14|RHF1A_ARATH (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-09
Identity = 21/41 (51.22%), Postives = 29/41 (70.73%), Query Frame = 0 

Query: 116 CPICLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPIC 157
           C ICLE +  + P  +T CKH +HL C++EW++RS  CPIC
Sbjct: 46  CSICLEPFTLQDPSTVTSCKHEYHLQCIIEWSQRSKECPIC 86

[Close]
BLAST of Carg20991 vs. TrEMBL
Match: tr|A0A1S3AVH0|A0A1S3AVH0_CUCME (E3 ubiquitin-protein ligase At3g02290 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483261 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-65
Identity = 128/166 (77.11%), Postives = 138/166 (83.13%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEER +SEI+ GS+SMLL AGHDLNLD SIPNTYR+PPTPLPYD  LK PQ KD NS KE
Sbjct: 26  MEERMTSEINSGSSSMLLNAGHDLNLDFSIPNTYRSPPTPLPYDVVLKYPQQKDPNSAKE 85

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKG-NTPCPVTEE-EDVCPI 120
             CGCS KT+SA+K VGELD K QDS    GPPRKL+HSKSKG +T  PVTEE +DVCPI
Sbjct: 86  RICGCSLKTTSAVKSVGELDRKSQDS----GPPRKLEHSKSKGTSTTTPVTEEDQDVCPI 145

Query: 121 CLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFEL 165
           CLEEY S HPEIITKCKHHFHLACLLEWTERS+ CPICDKE IFEL
Sbjct: 146 CLEEYDSVHPEIITKCKHHFHLACLLEWTERSDVCPICDKEMIFEL 187

[Close]
BLAST of Carg20991 vs. TrEMBL
Match: tr|A0A1S4DSJ0|A0A1S4DSJ0_CUCME (E3 ubiquitin-protein ligase At3g02290 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483261 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.2e-63
Identity = 128/173 (73.99%), Postives = 138/173 (79.77%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEER +SEI+ GS+SMLL AGHDLNLD SIPNTYR+PPTPLPYD  LK PQ KD NS KE
Sbjct: 26  MEERMTSEINSGSSSMLLNAGHDLNLDFSIPNTYRSPPTPLPYDVVLKYPQQKDPNSAKE 85

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKG-NTPCPVTEE-EDVCPI 120
             CGCS KT+SA+K VGELD K QDS    GPPRKL+HSKSKG +T  PVTEE +DVCPI
Sbjct: 86  RICGCSLKTTSAVKSVGELDRKSQDS----GPPRKLEHSKSKGTSTTTPVTEEDQDVCPI 145

Query: 121 CLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDK-------ETIFEL 165
           CLEEY S HPEIITKCKHHFHLACLLEWTERS+ CPICDK       E IFEL
Sbjct: 146 CLEEYDSVHPEIITKCKHHFHLACLLEWTERSDVCPICDKVCSNKLQEMIFEL 194

[Close]
BLAST of Carg20991 vs. TrEMBL
Match: tr|A0A1S3AW03|A0A1S3AW03_CUCME (E3 ubiquitin-protein ligase At3g02290 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103483261 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.2e-63
Identity = 128/173 (73.99%), Postives = 138/173 (79.77%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEER +SEI+ GS+SMLL AGHDLNLD SIPNTYR+PPTPLPYD  LK PQ KD NS KE
Sbjct: 1   MEERMTSEINSGSSSMLLNAGHDLNLDFSIPNTYRSPPTPLPYDVVLKYPQQKDPNSAKE 60

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKG-NTPCPVTEE-EDVCPI 120
             CGCS KT+SA+K VGELD K QDS    GPPRKL+HSKSKG +T  PVTEE +DVCPI
Sbjct: 61  RICGCSLKTTSAVKSVGELDRKSQDS----GPPRKLEHSKSKGTSTTTPVTEEDQDVCPI 120

Query: 121 CLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDK-------ETIFEL 165
           CLEEY S HPEIITKCKHHFHLACLLEWTERS+ CPICDK       E IFEL
Sbjct: 121 CLEEYDSVHPEIITKCKHHFHLACLLEWTERSDVCPICDKVCSNKLQEMIFEL 169

[Close]
BLAST of Carg20991 vs. TrEMBL
Match: tr|A0A1S3AVC9|A0A1S3AVC9_CUCME (E3 ubiquitin-protein ligase At3g02290 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103483261 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.2e-63
Identity = 128/173 (73.99%), Postives = 138/173 (79.77%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHDLNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFKE 60
           MEER +SEI+ GS+SMLL AGHDLNLD SIPNTYR+PPTPLPYD  LK PQ KD NS KE
Sbjct: 19  MEERMTSEINSGSSSMLLNAGHDLNLDFSIPNTYRSPPTPLPYDVVLKYPQQKDPNSAKE 78

Query: 61  TKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKG-NTPCPVTEE-EDVCPI 120
             CGCS KT+SA+K VGELD K QDS    GPPRKL+HSKSKG +T  PVTEE +DVCPI
Sbjct: 79  RICGCSLKTTSAVKSVGELDRKSQDS----GPPRKLEHSKSKGTSTTTPVTEEDQDVCPI 138

Query: 121 CLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDK-------ETIFEL 165
           CLEEY S HPEIITKCKHHFHLACLLEWTERS+ CPICDK       E IFEL
Sbjct: 139 CLEEYDSVHPEIITKCKHHFHLACLLEWTERSDVCPICDKVCSNKLQEMIFEL 187

[Close]
BLAST of Carg20991 vs. TrEMBL
Match: tr|A0A0A0L481|A0A0A0L481_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121780 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-58
Identity = 120/167 (71.86%), Postives = 132/167 (79.04%), Query Frame = 0 

Query: 1   MEERESSEIHGGSASMLLGAGHD-LNLDLSIPNTYRAPPTPLPYDAALKCPQPKDLNSFK 60
           MEER +SEI+ GS+SMLL AGHD LNLD SIP+TYR+PP PLPYD  L  P  KD NS K
Sbjct: 26  MEERMTSEINSGSSSMLLNAGHDHLNLDFSIPSTYRSPPIPLPYDVVLTYPHQKDPNSAK 85

Query: 61  ETKCGCSFKTSSALKIVGELDCKFQDSPLLGGPPRKLDHSKSKGNT-PCPVTEE-EDVCP 120
           E  C CS KT+SA+K VGELD K Q+S    GPP KL+HSKSKGN+   PVTEE +D CP
Sbjct: 86  ERICECSLKTTSAVKSVGELDRKSQES----GPPGKLEHSKSKGNSMTTPVTEEDQDDCP 145

Query: 121 ICLEEYGSEHPEIITKCKHHFHLACLLEWTERSNACPICDKETIFEL 165
           ICLEEY S HPEIITKCKHHFHLACLLEWTERS+ CPICDKE IFEL
Sbjct: 146 ICLEEYDSVHPEIITKCKHHFHLACLLEWTERSDVCPICDKEMIFEL 188

The following BLAST results are available for this feature:
BLAST of Carg20991 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022980128.12.8e-9499.39probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima] >XP_022980129.1 pr... [more]
XP_022924496.11.4e-9398.18probable E3 ubiquitin-protein ligase RHB1A [Cucurbita moschata] >XP_022924497.1 ... [more]
XP_023527676.13.8e-9196.36probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_008438020.12.7e-6577.11PREDICTED: E3 ubiquitin-protein ligase At3g02290 isoform X2 [Cucumis melo][more]
XP_022147277.12.3e-6475.76probable E3 ubiquitin-protein ligase RHB1A [Momordica charantia][more]
BLAST of Carg20991 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G00335.12.3e-3448.37RING-H2 finger B1A[more]
AT5G41350.13.7e-2440.37RING/U-box superfamily protein[more]
AT5G38895.31.1e-2036.49RING/U-box superfamily protein[more]
AT5G15790.13.2e-2035.06RING/U-box superfamily protein[more]
AT3G02290.13.0e-1866.00RING/U-box superfamily protein[more]
BLAST of Carg20991 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q2HIJ8|RHB1A_ARATH4.2e-3348.37Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
sp|Q8LE94|RING3_ARATH5.5e-1766.00E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022... [more]
sp|Q93ZF6|AIRP1_ARATH3.0e-1562.75E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ... [more]
sp|Q9ZT42|RHF2A_ARATH1.1e-0950.00E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ... [more]
sp|Q4TU14|RHF1A_ARATH3.2e-0951.22E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ... [more]
BLAST of Carg20991 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3AVH0|A0A1S3AVH0_CUCME1.8e-6577.11E3 ubiquitin-protein ligase At3g02290 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A1S4DSJ0|A0A1S4DSJ0_CUCME2.2e-6373.99E3 ubiquitin-protein ligase At3g02290 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A1S3AW03|A0A1S3AW03_CUCME2.2e-6373.99E3 ubiquitin-protein ligase At3g02290 isoform X4 OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A1S3AVC9|A0A1S3AVC9_CUCME2.2e-6373.99E3 ubiquitin-protein ligase At3g02290 isoform X3 OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A0A0L481|A0A0A0L481_CUCSA1.2e-5871.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G121780 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20991-RACarg20991-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 116..156
e-value: 1.5E-6
score: 37.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 115..156
e-value: 2.6E-12
score: 46.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 116..157
score: 12.46
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 94..162
e-value: 1.9E-14
score: 54.6
NoneNo IPR availablePANTHERPTHR22937RING FINGER CONTAINING PROTEINcoord: 2..163
NoneNo IPR availablePANTHERPTHR22937:SF24C3H2C3-TYPE RING E3 UB LIGASE-RELATEDcoord: 2..163
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 110..162

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg20991Cla021226Watermelon (97103) v1carwmB0487
Carg20991Csa3G121780Cucumber (Chinese Long) v2carcuB0522
Carg20991MELO3C006098Melon (DHL92) v3.5.1carmeB0497
Carg20991ClCG05G001420Watermelon (Charleston Gray)carwcgB0474
Carg20991CSPI03G08670Wild cucumber (PI 183967)carcpiB0529
Carg20991Cucsa.258490Cucumber (Gy14) v1carcgyB0564
Carg20991CmaCh14G020470Cucurbita maxima (Rimu)carcmaB0683
Carg20991CmoCh14G021110Cucurbita moschata (Rifu)carcmoB0664
Carg20991Lsi05G020500Bottle gourd (USVL1VR-Ls)carlsiB445
Carg20991Cp4.1LG03g16640Cucurbita pepo (Zucchini)carcpeB0691
Carg20991CsGy3G008750Cucumber (Gy14) v2carcgybB0499
Carg20991MELO3C006098.2Melon (DHL92) v3.6.1carmedB0503
Carg20991CsaV3_3G008810Cucumber (Chinese Long) v3carcucB0528
Carg20991Cla97C05G081780Watermelon (97103) v2carwmbB0513
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg20991Silver-seed gourdcarcarB409
Carg20991Cucumber (Gy14) v1carcgyB0563
Carg20991Cucurbita maxima (Rimu)carcmaB0684
Carg20991Cucurbita moschata (Rifu)carcmoB0665
Carg20991Cucurbita moschata (Rifu)carcmoB0666
Carg20991Cucurbita pepo (Zucchini)carcpeB0692
Carg20991Cucurbita pepo (Zucchini)carcpeB0690
Carg20991Cucurbita pepo (Zucchini)carcpeB0693
Carg20991Wild cucumber (PI 183967)carcpiB0530
Carg20991Bottle gourd (USVL1VR-Ls)carlsiB444
Carg20991Melon (DHL92) v3.5.1carmeB0496
Carg20991Melon (DHL92) v3.5.1carmeB0498
Carg20991Melon (DHL92) v3.6.1carmedB0502
Carg20991Watermelon (Charleston Gray)carwcgB0475
Carg20991Watermelon (Charleston Gray)carwcgB0476
Carg20991Watermelon (97103) v1carwmB0488
Carg20991Wax gourdcarwgoB0594