Carg20106 (gene) Silver-seed gourd

Overview
NameCarg20106
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionIsoleucine-tRNA ligase
LocationCucurbita_argyrosperma_scaffold_168 : 172024 .. 175977 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCATTAGTTTCCTCTCTCATACTTCCTATCGCCCCGAGGATGAAGAGGCTCTGCAAATGAATGGAGTCAATATGCCGCTGTATGCGTCCTTTGCCGCCGGCTCTTCCGCATTGAAGTGCAAAGGAGCCACCAAATCTCTCATTCAAACGTCTTCTTATTCAGTACAATTTCTCTCTTCTCCTCTTTATCTCTCTGTTTACGCCAGTCTTGTTGAATTTTGAAGCAGTCTACTTCGATGATTCAAGAACGATTTCTTCTTTGTTTACGTTTCTTTGTGTTATTCTGTCGTTTTTTTCTGATAAAAGAAGGTTTTATGGAGTTAATTTGATTACTTCATATCCTTCTTGTTGAGTTTAGAGAGTGTAGTGGTTAATTTGCTTATGTTTGTTCAGTGCGTTTGTATTGGAAATTGTTTGGAGTGCGTTTTTCTGTGTTTGAATTATTGCTCCTTCTATTGGGTTATCTTCTAATACCACGTTTCAATGTTCATAACGATTCAATTTTGATACGCTATTGACGTTTGATTCTGCAGACTTGTGCTTGTGTCTTGGTAAATTCAATCTCGTGAAAGAACATTGGGGTGTATTCTTTATCTTATTATTTTTATAAATTGCTTAACCTAAGAGGTAAAGTGTGAGATCCCACATTGGTTGGGGAGGAGAAAGAAACATTCTTTACAAGGGTGCGGAAACCTCTCCCTAGTAGACACGTTTTAAAAATCTTGAGGAGAAGTCTGAAAGGGAAAGCCCAAAGAGGATAATATTTGCTAGCGGTGGCTTGGGCTGTTACAAATGGTATCAGAGCCAGACATCGGGTGGTGTGCCAACGAGGAGACTGAACCTTGAAGGGGTGTACATGAGGCGGTGTGCTAGCAAGGACGCTGGGCCTCAAAGGGGGGTCCCACTTCGATTGGGGAAAGGAATGAGTGCCAACAATGACGTTGGGTCTCGAAAGGGGGTGTACATGAGGCGGTGTGCCAGCAAGGACGCTAGCCCCAAAGGGGGGTCCCACATCGATTAGGGAAAGGAACGAGTGTCAGCGATGACGCTGGACCCCGAAGGGGGTGGATTGTGAGACCCCACATCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTAAAAACCTTGAGGGGAAGCCCGAAAGGGAGCGGTGAGCTTGGACTGTTACATAGAGCAAAAATACCACGTGATAATGATGTTTTTCTTTTTGGTCGGAAAGAAAAAAAATCAAAATTTATTTTAGGAGGTACTTCTAGCTGTTCAGTTATGGAAAATGGTGTTTACCCTTAACGATCAAATCATCTGTGACCGTTATTCTTGAATGTGAATTGCTGGTGCTCGATGTCAATTTACTCGTGTTTCATATTTCCAGGTATTATCACAAAGAATTTCTGCACCACTTGGGAGAAGTTATATTCCTTCATTTCGTTTTAGAGGAAGCTCTTCGGCAAAAGTATTTATGTTCTTGAGCGTCACACGTTACTCTACTTATTTAAATGATGAATTCCTACCTTCCTCAAAGAGAAGGGCTAGAGGGCCCGTTATGGCAGCAAAGAAAGCATCTAAAGGTGCATAGTCTCGGAAATTATGTATTACATTTCCCTTCAAATCTATGCTTTTCATTTTTATGAATTACATGACTTCTTGAATGAGTTTTTGCATTTTTTCAATGAAGTTTCTGATTTCTTGGATTGCTTGAGTTTGTTTAGAGACAAATGAAGGAGATGGAAAATACAAGCATACTGTTGATCTGCCAAAGACTACATTTGGCATGAGAGCAAATTCTACGGTTAGGGAGCCAGAAATTCAGAAAATATGGGATGACCATCAAGTGTTCAATCGAGTTGTTGAAAGAAATACTGGAGTACGTTCTTCGGTCTTCTTGGGATATCGTCCTTCTTTTGTCCACCATTCAAAGTAACTTATTTGCCGACATCTGGTCTCAGGAAAACTTCATTCTTCACGATGGTCCTCCATATGCCAATGGTGATCTTCACATGGGCCATGCTTTGAATAAGATTTTGAAGGATATAATTAACCGTTACAAGGTTAGTTTCTTATTCTTAGCCACACTTCATTAAATCAGACAACATGCCAGAAAAAAAAAAAAAAAAAAAGCTGGAAAATGTTAATAATGTATGTCTGTATCCCTAGTAATTATTTTCAACACCTCATACTGAAGTTCTGTTGAGATTCAGTTTCGGTTACTGTTGCCTTGGAGTTTATATACAAACGGTGTCTAATTTTAACTCGTGATCCCTCCGCCATGTAATAGCTACTTAGTTTTTGGAAGAACAAACTCCTTGCTTGACATTTTAAAAGTTTCTAACTAGTATTTATTTTTCCTTTTATTTTCTTCTCCTAACAGCTTCTTCAAAACTATAAAGTTCGTTTTGTGCCTGGCTGGGACTGCCATGGGCTACCAATTGAGTTAAAAGGCAAGTATCTTTTGCAGTAGTTAACCTCCGTGATAAGCTTTTACTGAGGGGTAACGTCTTAGGTCTCTAGGGACAAAGGTAAGGAAACTTAACTGTAATCTTGAGCTATTCAGTTCTGCAGTCATTGGATAAAGAAGCCAGGAAAGATCTTACCCCAATTAAGTTGCGAGATAAGGCTGCCAAGTTTGCTAAAGCTACGGTGAAAGCTCAGATGGCATCATTTAAGGCAATTTCCTCATCACCTCAACTTTATTTATTTCAATGCTTAATATTTCTTTCGTCATGTTAAAGTGATCAATCTTATGTGATAATTTTGGTTCATGTCAACATTATGTCTATGCGAGCATGTTCATCTTTAATGCTGTCTGAGTACTGGGTTTAGTGTGTCGTGGGAGTTACTCTTCATAGAAAATTTCATGACTCTCTTCTTTTAAATTTCTCAAAGTATGCTGTTCAATTTTTGCAGCGCTATGGAGTATGGGCAGATTGGAACAATCCGTACCTAACTCTCAGTCCAGAATATGAAGCTGCCCAGGTAACTATTGCATGCATTCTAATGTTCTACTTTCTATGTGCTTCCTTTAAGCACTTTGTTGCAGAATTGAATTCAATAGTTTTGTGTTTGTATTGTTTTTATGTTAGATTGAGGTGTTTGGTCAAATGGCCATTCAAGGATATATTTTTAGAGGGAGAAAGCCAGTTCACTGGAGTCCCTCATCACGAACTGCTCTTGCCGAGGCTGAATTGGAGGTAAATCAACTGTTGAAGCATGAGAGCATGAACAAATTAGATAATCTTATTACAGTTGTGGGACGAAGCTTTAGGGTTCTTGATAAACTTTGCCTCTAATCTATATCTCATTCGGCTTCCTATCATTTGCTCAAATTTGACATTATCTTTTTTTTTTTCTTTTCCCTGCACTACCTGTTTGATAACTGCATTCAGTACCCTGAGGGGCATACTTCAAGAAGCATATATGCTATTTTTAGATTGGTCAAGGAAGGTCCATCTTCAGGCGGCATGCTACAAGAATTTCTTCCAGACTTGTGCTTGGCCATATGGACTACAACTCCTTGGACTATTCCAGCCAATGCTGGTGAGTTCTTTTTTGTGTTACCGTGGGAGCAATGTGGTAGTAGATTTATTCCTGTATGTTTCCCAGTTATATTATTATTATTACAGTGGGAGCAAGTGTCCGGGCCGGCTTGCGTGCACCTTGGCTACTCTCATGGGACAATTGCATGATTCTACTACACTTGAGCAGTCTATCTTTACGTTGTATACATTCTAAACTTTTTCCCTCACTGTTCTGTGATTAGCGGTTGCTGTCAATGCCAAGCTTCACTATGCCATAGTTGAACTGCTGGCACCGTCAGAAGATGTCTCTTTTTTGTCTGGAAATAAAAAGAAAAGACCTGGGAATATTCTGAATGAGGAAAATAGGCCTTTCCTTGTTGTAGCATCAGACCTGGTGCCAACTTTGGAAGCAAAATGGGGTGTGAAGCTGTGA

mRNA sequence

CGCATTAGTTTCCTCTCTCATACTTCCTATCGCCCCGAGGATGAAGAGGCTCTGCAAATGAATGGAGTCAATATGCCGCTGTATGCGTCCTTTGCCGCCGGCTCTTCCGCATTGAAGTGCAAAGGAGCCACCAAATCTCTCATTCAAACGTCTTCTTATTCAGTATTATCACAAAGAATTTCTGCACCACTTGGGAGAAGTTATATTCCTTCATTTCGTTTTAGAGGAAGCTCTTCGGCAAAAGTATTTATGTTCTTGAGCGTCACACGTTACTCTACTTATTTAAATGATGAATTCCTACCTTCCTCAAAGAGAAGGGCTAGAGGGCCCGTTATGGCAGCAAAGAAAGCATCTAAAGAGACAAATGAAGGAGATGGAAAATACAAGCATACTGTTGATCTGCCAAAGACTACATTTGGCATGAGAGCAAATTCTACGGTTAGGGAGCCAGAAATTCAGAAAATATGGGATGACCATCAAGTGTTCAATCGAGTTGTTGAAAGAAATACTGGAGAAAACTTCATTCTTCACGATGGTCCTCCATATGCCAATGGTGATCTTCACATGGGCCATGCTTTGAATAAGATTTTGAAGGATATAATTAACCGTTACAAGCTTCTTCAAAACTATAAAGTTCGTTTTGTGCCTGGCTGGGACTGCCATGGGCTACCAATTGAGTTAAAAGGCAAGAAAGATCTTACCCCAATTAAGTTGCGAGATAAGGCTGCCAAGTTTGCTAAAGCTACGCGCTATGGAGTATGGGCAGATTGGAACAATCCGTACCTAACTCTCAGTCCAGAATATGAAGCTGCCCAGGTAACTATTGCATGCATTCTAATGTTCTACTTTCTATGTGCTTCCTTTAAGCACTTTGTTGCAGAATTGAATTCAATAGTTTTGTGTTTGTATTGTTTTTATGTTAGATTGAGGTGTTTGGTCAAATGGCCATTCAAGGATATATTTTTAGAGGGAGAAAGCCAGTTCACTGGAGTCCCTCATCACGAACTGCTCTTGCCGAGGCTGAATTGGAGTACCCTGAGGGGCATACTTCAAGAAGCATATATGCTATTTTTAGATTGGTCAAGGAAGGTCCATCTTCAGGCGGCATGCTACAAGAATTTCTTCCAGACTTGTGCTTGGCCATATGGACTACAACTCCTTGGACTATTCCAGCCAATGCTGGTGAGTTCTTTTTTGTGTTACCCGGTTGCTGTCAATGCCAAGCTTCACTATGCCATAGTTGAACTGCTGGCACCGTCAGAAGATGTCTCTTTTTTGTCTGGAAATAAAAAGAAAAGACCTGGGAATATTCTGAATGAGGAAAATAGGCCTTTCCTTGTTGTAGCATCAGACCTGGTGCCAACTTTGGAAGCAAAATGGGGTGTGAAGCTGTGA

Coding sequence (CDS)

ATGAATGGAGTCAATATGCCGCTGTATGCGTCCTTTGCCGCCGGCTCTTCCGCATTGAAGTGCAAAGGAGCCACCAAATCTCTCATTCAAACGTCTTCTTATTCAGTATTATCACAAAGAATTTCTGCACCACTTGGGAGAAGTTATATTCCTTCATTTCGTTTTAGAGGAAGCTCTTCGGCAAAAGTATTTATGTTCTTGAGCGTCACACGTTACTCTACTTATTTAAATGATGAATTCCTACCTTCCTCAAAGAGAAGGGCTAGAGGGCCCGTTATGGCAGCAAAGAAAGCATCTAAAGAGACAAATGAAGGAGATGGAAAATACAAGCATACTGTTGATCTGCCAAAGACTACATTTGGCATGAGAGCAAATTCTACGGTTAGGGAGCCAGAAATTCAGAAAATATGGGATGACCATCAAGTGTTCAATCGAGTTGTTGAAAGAAATACTGGAGAAAACTTCATTCTTCACGATGGTCCTCCATATGCCAATGGTGATCTTCACATGGGCCATGCTTTGAATAAGATTTTGAAGGATATAATTAACCGTTACAAGCTTCTTCAAAACTATAAAGTTCGTTTTGTGCCTGGCTGGGACTGCCATGGGCTACCAATTGAGTTAAAAGGCAAGAAAGATCTTACCCCAATTAAGTTGCGAGATAAGGCTGCCAAGTTTGCTAAAGCTACGCGCTATGGAGTATGGGCAGATTGGAACAATCCGTACCTAACTCTCAGTCCAGAATATGAAGCTGCCCAGGTAACTATTGCATGCATTCTAATGTTCTACTTTCTATGTGCTTCCTTTAAGCACTTTGTTGCAGAATTGAATTCAATAGTTTTGTGTTTGTATTGTTTTTATGTTAGATTGAGGTGTTTGGTCAAATGGCCATTCAAGGATATATTTTTAGAGGGAGAAAGCCAGTTCACTGGAGTCCCTCATCACGAACTGCTCTTGCCGAGGCTGAATTGGAGTACCCTGAGGGGCATACTTCAAGAAGCATATATGCTATTTTTAGATTGGTCAAGGAAGGTCCATCTTCAGGCGGCATGCTACAAGAATTTCTTCCAGACTTGTGCTTGGCCATATGGACTACAACTCCTTGGACTATTCCAGCCAATGCTGGTGAGTTCTTTTTTGTGTTACCCGGTTGCTGTCAATGCCAAGCTTCACTATGCCATAGTTGAACTGCTGGCACCGTCAGAAGATGTCTCTTTTTTGTCTGGAAATAAAAAGAAAAGACCTGGGAATATTCTGAATGAGGAAAATAGGCCTTTCCTTGTTGTAGCATCAGACCTGGTGCCAACTTTGGAAGCAAAATGGGGTGTGAAGCTGTGA

Protein sequence

MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSSAKVFMFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIELKGKKDLTPIKLRDKAAKFAKATRYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFYVRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSRKVHLQAACYKNFFQTCAWPYGLQLLGLFQPMLVSSFLCYPVAVNAKLHYAIVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL
Homology
[Close]
BLAST of Carg20106 vs. NCBI nr
Match: XP_022956209.1 (isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 589.3 bits (1518), Expect = 1.1e-164
Identity = 330/471 (70.06%), Postives = 343/471 (72.82%), Query Frame = 0 

Query: 1   MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSS 60
           MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISA LGRSYIPSFRFRGSSS
Sbjct: 1   MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISASLGRSYIPSFRFRGSSS 60

Query: 61  AKVFMFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTF 120
           AKVF FLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTF
Sbjct: 61  AKVFTFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTF 120

Query: 121 GMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKD 180
           GMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKD
Sbjct: 121 GMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKD 180

Query: 181 IINRYKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT-- 240
           IINRYKLLQNYKVRFVPGWDCHGLPIELK         +KDLTPIKLRDKAAKFAKAT  
Sbjct: 181 IINRYKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVK 240

Query: 241 -------RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCL 300
                  RYGVWADWNNPYLTLSPEYEAAQ+ +                      + +  
Sbjct: 241 AQMASFKRYGVWADWNNPYLTLSPEYEAAQIEV-------------------FGQMAIQG 300

Query: 301 YCFYVRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSR 360
           Y F  R R  V W          S  T +   EL  P                       
Sbjct: 301 YIF--RGRKPVHW--------SPSSRTALAEAELEYP----------------------- 360

Query: 361 KVHLQAACYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYAI 420
           + H   + Y  F      P    +L  F P + ++ +   P        VAVNAKLHYAI
Sbjct: 361 EGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAI 419

Query: 421 VELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           VELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL
Sbjct: 421 VELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 419

[Close]
BLAST of Carg20106 vs. NCBI nr
Match: XP_022979679.1 (isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 584.7 bits (1506), Expect = 2.6e-163
Identity = 326/471 (69.21%), Postives = 341/471 (72.40%), Query Frame = 0 

Query: 1   MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSS 60
           MNGVNMPLYASFAAGSSALKCKG TKSL+QTSSYSVLSQRISA LGRSYIPSFRFRGSSS
Sbjct: 1   MNGVNMPLYASFAAGSSALKCKGVTKSLVQTSSYSVLSQRISASLGRSYIPSFRFRGSSS 60

Query: 61  AKVFMFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTF 120
           AKVF FLSVT YSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTF
Sbjct: 61  AKVFTFLSVTHYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTF 120

Query: 121 GMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKD 180
           GMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHA+NKILKD
Sbjct: 121 GMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHAMNKILKD 180

Query: 181 IINRYKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT-- 240
           IINRYKLLQNYKVRFVPGWDCHGLPIELK         +KDLTPIKLRDKAAKFAKAT  
Sbjct: 181 IINRYKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVK 240

Query: 241 -------RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCL 300
                  RYGVWADWNNPYLTLSPEYEAAQ+ +                      + +  
Sbjct: 241 AQMASFKRYGVWADWNNPYLTLSPEYEAAQIEV-------------------FGQMAIQG 300

Query: 301 YCFYVRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSR 360
           Y F  R R  V W          S  T +   EL  P                       
Sbjct: 301 YIF--RGRKPVHW--------SPSSRTALAEAELEYP----------------------- 360

Query: 361 KVHLQAACYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYAI 420
           + H   + Y  F      P    +L  F P + ++ +   P        VAVNAKLHYAI
Sbjct: 361 EGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAI 419

Query: 421 VELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           VELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL
Sbjct: 421 VELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 419

[Close]
BLAST of Carg20106 vs. NCBI nr
Match: XP_023527875.1 (isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.7 bits (1506), Expect = 2.6e-163
Identity = 330/472 (69.92%), Postives = 343/472 (72.67%), Query Frame = 0 

Query: 1   MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLG-RSYIPSFRFRGSS 60
           MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISA LG RSYIPSFRFRGSS
Sbjct: 1   MNGVNMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISASLGRRSYIPSFRFRGSS 60

Query: 61  SAKVFMFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTT 120
           SAKVF FLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTT
Sbjct: 61  SAKVFTFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTT 120

Query: 121 FGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILK 180
           FGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILK
Sbjct: 121 FGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILK 180

Query: 181 DIINRYKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT- 240
           DIINRYKLLQNYKVRFVPGWDCHGLPIELK         +KDLTPIKLRDKAAKFAKAT 
Sbjct: 181 DIINRYKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATV 240

Query: 241 --------RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLC 300
                   RYGVWADWNNPYLTLSPEYEAAQ+ +                      + + 
Sbjct: 241 KTQMASFKRYGVWADWNNPYLTLSPEYEAAQIEV-------------------FGQMAIQ 300

Query: 301 LYCFYVRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWS 360
            Y F  R R  V W          S  T +   EL  P                      
Sbjct: 301 GYIF--RGRKPVHW--------SPSSRTALAEAELEYP---------------------- 360

Query: 361 RKVHLQAACYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYA 420
            + H   + Y  F      P    +L  F P + ++ +   P        VAVNAKLHYA
Sbjct: 361 -EGHTSRSIYAIFRLVKEGPSSGGMLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYA 420

Query: 421 IVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           IVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL
Sbjct: 421 IVELLAPSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 420

[Close]
BLAST of Carg20106 vs. NCBI nr
Match: XP_008438885.1 (PREDICTED: isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 536.6 bits (1381), Expect = 8.2e-149
Identity = 301/467 (64.45%), Postives = 324/467 (69.38%), Query Frame = 0 

Query: 5   NMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSSAKVF 64
           NMPLYASFAAGSSA KCK ATK  IQT SY+VLSQRIS+ L RSYI SFRFRGSSSAKVF
Sbjct: 4   NMPLYASFAAGSSAFKCKEATKPFIQTPSYTVLSQRISSTLRRSYISSFRFRGSSSAKVF 63

Query: 65  MFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRA 124
            FL+VTRYSTYLNDEF PSSKRR+RGP+MAAKKAS+ETNEGDGKYKHTVDLPKTTFGMRA
Sbjct: 64  TFLTVTRYSTYLNDEFPPSSKRRSRGPIMAAKKASQETNEGDGKYKHTVDLPKTTFGMRA 123

Query: 125 NSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 184
           NS VREPEIQKIWDDHQVFNR VERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR
Sbjct: 124 NSLVREPEIQKIWDDHQVFNRAVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 183

Query: 185 YKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT------ 244
           YKLLQNYKVRFVPGWDCHGLPIELK         +KDLTPIKLRDKAAKFAKAT      
Sbjct: 184 YKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMA 243

Query: 245 ---RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFY 304
              RYGVWADWNNPYLTLSPEYEAAQ+ +                      + +  Y F 
Sbjct: 244 SFKRYGVWADWNNPYLTLSPEYEAAQIEV-------------------FGQMAIQGYIF- 303

Query: 305 VRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSRKVHL 364
            R R  V W          S  T +   EL  P                       + H 
Sbjct: 304 -RGRKPVHW--------SPSSRTALAEAELEYP-----------------------EGHT 363

Query: 365 QAACYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYAIVELL 424
             + Y  F      P    +L  F P + ++ +   P        VAVNAKLHYAI+ELL
Sbjct: 364 SRSIYAIFRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAIIELL 418

Query: 425 APSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           APSED S L+GNKKK+PGNIL E  RPFLVVASDLVPTLEAKWGVK+
Sbjct: 424 APSEDASVLTGNKKKKPGNILKEGKRPFLVVASDLVPTLEAKWGVKI 418

[Close]
BLAST of Carg20106 vs. NCBI nr
Match: XP_011651056.1 (PREDICTED: LOW QUALITY PROTEIN: probable isoleucine--tRNA ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 536.6 bits (1381), Expect = 8.2e-149
Identity = 304/467 (65.10%), Postives = 324/467 (69.38%), Query Frame = 0 

Query: 5   NMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSSAKVF 64
           NMPLYASFAAGSSA KCK ATK  IQT SY+VLSQRIS+ L RSYI SFRFRGSSSAKVF
Sbjct: 4   NMPLYASFAAGSSAFKCKEATKPFIQTPSYTVLSQRISSTLRRSYISSFRFRGSSSAKVF 63

Query: 65  MFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRA 124
            FL+VTRYSTYLNDEF PSSKRR+RGP+MAAKKASKETNEGDGKYKHTVDLPKT FGMRA
Sbjct: 64  TFLTVTRYSTYLNDEFPPSSKRRSRGPIMAAKKASKETNEGDGKYKHTVDLPKTAFGMRA 123

Query: 125 NSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 184
           NS VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR
Sbjct: 124 NSLVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 183

Query: 185 YKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT------ 244
           YKLLQNYKVRFVPGWDCHGLPIELK         +KDLTPIKLRDKAAKFAKAT      
Sbjct: 184 YKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMA 243

Query: 245 ---RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFY 304
              RYGVWAD NNPYLTLSPEYEAAQ+ +                      + +  Y F 
Sbjct: 244 SFKRYGVWADXNNPYLTLSPEYEAAQIEV-------------------FGQMAIQGYIF- 303

Query: 305 VRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSRKVHL 364
            R R  V W          S  T +   EL  P                       + H 
Sbjct: 304 -RGRKPVHW--------SPSSRTALAEAELEYP-----------------------EGHT 363

Query: 365 QAACYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYAIVELL 424
             + Y  F      P    +L  F P + ++ +   P        VAVNAKLHYAI+ELL
Sbjct: 364 SRSIYAIFRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAIIELL 418

Query: 425 APSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           APSEDVS L+GNKKKRPGNIL E  RPFLVVASDLVPTLEAKWGVKL
Sbjct: 424 APSEDVSVLTGNKKKRPGNILKEGKRPFLVVASDLVPTLEAKWGVKL 418

[Close]
BLAST of Carg20106 vs. TAIR10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein)

HSP 1 Score: 374.4 bits (960), Expect = 9.7e-104
Identity = 222/447 (49.66%), Postives = 273/447 (61.07%), Query Frame = 0 

Query: 22  KGATKSLIQTSSYSVLSQRISAPLGRSY-IPSFRFRGSSSAKVFMFLSVTRYSTYLNDEF 81
           + A  +++Q+SSY VLS +  + L R+  + SF  +G SS K F FL V+R+ST  N+EF
Sbjct: 14  EAAAMAMVQSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSVKAFSFLYVSRFSTEPNNEF 73

Query: 82  LPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRANSTVREPEIQKIWDDH 141
             SSKRR+RGPVMAAKKAS+   + DGKYK TVDLPKT FGMRANS  REPE+QK+W+++
Sbjct: 74  GHSSKRRSRGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFGMRANSLTREPELQKLWEEN 133

Query: 142 QVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVRFVPGWD 201
           QVF RV + N G +FILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKV++VPGWD
Sbjct: 134 QVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 193

Query: 202 CHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT---------RYGVWADWNNPYL 261
           CHGLPIELK         +K+LTP+KLR KAAKFAKAT         R+GVWADWNNPYL
Sbjct: 194 CHGLPIELKVLQSLDQEVRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNNPYL 253

Query: 262 TLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFYVRLRCLVKWPFKDIFL 321
           TL PEYEAAQ+ +       F   + K ++               R R  V W       
Sbjct: 254 TLDPEYEAAQIEV-------FGQMALKGYI--------------YRGRKPVHW------- 313

Query: 322 EGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLF-LDWSRKVHLQAACYKNFF----QT 381
              S  T +   EL  P  +      I +  Y +F L    K  L      N +     T
Sbjct: 314 -SPSSRTALAEAELEYPEGH------ISKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTT 373

Query: 382 CAWPYGLQLLGLFQPMLVSSFLCYPVAVNAKLHYAIVELLAPSEDVSFLSGNKKKRPGNI 441
             W                      VAVNAKL Y++VE+ + SED S ++ NKKK PG +
Sbjct: 374 TPWTMPANA---------------AVAVNAKLQYSVVEVQSFSEDESTVTSNKKKIPGKV 410

Query: 442 LNEENRPFLVVASDLVPTLEAKWGVKL 446
           L  + + F++VA+DLVP LEAKWGVKL
Sbjct: 434 LKNQQKLFVIVATDLVPALEAKWGVKL 410

[Close]
BLAST of Carg20106 vs. TAIR10
Match: AT4G10320.1 (tRNA synthetase class I (I, L, M and V) family protein)

HSP 1 Score: 79.3 bits (194), Expect = 6.5e-15
Identity = 44/139 (31.65%), Postives = 64/139 (46.04%), Query Frame = 0 

Query: 129 REPEIQKIWDDHQVFNRVVERNTG-ENFILHDGPPYANGDLHMGHALNKILKDIINRYKL 188
           +E ++   W +   F   ++R      +I +DGPP+A G  H GH L   +KDI+ RY+ 
Sbjct: 15  QEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQT 74

Query: 189 LQNYKVRFVPGWDCHGLPIELKGKKDLTPIKLRDKAAKFA-------------------- 246
           +  + V    GWDCHGLP+E +  + L  IK RD+  K                      
Sbjct: 75  MTGHHVTRRFGWDCHGLPVENEIDRKLN-IKRRDEVIKMGIDKYNEECRSIVTRYVAEWE 134

[Close]
BLAST of Carg20106 vs. TAIR10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-06
Identity = 28/85 (32.94%), Postives = 43/85 (50.59%), Query Frame = 0 

Query: 130 EPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQ 189
           E +I K W+    F    ++  G  F++   PP   G LHMGHA+   L+DI+ RY  + 
Sbjct: 78  EEKIYKWWESQGYFKPNFDQG-GSPFVIPMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMN 137

Query: 190 NYKVRFVPGWDCHGLPIELKGKKDL 215
                ++PG D  G+  +L  +K L
Sbjct: 138 GRPTLWLPGTDHAGIATQLVVEKML 161

[Close]
BLAST of Carg20106 vs. TAIR10
Match: AT1G09620.1 (ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases)

HSP 1 Score: 51.2 bits (121), Expect = 1.9e-06
Identity = 29/86 (33.72%), Postives = 39/86 (45.35%), Query Frame = 0 

Query: 130 EPEIQKIWDDHQVFNRVVERN---TGENFILHDGPPYANGDLHMGHALNKILKDIINRYK 189
           E  ++K W+D  VF      N    GE F      PY NG LH+GHA +    D  + Y 
Sbjct: 18  EATVRKWWEDEDVFRAESCENLPKPGEKFFSTFPFPYMNGYLHIGHAFSLSKVDFASAYH 77

Query: 190 LLQNYKVRFVPGWDCHGLPIELKGKK 213
            L+   V    G+ C G+PI+    K
Sbjct: 78  RLRGANVLLPFGFHCTGMPIKASADK 103

[Close]
BLAST of Carg20106 vs. Swiss-Prot
Match: sp|Q8RXK8|SYIM_ARATH (Isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA2 PE=2 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.8e-102
Identity = 222/447 (49.66%), Postives = 273/447 (61.07%), Query Frame = 0 

Query: 22  KGATKSLIQTSSYSVLSQRISAPLGRSY-IPSFRFRGSSSAKVFMFLSVTRYSTYLNDEF 81
           + A  +++Q+SSY VLS +  + L R+  + SF  +G SS K F FL V+R+ST  N+EF
Sbjct: 14  EAAAMAMVQSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSVKAFSFLYVSRFSTEPNNEF 73

Query: 82  LPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRANSTVREPEIQKIWDDH 141
             SSKRR+RGPVMAAKKAS+   + DGKYK TVDLPKT FGMRANS  REPE+QK+W+++
Sbjct: 74  GHSSKRRSRGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFGMRANSLTREPELQKLWEEN 133

Query: 142 QVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVRFVPGWD 201
           QVF RV + N G +FILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKV++VPGWD
Sbjct: 134 QVFKRVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWD 193

Query: 202 CHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT---------RYGVWADWNNPYL 261
           CHGLPIELK         +K+LTP+KLR KAAKFAKAT         R+GVWADWNNPYL
Sbjct: 194 CHGLPIELKVLQSLDQEVRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNNPYL 253

Query: 262 TLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFYVRLRCLVKWPFKDIFL 321
           TL PEYEAAQ+ +       F   + K ++               R R  V W       
Sbjct: 254 TLDPEYEAAQIEV-------FGQMALKGYI--------------YRGRKPVHW------- 313

Query: 322 EGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLF-LDWSRKVHLQAACYKNFF----QT 381
              S  T +   EL  P  +      I +  Y +F L    K  L      N +     T
Sbjct: 314 -SPSSRTALAEAELEYPEGH------ISKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTT 373

Query: 382 CAWPYGLQLLGLFQPMLVSSFLCYPVAVNAKLHYAIVELLAPSEDVSFLSGNKKKRPGNI 441
             W                      VAVNAKL Y++VE+ + SED S ++ NKKK PG +
Sbjct: 374 TPWTMPANA---------------AVAVNAKLQYSVVEVQSFSEDESTVTSNKKKIPGKV 410

Query: 442 LNEENRPFLVVASDLVPTLEAKWGVKL 446
           L  + + F++VA+DLVP LEAKWGVKL
Sbjct: 434 LKNQQKLFVIVATDLVPALEAKWGVKL 410

[Close]
BLAST of Carg20106 vs. Swiss-Prot
Match: sp|B0JSP4|SYI_MICAN (Isoleucine--tRNA ligase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=ileS PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.5e-58
Identity = 106/165 (64.24%), Postives = 122/165 (73.94%), Query Frame = 0 

Query: 109 YKHTVDLPKTTFGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDL 168
           YK TV+LP+T F MRAN++ REPEIQK W D Q++ ++ + N  E FILHDGPPYANG L
Sbjct: 7   YKDTVNLPQTDFDMRANASKREPEIQKFWQDEQIYEKLAQNNPKELFILHDGPPYANGSL 66

Query: 169 HMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLR 228
           H+GHALNKILKDIIN+YKLLQ YKVR+VPGWDCHGLPIELK         K+ LTP+KLR
Sbjct: 67  HIGHALNKILKDIINKYKLLQGYKVRYVPGWDCHGLPIELKVLQSLKSSEKEGLTPVKLR 126

Query: 229 DKAAKFAKAT---------RYGVWADWNNPYLTLSPEYEAAQVTI 257
            KA  FA  T         RYGVW DW NPYLTL PEYEAAQ+ +
Sbjct: 127 QKAHDFALQTQKEQCEGFKRYGVWGDWENPYLTLQPEYEAAQIAV 171

[Close]
BLAST of Carg20106 vs. Swiss-Prot
Match: sp|Q8YXY3|SYI_NOSS1 (Isoleucine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=ileS PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.5e-58
Identity = 106/167 (63.47%), Postives = 127/167 (76.05%), Query Frame = 0 

Query: 107 GKYKHTVDLPKTTFGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANG 166
           G YK TV+LPKT F MRAN+  REPEIQK W+++++F  + + N GE FILHDGPPYANG
Sbjct: 5   GSYKDTVNLPKTNFDMRANAIKREPEIQKFWEENKIFESLSQNNPGELFILHDGPPYANG 64

Query: 167 DLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIK 226
            LH+GHALNKILKDIINRY+LLQ  KVR+VPGWDCHGLPIELK         +++LTP++
Sbjct: 65  SLHIGHALNKILKDIINRYQLLQGRKVRYVPGWDCHGLPIELKVLQNLKSAERQNLTPLQ 124

Query: 227 LRDKAAKFAKAT---------RYGVWADWNNPYLTLSPEYEAAQVTI 257
           LR KA +FA AT         RYGVW DW+NPYLTL PEYEAAQ+ +
Sbjct: 125 LRQKAKEFALATVDNQRQNFKRYGVWGDWDNPYLTLKPEYEAAQIGV 171

[Close]
BLAST of Carg20106 vs. Swiss-Prot
Match: sp|B2IZP5|SYI_NOSP7 (Isoleucine--tRNA ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=ileS PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.9e-58
Identity = 103/167 (61.68%), Postives = 129/167 (77.25%), Query Frame = 0 

Query: 107 GKYKHTVDLPKTTFGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANG 166
           G YK TV+LPKT+F MRAN+  REPEIQK W+++++++R+ E N GE FILHDGPPYANG
Sbjct: 5   GSYKDTVNLPKTSFDMRANAIKREPEIQKFWEENKIYDRLFENNPGELFILHDGPPYANG 64

Query: 167 DLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIK 226
            LH+GHALNKILKDIINRY++L+  KVR+VPGWDCHGLPIELK         +++LTP++
Sbjct: 65  SLHIGHALNKILKDIINRYQMLRGRKVRYVPGWDCHGLPIELKVLQNMKSAERQNLTPLQ 124

Query: 227 LRDKAAKFAKAT---------RYGVWADWNNPYLTLSPEYEAAQVTI 257
           LR KA +F  AT         RYG+W DW+NPYLTL PEYEAAQ+ +
Sbjct: 125 LRQKAKEFGLATVDNQRQNFKRYGIWGDWDNPYLTLKPEYEAAQIGV 171

[Close]
BLAST of Carg20106 vs. Swiss-Prot
Match: sp|B1WVA3|SYI_CYAA5 (Isoleucine--tRNA ligase OS=Cyanothece sp. (strain ATCC 51142) OX=43989 GN=ileS PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.9e-56
Identity = 102/165 (61.82%), Postives = 122/165 (73.94%), Query Frame = 0 

Query: 109 YKHTVDLPKTTFGMRANSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDL 168
           YK TV+LPKT F MRAN+  REPE+QK W D+Q++ ++ + N  E F+LHDGPPYANG L
Sbjct: 7   YKDTVNLPKTNFSMRANAVKREPELQKFWADNQIYEKLSQENPEEVFVLHDGPPYANGSL 66

Query: 169 HMGHALNKILKDIINRYKLLQNYKVRFVPGWDCHGLPIEL--------KGKKDLTPIKLR 228
           HMGHALNK LKDIIN+YKLL+ YKVR+VPGWDCHGLPIEL        K ++ LTP+KLR
Sbjct: 67  HMGHALNKTLKDIINKYKLLRGYKVRYVPGWDCHGLPIELKVLQSMKSKEREGLTPLKLR 126

Query: 229 DKAAKFAKAT---------RYGVWADWNNPYLTLSPEYEAAQVTI 257
            KA  FA  T         R+GVW DW NPYLTL+PEYEAAQ+ +
Sbjct: 127 HKARDFALKTQEEQAKGFKRFGVWGDWENPYLTLTPEYEAAQIGV 171

[Close]
BLAST of Carg20106 vs. TrEMBL
Match: tr|A0A1S3AY52|A0A1S3AY52_CUCME (isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103483844 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 5.4e-149
Identity = 301/467 (64.45%), Postives = 324/467 (69.38%), Query Frame = 0 

Query: 5   NMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSSAKVF 64
           NMPLYASFAAGSSA KCK ATK  IQT SY+VLSQRIS+ L RSYI SFRFRGSSSAKVF
Sbjct: 4   NMPLYASFAAGSSAFKCKEATKPFIQTPSYTVLSQRISSTLRRSYISSFRFRGSSSAKVF 63

Query: 65  MFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRA 124
            FL+VTRYSTYLNDEF PSSKRR+RGP+MAAKKAS+ETNEGDGKYKHTVDLPKTTFGMRA
Sbjct: 64  TFLTVTRYSTYLNDEFPPSSKRRSRGPIMAAKKASQETNEGDGKYKHTVDLPKTTFGMRA 123

Query: 125 NSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 184
           NS VREPEIQKIWDDHQVFNR VERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR
Sbjct: 124 NSLVREPEIQKIWDDHQVFNRAVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 183

Query: 185 YKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT------ 244
           YKLLQNYKVRFVPGWDCHGLPIELK         +KDLTPIKLRDKAAKFAKAT      
Sbjct: 184 YKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMA 243

Query: 245 ---RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFY 304
              RYGVWADWNNPYLTLSPEYEAAQ+ +                      + +  Y F 
Sbjct: 244 SFKRYGVWADWNNPYLTLSPEYEAAQIEV-------------------FGQMAIQGYIF- 303

Query: 305 VRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSRKVHL 364
            R R  V W          S  T +   EL  P                       + H 
Sbjct: 304 -RGRKPVHW--------SPSSRTALAEAELEYP-----------------------EGHT 363

Query: 365 QAACYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYAIVELL 424
             + Y  F      P    +L  F P + ++ +   P        VAVNAKLHYAI+ELL
Sbjct: 364 SRSIYAIFRLVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAIIELL 418

Query: 425 APSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           APSED S L+GNKKK+PGNIL E  RPFLVVASDLVPTLEAKWGVK+
Sbjct: 424 APSEDASVLTGNKKKKPGNILKEGKRPFLVVASDLVPTLEAKWGVKI 418

[Close]
BLAST of Carg20106 vs. TrEMBL
Match: tr|A0A0A0L5T5|A0A0A0L5T5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G154310 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.1e-114
Identity = 214/250 (85.60%), Postives = 220/250 (88.00%), Query Frame = 0 

Query: 5   NMPLYASFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPLGRSYIPSFRFRGSSSAKVF 64
           NMPLYASFAAGSSA KCK ATK  IQT SY+VLSQRIS+ L RSYI SFRFRGSSSAKVF
Sbjct: 4   NMPLYASFAAGSSAFKCKEATKPFIQTPSYTVLSQRISSTLRRSYISSFRFRGSSSAKVF 63

Query: 65  MFLSVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRA 124
            FL+VTRYSTYLNDEF PSSKRR+RGP+MAAKKASKETNEGDGKYKHTVDLPKT FGMRA
Sbjct: 64  TFLTVTRYSTYLNDEFPPSSKRRSRGPIMAAKKASKETNEGDGKYKHTVDLPKTAFGMRA 123

Query: 125 NSTVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 184
           NS VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR
Sbjct: 124 NSLVREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINR 183

Query: 185 YKLLQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT------ 238
           YKLLQNYKVRFVPGWDCHGLPIELK         +KDLTPIKLRDKAAKFAKAT      
Sbjct: 184 YKLLQNYKVRFVPGWDCHGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMA 243

[Close]
BLAST of Carg20106 vs. TrEMBL
Match: tr|M5XXX4|M5XXX4_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa000564mg PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.3e-114
Identity = 248/467 (53.10%), Postives = 286/467 (61.24%), Query Frame = 0 

Query: 9   YASFAAGSSALKCKGATKSLIQTSSYSVLSQR-ISAPLGRSYIPSFRFRGSSSAKVFMFL 68
           + SFA  S + K   AT ++IQTS Y VLSQR  S+    + +  F FR  SS KVF   
Sbjct: 3   FKSFATHSFSFKPSEATMAVIQTSPYRVLSQRTCSSFRSTASVGLFYFRDRSSVKVFSLF 62

Query: 69  SVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRANST 128
            +  +S+Y NDEF  SSKRR+RGPVMAAKKA++   + DGKYKHTVDLPKT+FGMRANS 
Sbjct: 63  HMAHHSSYSNDEFASSSKRRSRGPVMAAKKAAEGAKQEDGKYKHTVDLPKTSFGMRANSL 122

Query: 129 VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKL 188
           +REPEIQKIWDD QVF RVV +NTGENFILHDGPPYANGDLH+GHALNKILKD INRYKL
Sbjct: 123 IREPEIQKIWDDSQVFKRVVGKNTGENFILHDGPPYANGDLHIGHALNKILKDFINRYKL 182

Query: 189 LQNYKVRFVPGWDCHGLPIELKGK-----------KDLTPIKLRDKAAKFAKAT------ 248
           LQNYKV +VPGWDCHGLPIELKGK           +DLTPIKLR KAAKFAK T      
Sbjct: 183 LQNYKVHYVPGWDCHGLPIELKGKYFLQSLDQAARRDLTPIKLRQKAAKFAKQTVKTQME 242

Query: 249 ---RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFY 308
              RYGVWADWNNPYLTL PEYEAAQ+ +            F   V         +  F 
Sbjct: 243 SFKRYGVWADWNNPYLTLDPEYEAAQIEV------------FGQMV---------IQGFI 302

Query: 309 VRLRCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSRKVHL 368
            R R  V W          S  T +   EL  P                       + H+
Sbjct: 303 YRGRKPVHW--------SPSSRTALAEAELEYP-----------------------EGHV 362

Query: 369 QAACYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYAIVELL 428
             + Y  F    A P    LL  + P + ++ +   P        VAVNAKL YAIVE+ 
Sbjct: 363 SRSIYAIFKLVSASPTSGGLLNEYFPNVCLAIWTTTPWTIPANAAVAVNAKLIYAIVEVQ 417

Query: 429 APSEDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           +  EDVS   GNKK+RPGN+L EEN+PFL+VASDLVP LEAKWGVKL
Sbjct: 423 SDPEDVSLSDGNKKRRPGNVLKEENKPFLIVASDLVPALEAKWGVKL 417

[Close]
BLAST of Carg20106 vs. TrEMBL
Match: tr|A0A251QX18|A0A251QX18_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G136500 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.1e-113
Identity = 246/464 (53.02%), Postives = 285/464 (61.42%), Query Frame = 0 

Query: 9   YASFAAGSSALKCKGATKSLIQTSSYSVLSQR-ISAPLGRSYIPSFRFRGSSSAKVFMFL 68
           + SFA  S + K   AT ++IQTS Y VLSQR  S+    + +  F FR  SS KVF   
Sbjct: 3   FKSFATHSFSFKPSEATMAVIQTSPYRVLSQRTCSSFRSTASVGLFYFRDRSSVKVFSLF 62

Query: 69  SVTRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRANST 128
            +  +S+Y NDEF  SSKRR+RGPVMAAKKA++   + DGKYKHTVDLPKT+FGMRANS 
Sbjct: 63  HMAHHSSYSNDEFASSSKRRSRGPVMAAKKAAEGAKQEDGKYKHTVDLPKTSFGMRANSL 122

Query: 129 VREPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKL 188
           +REPEIQKIWDD QVF RVV +NTGENFILHDGPPYANGDLH+GHALNKILKD INRYKL
Sbjct: 123 IREPEIQKIWDDSQVFKRVVGKNTGENFILHDGPPYANGDLHIGHALNKILKDFINRYKL 182

Query: 189 LQNYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT--------- 248
           LQNYKV +VPGWDCHGLPIELK         ++DLTPIKLR KAAKFAK T         
Sbjct: 183 LQNYKVHYVPGWDCHGLPIELKVLQSLDQAARRDLTPIKLRQKAAKFAKQTVKTQMESFK 242

Query: 249 RYGVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFYVRL 308
           RYGVWADWNNPYLTL PEYEAAQ+ +            F   V         +  F  R 
Sbjct: 243 RYGVWADWNNPYLTLDPEYEAAQIEV------------FGQMV---------IQGFIYRG 302

Query: 309 RCLVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSRKVHLQAA 368
           R  V W          S  T +   EL  P                       + H+  +
Sbjct: 303 RKPVHW--------SPSSRTALAEAELEYP-----------------------EGHVSRS 362

Query: 369 CYKNFFQTCAWPYGLQLLGLFQP-MLVSSFLCYP--------VAVNAKLHYAIVELLAPS 428
            Y  F    A P    LL  + P + ++ +   P        VAVNAKL YAIVE+ +  
Sbjct: 363 IYAIFKLVSASPTSGGLLNEYFPNVCLAIWTTTPWTIPANAAVAVNAKLIYAIVEVQSDP 414

Query: 429 EDVSFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           EDVS   GNKK+RPGN+L EEN+PFL+VASDLVP LEAKWGVKL
Sbjct: 423 EDVSLSDGNKKRRPGNVLKEENKPFLIVASDLVPALEAKWGVKL 414

[Close]
BLAST of Carg20106 vs. TrEMBL
Match: tr|A0A2P6QYB2|A0A2P6QYB2_ROSCH (Putative isoleucine--tRNA ligase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0422271 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.0e-112
Identity = 239/461 (51.84%), Postives = 287/461 (62.26%), Query Frame = 0 

Query: 11  SFAAGSSALKCKGATKSLIQTSSYSVLSQRISAPL-GRSYIPSFRFRGSSSAKVFMFLSV 70
           +F + +S+   + AT ++IQT+ Y VLSQR  +   G + +  F FRG SSAK F   ++
Sbjct: 2   NFKSLASSSSREAATMAMIQTTPYKVLSQRTRSTFRGTASVGLFYFRGRSSAKEFSLFNI 61

Query: 71  TRYSTYLNDEFLPSSKRRARGPVMAAKKASKETNEGDGKYKHTVDLPKTTFGMRANSTVR 130
           T YST+ +DEF  SSKRR+RGPVMAAKKA++   + DGKYKHTVDLPKT+FGMRANS+ R
Sbjct: 62  THYSTHSSDEFASSSKRRSRGPVMAAKKAAEGGKQQDGKYKHTVDLPKTSFGMRANSSTR 121

Query: 131 EPEIQKIWDDHQVFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQ 190
           EPE+QKIW+D+QVF RVV +NTGENFILHDGPPYANGDLH+GHALNKILKD+INRYKLLQ
Sbjct: 122 EPELQKIWEDNQVFKRVVSKNTGENFILHDGPPYANGDLHIGHALNKILKDMINRYKLLQ 181

Query: 191 NYKVRFVPGWDCHGLPIELK--------GKKDLTPIKLRDKAAKFAKAT---------RY 250
           NYKV +VPGWDCHGLPIELK         +KDLTPIKLR KAAKFAK T         RY
Sbjct: 182 NYKVHYVPGWDCHGLPIELKVLQSLDQAARKDLTPIKLRAKAAKFAKQTVKNQMESFKRY 241

Query: 251 GVWADWNNPYLTLSPEYEAAQVTIACILMFYFLCASFKHFVAELNSIVLCLYCFYVRLRC 310
           G+WADWNNPYLTL PEYEAAQ+ +            F   V         L  F  R R 
Sbjct: 242 GIWADWNNPYLTLDPEYEAAQIEV------------FGQMV---------LQGFIYRGRK 301

Query: 311 LVKWPFKDIFLEGESQFTGVPHHELLLPRLNWSTLRGILQEAYMLFLDWSRKVHLQAACY 370
            V W          S  T +   EL  P                       + H+  + Y
Sbjct: 302 PVHW--------SPSSRTALAEAELEYP-----------------------ESHVSRSIY 361

Query: 371 KNFFQTCAWPYGLQLLGLFQPMLVSSFLCYP--------VAVNAKLHYAIVELLAPSEDV 430
             F    A P    LL  +  + ++ +   P        VAVNAKL YAIVE+ + SEDV
Sbjct: 362 TAFKLVSAPPTSGGLLNEYPDVCLAIWTTTPWTIPANAAVAVNAKLIYAIVEVQSVSEDV 410

Query: 431 SFLSGNKKKRPGNILNEENRPFLVVASDLVPTLEAKWGVKL 446
           S  +GN K+ P N L EE +PFL+VASDLVPTLEAKWG+KL
Sbjct: 422 SSSAGNSKQTPANFLKEEKKPFLIVASDLVPTLEAKWGLKL 410

The following BLAST results are available for this feature:
BLAST of Carg20106 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022956209.11.1e-16470.06isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata][more]
XP_022979679.12.6e-16369.21isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima][more]
XP_023527875.12.6e-16369.92isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo... [more]
XP_008438885.18.2e-14964.45PREDICTED: isoleucine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo][more]
XP_011651056.18.2e-14965.10PREDICTED: LOW QUALITY PROTEIN: probable isoleucine--tRNA ligase, mitochondrial ... [more]
BLAST of Carg20106 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G49030.19.7e-10449.66tRNA synthetase class I (I, L, M and V) family protein[more]
AT4G10320.16.5e-1531.65tRNA synthetase class I (I, L, M and V) family protein[more]
AT5G16715.11.1e-0632.94ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... [more]
AT1G09620.11.9e-0633.72ATP binding;leucine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP b... [more]
BLAST of Carg20106 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8RXK8|SYIM_ARATH1.8e-10249.66Isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
sp|B0JSP4|SYI_MICAN3.5e-5864.24Isoleucine--tRNA ligase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN... [more]
sp|Q8YXY3|SYI_NOSS13.5e-5863.47Isoleucine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
sp|B2IZP5|SYI_NOSP75.9e-5861.68Isoleucine--tRNA ligase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX... [more]
sp|B1WVA3|SYI_CYAA51.9e-5661.82Isoleucine--tRNA ligase OS=Cyanothece sp. (strain ATCC 51142) OX=43989 GN=ileS P... [more]
BLAST of Carg20106 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3AY52|A0A1S3AY52_CUCME5.4e-14964.45isoleucine--tRNA ligase, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=... [more]
tr|A0A0A0L5T5|A0A0A0L5T5_CUCSA1.1e-11485.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G154310 PE=4 SV=1[more]
tr|M5XXX4|M5XXX4_PRUPE4.3e-11453.10Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa000564mg PE=3 SV=1[more]
tr|A0A251QX18|A0A251QX18_PRUPE2.1e-11353.02Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G136500 PE=3 SV=1[more]
tr|A0A2P6QYB2|A0A2P6QYB2_ROSCH4.0e-11251.84Putative isoleucine--tRNA ligase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g042... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0000166nucleotide binding
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
Vocabulary: INTERPRO
TermDefinition
IPR001412aa-tRNA-synth_I_CS
IPR002300aa-tRNA-synth_Ia
IPR014729Rossmann-like_a/b/a_fold
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005575 cellular_component
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20106-RACarg20106-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 130..256
e-value: 2.2E-45
score: 157.0
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 133..258
e-value: 3.6E-30
score: 104.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..112
NoneNo IPR availablePANTHERPTHR42765FAMILY NOT NAMEDcoord: 99..256
NoneNo IPR availablePANTHERPTHR42765:SF1ISOLEUCINE--TRNA LIGASE, MITOCHONDRIALcoord: 99..256
NoneNo IPR availableSUPERFAMILYSSF52374Nucleotidylyl transferasecoord: 108..260
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 162..173