Carg20797 (gene) Silver-seed gourd

NameCarg20797
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionWAT1-related protein
LocationCucurbita_argyrosperma_scaffold_153 : 28414 .. 30463 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGGGCGGGAGTGTCAATGAGGAAGCTGTATGCGCCGTGTGCAGCAATGGTGTTGGTGTATATTGCGTATGGCGGATCCCACATTCTGGTGAAGATCGCTAGCGACAAGGGTCTTAACCCTTTGGTCTTCATCGTTTATCGCCATTTCATAGCCTTCCTCGTCTTGGCTCCTTTTGCTTATCTCATCGACAGGTGCGACCAAAATTGTTTGCTTCTTATATCATTTTCTTGGCTTAATGGTTGATTACATGGTGCCTTTGTATAGAAACAAACCAACGTCTCTCACGATTTCGATGACGATAAACATCTTTTTGCTCGCAATCTTAGGCTCAACGATTCACCTGAATCTATTCTACGCTGGAATTAGCTATACTTCGCCTATAGTAACTTCTACTTTCAGCAACATCATTCCAAGCTTGACATTTGTCATGGCTGTTCTATTTCGGTACTGTACCTATTCTTTTTTCTGTATAGATTAATAAAAATATTCATAAATTTTATACTTTTATCTCGAAAGATTGGAGAGAGTGAATATCAAAACTGCGAGAGGTGGTGCTAAAGTGTTGGGGACGATTATGTGCATCGGTGGCTCCTTTGTTTTCACGTTTTGGAAAGGACCATATGTAACCAAAGGCATCTTCAAGGAGCCACTCATAGACGTGTACAAGAATCAAGGCTGTGGACATAGGAGTGGCGAGGATTGGATAAAGGGCTCGGCTTTGATTGTTGTAAGTGAGGTTGCTTGGAGTGCATGGCTAATTTTATTGGTAAGTTCATTCAAAGTCTTCTTTTATGCAAAGAAATGTGTTGCGACACAATCTGCTCATCCAATCTTAATGGCTTGCAGACATTGGTCACTAGAAAATACCCAGCACAATTGACCATTACTGTTTTAATTTGCTTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGATCTTTGCTAGAGATTTAGAAAAATGGAAGCTACACTGGGATGCACGGCTGTTAACCATCGTATATTGTGTAAGTATAGTCGATTGATTTGTTTCTTGTCCTTATATTTATTTATTCTACCCGGACCTATTCCAAATAAGCTAGGAAATTAGTGTTTAAATGGAGAAGGAGAGAAGTATGTGAGAGGTTTCTCCCCACTAGATAAACACAGCTGACGTCTCCGTCCGTGCCCAATTCCCTATTCTACCGGAATCGTTACAACTTTTTTTACATATAACAATAAAAAATATATTTTTCTATTATAAATTATTATAATTTTTTTTTTAATTTGTTTTGTGTTACCATTACATTTCCATGACATTTTACAAATTATATTTTATTTTGGCTAATAAGTCTTAAAGTTTGTGAAACATCAATATTAATAATATTTATACAAGGTAAACTCTTTCCATGTAACAGTTTAAACTCACTGCTAACAGATATTATGTGTTTTTGCATATTAGGTGTCGTCATTACCCTCATAATTTTAAAAGTGCCTACAAGATAGAGGTTTCGACGCGCGTACAAGAAAGGTTTTGTTTTCCTTGTAGGCCAACAATCTCGCTGACACACCGCTCGATGCTTGACTTTGATATAGCTTCTTGATTTGTGTGTGTTGACGTGTTTTAAAATCGTGAGGCTGACAGTACCACGTAACAAACTAAAACGAATAATATATGTTAGCAGTAGACTTGAACTGTTATAGTTTATCACTCTCAAATCCCTTCTTAAATCATCTACTCTCCTATTTGTATATAGGGCGTTGTGATTTCGGGCGCGGGGTATTTCCTACAAACTTGGTGCATCAGCCGCAACGGGCCTGTTTTTACTTCAATGTTTAATCCCCTAATGCTGATATTCGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCACGTGGGCAGGTAAGCCACTCACTTCCTACTATGTTTGTATTGAATTGAAGGTGTTGCCTGAAACTGTTGCGTTATGAAGCCTGGTAGGAGCTTTCCTTATCATACTGGGTCTGTACTTGGTGCTGTGGGGTAAGAAGGCAGATCATGAGGTTGCACCTGAACCGTAG

mRNA sequence

ATGGCAAGGGCGGGAGTGTCAATGAGGAAGCTGTATGCGCCGTGTGCAGCAATGGTGTTGGTGTATATTGCGTATGGCGGATCCCACATTCTGGTGAAGATCGCTAGCGACAAGGGTCTTAACCCTTTGGTCTTCATCGTTTATCGCCATTTCATAGCCTTCCTCGTCTTGGCTCCTTTTGCTTATCTCATCGACAGAAACAAACCAACGTCTCTCACGATTTCGATGACGATAAACATCTTTTTGCTCGCAATCTTAGGCTCAACGATTCACCTGAATCTATTCTACGCTGGAATTAGCTATACTTCGCCTATAGTAACTTCTACTTTCAGCAACATCATTCCAAGCTTGACATTTGTCATGGCTGTTCTATTTCGATTGGAGAGAGTGAATATCAAAACTGCGAGAGGTGGTGCTAAAGTGTTGGGGACGATTATGTGCATCGGTGGCTCCTTTGTTTTCACGTTTTGGAAAGGACCATATGTAACCAAAGGCATCTTCAAGGAGCCACTCATAGACGTGTACAAGAATCAAGGCTGTGGACATAGGAGTGGCGAGGATTGGATAAAGGGCTCGGCTTTGATTGTTGTAAGTGAGGTTGCTTGGAGTGCATGGCTAATTTTATTGACATTGGTCACTAGAAAATACCCAGCACAATTGACCATTACTGTTTTAATTTGCTTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGATCTTTGCTAGAGATTTAGAAAAATGGAAGCTACACTGGGATGCACGGCTGTTAACCATCGTATATTGTGGCGTTGTGATTTCGGGCGCGGGGTATTTCCTACAAACTTGGTGCATCAGCCGCAACGGGCCTGTTTTTACTTCAATGTTTAATCCCCTAATGCTGATATTCGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCACGTGGGCAGCCTGGTAGGAGCTTTCCTTATCATACTGGGTCTGTACTTGGTGCTGTGGGGTAAGAAGGCAGATCATGAGGTTGCACCTGAACCGTAG

Coding sequence (CDS)

ATGGCAAGGGCGGGAGTGTCAATGAGGAAGCTGTATGCGCCGTGTGCAGCAATGGTGTTGGTGTATATTGCGTATGGCGGATCCCACATTCTGGTGAAGATCGCTAGCGACAAGGGTCTTAACCCTTTGGTCTTCATCGTTTATCGCCATTTCATAGCCTTCCTCGTCTTGGCTCCTTTTGCTTATCTCATCGACAGAAACAAACCAACGTCTCTCACGATTTCGATGACGATAAACATCTTTTTGCTCGCAATCTTAGGCTCAACGATTCACCTGAATCTATTCTACGCTGGAATTAGCTATACTTCGCCTATAGTAACTTCTACTTTCAGCAACATCATTCCAAGCTTGACATTTGTCATGGCTGTTCTATTTCGATTGGAGAGAGTGAATATCAAAACTGCGAGAGGTGGTGCTAAAGTGTTGGGGACGATTATGTGCATCGGTGGCTCCTTTGTTTTCACGTTTTGGAAAGGACCATATGTAACCAAAGGCATCTTCAAGGAGCCACTCATAGACGTGTACAAGAATCAAGGCTGTGGACATAGGAGTGGCGAGGATTGGATAAAGGGCTCGGCTTTGATTGTTGTAAGTGAGGTTGCTTGGAGTGCATGGCTAATTTTATTGACATTGGTCACTAGAAAATACCCAGCACAATTGACCATTACTGTTTTAATTTGCTTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGATCTTTGCTAGAGATTTAGAAAAATGGAAGCTACACTGGGATGCACGGCTGTTAACCATCGTATATTGTGGCGTTGTGATTTCGGGCGCGGGGTATTTCCTACAAACTTGGTGCATCAGCCGCAACGGGCCTGTTTTTACTTCAATGTTTAATCCCCTAATGCTGATATTCGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCACGTGGGCAGCCTGGTAGGAGCTTTCCTTATCATACTGGGTCTGTACTTGGTGCTGTGGGGTAAGAAGGCAGATCATGAGGTTGCACCTGAACCGTAG

Protein sequence

MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP
BLAST of Carg20797 vs. NCBI nr
Match: XP_022928963.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 659.1 bits (1699), Expect = 8.4e-186
Identity = 335/341 (98.24%), Postives = 338/341 (99.12%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           MARAGVSMRKLY PCAAMVLVYIAYGGSHILVKIAS+KGLNPLVFIVYRHFIAFLVLAPF
Sbjct: 1   MARAGVSMRKLYGPCAAMVLVYIAYGGSHILVKIASNKGLNPLVFIVYRHFIAFLVLAPF 60

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTF+NIIPSLTFV
Sbjct: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFNNIIPSLTFV 120

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180
           MA LFRLERVNI+TARGGAKVLGTI CIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC
Sbjct: 121 MAFLFRLERVNIRTARGGAKVLGTITCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180

Query: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240
           GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI
Sbjct: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240

Query: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300
           FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 341

BLAST of Carg20797 vs. NCBI nr
Match: XP_023520568.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo] >XP_023521475.1 WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.0 bits (1647), Expect = 9.0e-180
Identity = 326/341 (95.60%), Postives = 332/341 (97.36%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           MA AGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF
Sbjct: 1   MATAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AYLIDRNK TSLT+SMTINIFLLAILGSTIHLNLFYAGISYTSPIVT+TFSNIIPSLTFV
Sbjct: 61  AYLIDRNKRTSLTLSMTINIFLLAILGSTIHLNLFYAGISYTSPIVTTTFSNIIPSLTFV 120

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180
           MAVLFRLERVNIKTARGGAKVLGTI+CIGGSFVFTFWKGPYVTKG+FKEPLIDVYKNQG 
Sbjct: 121 MAVLFRLERVNIKTARGGAKVLGTIVCIGGSFVFTFWKGPYVTKGMFKEPLIDVYKNQGS 180

Query: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240
           GH SGEDWIKGS LI+VSEVAWSAWLILL LVTRKYPAQLTITVL+CLFATAQSGFLALI
Sbjct: 181 GHSSGEDWIKGSGLILVSEVAWSAWLILLALVTRKYPAQLTITVLMCLFATAQSGFLALI 240

Query: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300
           FARDLEKWKL WDARLLTIVYCGVVISG GYFLQTWCISRNGPVFTSMFNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLQWDARLLTIVYCGVVISGVGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVA EP
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAAEP 341

BLAST of Carg20797 vs. NCBI nr
Match: XP_022928958.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 552.0 bits (1421), Expect = 1.4e-153
Identity = 275/334 (82.34%), Postives = 299/334 (89.52%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV+V I YGGS+IL+KIAS+K LNP VF+VYRHFIAFL+LAPFAY+IDRN
Sbjct: 1   MAKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           KP SLTIS  I IFLLAILGSTIHLNLFY GI YTSP V S FSN+IPSLTFVMAVLFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNI+TARG AKV+GTI+CIGGS +FT WKGPYVTK IFKEPLID+YKN   G+ SG+D
Sbjct: 121 EKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA I++S++AWS WLIL   VTRKYPAQLT+TVLICLFATAQSGFLALIFARD EK
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKL WDARLLTIVY G+VISG GYFLQTWCISRNGPVFTSMF+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA EP
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEP 334

BLAST of Carg20797 vs. NCBI nr
Match: XP_022970063.1 (WAT1-related protein At1g43650-like [Cucurbita maxima])

HSP 1 Score: 550.1 bits (1416), Expect = 5.5e-153
Identity = 274/334 (82.04%), Postives = 298/334 (89.22%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV+V I YGGS+IL+KIAS+K LNP VF+VYRHFIAFL+LAPFAY+IDRN
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           KP SLTIS  I IFLLAILGSTIHLNLFY GI YTSP V S FSN+IPSLTFVMAVLFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNI+TARG AKV GTI+CIGGS +FT WKGPYVTK IFKEPLID+Y+N   G+ SG+D
Sbjct: 121 EKVNIRTARGAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA I++S++AWS WLIL   VTRKYPAQLT+TVLICLFATAQSGFLALIFARD EK
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKL WDARLLTIVY G+VISG GYFLQTWCISRNGPVFTSMF+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA EP
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEP 334

BLAST of Carg20797 vs. NCBI nr
Match: XP_023520560.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546.2 bits (1406), Expect = 7.9e-152
Identity = 272/334 (81.44%), Postives = 296/334 (88.62%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV+V I YGGS+IL+KIAS+K LNP VF+VYRHFIAFL+LAPFAY IDRN
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYFIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           KP SLTI   I IFLLAILGSTIHLNLFY GI YTSP V S FSN+IPSLTFVMAVLFRL
Sbjct: 61  KPASLTIPTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNI++A GGAKVLGTI+CIGGS +FT WKGPYVTKG+FKEPLID+YKN   G+ SG+D
Sbjct: 121 EKVNIRSAGGGAKVLGTIVCIGGSLIFTLWKGPYVTKGMFKEPLIDIYKNHDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA I++S++AWS WLIL   VTRKYPAQLT+TVLIC FATAQSGFLALIFARD EK
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICFFATAQSGFLALIFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKL WDARLLTIVY G+VISG GYFLQTWCIS NGPVFTSMF+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISHNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA EP
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEP 334

BLAST of Carg20797 vs. TAIR10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 239.2 bits (609), Expect = 3.7e-63
Identity = 126/324 (38.89%), Postives = 198/324 (61.11%), Query Frame = 0

Query: 17  AMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISM 76
           AMV V I Y G  +L K+A  +G NP VF+ YR   A L L+PFA+ ++ +K + L+  +
Sbjct: 10  AMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFIL 69

Query: 77  TINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTAR 136
            + IF +++ G T+ LNL+Y  I  T+    +  +N IPS+TFV+A+LFRLE V +K + 
Sbjct: 70  LLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLETVTLKKSH 129

Query: 137 GGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGH---RSGEDWIKGSA 196
           G AKV G+++ + G+ VF F KGP          LI+ Y +    +    S ++ +KGS 
Sbjct: 130 GVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPSTKNSVKGSI 189

Query: 197 LIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWD 256
            ++ +   W  W+I+ + V ++YPA+L +  L CLF+  QS   A+   R+   WK+ + 
Sbjct: 190 TMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFG 249

Query: 257 ARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGS 316
             LL++ YCG++++G  Y+LQ W I + GPVFT+++ PL LI   I S+F+F E  ++GS
Sbjct: 250 LPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGS 309

Query: 317 LVGAFLIILGLYLVLWGKKADHEV 338
           + GA L++ GLYL LWGK  + E+
Sbjct: 310 VGGAVLLVCGLYLGLWGKTKEEEI 324

BLAST of Carg20797 vs. TAIR10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 234.6 bits (597), Expect = 9.2e-62
Identity = 115/329 (34.95%), Postives = 199/329 (60.49%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P  AM+ +   Y G +I+ KI+ + G++  V +VYRH IA  V+APFA+  +R     +T
Sbjct: 18  PYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKIT 77

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
            S+ + +F+L +LG  I  N +Y G+ YTSP  +   SN++P++TF++AVLFR+E +++K
Sbjct: 78  FSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLK 137

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYV----TKGIFKEPLIDVYKNQGCGHRSGEDWI 193
                AK+ GT++ + G+ + T +KGP V    TK +  +              S ++++
Sbjct: 138 KLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFL 197

Query: 194 KGSALIVVSEVAWSAWLILLTLVTRKYPA-QLTITVLICLFATAQSGFLALIFARDLEKW 253
           KGS L++ + +AW++  +L   + + Y   QL++T LIC   T Q+  +  +   +   W
Sbjct: 198 KGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAW 257

Query: 254 KLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSER 313
           ++ WD  LL   Y G+V S   Y++Q   + + GPVF + F+PLM++ VA+  +FV +E+
Sbjct: 258 RIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEK 317

Query: 314 LHVGSLVGAFLIILGLYLVLWGKKADHEV 338
           + +G ++GA LI++GLY VLWGK+ +++V
Sbjct: 318 IFLGGVIGAVLIVIGLYAVLWGKQKENQV 346

BLAST of Carg20797 vs. TAIR10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 233.8 bits (595), Expect = 1.6e-61
Identity = 119/332 (35.84%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P   + ++ + Y    ++ K   + G+N  VF+ YR   A + LAP A+  +R     L+
Sbjct: 8   PYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPLS 67

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
               I IF+L++ G T+ L+L    +SYTS  + +  +  +P++TF +A+LF +ER+ +K
Sbjct: 68  FVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKVK 127

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLI-DVYKNQGCGHRS-------- 193
           + +G AK++G  +C+GG  +   +KGP     + K PL    Y  Q   HR+        
Sbjct: 128 SIQGTAKLVGITVCMGGVIILAIYKGP-----LLKLPLCPHFYHGQEHPHRNNPGHVSGG 187

Query: 194 GEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARD 253
              W+KG  L++ S + W  WL+L   V + YP++L  T L CL ++ QS  +A+   RD
Sbjct: 188 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 247

Query: 254 LEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFV 313
           +  WKL W+ RL+ ++YCG +++G  Y+LQ+W I + GPVF SMF PL L+F  + SA +
Sbjct: 248 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 307

Query: 314 FSERLHVGSLVGAFLIILGLYLVLWGKKADHE 337
             E + +GS+VG  L+I+GLY VLWGK  + +
Sbjct: 308 LCEIISLGSIVGGLLLIIGLYCVLWGKSREEK 334

BLAST of Carg20797 vs. TAIR10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 231.1 bits (588), Expect = 1.0e-60
Identity = 121/328 (36.89%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 10  KLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKP 69
           K + P   +V +   Y G  I+ K A ++G++P V   YRH +A + +APFAY +DR   
Sbjct: 4   KTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIR 63

Query: 70  TSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLER 129
             +T+S+   I LL +L  TI  NL+Y G+ YTS   T+  +N++P+  F+MA +FRLE+
Sbjct: 64  PKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEK 123

Query: 130 VNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSG--ED 189
           VN+K     AK+LGTI+ +GG+ + T  KGP +       P  + +        +G  +D
Sbjct: 124 VNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPL-----PWANPHDIHQDSSNTGVKQD 183

Query: 190 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFAR-DLE 249
             KG++LI +  + W+ ++ L  +  + YP +L++T  IC   + +S  +AL   R +  
Sbjct: 184 LTKGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPS 243

Query: 250 KWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFS 309
            W +H D++LL  VY GV+ SG GY++Q   +   GPVF + FNPL ++ VAI  + + +
Sbjct: 244 AWAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILA 303

Query: 310 ERLHVGSLVGAFLIILGLYLVLWGKKAD 335
           E + +G ++GA +I+LGLY VLWGK  D
Sbjct: 304 EVMFLGRILGAIVIVLGLYSVLWGKSKD 326

BLAST of Carg20797 vs. TAIR10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 230.7 bits (587), Expect = 1.3e-60
Identity = 119/338 (35.21%), Postives = 194/338 (57.40%), Query Frame = 0

Query: 5   GVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLI 64
           G  M KL  P  A++ +   Y G +I+  ++   G+N  +   YRH +A +V+APFA ++
Sbjct: 3   GGKMDKL-KPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALIL 62

Query: 65  DRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVL 124
           +R     +T  + + I  L  L   +  NL+Y G+  TS   +S F N +P++TF+MAV+
Sbjct: 63  ERKIRPKMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVI 122

Query: 125 FRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQG--CGH 184
           FR+E VN+K  R  AKV+GT + +GG+ V T +KGP +   +FK     ++         
Sbjct: 123 FRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIE--LFKTAHSSLHGGSSGTSSE 182

Query: 185 RSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFA 244
            + ++W+ G+  ++ S   W+ + IL +   +KYPA+L++ + IC   T  +   +LI  
Sbjct: 183 TTDQNWVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMV 242

Query: 245 RDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSA 304
           RD+  WK+  D+  L  VY GVV SG  Y++Q+  I   GPVFT+ F+P+ +I  A    
Sbjct: 243 RDVSAWKVGMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGV 302

Query: 305 FVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPE 341
            V +E++H+GS++GA  I+ GLY V+WGK  D  ++ E
Sbjct: 303 LVLAEKIHLGSIIGAIFIVFGLYSVVWGKAKDEVISVE 337

BLAST of Carg20797 vs. Swiss-Prot
Match: sp|Q6NMB7|WTR7_ARATH (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.8e-62
Identity = 126/324 (38.89%), Postives = 198/324 (61.11%), Query Frame = 0

Query: 17  AMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISM 76
           AMV V I Y G  +L K+A  +G NP VF+ YR   A L L+PFA+ ++ +K + L+  +
Sbjct: 10  AMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFIL 69

Query: 77  TINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTAR 136
            + IF +++ G T+ LNL+Y  I  T+    +  +N IPS+TFV+A+LFRLE V +K + 
Sbjct: 70  LLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLETVTLKKSH 129

Query: 137 GGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGH---RSGEDWIKGSA 196
           G AKV G+++ + G+ VF F KGP          LI+ Y +    +    S ++ +KGS 
Sbjct: 130 GVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPSTKNSVKGSI 189

Query: 197 LIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWD 256
            ++ +   W  W+I+ + V ++YPA+L +  L CLF+  QS   A+   R+   WK+ + 
Sbjct: 190 TMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFG 249

Query: 257 ARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGS 316
             LL++ YCG++++G  Y+LQ W I + GPVFT+++ PL LI   I S+F+F E  ++GS
Sbjct: 250 LPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGS 309

Query: 317 LVGAFLIILGLYLVLWGKKADHEV 338
           + GA L++ GLYL LWGK  + E+
Sbjct: 310 VGGAVLLVCGLYLGLWGKTKEEEI 324

BLAST of Carg20797 vs. Swiss-Prot
Match: sp|Q9FL41|WTR38_ARATH (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.7e-60
Identity = 115/329 (34.95%), Postives = 199/329 (60.49%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P  AM+ +   Y G +I+ KI+ + G++  V +VYRH IA  V+APFA+  +R     +T
Sbjct: 18  PYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKIT 77

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
            S+ + +F+L +LG  I  N +Y G+ YTSP  +   SN++P++TF++AVLFR+E +++K
Sbjct: 78  FSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLK 137

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYV----TKGIFKEPLIDVYKNQGCGHRSGEDWI 193
                AK+ GT++ + G+ + T +KGP V    TK +  +              S ++++
Sbjct: 138 KLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFL 197

Query: 194 KGSALIVVSEVAWSAWLILLTLVTRKYPA-QLTITVLICLFATAQSGFLALIFARDLEKW 253
           KGS L++ + +AW++  +L   + + Y   QL++T LIC   T Q+  +  +   +   W
Sbjct: 198 KGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAW 257

Query: 254 KLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSER 313
           ++ WD  LL   Y G+V S   Y++Q   + + GPVF + F+PLM++ VA+  +FV +E+
Sbjct: 258 RIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEK 317

Query: 314 LHVGSLVGAFLIILGLYLVLWGKKADHEV 338
           + +G ++GA LI++GLY VLWGK+ +++V
Sbjct: 318 IFLGGVIGAVLIVIGLYAVLWGKQKENQV 346

BLAST of Carg20797 vs. Swiss-Prot
Match: sp|Q9FGG3|WTR45_ARATH (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.8e-60
Identity = 119/332 (35.84%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P   + ++ + Y    ++ K   + G+N  VF+ YR   A + LAP A+  +R     L+
Sbjct: 8   PYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPLS 67

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
               I IF+L++ G T+ L+L    +SYTS  + +  +  +P++TF +A+LF +ER+ +K
Sbjct: 68  FVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKVK 127

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLI-DVYKNQGCGHRS-------- 193
           + +G AK++G  +C+GG  +   +KGP     + K PL    Y  Q   HR+        
Sbjct: 128 SIQGTAKLVGITVCMGGVIILAIYKGP-----LLKLPLCPHFYHGQEHPHRNNPGHVSGG 187

Query: 194 GEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARD 253
              W+KG  L++ S + W  WL+L   V + YP++L  T L CL ++ QS  +A+   RD
Sbjct: 188 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 247

Query: 254 LEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFV 313
           +  WKL W+ RL+ ++YCG +++G  Y+LQ+W I + GPVF SMF PL L+F  + SA +
Sbjct: 248 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 307

Query: 314 FSERLHVGSLVGAFLIILGLYLVLWGKKADHE 337
             E + +GS+VG  L+I+GLY VLWGK  + +
Sbjct: 308 LCEIISLGSIVGGLLLIIGLYCVLWGKSREEK 334

BLAST of Carg20797 vs. Swiss-Prot
Match: sp|Q6J163|5NG4_PINTA (Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.4e-59
Identity = 124/340 (36.47%), Postives = 195/340 (57.35%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           M R  V M +     AAM+ +   Y G HI+ + A + G++ +VF VYR+ +A +++ P 
Sbjct: 6   MQRCNVFMSERVKLHAAMLALQFGYAGFHIVSRAALNMGVSKVVFPVYRNILALMLIGPC 65

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AY +++ +  +LT+S  I  FLLA+ G T    +    I    P   S   N +P++TF+
Sbjct: 66  AYFLEKKERPALTLSFLIQFFLLALCGITGQSRILSLRIVLHIPTFASAIQNSVPAITFI 125

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPL------IDV 180
           MA   RLE+V+I    G AK++GT+ C+ G+ + T +KGP +T  I++  L         
Sbjct: 126 MAAALRLEKVHISRRDGLAKIIGTVACVSGATIITLYKGPPITH-IWRPNLEVTASYFKA 185

Query: 181 YKNQGCGHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQS 240
           ++      +S E+W  G   ++ + +AWS W++L   V ++YPA+L++T   C F   Q 
Sbjct: 186 FQGNDLSAKS-ENWTLGCIYLLGNCLAWSGWIVLQAPVLKRYPARLSVTSFTCFFGVIQF 245

Query: 241 GFLALIFARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLML 300
             +A  F  DLE WK+H    L TI+Y G V SG  + +Q WCI R GPVF +++ P+  
Sbjct: 246 LIIAAFFETDLEHWKIHSGGELFTILYAGFVASGIAFSVQIWCIDRGGPVFVAVYQPVQT 305

Query: 301 IFVAIFSAFVFSERLHVGSLVGAFLIILGLYLVLWGKKAD 335
           I VAI ++ +  E+ ++G + GA LII+GLYLVLWGK  +
Sbjct: 306 IAVAIMASIILGEQFYLGGIFGAILIIIGLYLVLWGKSEE 343

BLAST of Carg20797 vs. Swiss-Prot
Match: sp|O80638|WTR14_ARATH (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.8e-59
Identity = 121/328 (36.89%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 10  KLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKP 69
           K + P   +V +   Y G  I+ K A ++G++P V   YRH +A + +APFAY +DR   
Sbjct: 4   KTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIR 63

Query: 70  TSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLER 129
             +T+S+   I LL +L  TI  NL+Y G+ YTS   T+  +N++P+  F+MA +FRLE+
Sbjct: 64  PKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEK 123

Query: 130 VNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSG--ED 189
           VN+K     AK+LGTI+ +GG+ + T  KGP +       P  + +        +G  +D
Sbjct: 124 VNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPL-----PWANPHDIHQDSSNTGVKQD 183

Query: 190 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFAR-DLE 249
             KG++LI +  + W+ ++ L  +  + YP +L++T  IC   + +S  +AL   R +  
Sbjct: 184 LTKGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPS 243

Query: 250 KWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFS 309
            W +H D++LL  VY GV+ SG GY++Q   +   GPVF + FNPL ++ VAI  + + +
Sbjct: 244 AWAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILA 303

Query: 310 ERLHVGSLVGAFLIILGLYLVLWGKKAD 335
           E + +G ++GA +I+LGLY VLWGK  D
Sbjct: 304 EVMFLGRILGAIVIVLGLYSVLWGKSKD 326

BLAST of Carg20797 vs. TrEMBL
Match: tr|D7TVW3|D7TVW3_VITVI (WAT1-related protein OS=Vitis vinifera OX=29760 GN=VIT_02s0025g03140 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.7e-110
Identity = 198/327 (60.55%), Postives = 256/327 (78.29%), Query Frame = 0

Query: 10  KLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKP 69
           K + P  AMVL+ +AYGGS+IL+KIA DKGLN +VF+VYRH IA L+L PFAY+ +R + 
Sbjct: 2   KNWGPYGAMVLIQLAYGGSNILIKIAIDKGLNQIVFVVYRHIIAMLLLGPFAYVFERTQR 61

Query: 70  TSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLER 129
            SL+ S+ + IF+LA LG+T+HLN++YAG+ YTS  V S   N+IP LTF+MAVLFR+E 
Sbjct: 62  PSLSFSVMMKIFVLASLGTTVHLNVYYAGLHYTSATVASALGNVIPGLTFLMAVLFRMEE 121

Query: 130 VNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCG--HRSGED 189
           V I++ARG AKVLGT++CIGG+  FTFWKG Y+  G  + PLI+VY  +G    H  G++
Sbjct: 122 VRIRSARGRAKVLGTMVCIGGALTFTFWKGGYLFNGFTERPLINVYSTKGSASEHHGGKN 181

Query: 190 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 249
           WIKGS LI++S +AWS+WLIL  +V + YPA+L++ VLIC FA+ QS FLALIFAR+   
Sbjct: 182 WIKGSVLILISHIAWSSWLILQAVVYKVYPARLSLNVLICFFASLQSSFLALIFARNPVL 241

Query: 250 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 309
           WKL W+ +LLTI+YCGVVISG  Y+LQTWCIS+ GPVF +MF PL++IFV IFSA  F+E
Sbjct: 242 WKLEWNVQLLTIIYCGVVISGLVYYLQTWCISKRGPVFAAMFTPLLVIFVGIFSAVAFAE 301

Query: 310 RLHVGSLVGAFLIILGLYLVLWGKKAD 335
           RLH+ SL+GAFLII GLY+VLWGK+ D
Sbjct: 302 RLHLSSLIGAFLIIAGLYIVLWGKRTD 328

BLAST of Carg20797 vs. TrEMBL
Match: tr|B9RYZ0|B9RYZ0_RICCO (WAT1-related protein OS=Ricinus communis OX=3988 GN=RCOM_1314930 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.3e-105
Identity = 194/328 (59.15%), Postives = 251/328 (76.52%), Query Frame = 0

Query: 16  AAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTIS 75
           AAM+LV +AYGGS+IL+KIA +KGLN LVF+VYRH IA +++ PFAY+++R +   L++ 
Sbjct: 8   AAMILVQLAYGGSNILMKIALEKGLNQLVFVVYRHLIAMILVGPFAYVLERKQRPPLSLP 67

Query: 76  MTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTA 135
           +   IF+LA LG+TIHLN++YAG++YTS  V S  SN+IPSLTFVMAVL  +E++ I +A
Sbjct: 68  VITKIFVLASLGTTIHLNVYYAGLAYTSATVASALSNVIPSLTFVMAVLLGMEKLKISSA 127

Query: 136 RGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCG--HRSGEDWIKGSA 195
           RG AKV GT++CIGGS VFTFWKG Y+ K + + PLI +Y ++G      + E+WIKGSA
Sbjct: 128 RGWAKVFGTVICIGGSLVFTFWKGGYLYKSLEERPLISIYSSRGHNELRHAKENWIKGSA 187

Query: 196 LIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWD 255
           LI+ S +AWSAWLIL  +V + YPA+L++T LIC FA+ QS  LAL F R+   WKL W+
Sbjct: 188 LILTSHIAWSAWLILQAVVYKVYPARLSLTTLICFFASIQSSVLALFFGRNPTLWKLDWN 247

Query: 256 ARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGS 315
            +LLTI+YCGVVIS   Y+LQTWCIS+ GPVF +MF+PL+LI V IFSA  F+ERLHVGS
Sbjct: 248 VQLLTIIYCGVVISALVYYLQTWCISKKGPVFAAMFSPLLLIIVGIFSAIAFAERLHVGS 307

Query: 316 LVGAFLIILGLYLVLWGKKADHEVAPEP 342
           L GA LIILGLY VLWGK+ +   A  P
Sbjct: 308 LTGAVLIILGLYCVLWGKRQEDSTAESP 335

BLAST of Carg20797 vs. TrEMBL
Match: tr|A0A2P4JZQ1|A0A2P4JZQ1_QUESU (WAT1-related protein OS=Quercus suber OX=58331 GN=CFP56_07060 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 6.3e-105
Identity = 195/333 (58.56%), Postives = 249/333 (74.77%), Query Frame = 0

Query: 10  KLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKP 69
           K Y    AM +V +AYGG++IL+KIA +KGLN LVF+VYRH IA L+L PFAY+++R   
Sbjct: 2   KSYTSYGAMAIVQLAYGGANILIKIALEKGLNQLVFVVYRHIIAMLLLGPFAYVLERKNR 61

Query: 70  TSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLER 129
             L+ S+   IFLL+ LG+TIHLN++YAG++YTSP V S  SN+IPSLTFV+AV+ R+E 
Sbjct: 62  PPLSFSVIAKIFLLSSLGTTIHLNVYYAGLAYTSPTVASALSNVIPSLTFVIAVILRMET 121

Query: 130 VNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED-W 189
           +NI + RG AKVLGT++CIGG+  FTFWKG Y+ KG    PLI +     C    G+D W
Sbjct: 122 LNIASVRGQAKVLGTLICIGGALTFTFWKGIYLFKGFVHRPLIIICSTNSCELSHGKDNW 181

Query: 190 IKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKW 249
           IKG+ALI+ S +AWSAWLIL  +V + YPA+L++  LIC FA+ QS FLAL FAR+L+ W
Sbjct: 182 IKGAALILTSHIAWSAWLILQAVVYKVYPARLSLNTLICFFASLQSFFLALFFARNLDLW 241

Query: 250 KLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSER 309
           KL W+ +LLTI+Y GVVIS   Y+LQTWCIS  GPVF +MF+PL+LI V IFSA  F+ER
Sbjct: 242 KLEWNMQLLTILYSGVVISALVYYLQTWCISNKGPVFAAMFSPLLLIIVGIFSAIAFAER 301

Query: 310 LHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           LH+GSLVGA LII GLY VLW K+ D+ V  +P
Sbjct: 302 LHLGSLVGASLIIAGLYCVLWAKRTDNLVTGQP 334

BLAST of Carg20797 vs. TrEMBL
Match: tr|A0A2C9WGB9|A0A2C9WGB9_MANES (WAT1-related protein OS=Manihot esculenta OX=3983 GN=MANES_01G000600 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 6.3e-105
Identity = 192/325 (59.08%), Postives = 250/325 (76.92%), Query Frame = 0

Query: 16  AAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTIS 75
           +AM+L+ +AYGGS+IL+KIA DKGLN L F+VYRH IA ++L PFAY+++R +  SL++S
Sbjct: 10  SAMILIQLAYGGSNILIKIALDKGLNQLAFVVYRHVIAVVLLGPFAYVLERKQRPSLSVS 69

Query: 76  MTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTA 135
           +   IF+L+ LG+TIHLN++YAG++YTS  V S  +N+IPSLTF+MAVL R+E+V I TA
Sbjct: 70  VITKIFVLSSLGTTIHLNVYYAGLAYTSATVASALANVIPSLTFLMAVLLRMEKVKISTA 129

Query: 136 RGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGEDWIKGSALI 195
           RG AKVLGT +CIGGS +FTFWKG ++ K   + PLI ++ ++       E+WIKGSALI
Sbjct: 130 RGWAKVLGTTICIGGSLIFTFWKGGFLYKSFEERPLISIHSSKDHAVVK-ENWIKGSALI 189

Query: 196 VVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWDAR 255
           ++S +AWSAWLIL  +V + YPA+L++T LIC FA+ QS FLAL F R+   WKL WD +
Sbjct: 190 LISHIAWSAWLILQAMVCKVYPARLSLTTLICFFASIQSSFLALFFGRNPRLWKLEWDVQ 249

Query: 256 LLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGSLV 315
           LLTI+YCGVVIS   Y+LQTWCIS+ GPVF +MF+PL+LI V IFSA  F+ERLH+GSLV
Sbjct: 250 LLTIIYCGVVISALVYYLQTWCISKKGPVFAAMFSPLLLIIVGIFSAIAFAERLHLGSLV 309

Query: 316 GAFLIILGLYLVLWGKKADHEVAPE 341
           GA  IILGLY  LWGK  D   A +
Sbjct: 310 GAVCIILGLYCALWGKLEDDITAQQ 333

BLAST of Carg20797 vs. TrEMBL
Match: tr|A0A061G1S6|A0A061G1S6_THECC (WAT1-related protein OS=Theobroma cacao OX=3641 GN=TCM_015573 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 8.2e-105
Identity = 196/325 (60.31%), Postives = 250/325 (76.92%), Query Frame = 0

Query: 17  AMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISM 76
           AMV+V  AYGGS+IL+KIA ++GLN  V IVYRH IA L+LAP AY+++R +  SL++S+
Sbjct: 9   AMVVVQFAYGGSNILIKIALERGLNQFVLIVYRHIIAMLLLAPPAYVLERKERPSLSVSV 68

Query: 77  TINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTAR 136
              IFLL+ LG+TIHLN++YAG++YTSP V +   N+IPSLTFVMAVL R+E+V IK+AR
Sbjct: 69  FGKIFLLSSLGTTIHLNVYYAGLAYTSPTVATALGNVIPSLTFVMAVLLRMEKVKIKSAR 128

Query: 137 GGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYK---NQGCGHRSGEDWIKGSA 196
           GGAKVLGT++CIGGS VFTFW+G + +KG    PLI+++    + G      E WIKGSA
Sbjct: 129 GGAKVLGTVICIGGSLVFTFWRGGFQSKGFVDRPLINIHNTNASHGEFRHGNESWIKGSA 188

Query: 197 LIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWD 256
           L +VS +AWS WLIL    ++ YPAQL++ VLIC  A+ QS  LAL FAR+   WKL W+
Sbjct: 189 LFLVSYIAWSGWLILQATASKVYPAQLSLNVLICFIASLQSSVLALFFARNPLLWKLQWN 248

Query: 257 ARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGS 316
            +LLTI+Y GVV+S   Y+LQTWCIS  GPVF +MF PL+L+FVA+FSA VF+ERLH+GS
Sbjct: 249 VQLLTIIYSGVVLSALVYYLQTWCISNKGPVFAAMFTPLLLVFVALFSAIVFAERLHLGS 308

Query: 317 LVGAFLIILGLYLVLWGKKADHEVA 339
           LVGAFLIILGLYLVLW K+ D  V+
Sbjct: 309 LVGAFLIILGLYLVLWAKRRDSFVS 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022928963.18.4e-18698.24WAT1-related protein At1g43650-like [Cucurbita moschata][more]
XP_023520568.19.0e-18095.60WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo] >XP_023521475.1... [more]
XP_022928958.11.4e-15382.34WAT1-related protein At1g43650-like [Cucurbita moschata][more]
XP_022970063.15.5e-15382.04WAT1-related protein At1g43650-like [Cucurbita maxima][more]
XP_023520560.17.9e-15281.44WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G43650.13.7e-6338.89nodulin MtN21 /EamA-like transporter family protein[more]
AT5G07050.19.2e-6234.95nodulin MtN21 /EamA-like transporter family protein[more]
AT5G64700.11.6e-6135.84nodulin MtN21 /EamA-like transporter family protein[more]
AT2G39510.11.0e-6036.89nodulin MtN21 /EamA-like transporter family protein[more]
AT4G08300.11.3e-6035.21nodulin MtN21 /EamA-like transporter family protein[more]
Match NameE-valueIdentityDescription
sp|Q6NMB7|WTR7_ARATH6.8e-6238.89WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2... [more]
sp|Q9FL41|WTR38_ARATH1.7e-6034.95WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
sp|Q9FGG3|WTR45_ARATH2.8e-6035.84WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2... [more]
sp|Q6J163|5NG4_PINTA1.4e-5936.47Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1[more]
sp|O80638|WTR14_ARATH1.8e-5936.89WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Match NameE-valueIdentityDescription
tr|D7TVW3|D7TVW3_VITVI1.7e-11060.55WAT1-related protein OS=Vitis vinifera OX=29760 GN=VIT_02s0025g03140 PE=3 SV=1[more]
tr|B9RYZ0|B9RYZ0_RICCO1.3e-10559.15WAT1-related protein OS=Ricinus communis OX=3988 GN=RCOM_1314930 PE=3 SV=1[more]
tr|A0A2P4JZQ1|A0A2P4JZQ1_QUESU6.3e-10558.56WAT1-related protein OS=Quercus suber OX=58331 GN=CFP56_07060 PE=3 SV=1[more]
tr|A0A2C9WGB9|A0A2C9WGB9_MANES6.3e-10559.08WAT1-related protein OS=Manihot esculenta OX=3983 GN=MANES_01G000600 PE=3 SV=1[more]
tr|A0A061G1S6|A0A061G1S6_THECC8.2e-10560.31WAT1-related protein OS=Theobroma cacao OX=3641 GN=TCM_015573 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR030184WAT1-related
IPR000620EamA_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20797-RACarg20797-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 190..328
e-value: 7.7E-15
score: 55.2
coord: 17..149
e-value: 6.7E-13
score: 48.9
NoneNo IPR availablePANTHERPTHR31218:SF58SUBFAMILY NOT NAMEDcoord: 11..336
NoneNo IPR availableSUPERFAMILYSSF103481Multidrug resistance efflux transporter EmrEcoord: 48..147
NoneNo IPR availableSUPERFAMILYSSF103481Multidrug resistance efflux transporter EmrEcoord: 234..332
IPR030184WAT1-related proteinPANTHERPTHR31218FAMILY NOT NAMEDcoord: 11..336

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None