Carg20412 (gene) Silver-seed gourd

NameCarg20412
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPlant calmodulin-binding-like protein
LocationCucurbita_argyrosperma_scaffold_125 : 260989 .. 263367 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGATGGGAGTATGGATTTGTCAATGTCCCCTGAGACCCACAGATCTGATGGTGATAGGTTGAGAAGATTATCAATGGGAAAAGCAATCTCATTGAGTATTGATGAACAAAACAATTTTCGTGACCGCAGATCCTCAATTGGTTCTTGTCATGATATTTGCAAATATGGACATAATCATTCATTAGAGACGAAGGCCAGAGTTCCCCTGTTGAAAAGAGCAATGAAAAAGGCACTTGATGCCCAAAATTCAGACCAGGCTGTGGTTGTGCCTGAAAAAAAGCATTCTGTTCGTGTGACCAAGTCGAAGGTTTCACCTAGTCCGGGGACATGTATTAGTGGTGGCACTGATGTGATTAAACGGGTAGTGCCCATAAGTTCTCCTAGCAGACGTCCGGTAGAGACTGGAGTTACGAGCAAAAGTAAGGAACAGGCAACGCTTGTAAAAGCTCCTAACAGGCAAAGTGAGACTGAAGTTACAAGCAAAAGTAAGGAACAGGCAACGCTTGTAAAAGCTCCTAATGGGCAAAGTGAGACTGAAGTTACGAGCGAAAGTAAGGAACCGGAGGTGCCTGTGGGTTCTCCTACCAAGCAGATTGAAGATACGAGCGAAAGTAAGGAACCGGAGGTGCCTGTGGGTTCTCCTACCAAGCAGATTGAAGTTATAAGTGAAAATAAGGCACTGGTAGTGCCTATGAATTCTCCTTCCAAGAAGATTGAAGTTATAATGGTACTGCCTGTGAATTCTCCTACCAAGCGGATTGAAGTCATAAGTGAAAGTAAGGAACTGATAGTGCCTGTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTAAGGAACTGGCAGTGCCTTTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTAAGGAACTGGTAGTGCCTTTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTCAGGAACTGGTAGTGCCTGTGAACTCTCCTACCAGGCAAAGTCCAGTAGAGATGGAAGTTTCGAGTGAAAGTAGCGAACGAGTAGTGCCTGAAAATTCTACTAGCAGGCACAGCTCAGTAGAGATTGAAGCTACGAGCAAAAATATGGAACAGGTAGTGCCTGAAACTTCTCCTAGCGGGCGAAGTCGAGTAGAGATTGGAGTTAAGGAACCGGTAGTAGTGGCTGAAAGTTCTCTTAGCAGGCAAGGTTCAAACAGGCGAAGTCCATTAAAGAGTGAAGCTATGAATGAAGGTAAGGAACAGGTGGCCAAGACAAAAACTAAGCCCATAACCTCAAAACCCAAGTTTCATAAAACTACAAAGCAAGTAGTTTATTCGTCTACCAAATCTGAAAGTTCTCCAAAACAGGCTTTGACCAGTGTTGCAGAAGCAGGGGGCGACAGTTTGTCGAAGCCAAAAGCTTTGAAAGCAAAATCAGTGACCTCTTCTGGTCCTTCTGGTAATATTGCAGCCCACAGCAACAACAGTTCTAAATCAGGTGAAGGAGCGGGAACCTTGAAAGGAAATGGAACAAAAGTGGTGGAAAAGTCCATAATCACAACGGATCCAAATTCTGTCGATACCGTGGTAAACTTACCTGCGATAAAGAACAAGAACTCGAAAGTTGTGTCTCAAGTCAAAAGTCAGAACAAAACGAGAAGAGCTCAAGCCAAGGAAGCATCAAGTGTGGAATCACAAGAGAAAATCTTGCATGTCATCAATGTGGAAACTAAGAAGACCAAGCTTGTAGAATCTGACCAAAATGACAAGCATGGTTTGAAGGGGTATCAAAAGTCACCAACATCCTTGGCAAATGGCCCATCTCTTTCACCTCTTAGAGAAGATTCTGGTGGAACTAAATATACAAAATACGAAGCGAATGCCACTGTTTCGGGATCGAAGAAACACGGTGGCATAAAAAAAGAAGATGATCCCAATGGGGTTAAAAAAGGCAAGTCCCCGAGAATGCTTCAGACCAAAGGAAAGGATTCTTCATCTTTCAGCTTAAGTTTCAGGAACAAGAAGGTAATTGACCTTGATTCCAAAAGGCATGGTCCAACGAGGCTCAAATTTATGGAAGGAAAATCGTTGGGGGACAACCAAAAGAGCAAGGATGGCCGAAGAACAAGCTTGAAGAAGGGAATAGTCAAGGGGATTTCCAAGAATTCTACACCACCATCTGAAAAAGTAGTTTTGAGACATCAAGATGTGGAAGGGAAGAAAGATACTCAGGTCTTGTTCAATAATGTCATAGCGGAAACTGCGAGAAAACTCGTTCGTACCCGAAAGAGTAAGGTCAAGGCATTGGTAGGGGCATTTGAAAAGGTTATCTCACTCCAAGACAAGAAGCCTTCTCCTCTAAGAGCCACAGCTTGA

mRNA sequence

ATGGTTGATGGGAGTATGGATTTGTCAATGTCCCCTGAGACCCACAGATCTGATGGTGATAGGTTGAGAAGATTATCAATGGGAAAAGCAATCTCATTGAGTATTGATGAACAAAACAATTTTCGTGACCGCAGATCCTCAATTGGTTCTTGTCATGATATTTGCAAATATGGACATAATCATTCATTAGAGACGAAGGCCAGAGTTCCCCTGTTGAAAAGAGCAATGAAAAAGGCACTTGATGCCCAAAATTCAGACCAGGCTGTGGTTGTGCCTGAAAAAAAGCATTCTGTTCGTGTGACCAAGTCGAAGGTTTCACCTAGTCCGGGGACATGTATTAGTGGTGGCACTGATGTGATTAAACGGGTAGTGCCCATAAGTTCTCCTAGCAGACGTCCGGTAGAGACTGGAGTTACGAGCAAAAGTAAGGAACAGGCAACGCTTGTAAAAGCTCCTAACAGGCAAAGTGAGACTGAAGTTACAAGCAAAAGTAAGGAACAGGCAACGCTTGTAAAAGCTCCTAATGGGCAAAGTGAGACTGAAGTTACGAGCGAAAGTAAGGAACCGGAGGTGCCTGTGGGTTCTCCTACCAAGCAGATTGAAGATACGAGCGAAAGTAAGGAACCGGAGGTGCCTGTGGGTTCTCCTACCAAGCAGATTGAAGTTATAAGTGAAAATAAGGCACTGGTAGTGCCTATGAATTCTCCTTCCAAGAAGATTGAAGTTATAATGGTACTGCCTGTGAATTCTCCTACCAAGCGGATTGAAGTCATAAGTGAAAGTAAGGAACTGATAGTGCCTGTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTAAGGAACTGGCAGTGCCTTTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTAAGGAACTGGTAGTGCCTTTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTCAGGAACTGGTAGTGCCTGTGAACTCTCCTACCAGGCAAAGTCCAGTAGAGATGGAAGTTTCGAGTGAAAGTAGCGAACGAGTAGTGCCTGAAAATTCTACTAGCAGGCACAGCTCAGTAGAGATTGAAGCTACGAGCAAAAATATGGAACAGGTAGTGCCTGAAACTTCTCCTAGCGGGCGAAGTCGAGTAGAGATTGGAGTTAAGGAACCGGTAGTAGTGGCTGAAAGTTCTCTTAGCAGGCAAGGTTCAAACAGGCGAAGTCCATTAAAGAGTGAAGCTATGAATGAAGGTAAGGAACAGGTGGCCAAGACAAAAACTAAGCCCATAACCTCAAAACCCAAGTTTCATAAAACTACAAAGCAAGTAGTTTATTCGTCTACCAAATCTGAAAGTTCTCCAAAACAGGCTTTGACCAGTGTTGCAGAAGCAGGGGGCGACAGTTTGTCGAAGCCAAAAGCTTTGAAAGCAAAATCAGTGACCTCTTCTGGTCCTTCTGGTAATATTGCAGCCCACAGCAACAACAGTTCTAAATCAGGTGAAGGAGCGGGAACCTTGAAAGGAAATGGAACAAAAGTGGTGGAAAAGTCCATAATCACAACGGATCCAAATTCTGTCGATACCGTGGTAAACTTACCTGCGATAAAGAACAAGAACTCGAAAGTTGTGTCTCAAGTCAAAAGTCAGAACAAAACGAGAAGAGCTCAAGCCAAGGAAGCATCAAGTGTGGAATCACAAGAGAAAATCTTGCATGTCATCAATGTGGAAACTAAGAAGACCAAGCTTGTAGAATCTGACCAAAATGACAAGCATGGTTTGAAGGGGTATCAAAAGTCACCAACATCCTTGGCAAATGGCCCATCTCTTTCACCTCTTAGAGAAGATTCTGGTGGAACTAAATATACAAAATACGAAGCGAATGCCACTGTTTCGGGATCGAAGAAACACGGTGGCATAAAAAAAGAAGATGATCCCAATGGGGTTAAAAAAGGCAAGTCCCCGAGAATGCTTCAGACCAAAGGAAAGGATTCTTCATCTTTCAGCTTAAGTTTCAGGAACAAGAAGGTAATTGACCTTGATTCCAAAAGGCATGGTCCAACGAGGCTCAAATTTATGGAAGGAAAATCGTTGGGGGACAACCAAAAGAGCAAGGATGGCCGAAGAACAAGCTTGAAGAAGGGAATAGTCAAGGGGATTTCCAAGAATTCTACACCACCATCTGAAAAAGTAGTTTTGAGACATCAAGATGTGGAAGGGAAGAAAGATACTCAGGTCTTGTTCAATAATGTCATAGCGGAAACTGCGAGAAAACTCGTTCGTACCCGAAAGAGTAAGGTCAAGGCATTGGTAGGGGCATTTGAAAAGGTTATCTCACTCCAAGACAAGAAGCCTTCTCCTCTAAGAGCCACAGCTTGA

Coding sequence (CDS)

ATGGTTGATGGGAGTATGGATTTGTCAATGTCCCCTGAGACCCACAGATCTGATGGTGATAGGTTGAGAAGATTATCAATGGGAAAAGCAATCTCATTGAGTATTGATGAACAAAACAATTTTCGTGACCGCAGATCCTCAATTGGTTCTTGTCATGATATTTGCAAATATGGACATAATCATTCATTAGAGACGAAGGCCAGAGTTCCCCTGTTGAAAAGAGCAATGAAAAAGGCACTTGATGCCCAAAATTCAGACCAGGCTGTGGTTGTGCCTGAAAAAAAGCATTCTGTTCGTGTGACCAAGTCGAAGGTTTCACCTAGTCCGGGGACATGTATTAGTGGTGGCACTGATGTGATTAAACGGGTAGTGCCCATAAGTTCTCCTAGCAGACGTCCGGTAGAGACTGGAGTTACGAGCAAAAGTAAGGAACAGGCAACGCTTGTAAAAGCTCCTAACAGGCAAAGTGAGACTGAAGTTACAAGCAAAAGTAAGGAACAGGCAACGCTTGTAAAAGCTCCTAATGGGCAAAGTGAGACTGAAGTTACGAGCGAAAGTAAGGAACCGGAGGTGCCTGTGGGTTCTCCTACCAAGCAGATTGAAGATACGAGCGAAAGTAAGGAACCGGAGGTGCCTGTGGGTTCTCCTACCAAGCAGATTGAAGTTATAAGTGAAAATAAGGCACTGGTAGTGCCTATGAATTCTCCTTCCAAGAAGATTGAAGTTATAATGGTACTGCCTGTGAATTCTCCTACCAAGCGGATTGAAGTCATAAGTGAAAGTAAGGAACTGATAGTGCCTGTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTAAGGAACTGGCAGTGCCTTTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTAAGGAACTGGTAGTGCCTTTGAATTCTCCTACCAGGCAGATTGAAGTTATAAGTGAAAGTCAGGAACTGGTAGTGCCTGTGAACTCTCCTACCAGGCAAAGTCCAGTAGAGATGGAAGTTTCGAGTGAAAGTAGCGAACGAGTAGTGCCTGAAAATTCTACTAGCAGGCACAGCTCAGTAGAGATTGAAGCTACGAGCAAAAATATGGAACAGGTAGTGCCTGAAACTTCTCCTAGCGGGCGAAGTCGAGTAGAGATTGGAGTTAAGGAACCGGTAGTAGTGGCTGAAAGTTCTCTTAGCAGGCAAGGTTCAAACAGGCGAAGTCCATTAAAGAGTGAAGCTATGAATGAAGGTAAGGAACAGGTGGCCAAGACAAAAACTAAGCCCATAACCTCAAAACCCAAGTTTCATAAAACTACAAAGCAAGTAGTTTATTCGTCTACCAAATCTGAAAGTTCTCCAAAACAGGCTTTGACCAGTGTTGCAGAAGCAGGGGGCGACAGTTTGTCGAAGCCAAAAGCTTTGAAAGCAAAATCAGTGACCTCTTCTGGTCCTTCTGGTAATATTGCAGCCCACAGCAACAACAGTTCTAAATCAGGTGAAGGAGCGGGAACCTTGAAAGGAAATGGAACAAAAGTGGTGGAAAAGTCCATAATCACAACGGATCCAAATTCTGTCGATACCGTGGTAAACTTACCTGCGATAAAGAACAAGAACTCGAAAGTTGTGTCTCAAGTCAAAAGTCAGAACAAAACGAGAAGAGCTCAAGCCAAGGAAGCATCAAGTGTGGAATCACAAGAGAAAATCTTGCATGTCATCAATGTGGAAACTAAGAAGACCAAGCTTGTAGAATCTGACCAAAATGACAAGCATGGTTTGAAGGGGTATCAAAAGTCACCAACATCCTTGGCAAATGGCCCATCTCTTTCACCTCTTAGAGAAGATTCTGGTGGAACTAAATATACAAAATACGAAGCGAATGCCACTGTTTCGGGATCGAAGAAACACGGTGGCATAAAAAAAGAAGATGATCCCAATGGGGTTAAAAAAGGCAAGTCCCCGAGAATGCTTCAGACCAAAGGAAAGGATTCTTCATCTTTCAGCTTAAGTTTCAGGAACAAGAAGGTAATTGACCTTGATTCCAAAAGGCATGGTCCAACGAGGCTCAAATTTATGGAAGGAAAATCGTTGGGGGACAACCAAAAGAGCAAGGATGGCCGAAGAACAAGCTTGAAGAAGGGAATAGTCAAGGGGATTTCCAAGAATTCTACACCACCATCTGAAAAAGTAGTTTTGAGACATCAAGATGTGGAAGGGAAGAAAGATACTCAGGTCTTGTTCAATAATGTCATAGCGGAAACTGCGAGAAAACTCGTTCGTACCCGAAAGAGTAAGGTCAAGGCATTGGTAGGGGCATTTGAAAAGGTTATCTCACTCCAAGACAAGAAGCCTTCTCCTCTAAGAGCCACAGCTTGA

Protein sequence

MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSPGTCISGGTDVIKRVVPISSPSRRPVETGVTSKSKEQATLVKAPNRQSETEVTSKSKEQATLVKAPNGQSETEVTSESKEPEVPVGSPTKQIEDTSESKEPEVPVGSPTKQIEVISENKALVVPMNSPSKKIEVIMVLPVNSPTKRIEVISESKELIVPVNSPTRQIEVISESKELAVPLNSPTRQIEVISESKELVVPLNSPTRQIEVISESQELVVPVNSPTRQSPVEMEVSSESSERVVPENSTSRHSSVEIEATSKNMEQVVPETSPSGRSRVEIGVKEPVVVAESSLSRQGSNRRSPLKSEAMNEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPKALKAKSVTSSGPSGNIAAHSNNSSKSGEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAIKNKNSKVVSQVKSQNKTRRAQAKEASSVESQEKILHVINVETKKTKLVESDQNDKHGLKGYQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEKVISLQDKKPSPLRATA
BLAST of Carg20412 vs. NCBI nr
Match: XP_023523723.1 (uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo] >XP_023523724.1 uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 812.8 bits (2098), Expect = 1.1e-231
Identity = 727/796 (91.33%), Postives = 731/796 (91.83%), Query Frame = 0

Query: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60
           MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQN+FRDRRSSIGSCHDICKYGHN
Sbjct: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHN 60

Query: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSPGTCISGGTDVI 120
           HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPS GTCISGGTDVI
Sbjct: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSSGTCISGGTDVI 120

Query: 121 KRVVPISSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           KRVVPISS                             XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 121 KRVVPISS---------------PSRRPVETGVKSKSXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300
           XXX  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE----XXXXXXXXXXXXXXXXXX 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE    XXXXXXXXXXXXXXXXXX
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVEXXXXXXXXXXXXXXXXXXXXXX 360

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKEPVVVAESSLSRQGSNRRSPLKSEAM 420
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX     VVAESSLSRQGSNRRSPLKSEAM
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVAESSLSRQGSNRRSPLKSEAM 420

Query: 421 NEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPKAL 480
           NEGKE VAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPKAL
Sbjct: 421 NEGKELVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPKAL 480

Query: 481 KAKSVTSSGPSGNIAAHSNNSSKSGEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAI 540
           KAKSVTSSGPSGNIAAHSNN SKSGEGAGTLKGNGTKVVEKSIIT DPNSVDTVVNLPAI
Sbjct: 481 KAKSVTSSGPSGNIAAHSNNISKSGEGAGTLKGNGTKVVEKSIITMDPNSVDTVVNLPAI 540

Query: 541 KNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKG 600
           KNKNSKVVSQVKSQNKTRRAQAK           LHVI        L+ESDQNDKHGLKG
Sbjct: 541 KNKNSKVVSQVKSQNKTRRAQAKEASSVESQEKILHVINVETKKSKLLESDQNDKHGLKG 600

Query: 601 YQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR 660
           YQKSP+SLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR
Sbjct: 601 YQKSPSSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR 660

Query: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI 720
           MLQTKGKDSSSFSLSFRNKKVIDLDSK HGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI
Sbjct: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKSHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI 720

Query: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 780
           VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK
Sbjct: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 780

Query: 781 VISLQDKKPSPLRATA 793
           VISLQDKKP+ LRATA
Sbjct: 781 VISLQDKKPTSLRATA 781

BLAST of Carg20412 vs. NCBI nr
Match: XP_022949115.1 (muscle M-line assembly protein unc-89-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 811.6 bits (2095), Expect = 2.3e-231
Identity = 756/796 (94.97%), Postives = 757/796 (95.10%), Query Frame = 0

Query: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60
           MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN
Sbjct: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60

Query: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSPGTCISGGTDVI 120
           HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPS GTCISGGTDVI
Sbjct: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSSGTCISGGTDVI 120

Query: 121 KRVVPISSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           KRVVPIS                    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 121 KRVVPIS--------------------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300
           XXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 241 XXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE----XXXXXXXXXXXXXXXXXX 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE    XXXXXXXXXXXXXXXXXX
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVEXXXXXXXXXXXXXXXXXXXXXX 360

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKEPVVVAESSLSRQGSNRRSPLKSEAM 420
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX           LSRQGSNRRSPLKSEAM
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSRQGSNRRSPLKSEAM 420

Query: 421 NEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPKAL 480
           NEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQAL SVAEAGGDSLSKPKAL
Sbjct: 421 NEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALASVAEAGGDSLSKPKAL 480

Query: 481 KAKSVTSSGPSGNIAAHSNNSSKSGEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAI 540
           KAKSVTSSGPSGNIAAHSNN+SKS EGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAI
Sbjct: 481 KAKSVTSSGPSGNIAAHSNNNSKSCEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAI 540

Query: 541 KNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKG 600
           KNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKG
Sbjct: 541 KNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKG 600

Query: 601 YQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR 660
           YQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR
Sbjct: 601 YQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR 660

Query: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI 720
           MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFME KSLGDNQKSKDGRRTSLKKGI
Sbjct: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEVKSLGDNQKSKDGRRTSLKKGI 720

Query: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 780
           VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK
Sbjct: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 776

Query: 781 VISLQDKKPSPLRATA 793
           VISLQDKKPSPLRATA
Sbjct: 781 VISLQDKKPSPLRATA 776

BLAST of Carg20412 vs. NCBI nr
Match: XP_022949113.1 (muscle M-line assembly protein unc-89-like isoform X1 [Cucurbita moschata] >XP_022949114.1 muscle M-line assembly protein unc-89-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 810.8 bits (2093), Expect = 4.0e-231
Identity = 775/798 (97.12%), Postives = 776/798 (97.24%), Query Frame = 0

Query: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60
           MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN
Sbjct: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60

Query: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSPGTCISGGTDVI 120
           HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPS GTCISGGTDVI
Sbjct: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSSGTCISGGTDVI 120

Query: 121 KRVVPIS--SXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           KRVVPIS   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 121 KRVVPISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300
           XXXXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 241 XXXXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE----XXXXXXXXXXXXXXXX 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE    XXXXXXXXXXXXXXXX
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVEXXXXXXXXXXXXXXXXXXXX 360

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKEPVVVAESSLSRQGSNRRSPLKSE 420
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX           LSRQGSNRRSPLKSE
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLSRQGSNRRSPLKSE 420

Query: 421 AMNEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPK 480
           AMNEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQAL SVAEAGGDSLSKPK
Sbjct: 421 AMNEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALASVAEAGGDSLSKPK 480

Query: 481 ALKAKSVTSSGPSGNIAAHSNNSSKSGEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLP 540
           ALKAKSVTSSGPSGNIAAHSNN+SKS EGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLP
Sbjct: 481 ALKAKSVTSSGPSGNIAAHSNNNSKSCEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLP 540

Query: 541 AIKNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGL 600
           AIKNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGL
Sbjct: 541 AIKNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGL 600

Query: 601 KGYQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKS 660
           KGYQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKS
Sbjct: 601 KGYQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKS 660

Query: 661 PRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSKDGRRTSLKK 720
           PRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFME KSLGDNQKSKDGRRTSLKK
Sbjct: 661 PRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEVKSLGDNQKSKDGRRTSLKK 720

Query: 721 GIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAF 780
           GIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAF
Sbjct: 721 GIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAF 780

Query: 781 EKVISLQDKKPSPLRATA 793
           EKVISLQDKKPSPLRATA
Sbjct: 781 EKVISLQDKKPSPLRATA 798

BLAST of Carg20412 vs. NCBI nr
Match: XP_022997888.1 (muscle M-line assembly protein unc-89-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 769.2 bits (1985), Expect = 1.3e-218
Identity = 701/796 (88.07%), Postives = 704/796 (88.44%), Query Frame = 0

Query: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60
           MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQN+FRDRRSSIGSCHDICKYGHN
Sbjct: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHN 60

Query: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSPGTCISGGTDVI 120
           HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSV VTKSKVSPS  TCISGGTDVI
Sbjct: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVCVTKSKVSPSSKTCISGGTDVI 120

Query: 121 KRVVPISSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           KRVVPISS                                      XXXXXXXXXXXXXX
Sbjct: 121 KRVVPISS---------------PSRRLVETGVMSKSKEQATLVKAXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300
           XXX  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE----XXXXXXXXXXXXXXXXXX 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE    XXXXXXXXXXXXXXXXXX
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVEXXXXXXXXXXXXXXXXXXXXXX 360

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKEPVVVAESSLSRQGSNRRSPLKSEAM 420
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX          SL RQGSNRRSPLKSEAM
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLRRQGSNRRSPLKSEAM 420

Query: 421 NEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPKAL 480
           NEGKE VAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSL KPKA 
Sbjct: 421 NEGKELVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLLKPKAF 480

Query: 481 KAKSVTSSGPSGNIAAHSNNSSKSGEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAI 540
           K KSV SSG SGNIAAHSNNSSKSGEGAGTLKGNG KVVEKSIIT DPNSVDTVVNLPAI
Sbjct: 481 KEKSVISSGSSGNIAAHSNNSSKSGEGAGTLKGNGMKVVEKSIITMDPNSVDTVVNLPAI 540

Query: 541 KNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKG 600
           K KNSK VSQVKSQNKTRR QAK           LHVI        LVESDQNDKHGLKG
Sbjct: 541 K-KNSKAVSQVKSQNKTRRIQAKEASSVESQEKILHVINVETKKNKLVESDQNDKHGLKG 600

Query: 601 YQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR 660
           YQKSP+SL NGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDD NGVKKGKSPR
Sbjct: 601 YQKSPSSLTNGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDLNGVKKGKSPR 660

Query: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI 720
           MLQTKGKDSSSFSLSFRNKKVIDLDSK HGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI
Sbjct: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKSHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI 720

Query: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 780
           VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK
Sbjct: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 780

Query: 781 VISLQDKKPSPLRATA 793
           VISLQDKKP+ LRATA
Sbjct: 781 VISLQDKKPTSLRATA 780

BLAST of Carg20412 vs. NCBI nr
Match: XP_022997890.1 (muscle M-line assembly protein unc-89-like isoform X4 [Cucurbita maxima])

HSP 1 Score: 769.2 bits (1985), Expect = 1.3e-218
Identity = 701/796 (88.07%), Postives = 704/796 (88.44%), Query Frame = 0

Query: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60
           MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQN+FRDRRSSIGSCHDICKYGHN
Sbjct: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNSFRDRRSSIGSCHDICKYGHN 60

Query: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSPGTCISGGTDVI 120
           HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSV VTKSKVSPS  TCISGGTDVI
Sbjct: 61  HSLETKARVPLLKRAMKKALDAQNSDQAVVVPEKKHSVCVTKSKVSPSSKTCISGGTDVI 120

Query: 121 KRVVPISSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
           KRVVPISS                                      XXXXXXXXXXXXXX
Sbjct: 121 KRVVPISS---------------PSRRLVETGVMSKSKEQATLVKAXXXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240

Query: 241 XXXMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300
           XXX  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE----XXXXXXXXXXXXXXXXXX 360
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVE    XXXXXXXXXXXXXXXXXX
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVEXXXXXXXXXXXXXXXXXXXXXX 360

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKEPVVVAESSLSRQGSNRRSPLKSEAM 420
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX          SL RQGSNRRSPLKSEAM
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSLRRQGSNRRSPLKSEAM 420

Query: 421 NEGKEQVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLSKPKAL 480
           NEGKE VAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSL KPKA 
Sbjct: 421 NEGKELVAKTKTKPITSKPKFHKTTKQVVYSSTKSESSPKQALTSVAEAGGDSLLKPKAF 480

Query: 481 KAKSVTSSGPSGNIAAHSNNSSKSGEGAGTLKGNGTKVVEKSIITTDPNSVDTVVNLPAI 540
           K KSV SSG SGNIAAHSNNSSKSGEGAGTLKGNG KVVEKSIIT DPNSVDTVVNLPAI
Sbjct: 481 KEKSVISSGSSGNIAAHSNNSSKSGEGAGTLKGNGMKVVEKSIITMDPNSVDTVVNLPAI 540

Query: 541 KNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKG 600
           K KNSK VSQVKSQNKTRR QAK           LHVI        LVESDQNDKHGLKG
Sbjct: 541 K-KNSKAVSQVKSQNKTRRIQAKEASSVESQEKILHVINVETKKNKLVESDQNDKHGLKG 600

Query: 601 YQKSPTSLANGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDPNGVKKGKSPR 660
           YQKSP+SL NGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDD NGVKKGKSPR
Sbjct: 601 YQKSPSSLTNGPSLSPLREDSGGTKYTKYEANATVSGSKKHGGIKKEDDLNGVKKGKSPR 660

Query: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI 720
           MLQTKGKDSSSFSLSFRNKKVIDLDSK HGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI
Sbjct: 661 MLQTKGKDSSSFSLSFRNKKVIDLDSKSHGPTRLKFMEGKSLGDNQKSKDGRRTSLKKGI 720

Query: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 780
           VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK
Sbjct: 721 VKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 780

Query: 781 VISLQDKKPSPLRATA 793
           VISLQDKKP+ LRATA
Sbjct: 781 VISLQDKKPTSLRATA 780

BLAST of Carg20412 vs. TAIR10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related)

HSP 1 Score: 86.3 bits (212), Expect = 9.4e-17
Identity = 60/139 (43.17%), Postives = 79/139 (56.83%), Query Frame = 0

Query: 650 KKGKSPRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSL-GDNQKSKDGR 709
           ++GKS           ++  L  R  K+ID  S+ + P +LK      + G +  SK G 
Sbjct: 337 REGKSKAFSAESAITGNAMKLRIRRGKIIDFGSEGNSPRKLKXXXXXIISGADTTSKSGG 396

Query: 710 RTSLKKGIVKGISKNSTPPSEKVVLRHQDVEGKKDTQV-LFNNVIAETARKLVRTRKSKV 769
           R  LK       +        +VVL+HQD E K++++V LFN VI ETA KLV+TRKSKV
Sbjct: 397 RRRLKTKGTNLSNDKEQQRKPRVVLKHQDTEKKRESRVLLFNKVIKETANKLVQTRKSKV 456

Query: 770 KALVGAFEKVISLQDKKPS 787
           KALVGAFE VISLQ+K  S
Sbjct: 457 KALVGAFESVISLQEKTSS 475

BLAST of Carg20412 vs. TAIR10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related)

HSP 1 Score: 76.6 bits (187), Expect = 7.5e-14
Identity = 43/54 (79.63%), Postives = 46/54 (85.19%), Query Frame = 0

Query: 729 EKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEKVISLQD 783
           EKVVLRHQDV+ +KD Q LFNNVI ETA KLV  RKSKVKALVGAFE VISLQ+
Sbjct: 454 EKVVLRHQDVQ-EKDAQGLFNNVIEETASKLVEARKSKVKALVGAFETVISLQE 506


HSP 2 Score: 38.9 bits (89), Expect = 1.7e-02
Identity = 37/117 (31.62%), Postives = 55/117 (47.01%), Query Frame = 0

Query: 10  MSPET--HRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHNHSLETKA 69
           ++PE   ++ DG      S GKA++    E+      R+S GSCHD+CKYG       K 
Sbjct: 10  VTPENMMNKEDGLNPGGDSTGKAMTSKPKEKKIPHYLRASTGSCHDLCKYGKRQIPVEKP 69

Query: 70  RVPLLKRAMKKALDAQNSDQAVVVPEKKHSVRVTKSKVSPSPGTCISGGTDVIKRVV 125
                K+  KK+LD  N ++ +     K   +V   +V  + GT      +VIKR V
Sbjct: 70  WRSSTKKIFKKSLD-DNLNETLKPGSSKMKKKV--REVEKNEGT--DDSFEVIKREV 121

BLAST of Carg20412 vs. TAIR10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related)

HSP 1 Score: 75.5 bits (184), Expect = 1.7e-13
Identity = 44/66 (66.67%), Postives = 47/66 (71.21%), Query Frame = 0

Query: 718 KGISK-NSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEK 777
           +G+ K N     EKVVLRH+ VE KK  Q LFNNVI ET  KL   RKSKVKALVGAFE 
Sbjct: 473 EGVGKINGEGKREKVVLRHRKVEVKKKLQTLFNNVIEETVNKLEEVRKSKVKALVGAFET 532

Query: 778 VISLQD 783
           VISLQD
Sbjct: 533 VISLQD 538

BLAST of Carg20412 vs. TAIR10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related)

HSP 1 Score: 72.8 bits (177), Expect = 1.1e-12
Identity = 84/277 (30.32%), Postives = 126/277 (45.49%), Query Frame = 0

Query: 510 NGTKVVEK-SIITTDPNSVDTVVNLPAIKNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXX 569
           +G+ VV+K   + TD +S  T V        +SKV +    +N  +    +         
Sbjct: 212 SGSAVVKKVPALRTDKSSTSTGVG-------SSKVCAPKNLKNVEKAKTTQTISGEDVKE 271

Query: 570 XXLHVIXXXXXXXXLVESDQNDKHGLKGYQKSPTSLANGPSLSPLREDSGGTKYTKYEAN 629
             + V+          +   ++K  +K   KS ++     S SP ++  G     K    
Sbjct: 272 KTVCVVESSVKGVKSEKQPSSEKKTMKSGNKSLSTTPKRGS-SPTKQIPG-----KISTG 331

Query: 630 ATVSGSKKHGGIKKEDDPNGVKKGKSPRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPT 689
            T    KK  G     + N  K  K  R  +T  K S +  ++F+  KV+D   +   P 
Sbjct: 332 LT---KKKETGSADVVEAN-PKPEKKVRPKKTGVKVSLAQQMTFKKGKVLDPKPEDSSPR 391

Query: 690 RLKFMEGKSLGDNQKSKDGRRTSLKK---GIVKGISKNSTPPSEKVVLRHQDVEGKKDTQ 749
            +KF + + + + +   +G++ +LK    G+            EKVVLRH+ VEGKK   
Sbjct: 392 WIKFKK-RVVQELKTQSEGKKKNLKDRRLGVETKTDSCEGSKREKVVLRHRKVEGKKKMI 451

Query: 750 VLFNNVIAETARKLVRTRKSKVKALVGAFEKVISLQD 783
            LFNNVI ET  KL + RK KVKAL+GAFE VISLQD
Sbjct: 452 TLFNNVIEETVNKLTKVRKHKVKALIGAFETVISLQD 470

BLAST of Carg20412 vs. TrEMBL
Match: tr|A0A251Q5J0|A0A251Q5J0_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G258800 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.1e-23
Identity = 101/255 (39.61%), Postives = 128/255 (50.20%), Query Frame = 0

Query: 545 SQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKGYQKSPTS- 604
           S +K+ NK R+A+ K           L+VI        L ES QN    ++    S +S 
Sbjct: 309 SPLKNHNKIRKAKPKQLNNDEVEEKTLYVIKIETENKPL-ESGQNKNCEVEPSPPSSSSS 368

Query: 605 -----LANGPSLSPLREDSGGTKYTKYEA------NATVSGSKKHGGIKKEDDPNGVKKG 664
                L N  S SP   +   ++YT  E         +    +++    KE D     KG
Sbjct: 369 PQSLCLPNPVSFSPHEGEDHESEYTMSETEDYSXXEDSEVDYRENAETLKEGD-----KG 428

Query: 665 KSPRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSK-DGRRTS 724
           K  +      ++     L FR  KV+D   +   P R        LGDNQ  K D +R S
Sbjct: 429 KPRKSGMVCSEEKDGQPLKFRRGKVVDGKFENDSPRRXXXXXXXXLGDNQNVKADAQRRS 488

Query: 725 LKKGIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALV 784
            KK    G +  + P +EKVVLRHQDVEGKKD + LFNNVI ETA KLV TRKSKVKALV
Sbjct: 489 FKKRGANGDTLATEPGAEKVVLRHQDVEGKKDEKGLFNNVIEETASKLVETRKSKVKALV 548

Query: 785 GAFEKVISLQDKKPS 787
           GAFE VISLQ++KPS
Sbjct: 549 GAFETVISLQERKPS 557

BLAST of Carg20412 vs. TrEMBL
Match: tr|K7MW65|K7MW65_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100794073 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 6.2e-23
Identity = 96/263 (36.50%), Postives = 136/263 (51.71%), Query Frame = 0

Query: 530 VVNLPAIKNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQN 589
           V ++ + K+K+ K+ S +K+Q   R+ + +           LHVI        L +SDQN
Sbjct: 328 VASINSRKHKSLKIASHLKNQCTARKDENEEHNNNVLEEKTLHVIKMESENKTL-QSDQN 387

Query: 590 DKHGLKGY---QKSPTSLANGPSLSPLREDSGGTKYTKYE-ANATVSGSKKHGGIKKEDD 649
             +  + Y     SP S  +  S S  +ED   ++YT  E    + SG+ +   ++ E+ 
Sbjct: 388 ASYDDESYLPQLSSPKSSVSSISKSLSQEDQEESEYTTSEFEQDSFSGNHEIECMENEET 447

Query: 650 PNGVKKGKSPR--MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQK 709
               KKGK  +  ++ ++GK +    L FR  KV++  ++   P R        LG+   
Sbjct: 448 LGVEKKGKPKKDGVVYSRGKGNQMIKLKFRRGKVVENQTEISSPRRXXXXXXXVLGEKAN 507

Query: 710 SKDGRRTSLKKGIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTR 769
            K   R S +       S  +T   EKVVLRHQD++ KKD Q L NNVI ETA KLV  R
Sbjct: 508 GKVNVRKSFEGNEACDDSNGATTGPEKVVLRHQDMQDKKDAQGLLNNVIEETASKLVEAR 567

Query: 770 KSKVKALVGAFEKVISLQDKKPS 787
           KSKVKALVGAFE VISL +KKPS
Sbjct: 568 KSKVKALVGAFETVISLHEKKPS 589


HSP 2 Score: 60.5 bits (145), Expect = 2.0e-05
Identity = 46/140 (32.86%), Postives = 67/140 (47.86%), Query Frame = 0

Query: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60
           M + S +L ++PE   S G  +RR S GKA S +  E+      R+S GSCHD CKYG  
Sbjct: 2   MANESGELPVTPEVTESPGVEIRRHSDGKASSGTSVEKFVPNYLRASTGSCHDFCKYGKK 61

Query: 61  HSLETKARVPLLKRAMKKAL--DAQNSDQAVVVP----------EKKHSVRVTKSKVSPS 120
           H+ ++K R  +  RA +K L   ++ S    ++P           K   +   K K+S  
Sbjct: 62  HAFDSKERRSIPNRATRKLLHHSSEGSVGGTMMPVARLSASVDSTKSTKISTVKLKLSVD 121

Query: 121 PGTCISGGTDVIKRVVPISS 129
             T IS  +D  K+  P  S
Sbjct: 122 SKTWISDISDTYKQERPTKS 141

BLAST of Carg20412 vs. TrEMBL
Match: tr|A0A0B2SWB0|A0A0B2SWB0_GLYSO (Uncharacterized protein OS=Glycine soja OX=3848 GN=glysoja_030569 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 6.2e-23
Identity = 96/263 (36.50%), Postives = 136/263 (51.71%), Query Frame = 0

Query: 530 VVNLPAIKNKNSKVVSQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQN 589
           V ++ + K+K+ K+ S +K+Q   R+ + +           LHVI        L +SDQN
Sbjct: 327 VASINSRKHKSLKIASHLKNQCTARKDENEEHNNNVLEEKTLHVIKMESENKTL-QSDQN 386

Query: 590 DKHGLKGY---QKSPTSLANGPSLSPLREDSGGTKYTKYE-ANATVSGSKKHGGIKKEDD 649
             +  + Y     SP S  +  S S  +ED   ++YT  E    + SG+ +   ++ E+ 
Sbjct: 387 ASYDDESYLPQLSSPKSSVSSISKSLSQEDQEESEYTTSEFEQDSFSGNHEIECMENEET 446

Query: 650 PNGVKKGKSPR--MLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQK 709
               KKGK  +  ++ ++GK +    L FR  KV++  ++   P R        LG+   
Sbjct: 447 LGVEKKGKPKKDGVVYSRGKGNQMIKLKFRRGKVVENQTEISSPRRXXXXXXXVLGEKAN 506

Query: 710 SKDGRRTSLKKGIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTR 769
            K   R S +       S  +T   EKVVLRHQD++ KKD Q L NNVI ETA KLV  R
Sbjct: 507 GKVNVRKSFEGNEACDDSNGATTGPEKVVLRHQDMQDKKDAQGLLNNVIEETASKLVEAR 566

Query: 770 KSKVKALVGAFEKVISLQDKKPS 787
           KSKVKALVGAFE VISL +KKPS
Sbjct: 567 KSKVKALVGAFETVISLHEKKPS 588

BLAST of Carg20412 vs. TrEMBL
Match: tr|M5WS70|M5WS70_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa007111mg PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 8.2e-23
Identity = 99/249 (39.76%), Postives = 124/249 (49.80%), Query Frame = 0

Query: 545 SQVKSQNKTRRAQAKXXXXXXXXXXXLHVIXXXXXXXXLVESDQNDKHGLKGYQKSPTSL 604
           S +K+ NK R+A+ K           L+VI        L ES QN              L
Sbjct: 141 SPLKNHNKIRKAKPKQLNNDEVEEKTLYVIKIETENKPL-ESGQNK-------NCESLCL 200

Query: 605 ANGPSLSPLREDSGGTKYTKYE------ANATVSGSKKHGGIKKEDDPNGVKKGKSPRML 664
            N  S SP   +   ++YT  E      +  +    +++    KE D     KGK  +  
Sbjct: 201 PNPVSFSPHEGEDHESEYTMSETEDYSFSEDSEVDYRENAETLKEGD-----KGKPRKSG 260

Query: 665 QTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLGDNQKSK-DGRRTSLKKGIV 724
               ++     L FR  KV+D   +   P R        LGDNQ  K D +R S KK   
Sbjct: 261 MVCSEEKDGQPLKFRRGKVVDGKFENDSPRRXXXXXXXXLGDNQNVKADAQRRSFKKRGA 320

Query: 725 KGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETARKLVRTRKSKVKALVGAFEKV 784
            G +  + P +EKVVLRHQDVEGKKD + LFNNVI ETA KLV TRKSKVKALVGAFE V
Sbjct: 321 NGDTLATEPGAEKVVLRHQDVEGKKDEKGLFNNVIEETASKLVETRKSKVKALVGAFETV 376

Query: 785 ISLQDKKPS 787
           ISLQ++KPS
Sbjct: 381 ISLQERKPS 376

BLAST of Carg20412 vs. TrEMBL
Match: tr|A0A2K3LHA9|A0A2K3LHA9_TRIPR (Serine-rich adhesin for platelets-like protein (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g033899 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-21
Identity = 82/209 (39.23%), Postives = 122/209 (58.37%), Query Frame = 0

Query: 584 VESDQNDKHGLKGY-QKSPTSLANGPSLS-PLREDSGGTKYT--KYEANATVSGSKKHGG 643
           ++SDQ   +  + Y  +  T +++  S++  L ++   ++YT  ++E ++    + +   
Sbjct: 463 LQSDQTASYDDESYLPQLSTPMSSSTSVTQSLSDEDEESEYTTSEFEVDSLSGINCEIEC 522

Query: 644 IKKEDDPNGVKKGKSPRMLQTKGKDSSSFSLSFRNKKVIDLDSKRHGPTRLKFMEGKSLG 703
           ++ E+     KK K  ++ + + KD     L FR  KV+D  ++++ P RL F   K+L 
Sbjct: 523 MENEEALKVEKKSKPRKVKEVEDKDCEMIKLKFRRGKVVDKQTEKNTPRRLTFRRAKTLA 582

Query: 704 D--NQKSKDGRRTSLKKGIVKGISKNSTPPSEKVVLRHQDVEGKKDTQVLFNNVIAETAR 763
           +  N  +   R++  K+      S ++    EKVVLRHQDVE KKD Q LFNNVI ETA 
Sbjct: 583 EKANVNNNSERKSFKKRDEACSESNDAVNGQEKVVLRHQDVEDKKDAQGLFNNVIEETAS 642

Query: 764 KLVRTRKSKVKALVGAFEKVISLQDKKPS 787
           KLV  RKSKVKALVGAFE VISLQDKKPS
Sbjct: 643 KLVEARKSKVKALVGAFETVISLQDKKPS 671


HSP 2 Score: 52.8 bits (125), Expect = 4.2e-03
Identity = 37/108 (34.26%), Postives = 61/108 (56.48%), Query Frame = 0

Query: 1   MVDGSMDLSMSPETHRSDGDRLRRLSMGKAISLSIDEQNNFRDRRSSIGSCHDICKYGHN 60
           M   S++  ++P+  +S   ++RR S G A S + DE+      R+S GSCHD+CKYG  
Sbjct: 19  MASESVESLVTPDVTKSPEVKVRRNSAGNARSGNGDEKVVPNYLRASTGSCHDLCKYGGE 78

Query: 61  HSLETKARVPLLKRAMKKALDAQNSDQAV--VVPEKKHSVRVTKSKVS 107
           H+  +K  +P   RA +K L  Q+S+ ++  V+ + K  V    +K+S
Sbjct: 79  HAFVSKRSIP--NRATRKKLH-QSSEASIGQVIAKSKPPVDSKPTKMS 123

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023523723.11.1e-23191.33uncharacterized protein LOC111787871 [Cucurbita pepo subsp. pepo] >XP_023523724.... [more]
XP_022949115.12.3e-23194.97muscle M-line assembly protein unc-89-like isoform X2 [Cucurbita moschata][more]
XP_022949113.14.0e-23197.12muscle M-line assembly protein unc-89-like isoform X1 [Cucurbita moschata] >XP_0... [more]
XP_022997888.11.3e-21888.07muscle M-line assembly protein unc-89-like isoform X2 [Cucurbita maxima][more]
XP_022997890.11.3e-21888.07muscle M-line assembly protein unc-89-like isoform X4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G15430.19.4e-1743.17Plant calmodulin-binding protein-related[more]
AT5G39380.17.5e-1479.63Plant calmodulin-binding protein-related[more]
AT5G07820.11.7e-1366.67Plant calmodulin-binding protein-related[more]
AT5G61260.11.1e-1230.32Plant calmodulin-binding protein-related[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
tr|A0A251Q5J0|A0A251Q5J0_PRUPE2.1e-2339.61Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G258800 PE=4 SV=1[more]
tr|K7MW65|K7MW65_SOYBN6.2e-2336.50Uncharacterized protein OS=Glycine max OX=3847 GN=100794073 PE=4 SV=1[more]
tr|A0A0B2SWB0|A0A0B2SWB0_GLYSO6.2e-2336.50Uncharacterized protein OS=Glycine soja OX=3848 GN=glysoja_030569 PE=4 SV=1[more]
tr|M5WS70|M5WS70_PRUPE8.2e-2339.76Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa007111mg PE=4 SV=1[more]
tr|A0A2K3LHA9|A0A2K3LHA9_TRIPR1.2e-2139.23Serine-rich adhesin for platelets-like protein (Fragment) OS=Trifolium pratense ... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005516calmodulin binding
Vocabulary: INTERPRO
TermDefinition
IPR012417CaM-bd_dom_pln
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005516 calmodulin binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20412-RACarg20412-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 666..779
e-value: 1.8E-25
score: 100.6
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 670..779
e-value: 5.0E-30
score: 104.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 688..732
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 688..713
NoneNo IPR availablePANTHERPTHR33349:SF1CALMODULIN-BINDING PROTEIN-LIKE PROTEINcoord: 1..368
coord: 331..787
NoneNo IPR availablePANTHERPTHR33349FAMILY NOT NAMEDcoord: 1..368
coord: 331..787